CN203759010U - Quantum dot labeled test strip card - Google Patents
Quantum dot labeled test strip card Download PDFInfo
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- CN203759010U CN203759010U CN201320710132.4U CN201320710132U CN203759010U CN 203759010 U CN203759010 U CN 203759010U CN 201320710132 U CN201320710132 U CN 201320710132U CN 203759010 U CN203759010 U CN 203759010U
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Abstract
The utility model relates to the field of in vitro diagnosis and in particular relates to a quantum dot labeled test strip card. The quantum dot labeled test strip card comprises a card case (3) and a quantum dot labeled test strip (2), wherein an opening is formed at one end of the card case (3) to form a test strip slot (13); the test strip (2) is a separate plug-and-use unit of the card case (3); a storage medium (14) in which the information such as standard curves of detected samples is stored is installed on the card case (3). When samples are detected, one end of a sample sucking pad (4) of the test strip (2) inserted in the card case (3) extends out of the opening end of the card case (3) in an interference manner so as to be soaked for sucking the samples, then an instrument with the signal detection function is used for collecting the characteristic signals of detection bands (6) and a quality control band (7) of the test strip (2), and standard curves or coefficient parameters of detected substances, which are simultaneously read by the used instrument from the storage medium (14), are also available, so that single-component or multicomponent concentrations of the samples are obtained through calculation. The test strip card has the characteristics of simplicity and quickness, high sensitivity, objective results, flexibility in use and the like when being used for detecting samples.
Description
Technical field
The utility model belongs to in-vitro diagnosis field, be specifically related to a kind of self with the information for detection such as checking matter typical curve or coefficient parameter, in conjunction with thering is the instrument energy fast quantification sample one-component of signal testing function or the quantum dot-labeled examination bar card of polycomponent concentration.
Background technology
Colloidal gold immunochromatographimethod technology (Gold-Immunochromatography Assay, GICA) various aspects of immune detection have now been widely used in, it take miillpore filter as carrier, utilize the capillarity of miillpore filter, make the fluid sample dripping in film bar one end slowly to the miillpore filter other end, ooze and move, if have specific antigen or antibody in liquid, they can with Immuno gold in conjunction with after the corresponding antibodies on being coated on miillpore filter or antigen are combined and are shown Immuno gold color (redness) again.This technology is simple and quick, a few minutes the result that just can detect by an unaided eye.Weak point is: (1) can only qualitative detection sample, and it is quantitative that difficulty can realize sample.(2) difficulty can accomplish that sample polycomponent detects simultaneously, and detection efficiency is low.(3) for some antigen or the extremely low sample of antibody content, collaurum very slight color is difficult to the naked eye judged result, and detection sensitivity is low.
Realize sample polycomponent Quantitative detection, be the long-term targets of pursuing of people always.The nano-quantum point of developed recently (quantum dots, QDs), has unique good optical property: fluorescence radiation efficiency is high, exciting line wide ranges, energy " elementary excitation, polynary transmitting ", spectral line of emission narrow range and symmetry, photobleaching speed is slow, fluorescence lifetime is long, and particle diameter and biomolecule are close, energy multifunction after finishing, the characteristic wavelength fluorescence spectrum that the quantum dot potpourri of different-grain diameter and kind produces is not overlapping, is very suitable for sample multicomponent analysis.Sweeny etc. by three kinds of primary antibodies respectively with quantum dot (the λ em=705nm of three kinds of different emission, 605nm and 655nm) by specific binding formation antibody-quantum dot compound of biotin-avidin, when having realized in tonsillotome 3 kinds of different antibodies by fluorescence imaging, analyze, prove that employing multicolor quantum dot can locate multiple biomarker in same sample simultaneously.Quantum dot-labeled 4 kinds of antibody of 4 kinds of different emission of the use such as Nie have been realized the Reed-Sternberg to the significant cell HRS(Hodgkin ' of pathology in Hodgkin lymphoma s and) imaging detect, improve detection sensitivity and specificity, be conducive to the clinical Hodgkin lymphoma of diagnosing rapidly and accurately.Kobayashi etc. and lymphatic vessel stream and the left and right thoracic cavity of Chan philosophy with the near-infrared quantum dots imaging analysis mouse of two kinds of different colours, proved the multiple imaging dissection of near-infrared quantum dots.Utilize the fluorescent characteristic of quantum dot can also realize the qualitative and quantitative analysis to multiple pathogens, toxin, antigen, antibody, enzyme, small-molecule substance and ion etc. except carrying out imaging analysis.Zahavy etc. prepare fluorescence probe QD585-IgG α B.anthracis and QD655-IgG α Y.pestis, and the simultaneous quantitative of having realized two kinds of pathogenic bacteria in conjunction with flow cytometer detects, and detectability can reach 10
