CN107296917B

CN107296917B - Traditional Chinese medicine composition for preventing and treating red leg disease of wood frogs

Info

Publication number: CN107296917B
Application number: CN201710478596.XA
Authority: CN
Inventors: 王桂芹; 郭贵良; 郑伟; 陈玉珂; 付大平; 冯伟志; 陈秀梅; 牛小天; 孙丽; 于林海
Original assignee: Jilin Agricultural University
Current assignee: Qingdao Blue Valley Biotechnology Co ltd
Priority date: 2017-06-22
Filing date: 2017-06-22
Publication date: 2020-10-30
Anticipated expiration: 2037-06-22
Also published as: CN107296917A

Abstract

The invention discloses a traditional Chinese medicine composition for treating red leg diseases of wood frogs, which comprises the following components in parts by weight: 50-55 parts of Chinese gall, 40-50 parts of coptis chinensis, 40-50 parts of myrobalan, 30-40 parts of scutellaria baicalensis, 45-50 parts of dark plum, 40-50 parts of brandy root, 40-45 parts of dandelion, 30-35 parts of Chinese violet, 30-40 parts of common andrographis herb, 20-25 parts of houttuynia cordata, 30-35 parts of celandine, 50-55 parts of glabrous greenbrier rhizome, 20-25 parts of sargentgloryvine stem, 20-25 parts of paris polyphylla, 20-30 parts of spreading hedyotis herb, 20-25 parts of field thistle and 20-30 parts of liquorice; the traditional Chinese medicine composition has the effects of activating blood circulation to dissipate blood stasis, inducing diuresis to alleviate edema, clearing heat and removing toxicity, resisting bacteria and killing insects, has a strong killing effect on bacteria such as aeromonas hydrophila and the like which mainly cause the red leg disease of the wood frogs, and improves the immunity of the wood frogs, thereby achieving the purpose of curing the red leg disease of the wood frogs, and has the advantages of low cost, easy preparation, no toxic or side effect and obvious curative effect.

Description

Traditional Chinese medicine composition for preventing and treating red leg disease of wood frogs

Technical Field

The invention belongs to the field of economic animal breeding, and particularly relates to a traditional Chinese medicine composition for preventing and treating red leg diseases of wood frogs.

Background

The red leg disease of wood frog is also called septicemia, and is a common disease of tadpole, froglet and adult frog, and its pathogen is gram-negative bacteria of Aeromonas hydrophila and Acinetobacter calcoaceticus, etc., and said disease can be produced all the year round, but it can be produced in summer during raising period, and its infection is quick and death rate is high. The red leg disease of wood frog is a malignant infectious disease causing great loss in the whole artificial cultivation of wood frog. The disease is sudden and violent in incoming, can cause over 60 percent of death rate within 24-48h, completely dies in 5-7 days, and is one of the 'forest frog killer' diseases in the industry. The disease symptoms are lassitude of wood frogs, weakness of limbs, anorexia, redness of surfaces of lower jaw, abdomen and limbs, congestion, bleeding or ulceration of fingers and toes, and rapid death. The red leg disease is the most serious infectious disease which damages frogs, and the pathogeny is complex. The prevention and treatment of the disease have important significance for improving the yield and the quality of the wood frogs.

At present, the prevention and treatment of the red leg disease of the wood frogs are mainly carried out by water disinfection and feeding medicaments. Chinese patent CN97101685.2 "a technique for artificially breeding Chinese wood frogs", reports that the red legs of the wood frogs can be prevented and treated by sprinkling medicines such as 'Yukang' and 'Yuxian' in pool water; deng Yun Hua et al in one instance of diagnosis and treatment of red leg disease of wood frog mention that the wood frog red leg disease is taken orally and the medicine can be immediately stopped and dissolved and mixed with fly maggot for continuous feeding for 5-7 days, and the frog is gradually opened to eat; 1000 times of toxin-killing liquid made in English is sprayed for external use for 4 times a day, so that the death number of frogs is gradually reduced, and the disease condition is effectively controlled.

