CN101120659B - Method for treating and preventing scuticociliatosis in fishes - Google Patents
Method for treating and preventing scuticociliatosis in fishes Download PDFInfo
- Publication number
- CN101120659B CN101120659B CN2007100038691A CN200710003869A CN101120659B CN 101120659 B CN101120659 B CN 101120659B CN 2007100038691 A CN2007100038691 A CN 2007100038691A CN 200710003869 A CN200710003869 A CN 200710003869A CN 101120659 B CN101120659 B CN 101120659B
- Authority
- CN
- China
- Prior art keywords
- fish
- shield
- fine
- infection disease
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/20—Elemental chlorine; Inorganic compounds releasing chlorine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/08—Alkali metal chlorides; Alkaline earth metal chlorides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/40—Peroxides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Inorganic Chemistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Farming Of Fish And Shellfish (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The present invention provides a method for treating and preventing scuticociliatosis in breeding fishes. Adding stabilized chlorine dioxide or chlorite, organic carboxylic acid and hydrogen peroxide into a cistern which is closed over ultraviolet, and make the concentration of them be above 0.5ppm and below 10ppm, above 0.015ppm and below 0.3ppm, above 5ppm and below 30ppm separately, and to implement drug bath to fish in the cistern. By means of the three medicament combination, it is easy to treat scuticociliatosis in breeding fishes. In addition, due to killing the shield infusorian, thus to prevent scuticociliatosis from occurring without blight to fishes.
Description
Technical field
The present invention relates to use stabilizing chlorine dioxide or chlorite, organic carboxyl acid and hydrogen peroxide, treatment or prevention are owing to the method for the fine worm disease of shield of the parasitic fish that occur of the fine worm of shield (scuticociliate).
Background technology
Because the development of the cultural technique of seafood fish, senior fishes such as flatfish, porgy, filefish have become the breed object in recent years.In the plant of these senior fishes, pre-anti-virus, pathogenic bacteria, the caused disease of epizoon are very important for keeping growth, the raising output of culturing fish.
Particularly, parasitism has epizoon in the fish if culture, though can not cause big disaster at parasitic number after a little while, but in case arrive May～July (22 ℃～26 ℃ of water temperatures) that the water temperature phase is fitted in breeding, the egg hatch adult, and lay eggs in a large number simultaneously, the mortality of fish takes place to culture easily.
In addition, when being unlikely to death even infection has parasite, owing to parasitic reason, the physique of culturing fish weakens, and is subjected to other bacterial infection easily, and it is bad perhaps to ingest, and causes the loss of commodity value easily.
As the insect-repelling method or the infection mitigation method of culturing fish parasites, for example in patent documentation 1, disclose by forulic acid and lactic acid combination are added in the raising water, can be from being driven away parasite the fish of Trichodina (Trichodina), skin fluke (skin fluke) or genus Pseudodactylogrus from Anguilla anguilla parasitic infections such as (Pseudodactylogyrus).
In addition, in patent documentation 2, disclose by throwing and be the parasite inhibitor of active ingredient the method that suppresses and prevent the parasite disease of mariculture fish with cocoa bean composition (skin of beancurd of cocoa power or cocoa bean).
In addition, in patent documentation 3, disclose and in feed or pond, added δ-glycyl pyruvic acid, treated by the method for the fish of pathogenic microbes and parasitic infection.
[patent documentation 1] Japan Patent spy opens the 2006-77000 communique
[patent documentation 2] Japan Patent spy opens the 2006-61107 communique
[patent documentation 3] Japan Patent spy opens the 2001-316255 communique
Summary of the invention
In the infectious diseases of the albacore that is caused by parasite, the infection disease (the fine insect infection disease (scuticociliatosis) of shield) of the flatfish juvenile fish that is caused by the fine worm of shield is popular in the seedling grown place and the plant of Japanese various places.As the fine worm of the shield of pathogene is foreign pyriform, and length is 30～45 μ m, along major axis 8～12 fine hair row is arranged, and has 1 fine hair at tail end.Body colour bleaches in case the fine worm of shield is invaded body surface or fin, and is visible rotten to the corn and hemorrhage.Also can invade in addition to squama Nang, subepidermal connective tissue and brain.