3cfu/mL.When having realized enterotoxin B that staphylococcus produces and TNT and cholera toxin, ricin (WA), shiga toxin and staphylococcal entotoxin four kinds of toxin with color quantum point, Goldman seminar detects.In addition, in microfluid protein-chip, with quantum dot, make fluorescence labeling, utilize its polyfluoro chromatism fast detecting tumor markers carcinomebryonic antigen (CEA) and alpha-fetoprotein (AFP) simultaneously, detectability is low to moderate 250fmol/L, than detect high 4 orders of magnitude with organic fluorescent dye.Giorgio etc. form fluorescence probe with the quantum dot of different emission respectively by VEGF-A (VEGF A) and angiogenin (angiopoietin), by antigen and antibody specific association reaction, cause the gathering of quantum dot, thereby with the quantitative detected characteristics fluorescence signal of flow cytometer, obtain the concentration of corresponding antigens, detection is limited to 1pmol/L, when having realized plurality of antigens, detects.In the last few years, many researchers utilized the method for quantum-dot coding to carry out multicomponent analysis.This technology is mainly to encode by the rich of quantum dot fluorescence color and fluorescence intensity, can measure more component simultaneously, can realize the Synchronization Analysis of the magnanimity information to containing in the researchs such as protein analysis, DNA analysis and drug screening.In theory,
nplant intensity
mplanting color can produce
n m -a kind of code, utilizes decoding technique to realize right
n m the analyzing and testing of-a kind of object.According to calculating, only need combine from the quantum dot nano particle of 6 kinds of different luminous intensities by 5～6 kinds of colors, just can obtain 10000～40000 discernible nano particle coding microballs.If combined from the quantum dot nano particle of 10 kinds of different luminous intensities, can obtain 1,000,000 discernible nano particle coding microballs, these microballoons could carry out coded markings to 1,000,000 different DNA or protein in theory, thereby realize, 1,000,000 different DNA in sample or protein are detected simultaneously! Nie etc. wrap up the quantum dot of three kinds of different colours of red, green, blue into polystyrene microsphere and encode with the ratio of 1:1:1,1:2:1,2:1:1, when having realized three kinds of target dnas by the different qualities of gained fluorescence spectrum, detect.Adopt similar techniques, Gu and Sha etc. encode the quantum dot of two kinds of different emission to have realized respectively the quantitative detection of multiple not homotactic single stranded DNA and the high flux screening somatotype of multiple oligonucleotides to the hydrogel of DNA responsive type and microballoons respectively with three kinds of intensity.When having realized three kinds of haemocyanins (chicken ovalbumin OVA, bovine serum albumin(BSA) BSA, human serum albumins HAS) and four kinds of immunoglobulin Gs (human IgG, rabbit igg, mouse IgG, sheep IgG), this technology of utilizing Wilson and Cooper philosophy detects.
China's application number patent 200410010736.3, with colloidal gold labeled monoclonal antibody, be combined with object, by immune response coated quantum dot-labeled antibody on film, be combined, form and detect band, with the naked eye to detecting band, carrying out on the basis of preliminary qualitative or half-quantitative detection, the fluorescent quenching effect of recycling collaurum to fluorescent nano particles, quantitatively detects test strip by detecting the cancellation degree of fluorescence signal.This patent will be with two kinds of particle marks, and method is simple not; Adopt the cancellation degree of fluorescence signal quantitatively to detect, disturbing factor is many, and detection sensitivity is low.
China's application number patent 200610024086.7 has disclosed a kind of detection method of quantum dot mark fast immune chromatographic test paper bar.Though this patent can realize polycomponent and detect simultaneously, what ask for protection is a kind of method, and the protection of not filing a request is based on quantum dot mark fast immune chromatographic test paper bar product.And, the method Shortcomings: (1) realizes the detection of sample polycomponent need to arrange many corresponding detection lines on analyzing film simultaneously, and every detection line is only for detection of a kind of index in sample, and detection efficiency is low.If tester wants synchronously to detect in sample a large amount of components, flux component, magnanimity component even, a slight examination bar can not be realized detection target by so numerous corresponding detection lines are set.(2) thus adopt ultra violet lamp ELISA test strip band and quality control band with observe its photoluminescence line and have or not or power judgement sample in whether contain object, truly say, this is still a kind of qualitative detection, or can only reach at the most the detectable range of certain checking matter is measured, can not fundamentally realize the accurate concentration detection of component arbitrarily in sample, sensitivity is still low.
