CN107255715A - A kind of microorganism detection system and method - Google Patents
A kind of microorganism detection system and method Download PDFInfo
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- CN107255715A CN107255715A CN201710642147.4A CN201710642147A CN107255715A CN 107255715 A CN107255715 A CN 107255715A CN 201710642147 A CN201710642147 A CN 201710642147A CN 107255715 A CN107255715 A CN 107255715A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
Abstract
The invention provides a kind of microorganism detection system and method, a mobile test end is designed first to be acquired the test result of micro-biological samples and microbial antibodies test solution, temperature now is measured simultaneously, relative humidity and air pressure, then by the test result, temperature, relative humidity and pressure information are by being sent to data terminal, data terminal is by the information of reception from testing obtained different temperature in advance, test experience result under varying environment relative humidity and different air pressure conditions is contrasted, obtain the analysis result of microorganism, and the analysis result is sent to mobile test end, solving existing microorganism detection technology needs to carry out in laboratory conditions, the problem of testing result time lag and low detection efficiency, reach and simplified detection method, increase detection device portability and improve the purpose of detection accuracy.
Description
Technical field
The invention belongs to field of measuring technique, it is related to a kind of microorganism detection system and method.
Background technology
Micro organism quantity in food, usually as the mark for judging food microbial contamination degree in food hygiene
Will, also can as observation microorganism in food property and the dynamic that be bred in food of microorganism, so as to being detected
Food carries out providing scientific basis during carry out hygienical evaluation.
The detection of microorganism in food generally use microscope observation and immunological detection, microscope observation by
Equipment and laboratory condition limitation, generally require technical professional in laboratory conditions using microscope to microorganism sample
This progress is observed and analyzed；Immune detection is a kind of immunological detection method based on antibody-antigen binding, to specific biological
Matter carries out the technology of qualitative or quantitative analysis.Antigen recognizes correspondence antibody by the epitope of antibody surface and combined, this identification
Also make immune detection that there is the specificity of height：Such as AIDS antigen can only be combined with aids antibody, without anti-with other
Precursor reactant.
The detection of existing antigen be typically by the operating personnel Jing Guo professional training laboratory environment using buying or
Homemade kit simultaneously combines such as fluorescence microscope instrument to complete.Because the necessary condition of antibody and antigen binding is antibody
Come close to or in contact with antigen, there is target antibody in the testing sample covered before dispatching from the factory in existing kit, testing sample leads to
Often need in laboratory by the pretreatment such as centrifugation, in addition, part detection is also needed to buy the antibody crossed of fluorescence labeling and
Into.Test experience result is typically to observe the change of color in kit, or is obtained with fluorescence microscopy fluorescent brightness
Take, achieved finally by manually result is recorded to preserve.Because testing staff needs professional training, testing result and detection people
The experience of member is related, and with certain subjectivity, the detection of antigen is caused indirectly larger systematic error.
To sum up, inventor has found, the problem of current immune detection is present has：Complex operation, the detection of many antigens is all needed
To be carried out in laboratory environment；Need to carry out special training to testing staff simultaneously, testing result has subjectivity；Except this it
Outside, detection cycle length, testing result time lag.
The content of the invention
It is an object of the invention to provide a kind of microorganism detection system and method, for solving existing microorganism detection skill
Need to carry out in laboratory conditions in art and the problem of detection efficiency is low.
In order to achieve the above object, the invention provides a kind of mobile test end, including：Test module, for detecting
Micro-biological samples are stated, to obtain the test result that micro-biological samples are reacted with microbial antibodies test solution；
Environment detection module, for obtaining mobile test end institute when micro-biological samples are reacted with microbial antibodies test solution
Locate the information of environment, the information of mobile test end local environment includes temperature, relative humidity and air pressure；
And the first signal transmitting and receiving module, for by the information of mobile test end local environment and test result hair
A data terminal is delivered to, and receives the microorganism concn analysis result that the data terminal is sent.