Like this, invading brain is the feature of the fine worm of shield, if because affected part only limits to brain, then be attended by the outward appearance symptom hardly, does not determine also that sometimes reason breed fish is with regard to mortality with.Its process of invading the fish body is not clear, owing to deeply invade to the fish body, therefore different with other parasitosis, can not come expelling parasite by dipping.
In patent documentation 3, in vivo and the effect (paragraph [0064] and [0066]) that infects of the external fish body of having confirmed the fine worm of pre-shield.Though put down in writing when in the pond of suffering from the fine insect infection disease flatfish juvenile fish of shield (severe fish), adding δ-glycyl pyruvic acid, the vigor of fish improves, dead fish significantly reduces, and does not detect the fine worm (paragraph [0069]) of shield, still discloses concrete numerical value such as sample number.
For the fine insect infection disease of shield, also do not develop effective cure at present.In addition, habitual always in the past formalin is used to culture fish by complete prohibition, in case therefore the fine insect infection disease of shield is taken place in plant, the death of a large amount of breed fish will be arranged unavoidably then, and plant manages and will be hard hit.
The objective of the invention is to, the method for the fine insect infection disease of shield of the breed fish that treatment and prevention do not have the effective countermeasure method in the past is provided.
The inventor finds, by add stabilizing chlorine dioxide or chlorite, organic carboxyl acid and hydrogen peroxide in breeding water, can treat the breed fish that suffers from the fine insect infection disease of shield, so finished the present invention.
Specifically, the present invention relates to a kind of method, it is the methods of treatment of the fine insect infection disease of shield of fish, it is characterized in that, at the stabilizing chlorine dioxide or the chlorite that contain respectively more than the 0.5ppm, below the 10ppm, the organic carboxyl acid that 0.015ppm is above, 0.3ppm is following is in the soup of the hydrogen peroxide that 5ppm is above, 30ppm is following, covering under the ultraviolet state, making fish carry out dipping.
Compare with independent use chlorine dioxide, by stabilizing chlorine dioxide or chlorite, organic carboxyl acid and hydrogen peroxide are also used, can kill the fine worm of shield of invading to the fish body in vivo for lower concentration with far away, can not educate sashimi (raw fish) and bring harmful effect ground that the fine insect infection disease of shield is effectively treated.
In addition, the present invention relates to a kind of method, it is the methods of treatment of the fine insect infection disease of shield of fish, it is characterized in that, at the stabilizing chlorine dioxide or the chlorite that contain respectively more than the 0.5ppm, below the 10ppm, the organic carboxyl acid that 0.015ppm is above, 0.3ppm is following is in the soup of the hydrogen peroxide that 5ppm is above, 30ppm is following, covering under the ultraviolet state, making fish carry out dipping.
Compare with independent use chlorine dioxide or chlorite, by stabilizing chlorine dioxide or chlorite, organic carboxyl acid and hydrogen peroxide are also used, can with far be lower concentration, at short notice killing in vitro shield fibre worm, therefore can carry out desinsection to adopt the fine worm of the shield that exists in the seawater of water as breed water, also can prevent to occur in the pond the fine insect infection disease of shield.Also can prevent to infect from the fish that suffers from the fine insect infection disease of shield in the water layer infection diffusion of healthy fish in addition.
Fish as the object of fine insect infection disease methods of treatment of shield of the present invention and the fine insect infection disease of shield prevention method is that the fish that suffers from the fine insect infection disease of shield gets final product, there is no particular limitation to its kind, particularly preferably the flatfish of often being cultured in the albacore, turbot (Turbot) or filefish.
The organic carboxyl acid that uses among the present invention is preferably the combination more than a kind or 2 kinds that is selected from acetic acid, citric acid, malic acid and succinic acid.This is because these organic carboxyl acids do not have toxicity and are weak acid, therefore is difficult to make the pH in pond to change, and fish is not had dysgenic cause.
The time of dipping was preferably every day more than 4 hours.
By methods of treatment of the present invention and prevention method, can carry out the treatment and the prevention of efficient and cost-effective to the fine insect infection disease of the shield in the plant.
With reference to the accompanying drawings, by the detailed description of following preferred forms, come clear and definite above-mentioned purpose of the present invention, other purpose, feature and advantage.