Adopting the quantitative checking matter concentration of checking matter standard items typical curve, is the main method of the quantitative sample concentration of detection field.Message area is the storage medium of development recently, as RFID label (claiming again RFID tag), IC chip, magnetic code, bar code etc., for Information Access identification, the fields such as computing machine, communication, electronics, business, communications and transportation control and management have now been widely used in, wherein, RFID label especially, volume is little, storage information capacity is large, identification need not manual intervention, the desirable means of Information Access identification especially, but there be limited evidence currently of has people to use it for biomedical detection.
The utility model is for shortcomings and deficiencies of the prior art, a kind of quanta dot mark test bar card that storage medium 14 is installed on it is provided, and this storage medium 14 stores the detection information such as the typical curve used with the quantitative checking matter concentration of batch quanta dot mark test bar 2 or coefficient parameter.Sample completes on bar 2 after the reaction of examination bar in examination, by the instrument collection examination bar card cartridge 3 interior examination bars 2 with signal testing function, is detected with 6 and the characteristic signal of quality control band 7 read the checking matter typical curve that comes or coefficient parameter and calculate and obtain sample single component or polycomponent concentration from storage medium 14 in conjunction with this instrument simultaneously.The utility model for quantitative sample concentration particularly sample polycomponent concentration have easy quick, highly sensitive, result is objective, use the features such as flexible.
Utility model content
Technical solutions of the utility model are as follows:
Quantum dot-labeled examination bar card described in the utility model, comprises cartridge 3 and quanta dot mark test bar 2.Described quanta dot mark test bar 2 comprises that overlap joint is fixed on suction sample pad 4, quantum dot-labeled pad 5, analyzing film 8, the adsorptive pads 9 on end liner 1 in turn.Analyzing film 8 has to detect is with 6 and quality control band 7.One end of cartridge 3 forms examination bar slot mouth 13 together with the upper box face opening of this cartridge end, to facilitate quanta dot mark test bar 2 to insert now in cartridge 3.What examination bar 2 was cartridge 3 is inserting structure from formula.During sample detection, it is inhaled the openend that the 4 one end interference of sample pad stretch out cartridge 3 and draws sample with dipping to be inserted into examination bar in cartridge 32.The upper box face of cartridge 3 is having detection window 11 corresponding to examination bar analyzing film 8 positions.A storage is installed in cartridge 3 has examination bar to detect the storage medium 14 of information for sample.
The quantum dot-labeled pad 5 of described quanta dot mark test bar 2 is coated with single quantum dot-labeled a certain target checking matter and detects relevant single specific antigen (or antibody) molecule, or: each target checking matter that is coated with different single quantum dot correspondence markings detects the potpourri of relevant specific antigen (or antibody) molecule.Quantum dot-labeled pad 5 coated described single quantum dots comprise ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag
2s, CdS/PbS, CdS/Cd (0H)
2, CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, MgSe, MgTe, CaS, CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS:Mn, ZnS:Mn, CdS:Cu, ZnS:Cu, CdS:Tb, the combination of any one in ZnS:Tb or any several nano particles, and be core by above-mentioned any one quantum dot, silicon dioxide is the core-shell type nano-complex particle of shell.
The detection of described quanta dot mark test bar 2 is with 6 can be one, also can be many.Detection is coated with a certain target checking matter with 6 and detects relevant specific antibody (or antigen) molecule, or is coated with the potpourri that each target checking matter detects relevant specific antibody (or antigen) molecule.
The quality control band 7 of described quanta dot mark test bar 2 is coated with Quality Control thing.Described Quality Control thing comprises that two is anti-.
Described storage medium 14 includes but not limited to RFID label, IC chip, magnetic code or bar code etc.The examination bar that storage medium 14 stores detects by information and comprises that the checking matter typical curve used with the quantitative sample concentration of batch quanta dot mark test bar 2 or coefficient parameter, examination bar quality control band optical density reference monitor value, examination bar lot number, examination bar imitate phase, storage medium password, clinical indices reference value, Shi Tiao production firm information etc., and can read in the information such as measurand identity information, tester's information, sample title, sample number into spectrum, detection date, testing result.
The checking matter typical curve that described storage medium 14 stores has various ways available, and it includes but not limited to following curve: checking matter standard items series concentration and OD
detect band/ OD
quality control bandbetween corresponding relation curve; Or, checking matter standard items series concentration and OD
detect band/ (OD
detect band+ OD
quality control band) between corresponding relation curve.Wherein, OD
detect bandfor the detection band optical density value that checking matter standard items series concentration records, OD
quality control bandthe quality control band optical density value recording for checking matter standard items series concentration.
