CN203759011U - Quantum dot labeled test strip card - Google Patents
Quantum dot labeled test strip card Download PDFInfo
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- CN203759011U CN203759011U CN201320716016.3U CN201320716016U CN203759011U CN 203759011 U CN203759011 U CN 203759011U CN 201320716016 U CN201320716016 U CN 201320716016U CN 203759011 U CN203759011 U CN 203759011U
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Abstract
The utility model relates to the field of in vitro diagnosis and in particular relates to a quantum dot labeled test strip card. The quantum dot labeled test strip card comprises a card case (3) and a quantum dot labeled test strip (2), wherein a storage medium (11) in which the information for detection, such as standard curves and the like of detected samples is stored is installed on the card case. When samples are detected, the test strip card is characterized in that an instrument with the signal detection function is used for collecting the characteristic signals of detection bands (6) of the test strip (2) of the test strip card and standard curves or coefficient parameters of detected objects, which are simultaneously read by the used instrument from the storage medium (11), are combined to obtain single-component or multicomponent concentrations of the samples through calculation. The test strip card has the characteristics of simplicity and quickness, high sensitivity, objective results, flexibility in use and the like when being used for detecting samples.
Description
Technical field
The utility model belongs to in-vitro diagnosis field, be specifically related to a kind of self with the information for detection such as checking matter typical curve or coefficient parameter, in conjunction with thering is the instrument energy fast quantification sample one-component of signal testing function or the quantum dot-labeled examination bar card of polycomponent concentration.
Background technology
Colloidal gold immunochromatographimethod technology (Gold-Immunochromatography Assay, GICA) various aspects of immune detection have now been widely used in, it is taking miillpore filter as carrier, utilize the capillarity of miillpore filter, make to drip and slowly ooze and move to the miillpore filter other end at the fluid sample of film bar one end, if have specific antigen or antibody in liquid, they can with Immuno gold in conjunction with after the corresponding antibodies on being coated on miillpore filter or antigen are combined and are shown Immuno gold color (redness) again.This technology is simple and quick, a few minutes the result that just can detect by an unaided eye.Weak point is: (1) can only qualitative detection sample, and it is quantitative that difficulty can realize sample.(2) difficulty can accomplish that sample polycomponent detects simultaneously, and detection efficiency is low.(3) for some antigen or the extremely low sample of antibody content, collaurum very slight color is difficult to the naked eye judged result, and detection sensitivity is low.
Realize sample polycomponent Quantitative detection, be the long-term targets of pursuing of people always.The nano-quantum point (quantum dots, QDs) of developed recently, has unique good optical property: fluorescence radiation efficiency is high, exciting line wide ranges, energy " elementary excitation, polynary transmitting ", spectral line of emission narrow range and symmetry, photobleaching speed is slow, fluorescence lifetime is long, and particle diameter and biomolecule are close, energy multifunction after finishing, the characteristic wavelength fluorescence spectrum that the quantum dot potpourri of different-grain diameter and kind produces is not overlapping, is very suitable for sample multicomponent analysis.Sweeny etc. by three kinds of primary antibodies respectively with quantum dot (the λ em=705nm of three kinds of different emission, 605nm and 655nm) form antibody-quantum dot compound by the specific binding of biotin-avidin, when having realized in tonsillotome 3 kinds of different antibodies by fluorescence imaging, analyze, prove that employing multicolor quantum dot can locate multiple biomarker in same sample simultaneously.Quantum dot-labeled 4 kinds of antibody of 4 kinds of different emission of the use such as Nie have been realized the Reed-Sternberg to the significant cell HRS(Hodgkin ' of pathology in Hodgkin lymphoma s and) imaging detect, improve detection sensitivity and specificity, be conducive to the clinical Hodgkin lymphoma of diagnosing rapidly and accurately.Kobayashi