Optionally, prepared by the microbial antibodies test solution is specially：Filtration treatment, filtering are carried out to microbial antibodies test solution
Fall the impurity in the microbial antibodies test solution.
Optionally, the mobile test end is also including being used to record the locating module of test position information, believing for inputting
The input module of breath and the clock module for recording detection time, the locating module, the input module and the clock
Module is connected with the first signal transmitting and receiving module.
Present invention also offers a kind of data terminal, the data terminal includes：
Experiment sample database, for recording in multiple different temperatures values, multiple different rh values and multiple differences
Microbial antibodies test solution and micro-biological samples response data under the conditions of atmospheric pressure value；
Analysis module, for environmental information and test result and the experiment sample data for sending a mobile terminal
Data in storehouse are compared, to obtain the concentration of microorganism in the micro-biological samples；And secondary signal transmitting-receiving mould
Block, for receiving environmental information and test result that the mobile test end is sent, and the analysis result of analysis module is anti-
It is fed to the mobile test end.
Optionally, the preparation method of the experiment sample database includes：
Keep experiment rh value and experiment atmospheric pressure value constant, change experimental temperature value by the micro-biological samples and institute
The reaction of microbial antibodies test solution is stated, temperature experiment sample data are obtained；
Keep experimental temperature value and experiment atmospheric pressure value constant, change experiment rh value by the micro-biological samples and institute
The reaction of microbial antibodies test solution is stated, humidity experiment sample data are obtained；
Keep experimental temperature value and experiment rh value constant, change experiment atmospheric pressure value by the micro-biological samples and institute
The reaction of microbial antibodies test solution is stated, air pressure experimental data is obtained；
The temperature experimental data, the humidity experimental data and the air pressure fitting experimental data are obtained into the reaction
Experiment sample database.
Optionally, the micro-biological samples include the micro-biological samples of different microorganisms concentration, the experiment sample data
The preparation method in storehouse is specially：
Keep RH=50% and P=101.325kPa constant, in T=-50 ° of+0.5n1Under conditions of, by the microorganism
Sample reacts with the microbial antibodies test solution, obtains the temperature experimental data, n1For experiment number, n1For 0 to 100 it is whole
Number；
T=20 ° of holding and P=101.325kPa are constant, in RH=(n2- 1) under conditions of × 5%, by the microorganism
Sample reacts with the microbial antibodies test solution, obtains the humidity experimental data, n2For humidity experiment number, n2For 1 to 21
Integer；
T=20 ° of holding and RH=50% are constant, in P=(901.325+5n3) under conditions of kPa, by the microorganism sample
This reacts with the microbial antibodies test solution, obtains the air pressure experimental data, n3For air pressure experiment number, n3For 0 to 40 it is whole
Number；
T is the experimental temperature value, and RH is the experiment rh value, and P is the experiment atmospheric pressure value；
The temperature experimental data, the humidity experimental data and the air pressure fitting experimental data are obtained into the reaction
Experiment sample database.
Preferably, the data terminal also includes the memory module and use for being used to record the analysis module analysis result
In the display module for showing the analysis result, the memory module is connected with the analysis module signal.
Preferably, present invention also offers a kind of microorganism detection method, it includes：
S1：The micro-biological samples are detected at the mobile test end, are obtained the micro-biological samples and are resisted with the microorganism
The test result of body test solution, and by the mobile test end detect the ambient temperature value, the environmental wet angle value, institute
State ambient pressure value and the test result is sent to the data terminal；
S2：According to the ambient temperature value, the environmental wet angle value, the ambient pressure value from the experiment sample data
The micro-biological samples response data of various concentrations is obtained in storehouse；
S3：The response data is analyzed with the test result, micro- life in the micro-biological samples is obtained
The analysis result of thing concentration, and the analysis result is sent to the mobile test end.
