JP5988240B2 - Ciliate control agent, ciliate control method and water purification agent - Google Patents

Description

The present invention, ciliates pesticides combating ciliates such Sukuchika parasitic on fish, disinfect disinfecting and water ciliates of fiber caterpillars such on water quality purifying agent for purifying water.

  Ciliates live a wide range in nature. Some of them are parasitic and cause damage to aquatic products. In particular, sukutika (Miamiensis avidus) has become a major problem in the field of fisheries because it infests flounder, Thailand and puffer fish and kills infected fish. The percentage of scotchosis in flounder pests varies widely from year to year. However, once it occurs, it is likely to occur repeatedly and the mortality rate of infected fish is high, which may cause catastrophic damage to aquaculture farmers.

  Many methods have been proposed to solve the above problems. For example, there is a method for preventing fish from scourtica infection in aquaculture farms by bathing fish in a chemical solution containing stabilized chlorine dioxide, acetic acid, and hydrogen peroxide at a predetermined concentration while blocking ultraviolet rays (for example, Patent Documents) 1). In addition, as an anthelmintic composition having a therapeutic effect for parasitic diseases of cultured fish using a plant extract, one selected from the group consisting of nib (Azadirachta indica), timo (Thymus vulgaris) and sera (Cymbopogon citratus) There exists a composition for fish parasite anthelmintics containing the above plant extract as an active ingredient (for example, refer patent document 2). Further, as an orally administrable parasite-preventing composition that is less affected by drug side effects and drug residue on fish, there is a composition containing an extract of kudin as a crude drug (see, for example, Patent Document 3).

Japanese Patent No. 3882939 JP 2007-131611 A Japanese Patent No. 4608255

  In the future, parasite damage such as ciliates is expected to increase in the fishery field, and the development of highly safe drugs is eagerly desired. Drugs containing plant-derived components that have a small effect on humans and other organisms as active ingredients are preferred drugs in terms of safety, and among these drugs, drugs that have an excellent effect on the control of ciliates Can be suitably used as a ciliate insecticide. Moreover, in the case of the chemical | medical agent which uses a plant-derived component as an active ingredient, it is important in the point of practical use that this plant is easy to acquire or that cultivation is possible.

An object of the present invention, human and other plant-derived components influence is small with respect to organisms as an active ingredient, ciliates pesticides showing an excellent effect for combating ciliates, Disinfecting and quality of fiber caterpillar acids It is to provide a cleaning agent.

  As a result of screening mainly on native plants, vegetables, and flower buds that can be collected and cultivated in Japan and that have little adverse effect on the ecosystem, we have discovered several types of plants that have the effect of suppressing the growth of sukutika. Among the broccoli that showed the strongest growth inhibitory effect, the active ingredient was isolated and identified, and it was found that sulforaphane was a substance showing a sukutika growth inhibitory effect, thereby completing the invention. The present invention is as follows.

  The present invention is a ciliate insecticide containing sulforaphane as an active ingredient.

In the ciliate insecticide of the present invention, the sulforaphane can be given in one or more forms selected from the following group (A).
(A) Chemically synthesized products, sulforaphane isolated and purified from plants, extracts of plants containing sulforaphane, plants containing sulforaphane, crushed and / or pulverized plants containing sulforaphane, and glycosides of sulforaphane A plant extract, a plant containing a sulforaphane glycoside, and a crushed and / or pulverized product of a plant containing a sulforaphane glycoside.

  Moreover, this invention is a ciliate extermination method which adds the said ciliate insecticide to fresh water, seawater, brackish water, or aqueous solution, and removes ciliates in fresh water, seawater, brackish water, or aqueous solution.

  Further, the present invention, the ciliate insecticide is introduced into a water tank, ginger or container in which fresh water, seawater, brackish water or an aqueous solution is stored or filled, and ciliate or water tank in fresh water, seawater, brackish water, an aqueous solution, It is a ciliate extermination method for exterminating ciliates that adhere to ginger or the wall surface or water tank of a ginger, stone, sand, plants, etc. in the ginger or vessel.

