CN107279075A - A kind of silkworm in spring egg hatching method - Google Patents
A kind of silkworm in spring egg hatching method Download PDFInfo
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- CN107279075A CN107279075A CN201710508101.3A CN201710508101A CN107279075A CN 107279075 A CN107279075 A CN 107279075A CN 201710508101 A CN201710508101 A CN 201710508101A CN 107279075 A CN107279075 A CN 107279075A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
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Abstract
The present invention relates to a kind of silkworm in spring egg hatching method, following steps are specifically included：（1）Silkworm seed is pre-processed；（2）The preservation of silkworm seed；（3）The pickling processing of silkworm seed；（4）The hatching of silkworm seed.By experiment, the hatching of silkworm in spring ovum is carried out using the inventive method, the hatching success of silkworm seed is promoted to 65% by the 40% of existing method, and effect is notable, while the survival rate of newly-hatched silkworm is also significantly improved, up to 95%, there is important enlightenment to silkworm aquaculture technology innovation.
Description
Technical field
The invention belongs to cultured insect technical field, and in particular to a kind of silkworm in spring egg hatching method.
Background technology
Silkworm is bred with ovum.Silkworm seed is looked like particulate sesame, wide about 1 millimeter, thick about 0.5 millimeter.One female moth is about
450-500 silkworm seeds can be produced, 1 gram is weighed about.It is faint yellow or yellow when silkworm seed is just given birth to, red bean color was changed into through 1-2 days, then through 3-
It is changed into celadon or purple again after 4 days, just no longer changes, referred to as fixes color.Silkworm seed outer layer is hard chorion, and the inside is
Embryo in yolk and serous coat, embryonated egg is constantly absorbed nourishment in growth course, gradually develops into newly-hatched silkworm, and it is climbed from chorion
Out, become white or faint yellow after chorion is empty.
During egg-incubation, temperature, humidity are considered, while environment of hatching is carried out disinfection, otherwise egg-incubation
Success rate is low, newly-hatched silkworm survival rate is low.
The content of the invention
In order to solve the above problems, the invention provides a kind of silkworm in spring egg hatching method.
The present invention is achieved through the following technical solutions：
A kind of silkworm in spring egg hatching method, specifically includes following steps：
（1）Silkworm seed is pre-processed
15-16 hours after silkworm moth lays eggs, silkworm seed is connected into paper and is used together clear water immersion, after after paper maceration, lower silkworm is gently washed by rubbing with the hands
Ovum, will use 40-45 DEG C of solution to soak 10-15 minutes, then be transferred to 2-4 DEG C of deepfreeze processing in refrigerating chamber, can effectively kill
Go out silkworm seed surface germ, silkworm seed embryo viability is not destroyed, contributes to the follow-up chilling treatment of silkworm seed, it is ensured that silkworm seed subsequently hatches success
Rate；The solution, is specifically comprised the following steps:
A according to the mass fraction, takes the peroxide that 100-120 parts of sodium bicarbonate aqueous solution, mass fraction that mass fraction is 5% are 15%
Change obtained solution A after 10-12 parts of aqueous solution of hydrogen, 1-2 parts of sodium chloride, 2-3 parts of part mixing of glucose；
B according to the mass fraction, takes 5-8 parts of dried peppermint leaf, 15-20 parts of folium artemisiae argyi, 3-5 parts of Dalmatian chrysanthemum, 5-8 parts of toad grass, purslane
1-3 parts of cleaning and choppings, the 200-220 parts of infusions of adding water are filtered after 15-20 minutes, obtain filtrate and filter residue, then by the juice in filter residue
Liquid is fully extruded to be merged with the filtrate, obtains solution B；
C is by the solution A, solution B in mass ratio 1:After 1 uniform mixing, the solution is produced；
（2）The preservation of silkworm seed
The silkworm seed handled well is air-dried after surface moisture, 2-4 DEG C is placed in after sealing, ventilation, is stored in light protected environment, can effectively be protected
The bioactivity of silkworm seed is demonstrate,proved, while reducing the rate of development of silkworm seed, it is effectively developed embryo after being taken out at the beginning of spring, carries
High Hatching Rate of Silkworm Eggs；
（3）The pickling processing of silkworm seed
After the beginning of spring, the silkworm seed of refrigeration is taken out, the use of 35-40 DEG C of mass fraction is 2% after first being rinsed 30-45 seconds using clear water
Sodium bicarbonate aqueous solution immersion treatment 5-8 minutes, helps to stimulate silkworm seed to adapt to environment temperature, convenient follow-up promotion silkworm seed continues
The processing of development；Then soak silkworm seed 8-10 minutes using 25 DEG C of decoctions, greatly shortened than the conventional pickling time, effective stimulus silkworm
Ovum stops its diapause process, promotes silkworm seed to continue to develop, while the shorter pickling time helps to reduce pickling technical difficulty, keeps away
Exempting from pickling overlong time causes silkworm seed to lose hatchability；The decoction, is specifically worth by following steps:
A according to the mass fraction, takes 100-120 parts of hydrochloric acid solution, 0.1-0.3 parts of magnesium chloride, iron chloride that mass fraction is 18%
Obtained solution A after 0.2-0.4 parts of uniform mixing；
B according to the mass fraction, is crushed to 120 mesh after taking 1-2 parts of the red sage root, 8-12 parts of the bark of official magnolia, 10-15 parts of stir-frying of pumpkin seeds, added water
Filtrate heating is concentrated into 1/5th of original volume, obtains solution B by 100-120 parts of refluxing extractions 60-65 minutes, filtering and impurity removing；
C is by the solution A, solution B in mass ratio 3:The decoction is produced after 1 mixing；
（4）The hatching of silkworm seed
It will be air-dried after the silkworm seed depickling handled well, you can hatched, 7 days point green grass or young crops is hatched successfully after 10 days.
Beneficial effects of the present invention：The silkworm seed that the solution immersion has just been given birth to, then deepfreeze processing, can effectively be killed
Silkworm seed surface germ, while not destroying silkworm seed embryo viability, abolishes silkworm seed diapause process, contributes to the preservation of silkworm seed, it is ensured that silkworm
The follow-up hatching success of ovum；The silkworm seed handled well is placed in low temperature, ventilation, light protected environment and stored, silkworm seed can be effectively ensured
Bioactivity, while reducing the rate of development of silkworm seed, reduces the consumption of nutritional ingredient inside silkworm seed, makes after it takes out at the beginning of spring
There can be enough nutrients to develop into embryo, further improve Hatching Rate of Silkworm Eggs；Hatch the pickling processing before silkworm seed, than conventional pickling
Time greatly shortens, and effective stimulus silkworm seed stops its diapause process, promotes silkworm seed to continue to develop, while the shorter pickling time has
Help reduce pickling technical difficulty, it is to avoid pickling overlong time causes silkworm seed to lose hatchability.By experiment, the present invention is used
Method carries out the hatching of silkworm in spring ovum, and the hatching success of silkworm seed is promoted to 65% by the 40% of existing method, and effect is notable, while silkworm
The survival rate of ant is also significantly improved, and up to 95%, there is important enlightenment to silkworm aquaculture technology innovation.
Embodiment
Embodiment 1
A kind of silkworm in spring egg hatching method, specifically includes following steps：
（1）Silkworm seed is pre-processed
15 hours after silkworm moth lays eggs, silkworm seed is connected into paper and is used together clear water immersion, after after paper maceration, lower silkworm seed is gently washed by rubbing with the hands,
40 DEG C of solution will be used to soak 10 minutes, 2 DEG C of deepfreeze processing in refrigerating chamber are then transferred to, can effectively kill silkworm seed surface disease
Bacterium, silkworm seed embryo viability is not destroyed, contributes to the follow-up chilling treatment of silkworm seed, it is ensured that the follow-up hatching success of silkworm seed；The solution,
Specifically comprise the following steps:
A according to the mass fraction, takes the hydrogen peroxide that 100 parts of sodium bicarbonate aqueous solution, mass fraction that mass fraction is 5% are 15%
Obtained solution A after 10 parts of the aqueous solution, 1 part of sodium chloride, 2 parts of part mixing of glucose；
B according to the mass fraction, is taken after 5 parts of dried peppermint leaf, 15 parts of folium artemisiae argyi, 3 parts of Dalmatian chrysanthemum, 5 parts of toad grass, 1 part of purslane clean
Chopping, the 200 parts of infusions of adding water are filtered after 15 minutes, obtain filtrate and filter residue, then the juice in filter residue is fully extruded and the filter
Liquid merges, and obtains solution B；
C is by the solution A, solution B in mass ratio 1:After 1 uniform mixing, the solution is produced；
（2）The preservation of silkworm seed
The silkworm seed handled well is air-dried after surface moisture, 2 DEG C are placed in after sealing, ventilation, is stored in light protected environment, can be effectively ensured
The bioactivity of silkworm seed, while reducing the rate of development of silkworm seed, makes it effectively to develop embryo after being taken out at the beginning of spring, improves
Hatching Rate of Silkworm Eggs；
（3）The pickling processing of silkworm seed
After the beginning of spring, the silkworm seed of refrigeration is taken out, after first being rinsed 30 seconds using clear water, the bicarbonate that 35 DEG C of mass fractions are 2% is used
Sodium water solution immersion treatment 5 minutes, helps to stimulate silkworm seed to adapt to environment temperature, convenient follow-up promotion silkworm seed continues the place developed
Reason；Then soak silkworm seed 8 minutes using 25 DEG C of decoctions, greatly shortened than the conventional pickling time, it is stagnant that effective stimulus silkworm seed stops its
Process is educated, promotes silkworm seed to continue to develop, while the shorter pickling time helps to reduce pickling technical difficulty, it is to avoid the pickling time
It is long to cause silkworm seed to lose hatchability；The decoction, is specifically worth by following steps:
A according to the mass fraction, takes 100 parts of hydrochloric acid solution, 0.1 part of magnesium chloride, 0.2 part of iron chloride that mass fraction is 18% uniform
Obtained solution A after mixing；
B according to the mass fraction, is crushed to 120 mesh after taking 1 part of the red sage root, 8 parts of the bark of official magnolia, 10 parts of stir-frying of pumpkin seeds, adds water 100 parts and flow back
Extract 60-65 minutes, filtering and impurity removing, filtrate heating is concentrated into 1/5th of original volume, solution B is obtained；
C is by the solution A, solution B in mass ratio 3:The decoction is produced after 1 mixing；
（4）The hatching of silkworm seed
It will be air-dried after the silkworm seed depickling handled well, you can hatched, 7 days point green grass or young crops is hatched successfully after 10 days.
Embodiment 2
A kind of silkworm in spring egg hatching method, specifically includes following steps：
（1）Silkworm seed is pre-processed
16 hours after silkworm moth lays eggs, silkworm seed is connected into paper and is used together clear water immersion, after after paper maceration, lower silkworm seed is gently washed by rubbing with the hands,
45 DEG C of solution will be used to soak 15 minutes, 4 DEG C of deepfreeze processing in refrigerating chamber are then transferred to, can effectively kill silkworm seed surface disease
Bacterium, silkworm seed embryo viability is not destroyed, contributes to the follow-up chilling treatment of silkworm seed, it is ensured that the follow-up hatching success of silkworm seed；The solution,
