CN107267582A - The preparation method of sea lettuce active peptides - Google Patents
The preparation method of sea lettuce active peptides Download PDFInfo
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- CN107267582A CN107267582A CN201710482199.XA CN201710482199A CN107267582A CN 107267582 A CN107267582 A CN 107267582A CN 201710482199 A CN201710482199 A CN 201710482199A CN 107267582 A CN107267582 A CN 107267582A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
- C07K1/303—Extraction; Separation; Purification by precipitation by salting out
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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Abstract
The invention discloses the preparation method of sea lettuce active peptides, specifically include:1)Sea lettuce amidin is put into cell crushing instrument inner cell to crush, filtered, by filtrate ultrasonication, centrifugation;2)(the NH of various concentrations is added in centrifugate4)2SO4, static, centrifugation;3)Salt precipitation thing is dissolved in phosphate buffer, in ddH2Dialysed in O, freeze-drying produces sea lettuce albumen;4)Sea lettuce albumen is added water mixing, plus trypsase enzyme and papain enzymolysis, go out enzyme, cooled down, centrifugation;5)Enzymolysis liquid ultrafiltration is produced into sea lettuce active peptides.Have the beneficial effect that:Preparation method technique of the present invention is easy and effective, and production cost is low, and yield is big, is adapted to big rule production;Obtained active peptides have stronger antioxidation activity, can remarkably promote the synthesis of collagen in human fibroblasts, the activity with anti-skin photoage activity.
Description
Technical field
The present invention relates to biological product technical field, more particularly, to the preparation method of sea lettuce active peptides.
Background technology
Ulva Chlorophyta, Chlorophyceae, Ulvales, Ulvaceae, Ulva, its frond are in yellow green, there is two layers of groups of cells
Into membranous body, edge is wide up to cm.In being grown in sea lettuce, on the rock of low tide band or Shi Zhaozhong more, southern species
Color is partially yellow, and northern species color is partially green, and frond contains multivitamin, ergosterol, and acid and third, fourth, penta, 14,
Hexadecylic acid organic acid etc..Sea lettuce is a kind of tangleweed, is distributed widely in Western Pacific coastal.0-35 DEG C of its suitable temperature range, is fitted
Salt scope 15-35%, with very strong vitality and regeneration power, growing pullets are bold and resolute, more prosperous in the fertile inner bay growth of water quality
Contain, be resource extremely abundant one kind in China's wild algae.Not only frond is bright-coloured for it, delicious flavour, and rich in many battalion
Material is supported, with very high economic value.But its value in marine alga processing industry can not show a candle to brown alga and red algae, thus
Usually as the accumulation of natural waste disposal, serious environmental pollution and the wasting of resources is caused.
Drystone water shield shows that main component has crude fibre, carbohydrate, protein and ash content, and contains by chemical analysis
Have in a small amount of fat, sea lettuce also containing various trace elements such as K, Na, Ca, Mg, Ni, Zn, Mo, Cu.Protein content is higher, and one
As content between 17~26%, the sea lettuce protein contents of different sources is not quite similar, the factor such as season also can device to hole stone in addition
Protein content in water shield is produced containing abundant amino acid in certain influence, sea lettuce protein, wherein it is people to have 8 kinds
Body essential amino acid.Sea lettuce function first recorded in《Bencao Shiyi》, it is day " sea lettuce is given birth to the South Sea, attached stone and given birth to, and like seaweed, color is blue or green ", main
" sweet nontoxic, to enter water, diuresis ",《Compendium of Materia Medica》Described in call " going out Fujian, Zhejiang person, great Ye is like dish ", therefore also name Ulva lactuca L has
The effects such as softening and resolving hard mass, clearing heat and detoxicating, sharp water lipid-loweringing.Pharmacological research shows, sea lettuce is in reducing blood lipid, hypoglycemic, antiviral, anti-swollen
The many-sides such as knurl, anti-oxidant and gram raw effect have all shown stronger bioactivity.Sea lettuce can make edible seaweed and eat, and have clear
Mouthful, removing toxic substances, anti-inflammatory the effects such as, can be used for preventing and treating heatstroke, detumescence, soothe sore throat, enterogastritis, except halitosis etc..
Prior art such as Authorization Notice No. is the B of CN 102851345 Chinese invention patent, discloses chlorella antitumor
The preparation method of polypeptide, extracts chlorella albumen, then use trypsase using low-temperature ultrahigh-pressure continuous flow cell disintegrating machine first
Chlorella albumen is hydrolyzed, ultra-filtration centrifuge tube filtering obtains molecular size range scope and is 0-3KD, 3-5KD, 5-10KD and is more than
10KD chlorella polypeptide.Chlorella polypeptide prepared by this method has certain suppression to human liver cancer cell HepG2 growth in vitro
Make and use, be conducive to the exploitation of antineoplastic health product and medical product, but long preparation period, chlorella polypeptide yield
It is low.