Disclosure of Invention

The invention aims to provide the traditional Chinese medicine composition for preventing and treating the red leg disease of the wood frog, which is easy to obtain materials, convenient to prepare, low in price and high in curative effect, and reduces medicine residues and toxic and side effects.

A traditional Chinese medicine composition for preventing and treating red leg diseases of wood frogs comprises the following components in parts by weight: 50-55 parts of Chinese gall, 40-50 parts of coptis chinensis, 40-50 parts of myrobalan, 30-40 parts of scutellaria baicalensis, 45-50 parts of dark plum, 40-50 parts of brandy root, 40-45 parts of dandelion, 30-35 parts of Chinese violet, 30-40 parts of common andrographis herb, 20-25 parts of houttuynia cordata, 30-35 parts of celandine, 50-55 parts of glabrous greenbrier rhizome, 20-25 parts of sargentgloryvine stem, 20-25 parts of paris polyphylla, 20-30 parts of spreading hedyotis herb, 20-25 parts of field thistle and 20-30 parts of liquorice;

the preferable weight components are as follows: 50 parts of gallnut, 40 parts of coptis chinensis, 40 parts of myrobalan, 30 parts of scutellaria baicalensis, 50 parts of dark plum fruit, 50 parts of brandy root, 40 parts of dandelion, 30 parts of Chinese violet, 30 parts of common andrographis herb, 20 parts of heartleaf houttuynia herb, 30 parts of celandine, 50 parts of glabrous greenbrier rhizome, 20 parts of sargentgloryvine stem, 20 parts of paris polyphylla, 20 parts of spreading hedyotis herb, 20 parts of common cephalanoplos herb and 20 parts of liquorice;

the preferable weight components are as follows: 50 parts of gallnut, 50 parts of coptis chinensis, 40 parts of myrobalan, 40 parts of scutellaria baicalensis, 50 parts of dark plum fruit, 40 parts of brandy root, 40 parts of dandelion, 35 parts of Chinese violet, 40 parts of common andrographis herb, 20 parts of houttuynia cordata, 35 parts of celandine, 55 parts of glabrous greenbrier rhizome, 25 parts of sargentgloryvine stem, 25 parts of paris polyphylla, 30 parts of spreading hedyotis herb, 25 parts of common cephalanoplos herb and 30 parts of liquorice;

the preferable weight components are as follows: 55 parts of gallnut, 50 parts of coptis chinensis, 50 parts of myrobalan, 40 parts of scutellaria baicalensis, 45 parts of dark plum fruit, 50 parts of brandy root, 40 parts of dandelion, 30 parts of Chinese violet, 40 parts of common andrographis herb, 25 parts of heartleaf houttuynia herb, 30 parts of celandine, 50 parts of glabrous greenbrier rhizome, 25 parts of sargentgloryvine stem, 25 parts of paris polyphylla, 30 parts of spreading hedyotis herb, 20 parts of common cephalanoplos herb and 20 parts of liquorice.

A preparation method of a traditional Chinese medicine composition for preventing and treating red leg diseases of wood frogs comprises the following steps:

weighing the components according to the raw material proportion of the traditional Chinese medicine composition for treating the red leg disease of the wood frogs, and crushing; soaking in 60-80% ethanol solution, concentrating the extractive solution, and recovering ethanol;

the concentration of the ethanol solution is 70%, the addition amount is preferably 3cm, and the soaking time is 5 days.

