CN107259141B - Feed additive for pregnant sows and preparation and use methods thereof - Google Patents

Feed additive for pregnant sows and preparation and use methods thereof Download PDF

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CN107259141B
CN107259141B CN201710511498.1A CN201710511498A CN107259141B CN 107259141 B CN107259141 B CN 107259141B CN 201710511498 A CN201710511498 A CN 201710511498A CN 107259141 B CN107259141 B CN 107259141B
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CN107259141A (en
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文敏
陈清华
苗超
邹永忠
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Hunan Lvheng Shiyuan Animal Pharmaceutical Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/30Feeding-stuffs specially adapted for particular animals for swines
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/28Silicates, e.g. perlites, zeolites or bentonites

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  • Proteomics, Peptides & Aminoacids (AREA)
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Abstract

The invention discloses a feed additive for pregnant sows and a preparation and use method thereof. The additive is obtained by uniformly mixing the materials 1 part by weight of 1, 2 and 3 respectively with yeast cell wall polysaccharide according to the weight ratio of 10: 1; wherein the material 1 consists of 50 parts of lactobacillus plantarum metabolite antibacterial peptide and 50 parts of lactobacillus rhamnosus; the material 2 consists of 20 parts of glucose oxidase, 20 parts of catalase, 20 parts of peptide enzyme, 20 parts of epoxidase and 20 parts of phosphatase; the material No. 3 comprises 50 parts of calcareous montmorillonite, 15 parts of gypsum, 15 parts of anhydrous sodium sulphate and 20 parts of baking soda. The product of the invention is added into the feed of the sow according to 2 kg/ton in the 90 days of gestation and delivery period of the sow. The product of the invention can effectively prevent mycotoxin and vivotoxin from influencing pregnant sows, the litter size and healthy litter size of the sows are obviously improved, the farrowing time interval of the sows during farrowing is obviously shortened, the disease resistance of the sows is obviously improved, and the reproductive performance of the sows is obviously improved.

Description

Feed additive for pregnant sows and preparation and use methods thereof
Technical Field
The invention relates to a feed additive, in particular to a feed additive suitable for detoxifying, removing mildew, detoxifying and diminishing inflammation of pregnant sows (90 days of gestation-delivery stage), and a preparation and use method thereof.
Background
Mycotoxin contamination has become a major hazard affecting the development of the world agriculture, feed industry and breeding industry. How to recognize and control mycotoxin becomes the primary problem of solving the safety of feed and livestock products. The mould and the toxin thereof not only reduce the nutritive value and palatability of the feed and influence the storage, transportation and use of the feed, but also destroy the immune function and the oxidation-reduction steady state of an organism of an animal and reduce the disease resistance and the health level of the animal, thereby reducing the production performance of the animal and wasting feed resources. And acute and chronic poisoning of different symptoms of animals can be caused, the health and even life of the animals are damaged, and the influence on pigs is the greatest.
Mycotoxins are antinutritional factors present in feed and raw materials and are highly toxic secondary metabolites of molds. Mycotoxins are produced during processing, transport and storage of feed. Feed mycotoxins are ubiquitous and vary by as many as several tens. For example, aflatoxin has strong carcinogenicity and mainly destroys the liver of animals; gibberellin, like estrogen, destroys the reproductive organs of animals; t-2 toxin destroys animal immune organs, vomits toxin to influence pig appetite and causes damage to digestive organs. Meanwhile, when the biogenic amine generated by normal metabolism in the animal body, the antibiotics added in the feed and toxic and harmful substances such as endotoxin generated after the antibiotics kill intestinal bacteria are difficult to be completely decomposed, transformed and detoxified in the liver, the biogenic amine directly enters the blood to cause normal physiological dysfunction of the animal. The sows face environmental stress and a plurality of threat factors in feed in the gestation period, particularly after 90 days of gestation, the fetuses grow rapidly, 3/4 of the weight of the fetuses is completed in the period, the fetuses gain the burden of the liver and kidney excretion of the sows, and the liver and kidney functions of the sows are seriously damaged, so that the sows are frequently subjected to abortion, stillbirth, over-long labor process, dystocia, persistent lochia, postpartum fatigue paralysis and sleep, appetite disorder, low feed intake, insufficient milk, piglet milk-derived diarrhea, prolonged oestrus intervals and other symptoms. At present, mycotoxin adsorbents are commonly added into daily rations of sows to prevent the occurrence of the diseases or phenomena, so that the effects of relieving the diseases or phenomena can be achieved to a certain extent. However, the mycotoxin adsorbent in the market mainly uses a physical adsorbent, adsorbs a great amount of vitamins (especially fat-soluble vitamins), trace elements and other nutritional ingredients in the feed and excludes the mycotoxin from the body, meanwhile, effective adsorption is not complete, the mycotoxin adsorbent is ineffective for the swine herd with mycotoxin poisoning, and in addition, endotoxin (such as Lipopolysaccharide (LPS)) cannot be adsorbed and degraded and absorbed into the blood, so that the purposes and effects of true detoxification, detoxication and detoxification cannot be achieved. Based on the above, there is a need for a feed additive product suitable for detoxifying, removing mildew, detoxifying and diminishing inflammation of pregnant sows (90 days of gestation-delivery stage).