  The present invention also includes a filtration device that filters and removes solids containing suspended solids in freshwater, seawater, brackish water, or aqueous solutions, while circulating freshwater, seawater, brackish water, or aqueous solutions between aquariums, ginger, or containers. It is equipped with a circulating filtration tank to be used, or a filtration device that filters and removes solids containing suspended solids in freshwater, seawater, brackish water, or aqueous solution, and the filtered freshwater, seawater, brackish water, or aqueous solution is put into the aquarium, ginger, or container. The ciliate insecticide is introduced into a flow-through type filtration tank to be sent, and ciliates in fresh water, seawater, brackish water or an aqueous solution, ciliates attached to the solid matter containing the suspended solids, the filtration tank A ciliate extermination method for exterminating ciliates adhering to a wall surface or the filtration device.

  Further, the present invention provides the ciliate insecticide to fresh water, seawater, brackish water or a beach inhabited by aquatic products such as fish, crustaceans, shellfish, and ciliates and freshwater, seawater parasitic on the aquatic products. It is a method for controlling ciliates that control ciliates inhabiting brackish water or beaches.

  The present invention also provides a ciliate for controlling ciliates parasitizing aquatic products by introducing aquatic products such as fish, crustaceans and shellfish into fresh water, seawater, brackish water or aqueous solution to which the ciliate insecticide is added. It is a kind of extermination method.

The present invention is fed a diet containing sulforaphane as an active ingredient of combating ciliates, fish, crustaceans and ciliates and / or the marine organisms parasitic on aquatic organisms such as shellfish inhabiting It is a method for controlling ciliates that control ciliates in the environment.

  The present invention also includes freshwater, seawater, brackish water, ciliates in an aqueous solution, or freshwater, seawater, brackish water, a water tank in which an aqueous solution is stored or filled, a wall surface or water tank of a container, stones in the container, sand, plants, etc. It is a water purification agent containing sulforaphane as an active ingredient that removes ciliates adhering to water and purifies water quality.

Ciliate pesticide of the present invention is a drug agent to a human or other active ingredients influence is small sulforaphane against organisms show an excellent effect for combating ciliates. Moreover, ciliates can be effectively controlled by using the ciliate control method of the present invention. The water purification agent of the present invention is a water purification agent comprising sulforaphane, which has a small effect on humans and other living organisms, and effectively eliminates ciliates by using the water purification agent. Water quality can be purified. Sulforaphane, because it contains a lot broccoli, obtain an easy, ciliates pesticides及 beauty water purification agents of the present invention is easy to put to practical use.

It is a figure which shows the scoutica extermination experiment result using sulforaphane. It is a microscope picture at the time of the extermination experiment of the weevil using sulforaphane. It is a microscope picture at the time of the extermination experiment of the weevil using sulforaphane. It is a microscope picture at the time of the extermination experiment of the weevil using sulforaphane. It is a microscope picture at the time of the extermination experiment of the weevil using sulforaphane. It is a microscope picture at the time of the extermination experiment of the weevil using sulforaphane. It is a microscope picture at the time of the extermination experiment of the weevil using sulforaphane. It is a microscope picture at the time of the extermination experiment of the weevil using sulforaphane. It is a microscope picture at the time of the extermination experiment of the weevil using sulforaphane. It is a microscope picture at the time of the extermination experiment of the weevil using sulforaphane.

  The ciliate insecticide of the present invention is a ciliate insecticide containing sulforaphane as an active ingredient. The ciliate insecticide of the present invention can be used to control a wide range of ciliates and suppress growth, and can be particularly suitably used to control and suppress growth of schootkas and worms.