The invention provides a traditional Chinese medicine composition for treating red leg diseases of wood frogs, which comprises the following components in part by weight:

galla chinensis is also named as SANTANMEIYESHANGZI, Meretrix meretrix Linnaeus, BAICHONGCAO, MUFUZI, QIANGBEIZI, HONGYETAO, HANBEIZI, WUJIPAO, etc. Salty, sour, astringent and cold in nature. It enters lung, stomach and large intestine meridians. Has the effects of astringing lung and reducing fire, astringing intestine and checking diarrhea, arresting sweating, stopping bleeding, astringing dampness and healing sore, and the like. Modern medicine shows that gallnut has the function of stopping bleeding, and the decoction has obvious bacteriostatic or bactericidal effect on staphylococcus aureus, diplococcus pneumoniae, streptococcus, typhoid bacillus, dysentery bacillus, diphtheria bacillus, bacillus anthracis, pseudomonas aeruginosa and the like;

rhizoma Coptidis may be named as Yunlian, Yalian, Chuan Lian, Weilian, Jizhan, etc. Bitter in property and cold in nature. It enters heart, spleen, stomach, liver, gallbladder and large intestine meridians. Has effects in clearing away heat, eliminating dampness, purging pathogenic fire, and removing toxic materials. Modern pharmacological tests prove that the coptis chinensis has the effects of bacteriostasis, antivirus, resisting protozoan, reducing blood pressure, expanding coronary artery and the like;

terminalia chebula is named Terminalia chebula Leillei, Terminalia chebula Li and Ledebouriella root. Bitter, sour, astringent and neutral in nature. It enters lung and large intestine meridians. Has the effects of astringing intestines to check diarrhea, astringing lung to relieve cough, reducing pathogenic fire and relieving sore throat.

Radix Scutellariae is named as radix Camelliae Japonicae, radix Scutellariae tea, and radix Tujin tea. Bitter in property and cold in nature. It enters lung, gallbladder, spleen, large intestine and small intestine meridians. Has the effects of clearing heat, eliminating dampness, purging fire, removing toxic substances, stopping bleeding and the like.

The dark plum is named as dark plum, yellow boy, Hehan plum and dry branch plum. Sour and astringent taste, mild in nature. It enters liver, spleen, lung and large intestine meridians. Has the functions of astringing lung, astringing intestine, promoting fluid production, etc.

The radix Isatidis is radix Isatidis, and is also named as Isatis tinctoria, radix Isatidis, and radix Isatidis. Bitter in property and cold in nature. It enters liver and stomach meridians. Has the effects of clearing away heat and toxic materials, and cooling blood.

Herba Taraxaci, herba Urticae Cannabinae, herba Taraxaci, and herba Veronicae Didyma. Bitter and sweet in nature and cold in nature. It enters liver and stomach meridians. Has the effects of clearing away heat and toxic materials, promoting urination, and resolving hard mass. Now, the dandelion has the functions of antibiosis, lactation promotion, tumor resistance and cholagogue.

Herba Violae, arrowhead, herba Violae, herba Hyperici Japonici, herba Violae, cornu Cervi, herba Typhonii gigantei, herba Swertiae Bimaculatae, herba Violae, herba Cynomorii, and flos Calophylli. Bitter, pungent and cold in nature. It enters heart and liver meridians. Has the functions of clearing away heat and toxic material; cooling blood and relieving swelling.

The common andrographis herb is named as Chunlian autumn willow, the Chinese character of common andrographis herb, eleutherococcus, scabrous elephantfoot herb, serpentium, conus paniculata herb, Japanese climbing groundsel herb, tetragonia tetrandra, lysimachia christinae hance, sciadopitis, Chunlian summer willow, India grass and bittersweet herb. Has the functions of clearing away heat and toxic material, cooling blood and eliminating swelling.

Herba Houttuyniae is also called as herba Houttuyniae, radix Pleuroti Citrionae, herba Ainsliaeae Rubrinervis, herba Clerodendranthi Spicati, and herba Sapindi Japonicae. Pungent in nature and slightly cold in flavor. It enters lung meridian. Has effects in clearing away heat and toxic materials, eliminating carbuncle, expelling pus, promoting urination, and treating stranguria.