etc. and lymphatic vessel stream and the left and right thoracic cavity of Chan philosophy with the near-infrared quantum dots imaging analysis mouse of two kinds of different colours, proved the multiple imaging dissection of near-infrared quantum dots.Utilize the fluorescent characteristic of quantum dot can also realize the qualitative and quantitative analysis to multiple pathogens, toxin, antigen, antibody, enzyme, small-molecule substance and ion etc. except carrying out imaging analysis.Zahavy etc. prepare fluorescence probe QD585-IgG α B.anthracis and QD655-IgG α Y.pestis, and the simultaneous quantitative of having realized two kinds of pathogenic bacteria in conjunction with flow cytometer detects, and detectability can reach 10
3cfu/mL.When having realized enterotoxin B that staphylococcus produces and TNT and cholera toxin, ricin (WA), shiga toxin and staphylococcal entotoxin four kinds of toxin with color quantum point, Goldman seminar detects.In addition, in microfluid protein-chip, make fluorescence labeling with quantum dot, utilize its polyfluoro chromatism fast detecting tumor markers carcinomebryonic antigen (CEA) and alpha-fetoprotein (AFP) simultaneously, detectability is low to moderate 250fmol/L, than detect high 4 orders of magnitude with organic fluorescent dye.Giorgio etc. form fluorescence probe with the quantum dot of different emission respectively by VEGF-A (VEGF A) and angiogenin (angiopoietin), cause the gathering of quantum dot by antigen and antibody specific association reaction, thereby quantitatively detect characteristic fluorescence signal and obtain the concentration of corresponding antigens with flow cytometer, detection is limited to 1pmol/L, when having realized plurality of antigens, detects.In the last few years, many researchers utilized the method for quantum-dot coding to carry out multicomponent analysis.This technology is mainly to encode by the rich of quantum dot fluorescence color and fluorescence intensity, can measure more component simultaneously, can realize the Synchronization Analysis of the magnanimity information to containing in the researchs such as protein analysis, DNA analysis and drug screening.In theory,
nplant intensity
mplanting color can produce
n m -a kind of code, utilizes decoding technique to realize right
n m the analyzing and testing of-a kind of object.According to calculating, only need combine from the quantum dot nano particle of 6 kinds of different luminous intensities by 5～6 kinds of colors, just can obtain 10000～40000 discernible nano particle coding microballs.If combined from the quantum dot nano particle of 10 kinds of different luminous intensities, can obtain 1,000,000 discernible nano particle coding microballs, these microballoons could carry out coded markings to 1,000,000 different DNA or protein in theory, thereby realize, 1,000,000 different DNA in sample or protein are detected simultaneously! Nie etc. wrap up the quantum dot of three kinds of different colours of red, green, blue into polystyrene microsphere and encode with the ratio of 1:1:1,1:2:1,2:1:1, when having realized three kinds of target dnas, detect by the different qualities of gained fluorescence spectrum.Adopt similar techniques, with three kinds of intensity, the hydrogel to DNA responsive type and microballoon are encoded and have been realized respectively the quantitative detection of multiple not homotactic single stranded DNA and the high flux screening somatotype of multiple oligonucleotides respectively by the quantum dot of two kinds of different emission for Gu and Sha etc.When having realized three kinds of haemocyanins (chicken ovalbumin OVA, bovine serum albumin(BSA) BSA, human serum albumins HAS) and four kinds of immunoglobulin Gs (human IgG, rabbit igg, mouse IgG, sheep IgG), this technology of utilizing Wilson and Cooper philosophy detects.
China's application number patent 200410010736.3, be combined with object with colloidal gold labeled monoclonal antibody, be combined by immune response coated quantum dot-labeled antibody on film, form and detect band, with the naked eye carrying out detecting band on the basis of preliminary qualitative or half-quantitative detection, the fluorescent quenching effect of recycling collaurum to fluorescent nano particles, quantitatively detects test strip by the cancellation degree that detects fluorescence signal.Two kinds of particle marks for this patent, method is simple not; Adopt the cancellation degree of fluorescence signal quantitatively to detect, disturbing factor is many, and detection sensitivity is low.