Description of drawings
The diagrammatic sketch of the variation of the death toll of flatfish juvenile fish in the plant of [Fig. 1] demonstration comparative example.
The diagrammatic sketch of the variation of the death toll of flatfish juvenile fish in the plant of [Fig. 2] demonstration embodiment 1.
The diagrammatic sketch of the variation of the death toll of flatfish juvenile fish in the plant of [Fig. 3] demonstration embodiment 2.
The diagrammatic sketch of the variation of the death toll of flatfish juvenile fish in the plant of [Fig. 4] demonstration embodiment 3.
Embodiment
Below, the accompanying drawing with reference to suitable describes embodiments of the present invention, and in addition, the present invention is not limited only to this.
(preliminary experiment)
By experiment external, confirm formalin, chlorophos (Trichlorfon) and stabilizing chlorine dioxide insecticidal effect to the fine worm of shield.At first, take the fine worm of shield from the flatfish that suffers from the fine insect infection disease of shield, then in the artificial seawater of the sliced meat that are added with the flatfish that has homogenized in incubated at room temperature 24 hours.The concentration of the fine worm of shield is about 30/mL in the culture fluid.
In culture dish, pick and place the above-mentioned three kinds medicament 20mL that is diluted to each concentration with the seawater of sterilization after filtration, add the fine worm culture fluid of shield 1mL again.Observe the activity of the fine worm of shield afterwards at microscopically (40 times of multiplication factors), if stop action fully then be judged to be death.Show the result in table 1.In addition, formalin is that JIS special grade chemical, chlorophos are that the system マ デ ソ of バ ィ ェ Le Co., Ltd. Application 20%, stabilizing chlorine dioxide are for helping river chemistry system PVC オ ト one Network (chlorine dioxide concentration is 5%).
Table 1
For the insecticidal effect in the table 1, after the fine worm of shield is being added medicament 3 days be expressed as " zero " when dead with interior, when 3 days with interior be expressed as when not having death " * ".When using formalin or chlorophos, can not carry out desinsection to the fine worm of shield, but when being to use stabilizing chlorine dioxide, after 3 days, the fine worm of the shield in the culture dish all can be killed with the concentration of 10ppm even concentration is 10ppm.
Then, improve the concentration of stabilizing chlorine dioxide, determine that it can kill the fine worm of shield at short notice.Experimental technique is same as described above.Show the result in table 2.
Table 2
| The concentration of stabilizing chlorine dioxide (ppm) | The time that desinsection needs |
| 10 | 3 |
| 100 | 5 |
| 500 | 2 hours |
| 1000 | About 5 minutes |
Stabilizing chlorine dioxide concentration is high more, and it is short more to kill the required time of the fine worm of shield.But having confirmed needs more than the 100ppm in order to carry out desinsection in a few hours, particularly for desinsection in 1 hour, needs the high concentration of hundreds of ppm.Therefore,, be difficult to bring under the dysgenic prerequisite, in the short time, kill the fine worm of shield, be difficult to the fine insect infection disease of pre-shield in raising not to fish if use stabilizing chlorine dioxide separately.
Therefore, in order to improve insecticidal effect, attempt stabilizing chlorine dioxide and a kind of citric acid and usefulness as organic carboxyl acid are carried out and above-mentioned same experiment to the fine worm of shield.The concentration rate of chlorine dioxide and citric acid is 10: 1 at this.It is the results are shown in table 3.
Table 3
| The concentration of stabilizing chlorine dioxide (ppm) | Citric acid concentration (ppm) | Insecticidal effect | The time that desinsection needs |
| 0.1 | 0.01 | × | |
| 1 | 0.1 | × | |
| 5 | 0.5 | × | |
| 10 | 1 | ○ | 3 |
| 20 | 2 | ○ | About 30 |
| 30 | 3 | ○ | About 20 |
| 40 | 4 | ○ | About 20 |
| 50 | 5 | ○ | About 5 minutes |
By also using citric acid, if stabilizing chlorine dioxide concentration more than 20ppm, can all be killed the fine worm of shield with interior at 1 hour.The concentration rate of stabilizing chlorine dioxide and citric acid be 10: 0.5～10: 2 also obtain same experimental result.In addition, use acetic acid, malic acid, succinic acid, tartaric situation also to obtain the experimental result identical as organic carboxyl acid with citric acid.
In addition, in order to improve insecticidal effect, attempt stabilizing chlorine dioxide, citric acid, three kinds of materials of hydrogen peroxide and usefulness are carried out experiment same as described above to the fine worm of shield.The concentration rate of stabilizing chlorine dioxide, citric acid and hydrogen peroxide is 100: 0.05: 5 at this.Show the result in table 4.
Table 4
| The concentration of stabilizing chlorine dioxide (ppm) | Citric acid concentration (ppm) | H 2O 2Concentration (ppm) | Insecticidal effect | The time that desinsection needs |
| 0.1 | 5×10 5 | 5×10 -3 | × | |
| 0.2 | 1×10 -4 | 1×10 -2 | ○ | About 2 hours |
| 0.5 | 2.5×10 4 | 2.5×10 2 | ○ | About 40 minutes |
| 1 | 5×10 4 | 5×10 2 | ○ | About 20 minutes |
| 5 | 2.5×10 3 | 2.5×10 -1 | ○ | About 2 |
| 10 | 5×10 3 | 5×10 1 | ○ | ＞1 |
| 20 | 1×10 2 | 1 | ○ | ＞1 minute |
By also using citric acid and hydrogen peroxide, if stabilizing chlorine dioxide concentration just can all killed the fine worm of shield within 1 hour more than the 0.5ppm.The concentration rate of stabilizing chlorine dioxide, citric acid and hydrogen peroxide be 100: 0.01: 1～100: 2: 20 also obtain same experimental result.Use acetic acid, malic acid, succinic acid, tartaric situation also to obtain the experimental result identical as organic carboxyl acid in addition with citric acid.
In addition, use as chloritic a kind of sodium chlorite (NaClO with same concentrations
2) when replacing stabilizing chlorine dioxide, also obtain experimental result same as described above.
[comparative example]
(disaster of the plant that causes by the fine insect infection disease of shield)
At this, in Fig. 1, shown the fine insect infection disease of the shield disaster that juvenile fish is taken place in the flatfish plant.In this plant, according to juvenile fish 2500 tails below the long 20cm of the breed body of the condition shown in the table 5.
Table 5
| Pool size | 7.5m×8m |
| Water level | 50cm |
| |
20～22℃ |
| The water yield | 30t |
| Rate of |
1～1.5t/ hour |
Begin to culture after the juvenile fish 3 days, 8 tail death are arranged,, found that the fine worm of shield, be confirmed to be because the fine insect infection disease of shield and death with the autopsy of these fishes.Owing in this pond, do not use insecticides such as formalin, so a large amount of juvenile fish death all arranged every day, culture beginning all death of the flatfish juvenile fish in the pond after 39 days.
Like this, in case the fine insect infection disease of shield occurs, the whole death of the juvenile fish in this pond only are the problems of time so.
[embodiment 1]
As embodiments of the invention 1, in the flatfish plant that the fine insect infection disease of shield occurs, in culturing water, add stabilizing chlorine dioxide, citric acid and hydrogen peroxide and carry out dipping.The variation of the juvenile fish death toll of this situation is shown in Fig. 2.Owing to be the embodiment in other pond in the identical plant with comparative example, so the kind of juvenile fish and cultivating condition are all identical with comparative example.
Among the embodiment 1, breed begins to find the same day 12 tail death.Autopsy be found that the fine worm of shield, be confirmed to be death owing to the fine insect infection disease of shield.
From culturing in 12 days that begin the same day, make juvenile fish in culturing water, having added stabilizing chlorine dioxide, citric acid, hydrogen peroxide and the concentration rate of each medicament is reached in the soup of 1ppm, 0.15ppm, 15ppm, carry out the dipping of 3 hours every days.At this moment, the pond conduct is the darkroom completely, becomes the state that ultraviolet ray is covered fully.In addition, rate of water exchange is with usual the same.
Then, from the 13rd to the 16th 4 days, stopping to change water, make juvenile fish in the soup of above-mentioned concentration, carry out dipping continuously.
Then,, change water, make juvenile fish in the soup of above-mentioned concentration, carry out the dipping of 3 hours every days as at ordinary times rate of water exchange from the 17th to the 28th 12 days.
As shown in Figure 2, during the 17th to the 28th, dead juvenile fish number sharply reduces, culturing beginning the 30th day, the juvenile fish death toll that is caused by the fine insect infection disease of shield is zero, in the past juvenile fish can only all dead pond, can treat shield fibre insect infection disease fully.
In addition, afterwards the dipping of 4 hours every days is proceeded 7, confirm the death that in the pond, does not cause because of the fine insect infection disease of shield.
[embodiment 2]
As embodiments of the invention 2, in the flatfish plant that the fine insect infection disease of shield is taken place similarly to Example 1, in culturing water, add stabilizing chlorine dioxide, citric acid and hydrogen peroxide and carry out dipping.The variation of the juvenile fish death toll of this situation is shown in Fig. 3.Since be with comparative example and embodiment 1 identical plant in other pond in embodiment, so the kind of juvenile fish and cultivating condition are all identical with comparative example and embodiment 1.
Among the embodiment 2, begin to find the same day 9 tail death in breed.Autopsy be found that the fine worm of shield, be confirmed to be death owing to the fine insect infection disease of shield.
Similarly to Example 1, from culturing in 12 days that begin the same day, make juvenile fish in culturing water, having added stabilizing chlorine dioxide, citric acid, hydrogen peroxide and the concentration rate of each medicament is reached in the soup of 1ppm, 0.15ppm, 15ppm, carry out the dipping of 3 hours every days.At this moment, the pond conduct is the darkroom completely, becomes the state that ultraviolet ray is covered fully.In addition, rate of water exchange is with usual the same.
Then, from the 13rd to the 16th 4 days, stopping to change water, make juvenile fish in the soup of above-mentioned concentration, carry out dipping continuously.
Then,, change water, make juvenile fish in the soup of above-mentioned concentration, carry out the dipping of 3 hours every days as at ordinary times rate of water exchange from the 17th to the 28th 12 days.
As shown in Figure 3, after the 19th day, dead juvenile fish number sharply reduces, and from beginning to culture 5 tails is arranged on the 27th among the embodiment 2.Afterwards, the juvenile fish death toll that caused because of the fine insect infection disease of shield on 30th is zero, in the past juvenile fish can only all dead pond, can treat shield fibre insect infection disease fully.
In addition, afterwards the dipping of 4 hours every days is proceeded 7, confirm the death that in the pond, does not cause because of the fine insect infection disease of shield.
[embodiment 3]
As embodiments of the invention 3, for the flatfish plant that the fine insect infection disease of shield is taken place, used as the formalin of bactericide and chlorophos but loseed the pond of result of treatment, in culturing water, add stabilizing chlorine dioxide, citric acid and hydrogen peroxide and carry out dipping.In this plant, in 10 pond, culture following juvenile fish 49900 tails of the long 15cm of body under the conditions shown in Table 6.
Table 6
| Pool size | 7.5mφ |
| Water level | 50cm |
| Water temperature | 21.5～15.5℃ |
| The water yield | 9.8t |
| Rate of |
1～1.5t/ hour |
The variation of juvenile fish death toll in this plant is shown in Fig. 4.Among the embodiment 3, the death of flatfish juvenile fish, confirm that the cause of the death is after the fine insect infection disease of shield, different with embodiment 1 and embodiment 2, at first make juvenile fish will allocate to the soup of 250ppm the dipping that (adding formalin in culturing water) carry out at 1 hour every day (on September 12～14 3 days) as the formalin concentration of bactericide.But the death toll of juvenile fish still increases during this period.
Then make juvenile fish the concentration of stabilizing chlorine dioxide allocated the dipping of carrying out at 4 hours every day to the soup of 10ppm (on September 15～18 4 days) again.But the death toll of juvenile fish does not still reduce during this period.
Afterwards, stopped dipping during 19～22 September, death toll is anxious to be increased, and reaches hundreds of tails every day.Therefore make juvenile fish allocate the dipping of carrying out at 1 hour every day to the soup of 250ppm (September 22 days～24 3 days) once more.Though result's death toll temporarily reduces, increase to nearly thousand tails of death toll every day very soon.
