CN107281417A - Treat the Chinese medicine composition of the protrasion of the lumbar intervertebral disci and its preparation method of medicament - Google Patents

Treat the Chinese medicine composition of the protrasion of the lumbar intervertebral disci and its preparation method of medicament Download PDF

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Publication number
CN107281417A
CN107281417A CN201710682994.3A CN201710682994A CN107281417A CN 107281417 A CN107281417 A CN 107281417A CN 201710682994 A CN201710682994 A CN 201710682994A CN 107281417 A CN107281417 A CN 107281417A
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China
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chinese medicine
medicine composition
root
filtrate
chinese
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姜宏
刘锦涛
朱宇
俞鹏飞
李晓春
钱祥
钟鸣
俞振翰
刘冠虹
马智佳
戴锋
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Suzhou Hospital of Traditional Chinese Medicine
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Suzhou Hospital of Traditional Chinese Medicine
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481—Astragalus (milkvetch)
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/62—Leeches; Worms, e.g. cestodes, tapeworms, nematodes, roundworms, earth worms, ascarids, filarias, hookworms, trichinella or taenia
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    • A61K36/06—Fungi, e.g. yeasts
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    • A61K36/06—Fungi, e.g. yeasts
    • A61K36/07—Basidiomycota, e.g. Cryptococcus
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    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/232—Angelica
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    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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    • A61K2236/50—Methods involving additional extraction steps
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Abstract

Present invention is disclosed a kind of preparation method for the Chinese medicine composition and its medicament for treating the protrasion of the lumbar intervertebral disci, wherein, the Chinese medicine composition includes：The raw 60g of the Radix Astragali 30, the 20g of Poria cocos 10, the 20g of umbellate pore furgus 10, the 20g of the root of Chinese clematis 15, the 20g of pawpaw 12, the 12g of dried orange peel 6, the 20g of RADIX ANGELICAE SINEINSIS 15, the 30g of Ligusticum wallichii 20, the boiling hot 12g of leech 9, the 12g of earthworm 9, the 30g of Semen Coicis Semen Coicis 15, the 30g of the root of fangji 15, the 9g of semen brassicae 6, the 20g of rhizoma atractylodis macrocephalae 10, the 20g of radix cyathulae 10.The Chinese medicine composition can be made into Chinese herbs decoction or mixture.The present invention can promote prominent interverbebral disc reabsorption, so as to reach the purpose of the treatment protrasion of the lumbar intervertebral disci.

Description

Treat the Chinese medicine composition of the protrasion of the lumbar intervertebral disci and its preparation method of medicament
Technical field
The present invention relates to the field of Chinese medicines, more particularly, to a kind of Chinese medicine composition and its medicine for treating the protrasion of the lumbar intervertebral disci The preparation method of agent.
Background technology
The protrasion of the lumbar intervertebral disci is clinically common disease, frequently-occurring disease and the course of disease is longer.It wherein there are about 10%~20% trouble Person needs operative treatment, and in the U.S., statistics has 200,000 people's row lumbar intervertebral disc operations every year.Domestic someone is domestic every by statistical inference It there are about million intervertebral disc operations year.
Guinto in 1984 carries out follow-up to the expectant treatment result of patients with prolapse of lumbar intervertebral disc, is observed first with CT The reabsorption phenomenon of Lumbar intervertebral disc protrusion.In recent years, absorbed naturally without operative treatment after external relevant protrusion of lumber intervertebral disc Clinical report is increasing, and its result demonstrates the presence of Lumbar intervertebral disc protrusion absorbing phenomenon, is clinically expectant treatment interverbebral disc The validity and reasonability of herniation provide theoretical foundation.Numerous studies show, after protrusion of lumber intervertebral disc reabsorption with it is newborn Blood vessel, granulation tissue, macrophage, TNF, a variety of inflammatory cell hormones are both participated in intervertebral disc hernial tissue Phagocytosis digestion, that is, the process absorbed is a process that immune lysis is carried out to protrusion.Traditional Chinese medicine " is treated naturally as a kind of Method ", has few, the evident in efficacy advantage of cheap, toxic side effect in terms of the treatment protrasion of the lumbar intervertebral disci promotes its reabsorption.
It is more common in clinic with research on treating lumbar disc herniation by TCM at present, but there is not yet use Chinese medicine lumbar vertebrae Disc herniation is so as to promote the re-absorbed research of its protrusion.
The content of the invention
The protrasion of the lumbar intervertebral disci is treated it is an object of the invention to overcoming the defect of prior art there is provided one kind to promote The preparation method of the re-absorbed Chinese medicine composition of its protrusion and its medicament.
To achieve the above object, the present invention proposes following technical scheme：A kind of Chinese medicine group for treating the protrasion of the lumbar intervertebral disci Compound, the Chinese medicine composition includes：Raw Radix Astragali 30-60g, Poria cocos 10-20g, umbellate pore furgus 10-20g, root of Chinese clematis 15-20g, pawpaw 12-20g, dried orange peel 6-12g, RADIX ANGELICAE SINEINSIS 15-20g, Ligusticum wallichii 20-30g, boiling hot leech 9-12g, earthworm 9-12g, Semen Coicis Semen Coicis 15- 30g, root of fangji 15-30g, semen brassicae 6-9g, rhizoma atractylodis macrocephalae 10-20g, radix cyathulae 10-20g.
Preferably, the Chinese medicine composition includes：Raw Radix Astragali 40-50g, Poria cocos 15-20g, umbellate pore furgus 15-20g, the root of Chinese clematis 15-18g, pawpaw 15-20g, dried orange peel 10-12g, RADIX ANGELICAE SINEINSIS 15-20g, Ligusticum wallichii 20-30g, scald leech 10-12g, earthworm 10-12g, Semen Coicis Semen Coicis 20-30g, root of fangji 20-30g, semen brassicae 6-8g, rhizoma atractylodis macrocephalae 15-20g, radix cyathulae 15-20g.
Preferably, the Chinese medicine composition includes：Raw Radix Astragali 45g, Poria cocos 18g, umbellate pore furgus 16g, root of Chinese clematis 16g, pawpaw 18g, dried orange peel 11g, RADIX ANGELICAE SINEINSIS 17g, Ligusticum wallichii 25g, scald leech 11g, earthworm 10g, Semen Coicis Semen Coicis 26g, root of fangji 24g, semen brassicae 7g, Rhizoma atractylodis macrocephalae 16g, radix cyathulae 18g.
Preferably, the medicament that the Chinese medicine composition is made is Chinese herbs decoction.
Preferably, the medicament that the Chinese medicine composition is made is mixture.
Present invention also offers another technical scheme：A kind of system that Chinese drugs agentia is made of above-mentioned Chinese medicine composition Preparation Method, including：
S1, a course for the treatment of amount is weighed by the part by weight of each raw material of the Chinese medicine composition, by one course for the treatment of amount Chinese medicine composition is equally divided into two parts, and portion adds water 10~20 times of amounts, another 5~9 times of amount that add water；
S2, add water two parts of Chinese medicine compositions are decocted at least one times respectively, filtrate is filtrated to get after decoction, by two parts of filters Liquid merges, and the filtrate after merging is concentrated, and is concentrated into concentrate of the concentration range equivalent to 1~10g/ml crude drugs；
S3, the concentrate is added 2~6 times of amount alcohol contents be 60% ethanol, refrigeration stands 20~30h, filtration, to second Alcohol is without reclaiming filtrate after alcohol taste；
S4, boils the recovery filtrate, plus 2~6 grams of sodium benzoate, 0.2~0.4 gram of nipagin ethylester, filtration is let cool, plus Water is filling while hot to 400~600ml.