China's application number patent 200610024086.7 has disclosed a kind of detection method of quantum dot mark fast immune chromatographic test paper bar.Though this patent can realize polycomponent and detect simultaneously, what request was protected is a kind of method, and the protection of not filing a request is based on quantum dot mark fast immune chromatographic test paper bar product.And, the method Shortcomings: (1) realizes the detection of sample polycomponent need to arrange many corresponding detection lines on analyzing film simultaneously, and every detection line is only for detection of a kind of index in sample, and detection efficiency is low.If tester wants synchronously to detect in sample a large amount of components, flux component, magnanimity component even, a slight examination bar can not be realized detection target by so numerous corresponding detection lines are set.(2) thus adopt ultra violet lamp test strips with observe its photoluminescence line and have or not or power judgement sample in whether contain object, truly say, this is still a kind of qualitative detection, or can only reach at the most the detectable range of certain checking matter is measured, can not fundamentally realize the accurate concentration detection of component arbitrarily in sample, sensitivity is still low.
Adopting the quantitative checking matter concentration of checking matter standard items typical curve, is the main method of the quantitative sample concentration of detection field.Message area is the storage medium of development recently, as RFID label (claiming again RFID tag), IC chip, magnetic code, bar code etc., the fields such as computing machine, communication, electronics, business, communications and transportation control and management are now widely used in for Information Access identification, wherein, especially RFID label, volume is little, storage information capacity is large, identification need not manual intervention, the desirable means of Information Access identification especially, but there be limited evidence currently of has people to use it for biomedical detection.
The utility model is for shortcomings and deficiencies of the prior art, a kind of quanta dot mark test bar card that storage medium 11 is installed on it is provided, and this storage medium 11 stores the detection information such as typical curve or coefficient parameter of using with the quantitative checking matter concentration of batch quanta dot mark test bar 2.Sample completes on bar 2 after the reaction of examination bar in examination, tries bar 2 and detects with 6 characteristic signal and in conjunction with this instrument and read the checking matter typical curve that comes or coefficient parameter simultaneously and calculate and obtain sample single component or polycomponent concentration from storage medium 11 by having in the instrument collection examination bar card of signal testing function.The utility model for quantitative sample concentration particularly sample polycomponent concentration have easy quick, highly sensitive, result is objective, use the features such as flexible.
Utility model content
Technical solutions of the utility model are as follows:
Quantum dot-labeled examination bar card described in the utility model, comprises the quanta dot mark test bar 2 of cartridge 3 and Qi Nei.Described quanta dot mark test bar 2 comprises that overlap joint is fixed on sample pad 4, quantum dot-labeled pad 5, analyzing film 7, the adsorptive pads 8 on end liner 1 in turn.Analyzing film 7 has to detect is with 6.The upper box face of cartridge 3 is having well 9 corresponding to examination bar sample pad 4 positions, and the upper box face of cartridge 3 is having detection window 10 corresponding to examination bar analyzing film 7 positions.A storage is installed in described cartridge 3 has examination bar to detect the storage medium 11 of sample information.
The quantum dot-labeled pad 5 of described quanta dot mark test bar 2 is coated with single quantum dot-labeled a certain target checking matter and detects relevant single specific antigen (or antibody) molecule, or: the each target checking matter that is coated with different single quantum dot correspondence markings detects the potpourri of relevant specific antigen (or antibody) molecule.Quantum dot-labeled pad 5 coated described single quantum dots comprise ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag
2s, CdS/PbS, CdS/Cd (0H)
2, CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, MgSe, MgTe, CaS, CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS:Mn, ZnS:Mn, CdS:Cu, ZnS:Cu, CdS:Tb, the combination of any one in ZnS:Tb or any several nano particles, and be core by above-mentioned any one quantum dot, silicon dioxide is the core-shell type nano-complex particle of shell.
The detection of described quanta dot mark test bar 2 is with 6 can be one, also can be many.Detection is coated with a certain target checking matter with 6 and detects relevant specific antibody (or antigen) molecule, or is coated with each target checking matter and detects the potpourri of relevant specific antibody (or antigen) molecule.
Described storage medium 11 includes but not limited to RFID label, IC chip, magnetic code or bar code etc.Examination bar that storage medium 11 stores detects by information and comprises that the checking matter typical curve used with the quantitative sample concentration of batch quanta dot mark test bar 2 or coefficient parameter, examination bar lot number, examination bar imitate phase, storage medium password, clinical indices reference value, Shi Tiao production firm information etc., and can read in the information such as measurand identity information, tester's information, sample title, sample number into spectrum, detection date, testing result.
The checking matter typical curve that described storage medium 11 stores has various ways available, and it includes but not limited to checking matter standard items series concentration and OD
detect bandbetween corresponding relation curve.Described OD
detect bandbe defined as the detection band optical density value that checking matter standard items series concentration records.
After the adsorptive pads 8 of quanta dot mark test bar 2 described in the utility model, can also be overlapped with an examination bar reaction end indicating label 12.Correspondingly, these examination bar reaction end indicating label 12 corresponding positions of its cartridge 3 have view window 13.Described examination bar reaction end indicating label 12 comprises that color change interval is the pH test paper of 5-9.
The quantum dot-labeled pad 5 of quanta dot mark test bar 2 described in the utility model is glass fibre membrane; The analyzing film 7 of described quanta dot mark test bar 2 is nitrocellulose filter; The end liner 1 of described quanta dot mark test bar 2 is polyester or plastic plate; Described cartridge 3 is that polyester, plastics, rigid paper material or other materials are made.
When sample detection, the examination bar that is applied with sample has been stuck in after the reaction of examination bar chromatography, gather its examination bar 2 by the instrument with signal testing function and detect the characteristic signal with 6, and read the corresponding checking matter typical curve that comes or coefficient parameter simultaneously and calculate and obtain sample single component or polycomponent concentration from storage medium 11 in conjunction with this instrument.
The sample to be checked of quanta dot mark test bar card described in the utility model can be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, the sample of clinical sample including infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease etc., the sample of non-clinical sample including food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection etc.
Compared with prior art, the utlity model has following beneficial effect:
(1) described examination bar card can be realized sample polycomponent in conjunction with the instrument (as reading bar instrument, fluorescence detector etc.) with signal testing function and quantitatively detect simultaneously and rapidly (comprising qualitative detection).The quantum dot of different-grain diameter, kind and structure can produce different characteristic wavelength and continue fluorescence, the characteristic wavelength fluorescence peak spectrum that quantum dot potpourri produces is not overlapping, the utility model adopts different quantum dots mark checking matter corresponding antigens/antibody response molecule respectively, its potpourri is coated on the examination bar 2 of described examination bar card and reacts with testing sample, try bar 2 characteristic fluorescence signals by mensuration, thereby can fast quantification sample polycomponent concentration.
(2) detect the sample time short, a few minutes just can obtain result.The quantitative sample concentration of the utility model does not need operator to make checking matter typical curve again, and the typical curve of checking matter or coefficient parameter etc. detect with on the standby storage storage medium 11 of information.When sample detection, operator only need insert the instrument with signal testing function by the examination bar card that is applied with sample after it completes the reaction of examination bar, gather by this instrument the characteristic fluorescence signal that its examination bar 2 detects with 6, read corresponding checking matter typical curve or the coefficient parameter come from storage medium 11 in conjunction with this instrument, a few minutes just can quantitatively obtain sample detection result simultaneously.
(3) amount of samples is few, and a few microlitre to tens microlitres just can meet detection needs.
(4) in examination bar card, reagent and material do not relate to active inactivation, can preserve for a long time in room temperature.
Brief description of the drawings
Figure l: the plan structure figure (showing detection line of examination strip adoption in it) of quanta dot mark test bar card preferred version one described in the utility model
Fig. 2: the side-looking structural drawing of examination bar in the cartridge of quanta dot mark test bar card preferred version one described in the utility model (showing detection line of examination strip adoption)
Fig. 3: the plan structure figure (showing many detection lines of examination strip adoption in it) of quanta dot mark test bar card preferred version one described in the utility model
Fig. 4: the side-looking structural drawing of examination bar in the cartridge of quanta dot mark test bar card preferred version one described in the utility model (showing many detection lines of examination strip adoption)
Fig. 5: the plan structure figure (showing detection line of examination strip adoption in it) of another preferred version of quanta dot mark test bar card described in the utility model
Fig. 6: the side-looking structural drawing of examination bar in the cartridge of another preferred version of quanta dot mark test bar card described in the utility model (showing detection line of examination strip adoption)
Fig. 7: the plan structure figure (showing many detection lines of examination strip adoption in it) of another preferred version of quanta dot mark test bar card described in the utility model
Fig. 8: the side-looking structural drawing of examination bar in the cartridge of another preferred version of quanta dot mark test bar card described in the utility model (showing many detection lines of examination strip adoption)
Sequence number is expressed as follows:
1, end liner, 2, examination bar, 3, cartridge, 4, sample pad, 5, quantum dot-labeled pad, 6, detect band, 7, analyzing film, 8, adsorptive pads, 9, well, 10, detection window, 11, storage medium, 12, examination bar reaction end indicating label, 13, view window
Embodiment
Embodiment and accompanying drawing thereof are only in order to further illustrate the utility model below, and those skilled in the art should not limit protection domain of the present utility model with this.
embodiment mono-
Fig. 1-4 explanation embodiment mono-.Embodiment mono-is one of quanta dot mark test bar card preferred version described in the utility model.
In Fig. 1-4, the described quanta dot mark test bar card of described preferred version comprises cartridge 3 and quanta dot mark test bar 2.Described quanta dot mark test bar 2 comprises that overlap joint is fixed on sample pad 4, quantum dot-labeled pad 5, analyzing film 7, the adsorptive pads 8 on end liner 1 in turn.Analyzing film 7 has to detect is with 6.The upper box face of cartridge 3 is having well 9 corresponding to examination bar sample pad 4 positions, and the upper box face of cartridge 3 is having detection window 10 corresponding to examination bar analyzing film 7 positions.A storage is installed in cartridge 3 has examination bar to detect the storage medium 11 of sample information.
The quantum dot-labeled pad 5 of described quanta dot mark test bar 2 is coated with single quantum dot-labeled a certain target checking matter and detects relevant single specific antigen (or antibody) molecule, or: the each target checking matter that is coated with different single quantum dot correspondence markings detects the potpourri of relevant specific antigen (or antibody) molecule.The following particle of the optional use of described single quantum dot of the quantum dot-labeled pad 5 of examination bar 2, comprises ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag
2s, CdS/PbS, CdS/Cd (0H)
2, CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, MgSe, MgTe, CaS, CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS:Mn, ZnS:Mn, CdS:Cu, ZnS:Cu, CdS:Tb, the combination of any one in ZnS:Tb or any several nano particles, and be core by above-mentioned any one quantum dot, silicon dioxide is the core-shell type nano-complex particle of shell.
The detection of described quanta dot mark test bar 2 is with 6 can be one (shown in Fig. 1 and Fig. 2), can be also many (shown in Fig. 3 and Fig. 4).Detection is coated with a certain target checking matter with 6 and detects relevant specific antibody (or antigen) molecule, or: the potpourri that is coated with each target checking matter and detects relevant specific antibody (or antigen) molecule.
Described storage medium 11 includes but not limited to RFID label, IC chip, magnetic code or bar code etc.The examination bar of its storage detects by information and comprises that the checking matter typical curve used with the quantitative sample concentration of batch quanta dot mark test bar 2 or coefficient parameter, examination bar lot number, examination bar imitate phase, storage medium password, clinical indices reference value, Shi Tiao production firm information etc., and can read in the information such as measurand identity information, tester's information, sample title, sample number into spectrum, detection date, testing result.
The checking matter typical curve that described storage medium 11 stores has various ways available, and it includes but not limited to checking matter standard items series concentration and OD
detect bandbetween corresponding relation curve.Described OD
detect bandbe defined as the detection band optical density value that checking matter standard items series concentration records.
The quantum dot-labeled pad 5 of described quanta dot mark test bar 2 is glass fibre membrane; The analyzing film 7 of described quanta dot mark test bar 2 is nitrocellulose filter; The end liner 1 of described quanta dot mark test bar 2 is polyester or plastic plate; Described cartridge 3 is that polyester, plastics, rigid paper material or other materials are made.
When sample detection, described quanta dot mark test bar card gathers its examination bar 2 by the instrument with signal testing function and detects the characteristic signal with 6, and reads the checking matter typical curve that comes or coefficient parameter simultaneously and calculate and obtain sample single component or polycomponent concentration from storage medium 11 in conjunction with this instrument.
The sample to be checked of described quanta dot mark test bar card can be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, the sample of clinical sample including infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease etc., the sample of non-clinical sample including food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection etc.
embodiment bis-
Fig. 5-8 explanation embodiment bis-.Embodiment bis-is another preferred version of quanta dot mark test bar card described in the utility model.
In Fig. 5-8, the described quanta dot mark test bar card of described preferred version comprises cartridge 3 and quanta dot mark test bar 2.Described quanta dot mark test bar 2 comprises that overlap joint is fixed on sample pad 4, quantum dot-labeled pad 5, analyzing film 7, adsorptive pads 8, the examination bar reaction end indicating label 12 on end liner 1 in turn.Analyzing film 7 has to detect is with 6.The upper box face of cartridge 3 is having well 9 corresponding to examination bar sample pad 4 positions, the upper box face face of cartridge 3 is having detection window 10 corresponding to examination bar analyzing film 7 positions, the upper box face of cartridge 3 is having view window 13 corresponding to examination bar reaction end indicating label 12 positions, and a storage is installed in cartridge 3 has examination bar to detect the storage medium 11 of sample information.
The quantum dot-labeled pad 5 of described quanta dot mark test bar 2 is coated with single quantum dot-labeled a certain target checking matter and detects relevant single specific antigen (or antibody) molecule, or: the each target checking matter that is coated with different single quantum dot correspondence markings detects the potpourri of relevant specific antigen (or antibody) molecule.The following particle of the optional use of described single quantum dot of the quantum dot-labeled pad 5 of examination bar, comprises ZnS, CdS, HgS, ZnSe, CdSe, HgSe, CdTe, ZnTe, ZnO, PbSe, HgTe, CaAs, InP, InAs, InCaAs, CdS/ZnS, CdS/Ag
2s, CdS/PbS, CdS/Cd (0H)
2, CdS/HgS, CdS/HgS/CdS, ZnS/CdS, ZnS/CdS/ZnS, ZnS/HgS/ZnS/CdS, CdSe/CdS, CdSe/ZnS, CdSe/ZnSe, CdSe/CuSe, CdSe/HgTe, CdSe/HgSe, CdSe/HgSe/CdSe, CdTe/HgS, CdTe/HgTe, InAs/InP, InAs/CdSe, InAs/ZnSe, MgS, MgSe, MgTe, CaS, CaSe, CaTe, SrS, SrSe, SeTe, BaS, BaSe, BaTe, CdS:Mn, ZnS:Mn, CdS:Cu, ZnS:Cu, CdS:Tb, the combination of any one in ZnS:Tb or any several nano particles, and be core by above-mentioned any one quantum dot, silicon dioxide is the core-shell type nano-complex particle of shell.