  Sulforaphane is a kind of isothiocyanate and is widely contained in cruciferous plants such as broccoli, cauliflower, cabbage, and kale, and particularly in broccoli. The content of sulforaphane in broccoli is 44.3 to 171.3 mg / 100 g-dry plant for mature plants and 1153.0 mg / 100 g-dry plant for sprout (Source: Nakagawa, et al. J. Agric. Food Chem. 2006; 54: 2479-2483). It exists mainly in the form of sulforaphane glycosides in plants, and sulforaphane glycosides are hydrolyzed by myrosinase to form sulforaphane.

  About sulforaphane, effects on human diseases such as drugs for the treatment of glaucomatous retinopathy and optic neuropathy (for example, Patent Publication No. 2011-46736) and colon cancer preventive agents (for example, Patent Publication No. 2005-60229), Methods for detoxifying harmful compounds (for example, Japanese Patent Publication No. 2009-154029), bactericidal effect of Helicobacter pylori (Source: Fahey, et al., Proc. Natl. Acad. Sci. USA. 2002; 99 (11): 7610- 7615), but no effect on ciliates. It works as a useful functional ingredient for humans and is considered to have little adverse effect.

  Various forms of sulforaphane can be used as the sulforaphane used in the ciliate insecticide. Sulforaphane isolated and purified from plants such as broccoli, or chemically synthesized sulforaphane can be used. Other than this, an extract of a plant containing sulforaphane, a plant containing sulforaphane, a crushed and / or pulverized product of a plant containing sulforaphane, an extract of a plant containing a glycoside of sulforaphane, a plant containing a glycoside of sulforaphane, Plant crushed material and / or pulverized material containing glycoside of sulforaphane can be used. These may be used alone or in combination of two or more of a plant extract containing sulforaphane and a crushed and / or pulverized product of the plant containing sulforaphane.

  A plant extract containing sulforaphane and a plant extract containing sulforaphane glycoside can be obtained by extracting a plant containing sulforaphane and a plant containing sulforaphane glycoside using a solvent. The extraction method is not limited to a specific method. For example, water and ethanol may be used as an extraction solvent, and broccoli or the like may be immersed in the extraction solvent at room temperature for 1 to 24 hours. As the extraction solvent, a solvent having a small influence on humans and other living organisms is used.

  An acidic solvent may be used as the extraction solvent, but it is not preferable to use an alkaline solvent as the extraction solvent or to make the extract alkaline, since the ability to control ciliates is reduced. The extract of broccoli plant was adjusted to pH 3 with acid (hydrochloric acid) or pH 11 with alkali (ammonia water), and scooter extermination experiments using this were conducted. In the case of pH 3 treatment, a growth inhibition rate of 100% was obtained. As shown, in the case of pH 11 treatment, the growth inhibition rate was 0 and showed no growth inhibition ability.

  Plants containing sulforaphane, crushed and / or pulverized plants containing sulforaphane, plants containing glucosides of sulforaphane, plant crushed and / or pulverized products containing sulforaphane glycosides, Therefore, plants containing sulforaphane can be used instead of sulforaphane.

  The ciliate insecticide can be used as a liquid, granule, or tablet by a known method. The method of using the ciliate insecticide is not limited to a specific method. For example, by adding a ciliate insecticide to fresh water, seawater, brackish water or an aqueous solution, these ciliates in the water can be controlled. In addition, ciliate insecticides in freshwater, seawater, brackish water and aqueous solutions can be introduced into tanks, ginger or containers in which freshwater, seawater, brackish water or aqueous solutions are stored or filled. Ciliates that adhere to the wall or water tank of the container, ginger or stones, sand, plants, etc. in the container can be controlled.

  Similarly, it is equipped with a filtration device that filters and removes solids containing suspended solids in freshwater, seawater, brackish water, or aqueous solutions, and is used while circulating freshwater, seawater, brackish water, or aqueous solutions between aquariums, ginger, or containers. A circulating filtration tank or a filtration device that filters and removes solids containing suspended solids in freshwater, seawater, brackish water, or aqueous solution, and feeds filtered freshwater, seawater, brackish water, or aqueous solution to aquarium, ginger, or container Ciliate insecticide in fresh water, seawater, brackish water or aqueous solution, ciliate attached to solids containing suspended solids, wall of filtration tank or filtration device by introducing ciliate insecticide into sink type filtration tank Ciliate worms attached to can be controlled.