Herba Chelidonii, herba Dihuang, flos Daturae Metelis, and herba Clinopodii. Warm in nature, pungent and bitter in flavor. It enters lung and stomach meridians. Has effects in removing blood stasis, stopping bleeding, relieving spasm and pain, and relieving cough and asthma.

Smilax glabra is called as COO RICE ROLL, HARD RICE HEAD, HONGTULING, etc. Sweet, bland and mild in nature and taste. It enters liver and stomach meridians. Has effects in removing toxic materials, eliminating dampness, and smoothing joint movement.

Caulis Sargentodoxae is also called caulis Sargentodoxae. Bitter in property and mild in taste. Enter liver and large intestine meridians. Has the effects of removing toxin, eliminating carbuncle, promoting blood circulation, dredging collaterals, dispelling pathogenic wind and killing parasites.

Rhizoma paridis, radix Stephaniae Tetrandrae, rhizoma paridis, and fructus litchi. Bitter in nature and cool in taste. It enters heart, liver, lung, stomach and large intestine meridians. Has effects in removing toxic materials, resisting cancer, relieving swelling and pain, clearing away heat, arresting convulsion, relieving cough, and relieving asthma.

Herba Hedyotidis Diffusae is called herba Hedyotidis Diffusae, herba Eupatorii chinensis, and herba Hedyotidis Diffusae. Has the effects of clearing away heat and toxic materials, promoting blood circulation, and promoting urination. Modern pharmacological studies prove that the ophioglossum haplocalyx can enhance the immunity of organisms and inhibit the growth of tumor cells, has an inhibiting effect on pathogenic bacteria such as pseudomonas aeruginosa, staphylococcus aureus, pneumococcus and dysentery bacillus, and is a good medicine for clearing heat and removing toxicity.

Herba Cephalanoploris is also called "spiny" herb. Sweet in nature and taste and cool. Enter heart, small intestine and bladder meridians. Has effects of cooling blood, stopping bleeding, promoting blood circulation, removing toxic substance, inducing diuresis, and treating stranguria.

The liquorice is also named as Mesona chinensis Benth, sweet, mealy, petasites japonicus Benth, Lingtong, meadow rue herb, sweet grass, radix glycyrrhizae, stringy stonecrop herb, stringy herb and the like, has moderate nature and sweet taste, and enters spleen, stomach and lung channels. Has effects in clearing away heat and toxic materials, moistening lung, relieving cough, and regulating functions of the above medicines.

The preparation method is characterized in that: the recipe has the combined effects of promoting blood circulation to disperse blood clots, inducing diuresis to alleviate edema, clearing away heat and toxic material, resisting bacteria and killing pests.

The invention provides a traditional Chinese medicine composition for treating red leg diseases of wood frogs, which comprises the following components in parts by weight: 50-55 parts of Chinese gall, 40-50 parts of coptis chinensis, 40-50 parts of myrobalan, 30-40 parts of scutellaria baicalensis, 45-50 parts of dark plum, 40-50 parts of brandy root, 40-45 parts of dandelion, 30-35 parts of Chinese violet, 30-40 parts of common andrographis herb, 20-25 parts of houttuynia cordata, 30-35 parts of celandine, 50-55 parts of glabrous greenbrier rhizome, 20-25 parts of sargentgloryvine stem, 20-25 parts of paris polyphylla, 20-30 parts of spreading hedyotis herb, 20-25 parts of field thistle and 20-30 parts of liquorice; the components are organically combined, dialectically matched and mutually assisted, so that the effects of promoting blood circulation to remove blood stasis, inducing diuresis to alleviate edema, clearing heat and removing toxicity, resisting bacteria and killing insects and the like of the formula are enhanced, the gallnut in the formula is highly sensitive to aeromonas hydrophila and the like, and has a strong killing effect on bacteria which mainly cause the red leg disease of the wood frogs, such as the aeromonas hydrophila and the like, and the formula can effectively improve the immunity of the wood frogs, so that the aim of curing the red leg disease of the wood frogs is fulfilled. The traditional Chinese medicine composition for preventing and treating the red leg disease of the wood frogs has the advantages of low cost, easiness in preparation, no toxic or side effect and remarkable curative effect. Clinical practice proves that the medicine has the cure rate of 93.7 percent and the effective rate of 100 percent.

