CN107266554A - 制备重组血管扩张肽的方法、表达构建体、宿主细胞及重组融合多肽 - Google Patents

制备重组血管扩张肽的方法、表达构建体、宿主细胞及重组融合多肽 Download PDF

Info

Publication number
CN107266554A
CN107266554A CN201710409638.4A CN201710409638A CN107266554A CN 107266554 A CN107266554 A CN 107266554A CN 201710409638 A CN201710409638 A CN 201710409638A CN 107266554 A CN107266554 A CN 107266554A
Authority
CN
China
Prior art keywords
peptide
vsdl
cleavage site
leu
pro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710409638.4A
Other languages
English (en)
Chinese (zh)
Inventor
斯坦·巴斯蒂拉斯
安吉洛·圭多林
本·亨特
赛义夫·莱希德
雷扎·扎雷尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Madeleine Pharmaceuticals Pty Ltd
Original Assignee
Madeleine Pharmaceuticals Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Madeleine Pharmaceuticals Pty Ltd filed Critical Madeleine Pharmaceuticals Pty Ltd
Publication of CN107266554A publication Critical patent/CN107266554A/zh
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/58Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Cardionatrin; Cardiodilatin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/06Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cardiology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201710409638.4A 2012-03-19 2013-03-19 制备重组血管扩张肽的方法、表达构建体、宿主细胞及重组融合多肽 Pending CN107266554A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261612817P 2012-03-19 2012-03-19
US61/612,817 2012-03-19
CN201380025049.6A CN104364378A (zh) 2012-03-19 2013-03-19 制备重组肽的方法

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201380025049.6A Division CN104364378A (zh) 2012-03-19 2013-03-19 制备重组肽的方法

Publications (1)

Publication Number Publication Date
CN107266554A true CN107266554A (zh) 2017-10-20

Family

ID=49221695

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201710409638.4A Pending CN107266554A (zh) 2012-03-19 2013-03-19 制备重组血管扩张肽的方法、表达构建体、宿主细胞及重组融合多肽
CN201380025049.6A Pending CN104364378A (zh) 2012-03-19 2013-03-19 制备重组肽的方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201380025049.6A Pending CN104364378A (zh) 2012-03-19 2013-03-19 制备重组肽的方法

Country Status (8)

Country Link
US (1) US20150315259A1 (enExample)
EP (1) EP2839010B1 (enExample)
JP (1) JP2015510770A (enExample)
KR (1) KR20140148420A (enExample)
CN (2) CN107266554A (enExample)
AU (2) AU2013234860B2 (enExample)
SG (1) SG11201405721VA (enExample)
WO (1) WO2013138850A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8947194B2 (en) 2009-05-26 2015-02-03 Solaredge Technologies Ltd. Theft detection and prevention in a power generation system
EP3773669A4 (en) * 2018-03-29 2022-04-27 Contrafect Corporation ANTIMICROBIAL BACTERIOPHAGE-DERIVED POLYPEPTIDES AND THEIR USE AGAINST GRAM-NEGATIVE BACTERIA
CN113121637B (zh) * 2020-01-15 2022-06-14 鲁南制药集团股份有限公司 一种重组蛋白的分离纯化方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5691310A (en) * 1987-09-29 1997-11-25 Vesely; David L. Methods of treatment using proANF peptides
CN1313896A (zh) * 1998-07-10 2001-09-19 赛欧斯公司 生产等电点高于8或低于5的多肽的方法
WO2002002591A2 (en) * 2000-06-30 2002-01-10 Becton, Dickinson And Company Peptides for use in culture media
US6558924B1 (en) * 1997-08-07 2003-05-06 Creative Peptides Sweden Ab Recombinant expression of insulin C-peptide

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000017336A1 (en) * 1998-09-19 2000-03-30 Sang Jun Lee Dna cassette encoding a multimer of a biologically active peptide and a cleavable linker attached thereto and process for preparing the biologically active peptide
CN1363654A (zh) * 2001-07-19 2002-08-14 上海华谊生物技术有限公司 生产促胰岛素分泌肽glp-1(7-36)的基因工程菌以及生产glp-1(7-36)的方法
ES2343061T3 (es) * 2002-05-24 2010-07-22 Medtronic, Inc. Metodos y constructos de dna para produccion de polipeptidos con rendimiento alto.
DE60331584D1 (de) * 2002-11-26 2010-04-15 Biocon Ltd Modifizierte natriuretic verbindungen, konjugate und ihre verwendungen
WO2005094420A2 (en) * 2004-02-17 2005-10-13 University Of South Florida Materials and methods for treatment of inflammatory and cell proliferation disorders
EP1736480B9 (en) * 2005-06-02 2009-03-04 Polyquant GmbH Artificial protein, method for absolute quantification of proteins and uses thereof
JP5395794B2 (ja) * 2007-09-11 2014-01-22 モンドバイオテック ラボラトリーズ アクチエンゲゼルシャフト 治療剤としてのガラニンペプチドの使用
RU2451750C2 (ru) * 2007-09-24 2012-05-27 Общество С Ограниченной Ответственностью "Герофарм" Способ получения рекомбинантного с-пептида проинсулина человека
US8527211B2 (en) * 2008-06-18 2013-09-03 Reza Zareie Method to produce recombinant MBP8298 and other polypeptides by nucleotide structure optimization
JP2013505013A (ja) * 2009-09-18 2013-02-14 セレクシス エス.エー. 強化導入遺伝子発現およびプロセッシングの産物および方法
WO2012019237A1 (en) * 2010-08-12 2012-02-16 Madeleine Pharmaceuticals Pty Ltd Therapeutic method for treating congestive heart failure
WO2012115772A2 (en) * 2011-02-25 2012-08-30 Medtronic, Inc. Therapy for kidney disease and/or heart failure

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5691310A (en) * 1987-09-29 1997-11-25 Vesely; David L. Methods of treatment using proANF peptides
US6558924B1 (en) * 1997-08-07 2003-05-06 Creative Peptides Sweden Ab Recombinant expression of insulin C-peptide
CN1313896A (zh) * 1998-07-10 2001-09-19 赛欧斯公司 生产等电点高于8或低于5的多肽的方法
WO2002002591A2 (en) * 2000-06-30 2002-01-10 Becton, Dickinson And Company Peptides for use in culture media

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
VESELY, DL等: "Circadian relationships between circulating atrial natriuretic peptides and serum calcium and phosphate in healthy humans", 《METABOLISM》 *
徐小寅: "Alloferon-1抗菌肽串联式重组表达及重组Alloferon-1生物学活性研究", 《医药卫生科技辑》 *
李竞超等: "斑马鱼ANF基因的克隆及多克隆抗体的制备", 《激光生物学报》 *
赵明等: "在大肠杆菌中表达可溶的多串心钠素", 《中国生物化学与分子生物学报》 *

Also Published As

Publication number Publication date
CN104364378A (zh) 2015-02-18
EP2839010B1 (en) 2018-04-25
AU2016100212A4 (en) 2016-04-14
EP2839010A4 (en) 2015-12-09
KR20140148420A (ko) 2014-12-31
EP2839010A1 (en) 2015-02-25
JP2015510770A (ja) 2015-04-13
US20150315259A1 (en) 2015-11-05
WO2013138850A1 (en) 2013-09-26
AU2013234860A1 (en) 2014-10-16
SG11201405721VA (en) 2014-10-30
AU2013234860B2 (en) 2016-01-21

Similar Documents

Publication Publication Date Title
EP3746466B1 (en) Manufacture of glucagon peptides
CN104619726B (zh) 由超折叠绿色荧光蛋白构成的融合蛋白及其用途
EP2787083B1 (en) Efficient production of peptides
CA2589951C (en) Method for producing carboxy-terminal amidated peptides
CA2623547C (en) Method for amidating polypeptides with basic aminoacid c-terminals by means of specific endoproteases
CN110724187B (zh) 一种高效表达利拉鲁肽前体的重组工程菌及其应用
US20140058790A1 (en) Deuterated peptides
CN107266554A (zh) 制备重组血管扩张肽的方法、表达构建体、宿主细胞及重组融合多肽
CN113614113B (zh) 含有荧光蛋白片段的融合蛋白及其用途
WO2021147869A1 (zh) 利拉鲁肽衍生物及其制备方法
KR102345011B1 (ko) GroES 융합을 이용한 글루카곤 유사 펩타이드-1 또는 이의 유사체의 생산방법
WO2021249564A1 (zh) 一种索玛鲁肽衍生物及其制备方法和应用
US20090035815A1 (en) Synthetic Gene for Enhanced Expression in E. Coli
KR102345013B1 (ko) GroES 융합을 이용한 글루카곤 유사 펩타이드-2 또는 이의 유사체의 생산방법

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20171020

WD01 Invention patent application deemed withdrawn after publication