The content of the invention
It is an object of the invention to provide a kind of active protein peptide preparation efficiency is high, yield is high, purity is high, energy consumption is low, right
The preparation method of environment-friendly sea lettuce active peptides.
The present invention is directed to the problem of being mentioned in above-mentioned technology, and the technical scheme taken is:The preparation side of sea lettuce active peptides
Method, including clasmatosis, saltout, dialyse, proteolysis, ultrafiltration, specifically include following steps:
Clasmatosis:Ulva lactuca powder is pressed 1:14-16 solid-liquid ratios add water mixing, are put into 4-6 DEG C of temperature, flow velocity 18-21mL/min, pressure
Power 110-130MPa, circulate clasmatosis carried out in the cell crushing instrument of 2-3 time, filtering, by filtrate temperature be 40-50 DEG C,
Power is 200-300W ultrasonication 25-35min, then the refrigerated centrifuge centrifugation through 3-5 DEG C, 7000-9000rpm
18-22min, produces protein extract, and the step combination cell crushing instrument carries out breaking-wall cell with ultrasonic wave, does not influence protein
Activity, can greatly shorten the time of breaking-wall cell, improve working effect, can quantitatively crush frustule, it is adaptable to work
Industry metaplasia is produced;
Saltout:(NH is added into protein extract4)2SO4To 20-25% saturation degrees, static 15-20min continuously adds (NH4)2SO4To 50-55% saturation degrees, then static 15-20min centrifuges 20-25min under 3-5 DEG C, 4000-6000rpm, produces salt
Analyse in sediment, the step and add substantial amounts of ammonium sulfate, protein is obtained solubility reduces and Precipitation, and this method advantage exists
There is continuity in ammonium sulfate concentrations change, effect of saltouing is good, save energy consumption, and using the ammonium sulfate of various concentrations, can
Shorten the sedimentation time, work well;
Dialysis:Salt precipitation thing is dissolved in phosphate buffer, bag filter is then charged into, in ddH2Dialyse, change in O
ddH2O4-6 times, solution freeze-drying, produces sea lettuce albumen, this method in the solution can be by saturating using small-molecule substance in bag
Bag is analysed, and macromolecular substances can not reach removing (NH by the property of bag filter4)2SO4The purpose of small molecular weight impurity, operation letter
Single, effect is good, saves energy consumption;
Proteolysis:Sea lettuce albumen is pressed 1:4-6 solid-liquid ratios add water mixing, condition pH to 7-8, plus 1000-1200U/g pancreas egg
The papain of white enzyme enzyme and 600-800U/g, digests 6-8h at 45-50 DEG C, go out enzyme, cools down, centrifugation, and supernatant is
Enzymolysis liquid, the step prepares sea lettuce active peptides using double enzyme enzymatic isolation methods, using the selectivity of two kinds of albumen enzyme effects, makes raw material
Protein is fully hydrolyzed active polypeptides, improves the conversion ratio of sea lettuce protein, protein is degraded to activity as far as possible many
Peptide, and can make active peptide that there is delicious flavour, it is nutritious;
Ultrafiltration:The milipore filter ultrafiltration for being 3-5KDa with molecule interception by enzymolysis liquid, ultrafiltration pressure is 0.2-0.22MPa, is produced
Sea lettuce active peptides, its amino acid sequence is HSHSVCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR, the active peptides
With stronger antioxidation activity, with reference to the free radical and peroxide in cell metabolism, mitochondrial lipid peroxidation is prevented
Deng, protect cell resistance aging, promote UVB agings HSF propagation and collagen produce ability, can remarkably promote people into
The synthesis of collagen in fibrocyte, strengthens connective tissue, promotes cytoactive, so as to lift its elasticity, reduces wrinkle, increases
Strong gloss and transparency, the activity with anti-skin photoage activity are capable of the aging of delaying skin, available for cosmetics neck
Domain.
