KR101353200B1 - Method for exterminating and preventing scuticociliate in fishes - Google Patents

Abstract

Formalin, which has been conventionally used conventionally for Scutika infection of fish, is completely prohibited to be used for farmed fish in the autonomous islands of Jeju Island and Japan. Therefore, it is necessary to develop a new environmentally friendly treatment composition according to the occurrence of Scutika infection in farms. In the present invention, by adding a composition consisting of citric acid, chlorine dioxide, a surfactant and a nano-inorganic substance to aquaculture water as a non-formalin composition for the prevention and treatment of scuticasis so that the cultured fish suffering from Scutica infection can be treated It was.

Description

Composition and prevention methods for controlling ciliary infectious diseases in aquaculture fish {Method for exterminating and preventing scuticociliate in fishes}

The present invention replaces formalin, which has been used in the past, by using a composition consisting of chlorine dioxide, a surfactant, citric acid and nano-inorganic substances for the control of the scouticociliatosis of aquaculture fish caused by parasitism of Scutica citrus. The present invention relates to a composition for preventing skutikaosis and a method for preventing fish.

Surgical Utica increase is a parasite of Surgical Utica chungyi halibut belongs to the Surgical Tycho Celia peptidase neck (Scuticociliatids) kind of ciliate protozoa a disease that mainly endemic etc. seawater and form in terrestrial form, tanks or marine cage fish, especially flounder It occurs by invading the subcutaneous tissue and causing ulcers.

1 shows a Scotch caterpillar seen by the fluorescent antibody method. The worms have the shape of water droplets, 20-40㎛, with cilia throughout the whole body, with 8 to 12 ciliary rows depending on the major axis, and 1 cilia at the tail end.

Squatch caterpillars are active in water, and there are cell cells at one end of the fungus. Originally, it is not a parasitic life, but a protozoan that resides on the bottom or wall of a tank, and a lot of food is used during seedling production, or a large amount is reproduced during overcrowding to kill fish.

 Most of the fish infected with this insect show desquamation of the epidermis, ulcers exposing the muscle layer, and whitening of the caudal fin. In severe cases, the rotational movement in the tank may emit light, because the parasites invade the brain and destroy the brain cells.

When the body is infected with this insect, the mortality is increased by continuously growing in the seedlings.In this case, avoiding dense food and removing organic substances so that they do not remain at the bottom of the tank. When parasitic to the outside such as fins, 100-150ppm formalin has been used for 1 hour bathing.

Formalin is a typical carcinogen, formaldehyde, dissolved in water at a concentration of 35-38%. It is widely used as a disinfectant and antiseptic. In Korea, it is classified as a poison according to the Water Quality Preservation Act (Article 29), the Toxic Chemicals Control Act (Article 15), and the Waste Management Act (Article 25). Are classified as hazardous substances according to the Air Quality Preservation Act (Article 2).

 Meanwhile, the use of formalin as a parasite for fish in aquaculture has been banned in Japan since 2003, when formalin was detected in aquaculture blowfish, which was eventually banned.In July 2006, Jeju Special Self-Governing Province in Jeju Island was launched in aquatic products. The bylaws stipulate that if it is detected, it is forbidden to take it out. However, formalin, which is banned in Japan, can be used in cities and provinces other than Jeju Island, thereby bringing about trade friction with Japan and raising consumer problems.

In fact, in a 1997 report by the Ministry of Maritime Affairs and Fisheries (<Acute Toxicity Effect of Growing Flounder (Flat Flounder) due to Chemical Treatment>), formalin among formalin, chlorine dioxide, and hydrogen peroxide has a higher probability of lethality at lower concentrations than the other two. "It is more effective to use hydrogen peroxide than formalin because it is toxic," he advised to refrain from using formalin.

Therefore, the use of formalin as a therapeutic drug for Scutika insects is no longer difficult to use in Jeju, as well as domestic farms, it is necessary to develop a pharmaceutical composition to replace it, but no special alternative composition has yet to be developed.