Optionally, the formula of comparative analysis is in the S3：
Wherein, f0For the analysis result, f (T, RH, P) is the experiment sample database, P0For the ambient pressure
Value, T0For the ambient temperature value, RH0For the envionmental humidity, T1And T2For in the experiment sample database with T0Phase
Two adjacent experimental temperature values, laFor T1And T2Between threshold length, l1And l2Respectively T0Correspond to T1And T2Distance；RH1
And RH2For in the experiment sample database with RH0Two adjacent experiment rh values, lbFor RH1And RH2Between threshold value
Length, l3And l4Respectively RH0Correspond to RH1And RH2Distance；P1And P2For in the experiment sample database with P0Adjacent
Two experiment atmospheric pressure values, lcFor P1And P2Between threshold length, l5And l6Respectively P0Correspond to P1And P2Distance.
Compared with prior art, a kind of microorganism detection system and method that the present invention is provided, has the advantages that：
1st, without by entering in laboratory conditions in the outdoor extraction micro-biological samples of experiment and to the micro-biological samples
Row detection, increases portability by using mobile test end, while improving detection efficiency, slow down the time of testing result
It is delayed；
2nd, testing result is obtained by data analysis, more artificial subjective judgement accuracy is high, substitution manual record is achieved, drop
Low systematic error, prevents the cheating occurred during manual record, improves the accuracy of microorganism detection；
3rd, according to temperature, relative humidity and the pneumatic parameter of influence testing result, the broad experiment sample number of threshold value is formulated
According to storehouse, be well adapted for the detection to microorganism under the conditions of different geographical natural environment and climates, disclosure satisfy that remote districts and
The use requirement in the severe area of natural conditions；
4th, the personnel without specialty are operable, it is not necessary to carrying out professional technique training using crowd, reduced using behaviour
Make difficulty, be easy to promote the use of；
5th, mobile test end structure is simple, is easy to carry；
6th, mobile test end detection microorganism, by mobile communication transmission to data terminal, and then obtains different regions
Microorganism and its concentration data information, are delivered, prevention from suffering from the diseases and scientific research have certain meaning to disease treatment, medical resource
Justice.
Brief description of the drawings
Fig. 1 is mobile test end provided in an embodiment of the present invention and data terminal structure schematic diagram；
Fig. 2 is experiment sample database preparation method provided in an embodiment of the present invention；
Fig. 3 is microorganism detection method flow chart provided in an embodiment of the present invention；
Wherein, 1- test tubes, 21- temperature sensors, 22- relative humidity sensors, 23- baroceptors, 3- signal acquisitions
Module, 4- the first signal transmitting and receiving modules, 5- pumping signal modules, 6- secondary signal transceiver modules, 7- analysis modules, 8- experiment samples
Database, 9- mobile communication, 10- locating modules, 11- clock modules, 12- input modules, 13- memory modules.
Embodiment
The embodiment of the present invention is described in more detail below in conjunction with schematic diagram.According to description below and
Claims, advantages and features of the invention will become apparent from.It should be noted that, accompanying drawing uses very simplified form and equal
Using non-accurately ratio, only for the purpose of facilitating and clarifying the purpose of the embodiments of the invention.
Referring to Fig. 1, the embodiments of the invention provide a kind of mobile test end A, for detecting micro- life in micro-biological samples
Thing concentration, including：
Test module, for detecting the micro-biological samples, is reacted with obtaining micro-biological samples with microbial antibodies test solution
Test result；
Environment detection module, for obtaining mobile test end institute when micro-biological samples are reacted with microbial antibodies test solution
Locate the information of environment, the information of mobile test end local environment includes temperature, humidity and air pressure；
And the first signal transmitting and receiving module, for by the information of mobile test end local environment and test result hair
A data terminal is delivered to, and receives the microorganism concn analysis result that the data terminal is sent.