Compared with prior art, the advantage of the invention is that:The preparation method for the sea lettuce active peptides that the present invention is provided, system
Standby efficiency high, yield are high, purity is high, energy consumption is low, environmentally friendly, and technique is easy and effective, and production cost is low, and yield is big, is adapted to
Prepare sea lettuce active peptides on a large scale;Double enzyme enzymatic isolation methods that the preparation method is used are single-minded using two kinds of albumen enzyme effects
Property, material protein is fully hydrolyzed active polypeptides, improve the conversion ratio of sea lettuce protein, protein is degraded as far as possible
For active peptides;The active peptides that the extracting method is obtained have stronger antioxidation activity, with reference to the freedom in cell metabolism
Base and peroxide, prevent mitochondrial lipid peroxidation etc., protect cell resistance aging;The active peptides can promote UVB old
The ability that the HSF of change propagation and collagen is produced, can remarkably promote the synthesis of collagen in human fibroblasts, strengthen
Connective tissue, promotes cytoactive, so as to lift its elasticity, reduces wrinkle, enhancing gloss and transparency, old with anti-skin light
Change the activity of activity, be capable of the aging of delaying skin, available for cosmetic field.
Embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
The preparation method of sea lettuce active peptides, including clasmatosis, saltout, dialyse, proteolysis, ultrafiltration, specifically include following
Step:
1)Clasmatosis:Ulva lactuca powder is pressed 1:15 solid-liquid ratios add water mixing, are put into 4 DEG C of temperature, flow velocity 18mL/min, pressure
120MPa, circulate clasmatosis carried out in the cell crushing instrument of 3 times, filtering, by filtrate temperature be 45 DEG C, power be 200W's
Ultrasonication 30min, then the refrigerated centrifuge centrifugation 20min through 4 DEG C, 8000rpm, produces protein extract, the step
Breaking-wall cell is carried out with reference to cell crushing instrument and ultrasonic wave, the activity of protein is not influenceed, breaking-wall cell can be greatly shortened
Time, working effect is improved, frustule can be quantitatively crushed, it is adaptable to industrialized production;
2)Saltout:(NH is added into protein extract4)2SO4To 25% saturation degree, static 15min continuously adds (NH4)2SO4Extremely
Then 55% saturation degree, static 20min centrifuges 25min under 4 DEG C, 5000rpm, produces salt precipitation thing, is added in the step
Substantial amounts of ammonium sulfate, protein is obtained solubility reduces and Precipitation, and this method advantage is that ammonium sulfate concentrations change has
Continuity, effect of saltouing is good, saves energy consumption, and using the ammonium sulfate of various concentrations, can shorten the sedimentation time, effect is good
It is good;
3)Dialysis:Salt precipitation thing is dissolved in phosphate buffer, bag filter is then charged into, in ddH2Dialyse, change in O
ddH2O 5 times, solution is freeze-dried in bag, produces sea lettuce albumen, this method in the solution can be by saturating using small-molecule substance
Bag is analysed, and macromolecular substances can not reach removing (NH by the property of bag filter4)2SO4The purpose of small molecular weight impurity, operation letter
Single, effect is good, saves energy consumption;
4)Proteolysis:Sea lettuce albumen is pressed 1:5 solid-liquid ratios add water mixing, adjust pH to 7.5, plus 1200U/g trypsase
The papain of enzyme and 700U/g, 8h is digested at 45 DEG C, is gone out enzyme 16min in 100 DEG C of water-baths, is cooled to room temperature, 4 DEG C,
25min is centrifuged under 5000rpm, supernatant is enzymolysis liquid, the step prepares sea lettuce active peptides using double enzyme enzymatic isolation methods, utilizes
The selectivity of two kinds of albumen enzyme effects, makes material protein be fully hydrolyzed active polypeptides, improves the conversion ratio of sea lettuce protein,
Protein is degraded to active peptides as far as possible, and can make active peptide that there is delicious flavour, it is nutritious;
5)Ultrafiltration:The milipore filter ultrafiltration for being 3KDa with molecule interception by enzymolysis liquid, ultrafiltration pressure is 0.2MPa, produces sea lettuce work
Property polypeptide, its amino acid sequence be HSHSVCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR, the active peptides have compared with
Strong antioxidation activity, with reference to the free radical and peroxide in cell metabolism, prevents mitochondrial lipid peroxidation etc., protection
Cell resistance aging, the ability for promoting the HSF of UVB agings propagation and collagen to produce, can remarkably promote human desmocyte thin
The synthesis of collagen in born of the same parents, strengthens connective tissue, promotes cytoactive, so as to lift its elasticity, reduces wrinkle, strengthens gloss
With transparency, the activity with anti-skin photoage activity is capable of the aging of delaying skin, available for cosmetic field.