Detailed Description

Example 1

A traditional Chinese medicine composition for treating red leg disease of wood frogs comprises the following components by weight: 50 parts of gallnut, 40 parts of coptis chinensis, 40 parts of myrobalan, 30 parts of scutellaria baicalensis, 50 parts of dark plum fruit, 50 parts of brandy root, 40 parts of dandelion, 30 parts of Chinese violet, 30 parts of common andrographis herb, 20 parts of houttuynia cordata, 30 parts of celandine, 50 parts of glabrous greenbrier rhizome, 20 parts of sargentgloryvine stem, 20 parts of paris polyphylla, 20 parts of spreading hedyotis herb, 20 parts of common cephalanoplos herb and 20 parts of liquorice.

Example 2

A traditional Chinese medicine composition for treating red leg disease of wood frogs comprises the following components by weight: 50 parts of gallnut, 50 parts of coptis chinensis, 40 parts of myrobalan, 40 parts of scutellaria baicalensis, 50 parts of dark plum fruit, 40 parts of brandy root, 40 parts of dandelion, 35 parts of Chinese violet, 40 parts of common andrographis herb, 20 parts of houttuynia cordata, 35 parts of celandine, 55 parts of glabrous greenbrier rhizome, 25 parts of sargentgloryvine stem, 25 parts of paris polyphylla, 30 parts of spreading hedyotis herb, 25 parts of common cephalanoplos herb and 30 parts of liquorice.

Example 3

A traditional Chinese medicine composition for treating red leg disease of wood frogs comprises the following components by weight: 55 parts of gallnut, 50 parts of coptis chinensis, 50 parts of myrobalan, 40 parts of scutellaria baicalensis, 45 parts of dark plum fruit, 50 parts of brandy root, 40 parts of dandelion, 30 parts of Chinese violet, 40 parts of common andrographis herb, 25 parts of heartleaf houttuynia herb, 30 parts of celandine, 50 parts of glabrous greenbrier rhizome, 25 parts of sargentgloryvine stem, 25 parts of paris polyphylla, 30 parts of spreading hedyotis herb, 20 parts of common cephalanoplos herb and 20 parts of liquorice.

Example 4

Weighing the components according to the proportion of example 1, 2 or 3, the traditional Chinese medicine composition for treating the red leg disease of the wood frogs is prepared by the following method:

weighing the components according to the raw material proportion of the traditional Chinese medicine composition for preventing and treating the red leg disease of the wood frogs, removing impurities, quickly washing with cold boiled water, and draining; then sun drying the above obtained raw materials, and pulverizing into fine powder. The cold soaking method comprises placing pulverized medicinal materials into a soaking pot, adding 70% ethanol with the addition amount of 3cm below the medicinal powder, covering, soaking for 5 days, and shaking or stirring frequently during soaking to accelerate leaching. Then taking out the maceration extract, filtering, squeezing the residue, and mixing the obtained squeezed solution with the leachate. Placing the residue in an impregnator, adding 70% ethanol, soaking for 5 days, squeezing the residue, and mixing the obtained squeezed solution with the leachate. Mixing the two soaking solutions, filtering again, concentrating and removing ethanol to obtain the traditional Chinese medicine composition for preventing and treating the red leg disease of the wood frogs.

Experimental example 5

The using method comprises the following steps: every 100kg of wood frog feed (yellow mealworm, fly maggot, earthworm, etc.) is mixed with 1kg of a Chinese medicinal composition for preventing and treating red leg disease of wood frog. The prepared traditional Chinese medicine composition is added with a proper amount of alpha-starch and water as an adhesive and stirred uniformly, so that the medicine can be well adhered to the feed and is easy to be absorbed by wood frogs. The mixed feed is fed to wood frog with initial disease symptoms twice a day for 7 days.