The test module includes the test tube 1 and signal acquisition module 3 for being used to deposit the microbial antibodies test solution, described
Microbial antibodies test solution is used for the microorganism concn for demarcating the micro-biological samples, and signal acquisition module 3 is used to gather institute
State the test result that micro-biological samples are reacted with the microbial antibodies test solution；
The environment detection module include be used for measure the mobile test end A ambient temperature values temperature sensor 21,
For measuring the humidity sensor 22 of the mobile test end A envionmental humidity values and for measuring the mobile test end A
The baroceptor 23 of ambient pressure value；
The test result that first signal transmitting and receiving module 4 gathers signal acquisition module 3, temperature sensor 21 are measured
The atmospheric pressure value that the envionmental humidity and baroceptor 23 that environment temperature, relative humidity sensor 22 are measured are measured is sent to number
According to end B, and receive the microorganism concn analysis result that data terminal B sends and come.
Inventor has found that existing microorganism detection technology, typically by gathering micro-biological samples, then carries out preservation,
Then the antibody sample collected is transported in laboratory, the micro-biological samples detected in laboratory environment,
Using this protocol step is various, testing result time lag, detection efficiency it is low, and need to carry out professional training to personnel
Required with meeting detection.Inventor has found that：Influence temperature of the environmental factor of microorganism detection when being mainly detection,
Envionmental humidity and air pressure, thus inventors herein propose non-laboratory condition (i.e. different temperature, envionmental humidity and
Under air pressure conditions) micro-biological samples are detected, it is then relatively wet by the way that itself and experiment in advance are obtained into different temperature, environment
Test experience result under degree and air pressure conditions is contrasted, and obtains the technical scheme of microorganism detection result.On realizing
Technical scheme is stated, a mobile test end A is designed first to the microbial antibodies test solution in micro-biological samples and test tube 1
Test result is acquired, while measuring temperature now by temperature sensor 21, being measured by relative humidity sensor 22
Relative humidity now and air pressure now is measured by baroceptor 23, then by test result, temperature, relative humidity and
Pressure information is sent to data terminal B by the first signal transmitting and receiving module, and the transmission is carried out in the way of mobile communication 9, institute
It is, for example, GSM, GPRS, 4G, 5G and/or wifi to state mobile communication 9.
Preferably, prepared by the microbial antibodies test solution is specially：The microbial antibodies test solution is filtered and carried
Pure processing, filters out the impurity in the microbial antibodies test solution, and then it is anti-to improve microorganism in the microbial antibodies test solution
The concentration of body, to reduce influence of the unrelated impurity to testing result in microbial antibodies test solution., can be by test tube in practical operation
The microbial antibodies test solution in 1 is prepared according to fixed standard, and the test tube 1 is used and is detachably connected installed in mobile test
Hold on A, when carrying out next micro-biological samples detection, change the outfit the new test tube 1 equipped with the microbial antibodies test solution, and then
Realize the detection to multiple micro-biological samples.
Preferably, mobile test end A is also including being used to record the locating module 10 of test position information, being detected for recording
The clock module 11 of time and the input module 12 for inputting information, it is the locating module 10, the clock module 11, described
Input module 12 is connected with the first signal transmitting and receiving module 4 and can realize data transfer.Carrying out microorganism detection
Meanwhile, positional information, temporal information during to detection record and are transferred to data terminal B by the first signal transmitting and receiving module 4.It is right
The time of microorganism and positional information are recorded, and are contributed to data terminal B to understand and are surveyed detection site and detection time, Jin Eryou
The area and tester for helping understand microbial profile need to detect the Annual distribution of microorganism, prevention and control, medical treatment money to disease
The input in source and scientific research have positive meaning.Input module 12 can be using input through keyboard, fingerprint input and/or hand
The mode of input is write, species, age, sex, nationality, healthy shape of the detection information marked as detected object are needed during to detection
Condition etc. is inputted and illustrated, helps to understand the further information of the microorganism, can be with hand if measurement object is people
The signature data and/or finger print information of input tester and detected person are write, objective, the fairness of testing result is improved.