Embodiment 2:
The preparation method of sea lettuce active peptides, including clasmatosis, saltout, dialyse, proteolysis, ultrafiltration, specifically include following
Step:
1)Ulva lactuca powder is pressed 1:14 solid-liquid ratios add water mixing, are put into 4 DEG C of temperature, flow velocity 21mL/min, pressure 130MPa, circulation 2
Carry out clasmatosis in secondary cell crushing instrument, filtering, by filtrate in the ultrasonication that temperature is 50 DEG C, power is 200W
35min, then produces protein extract, the step combination cell crushing instrument carries out cell with ultrasonic wave through refrigerated centrifuge centrifugation
Broken wall, the activity of protein is not influenceed, can greatly shorten the time of breaking-wall cell, is improved working effect, can quantitatively be broken
Broken frustule, it is adaptable to industrialized production;
2)Saltout:(NH is added into protein extract4)2SO4To 22% saturation degree, static 18min continuously adds (NH4)2SO4Extremely
52% saturation degree, static 16min, centrifugation produces salt precipitation thing, substantial amounts of ammonium sulfate is added in the step, obtains protein molten
Xie Du is reduced and Precipitation, and this method advantage is that ammonium sulfate concentrations change has continuity, and effect of saltouing is good, saves energy
Consumption, and using the ammonium sulfate of various concentrations, the sedimentation time can be shortened, worked well;
3)Dialysis:Salt precipitation thing is dissolved in phosphate buffer, bag filter is then charged into, in ddH2Dialyse, change in O
ddH2O 4 times, solution is freeze-dried in bag, produces sea lettuce albumen, this method in the solution can be by saturating using small-molecule substance
Bag is analysed, and macromolecular substances can not reach removing (NH by the property of bag filter4)2SO4The purpose of small molecular weight impurity, operation letter
Single, effect is good, saves energy consumption;
4)Proteolysis:Sea lettuce albumen is pressed 1:4 solid-liquid ratios add water mixing, adjust pH to 7.8, plus 1100U/g trypsase
The papain of enzyme and 800U/g, 8h is digested at 45 DEG C, and go out enzyme, is cooled down, centrifugation, and supernatant is enzymolysis liquid, the step
Sea lettuce active peptides are prepared using double enzyme enzymatic isolation methods, using the selectivity of two kinds of albumen enzyme effects, make the abundant water of material protein
Active polypeptides are solved, the conversion ratio of sea lettuce protein is improved, protein is degraded to active peptides as far as possible, and activity can be made
Peptide has the advantages that delicious flavour, nutritious;
5)Ultrafiltration:The milipore filter ultrafiltration for being 4KDa with molecule interception by enzymolysis liquid, ultrafiltration pressure is 0.21MPa, produces sea lettuce
Active peptides, its amino acid sequence is HSHSVCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR, and the active peptides have
Stronger antioxidation activity, with reference to the free radical and peroxide in cell metabolism, prevents mitochondrial lipid peroxidation etc., protects
Cell resistance aging is protected, the ability for promoting the HSF of UVB agings propagation and collagen to produce can remarkably promote human desmocyte
The synthesis of collagen in cell, strengthens connective tissue, promotes cytoactive, so as to lift its elasticity, reduces wrinkle, strengthens light
Pool and transparency, the activity with anti-skin photoage activity, are capable of the aging of delaying skin, available for cosmetic field.
Embodiment 3:
The preparation method of sea lettuce active peptides:Ulva lactuca powder is pressed 1:16 solid-liquid ratios add water mixing, are put into 6 DEG C of temperature, flow velocity 20mL/
Min, pressure 110MPa, circulate clasmatosis is carried out in the cell crushing instrument of 3 times, filtrate in temperature is 48 DEG C, power by filtering
For 220W ultrasonication 30min, centrifugation;(NH is added into centrifugate4)2SO4To 25% saturation degree, static 18min continues
Add (NH4)2SO4To 53% saturation degree, static 17min, centrifugation produces salt precipitation thing;Salt precipitation thing is dissolved in phosphoric acid
Salt buffer, is then charged into bag filter, in ddH2Dialysed in O, change ddH2O 6 times, solution is freeze-dried in bag, produces sea lettuce
Albumen;Sea lettuce albumen is pressed 1:4 solid-liquid ratios add water mixing, adjust pH to 7.8, plus 1200U/g trypsase enzyme and 800U/g
Papain, digest 6h at 50 DEG C, go out enzyme, cool down, centrifugation;The milipore filter for being 5KDa with molecule interception by supernatant
Ultrafiltration, ultrafiltration pressure is 0.2MPa, produces sea lettuce active peptides.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Zhoushan Fu Sheng food science and technologies Co., Ltd
<120>The preparation method of sea lettuce active peptides
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 39
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ser Val Cys Gly Tyr Tyr Val Ser His Arg Gly Arg Cys
1 5 10 15
Tyr Cys Arg Cys Leu Arg Cys Arg Val Leu His Pro Gly Lys Leu Cys
20 25 30
Val Cys Val Asn Cys Ser Arg
35
Claims (10)
1. the preparation method of sea lettuce active peptides, including clasmatosis, saltout, dialyse, proteolysis, ultrafiltration, it is characterised in that:
The ultrafiltration is:The milipore filter for being 3-5KDa with molecule interception by enzymolysis liquid, the ultrafiltration in the case where pressure is 0.2-0.22MPa, i.e.,
Obtain sea lettuce active peptides.