Therefore, make juvenile fish formalin concentration allocated the dipping of carrying out at 1 hour every day to the soup of 250ppm (on September 28～30 3 days), make juvenile fish this formalin concentration allocated the dipping of carrying out at 0.5 hour every day to the soup of 350ppm (October 1～3 3 days) again.As a result, death toll every day October 3 is reduced to below 400 tails.In addition, in October 2 and October 3, select bad individuality and dispose.
Because long-time continuous is in the dipping of the such high concentration of formalin concentration 350ppm, might bring harmful effect to the health of juvenile fish, therefore 6 days of 4～9 October, replace the formalin dipping, make juvenile fish in the soup of concentration being allocated to stabilizing chlorine dioxide 20ppm and chlorophos 0.5ppm (in culturing water, adding stabilizing chlorine dioxide and chlorophos), carry out the dipping of 4 hours every days.October 5, select bad individuality and dispose (3000 tails are put in coexistence in 3 times of October 2, October 3, October 5).But this death toll during 6 days is than increasing to some extent October 3.
Make juvenile fish formalin concentration allocated the dipping of carrying out at 2 hours every day to the soup of 300ppm (on October 9～11 3 days) again.But death toll becomes about 500 tails, does not have the trend that reduces.
Then, consider the health of juvenile fish,, make juvenile fish in the soup that concentration is adjusted to stabilizing chlorine dioxide 10ppm and chlorophos 0.5ppm, carry out the dipping of 5 hours every days at 3 days of October 11～13.As a result, though October 12 death toll temporarily reduce to some extent, October 13 death toll increase to some extent.
Like this, up to October 13, though used formalin, stabilizing chlorine dioxide and three kinds of medicaments of chlorophos, there is not to treat the fine insect infection disease of shield, can not improve the lasting situation of a large amount of juvenile fish death every day.
After October 13, make juvenile fish carry out dipping having added stabilizing chlorine dioxide, citric acid, hydrogen peroxide and each drug concentration ratio is respectively in the soup (adding medicament to culturing water) of 1ppm, 0.15ppm, 15ppm.The dipping time is as shown in table 7.In addition, the pond conduct is the darkroom completely, becomes the state that ultraviolet ray is covered fully.
Table 7
| Date | The dipping time |
| October 13 | 6 |
| October 14 | 12 |
| October 15 | 6 |
| October 16 | Stop dipping |
| October 17 | 4 |
| On October 18～22 | 6 |
| October 23 | Stop dipping |
| 23～November 3 October | 6 |
After beginning to carry out the dipping of soup of stabilizing chlorine dioxide 1ppm, citric acid 0.15ppm, hydrogen peroxide 15ppm, in the October 14 of next day, death toll is than reducing the day before yesterday more than 100 tails.In addition, though do not carry out dipping in October 16 and two days on the 23rd October, death toll through the time reduce, every day is less than 100 tails after October 22.The death toll of the juvenile fish of being caused by the fine insect infection disease of shield November 3 is zero afterwards.
In addition, afterwards the dipping of 4 hours every days is proceeded 7, confirm the death that does not cause in the pond because of the fine insect infection disease of shield.
As implied above, fine worm disease methods of treatment of shield of the present invention and prevention method are for being that the fine insect infection disease of shield of taking place in plant that can not tackle of insecticide is very effective with formalin or organic phosphor.In addition because the medicament that toxicity is low is used in combination, do not exist yet on the juvenile fish health problem and when edible to the problem on the health.
From above-mentioned explanation, those skilled in the art can be apparent to many improvement of the present invention or other embodiment.Therefore, it only is example that above-mentioned explanation should be interpreted as, and is the enforcement best illustration of the present invention that provides to be illustrated as purpose to the technical staff.Under the prerequisite that does not exceed inventive concept of the present invention, can do substantial change to the detailed content of its structure and/or function.
Fine worm disease methods of treatment of shield of the present invention and prevention method are very useful to the breed field of fish.