The detection of described quanta dot mark test bar 2 is with 6 can be one (shown in Fig. 5 and Fig. 6), can be also many (shown in Fig. 7 and Fig. 8).Detection is coated with a certain target checking matter with 6 and detects relevant specific antibody (or antigen) molecule, or: the potpourri that is coated with each target checking matter and detects relevant specific antibody (or antigen) molecule.
Described examination bar reaction end indicating label 12 includes but not limited to that color change interval is the pH test paper of 5-9.When sample detection, the examination bar reaction end indicating label 12 of examination on bar can develop the color according to the pH condition of examination bar reaction, is with 6 to point out examination bar reactant whether fully to ooze to move past to detect, and whether the reaction of prompting examination bar is abundant, and whether its testing result is effective.
Described storage medium 11 includes but not limited to RFID label, IC chip, magnetic code or bar code etc.The examination bar of its storage detects by information and comprises that the checking matter typical curve used with the quantitative sample concentration of batch quanta dot mark test bar 2 or coefficient parameter, examination bar lot number, examination bar imitate phase, storage medium password, clinical indices reference value, Shi Tiao production firm information etc., and can read in the information such as measurand identity information, tester's information, sample title, sample number into spectrum, detection date, testing result.
The checking matter typical curve that described storage medium 11 stores has various ways available, and it includes but not limited to checking matter standard items series concentration and OD
detect bandbetween corresponding relation curve.Described OD
detect bandbe defined as the detection band optical density value that checking matter standard items series concentration records.
The quantum dot-labeled pad 5 of described quanta dot mark test bar 2 is glass fibre membrane; The analyzing film 7 of described quanta dot mark test bar 2 is nitrocellulose filter; The end liner 1 of described quanta dot mark test bar 2 is polyester or plastic plate; Described cartridge 3 is that polyester, plastics, rigid paper material or other materials are made.
When described quanta dot mark test bar card is used for sample detection, fluid sample is applied in the well 9 of examination bar card, examination bar reaction end indicating label 12 colour developing instruction examination bars 2 until examination bar 2 in it complete after reaction, the characteristic signal being detected with 6 by the instrument collection examination bar 2 with signal testing function, and read the checking matter typical curve that comes or coefficient parameter simultaneously and calculate and obtain sample single component or polycomponent concentration from storage medium 11 in conjunction with this instrument.
The sample to be checked of described quanta dot mark test bar card can be from clinical or non-clinical blood, body fluid, urine, saliva, genital secretion or other liquid samples or thick sample, wherein, the sample of clinical sample including infectious disease, hormone, cardiovascular disease, tumour, cancer, diabetes, autoimmunity disease etc., the sample of non-clinical sample including food inspection, environment pollution detection, persticide residue detection, biological contamination detection, Biopreparate detection, veterinary science detection, illicit drugs inspection etc.
It is to be noted, quantum dot-labeled examination bar card described in the utility model can also have other improvement, as upper outside except being arranged on the cartridge 3 of examination bar card in the storage medium 11 that stores the detection information such as checking matter typical curve, also can be directly installed on quanta dot mark test bar 2; Based on the utility model same principle, the quanta dot mark test bar in cartridge 3 also can be expanded and replace with quantum dot-labeled biochip (comprising antigen-antibody chip, protein chip, nucleic acid chip, micro-fluidic chip) etc.Therefore, every to any other technical scheme that is equal to replacement or equivalent transformation formation of quanta dot mark test bar card employing described in the utility model, all drop in the protection domain of the utility model claim.
Claims (7)
1. a quantum dot-labeled examination bar card, comprise cartridge (3) and the quanta dot mark test bar (2) in it, quanta dot mark test bar (2) comprises that overlap joint is fixed on the sample pad (4) on end liner (1) in turn, quantum dot-labeled pad (5), analyzing film (7), adsorptive pads (8), analyzing film (7) has the band of detection (6), detecting band (6) is one or more, the upper box face of cartridge (3) is having well (9) corresponding to examination bar sample pad (4) position, the upper box face of cartridge (3) is having detection window (10) corresponding to examination bar analyzing film (7) position, it is characterized in that: a storage is installed in described cartridge (3) has examination bar to detect the storage medium (11) of sample information.