  The method described above is a method for controlling ciliates that adhere to water or the wall surface of a water tank, stones, etc., but ciliates that are parasitic on aquatic products can also be controlled. In this case, ciliate insecticide is administered to freshwater, seawater, brackish water or beach where fishery products such as fish, crustaceans and shellfish live, and ciliate and freshwater, seawater, seawater, What is necessary is just to get rid of ciliates inhabiting brackish water or beaches. In addition, using ciliate insecticide as a bath salt, injecting aquatic products such as fish, crustaceans and shellfish into fresh water, seawater, brackish water or aqueous solution to which ciliate insecticide is added, and parasitic on aquatic products It can also control ciliates.

  A feed containing sulforaphane as an active ingredient for controlling ciliates is also included in the present invention. As the sulforaphane used in the feed of the present invention, a sulforaphane isolated and purified from a plant such as broccoli, or a chemically synthesized sulforaphane can be used in the same manner as the ciliate control agent. Other than this, an extract of a plant containing sulforaphane, a plant containing sulforaphane, a crushed and / or pulverized product of a plant containing sulforaphane, an extract of a plant containing a glycoside of sulforaphane, a plant containing a glycoside of sulforaphane, Plant crushed material and / or pulverized material containing glycoside of sulforaphane can be used. These may be used alone or in combination of two or more of a plant extract containing sulforaphane and a crushed and / or pulverized product of the plant containing sulforaphane.

  The feed of this invention should just mix | blend the sulforaphane of the said form with the conventional feed given to the aquatic products like fish, shellfish, and shellfish which are object. By feeding the feed of the present invention, sulforaphane is orally administered, and ciliates parasitizing aquatic products such as fish, shellfish and shellfish are controlled. Furthermore, when the feed is dissolved in water or dispersed in water, sulforaphane is eluted in water, and ciliates in the environment such as fresh water, seawater, brackish water, aqueous solution, and beach where the aquatic products inhabit can be controlled.

  Furthermore, the present invention also includes a water purification agent containing sulforaphane as an active ingredient, which removes ciliates in fresh water, seawater, brackish water or aqueous solutions and purifies the water quality. As the sulforaphane used in the water purification agent of the present invention, a sulforaphane isolated and purified from a plant such as broccoli or a chemically synthesized sulforaphane can be used in the same manner as the ciliate control agent. . Other than this, an extract of a plant containing sulforaphane, a plant containing sulforaphane, a crushed and / or pulverized product of a plant containing sulforaphane, an extract of a plant containing a glycoside of sulforaphane, a plant containing a glycoside of sulforaphane, Plant crushed material and / or pulverized material containing glycoside of sulforaphane can be used. These may be used alone or in combination of two or more of a plant extract containing sulforaphane and a crushed and / or pulverized product of the plant containing sulforaphane.

  When a water purification agent containing sulforaphane as an active ingredient is added to water, seawater, brackish water or an aqueous solution, it is dissolved in water or dispersed in water, and sulforaphane is eluted in water. Thereby, ciliates in fresh water, seawater, brackish water or aqueous solution can be removed and the water quality can be purified. Also, by introducing a ciliate insecticide into a tank or container in which fresh water, seawater, brackish water, or an aqueous solution is stored or filled, not only ciliates in fresh water, seawater, brackish water, and aqueous solutions, Ciliates that adhere to stones, sand, and plants in water tanks and containers can be removed to purify water quality.