After the adsorptive pads 9 of quanta dot mark test bar 2 described in the utility model, can also be overlapped with an examination bar reaction end indicating label 10.Correspondingly, these examination bar reaction end indicating label 10 corresponding positions of its cartridge 3 have view window 12.Described examination bar reaction end indicating label 10 comprises that color change interval is the pH test paper of 5-9.
The quantum dot-labeled pad 5 of quanta dot mark test bar 2 described in the utility model is glass fibre membrane; The analyzing film 8 of described quanta dot mark test bar 2 is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films; The end liner 1 of described quanta dot mark test bar 2 is polyester or plastic plate; Described cartridge 3 is that polyester, plastics, rigid paper material or other materials are made.
Quanta dot mark test bar described in the utility model 2 is the disposable articles for use that make, the storage medium 14 in described cartridge 3 and cartridge 3 for batch quanta dot mark test bar 2 supporting make articles for use.
When quanta dot mark test bar card described in the utility model is used for sample detection, in the current insertion cartridge 3 of its quanta dot mark test bar 2, be inserted into the interference external part dipping imbitition sample of the examination bar 2 in cartridge 3.Fluid sample completes on bar 2 after the reaction of examination bar in examination, gathers examination bar 2 detect with 6 and the characteristic signal of quality control band 7 read the checking matter typical curve that comes or coefficient parameter and calculate and obtain sample single component or polycomponent concentration from examination bar card storage medium 14 in conjunction with this instrument simultaneously by the instrument (as reading bar instrument, fluorescence detector etc.) with signal testing function.
The sample to be checked of quanta dot mark test bar card described in the utility model can be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, clinical sample comprises the sample of infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease etc., and non-clinical sample comprises the sample of food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection etc.
Compared with prior art, the utlity model has following beneficial effect:
(1) described examination bar card can be realized sample polycomponent in conjunction with the instrument (as reading bar instrument, fluorescence detector etc.) with signal testing function and quantitatively detect simultaneously and rapidly (comprising qualitative detection).The quantum dot of different-grain diameter, kind and structure can produce different characteristic wavelength and continue fluorescence, the characteristic wavelength fluorescence peak spectrum that quantum dot potpourri produces is not overlapping, the utility model adopts different quantum dots mark checking matter corresponding antigens/antibody response molecule respectively, its potpourri is coated on the examination bar 2 of described examination bar card and reacts with testing sample, by mensuration, try bar 2 characteristic fluorescence signals, thereby can fast quantification sample polycomponent concentration.
(2) detect the sample time short, a few minutes just can obtain result.The quantitative sample concentration of the utility model does not need operator to make checking matter typical curve again, and the typical curve of checking matter or coefficient parameter etc. detect with on the standby storage storage medium 14 of information.During sample detection, operator only need insert the instrument with signal testing function by the examination bar card that is applied with sample after it completes the reaction of examination bar, by this instrument, gathering its examination bar 2 detects with 6 and the characteristic fluorescence signal of quality control band 7, in conjunction with this instrument, from storage medium 14, read corresponding checking matter typical curve or the coefficient parameter come, a few minutes just can quantitatively obtain sample detection result simultaneously.
(3) can carry out dynamic monitoring to examination bar chromatography course of reaction.The utility model detects the Reinhoit Zahl (OD of band and quality control band according to examination bar
detect band/ OD
quality control bandor OD
detect band/ (OD
detect band+ OD
quality control band)) or coefficient parameter and in conjunction with the checking matter typical curve of storage medium 14 storage by instrument (as reading bar instrument, fluorescence detector etc.) the Analysis deterrmination checking matter concentration with signal testing function.The quality control band optical density reference monitor value (OD of checking matter standard items while also storing standard curve making on storage medium 14
quality control band ').If the failure of examination bar detection reaction, the quality control band optical density value (OD that testing process obtains
quality control band) can with the quality control band optical density reference monitor value (OD of storage medium 14 storage
quality control band ') there is very big statistical errors, prompting examination bar reacts unsuccessfully.Typical curve on storage medium 14 and OD
quality control band 'with in examination bar card, try bar 2 and match, the checking matter concentration checkout discrepancy that may cause because of batch mass discrepancy to overcome the built-in examination bar 2 of examination bar card.The tail end of examination bar 2 described in the utility model also can be provided with examination bar reaction end indicating label 10, this label be used to refer to used instrument its reactant on 2 before examination bar 2 detects whether fully ooze move to examination bar detect be with 6 and quality control band 7 to be applicable to use instrument can to examination bar detect be with 6 and quality control band 7 carry out autoscan, thereby reach the success or failure details that dynamic monitoring tries bar chromatography course of reaction.