As an antiparasitic method or a method for preventing infection of aquaculture fish parasites, Korean Patent Publication No. 10-08235580 discloses a parasitic insecticide containing sodium peroxide (NaClO3) and a quaternary ammonium compound and a parasitic insecticide as a main component. Disclosed are a structure relating to an insecticide insecticide and a method of insecticide of fish including bathing. Accordingly, it is disclosed that minimizing the stress of fish without using formalin, and maximizing the insecticidal efficiency by applying in the form of a mixture rather than the pesticidal efficiency of the component alone. National Registration No. 10-03731470 describes Sukuchika, which uses ALA, an environmentally friendly substance, as a new use of -aminolevulinic acid (ALA), to prevent and treat infectious diseases of the fish parasite Scuticociliatida. The composition of a therapeutic agent for worm infection is disclosed, and domestic application No. 10-2002-0068189 relates to an inactivated vaccine against Scutika, a parasite of fish, which is parasitic in fish. The inoculation of fish to increase the immune ability of the body against the Scutika insects is disclosed to have the effect of preventing the infection and delay the mortality, Korean Patent Publication No. 07350380 and recombinant protein vaccine against Scutika insects and As a composition for the prevention and treatment of squaticosis using the same, a 38kDa recombinant antigenic protein of the squatosis cilia protein was prepared and This was expressed in E. coli vaccine is disclosed. In addition, Korean Patent Publication No. 10-0768143 discloses a stabilized chlorine dioxide or chlorite of 0.5 ppm or more and 10 ppm or less, an organic carbonic acid of 0.015 ppm or more and 0.3 ppm or less, and hydrogen peroxide of 5 ppm or more and 30 ppm or less, respectively, in a water-blocked bath. In addition, a composition for treating fish with Scutika infection by bathing the fish is disclosed. However, the prior art is different from the composition of the composition consisting of chlorine dioxide and a surfactant, citric acid and nano-inorganic material as a non-formalin-based composition for preventing and treating squaticosis as in the present invention, There is also a difference in the remedy effect of reducing the number of dead fish in fish caught.

There is no effective treatment other than formalin baths for Skutika infectious diseases, and formalin, which has been conventionally used conventionally, has been banned for farmed fish in Japan and Jeju Island. There is a need for the development of a new environmentally friendly Succina rescue composition.

It is an object of the present invention to provide a method for preventing and preventing Scutica infection of cultured fish as a means to replace formalin, which has been effectively used in the past.

Accordingly, in the present invention, a non-formalin-based composition for the prevention and treatment of sputicasis is treated by adding a composition consisting of a surfactant, a nano-inorganic substance, citric acid, and chlorine dioxide to aquaculture water to treat aquaculture fish suffering from Skutika infection. To make it possible.

Specifically, the present invention is administered by mixing 30-40 g% by weight of chlorine dioxide, 2-3 g% by weight of citric acid, 3.5-4.5 g% by weight of nano-inorganic minerals, and 2-3 g% by weight of surfactant in cultured water on a one-ton basis. Since the surfactant is not soluble in seawater, it is administered in combination with ethanol or hydrogen peroxide.

The use of formalin, which has been conventionally used as a medicament for Scutika insects, has become difficult to use in aquaculture farms in Korea as well as in Jeju Island. The composition of chlorine dioxide, surfactants, citric acid and nano-inorganic substances composed of non-formalin-based compositions to replace them In addition, it can be treated in an environmentally friendly way to aquaculture fish with Scutika infection by bathing.

1 shows a Scotch caterpillar seen by the fluorescent antibody method.
Figure 2 shows an internal panoramic view of the flounder farm.
Figure 3 shows a photograph of the flounder infected with Squaw.
Fig. 4 shows a photograph of the olive flounder that died due to Scoccosis infection.
Figure 5 shows the daily mortality when using the composition according to the present invention.

In order to achieve the object of the present invention, provided Skutika infectious disease treatment composition is 30-40g% by weight of chlorine dioxide, 2-3g% by weight citric acid 3.5-4.5g% by weight based on 1 ton of cultured water and surfactant It is characterized by mixing 2-3g% by weight.

More specifically, the composition of the inorganic material contained in the 3.5-4.5g% by weight of the nano-inorganic is appropriate to include the ratio of calcium, zinc, magnesium in the ratio of 45: 19: 6, since the surfactant does not mix well in seawater, ethanol or Hydrogen peroxide should be mixed with the surfactant in a proportion. The mixing ratio of surfactant and ethanol is 90-94% by weight: 7-9% by weight is appropriate, and the optimal ratio is 92% by weight: 8% by weight. In the case of mixing surfactant and hydrogen peroxide, the ratio may be mixed in a ratio of 2.5-3.5: 0.5-1.5, and the most suitable ratio is 3: 1.

The mixed composition is added to aquaculture water, and the fish suffering from Scutika infection is bathed for 1 to 2 hours.

In order to achieve the object of the present invention, the overall configuration described above with reference to the embodiment as follows.

1) Composition of the composition

A) Prepare chlorine dioxide (CiO ₂ ) at a rate of 30-40 g% by weight per tonne of aquaculture.

Chlorine dioxide is used as a strong disinfectant deodorant with oxygen, and it dissolves well without any residue in water. The acidity (pH) is 6-9, which has a fast sterilization and deodorization effect, and has a 2.5 times sterilization effect than chlorine, and does not form harmful substances and has low residual toxicity. Chlorine dioxide has been used as a bleach in the past, but it has not been widely used because it is not easy to prepare in a safe and gaseous state. Recently, however, it has been used for various purposes such as removing toxic minerals, removing heavy metals, disinfecting and disinfecting, bleaching clothes, and removing odors.