  As described above, the ciliate control agent and the feed of the present invention are a drug and a feed containing sulforaphane as an active ingredient having a small effect on humans and other living organisms, and have an excellent effect on the control of ciliates. . Moreover, ciliates can be effectively controlled by using the ciliate control method of the present invention. The water purification agent of the present invention is a water purification agent comprising sulforaphane, which has a small effect on humans and other living organisms, and effectively eliminates ciliates by using the water purification agent. Water quality can be purified. Since sulforaphane is abundantly contained in broccoli, it is easy to obtain. The ciliate extermination agent, feed and water purification agent of the present invention, ciliate extermination method using the agent, and water purification method are practical. Easy to convert.

<Construction conditions of test material and extract>
250 mg of a plant body (broccoli seed or sprout) dried at 60 ° C. for 24 hours was extracted with 5 mL of pure water at 20 ° C. for 1 hour, and the centrifuged supernatant was sterilized by filtration with a 0.45 μm membrane filter.

<Bioassay method using sukutika>
A. Method by Counting under a Microscope In the following experiment, the scooter used was distributed from the Tottori Prefectural Cultivation Fisheries Center. Sulforaphane or an extract and 2 mL of a schucchia culture solution adjusted to 30 to 40 individuals / μL were added to a φ30 mm petri dish and mixed, and after culturing at 25 ° C. in the dark for 24 hours, the number of surviving scourts was counted under a microscope. At this time, a petri dish to which sulforaphane or an extract was added was used as a treatment group, and a petri dish without addition was used as a control group.
B. Density measurement of scourtica by absorbance method The 96-well microplate was dispensed with the extract and 200 μL of scourtica culture solution adjusted to 30-40 individuals / μL, and cultured at 25 ° C. in the dark for 4 days. At the start and end of the culture, the absorbance at 595 nm was measured using a microplate reader to calculate the growth inhibition rate of sukutika. The method for calculating the growth inhibition rate is according to equation (1). The higher the growth inhibition rate, the higher the extermination effect. Although this method allows simple and rapid analysis, it is not applicable when the number of scourtica individuals is lower than that at the start of culture (30 to 40 animals / μL).

<Plant type, growth stage and extermination effect>
A scout deer control test using cruciferous plants was conducted as follows. The schiotka culture solution to which the extract was added so as to be 0.5 mg dry plant / mL was dispensed into a 96-well microplate, cultured at 25 ° C. in the dark for 4 days, and the absorbance at 595 nm was measured. At the start and end of the culture, the absorbance at 595 nm was measured using a microplate reader to calculate the growth inhibition rate of sukutika.

  Table 1 shows the names of the plants used, their sites (growth stages), and the measurement results of the growth inhibition rate. In addition to broccoli, it was confirmed that there are several cruciferous plants that have the effect of suppressing the growth of scutchia. Furthermore, even in the same plant, the degree of inhibition varies depending on the time and site. In broccoli, it is known that the content of sulforaphane changes depending on the growing season, and this time, it is considered that the difference in the growth inhibition rate of the same plant is due to the difference in the content of sulforaphane in the plant.

<Disinfection effect in a medium with a NaCl concentration of 3% similar to seawater>
The NaCl concentration of seawater is approximately 3%, which is higher than that of 0.8% of the scourtica medium. Therefore, the control effect on scoutica growing on 3% and 0.8% NaCl media was examined. The NaCl concentration of the normal medium was 0.8%, and a rapid change in the NaCl concentration of the medium inhibited the growth of sukutika, so the test was performed after acclimatization to 3% NaCl medium. Add 0.005, 0.017, 0.05, 0.167, 0.5, 1.667 mg dry plant / mL to scourt culture medium, and after culturing at 25 ° C in the dark for 24 hours, Were counted. The amount of scoutica grown was measured by counting under a microscope.

  The results are shown in Table 2. Broccoli plant extracts in both 3% and 0.8% NaCl media showed similar growth inhibition. For this reason, it is considered that even in seawater with a NaCl concentration of about 3%, the effect of inhibiting the growth of sukutika is equivalent to that in the medium. The reason why the number of scourtica at the end varies depending on the NaCl concentration is that the growth rate in 3% NaCl medium is slow.