2. the preparation method of sea lettuce active peptides according to claim 1, it is characterised in that:Sea lettuce activity in the ultrafiltration
The amino acid sequence of polypeptide is HSHSVCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.
3. the preparation method of sea lettuce active peptides according to claim 1, it is characterised in that:The clasmatosis is:Will
Ulva lactuca powder adds water mixing, is put into cell crushing instrument and carries out clasmatosis, filtering, and by filtrate ultrasonication, centrifugation produces egg
White extract solution.
4. the preparation method of sea lettuce active peptides according to claim 3, it is characterised in that:Sea lettuce in the clasmatosis
Powder is 1 with the solid-liquid ratio said:4-6.
5. the preparation method of sea lettuce active peptides according to claim 3, it is characterised in that:Cell in the clasmatosis
The parameter of broken instrument is:Temperature is 4-6 DEG C, and flow velocity is 18-21mL/min, and pressure is 110-130MPa, is circulated 2-3 times.
6. the preparation method of sea lettuce active peptides according to claim 3, it is characterised in that:It is ultrasonic in the clasmatosis
Ripple treatment temperature is 40-50 DEG C, and power is 200-300W, and the time is 25-35min.
7. the preparation method of sea lettuce active peptides according to claim 1, it is characterised in that:It is described saltout in add first
(NH4)2SO4To 20-25% saturation degrees, secondary addition (NH4)2SO4To 50-55% saturation degrees.
8. the preparation method of sea lettuce active peptides according to claim 1, it is characterised in that:Sea lettuce in the proteolysis
The solid-liquid ratio of albumen and water is 1:14-16.
9. the preparation method of sea lettuce active peptides according to claim 1, it is characterised in that:Pancreas egg in the proteolysis
The addition of white enzyme enzyme is 1000-1200U/g, and the addition of papain is 600-800U/g.
10. the preparation method of sea lettuce active peptides according to claim 1, it is characterised in that:Enzyme in the proteolysis
Solution pH is 7-8, and temperature is 45-50 DEG C, and the time is 6-8h.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108185267A (en) * | 2017-12-28 | 2018-06-22 | 广州市科能化妆品科研有限公司 | A kind of anti-oxidation peptide composition and its preparation method and application |
CN108977484A (en) * | 2018-05-17 | 2018-12-11 | 金华市艾力生物科技有限公司 | The extracting method of oat polypeptide |
CN109852656A (en) * | 2019-03-21 | 2019-06-07 | 大洲新燕(厦门)生物科技有限公司 | A kind of bird's nest polypeptide powder and preparation method thereof |
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CN102154423A (en) * | 2011-01-12 | 2011-08-17 | 淮海工学院 | Method for preparing active peptide of laver |
KR20130012426A (en) * | 2011-07-25 | 2013-02-04 | 포항공과대학교 산학협력단 | Method for the production of d-glucaric acid from green seaweed |
CN106119327A (en) * | 2016-06-29 | 2016-11-16 | 大连深蓝肽科技研发有限公司 | The method that oligopeptide makes the oligomeric Gly-His-Lys of Sargassum is extracted from Sargassum |
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102154423A (en) * | 2011-01-12 | 2011-08-17 | 淮海工学院 | Method for preparing active peptide of laver |
KR20130012426A (en) * | 2011-07-25 | 2013-02-04 | 포항공과대학교 산학협력단 | Method for the production of d-glucaric acid from green seaweed |
CN106119327A (en) * | 2016-06-29 | 2016-11-16 | 大连深蓝肽科技研发有限公司 | The method that oligopeptide makes the oligomeric Gly-His-Lys of Sargassum is extracted from Sargassum |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108185267A (en) * | 2017-12-28 | 2018-06-22 | 广州市科能化妆品科研有限公司 | A kind of anti-oxidation peptide composition and its preparation method and application |
CN108977484A (en) * | 2018-05-17 | 2018-12-11 | 金华市艾力生物科技有限公司 | The extracting method of oat polypeptide |
CN109852656A (en) * | 2019-03-21 | 2019-06-07 | 大洲新燕(厦门)生物科技有限公司 | A kind of bird's nest polypeptide powder and preparation method thereof |
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