Claims (6)
1. the methods of treatment of the fine insect infection disease of the shield of fish, it is characterized in that, make fish more than respectively with 0.5ppm and the concentration below the 10ppm, more than the 0.015ppm and below the 0.3ppm, more than the 5ppm and below the 30ppm contain in the soup of stabilizing chlorine dioxide or chlorite, organic carboxyl acid, hydrogen peroxide, carry out dipping to cover ultraviolet state; Described organic carboxyl acid is for being selected from acetic acid, citric acid, malic acid, succinic acid and tartaric more than a kind or 2 kinds.
2. the methods of treatment of the fine insect infection disease of the shield of fish as claimed in claim 1 is characterized in that described fish is flatfish, turbot or filefish.
3. as the methods of treatment of the fine insect infection disease of the shields of claim 1 or 2 described fishes, it is characterized in that the time of dipping is that every day is more than 4 hours.
4. the prevention method of the fine insect infection disease of the shield of fish, it is characterized in that, make fish more than respectively with 0.5ppm and the concentration below the 10ppm, more than the 0.015ppm and below the 0.3ppm, more than the 5ppm and below the 30ppm contain in the soup of stabilizing chlorine dioxide or chlorite, organic carboxyl acid, hydrogen peroxide, carry out dipping to cover ultraviolet state; Described organic carboxyl acid is for being selected from acetic acid, citric acid, malic acid, succinic acid and tartaric more than a kind or 2 kinds.
5. the prevention method of the fine insect infection disease of the shield of fish as claimed in claim 4 is characterized in that described fish is flatfish, turbot or filefish.
6. as the prevention method of the fine insect infection disease of the shields of claim 4 or 5 described fishes, it is characterized in that the time of dipping is that every day is more than 4 hours.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2006-219612 | 2006-08-11 | ||
| JP2006219612 | 2006-08-11 | ||
| JP2006219612A JP3882939B1 (en) | 2006-08-11 | 2006-08-11 | Method for treating and preventing fish succichiosis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101120659A CN101120659A (en) | 2008-02-13 |
| CN101120659B true CN101120659B (en) | 2011-06-01 |
Family
ID=37852264
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007100038691A Active CN101120659B (en) | 2006-08-11 | 2007-01-10 | Method for treating and preventing scuticociliatosis in fishes |
Country Status (4)
| Country | Link |
|---|---|
| JP (1) | JP3882939B1 (en) |
| KR (1) | KR100768143B1 (en) |
| CN (1) | CN101120659B (en) |
| HK (1) | HK1117993A1 (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2445016B1 (en) * | 2008-10-16 | 2015-03-10 | Nat Univ Corp Ehime Univ | VACCINE FOR THE TREATMENT / PREVENTION OF ESCUTICOCILIATOSIS IN FISH |
| US8992831B2 (en) | 2009-09-25 | 2015-03-31 | E. I. Du Pont De Nemours And Company | Stabilized chlorine dioxide to preserve carbohydrate feedstocks |
| JP5221591B2 (en) | 2010-04-23 | 2013-06-26 | ペルメレック電極株式会社 | How to control ectoparasites that infest cultured fish |
| JP5636296B2 (en) | 2011-01-20 | 2014-12-03 | 公益財団法人微生物化学研究会 | Salt-containing organic waste liquid treatment agent, salt concentration reducing agent, salt-containing organic waste liquid treatment method, and comprehensive fixed carrier |
| KR101353200B1 (en) | 2012-01-02 | 2014-01-17 | 나병환 | Method for exterminating and preventing scuticociliate in fishes |
| CN102928274B (en) * | 2012-10-25 | 2014-09-17 | 中国水产科学研究院黄海水产研究所 | Method for depilating cilium on body surface of seawater infusorians |
| CN104255607A (en) * | 2014-09-25 | 2015-01-07 | 广东省昆虫研究所 | Method for simple and rapid removal of tilapia invading aquaculture pond |
| CN105211136A (en) * | 2015-08-31 | 2016-01-06 | 蚌埠龙达农业专业合作社 | A kind of landscape fishpond water sterilization powder and preparation method thereof |