Disclosure of Invention
Aiming at the problems and frequently occurring diseases in the breeding link of the sows at present, the invention provides the feed additive for the pregnant sows (90 days of gestation-parturition stage) and the preparation method thereof, so as to thoroughly eliminate various toxins in the bodies, enhance the immunity of the sows, improve the number of live sows and improve the birth average weight of piglets.
In order to solve the technical problems, the invention adopts the technical scheme that the feed additive for the pregnant sows (90 days of gestation-the stage of delivery) is obtained by uniformly mixing 1 part of material 1, material 2 and material 3 in parts by weight and then uniformly mixing the mixture with yeast cell wall polysaccharide in a weight ratio of 10: 1. Wherein the material 1 is antibacterial peptide (titer is more than or equal to 10) derived from Lactobacillus plantarum metabolite4U/g)50 parts, lactobacillus rhamnosus (the content is more than or equal to 10)7CFU/g)50 parts; the material 2 consists of 20 parts of glucose oxidase (the enzyme activity content is more than or equal to 1000U/g), 20 parts of catalase (the enzyme activity content is more than or equal to 1000U/g), 20 parts of peptide enzyme (the enzyme activity content is more than or equal to 1000U/g), 20 parts of cyclooxido reductase (the enzyme activity content is more than or equal to 1000U/g) and 20 parts of phosphatase (the enzyme activity content is more than or equal to 10000U/g); the material No. 3 comprises 50 parts of calcium montmorillonite which contains more than 5 percent of opal and is calcined and expanded at the high temperature of 380 ℃, 15 parts of gypsum, 15 parts of anhydrous sodium sulphate and 20 parts of baking soda.
The specific process of the invention comprises the following steps:
1) taking antibacterial peptide (titer is more than or equal to 10) of lactobacillus plantarum metabolite4U/g)50 parts, lactobacillus rhamnosus (the content is more than or equal to 10)7CFU/g)50 parts, uniformly mixing, controlling the water content not to exceed 10 percent, and taking the mixture as a material 1;
2) taking 20 parts of glucose oxidase (the enzyme activity content is more than or equal to 1000U/g), 20 parts of catalase (the enzyme activity content is more than or equal to 1000U/g), 20 parts of peptide-forming enzyme (the enzyme activity content is more than or equal to 1000U/g), 20 parts of cycloxygenase (the enzyme activity content is more than or equal to 1000U/g) and 20 parts of phosphatase (the enzyme activity content is more than or equal to 10000U/g), uniformly mixing, controlling the water content to be less than 8 percent, and taking the mixture as a material No. 2;
3) 50 parts of calcareous montmorillonite which contains more than 5 percent of opal and is calcined and expanded at the high temperature of 380 ℃, 15 parts of gypsum, 15 parts of anhydrous sodium sulphate and 20 parts of baking soda are uniformly mixed, crushed and sieved by a 40-mesh sieve, and the crushed mixture is used as a material No. 3 with the water content not exceeding 5 percent.
4) Mixing material No. 1, material No. 2 and material No. 3 at a weight ratio of 10:1 with yeast cell wall polysaccharide (containing beta-glucan more than or equal to 30% and mannan-oligosaccharide more than or equal to 20%) to obtain the final product, wherein the water content is controlled not to exceed 12%.
The application method of the feed additive for pregnant sows, which is prepared by the invention, comprises the following steps: the feed is added into the feed of the sows according to the standard of adding 2000 g of complete formula feed per ton of sows in the 90-day gestation and delivery period of the sows, and the sows are used after being uniformly mixed.