<Relationship between treatment concentration and time>
Sulforaphane (purchased reagent) or broccoli dried seed extract rich in sulforaphane is added to the culture solution of sukutika and cultured at 25 ° C. in the dark, and cultured 2, 4, 6, 8, 12, 16, 20 24, 28, 36, and 48 hours, the number of surviving individuals in the culture was counted. The sulforaphane concentrations were 6 types of 0, 0.3, 1.0, 3.0, 10 and 30 μg per 1 mL of the culture solution. The broccoli dry seed extract was made into two types of 0.3 and 1.0 mg dry plant per 1 mL of the culture solution.

  The results are shown in FIG. The exterminating effect on scoutica was obtained at a sulforaphane concentration of 1 μg / mL or higher in the medium. The higher the concentration, the shorter the onset time of the control effect against the scoutica, which was 90% or more after 30 hours at 30 μg / mL, 6 hours at 10 μg / mL, 16 hours at 3 μg / mL. At 1 μg / mL, a weak extermination effect was observed until 28 hours later, but thereafter only a growth inhibitory effect was observed. Similar results were obtained with sulforaphane purified from broccoli seeds.

  As with sulforaphane (purchased reagent), the broccoli dried seed extract also had a shorter control time against scourtica as the concentration was higher. With broccoli dried seeds, the insecticidal rate was 90% or more after 24 hours at a concentration of 0.3 mg dry plant / mL after 12 hours at 1 mg dry plant / mL.

<Experimental experiment of tsuryanemushi using sulforaphane (purchased reagent)>
An artificial sewage and a carrier for holding microorganisms were put in the container and aerated, and the microorganisms containing the beetle were grown. The artificial sewage continued to flow so that the entire amount in the container was replaced in 10 to 12 hours. Microorganisms were collected from the carrier, a suspension of the microorganisms was prepared, and 2 mL each was dispensed into a φ30 mm petri dish, and static culture was performed at 25 ° C. in the dark for 24 hours to fix the beetle on the petri dish surface. Thereafter, dust was removed together with artificial sewage in the petri dish. Artificial sewage 2 mL and sulforaphane were newly added and mixed, followed by static culture at 25 ° C. in the dark for 24 hours.

  The concentration of sulforaphane to be added was 0, 0.3, 1.0, 3.0 and 6.0 μg per 1 mL of the culture solution. The micrograph immediately after adding sulforaphane and mixing, and the micrograph after 25 degreeC, a dark place, 24 hours, and stationary culture were shown in FIGS. Table 3 shows the observation results after standing culture at 25 ° C., in the dark, for 24 hours. 2 to 6, (A) is immediately after adding sulforaphane, and (B) is after static culture at 25 ° C. in the dark for 24 hours.

  As a result of the experiment, the worm was exterminated when the sulforaphane concentration in the culture solution was 3 μg / mL or more. In addition, the form of the beetle at the start of the treatment was normal, and cilia and handle stretching movements were observed.

<Experimental experiment for tsutsugamushi using broccoli seed extract>
After extracting 100 g of broccoli seeds dried at 60 ° C. for 24 hours with 1 L of water for 1 hour, a part was taken and sterilized by filtration. This sterilized extract was used in this experiment.

  An artificial sewage and a carrier for holding microorganisms were put in the container and aerated, and the microorganisms containing the beetle were grown. The artificial sewage continued to flow so that the entire amount in the container was replaced in 10 to 12 hours. Microorganisms were collected from the carrier, a suspension of the microorganisms was prepared, and 2 mL each was dispensed into a φ30 mm petri dish, and static culture was performed at 25 ° C. in the dark for 24 hours to fix the beetle on the petri dish surface. Thereafter, dust was removed together with artificial sewage in the petri dish. Artificial sewage 2 mL and an extract were newly added and mixed, followed by stationary culture at 25 ° C. in the dark for 24 hours.