Preferably, the preparation method includes：
S1 ', a course for the treatment of amount is weighed by the part by weight of each raw material of the Chinese medicine composition, by one course for the treatment of amount Chinese medicine composition is equally divided into two parts, and portion adds water 10 times and measured, and another adds water 8 times and measured；
S2 ', add water two parts of Chinese medicine compositions are decocted twice respectively, filtrate is filtrated to get after decoction, by two parts of filtrates Merge, and the filtrate after merging is concentrated, be concentrated into concentrate of the concentration range equivalent to 5g/ml crude drugs；
S3 ', adds the ethanol that 3 times of amount alcohol contents are 60% by the concentrate, and refrigeration stands 24h, filtration, to ethanol without alcohol Filtrate is reclaimed after taste；
S4 ', boils the recovery filtrate, plus 3 grams of sodium benzoate, 0.25 gram of nipagin ethylester, filtration is let cool, and is added water to 500ml, it is filling while hot.
Preferably, in step S1, one course for the treatment of amount is 7 days.
Chinese medicine composition of the present invention is with QI invigorating Li Shui, by phlegm dredging collateral, and the medullization that disappears core is the rules for the treatment of, by the raw Radix Astragali, Poria cocos, pig Siberian cocklebur, the root of Chinese clematis, pawpaw, dried orange peel, RADIX ANGELICAE SINEINSIS, Ligusticum wallichii, boiling hot leech, earthworm, Semen Coicis Semen Coicis, the root of fangji, semen brassicae, rhizoma atractylodis macrocephalae, river ox Knee constitutes for medicine.In this group of medicine：
Raw Radix Astragali tonifying middle-Jiao and Qi, makes the prosperous then blood of gas, and dispersing blood stasis is monarch drug in a prescription, works as reuse without hindering just；
Poria cocos, umbellate pore furgus clearing damp and promoting diuresis power are strong, and Poria cocos can also invigorating the spleen calming heart, root of fangji dispelling wind and eliminating dampness, inducing diuresis for removing edema, Radix Angelicae Sinensis promoting blood circulation Disperse blood stasis and dredge collateral, without blood trouble, semen brassicae is longer than warming cold phlegm, profit gas dissipating bind, is apt to drive the outer phlegm of flesh side film, is altogether ministerial drug；
And Ligusticum wallichii is the gas medicine in blood, Radix Angelicae Sinensis promoting blood circulation is helped, and have an effect of promoting qi circulation and relieving pain, it is dried orange peel, Semen Coicis Semen Coicis, white Art, pawpaw help root of fangji invigorating the spleen dehumidifying, inducing diuresis for removing edema, and root of Chinese clematis wind-damp dispelling, softening and resolving hard mass disappear bone sticking, eliminate the nucleus pulposus of protrusion, water Leech, earthworm help semen brassicae reducing phlegm and resolving masses dredging collateral, the strong waist and knee of the root of bidentate achyranthes, strengthen the bone, and are adjutant.
All medicines share, makes exopathogen to remove, and water is wet must be capable, and phlegm-blood stasis must disappear, and QI-blood circulation is unobstructed, no pain by smooth breath, and all diseases can be cured.
Modern pharmacological research, the monarch drug in a prescription Radix Astragali and its compound can strengthen prominent nucleus pulposus and attract the T of activity, bone-marrow-derived lymphocyte Effect, by improving autoimmunity effect, and then promote the reabsorption after protrusion of lumber intervertebral disc；The active ingredient root of fangji in the root of fangji Alkali has the effect of anti-inflammatory diuresis consumer edema, can mitigate nerve root oedema, eliminates because being dashed forward caused by nucleus pulposus peripheral edema in iconography Go out thing increase；Radix Angelicae Sinensis polysaccharide promotes RBC acceptor garland rate, stimulates new vessels to grow into；Ligusticum wallichii active ingredient ligustrazine, expansible blood Pipe, scavenging activated oxygen；Bighead atractylodes rhizome invigorating the spleen to dry, it may have immunological regulation and inducing diuresis to remove edema effect；Earthworm, leech have promotion blood small Plate aggregation, anti-freezing, the sanguimotor effect of improvement.The root of Chinese clematis has an analgesic activity, and is pushed away according to its dissipating bind fish-bone fishbone effect that disappears Survey it also has certain dissolution to prominent nucleus pulposus；And the chymopapain extracted in pawpaw, it is used as already Nucleus pulposus lytic agent is used for the minimally-invasive treatment of the protrasion of the lumbar intervertebral disci；Semen brassicae is irritant, can stimulate quickening local blood circulation, Improve blood supply, promote new vessels generation.
In addition, Chinese medicine composition of the present invention all proves that it can promote through experiment in vivo, in vitro test and clinical observation Prominent interverbebral disc reabsorption, so as to reach the purpose of the treatment protrasion of the lumbar intervertebral disci.
Brief description of the drawings
Fig. 1 is the schematic flow sheet of Chinese medicine preparation preparation method of the present invention；
Magnetic resonance imaging schematic diagram when Fig. 2 is one first visit of case of the present invention；
Fig. 3 is magnetic resonance imaging schematic diagram after case of the present invention one follow-up April；
Magnetic resonance imaging schematic diagram when Fig. 4 is two first visit of case of the present invention；
Fig. 5 is magnetic resonance imaging schematic diagram after the follow-up August of case one of the present invention；
Fig. 6 is the reabsorption ratio schematic diagram of 95 patients of follow-up of the present invention；
Fig. 7 is the reabsorption ratio schematic diagram of 78 patients of follow-up of the present invention.
Embodiment
Below in conjunction with the accompanying drawing of the present invention, the technical scheme to the embodiment of the present invention carries out clear, complete description.
Embodiment 1
A kind of Chinese medicine composition for treating the protrasion of the lumbar intervertebral disci disclosed in the embodiment of the present invention 1, its raw material components And respective weight is：
Raw Radix Astragali 30g, Poria cocos 10g, umbellate pore furgus 10g, root of Chinese clematis 15g, pawpaw 12g, dried orange peel 6g, RADIX ANGELICAE SINEINSIS 15g, Ligusticum wallichii 20g, Scald leech 9g, earthworm 9g, Semen Coicis Semen Coicis 15g, root of fangji 15g, semen brassicae 6g, rhizoma atractylodis macrocephalae 10g, radix cyathulae 10g.
Above-mentioned Chinese medicine composition can be made into Chinese herbs decoction, may be made as mixture.
As shown in figure 1, the Chinese medicine composition of above-described embodiment 1 is made to the preparation method of Chinese drugs agentia, including：
Step S1, a course for the treatment of amount is weighed by the raw material in above-described embodiment 1, by the Chinese medicine composition of a course for the treatment of amount Two parts are equally divided into, portion adds water 10 times and measured, another adds water 5 times and measured；
Step S2, add water two parts of Chinese medicine compositions are decocted once respectively, filtrate is filtrated to get after decoction, by two parts of filters Liquid merges, and the filtrate after merging is concentrated, and is concentrated into concentrate of the concentration range equivalent to 1g/ml crude drugs；
Step S3, the ethanol for being 60% by concentrate plus 2 times of amount alcohol contents, refrigeration stands 20h, filtration, to ethanol without alcohol Filtrate is reclaimed after taste；
Step S4, boils recovery filtrate, plus 2 grams of sodium benzoate, 0.2 gram of nipagin ethylester, filtration is let cool, and is added water to 400ml, it is filling while hot.
Embodiment 2
A kind of Chinese medicine composition for treating the protrasion of the lumbar intervertebral disci disclosed in the embodiment of the present invention 2, its raw material components And respective weight is：
Raw Radix Astragali 60g, Poria cocos 20g, umbellate pore furgus 20g, root of Chinese clematis 20g, pawpaw 20g, dried orange peel 12g, RADIX ANGELICAE SINEINSIS 20g, Ligusticum wallichii 30g, boiling hot leech 12g, earthworm 12g, Semen Coicis Semen Coicis 30g, root of fangji 30g, semen brassicae 9g, rhizoma atractylodis macrocephalae 20g, radix cyathulae 20g.
Above-mentioned Chinese medicine composition can be made into Chinese herbs decoction, may be made as mixture.
As shown in figure 1, the Chinese medicine composition of above-described embodiment 2 is made to the preparation method of mixture, including：
Step S1, a course for the treatment of amount is weighed by the raw material in above-described embodiment 2, by the Chinese medicine composition of a course for the treatment of amount Two parts are equally divided into, portion adds water 20 times and measured, another adds water 9 times and measured；
Step S2, add water two parts of Chinese medicine compositions are decocted twice respectively, filtrate is filtrated to get after decoction, by two parts of filters Liquid merges, and the filtrate after merging is concentrated, and is concentrated into concentrate of the concentration range equivalent to 10g/ml crude drugs；
Step S3, the ethanol for being 60% by concentrate plus 6 times of amount alcohol contents, refrigeration stands 30h, filtration, to ethanol without alcohol Filtrate is reclaimed after taste；
Step S4, boils recovery filtrate, plus 6 grams of sodium benzoate, 0.4 gram of nipagin ethylester, filtration is let cool, and is added water to 600ml, it is filling while hot.
Embodiment 3
A kind of Chinese medicine composition for treating the protrasion of the lumbar intervertebral disci disclosed in the embodiment of the present invention 3, its raw material components And respective weight is：
Raw Radix Astragali 40g, Poria cocos 15g, umbellate pore furgus 15g, root of Chinese clematis 15g, pawpaw 15g, dried orange peel 10g, RADIX ANGELICAE SINEINSIS 15g, Ligusticum wallichii 20g, boiling hot leech 10g, earthworm 10g, Semen Coicis Semen Coicis 20g, root of fangji 20g, semen brassicae 6g, rhizoma atractylodis macrocephalae 15g, radix cyathulae 15g.
Above-mentioned Chinese medicine composition can be made into Chinese herbs decoction, may be made as mixture.
As shown in figure 1, the Chinese medicine composition of above-described embodiment 3 is made to the preparation method of mixture, including：
Step S1, a course for the treatment of amount is weighed by the raw material in above-described embodiment 3, by the Chinese medicine composition of a course for the treatment of amount Two parts are equally divided into, portion adds water 10 times and measured, another adds water 5 times and measured；
Step S2, add water two parts of Chinese medicine compositions are decocted once respectively, filtrate is filtrated to get after decoction, by two parts of filters Liquid merges, and the filtrate after merging is concentrated, and is concentrated into concentrate of the concentration range equivalent to 1g/ml crude drugs；
Step S3, the ethanol for being 60% by concentrate plus 2 times of amount alcohol contents, refrigeration stands 20h, filtration, to ethanol without alcohol Filtrate is reclaimed after taste；
Step S4, boils recovery filtrate, plus 2 grams of sodium benzoate, 0.2 gram of nipagin ethylester, filtration is let cool, and is added water to 400ml, it is filling while hot.
Embodiment 4
A kind of Chinese medicine composition for treating the protrasion of the lumbar intervertebral disci disclosed in the embodiment of the present invention 4, its raw material components And respective weight is：
Raw Radix Astragali 50g, Poria cocos 20g, umbellate pore furgus 20g, root of Chinese clematis 18g, pawpaw 20g, dried orange peel 12g, RADIX ANGELICAE SINEINSIS 20g, Ligusticum wallichii 30g, boiling hot leech 12g, earthworm 12g, Semen Coicis Semen Coicis 30g, root of fangji 30g, semen brassicae 8g, rhizoma atractylodis macrocephalae 20g, radix cyathulae 20g.
Above-mentioned Chinese medicine composition can be made into Chinese herbs decoction, may be made as mixture.
As shown in figure 1, the Chinese medicine composition of above-described embodiment 4 is made to the preparation method of mixture, including：
Step S1, a course for the treatment of amount is weighed by the raw material in above-described embodiment 4, by the Chinese medicine composition of a course for the treatment of amount Two parts are equally divided into, portion adds water 20 times and measured, another adds water 9 times and measured；
Step S2, add water two parts of Chinese medicine compositions are decocted twice respectively, filtrate is filtrated to get after decoction, by two parts of filters Liquid merges, and the filtrate after merging is concentrated, and is concentrated into concentrate of the concentration range equivalent to 10g/ml crude drugs；
Step S3, the ethanol for being 60% by concentrate plus 6 times of amount alcohol contents, refrigeration stands 30h, filtration, to ethanol without alcohol Filtrate is reclaimed after taste；
Step S4, boils recovery filtrate, plus 6 grams of sodium benzoate, 0.4 gram of nipagin ethylester, filtration is let cool, and is added water to 600ml, it is filling while hot.
Embodiment 5
A kind of Chinese medicine composition for treating the protrasion of the lumbar intervertebral disci disclosed in the embodiment of the present invention 5, its raw material components And respective weight is：
Raw Radix Astragali 45g, Poria cocos 18g, umbellate pore furgus 16g, root of Chinese clematis 16g, pawpaw 18g, dried orange peel 11g, RADIX ANGELICAE SINEINSIS 17g, Ligusticum wallichii 25g, boiling hot leech 11g, earthworm 10g, Semen Coicis Semen Coicis 26g, root of fangji 24g, semen brassicae 7g, rhizoma atractylodis macrocephalae 16g, radix cyathulae 18g.
Above-mentioned Chinese medicine composition can be made into Chinese herbs decoction, may be made as mixture.
As shown in figure 1, the Chinese medicine composition of above-described embodiment 5 is made to the preparation method of mixture, including：
Step S1, a course for the treatment of amount is weighed by the raw material in above-described embodiment 5, by the Chinese medicine composition of a course for the treatment of amount Two parts are equally divided into, portion adds water 10 times and measured, another adds water 8 times and measured；
Step S2, add water two parts of Chinese medicine compositions are decocted twice respectively, filtrate is filtrated to get after decoction, by two parts of filters Liquid merges, and the filtrate after merging is concentrated, and is concentrated into concentrate of the concentration range equivalent to 5g/ml crude drugs；
Step S3, adds the ethanol that 3 times of amount alcohol contents are 60% by above-mentioned concentrate, and refrigeration stands 24h, filtration, to ethanol Without filtrate is reclaimed after alcohol taste；
Step S4, boils above-mentioned recovery filtrate, plus 3 grams of sodium benzoate, 0.25 gram of nipagin ethylester, filtration is let cool, and is added water It is filling while hot to 500ml.
Embodiment 6
A kind of Chinese medicine composition for treating the protrasion of the lumbar intervertebral disci disclosed in the embodiment of the present invention 5, its raw material components And respective weight is：
Raw Radix Astragali 42g, Poria cocos 16g, umbellate pore furgus 18g, root of Chinese clematis 17g, pawpaw 16g, dried orange peel 11g, RADIX ANGELICAE SINEINSIS 16g, Ligusticum wallichii 26g, boiling hot leech 11g, earthworm 11g, Semen Coicis Semen Coicis 28g, root of fangji 26g, semen brassicae 7g, rhizoma atractylodis macrocephalae 18g, radix cyathulae 16g.
Above-mentioned Chinese medicine composition can be made into Chinese herbs decoction, may be made as mixture.
As shown in figure 1, the Chinese medicine composition of above-described embodiment 6 is made to the preparation method of mixture, including：
Step S1, a course for the treatment of amount is weighed by the raw material in above-described embodiment 6, by the Chinese medicine composition of a course for the treatment of amount Two parts are equally divided into, portion adds water 15 times and measured, another adds water 6 times and measured；
Step S2, add water two parts of Chinese medicine compositions are decocted twice respectively, filtrate is filtrated to get after decoction, by two parts of filters Liquid merges, and the filtrate after merging is concentrated, and is concentrated into concentrate of the concentration range equivalent to 8g/ml crude drugs；
Step S3, adds the ethanol that 4 times of amount alcohol contents are 60% by above-mentioned concentrate, and refrigeration stands 26h, filtration, to ethanol Without filtrate is reclaimed after alcohol taste；
Step S4, boils above-mentioned recovery filtrate, plus 4 grams of sodium benzoate, 0.3 gram of nipagin ethylester, filtration is let cool, and is added water to 450ml, it is filling while hot.
In the step S1 of above-described embodiment 1~6, a course for the treatment of amount is generally 7 days, certainly also can actual conditions determine one The specific cycle duration of the individual course for the treatment of.
In addition, used in the above embodiment of the present invention：
Raw Astragalus from different habitats is the Inner Mongol, is leguminous perennial herb astragalus mongolicus Astragalus Membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao root.
The root of fangji place of production is Zhejiang, is the perennial bejuco Fourstamen Stephania Root Stephania tetrandra of Menispermaceae S.Moore root.
The Radix Angelicae Sinensis place of production is Gansu, is umbelliferae angelica Angelica sinensis (Oliv.) Diels dry root.
The Ligusticum wallichii place of production is Sichuan, is Umbelliferae herbaceos perennial Ligusticum wallichii Ligusticum chuanxiong Hort. Rhizome.
The pawpaw place of production is Yunnan, is rose family machaka chaenomeles lagenaria Chaenomeles lagenaria (Loisel.) Koidz. with pawpaw (Quince short, bristly hair or beards) C.sinensis (Thouin) Koehne ripening fruits.
The earthworm place of production is Guangdong, is Annelida Ju Yin sections animal Pheretima aspergillum Pheretimaas pergilum (E Perrier), popular Pheretimatschiliensis P vulgarisChen, power simulation P guillelmi (Michaelsen) or comb the region between the heart and the diaphragm hair earthworm P pectiniferaMichaelsen hirudo leech.
The bighead atractylodes rhizome place of production is Zhejiang, is feverfew bighead atractylodes rhizome Atractylodes macrocephala Koidz. dry root Stem.
The root of Chinese clematis place of production is Jiangsu, is dicotyledon Ranunculaceae Ranunculaceae root ofs Chinese clematis Clematis ChinensisOsbeck, Clematis hexapetala (mountain knotweed) C.hexapetala Pall. drying root and rhizome.
The semen brassicae place of production is Anhui, is crucifer sinapsis alba Sinapis alba (L.) Boiss. or mustard B.juncea (L.) Czern.et Coss. mature seed.
The leech place of production is Jiangsu, is Hirudinidae animal leech Whitmania pigra Whitman, leech Hirudo Drying for nipponica Whitman or whitmania acranulata Whitman Whitmania acranulata Whitman is all.
The Poria cocos place of production is Dabie Mountain Jinzhai County, is the dry sclerotia for intending shelf fungus section fungi Poria cocos Wolfiporia cocos.
The umbellate pore furgus place of production is Gansu, is Aphyllophorales On Polyporaceae umbellate pore furgus Polyporus umbellatus (Pers.) Fries dry sclerotia.
The dried orange peel place of production be Hubei, be rutaceae orange Citrus reticulata Blanco and its variety into Ripe pericarp.
The Semen Coicis Semen Coicis place of production is Fujian, is the benevolence of benevolence grass Job's tears.
The radix cyathulae place of production is Sichuan, is amaranthaceous plant radix cyathulae Cyathula officinalis Kuan dry root.
The beneficial effect of Chinese medicine composition of the present invention is further illustrated below by test example, these experiments use this Mixture prepared by invention above-described embodiment carries out in vivo studies and in vitro test：
First, basic research
1st, laboratory apparatus：
The super digital display thermostat water bath of 501A types, BB5060 CO2Incubator, DDB full-automatic electronic peristaltic pumps, DHG- 907AH electric heating constant-temperature blowing drying boxes, DK-8D electric heating constant temperature tanks, FA2004N electronic analytical balances, FR-200A is automatically purple Outer visible analytical equipment, KA-1000 low speed desk centrifuges, KQ2200 ultrasonic cleaners, LD control group X-40 vertical electrics Pressure steam sterilization boiler, MK3 ELIASAs, MV--1 vertical slab electrophoresis grooves, PCR gene amplification instrument, SBE6300 electrophoresis apparatuses, The multi-function microplate readers of Synergy 2, TL-18M table-type high-speed refrigerated centrifuges, S-1 decolorization swinging tables, U410 ultra low temperature freezers, XSP-17C inverted biologic microscopes, adjustable pipette, ultra-pure water instrument, Tissue Culture Plate, ware, PCR gene amplification instrument is ultraviolet Spectrophotometer.
2nd, experiment reagent：
Total RNA extraction reagent box (DP419), chloroform, isopropanol, 75% ethanol pre-cooled ethanol, (high temperature goes out deionized water It is living), DEPC processing water, dNTP, random primer, M-MLV (200U/ μ L), RNAsin (40U/ μ L), Taq archaeal dna polymerases (5U/ μ L)；DNA marker(100bp)；Design of primers uses Primer premier3 software process qualities, and object of reference selects glyceraldehyde -3- Phosphate dehydrogenase (glyceraldehyde-3-phosphate dehydrogenase, GAPDH).Wherein,
IL-2：(amplification length 350bp) sense primer：5′-GCG CAC CCA CTT CAA GCC CT-3′；Draw in downstream Thing：5′-CCA CCA CAG TTG CTG GCT CA-3′.
TNF-α：(amplification length 363bp) sense primer：5′-TGA GCA CTG AAA GCA TGA TC-3′；Draw in downstream Thing：5′TTA TCT CTC AGC TCC ACG CC-3′.
VEGF：(amplification length 127bp) sense primer：5′-TGGACC CTG GCT TTA CTG CTG-3′；Draw in downstream Thing：5′-GCA ATA GCT GCG CTG GTAGA-3′.
Col2α1：(amplification length 112bp) sense primer：5′-TCC TAA GGG TGC CAA TGG TGA-3′；Downstream Primer：5′-AGG ACC AAC TTT GCC TTG AGA C-3′.
MMP3：(amplification length 369bp) sense primer：5′-CTC TTC CAT CCA ACA TGG ATA GCT G-3′； Anti-sense primer：5′-ACT CCA ACT GTG AAG ATC CGC TGA-3′.
MMP13：(amplification length 142bp) sense primer：5′-CCC TGG AGC CCT GAT GTT T-3′.
Beta-actin(GAPDH)：(amplification length 150bp) sense primer：5′-GGA GAT TAC TGC CCT GGC TCC TA-3′；Anti-sense primer：5′-GAC TCA TCG TAC TCC TGC TTG CTG-3′.
3rd, experiment material：
(1) experimental animal：
Three monthly age SPF grade SD male rats 126 are selected, body weight is in 300g or so, zoopery environment：Cleaning grade.
(2) experimental specimen：
Because protrasion of the lumbar intervertebral disci inpatient row routine operation treats the disc tissue extractd (including nucleus pulposus and fiber Ring) 30, wherein, rupture type is prominent 15, and (sample obtains and obtains patient and family members, Hospital Ethical Committee non-ruptured 15, type Agreement).
4th, experimental drug：
The Chinese medicine composition mixture that any one is prepared using above-described embodiment 1~6, it is preferred to use embodiment 5 is made The standby Chinese medicine composition mixture obtained.
Experimental animal is converted according to adult's dosage, and rat oral gavage is treated, concentration 1g/ml, 2.4ml/ times, daily It is administered once.After experimental animal modeling second day start administration, respectively at behind after administration the 10th day, 20 days, 30 days by rat Disc tissue detection that is dead and taking out heeling-in.
Take out and be dipped in after sample in the DMEM culture mediums containing volume fraction for 10% hyclone in art, insert ice chest fast Speed belt returns in laboratory, 3h and shreds carry out uterus tissue pieces, sets up reabsorption cell model after protrusion of lumber intervertebral disc.
SD rats 8, simple randomization is divided into two groups, every group 4, blank serum is prepared respectively and Chinese medicine composition mixture contains Medicine serum, by body surface area convert animal dose,equivalent, wiring solution-forming, administration group press the daily gavages of 2mL/kg, control group with The daily gavages of physiological saline 2mL/kg.The total arterial blood drawing of 1h collares of last gavage, extracts serum, 56 DEG C of water-bath inactivations, suction filtration It is degerming, packing, 20 DEG C save backup.
The rat disc of test example 1. protrude reabsorption during IL-2, TNF-α, VEGF, Col2 α l, MMP3, MMP13mRNA is expressed
Experiment packet and modeling
1. the acquisition of interverbebral disc：Rat shaves off back after the success of ketalar (0.10g/kg) intraperitoneal injection of anesthesia Chaeta.Rubber band ties every rat under afterbody, routine disinfection, aseptic condition and cuts the 3rd, 4 tail bone interverbebral discs two (comprising upper Lower cartilage endplate).Wherein model group I (interverbebral disc does not puncture group) interverbebral disc, which is not punctured, keeps its integrality, model group II (vertebras Disk punctures group), TNF-α inhibitor group, imuran group, Chinese medicine composition mixture interverbebral disc with 10mL syringe needles pierce Broken taken interverbebral disc center fibrous ring exposure nucleus pulposus；Claim its weight with electronic balance (a ten thousandth unit) and record, by intervertebral Disk is put into porose 200 μ L EP pipes.Empty EP pipes are implanted into muscle of back by sham-operation group.
The heeling-in of interverbebral disc：Iodophor routine disinfection back, posterior median line cuts skin, muscle, will be equipped with the EP of interverbebral disc Pipe；
Implantation muscle layer, subsequent layer-by-layer suture, wound is coated with erythromycin ophthalmic ointment.Rat is put into cage after modeling success Conventinal breeding.
2. the extracting of rat disc cells total serum IgE
Carried out using above-mentioned total RNA extraction reagent box (DP419)：
(1) the tail intervertebral disc of rat tissue being stored in liquid nitrogen is taken out, is shredded on ice；
(2) tissue sample shredded is moved into homogenate tube, 1ml lysates RZ is added immediately；
(3) 1min or so is quickly homogenized with homogenizer, homogenate is transferred in a new centrifuge tube without RNase, room Temperature stands 5 minutes；
(4) 200 μ L chloroforms are added, 15s is acutely vibrated, room temperature places 3min；
(5) 4 DEG C of 15000rpm centrifuge 10min；
(6) go supernatant 0.5ml to add 0.5ml isopropanols to mix, room temperature places 10min
(7) add in adsorption column CR3,4 DEG C of 15000rpm centrifuge 30s, discard the waste liquid in pipe；
(8) 500 μ L protein liquid removals RD, 4 DEG C of 15000rpm centrifugation 30s are added into adsorption column CR3, waste liquid is abandoned；
(9) 700 μ L rinsing liquid RW are added into adsorption column CR3,2min is stored at room temperature, 4 DEG C of 15000rpm centrifuge 30s, abandoned Waste liquid；
(10) 500 μ L rinsing liquids RW, 4 DEG C of 15000rpm centrifugation 30s are added into adsorption column CR3, waste liquid is abandoned；
(11) adsorption column is put into 2ml collecting pipes, 4 DEG C of 15000rpm centrifuge 2min, remove residual liquid；
(12) adsorption column is transferred in a new centrifuge tube, adds 25 μ LDEPC processing water dissolvings, be to extract out RNA, -70 DEG C of preservations.
3.RNA identification
Identified using above-mentioned UV detector：
This 5 μ L of sampling are added in quartz cuvette, are added distilled water 0.8ml, are fully mixed, compareed with distilled water.In purple 260,280nm wavelength even an OD value, record are read on outer spectrophotometer.Calculate：
It is 40 μ gRNA, so being calculated as follows when OD260 is 1：
Sample rna content (μ g/ μ L)=OD260 × 160 × 40/1000
As OD260/OP280 ＜ 1.8, the pollution for having albumen or phenol is pointed out, it is necessary to which RNA is further purified.
As OD260/OP280 ＞ 1.8, show that purity is qualified.
Reverse transcription RNA → cDNA
(1) test tube is marked, is sequentially added：
(2) 25 DEG C of 10min, 42 DEG C of 1h, 75 DEG C of 10min reverse transcriptions are that cDNA is transcribed into cDNA.
4. real-time fluorescence quantitative PCR reacts
Reaction system uses ToYoBo Realtime PCR Master Mix (SYBR Green) (QPK-201), total anti- It is 25 μ l to answer volume.
(1) real-time fluorescence quantitative PCR reaction system
Thermal circulation parameters：
The annealing temperature of VEGF, actin gene is 62 DEG C, and MMP13 is 64 DEG C, and Col10 α 1 are 56 DEG C, and remaining is 60 ℃.Sample is denatured 5 minutes through 95 DEG C, first carries out 3 circulations, then carries out 40 cyclic amplifications, glimmering in the detection of each circulation Luminous intensity.After all amplification cycles terminate, the melting curve for carrying out 80 circulations detects to determine the specificity of PCR primer.
Real-time fluorescence quantitative PCR interpretation of result：
The relative expression quantity of target gene can be using Δ Δ CT methods (difference for referring to CT values between different groups) analysis, with just Normal group is used as check sample.(the target gene CT- of observation sample is seen Δ Δ CT=observation sample Δ CT- check sample Δs CT= Examine sample internal reference CT)-(the target gene CT- check samples internal reference CT of check sample).The relative expression quantity of sample=2- Δ Δs CT。
5. experimental result：IL-2 in each group, TNF-α, VEGF, Col2 α 1, MMP3, MMP13mRNA relative expression quantity such as Under：
IL-2, TNF-α, VEGF, Col2 α 1, MMP3, MMP13mRNA relative expression quantity, such as following table one in 10 days each groups：
Table one
Note：One-way analysis of variance, Chinese medicine composition mixture group is compared P ＜ 0.05 with model I；Model group II and model I Group compares P ＜ 0.05.
IL-2, TNF-α, VEGF, Col2 α 1, MMP3, MMP13mRNA relative expression quantity, see the table below two in 20 days each groups：
Table two
Note：One-way analysis of variance, Chinese medicine composition mixture is compared P ＜ 0.05 with model I；Model group II and model I groups Compare P ＜ 0.05；Chinese medicine composition mixture is compared P ＜ 0.05 for general group with benefit match；Chinese medicine composition mixture and imuran group ratio Compared with P ＜ 0.05；Model group II is compared P ＜ 0.05 for general group with benefit match.
IL-2, TNF-α, VEGF, Col2 α 1, MMP3, MMP13mRNA relative expression quantity, see the table below three in 30 days each groups：
Table three
Note：One-way analysis of variance, Chinese medicine composition mixture is compared P ＜ 0.05 with model I；Model group II and model I groups Compare P ＜ 0.05；Chinese medicine composition mixture is compared P ＜ 0.05 for general group with benefit match；Chinese medicine composition mixture and imuran group ratio Compared with P ＜ 0.05；Model group II is compared P ＜ 0.05 for general group with benefit match；Model group II is compared P ＜ 0.05 with imuran group.
The study find that detecting IL-2, TNF-α, VEGF, Col2 α in each group nucleus pulposus of Each point in time 1st, MMP3, MMP13mRNA are expressed, and over time, expression quantity gradually increases.Wherein, Chinese medicine composition mixture group is to be measured Gene mRNA expression amount is most.Chinese medicine composition mixture can substantially increase testing gene mRNA expression, so as to promote to protrude intervertebral The reabsorption of disk.
The Chinese medicine composition mixture of test example 2. influences on co-cultured cell MMP-3, MMP-7, MMP-13
Experiment packet and modeling
1. original cuiture
It is in aseptic superclean bench, the tissue removed is complete with PBS liquid 3 blood stains into disc tissue block of flushing Portion is cleaned, and distinguishes fibrous ring and nucleus pulposus, tissue block is shredded to about 0.5mm × 0.5mm × 0.5mm, is placed in a diameter of 10cm's Tissue Culture Dish bottom, keeps fragment to be spaced about 1mm, adds the DMEM culture medium 1.5ml that volume fraction is 20%FBS, is placed in Volume fraction is that the DMEM trainings for adding that volume fraction is 20%FBS after 3h, 3h are cultivated in 5%,CO2 37 DEG C of cell culture incubators Based 1.5 ml is supported, avoids tissue block from floating as far as possible, adds 100u/mL containing penicillin, streptomysin 100mg/L.It is every that liquid is changed in first three time Change once within three days, change liquid once within every two days after cell is grown.
2. Secondary Culture
When primary cell about 90% is paved with bottom of bottle, nutrient solution is sucked, PBS liquid is gently rinsed 3 times, add 2ml or so 0.25% trypsase and 0.01%EDTA mixed liquors, jog culture dish make digestive juice cover all cell surfaces, 2~ 4min adds a little 10%FBS nutrient solutions and terminates digestion after seeing that kytoplasm retraction, cell rounding, most cells suspend under mirror； Capillary syring gently blows and beats ware parietal cell and is allowed to come off, and is transferred in 15cm centrifuge tube, is put into centrifuge, 1000r/min, from Heart 5min, removes supernatant；The DMEM culture mediums that volume fraction is 20%FBS are added, cell suspension is uniformly dripped respectively Enter on the slide in 3 six orifice plate Tissue Culture Dish, about 1ml cell suspension is filled on each slide, cell is gently placed in Continue by former condition of culture to cultivate in incubator, inverted microscope is placed in after 4 hours, and to observe visible most cells adherent, then Secondary addition culture medium about 1.5ml, changes liquid once in every two days.
3. human macrophage culture
The recovery of cell：
(1) carry out alcohol in superclean bench before testing and clean preparation, and water-bath pot temperature is adjusted to 37 DEG C.
(2) the target cell strain frozen is taken out with thickening gloves from liquid nitrogen, in the water-bath at a temperature of 37 DEG C constantly Shaking, is allowed to melt as early as possible.
(3) cryopreservation tube of thawing is used with rapid move to after 75% wipes of alcohol wash disinfection under superclean bench, aseptic condition Liquid-transfering gun draws cell suspension, moves into sterilized centrifuge tube, adds 5mL corresponding nutrient solution, gently blows even.
(4) cell suspension 1000rpm centrifuges 8min, abandoning supernatant.
(5) cell is diluted to 105cells/mL cell suspension with corresponding nutrient solution, is inoculated in Tissue Culture Flask.
(6) cell bottle is put in CO2In incubator, 5%CO2Under the conditions of 37 DEG C culture.The nutrient solution renewed for second day, so Cultivate in a conventional manner afterwards.
Passage culture：
10cm Tissue Culture Dish will be inoculated in after human macrophage strain recovery, it is 10%FBS's to add 8ml volume fractions 1640 culture mediums, are placed in volume fraction for 5%CO237 DEG C of cell culture incubators in cultivate.Macrophage is half attached cell, is changed 2/3 nutrient solution that gentle aspiration upper strata turns yellow during liquid, adds the fresh medium of equivalent.Treat cell quantity length to 10x106 When, capillary syring gently blows afloat ware parietal cell, is allowed to suspend, is transferred in 15cm centrifuge tube, is put into centrifuge, 600r/ Min, centrifuges 5min, removes supernatant；1640 culture mediums that volume fraction is 10%FBS are added, cell suspension is uniformly divided It Di Ru not add in appropriate culture medium, cell culture incubator and to continue to cultivate by former condition of culture in 3 10cm Tissue Culture Dish.
Cell freezes：
(1) cell is carried out Secondary Culture by 24-48h before cell cryopreservation, it is ensured that cell is in exponential phase of growth.
(2) frozen stock solution is prepared：RPMI1640: serum: the mixed liquor of DMSO=7: 2: 1 (volume ratio).
(3) capillary syring gently blows afloat ware parietal cell, is allowed to suspend, is transferred in 15cm centrifuge tube, is put into centrifuge, 600r/min, centrifuges 5min, removes supernatant.
(4) precipitated and mixed with the cells frozen storing liquid dissolving configured, be then transferred in cryopreservation tube.
(5) it is cell microbiological contamination during reduction cell cryopreservation, cryopreservation tube is sealed with sealed membrane, while being carried out with marking pen Mark, freezes.4 DEG C of storages 15min, -20 DEG C of storage 40min, after cell suspension freezes, -80 DEG C are deposited one day, are finally frozen In liquid nitrogen.
Experiment packet：
(1) blank control group：Independent intervertebral disc cells culture
(1) NS control groups：Independent intervertebral disc cells culture
(3) low dose group：Cell adds 5% Chinese medicine composition mixture Contained Serum
(4) middle dose group：Cell adds 5% Chinese medicine composition mixture Contained Serum
(5) high dose group：Cell adds 5% Chinese medicine composition mixture Contained Serum
(6) observing time is divided into 12h, 24h, tri- time points of 48h.
2. experimental result：
The MMP-3 protein expressions of each group intervertebral disc cells different time points
Group 12h 24h 48h
Blank control 0.488±0.091*△▲★□ 0.467±0.055*△▲★□ 0.575±0.043*△▲★□
NS is compareed 0.457 ± 0.025* △ ▲ ★ mouthfuls 0.602±0.046△▲★□ 0.713 ± 0.062 △ ▲ ★ mouthfuls
Low dose group 0.607 ± 0.041 △ ▲ ★ mouthfuls 1.275 ± 0.025 ▲ mouth 0.904±0.018△▲★□
Middle dose group 0.898±0.053△▲★□ 2.367±0.031 2.175±0.057▲□
High dose group 0.987 ± 0.060 △ ▲ ★ mouthfuls 3.612±0.046 3.723±0.052△★
Table four
Note：* P ＜ 0.05, VS 24h low dose groups；△ P ＜ 0.05, VS 24h middle dose groups；▲ P ＜ 0.05, VS 24h High dose group；★ P ＜ 0.05, VS 48h middle dose groups；P ＜ 0.05, VS 48h high dose groups.
The MMP-7 protein expressions of each group intervertebral disc cells different time points
Group 12h 24h 48h
Blank control 0.288±0.001*△▲★□ 0.267±0.081*△▲★□ 0.275±0.036*△▲★□
NS is compareed 0.287±0.048*△▲★□ 0.312 ± 0.006* △ ▲ ★ mouthfuls 0.303±0.009*△▲★□
Low dose group 0.527 ± 0.021* △ ▲ ★ mouthfuls 0.875 ± 0.014 ▲ mouth 0.904±0.018▲★□
Middle dose group 0.808 ± 0.083 ▲ ★ mouthfuls 1.367 ± 0.073 mouthful 1.715±0.071
High dose group 1.987±0.037 2.112±0.006 2.623±0.029△
Table five
Note：* P ＜ 0.05, VS 12h high dose groups；△ P ＜ 0.05, VS 24h middle dose groups；▲ P ＜ 0.05, VS 24h High dose group；★ P ＜ 0.05, VS 48h middle dose groups；P ＜ 0.05, VS 48h high dose groups.
The MMP-13 protein expressions of each group intervertebral disc cells different time points
Group 12h 24h 48h
Blank control 0.388±0.010*Δ 0.363±0.018*△ 0.357±0.006*Δ
NS is compareed 0.417±0.055*Δ 0.312±0.016*Δ 0.348±0.019*Δ
Low dose group 0.527±0.061*△ 0.675±0.074 0.704±0.064
Middle dose group 0.788±0.036 0.967±0.059 0.975±0.011
High dose group 0.987±0.080 1.612±0.056 1.823±0.024
Table six
Note：* P ＜ 0.05, VS 24h high dose groups；Δ P ＜ 0.05, VS 48h high dose groups.
Conclusion：The study find that macrophage can be promoted to act on interverbebral disc is thin for certain density Chinese medicine composition mixture Born of the same parents, make interverbebral disc stromatin enzyme family MMPs height expression, accelerate interverbebral disc substrate degradation, so as to promote reabsorption, further Effect of this side is demonstrated from molecular cytology angle, the party promotes re-absorbed mechanism to provide scientific basis for research.
2nd, model case is illustrated
Validity of this side in terms of patient symptom is improved is confirmed through a large amount of Clinical Follow-up observational studies, and is conducive to intervertebral After protruding reabsorption occurs for disk.
Case one：Patient male, first visit 48 years old occurs pain in the back under without obvious inducement and led and the day of right lower extremity more than 20, straight legs 30 ° (+) in experiment right side is raised, JOA scores 4 points, patient's refusal operation, therefore give Yiqi Huoxue Recipe traditional Chinese medicine therapy for treatment, follow-up 4 MRI contrasts find that protrusion disappears substantially before and after month, and patient clinical symptom complete incidence graph, and JOA scorings are increased to 29 points, follow-up So far conditions of patients is not recurred, and quality of life is significantly improved.As shown in Figure 2 a and 2 b, MRI (magnetic resonance imaging, English during first visit Literary full name Magnetic Resonance Imaging) the visible huge protrusion of interverbebral disc；As shown in Figure 3 a and Figure 3 b shows, follow-up April Afterwards, MRI shows that protrusion disappears substantially.
Case two：What so-and-so, female, 42 years old, individual shop-assistant, because the protrasion of the lumbar intervertebral disci moves in our hospital's orthopedics and traumatology, was admitted to hospital When JOA score 6 points, be admitted to hospital MRI as shown in figures 4 a and 4b, prominent rate 100% gives Yiqi Huoxue TCM prescription same during being in hospital Shi Peihe dexamethasone mannitol dehydration therapies, symptom is left hospital recuperation after taking a turn for the better, and symptom gradually takes a turn for the better disappearance, August after bed 8 weeks Checked afterwards to our hospital, JOA scores 28 points, it checks MRI as shown in figure 5 a and 5b, prominent rate is 10%, prominent before and after treatment Thing absorptivity 90%.Iconography surrounding time was at intervals of 8 months.As shown in figures 4 a and 4b, the visible interverbebral discs of MRI are huge during first visit It is big prominent with upper extension；As shown in figure 5 a and 5b, after August, MRI shows that protrusion is wholly absent.
3rd, case Follow-up results are reported
This team is carried out for the clinical efficacy of the Yiqi Huoxue TCM prescription therapeutic protrasion of the lumbar intervertebral disci for a long time A large amount of follow-up observations.
Find there are 21 (70%) contract after 30 protrusion of lumber intervertebral disc patients of the initial follow-up of this team, expectant treatment It is small, wherein 5 are obviously reduced.Subsequent 95 protrusion of lumber intervertebral disc patients of follow-up again, respectively with Chinese medicine Yiqi Huoxue Recipe And western medicine, follwing-up in average 6 months, Chinese medicine group JOA 8 improvement rates of scoring and MRI protrusion absorptivities are substantially better than Western medicine group. Wherein 10 occur in that reabsorption rate ＞ 50%.It is easier to occur reabsorption because rupture type is protruded, therefore for Study of Traditional Chinese Medicine Prescription promotes re-absorbed curative effect after rupture type protrusion, and 78 rupture type protrusion of lumber intervertebral disc patients of follow-up, passes through Chinese medicine group Side intervenes, and follow-up re-absorbed ratio occurs up to 81% after finding disc herniation.In addition for teenager's protrusion of lumber intervertebral disc Patient, Chinese prescription promotes also have unique advantage in terms of protrusion diminution for improving clinical symptoms, especially.
It can be seen that this Chinese prescription is improving protrusion of lumber intervertebral disc patient clinical symptom, is particularly promoting rupture type to protrude generation There is unique advantage and good efficacy in terms of reabsorption.
Wherein, in 95 protrusion of lumber intervertebral disc patients of follow-up, as shown in fig. 6, Yiqi Huoxue TCM prescription therapeutic occurs Reabsorption ratio is up to 29%.78 rupture type protrusion of lumber intervertebral disc patients of follow-up, as shown in fig. 7, Yiqi Huoxue TCM prescription is controlled Reabsorption ratio occurs for interverbebral disc up to 81% after treatment.
The technology contents and technical characteristic of the present invention have revealed that as above, but those skilled in the art still may base Make a variety of replacements and modification without departing substantially from spirit of the present invention, therefore, the scope of the present invention in teachings of the present invention and announcement The content disclosed in embodiment should be not limited to, and various replacements and modification without departing substantially from the present invention should be included, and is this patent Shen Please claim covered.

Claims (8)

1. a kind of Chinese medicine composition for treating the protrasion of the lumbar intervertebral disci, it is characterised in that the Chinese medicine composition includes：The raw Radix Astragali 30-60g, Poria cocos 10-20g, umbellate pore furgus 10-20g, root of Chinese clematis 15-20g, pawpaw 12-20g, dried orange peel 6-12g, RADIX ANGELICAE SINEINSIS 15-20g, Ligusticum wallichii 20-30g, boiling hot leech 9-12g, earthworm 9-12g, Semen Coicis Semen Coicis 15-30g, root of fangji 15-30g, semen brassicae 6-9g, rhizoma atractylodis macrocephalae 10-20g, radix cyathulae 10-20g.
2. Chinese medicine composition according to claim 1, it is characterised in that the Chinese medicine composition includes：Raw Radix Astragali 40- 50g, Poria cocos 15-20g, umbellate pore furgus 15-20g, root of Chinese clematis 15-18g, pawpaw 15-20g, dried orange peel 10-12g, RADIX ANGELICAE SINEINSIS 15-20g, river Rhizome of chuanxiong 20-30g, boiling hot leech 10-12g, earthworm 10-12g, Semen Coicis Semen Coicis 20-30g, root of fangji 20-30g, semen brassicae 6-8g, rhizoma atractylodis macrocephalae 15-20g, radix cyathulae 15-20g.
3. Chinese medicine composition according to claim 1 or 2, it is characterised in that the Chinese medicine composition includes：The raw Radix Astragali 45g, Poria cocos 18g, umbellate pore furgus 16g, root of Chinese clematis 16g, pawpaw 18g, dried orange peel 11g, RADIX ANGELICAE SINEINSIS 17g, Ligusticum wallichii 25g, scald leech 11g, Imperial 10g, Semen Coicis Semen Coicis 26g, root of fangji 24g, semen brassicae 7g, rhizoma atractylodis macrocephalae 16g, radix cyathulae 18g.
4. Chinese medicine composition according to claim 1 or 2, it is characterised in that the medicament that the Chinese medicine composition is made is Chinese herbs decoction.
5. Chinese medicine composition according to claim 1 or 2, it is characterised in that the medicament that the Chinese medicine composition is made is Mixture.
6. the method that Chinese medicine composition described in a kind of use Claims 1 to 5 any one prepares medicament, it is characterised in that bag Include：
S1, a course for the treatment of amount is weighed by the part by weight of each raw material of the Chinese medicine composition, by the Chinese medicine of one course for the treatment of amount Composition is equally divided into two parts, and portion adds water 10~20 times of amounts, another 5~9 times of amount that add water；
S2, add water two parts of Chinese medicine compositions are decocted at least one times respectively, filtrate is filtrated to get after decoction, and two parts of filtrates are closed And, and the filtrate after merging is concentrated, it is concentrated into concentrate of the concentration range equivalent to 1~10g/ml crude drugs；
S3, the concentrate is added 2~6 times of amount alcohol contents be 60% ethanol, refrigeration stands 20~30h, filtration, to ethanol without Filtrate is reclaimed after alcohol taste；
S4, boils the recovery filtrate, plus 2~6 grams of sodium benzoate, 0.2~0.4 gram of nipagin ethylester, filtration is let cool, and is added water to 400~600ml, it is filling while hot.
7. the preparation method of medicament according to claim 6, it is characterised in that including：
S1 ', a course for the treatment of amount is weighed by the part by weight of each raw material of the Chinese medicine composition, by the Chinese medicine of one course for the treatment of amount Composition is equally divided into two parts, and portion adds water 10 times and measured, and another adds water 8 times and measured；
S2 ', add water two parts of Chinese medicine compositions are decocted twice respectively, filtrate is filtrated to get after decoction, and two parts of filtrates are merged, And concentrate the filtrate after merging, it is concentrated into concentrate of the concentration range equivalent to 5g/ml crude drugs；
S3 ', by the concentrate add 3 times amount alcohol contents be 60% ethanol, refrigeration stand 24h, filtration, to ethanol without alcohol taste after Filtrate is reclaimed；
S4 ', boils the recovery filtrate, plus 3 grams of sodium benzoate, 0.25 gram of nipagin ethylester, filtration is let cool, and adds water to 500ml, It is filling while hot.
8. the preparation method of the medicament according to claim 6 or 7, it is characterised in that in step S1, one course for the treatment of amount For 7 days.
CN201710682994.3A 2017-08-10 2017-08-10 Treat the Chinese medicine composition of the protrasion of the lumbar intervertebral disci and its preparation method of medicament Pending CN107281417A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115444904A (en) * 2022-10-26 2022-12-09 湖南祥民制药有限公司 Compound notoginseng capsule for treating protrusion of intervertebral disc

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115444904A (en) * 2022-10-26 2022-12-09 湖南祥民制药有限公司 Compound notoginseng capsule for treating protrusion of intervertebral disc

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