Clinical curative effect experiments are carried out. Selecting 5 units such as a sand source wood frog cultivation farmer professional cooperative, a carbon stone city land forest frog cultivation farm, a carbon stone city wood river wood frog cultivation farm and the like from a Temple reservoir in Yongji county, and selecting 20 cases with wood frog red leg disease from 2005 + 2016 for testing. 300 early-stage diseased wood frogs are selected in each test and are evenly bred in 3 breeding ponds with the same conditions, namely a treatment pond, a norfloxacin treatment pond and a control pond.

The invention is characterized in that the medicine for treating the wood frog red leg disease prepared according to the embodiments 3 and 4 is stirred into the feed for feeding, 1 time respectively in the morning and afternoon every day, and the use time is 7 days continuously.

Use of norfloxacin treatment pool: norfloxacin fine powder and alpha-starch are uniformly mixed to the extent that the norfloxacin fine powder can be effectively adhered to feed. 1g of norfloxacin was added to 1kg of feed. The composition is administered 1 time each day in the morning and afternoon, and 7 days each time.

The control group was fed with normal feed and no other treatment was performed.

TABLE 1 grouping of diseased wood frogs and main treatment method

Group of	Affected wood frog quantity (only)	Method of treatment
			Group of the invention	100	The medicines are stirred into the feed for feeding, 1 time each in the morning and afternoon every day, and the medicine is continuously used for 7 days.
Norfloxacin treatment group	100	Stirring norfloxacin into the feed, and feeding the norfloxacin into the feed 1 time each day in the morning and afternoon, wherein the norfloxacin is continuously used for 7 days.
			Control group	100	And feeding the normal feed without other treatment.

The curative effect standard is as follows: mainly depends on whether the symptoms of the red leg disease of the wood frogs disappear. The medicine is taken for 7 days as a course of treatment, and the specific curative effect is divided into three levels:

and (3) curing: the red leg disease symptoms of the wood frogs disappear completely, and the activities and the appetite recover to be normal;

improvement: the red leg disease symptoms of the wood frogs mostly disappear, and the activity and appetite of the wood frogs are greatly improved;

and (4) invalidation: the red leg disease symptoms of the wood frogs do not disappear, or a small part of the red leg disease symptoms disappear, and the activities and the appetite are not obviously improved.

The specific therapeutic effects of the test groups are shown in Table 2. As can be seen from Table 2, the two groups of medicinal baits have different treatment effects on the wood frogs, wherein the treatment effect of the group of the invention is the best, the cure rate reaches 95.00 percent, the total effective rate is 100 percent, the total effective rate is remarkably higher than that of the commonly used norfloxacin treatment group (P is less than 0.01), and the control group has no treatment effect.

TABLE 2 therapeutic Effect of the various treatment regimens

Group of	Number of treatments (only)	Cure of disease	Improvement of life	Invalidation	Cure rate (%)	Total effective rate (%)
							Group of the invention	100	95	5	0	95.00	100.00
Norfloxacin treatment group	100	70	15	15	70.00	85.00
							Control group	100	-	-	50	0	0

Practice proves that the medicine provided by the invention organically combines Chinese herbal medicines, dialectically combines the medicines and assists the Chinese herbal medicines with each other, enhances the effects of the formula on activating blood circulation, removing blood stasis, inducing diuresis, relieving swelling, clearing heat, removing toxicity, resisting bacteria and killing insects, and has a good curative effect on the red leg disease of the wood frogs, thereby achieving the purpose of healing.

The externally applied medicine for treating the wood frog red leg disease, which is prepared by the preparation method of the Chinese medicinal composition for preventing and treating the wood frog red leg disease, has the advantages of low cost, easy preparation, no toxic or side effect and remarkable curative effect. Provides a feasible new medicine for clinically treating the disease. Clinical practice proves that the cure rate of the medicine is 95.00 percent, and the effective rate is 100 percent.

The detailed description of the medicine for treating red leg disease of wood frog and the preparation method thereof with reference to the embodiments are illustrative and not restrictive, and several embodiments can be enumerated according to the limited scope, so that changes and modifications without departing from the general concept of the present invention shall fall within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine composition for preventing and treating red leg diseases of wood frogs comprises the following components in parts by weight: 50-55 parts of Chinese gall, 40-50 parts of coptis chinensis, 40-50 parts of myrobalan, 30-40 parts of scutellaria baicalensis, 45-50 parts of dark plum, 40-50 parts of brandy root, 40-45 parts of dandelion, 30-35 parts of Chinese violet, 30-40 parts of common andrographis herb, 20-25 parts of houttuynia cordata, 30-35 parts of celandine, 50-55 parts of glabrous greenbrier rhizome, 20-25 parts of sargentgloryvine stem, 20-25 parts of paris polyphylla, 20-30 parts of spreading hedyotis herb, 20-25 parts of field thistle and 20-30 parts of liquorice.

2. The traditional Chinese medicine composition for preventing and treating the red leg disease of the wood frogs as claimed in claim 1, which comprises the following components in parts by weight: 50 parts of gallnut, 40 parts of coptis chinensis, 40 parts of myrobalan, 30 parts of scutellaria baicalensis, 50 parts of dark plum fruit, 50 parts of brandy root, 40 parts of dandelion, 30 parts of Chinese violet, 30 parts of common andrographis herb, 20 parts of houttuynia cordata, 30 parts of celandine, 50 parts of glabrous greenbrier rhizome, 20 parts of sargentgloryvine stem, 20 parts of paris polyphylla, 20 parts of spreading hedyotis herb, 20 parts of common cephalanoplos herb and 20 parts of liquorice.

3. The traditional Chinese medicine composition for preventing and treating the red leg disease of the wood frogs as claimed in claim 1, which comprises the following components in parts by weight: 50 parts of gallnut, 50 parts of coptis chinensis, 40 parts of myrobalan, 40 parts of scutellaria baicalensis, 50 parts of dark plum fruit, 40 parts of brandy root, 40 parts of dandelion, 35 parts of Chinese violet, 40 parts of common andrographis herb, 20 parts of houttuynia cordata, 35 parts of celandine, 55 parts of glabrous greenbrier rhizome, 25 parts of sargentgloryvine stem, 25 parts of paris polyphylla, 30 parts of spreading hedyotis herb, 25 parts of common cephalanoplos herb and 30 parts of liquorice.

4. The traditional Chinese medicine composition for preventing and treating the red leg disease of the wood frogs as claimed in claim 1, which comprises the following components in parts by weight: 55 parts of gallnut, 50 parts of coptis chinensis, 50 parts of myrobalan, 40 parts of scutellaria baicalensis, 45 parts of dark plum fruit, 50 parts of brandy root, 40 parts of dandelion, 30 parts of Chinese violet, 40 parts of common andrographis herb, 25 parts of heartleaf houttuynia herb, 30 parts of celandine, 50 parts of glabrous greenbrier rhizome, 25 parts of sargentgloryvine stem, 25 parts of paris polyphylla, 30 parts of spreading hedyotis herb, 20 parts of common cephalanoplos herb and 20 parts of liquorice.

5. A preparation method of a traditional Chinese medicine composition for preventing and treating red leg diseases of wood frogs comprises the following steps: the traditional Chinese medicine composition for preventing and treating the red leg disease of the wood frogs as claimed in claim 1 is prepared by weighing and crushing the components; soaking in 60-80% ethanol solution, concentrating the extractive solution, and recovering ethanol.

6. The preparation method of the traditional Chinese medicine composition for preventing and treating the red leg disease of the wood frogs as claimed in claim 5, which is characterized in that: the concentration of the ethanol solution is 70 percent, and the dipping time is 5 days.