  The amount of the extract to be added was 0, 1.0, 3.0, and 10 mg dry plant per 1 mL of the culture solution. 7 to 10 show micrographs immediately after the extract was added and mixed, and microphotographs after stationary culture at 25 ° C. in the dark for 24 hours. Table 4 shows the observation results after standing culture at 25 ° C., in the dark, for 24 hours. 7 to 10, (A) is immediately after adding and mixing the extract, and (B) is at 25 ° C., dark place, 24 hours, after static culture.

  As a result of the experiment, when the dry seed concentration in the culture solution was 1 mg dry plant / mL or more, the worm was exterminated, and the purified product from the broccoli seeds had an extermination effect similar to the purchased reagent. In addition, the shape of the worm was normal at the start of the treatment, and cilia and handle stretching movements were observed. In addition, the background of the section to which the extract is added is whitish due to turbidity derived from the extract.

Claims (9)

  A ciliate insecticide containing sulforaphane as an active ingredient. The ciliate insecticide according to claim 1, wherein the sulforaphane is given as one or more forms selected from the following group (A).
(A) Chemically synthesized products, sulforaphane isolated and purified from plants, extracts of plants containing sulforaphane, plants containing sulforaphane, crushed and / or pulverized plants containing sulforaphane, and glycosides of sulforaphane A plant extract, a plant containing a sulforaphane glycoside, and a crushed and / or pulverized product of a plant containing a sulforaphane glycoside.   A method for controlling ciliates, wherein the ciliate insecticide according to claim 1 or 2 is added to fresh water, seawater, brackish water or an aqueous solution, and ciliates in fresh water, seawater, brackish water or an aqueous solution are removed.   The ciliate insecticide according to claim 1 or 2 is introduced into a water tank, ginger or container in which fresh water, seawater, brackish water or an aqueous solution is stored or filled, and the ciliate in fresh water, seawater, brackish water or an aqueous solution, or A ciliate extermination method for exterminating ciliates attached to a water tank, a ginger or a wall surface of a water tank, a ginger, a stone, sand, or a plant in the ginger.   It is equipped with a filtration device that filters and removes solids containing suspended solids in freshwater, seawater, brackish water, or aqueous solutions, and uses a circulation system that circulates freshwater, seawater, brackish water, or aqueous solutions between aquariums, ginger, or containers. It is equipped with a filtration tank or a filtration device that filters and removes solids containing suspended solids in freshwater, seawater, brackish water, or aqueous solution, and is a flow-through type that sends filtered freshwater, seawater, brackish water, or aqueous solution to aquarium, ginger, or container The ciliate insecticide according to claim 1 or 2 is introduced into a filtration tank, and ciliates in fresh water, seawater, brackish water or an aqueous solution, ciliates attached to a solid containing the suspended solids, and the filtration A ciliate extermination method for exterminating ciliates adhering to a wall surface of a tank or the filtration device.   The ciliate insecticide according to claim 1 or 2 is administered to fresh water, seawater, brackish water, or a beach inhabited by aquatic products such as fish, crustaceans, and shellfish, and ciliates and freshwater parasitic on the aquatic products. A method for controlling ciliates that exterminates ciliates inhabiting seawater, brackish water or beaches.   Aquatic products such as fish, crustaceans and shellfish are added to fresh water, seawater, brackish water or aqueous solution to which the ciliate control agent according to claim 1 or 2 is added to control ciliates parasitizing the aquatic products. How to control ciliates. Sulforaphane was fed a diet containing as an active ingredient of combating ciliates, fish, crustaceans, ciliates parasitic in marine organisms, such as shellfish and / or ciliates environment in which the aquatic organisms inhabiting How to control ciliates that control moss.   Ciliates in freshwater, seawater, brackish water, aqueous solutions, or ciliates that attach to freshwater, seawater, brackish water, water tanks or containers filled with aqueous solutions, container walls or tanks, stones, sand, plants in containers, etc. Water purification agent containing sulforaphane as an active ingredient to remove water and purify water.