(4) detect quality and adopt multiple indexes synchro control, make sample quantitatively more accurate: 1. try, on bar 2, quality control band 7 is set, its optical density intensity works the contrast effect that detects.2. storage medium 14 is stored corresponding checking matter typical curve or coefficient parameters, for quantitative corresponding checking matter concentration.3. storage medium 14 storages try bar quality control band optical density reference monitor value (OD
quality control band '), for monitoring the reaction of sample detection pilot scale bar, whether set up, point out testing result whether effective.4. try to arrange on bar 2 examination bar reaction end indicating label, the siphon of prompting examination bar reactant on examination bar oozes whether move chromatography abundant, and whether reaction reaches home.
(5) the examination bar 2 in described examination bar card cartridge 3 is disposable use examination bar; Described examination bar card cartridge 3 and the make articles for use of the storage medium in cartridge 3 14 for matching with batch examination bar 2 that are used for inserting now disposable test strips 2.During sample detection, for saving tester's expense, with a batch examination bar 2, can take to continue to share with batch examination bar card cartridge 3 that examination bar 2 matches to implement sample detection.Examination bar divalent lattice are cheap, and ordinary consumer can be born.
(6) amount of samples is few, and a few microlitre to tens microlitres just can meet detection needs.
0025](7) in examination bar card, reagent and material do not relate to active inactivation, can preserve for a long time in room temperature.
Accompanying drawing explanation
Figure l: the plan structure figure of quanta dot mark test bar card preferred version one described in the utility model (show and wherein insert detection line of examination strip adoption)
Fig. 2: the side-looking structural drawing of inserting examination bar that quanta dot mark test bar card preferred version one described in the utility model matches (showing detection line of examination strip adoption)
Fig. 3: the plan structure figure of quanta dot mark test bar card preferred version one described in the utility model (show and wherein insert many detection lines of examination strip adoption)
Fig. 4: the side-looking structural drawing of inserting examination bar that quanta dot mark test bar card preferred version one described in the utility model matches (showing many detection lines of examination strip adoption)
Fig. 5: the plan structure figure of another preferred version of quanta dot mark test bar card described in the utility model (show and wherein insert detection line of examination strip adoption)
Fig. 6: the side-looking structural drawing of inserting examination bar that another preferred version of quanta dot mark test bar card described in the utility model matches (showing detection line of examination strip adoption)
Fig. 7: the plan structure figure of another preferred version of quanta dot mark test bar card described in the utility model (show and wherein insert many detection lines of examination strip adoption)
Fig. 8: the side-looking structural drawing of inserting examination bar that another preferred version of quanta dot mark test bar card described in the utility model matches (showing many detection lines of examination strip adoption)
Sequence number is expressed as follows:
1, end liner, 2, examination bar, 3, cartridge, 4, inhale sample pad, 5, quantum dot-labeled pad, 6, detect band, 7, quality control band, 8, analyzing film, 9, adsorptive pads, 10, examination bar reaction end indicating label, 11, detection window, 12, view window, 13, examination bar slot mouth, 14, storage medium
Embodiment
Embodiment and accompanying drawing thereof are only in order to further illustrate the utility model below, and those skilled in the art should not limit protection domain of the present utility model with this.
embodiment mono-
Fig. 1-4 explanation embodiment mono-.Embodiment mono-is one of quanta dot mark test bar card preferred version described in the utility model.
In Fig. 1-4, the described quanta dot mark test bar card of described preferred version comprises cartridge 3 and quanta dot mark test bar 2.Described quanta dot mark test bar 2 comprises that overlap joint is fixed on suction sample pad 4, quantum dot-labeled pad 5, analyzing film 8, the adsorptive pads 9 on end liner 1 in turn.Analyzing film 8 has to detect is with 6 and quality control band 7.One end of cartridge 3 forms examination bar slot mouth 13 together with the upper box face opening of this cartridge end, to facilitate quanta dot mark test bar 2 to insert now in cartridge 3.What examination bar 2 was cartridge 3 is inserting structure from formula.During sample detection, it is inhaled the openend that the 4 one end interference of sample pad stretch out cartridge 3 and draws sample with dipping to be inserted into examination bar in cartridge 32.The upper box face of cartridge 3 is having detection window 11 corresponding to examination bar analyzing film 8 positions.A storage is installed in cartridge 3 has examination bar to detect the storage medium 14 of information for sample.