Specifically comprise the following steps:
A according to the mass fraction, takes the hydrogen peroxide that 120 parts of sodium bicarbonate aqueous solution, mass fraction that mass fraction is 5% are 15%
Obtained solution A after 12 parts of the aqueous solution, 2 parts of sodium chloride, 3 parts of part mixing of glucose；
B according to the mass fraction, is taken after 8 parts of dried peppermint leaf, 20 parts of folium artemisiae argyi, 5 parts of Dalmatian chrysanthemum, 8 parts of toad grass, 3 parts of purslane clean
Chopping, the 220 parts of infusions of adding water are filtered after 20 minutes, obtain filtrate and filter residue, then the juice in filter residue is fully extruded and the filter
Liquid merges, and obtains solution B；
C is by the solution A, solution B in mass ratio 1:After 1 uniform mixing, the solution is produced；
（2）The preservation of silkworm seed
The silkworm seed handled well is air-dried after surface moisture, 4 DEG C are placed in after sealing, ventilation, is stored in light protected environment, can be effectively ensured
The bioactivity of silkworm seed, while reducing the rate of development of silkworm seed, makes it effectively to develop embryo after being taken out at the beginning of spring, improves
Hatching Rate of Silkworm Eggs；
（3）The pickling processing of silkworm seed
After the beginning of spring, the silkworm seed of refrigeration is taken out, after first being rinsed 45 seconds using clear water, the bicarbonate that 40 DEG C of mass fractions are 2% is used
Sodium water solution immersion treatment 8 minutes, helps to stimulate silkworm seed to adapt to environment temperature, convenient follow-up promotion silkworm seed continues the place developed
Reason；Then soak silkworm seed 10 minutes using 25 DEG C of decoctions, greatly shortened than the conventional pickling time, it is stagnant that effective stimulus silkworm seed stops its
Process is educated, promotes silkworm seed to continue to develop, while the shorter pickling time helps to reduce pickling technical difficulty, it is to avoid the pickling time
It is long to cause silkworm seed to lose hatchability；The decoction, is specifically worth by following steps:
A according to the mass fraction, takes 120 parts of hydrochloric acid solution, 0.3 part of magnesium chloride, 0.4 part of iron chloride that mass fraction is 18% uniform
Obtained solution A after mixing；
B according to the mass fraction, 120 mesh is crushed to after taking 2 parts of the red sage root, 12 parts of the bark of official magnolia, 15 parts of stir-frying of pumpkin seeds, is added water 120 parts and is returned
Stream is extracted 65 minutes, filtering and impurity removing, and filtrate heating is concentrated into 1/5th of original volume, solution B is obtained；
C is by the solution A, solution B in mass ratio 3:The decoction is produced after 1 mixing；
（4）The hatching of silkworm seed
It will be air-dried after the silkworm seed depickling handled well, you can hatched, 7 days point green grass or young crops is hatched successfully after 10 days.
Comparative example 1
This comparative example 1 compared with Example 1, without step（1）The silkworm seed pretreatment operation, method step in addition
Rapid all same.
Comparative example 2
This comparative example 2 compared with Example 2, without using step（3）The pickling processing method, only with conventional pickling side
Method, method and step all same in addition.
Control group
Existing silkworm in spring egg hatching method.
Respectively hatching success and the nursing of silkworm seed are observed using the processing of above-mentioned 5 kinds of methods with a batch of silkworm in spring ovum
The survival rate of newly-hatched silkworm, many experiments, statistical result such as table 1 two days later:
Table 1
| Hatching success（%） | Newly-hatched silkworm survival rate two days later（%） | |
| Embodiment 1 | 65 | 95 |
| Embodiment 2 | 65 | 95 |
| Comparative example 1 | 45 | 85 |
| Comparative example 2 | 55 | 80 |
| Control group | 40 | 80 |
As shown in Table 1, the hatching of silkworm in spring ovum is carried out using the inventive method, the hatching success of silkworm seed is by the 40% of existing method
65% is promoted to, effect is notable, while the survival rate of newly-hatched silkworm is also significantly improved, up to 95%, have to the technology innovation of silkworm aquaculture
There is important enlightenment.
Claims (3)
1. a kind of silkworm in spring egg hatching method, it is characterised in that specifically include following steps：
（1）Silkworm seed is pre-processed
15-16 hours after silkworm moth lays eggs, silkworm seed is connected into paper and is used together clear water immersion, after after paper maceration, lower silkworm is gently washed by rubbing with the hands
Ovum, will use 40-45 DEG C of solution to soak 10-15 minutes, then be transferred to 2-4 DEG C of deepfreeze processing in refrigerating chamber；The solution,
Specifically comprise the following steps:
A according to the mass fraction, takes the peroxide that 100-120 parts of sodium bicarbonate aqueous solution, mass fraction that mass fraction is 5% are 15%
Change obtained solution A after 10-12 parts of aqueous solution of hydrogen, 1-2 parts of sodium chloride, 2-3 parts of part mixing of glucose；
B according to the mass fraction, takes 5-8 parts of dried peppermint leaf, 15-20 parts of folium artemisiae argyi, 3-5 parts of Dalmatian chrysanthemum, 5-8 parts of toad grass, purslane
1-3 parts of cleaning and choppings, the 200-220 parts of infusions of adding water are filtered after 15-20 minutes, obtain filtrate and filter residue, then by the juice in filter residue
Liquid is fully extruded to be merged with the filtrate, obtains solution B；
C is by the solution A, solution B in mass ratio 1:After 1 uniform mixing, the solution is produced；
（2）The preservation of silkworm seed
The silkworm seed handled well is air-dried after surface moisture, 2-4 DEG C is placed in after sealing, ventilation, is stored in light protected environment；
（3）The pickling processing of silkworm seed
After the beginning of spring, the silkworm seed of refrigeration is taken out, the use of 35-40 DEG C of mass fraction is 2% after first being rinsed 30-45 seconds using clear water
Sodium bicarbonate aqueous solution immersion treatment 5-8 minutes, then soaks silkworm seed 8-10 minutes using 25 DEG C of decoctions；The decoction, specifically
It is worth by following steps:
A according to the mass fraction, takes 100-120 parts of hydrochloric acid solution, 0.1-0.3 parts of magnesium chloride, iron chloride that mass fraction is 18%
Obtained solution A after 0.2-0.4 parts of uniform mixing；
B according to the mass fraction, is crushed to 120 mesh after taking 1-2 parts of the red sage root, 8-12 parts of the bark of official magnolia, 10-15 parts of stir-frying of pumpkin seeds, added water
Filtrate heating is concentrated into 1/5th of original volume, obtains solution B by 100-120 parts of refluxing extractions 60-65 minutes, filtering and impurity removing；
C is by the solution A, solution B in mass ratio 3:The decoction is produced after 1 mixing；
（4）The hatching of silkworm seed
It will be air-dried after the silkworm seed depickling handled well, you can hatched, 7 days point green grass or young crops is hatched successfully after 10 days.
2. a kind of silkworm in spring egg hatching method according to claim 1, it is characterised in that step（1）The solution, specifically by
Following method is made:
A according to the mass fraction, takes the hydrogen peroxide that 120 parts of sodium bicarbonate aqueous solution, mass fraction that mass fraction is 5% are 15%
Obtained solution A after 12 parts of the aqueous solution, 2 parts of sodium chloride, 3 parts of part mixing of glucose；
B according to the mass fraction, is taken after 8 parts of dried peppermint leaf, 20 parts of folium artemisiae argyi, 5 parts of Dalmatian chrysanthemum, 8 parts of toad grass, 3 parts of purslane clean
Chopping, the 220 parts of infusions of adding water are filtered after 20 minutes, obtain filtrate and filter residue, then the juice in filter residue is fully extruded and the filter
Liquid merges, and obtains solution B；
C is by the solution A, solution B in mass ratio 1:After 1 uniform mixing, the solution is produced.
3. a kind of silkworm in spring egg hatching method according to claim 1, it is characterised in that step（3）The decoction, specifically by
Following steps are worth:
A according to the mass fraction, takes 120 parts of hydrochloric acid solution, 0.3 part of magnesium chloride, 0.4 part of iron chloride that mass fraction is 18% uniform
Obtained solution A after mixing；
B according to the mass fraction, 120 mesh is crushed to after taking 2 parts of the red sage root, 12 parts of the bark of official magnolia, 15 parts of stir-frying of pumpkin seeds, is added water 120 parts and is returned
Stream is extracted 65 minutes, filtering and impurity removing, and filtrate heating is concentrated into 1/5th of original volume, solution B is obtained；
C is by the solution A, solution B in mass ratio 3:The decoction is produced after 1 mixing.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109588376A (en) * | 2018-12-27 | 2019-04-09 | 广西融安县金鼎制丝有限责任公司 | A kind of store method of silkworm seed |
| CN109588377A (en) * | 2018-12-27 | 2019-04-09 | 广西融安县金鼎制丝有限责任公司 | A kind of egg-incubation method |
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| JP2012044889A (en) * | 2010-08-25 | 2012-03-08 | Nihon Univ | Method of artificially incubating dormant egg of silk worm by dimethyl sulfoxide (dmso), and method of introducing chemical material into egg |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109588376A (en) * | 2018-12-27 | 2019-04-09 | 广西融安县金鼎制丝有限责任公司 | A kind of store method of silkworm seed |
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Application publication date: 20171024 |