| KR101793393B1 (en) * | 2016-12-27 | 2017-11-02 | 서울대학교산학협력단 | INHIBITOR COMPRISING DINOFLAGELLATES Alexandrium spp. AGAINST SCUTICOCILIATES |
| CN108552216B (en) * | 2018-01-25 | 2021-05-25 | 湖北荷普药业股份有限公司 | Sporicide and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1762382A (en) * | 2005-09-23 | 2006-04-26 | 中国水产科学研究院黄海水产研究所 | Sea fish paralembus digitiformis pesticide prescription |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH067345B2 (en) * | 1987-06-24 | 1994-01-26 | 株式会社 エイ・ティ・ア−ル自動翻訳電話研究所 | Speech recognition method using vector quantization |
-
2006
- 2006-08-11 JP JP2006219612A patent/JP3882939B1/en active Active
-
2007
- 2007-01-10 CN CN2007100038691A patent/CN101120659B/en active Active
- 2007-01-17 KR KR1020070005215A patent/KR100768143B1/en active IP Right Grant
-
2008
- 2008-07-15 HK HK08107785.6A patent/HK1117993A1/en unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1762382A (en) * | 2005-09-23 | 2006-04-26 | 中国水产科学研究院黄海水产研究所 | Sea fish paralembus digitiformis pesticide prescription |
Non-Patent Citations (2)
| Title |
|---|
| 吴晓慧等.牙鲆盾纤虫病及其防治.水产科学18 5.1999,18(5),42-45. |
| 吴晓慧等.牙鲆盾纤虫病及其防治.水产科学18 5.1999,18(5),42-45. * |
Also Published As
| Publication number | Publication date |
|---|---|
| HK1117993A1 (en) | 2009-01-30 |
| JP2008044862A (en) | 2008-02-28 |
| KR100768143B1 (en) | 2007-10-17 |
| JP3882939B1 (en) | 2007-02-21 |
| CN101120659A (en) | 2008-02-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101120659B (en) | Method for treating and preventing scuticociliatosis in fishes | |
| JP2020048468A (en) | Cultivation method of aquatic animal | |
| US5732653A (en) | Modifications to a process for farming crustacea | |
| Owatari et al. | Amyloodiniosis in farmed Sardinella brasiliensis (Steindachner, 1879): Case report of successful therapeutic control with copper sulfate | |
| JP3480566B2 (en) | Natural bioactive substance effective for fish disease and fish feed containing it | |
| Sampson et al. | Novel aspects of Drosophila suzukii (Diptera: Drosophilidae) biology and an improved method for culturing this invasive species with a modified D. melanogaster diet | |
| WO2015056769A1 (en) | Method for exterminating fish-external parasites using low-concentration hydrogen peroxide solution | |
| KR101049863B1 (en) | Artificial feed of brine brine and method of propagation of brine brine using same | |
| JP2000281568A (en) | Agent for preventing and curing parasite | |
| Yu et al. | Larval, juvenile, and adult predators | |
| JP2575240B2 (en) | How to treat freshwater fish | |
| KR101850697B1 (en) | Composition for preventing or treating scuticociliatosis in fishes, and natural feed having the same | |
| EP3422856B1 (en) | Preparation containing sea water and an added potassium compound | |
| KR100373147B1 (en) | Treatment agent of Scuticocilictida | |
| CN111838026B (en) | Domestication and operation method for preventing and controlling parasitic disease of east starfish leech by utilizing scatophagus argus | |
| CN110934136B (en) | Pond-cleaning disinfectant suitable for crustacean culture pond | |
| CN113080115B (en) | Ecological method for removing dactylogyrus on body surface of colorful immortal fish | |
| KR102615461B1 (en) | Ice manufacturing method for providing disinfectant in water while maintaining a mild condition and administration method using the same. | |
| Muthiah et al. | Pests and predators of oysters | |
| CN107873634A (en) | Support the prevention of rotten onychonosus and the treatment method of tortoise in pond | |
| CN107439429A (en) | A kind of cultural method of fresh-water fishes mixed breeding Taihu Lake crab | |
| Liao et al. | The use of chemicals in aquaculture in Taiwan, Province of China | |
| KR100408549B1 (en) | treatment method of artemia cysts | |
| Loose | Martin Fritsch, Jørgen Olesen, Sten Ole Møller, and | |
| Zachariah et al. | Invertebrates |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1117993 Country of ref document: HK |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: GR Ref document number: 1117993 Country of ref document: HK |