The quantum dot-labeled pad 5 of described quanta dot mark test bar 2 is coated with single quantum dot-labeled a certain target checking matter and detects relevant single specific antigen (or antibody) molecule; Or: each target checking matter that is coated with different single quantum dot correspondence markings detects the potpourri of relevant specific antigen (or antibody) molecule.The following particle of the optional use of described single quantum dot of the quantum dot-labeled pad 5 of examination bar 2, comprises ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag
2s, CdS/PbS, CdS/Cd (0H)
2, CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, MgSe, MgTe, CaS, CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS:Mn, ZnS:Mn, CdS:Cu, ZnS:Cu, CdS:Tb, the combination of any one in ZnS:Tb or any several nano particles, and be core by above-mentioned any one quantum dot, silicon dioxide is the core-shell type nano-complex particle of shell.
The detection of described quanta dot mark test bar 2 is with 6 can be one (shown in Fig. 1 and Fig. 2), can be also many (shown in Fig. 3 and Fig. 4).Detection is coated with a certain target checking matter with 6 and detects relevant specific antibody (or antigen) molecule; Or: be coated with the potpourri that each target checking matter detects relevant specific antibody (or antigen) molecule.
The quality control band 7 of described quanta dot mark test bar 2 is coated with Quality Control thing.Described Quality Control thing comprises that two is anti-.
Described storage medium 14 includes but not limited to RFID label, IC chip, magnetic code or bar code etc.The examination bar of its storage detects by information and comprises that the checking matter typical curve used with the quantitative sample concentration of batch quanta dot mark test bar 2 or coefficient parameter, examination bar quality control band optical density reference monitor value, examination bar lot number, examination bar imitate phase, storage medium password, clinical indices reference value, Shi Tiao production firm information etc., and can read in the information such as measurand identity information, tester's information, sample title, sample number into spectrum, detection date, testing result.
The checking matter typical curve that described storage medium 14 stores has various ways available, and it includes but not limited to following curve: checking matter standard items series concentration and OD
detect band/ OD
quality control bandbetween corresponding relation curve; Or, checking matter standard items series concentration and OD
detect band/ (OD
detect band+ OD
quality control band) between corresponding relation curve.Wherein, OD
detect bandfor the detection band optical density value that checking matter standard items series concentration records, OD
quality control bandthe quality control band optical density value recording for checking matter standard items series concentration.
The quantum dot-labeled pad 5 of described quanta dot mark test bar 2 is glass fibre membrane; The analyzing film 8 of described quanta dot mark test bar 2 is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films; The end liner 1 of described quanta dot mark test bar 2 is polyester or plastic plate; Described cartridge 3 is that polyester, plastics, rigid paper material or other materials are made.
Described quanta dot mark test bar 2 is the disposable articles for use that make, the storage medium 14 in described cartridge 3 and cartridge 3 for batch quanta dot mark test bar 2 supporting make articles for use.
When described quanta dot mark test bar card is used for sample detection, in the current insertion cartridge 3 of its quanta dot mark test bar 2, be inserted into the interference external part dipping imbitition sample of the examination bar 2 in cartridge 3.Fluid sample completes on bar 2 after the reaction of examination bar in examination, gathers examination bar 2 detect with 6 and the characteristic signal of quality control band 7 read the checking matter typical curve that comes or coefficient parameter and calculate and obtain sample single component or polycomponent concentration from examination bar card storage medium 14 in conjunction with this instrument simultaneously by the instrument (as reading bar instrument, fluorescence detector etc.) with signal testing function.
The sample to be checked of described quanta dot mark test bar card can be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, clinical sample comprises the sample of infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease etc., and non-clinical sample comprises the sample of food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection etc.
embodiment bis-
Fig. 5-8 explanation embodiment bis-.Embodiment bis-is another preferred version of quanta dot mark test bar card described in the utility model.
In Fig. 5-8, the described quanta dot mark test bar card of described preferred version comprises cartridge 3 and quanta dot mark test bar 2.Described quanta dot mark test bar 2 comprises that overlap joint is fixed on suction sample pad 4, quantum dot-labeled pad 5, analyzing film 8, adsorptive pads 9 and the examination bar reaction end indicating label 10 on end liner 1 in turn.Analyzing film 8 has to detect is with 6 and quality control band 7.One end of cartridge 3 forms examination bar slot mouth 13 together with the upper box face opening of this cartridge end, to facilitate quanta dot mark test bar 2 to insert now in cartridge 3.What examination bar 2 was cartridge 3 is inserting structure from formula.During sample detection, it is inhaled the openend that the 4 one end interference of sample pad stretch out cartridge 3 and draws sample with dipping to be inserted into examination bar in cartridge 32.The upper box face of cartridge 3 is having detection window 11 corresponding to examination bar analyzing film 8 positions, the upper box face of cartridge 3 is having view window 12 corresponding to examination bar reaction end indicating label 10 positions, and a storage is installed in cartridge 3 has examination bar to detect the storage medium 14 of information for sample.