2. quantum dot-labeled examination bar card according to claim 1, is characterized in that: described storage medium (11) comprises RFID label, IC chip, magnetic code or bar code.
3. quantum dot-labeled examination bar card according to claim 1, it is characterized in that: the quantum dot-labeled pad (5) of described quanta dot mark test bar (2) is glass fibre membrane, the analyzing film (7) of described quanta dot mark test bar (2) is nitrocellulose filter, the end liner (1) of described quanta dot mark test bar (2) is polyester or plastic plate, and described cartridge (3) is that polyester, plastics or rigid paper material are made.
4. a quantum dot-labeled examination bar card as claimed in claim 1, is characterized in that: the adsorptive pads (8) of wherein said quanta dot mark test bar (2) is also overlapped with an examination bar reaction end indicating label (12) afterwards; This examination bar reaction end indicating label (12) corresponding position of its cartridge (3) has view window (13).
5. quantum dot-labeled examination bar card according to claim 4, is characterized in that, described examination bar reaction end indicating label (12) comprises that color change interval is the pH test paper of 5-9.
6. quantum dot-labeled examination bar card according to claim 4, is characterized in that: described storage medium (11) comprises RFID label, IC chip, magnetic code or bar code.
7. quantum dot-labeled examination bar card according to claim 4, it is characterized in that: the quantum dot-labeled pad (5) of described quanta dot mark test bar (2) is glass fibre membrane, the analyzing film (7) of described quanta dot mark test bar (2) is nitrocellulose filter, the end liner (1) of described quanta dot mark test bar (2) is polyester or plastic plate, and described cartridge (3) is that polyester, plastics or rigid paper material are made.
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| CN201320716016.3U CN203759011U (en) | 2013-11-14 | 2013-11-14 | Quantum dot labeled test strip card |
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| CN201320716016.3U CN203759011U (en) | 2013-11-14 | 2013-11-14 | Quantum dot labeled test strip card |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015070704A1 (en) * | 2013-11-13 | 2015-05-21 | 成都领御生物技术有限公司 | Test bar card |
| WO2015070700A1 (en) * | 2013-11-12 | 2015-05-21 | 成都领御生物技术有限公司 | Quantum dot-labeled test strip card |
| WO2015070749A1 (en) * | 2013-11-16 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card |
| WO2015070699A1 (en) * | 2013-11-12 | 2015-05-21 | 成都领御生物技术有限公司 | Quantum dot-labeled test strip card |
| CN107287291A (en) * | 2017-06-02 | 2017-10-24 | 华南师范大学 | A kind of double labelling nucleic acid detection method interacted based on g C3N4 and CdTe/CdS quantum dots |
-
2013
- 2013-11-14 CN CN201320716016.3U patent/CN203759011U/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015070700A1 (en) * | 2013-11-12 | 2015-05-21 | 成都领御生物技术有限公司 | Quantum dot-labeled test strip card |
| WO2015070699A1 (en) * | 2013-11-12 | 2015-05-21 | 成都领御生物技术有限公司 | Quantum dot-labeled test strip card |
| WO2015070704A1 (en) * | 2013-11-13 | 2015-05-21 | 成都领御生物技术有限公司 | Test bar card |
| WO2015070749A1 (en) * | 2013-11-16 | 2015-05-21 | 成都领御生物技术有限公司 | Test strip card |
| CN107287291A (en) * | 2017-06-02 | 2017-10-24 | 华南师范大学 | A kind of double labelling nucleic acid detection method interacted based on g C3N4 and CdTe/CdS quantum dots |
| CN107287291B (en) * | 2017-06-02 | 2021-01-05 | 华南师范大学 | Double-labeled nucleic acid detection method based on interaction of g-C3N4 and CdTe/CdS quantum dots |
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