The feed additive of the invention has the following advantages:
1) the invention integrates biological enzymolysis technology, utilizes the effects of esterase for degrading zearalenone and fumonisin feed, epoxidase for degrading T-2 and vomitoxin, peptide enzyme for degrading ochratoxin and the like, and is reasonably matched to achieve the purpose of thoroughly removing mycotoxin in intestinal tracts.
2) Adopts the compatibility of glucose oxidase and catalase to eliminate various toxic substances such as free radicals in vivo and hydrogen peroxide to oxidize vivotoxin and the like, so that toxic substances in the sow body are changed into nontoxic substances, and H is converted into H2O2Further converted into nontoxic H2O, is beneficial to protecting the liver and the kidney. Meanwhile, O is consumed in the process of catalyzing glucose by glucose oxidase2The product can generate gluconic acid and hydrogen peroxide, so that the digestive tract is easier to form an anaerobic environment, the proliferation of anaerobic beneficial bacteria is promoted, the functions of acidifier and probiotics are achieved, the acidic digestive environment of the digestive tract can be improved, the integrity of epithelial cells of the intestinal tract is protected, and the intestinal tract having the acidic digestive environment can be inhibitedAnd (4) proliferating harmful microorganisms and adjusting the ecological balance of intestinal flora.
3) The increase of the body surface area and the change of the molecular structure of the montmorillonite containing the opal in the calcining and expanding process are utilized to enhance the performance of adsorbing toxin in the feed and endotoxin (such as Lipopolysaccharide (LPS)) generated by harmful microorganisms in the body, and other nutrient components in the feed are not adsorbed, so that the intestinal toxin is thoroughly purified.
4) The selected yeast cell wall polysaccharide contains beta-glucan more than or equal to 30 percent and mannan-oligosaccharide more than or equal to 20 percent, has an efficient immune activation function, can improve the nonspecific immunity level of pregnant sows, improve the liver function, improve the activity of immune related enzymes, and can relieve various stress reactions of the pregnant sows; has strong adsorption effect on mycotoxin and can inhibit colonization of pathogenic bacteria in intestinal tract; the effective components of the feed additive are absorbed and then combined with mycotoxin which is absorbed into blood by animals, and the toxin is transported into the liver for detoxification and then is discharged out of the body, so that the absorbed toxin can be removed, and the traditional mildew removing agent cannot remove the toxin which is absorbed by the body out of the body.
5) The invention adopts the compatibility of the antibacterial peptide produced by lactobacillus plantarum fermentation and lactobacillus rhamnosus, fully adjusts the microecological balance of the digestive tract of the sow, prevents the propagation of pathogenic bacteria through an adhesion mechanism and a competitive exclusion action, inhibits pathogenic microorganisms, establishes and maintains a normal intestinal dominant population, and prevents the occurrence of diseases of the sow. Improving intestinal tract motility of sow, eliminating constipation of sow, reducing toxin accumulation, and reducing weak fetus and stillbirth. The antibacterial peptide has the functions of sterilization and bacteriostasis, and reduces the vertical infection of the sow.
6) In order to better clear heat, diminish inflammation and detoxify, the product comprehensively uses gypsum, anhydrous sodium sulphate, sodium bicarbonate and other inorganic mineral substances, and is compounded according to a certain ratio, so that the exertion of the effects of the compatible raw materials is promoted, the aim of synergistically regulating the normal metabolism of the pregnant sow in a special physiological stage is fulfilled, the normal ingestion of the pregnant sow is promoted, and the digestive function of the sow is improved.
Detailed Description
Example 1:
1) taking antibacterial peptide (titer is more than or equal to 10) of lactobacillus plantarum metabolite4U/g)50 portions of lactobacillus rhamnosus (the content is more than or equal to 10)7CFU/g)50 parts, uniformly mixing, controlling the water content not to exceed 10 percent, and taking the mixture as a material 1;
2) taking 20 parts of glucose oxidase (the enzyme activity content is more than or equal to 1000U/g) produced by aspergillus niger, 20 parts of catalase (the enzyme activity content is more than or equal to 1000U/g) produced by penicillium, 20 parts of peptide-growing enzyme (the enzyme activity content is more than or equal to 1000U/g) produced by bacillus licheniformis, 20 parts of epoxidoreductase (the enzyme activity content is more than or equal to 1000U/g) produced by aspergillus niger and 20 parts of phosphatase (the enzyme activity content is more than or equal to 10000U/g) produced by aspergillus niger, uniformly mixing, controlling the water content to be less than or equal to 8 percent, and taking the mixture as a;
3) 50 parts of calcium montmorillonite which contains more than 5 percent of opal and is calcined and expanded at the high temperature of 380 ℃, 15 parts of gypsum (natural calcium sulfate dihydrate with the purity of more than or equal to 98.0 percent), 15 parts of anhydrous sodium sulfate (sodium sulfate of more than or equal to 99.0 percent) and 20 parts of baking soda (sodium bicarbonate of more than or equal to 99.0 percent) are taken, mixed uniformly, crushed and sieved by a 40-mesh sieve, and the water content is controlled to be less than or equal to 5 percent to be used as a material No. 3.
4) Mixing material No. 1, material No. 2 and material No. 3 at a weight ratio of 10:1 with yeast cell wall polysaccharide (containing beta-glucan more than or equal to 30% and mannan-oligosaccharide more than or equal to 20%) to obtain the final product, wherein the water content is controlled not to exceed 12%.
The application method of the feed additive for pregnant sows, which is prepared by the invention, comprises the following steps: the feed is added into the feed of the sows according to the standard of adding 2000 g of complete formula feed per ton of sows in the 90-day gestation and delivery period of the sows, and the sows are used after being uniformly mixed.
The beneficial effects of the feed additive for pregnant sows (90 days of gestation-stage of delivery) according to the present invention are illustrated by the following specific test examples:
test of the Effect of a feed additive for pregnant sows (90 days of gestation-stage of delivery) on the Productivity of pregnant sows
Selecting 40 large white sows with good health status of 3-5 births and 88-90d pregnancies, randomly dividing into a control group and a test group according to the age of mating days, the births and the body conditions, treating 20 replicates each, and repeating 1 sow each. By adopting single-factor experimental design, the basic ration is the same, and the main nutrient components are as follows: digestion energy of 3000Mcal/kg, crude protein 13.00%, lysine 0.48%, methionine 0.12%, threonine 0.38%, tryptophan 0.10%, calcium 0.68%, and available phosphorus 0.32%. The pregnant sows are raised in a limit fence, and transferred to an overhead obstetric table 1 week before delivery. The control group is fed with basic daily ration, and the feed additive for pregnant sows is added into the feed for the sows of the test group according to 2kg of the feed per ton of the basic daily ration. The test pigs have consistent environmental conditions. The test time is 2016, 10 months, 20 days to 11 months and 22 days, and the site is a certain original pig farm in Hunan. The sow can freely drink water, and the environment is well cleaned and sanitary according to the conventional health care immunity.
The measurement indexes are as follows: average daily feed intake of sows, farrowing time interval of sows, birth weight of piglets, live rate, healthy litter size, dead and mummy rate, malformation and weak litter size of piglets, and recording the midwifery condition of sows and the occurrence condition of postpartum diseases. The influence of the feed additive for pregnant sows on the average daily feed intake of the sows is shown in table 1, the influence on the productivity index of the sows is shown in table 2, and the influence on the prevention and the midwifery condition of postpartum diseases of the sows is shown in table 3.
TABLE 1 influence of feed additives for pregnant sows on average daily feed intake of sows (kg/day. head)
Phases Test group (20 heads) Control group (20 heads)
30 days before delivery to 4 days before delivery 6.12±0.41a 5.87±0.18a
3 days before delivery-postpartum 1.35±0.10a 1.24±0.10a
Note: the same row data with different shoulder letters shows significant difference (P <0.05), and the same data with different letters shows no significant difference (P > 0.05). The following table is the same.
TABLE 2 influence of feed additives for pregnant sows on the performance index of sows
Index (I) Test group (20 heads) Control group (20 heads)
Farrowing time interval (min) 18.03±4.23a 31.11±5.49b
Average number of live piglets (head) 11.00±2.35a 8.70±1.52b
Average birth weight (kg) of young animals 1.47±0.28a 1.42±0.35a
Percentage of healthy birth (%) 97.92a 90.91b
Dead tires and mummyPercentage (%) 0.00a 2.28b
Deformity and weak rate (%) 0.45a 4.59b
TABLE 3 influence of feed additives for pregnant sows on prevention of postpartum diseases and midwifery of sows
Treatment of Test group (20 heads) Control group (20 heads)
Postpartum disease condition of sow 0 head Fever after 2 postpartum 6 hours and 3 postpartum metritis
Incidence of disease 0% 25.00%
Midwifery condition of sow 0 head 2 heads midwifery
Yield-promoting rate 0% 10.00%
The results show that: compared with the control group, the feed intake of the sows and the average birth weight of the piglets of the test group tend to increase, but the difference is not significant (P is more than 0.05). The farrowing interval time of the sows in the test group is obviously shorter than that of the control group, the farrowing number and the healthy farrowing number are obviously higher than those of the control group, the stillbirth and mummy rate, the malformation and weak farrowing rate are obviously lower than those of the control group (P <0.05), the sows in the test group do not need to be midwife, have no postpartum diseases and are obviously better than those of the control group.
It is thus known that: the feed additive for the pregnant sows (90 days of gestation-childbirth stage) has certain effects on the productivity and disease resistance of the pregnant sows. The additive is added into feed at the stage of delivery of 90 days of gestation sows, which is 2kg/t, so that the litter size and healthy litter size of the sows can be effectively increased, the farrowing time interval of the sows is remarkably shortened, the disease resistance of the sows is remarkably improved, and the reproductive performance of the sows is improved.

Claims (3)

1. A feed additive for pregnant sows is characterized in that 1 part of material 1, material 2 and material 3 are uniformly mixed according to the parts by weight and then uniformly mixed with yeast cell wall polysaccharide containing more than or equal to 30 percent of beta-glucan and more than or equal to 20 percent of mannan oligosaccharide according to the weight ratio of 10:1 to obtain the feed additive; wherein the titer of the material 1 is more than or equal to 10450 parts of U/g lactobacillus plantarum metabolite antibacterial peptide, with the content being more than or equal to 10750 parts of CFU/g lactobacillus rhamnosus; the material 2 consists of 20 parts of glucose oxidase with the enzyme activity content of more than or equal to 1000U/g, 20 parts of catalase with the enzyme activity content of more than or equal to 1000U/g, 20 parts of peptide enzyme with the enzyme activity content of more than or equal to 1000U/g, 20 parts of epoxidase with the enzyme activity content of more than or equal to 1000U/g and 20 parts of phosphatase with the enzyme activity content of more than or equal to 10000U/g; the material 3 consists of 50 parts of calcareous montmorillonite with the content of the opal not less than 5 percent and calcined at the high temperature of 380 ℃, 15 parts of gypsum, 15 parts of anhydrous sodium sulphate and 20 parts of baking soda.
2. A preparation method of a feed additive for pregnant sows is characterized by comprising the following steps:
1) the titer is more than or equal to 10450 parts of U/g lactobacillus plantarum metabolite antibacterial peptide, with the content being more than or equal to 10750 parts of CFU/g lactobacillus rhamnosus, uniformly mixing, controlling the water content to be less than or equal to 10 percent, and taking the mixture as a material 1;
2) taking 20 parts of glucose oxidase with the enzyme activity content of more than or equal to 1000U/g, 20 parts of catalase, 20 parts of peptide enzyme, 20 parts of epoxidase and 20 parts of phosphatase with the enzyme activity content of more than or equal to 10000U/g, uniformly mixing, and controlling the water content to be less than or equal to 8 percent to be used as a material No. 2;
3) 50 parts of calcium montmorillonite, 15 parts of gypsum, 15 parts of anhydrous sodium sulphate and 20 parts of baking soda, wherein the content of the calcium montmorillonite is more than or equal to 5 percent and is calcined and expanded at the high temperature of 380 ℃, the calcium montmorillonite, the gypsum, the anhydrous sodium sulphate and the baking soda are uniformly mixed, crushed and sieved by a 40-mesh sieve, and the water content is controlled to be less than or equal to 5 percent to be used as a material No. 3;
4) and (2) uniformly mixing the material No. 1, the material No. 2 and the material No. 3 in parts by weight, uniformly mixing the mixture with the yeast cell wall polysaccharide according to the weight ratio of 10:1, and controlling the water content to be less than or equal to 12 percent to obtain the yeast cell wall polysaccharide, wherein the content of beta-glucan in the yeast cell wall polysaccharide is more than or equal to 30 percent, and the content of mannan oligosaccharide is more than or equal to 20 percent.
3. Use of the feed additive for pregnant sows as claimed in claim 1 or 2 in the preparation of feed for sows, characterized in that: 2000 g of the feed additive is added into the complete compound feed for each ton of sows and is mixed evenly.
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