The quantum dot-labeled pad 5 of described quanta dot mark test bar 2 is coated with single quantum dot-labeled a certain target checking matter and detects relevant single specific antigen (or antibody) molecule; Or: each target checking matter that is coated with different single quantum dot correspondence markings detects the potpourri of relevant specific antigen (or antibody) molecule.The following particle of the optional use of described single quantum dot of the quantum dot-labeled pad 5 of examination bar, comprises ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag
2s, CdS/PbS, CdS/Cd (0H)
2, CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, MgSe, MgTe, CaS, CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS:Mn, ZnS:Mn, CdS:Cu, ZnS:Cu, CdS:Tb, the combination of any one in ZnS:Tb or any several nano particles, and be core by above-mentioned any one quantum dot, silicon dioxide is the core-shell type nano-complex particle of shell.
The detection of described quanta dot mark test bar 2 is with 6 can be one (shown in Fig. 5 and Fig. 6), can be also many (shown in Fig. 7 and Fig. 8).Detection is coated with a certain target checking matter with 6 and detects relevant specific antibody (or antigen) molecule; Or: be coated with the potpourri that each target checking matter detects relevant specific antibody (or antigen) molecule.
The quality control band 7 of described quanta dot mark test bar 2 is coated with Quality Control thing.Described Quality Control thing comprises that two is anti-.
Described examination bar reaction end indicating label 10 comprises that color change interval is the pH test paper of 5-9.During sample detection, examination bar reaction end indicating label 10 on examination bar can develop the color according to the pH condition of examination bar reaction, whether with prompting examination bar reactant, fully ooze to move past to detect and be with 6 and quality control band 7, whether the reaction of prompting examination bar is abundant, whether its testing result is effective.
Described storage medium 14 includes but not limited to RFID label, IC chip, magnetic code or bar code etc.The examination bar of its storage detects by information and comprises that the checking matter typical curve used with the quantitative sample concentration of batch quanta dot mark test bar 2 or coefficient parameter, examination bar quality control band optical density reference monitor value, examination bar lot number, examination bar imitate phase, storage medium password, clinical indices reference value, Shi Tiao production firm information etc., and can read in the information such as measurand identity information, tester's information, sample title, sample number into spectrum, detection date, testing result.
The checking matter typical curve that described storage medium 14 stores has various ways available, and it includes but not limited to following curve: checking matter standard items series concentration and OD
detect band/ OD
quality control bandbetween corresponding relation curve; Or, checking matter standard items series concentration and OD
detect band/ (OD
detect band+ OD
quality control band) between corresponding relation curve.Wherein, OD
detect bandfor the detection band optical density value that checking matter standard items series concentration records, OD
quality control bandthe quality control band optical density value recording for checking matter standard items series concentration.
The quantum dot-labeled pad 5 of described quanta dot mark test bar 2 is glass fibre membrane; The analyzing film 8 of described quanta dot mark test bar 2 is nitrocellulose filter, nylon membrane or cellulose nitrate/cellulose acetate hybrid films; The end liner 1 of described quanta dot mark test bar 2 is polyester or plastic plate; Described cartridge 3 is that polyester, plastics, rigid paper material or other materials are made.
Described quanta dot mark test bar 2 is the disposable articles for use that make.Storage medium in described cartridge 3 and cartridge 3 14 for batch quanta dot mark test bar 2 supporting make articles for use.
When described quanta dot mark test bar card is used for sample detection, in the current insertion cartridge 3 of its quanta dot mark test bar 2, be inserted into the interference external part dipping imbitition sample of the examination bar 2 in cartridge 3.Examination bar reaction end indicating label 10 colour developing indication samples complete after the reaction of examination bar on examination bar 2, gather examination bar 2 detect with 6 and the characteristic signal of quality control band 7 read the checking matter typical curve that comes or coefficient parameter and calculate and obtain sample single component or polycomponent concentration from examination bar card storage medium 14 in conjunction with this instrument simultaneously by the instrument (as reading bar instrument, fluorescence detector etc.) with signal testing function.
The sample to be checked of described quanta dot mark test bar card can be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, clinical sample comprises the sample of infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease etc., and non-clinical sample comprises the sample of food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection etc.