Preferably, the mobile test end A also include being used for encouraging the microbial antibodies test solution in the test tube 1 with
The pumping signal module 5 of the micro-biological samples reaction, the pumping signal module 5 is connected with the test tube 1, the test tube
The microbial antibodies test solution in 1 mixes reaction of formation test solution, the work of the pumping signal module 5 with the micro-biological samples
Working frequency is 10Hz to 10MHz.When antibody in the microbial antibodies in the microbial antibodies test solution and the micro-biological samples
During anti-raw immune response, the change of voltage and/or electric current and signal phase can occur for the output signal of the pumping signal in test tube 1
Change, this change turns to the test result, signal acquisition module 3 is used to gather the voltage and/or electric current and letter occurred in test tube 1
The change of number phase, signal acquisition module 3 is connected with the signal of the first signal transmitting and receiving module 4, and the first signal transmitting and receiving module 4 is used for will
The test result is sent to the data terminal.
Traditional detection method is typically combined by the Brownian movement between antibody and antigen molecule, due to Blang's fortune
Dynamic speed causes testing result time lag more slowly, and the reaction test solution is persistently swashed using signal driving frequency
Encourage, increase the motion liveness of antibody and antigen molecule, it is possible to increase the reaction speed of the reaction test solution, and then reduce
Detection time, improves detection efficiency.
The embodiment of the present invention additionally provides a kind of data terminal B, for receiving, analyzing micro- life from mobile test end A
The signal that thing antibody test solution reacts with micro-biological samples, and analysis result is sent to mobile test end A, the data terminal bag
Include：
Experiment sample database 8, the experiment sample database 8 is used to record in multiple different temperatures values, multiple differences
Microbial antibodies test solution and micro-biological samples response data under the conditions of rh value and different atmospheric pressure values；
Analysis module 7, the analysis module 7 is used for the environmental information and test result for sending a mobile test end A
It is compared with the data in the experiment sample database 8, to obtain the concentration of microorganism in the micro-biological samples；
And secondary signal transceiver module 6, the secondary signal transceiver module 6 is for receiving the mobile test end A hairs
The environmental information and test result sent, and the analysis result of analysis module 6 is fed back into the mobile test end A.
Referring to Fig. 2, the preparation method of the experiment sample database is：
Keep experiment rh value and experiment atmospheric pressure value constant, change experimental temperature value by the micro-biological samples and institute
The reaction of microbial antibodies test solution is stated, temperature experiment sample data are obtained；
Keep experimental temperature value and experiment atmospheric pressure value constant, change experiment rh value by the micro-biological samples and institute
The reaction of microbial antibodies test solution is stated, humidity experiment sample data are obtained；
Keep experimental temperature value and experiment rh value constant, change experiment atmospheric pressure value by the micro-biological samples and institute
The reaction of microbial antibodies test solution is stated, air pressure experimental data is obtained；
The temperature experimental data, the humidity experimental data and the air pressure fitting experimental data are obtained into the reaction
Experiment sample database.
Preferably, the micro-biological samples include the micro-biological samples of different microorganisms concentration, the experiment sample data
The preparation in storehouse is specially：
Keep RH=50% and P=101.325kPa constant, in T=-50 ° of+0.5n1Under conditions of, by the microorganism
Sample reacts with the microbial antibodies test solution, obtains the temperature experimental data, n1For experiment number, n1For 0 to 100 it is whole
Number, temperature is different between each sample in temperature experimental data, and the measurement tolerance between each sample is 0.5 degree Celsius,
This measurement tolerance is taken to be based on temperature sensor resolution ratio, economic serviceability, experiment number and sample data volume for inventor
Consideration, preferred value is drawn by experimental verification, in a practical situation with the susceptibility of microorganism and microbial antibodies to temperature
Consider, more or less temperature experimental data samples, i.e. measurement tolerance between each sample can be gathered and taken the photograph for 1
Family name's degree ..., 0.4 degree Celsius, 0.3 degree Celsius, 0.2 degree Celsius, 0.1 degree Celsius ....It is note to take the purpose so tested
Record under certain relative humidity and air pressure conditions, control temperature change, observation temperature change to the micro-biological samples with it is described
The situation of microbial antibodies test solution reaction；
T=20 ° of holding and P=101.325kPa are constant, in RH=(n2- 1) under conditions of × 5%, by the microorganism
Sample reacts with the microbial antibodies test solution, obtains the humidity experimental data, n2For humidity experiment number, n2For 1 to 21
Integer, relative humidity is different between each sample in relative humidity experimental data, and the relative humidity between each sample is public
Difference is 1%, takes this relative humidity tolerance to be based on relative humidity sensor resolution ratio, economic serviceability, experiment for inventor
The consideration of number of times and sample data volume, preferred value is drawn by experimental verification, anti-with microorganism and microorganism in a practical situation
Body considers to the susceptibility of envionmental humidity, can gather more or less humidity experimental data samples, i.e., each
Relative humidity tolerance between individual sample is 10% ..., 4%, 3%, 2%, 1% ....Taking the purpose so tested is
Record controls relative humidity variations, observation relative humidity variations are to the micro-biological samples under certain temperature and air pressure conditions
Situation about being reacted with the microbial antibodies test solution；
T=20 ° of holding and RH=50% are constant, in P=(901.325+5n3) under conditions of kPa, by the microorganism sample
This reacts with the microbial antibodies test solution, obtains the air pressure experimental data, n3For air pressure experiment number, n3For 0 to 40 it is whole
Number, air pressure is different between each sample in air pressure experimental data, and the air pressure tolerance between each sample is 5kPa, takes this
Individual air pressure tolerance is consideration of the inventor based on baroceptor resolution ratio, economic serviceability, experiment number and sample data volume,
Preferred value is drawn by experimental verification, the susceptibility of air pressure is integrated with microorganism and microbial antibodies examined in a practical situation
Consider, can gather more or less air pressure experimental data samples, i.e. air pressure tolerance between each sample is
10kPa、……、4kPa、3kPa、2kPa、1kPa、…….Take the purpose so tested be recorded on certain relative humidity and
Under temperature conditionss, air pressure change is controlled, observation air pressure change is reacted the micro-biological samples and the microbial antibodies test solution
Situation；
T is the experimental temperature value, and RH is the experiment rh value, and P is the experiment atmospheric pressure value；By the temperature
Experimental data, the humidity experimental data and the air pressure fitting experimental data obtain the reaction experiment sample database.
Preferably, the data terminal B also include be used for record the memory module 13 of the analysis result of analysis module 7 with
And for showing the display module (not marked in figure) of the analysis result, the memory module 13 respectively with the analysis module
7 are connected, due to mobile test end A gather micro-biological samples from microbial antibodies test solution from different areas, Ran Houyi
Dynamic test lead A will collect information and be sent to data terminal B by telecommunication 9, therefore memory module 13 has different regions, no
Same geographical environment, different nationalities, Various Seasonal, the big data of all ages and classes micro-biological samples, this is provided for disease treatment, medical treatment
Source is delivered, prevention from suffering from the diseases and scientific research have certain meaning, while testing result is transferred to data terminal B, prevents artificial note
The cheating occurred during record.
Fig. 3 is refer to, present invention also offers a kind of microorganism detection method, it includes：
S1：The mobile test end A detects the micro-biological samples, obtains the micro-biological samples and resists with the microorganism
The test result of body test solution, and by the mobile test end A detect the ambient temperature value, the environmental wet angle value,
The ambient pressure value and the test result are sent to the data terminal B；
S2：According to the ambient temperature value, the environmental wet angle value, the ambient pressure value from the experiment sample data
The micro-biological samples response data of various concentrations is obtained in storehouse 8；
S3：The response data is analyzed with the test result, micro- life of the micro-biological samples is obtained
Thing concentration, and analysis result is sent to the mobile test end A.
Preferably, the formula of comparative analysis is in the S3：
Wherein, f0For the analysis result, f (T, RH, P) is the experiment sample database, P0For the ambient pressure
Value, T0For the ambient temperature value, RH0For the envionmental humidity, T1And T2For in the experiment sample database with T0Phase
Two adjacent experimental temperature values, laFor T1And T2Between threshold length, l1And l2Respectively T0Correspond to T1And T2Distance；RH1
And RH2For in the experiment sample database with RH0Two adjacent experiment rh values, lbFor RH1And RH2Between threshold value
Length, l3And l4Respectively RH0Correspond to RH1And RH2Distance；P1And P2For in the experiment sample database with P0Adjacent
Two experiment atmospheric pressure values, lcFor P1And P2Between threshold length, l5And l6Respectively P0Correspond to P1And P2Distance, using adding
Weight average algorithm, further increases the accuracy of testing result.
To sum up, in a kind of microorganism detection system and method provided in an embodiment of the present invention, a movement is designed first
Test lead is acquired to the test result of micro-biological samples and microbial antibodies test solution, while measurement temperature now, relative
Humidity and air pressure, then by test result, temperature, relative humidity and pressure information by being sent to data terminal, data terminal
The test experience result information of reception obtained from experiment in advance under different temperature, envionmental humidity and air pressure conditions is carried out
Contrast, obtains microorganism detection result, and testing result is sent into mobile test end, solves existing microorganism detection technology
In need to carry out in laboratory conditions, testing result time lag and the problem of low detection efficiency, reached and simplified detection
Method, increase detection device portability and the purpose for improving detection accuracy.
The preferred embodiments of the present invention are above are only, any restriction effect is not played to the present invention.Belonging to any
Those skilled in the art, in the range of technical scheme is not departed from, to the invention discloses technical scheme and
Technology contents make the variation such as any type of equivalent substitution or modification, belong to the content without departing from technical scheme, still
Belong within protection scope of the present invention.
Claims (10)
1. a kind of mobile test end, for detecting the microorganism concn in micro-biological samples, it is characterised in that the mobile test
End includes：
Test module, for detecting the micro-biological samples, to obtain the survey that micro-biological samples are reacted with microbial antibodies test solution
Test result；
Environment detection module, for obtaining ring residing for mobile test end when micro-biological samples are reacted with microbial antibodies test solution
The information in border, the information of mobile test end local environment includes temperature, relative humidity and air pressure；
And the first signal transmitting and receiving module, for by the information and test result of mobile test end local environment send to
One data terminal, and receive the microorganism concn analysis result that the data terminal is sent.
2. mobile test end as claimed in claim 1, it is characterised in that the microbial antibodies test solution is prepared and specifically included：
Filtration treatment and purification processes are carried out to the microbial antibodies test solution, to filter out in the microbial antibodies test solution
Impurity.
3. mobile test end as claimed in claim 1, it is characterised in that the mobile test end also includes：
Locating module for recording test position information；
Clock module for recording detection time；
And for inputting the input module of information.
4. a kind of data terminal, it is characterised in that the data terminal includes：
Experiment sample database, for recording in multiple different temperatures values, multiple different rh values and multiple different air pressure
Microbial antibodies test solution and micro-biological samples response data under the conditions of value；
Analysis module, for environmental information and test result and the experiment sample database for sending a mobile test end
In data be compared, to obtain the concentration of microorganism in the micro-biological samples；
And secondary signal transceiver module, for receiving environmental information and test result that the mobile test end is sent, and
The analysis result of analysis module is fed back into the mobile test end.
5. data terminal as claimed in claim 4, it is characterised in that the preparation method of the experiment sample database includes：
Keep experiment rh value and experiment atmospheric pressure value it is constant, change experimental temperature value by the micro-biological samples with it is described micro-
Biological antibody test solution reacts, and obtains temperature experiment sample data；
Keep experimental temperature value and experiment atmospheric pressure value it is constant, change experiment rh value by the micro-biological samples with it is described micro-
Biological antibody test solution reacts, and obtains humidity experiment sample data；
Keep experimental temperature value and experiment rh value it is constant, change experiment atmospheric pressure value by the micro-biological samples with it is described micro-
Biological antibody test solution reacts, and obtains air pressure experimental data；And
The temperature experimental data, the humidity experimental data and the air pressure fitting experimental data are obtained into the reaction experiment
Sample database.
6. data terminal as claimed in claim 5, it is characterised in that the micro-biological samples include different microorganisms concentration
Micro-biological samples, the preparation method of the experiment sample database is specially：
Keep RH=50% and P=101.325kPa constant, in T=-50 ° of+0.5n1Under conditions of, by the micro-biological samples
Reacted with the microbial antibodies test solution, obtain the temperature experimental data, n1For experiment number, n1For 0 to 100 integer；
T=20 ° of holding and P=101.325kPa are constant, in RH=(n2- 1) under conditions of × 5%, by the micro-biological samples
Reacted with the microbial antibodies test solution, obtain the humidity experimental data, n2For humidity experiment number, n2For 1 to 21 it is whole
Number；
T=20 ° of holding and RH=50% are constant, in P=(901.325+5n3) under conditions of kPa, by the micro-biological samples with
The microbial antibodies test solution reaction, obtains the air pressure experimental data, n3For air pressure experiment number, n3For 0 to 40 integer；
T is the experimental temperature value, and RH is the experiment rh value, and P is the experiment atmospheric pressure value；
The temperature experimental data, the humidity experimental data and the air pressure fitting experimental data are obtained into the reaction experiment
Sample database.
7. data terminal as claimed in claim 4, it is characterised in that the data terminal also includes：
For recording the memory module of the analysis module analysis result and display module for showing the analysis result,
The memory module is connected with the analysis module signal.
8. a kind of microorganism concn detecting system, it is characterised in that the microorganism detection system includes：
Mobile test end described in claim any one of 1-3；And any described data terminals of claim 4-7.
9. a kind of microorganism detection method, it is characterised in that the microorganism detection method includes：
S1：The micro-biological samples are detected at mobile test end, obtain the test that micro-biological samples are reacted with microbial antibodies test solution
As a result, and by the information and the test result of mobile test end local environment it is sent to a data terminal；
S2：The micro- of various concentrations is obtained from the experiment sample database according to the information of mobile test end local environment
Biological specimen response data；
S3：The micro-biological samples response data of the various concentrations and the test result are analyzed, obtain described
The analysis result of microorganism concn in micro-biological samples, and the analysis result is sent to the mobile test end.
10. a kind of microorganism detection method as claimed in claim 9, it is characterised in that the formula of comparative analysis in the S3
For：
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Wherein, f0For the analysis result, f (T, RH, P) is the experiment sample database, P0For the ambient pressure value, T0
For the ambient temperature value, RH0For the envionmental humidity, T1And T2For in the experiment sample database with T0Adjacent
Two experimental temperature values, laFor T1And T2Between threshold length, l1And l2Respectively T0Correspond to T1And T2Distance；RH1With
RH2For in the experiment sample database with RH0Two adjacent experiment rh values, lbFor RH1And RH2Between threshold value it is long
Degree, l3And l4Respectively RH0Correspond to RH1And RH2Distance；P1And P2For in the experiment sample database with P0Adjacent two
Individual experiment atmospheric pressure value, lcFor P1And P2Between threshold length, l5And l6Respectively P0Correspond to P1And P2Distance.
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