In particular, it oxidizes ammonia, hydrogen sulfide, mercaptan, phenol, etc., which cause odors, or destroys structures to effectively remove odors. In addition, when sterilized with a chlorine compound, it reacts with organic substances to produce carcinogens such as trihalomethane, but chlorine dioxide does not produce such carcinogens at all.

Therefore, excessive use of chlorine dioxide in aquaculture fish causes fish to be stressed in the bathing process due to strong sterilization action, causing writhing in the water tank.

B) Prepare citric acid at a ratio of 2-3g% by weight per tonne of farmed water.

Citric acid is a chemical formula C ₆ H ₈ O ₇ , soluble in water and ethanol. Citrus, lemons, and less ripe citrus fruits, such as citrus fruits, and especially in water, crystals of water gives a large rod-shaped crystals with one molecule of crystal water.

Citric acid is a component of the TCA cycle and citric acid plays an important role in metabolism of higher animals. It is also known to promote calcium absorption in the body and is used as a blood coagulation inhibitor because it traps calcium ions.

Citric acid as a composition used in the present invention serves to facilitate the absorption of calcium contained in the nano-mineral administered together through the body skin infected with Scutica. In addition, it reacts with citric acid and nano-inorganic material and chlorine dioxide having a strong bactericidal effect to reduce the stress of the fish from chlorine dioxide, and after 48 hours after the reaction with citric acid, the residual chlorine dioxide is not detected in the fish body.

C) Mix nano-minerals at 3.5-4.5g weight per tonne of farmed water.

The composition of the inorganic material contained in the 3.5-4.5g% by weight of the nano-mineral is appropriate that the mixing ratio of calcium, zinc, magnesium in a ratio of 45: 19: 6. Here, nano inorganic means that the particle size of the inorganic material which becomes a raw material of a composition consists of nano size. Nano-inorganic minerals dissolved in seawater, as mentioned above, react with citric acid to absorb minerals in fish organisms, increasing the immunity of fish and preventing harmful bacteria inhabiting the fish.

In addition, according to an embodiment of the present invention, when the nano-mineral is excessively administered, the fish swims in the tank, causing a phenomenon that the feed is not fed for about 5-6 days. Compared with the amount administered, the rescue effect of the squeezes carcinoma is reduced by about 30%, so the proper dose of the nano-inorganic substance is very important in the scavenging effect.

D) Surfactant should be mixed 2-3g weight per tonne of aquaculture water.

Polyoxyethylene sorbitan monooleate was used as a surfactant. Surfactants are substances that dissolve in liquids and significantly reduce the surface tension. They are divided into hydrophilic and hydrophobic parts in the molecule, which makes them easy to adsorb on the interface and is a molecule called micelle above a certain concentration (critical micelle concentration). To form an aggregate. Since sorbitan esters (span), which are commonly used surfactants, are mainly used as emulsifiers, they are almost insoluble in water, so it is appropriate to use nonionic surfactants of tween products made by adding ethylene oxide. Other compositions, which are administered together at the interface, adhere well to the surface of the fish to facilitate infection treatment.

However, these nonionic surfactants are also insoluble in seawater, so ethanol or hydrogen peroxide should be mixed with the surfactant in a certain ratio.

The mixing ratio of surfactant and ethanol is 90-94% by weight: 7-9% by weight is appropriate, the optimal ratio is 92% by weight: 8% by weight. In addition, when the surfactant and hydrogen peroxide are mixed, it can be mixed in a ratio of 2.5-3.5: 0.5-1.5, and the most suitable ratio is 3: 1. In addition, the use of surfactants, citric acid and nano-minerals in combination with chlorine dioxide reacts directly to the schizocara larvae by interfacial attachment of chlorine-dioxide and nano-inorganic constituents to the surface of the fish body infected with succinocosis. This can be used to control Scoccica ciliates more effectively than using chlorine dioxide alone.

2) Example

It looks at the results of the experiment in the culture site using the schichocaemia treatment composition disclosed in the present invention. Figure 2 shows a picture of the inside of the flounder farm, Figure 3 shows a picture of the flounder infected with scoccosis, Figure 4 shows a picture of the halibut died from the infection.

A) Example of Jeju H flatfish farm

The experiment was carried out in H flatfish farm in Jeju Island from January 1, 2010 to 10.8 the same year. Aquaculture water was kept at 20 ~ 21 ℃ and dissolved oxygen was kept at 5ppm. The fish size of the flounder cultivated was less than 21cm and about 4000 rice were grown. The tank size was 10m x 10m, and the water level was about 10cm, and the quantity was about 10t. The water exchange rate was maintained at 1 hour 30 minutes.

At the farm, the disease caused the disease, and the mortality rate was 200, a day. The 10 tons of aquaculture water 350g chlorine dioxide, 30g citric acid, 40g nano-minerals, 25g surfactant and 75ml of hydrogen peroxide mixed well in the cultured water. After mixing the composition in aquaculture water, the bath time was maintained for about 1 hour 30 minutes. Bathing was done in the form of taking a morning bathing and feeding at the same time as usual during the evening feeding, the bathing experiment was carried out for 7 days.

Before starting the bathing, we had 200 tails a day, but after starting the bathing, 160 days on the 1st day, 100 rice on the 2nd day, 60 beauty on the 3rd day, 20 beauty on the 4th day, 10 beauty on the 5th day, 10 beauty on the 6th day There was no death on day 7.

B) Example of Jeju flatfish farm

The experiment was carried out at S flatfish farm in Jeju Island from July 7, 2011 to July 21, the same year. Breeding water temperature was maintained at 22 ~ 23 ℃, dissolved oxygen was maintained at 5ppm. The body size of the fish was less than 19cm and about 5000 fish were grown. The tank size was 12m x 12m in width and length. The water level was maintained at 7 cm and the water quantity was maintained at 10 tons. The return rate of the water tank was set at 1 hour 30 minutes.

The experiment was conducted for 7 days, and mortality was increased by 100 rice per day after the onset. Thus, 10 tons of aquaculture water 350g chlorine dioxide, 30g citric acid, 40g nano inorganic and 25g surfactant and 75ml of hydrogen peroxide mixed well in aquaculture water. The bath time was maintained for about 1 hour 30 minutes with the composition mixed in the cultured water. Bathing was a form of taking a bath in the morning, and at the time of the evening feeding normal feeding as usual.

As a result of the bathing, the mortality was reduced to 70 days on day 1, 50 days on day 2, 10 on day 3, 5 on day 4, 2 on day 5, 2 on day 6, 2 on day 6, and 7 on day 7. Figure 5 shows the daily mortality when using the composition according to the present invention.

The important point in the experiment is that chlorine dioxide is decomposed by ultraviolet rays, so the visible light should be blocked, and in the case of flowing water, the water should not flow in the water tank and watch for 1 hour and 30 minutes while seeing oxygen deficiency in the cultured water. It is appropriate to do so. In addition, if the Skuchika ciliary insects are infected in the brain or in the body, it is judged that there is almost no remedy effect, so it is necessary to quarantine and discard them out of the tank as soon as it is determined that the diseased fish are infected.

As described above, in the present invention, a non-formalin-based composition for the prevention and treatment of sputicasis is treated by adding a composition consisting of chlorine dioxide, a surfactant, citric acid, and a nano-inorganic substance to aquaculture water to treat aquaculture fish suffering from Scutica infection. Could.

A succinicosis control composition of fish consisting of chlorine dioxide and surfactants, citric acid and nano-inorganic substances is a useful remedy to replace formalin, which has been commonly used as a parasite fungicide in fish farming but is increasingly prohibited. It can be utilized as.

Claims (6)

30-40g% by weight of chlorine dioxide, 2-3g% by weight of citric acid, 2-3g% by weight of surfactant and calcium, zinc, magnesium mixture of 45 to 19: 6 3.5-4.5g by weight of aquaculture water Scutika infectious disease control composition of fish, characterized by consisting of%
The method of claim 1, wherein the surfactant is mixed in a certain ratio with any one of ethanol or hydrogen peroxide, the mixing ratio of the surfactant and ethanol is 91-93% by weight: 7-9% by weight, the surfactant And the ratio of hydrogen peroxide is 2.5-3.5: 0.5-1.5 ratio of fish steukica infection control composition, characterized in that the mixture
(A) preparing a nano-organic 3.5-4.5g% by weight consisting of 30-40g% by weight of chlorine dioxide, 2-3g% by weight of citric acid and calcium, zinc, magnesium mixture ratio of 45 to 19: 6 based on 1 ton of aquaculture water ;
(B) mixing 2-3 g% by weight of the surfactant with any one solvent selected from ethanol or hydrogen peroxide;
(C) mixing the surfactant mixed with the solvent of step (B) with the composition of step (A), and adding the mixed composition to the cultured water to bathe the fish for 1 to 2 hours. How to get rid of fish's Scutika infection
The method according to claim 3, wherein the mixing of the solvent of any one selected from ethanol or hydrogen peroxide of step (B) is 91-93% by weight: 7-9% by weight, when the surfactant and ethanol are mixed. In the case of mixing hydrogen peroxide, the method of controlling fish Scutika infectious diseases, characterized in that the mixing ratio of 2.5-3.5: 0.5-1.5 delete delete

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