It is to be noted, quantum dot-labeled examination bar card described in the utility model can also have other improvement, as it is upper outside except being arranged on the cartridge 3 of examination bar card with the storage medium 14 of information to store the detections such as checking matter typical curve, also can be directly installed on quanta dot mark test bar 2; Based on the utility model same principle, be inserted into quanta dot mark test bar in cartridge 3 and also can expand and replace with quantum dot-labeled biochip (comprising antigen-antibody chip, protein chip, nucleic acid chip, micro-fluidic chip) etc.Therefore, every to any other technical scheme that is equal to replacement or equivalent transformation formation of quanta dot mark test bar card employing described in the utility model, all drop in the protection domain of the utility model claim.
Claims (7)
1. a quantum dot-labeled examination bar card, comprise cartridge (3) and quanta dot mark test bar (2), quanta dot mark test bar (2) comprises that overlap joint is fixed on the suction sample pad (4) on end liner (1), quantum dot-labeled pad (5), analyzing film (8), adsorptive pads (9) in turn, analyzing film (8) has the band of detection (6) and quality control band (7), detecting band (6) is one or more, the upper box face of cartridge (3) is having detection window (11) corresponding to examination bar analyzing film (8) position, it is characterized in that:
One end of described cartridge (3) forms examination bar slot mouth (13) together with the upper box face opening of this cartridge end, what examination bar (2) was cartridge (3) is inserting structure from formula, and it is inhaled sample pad (4) one end interference and stretches out cartridge (3) openend and draw sample with dipping to be inserted into examination bar (2) in cartridge (3);
A storage is installed in described cartridge (3) has examination bar to detect the storage medium (14) of information for sample.
2. quantum dot-labeled examination bar card according to claim 1, is characterized in that: described storage medium (14) comprises RFID label, IC chip, magnetic code or bar code.
3. quantum dot-labeled examination bar card according to claim 1, it is characterized in that: the quantum dot-labeled pad (5) of described quanta dot mark test bar (2) is glass fibre membrane, and the analyzing film (8) of described quanta dot mark test bar (2) is nitrocellulose filter or nylon membrane; The end liner (1) of described quanta dot mark test bar (2) is polyester or plastic plate; Described cartridge (3) is that polyester, plastics or rigid paper material are made.
4. a quantum dot-labeled examination bar card as claimed in claim 1, is characterized in that: the adsorptive pads (9) of wherein said quanta dot mark test bar (2) is also overlapped with an examination bar reaction end indicating label (10) afterwards; This examination bar reaction end indicating label (10) corresponding position of its cartridge (3) has view window (12).
5. quantum dot-labeled examination bar card according to claim 4, is characterized in that, described examination bar reaction end indicating label (10) comprises that color change interval is the pH test paper of 5-9.
6. quantum dot-labeled examination bar card according to claim 4, is characterized in that: described storage medium (14) comprises RFID label, IC chip, magnetic code or bar code.
7. quantum dot-labeled examination bar card according to claim 4, it is characterized in that: the quantum dot-labeled pad (5) of described quanta dot mark test bar (2) is glass fibre membrane, and the analyzing film (8) of described quanta dot mark test bar (2) is nitrocellulose filter or nylon membrane; The end liner (1) of described quanta dot mark test bar (2) is polyester or plastic plate; Described cartridge (3) is that polyester, plastics or rigid paper material are made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320710132.4U CN203759010U (en) | 2013-11-12 | 2013-11-12 | Quantum dot labeled test strip card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320710132.4U CN203759010U (en) | 2013-11-12 | 2013-11-12 | Quantum dot labeled test strip card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203759010U true CN203759010U (en) | 2014-08-06 |
Family
ID=51254266
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320710132.4U Expired - Lifetime CN203759010U (en) | 2013-11-12 | 2013-11-12 | Quantum dot labeled test strip card |
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| Country | Link |
|---|---|
| CN (1) | CN203759010U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015070750A1 (en) * | 2013-11-16 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card |
| WO2015070746A1 (en) * | 2013-11-14 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card marked by quantum dots |
| WO2015070704A1 (en) * | 2013-11-13 | 2015-05-21 | 成都领御生物技术有限公司 | Test bar card |
| WO2015070700A1 (en) * | 2013-11-12 | 2015-05-21 | 成都领御生物技术有限公司 | Quantum dot-labeled test strip card |
-
2013
- 2013-11-12 CN CN201320710132.4U patent/CN203759010U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015070700A1 (en) * | 2013-11-12 | 2015-05-21 | 成都领御生物技术有限公司 | Quantum dot-labeled test strip card |
| WO2015070704A1 (en) * | 2013-11-13 | 2015-05-21 | 成都领御生物技术有限公司 | Test bar card |
| WO2015070746A1 (en) * | 2013-11-14 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card marked by quantum dots |
| WO2015070750A1 (en) * | 2013-11-16 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |