CN107402294A - A kind of GM food detecting system and method - Google Patents

A kind of GM food detecting system and method Download PDF

Info

Publication number
CN107402294A
CN107402294A CN201710643040.1A CN201710643040A CN107402294A CN 107402294 A CN107402294 A CN 107402294A CN 201710643040 A CN201710643040 A CN 201710643040A CN 107402294 A CN107402294 A CN 107402294A
Authority
CN
China
Prior art keywords
msub
mrow
food
mfrac
experiment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710643040.1A
Other languages
Chinese (zh)
Inventor
刘晓竹
冯钶
童立
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chongqing Aoyun Micro Biological Technology Co Ltd
Original Assignee
Chongqing Aoyun Micro Biological Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chongqing Aoyun Micro Biological Technology Co Ltd filed Critical Chongqing Aoyun Micro Biological Technology Co Ltd
Priority to CN201710643040.1A priority Critical patent/CN107402294A/en
Publication of CN107402294A publication Critical patent/CN107402294A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention provides a kind of GM food detecting system and method,A mobile test end is designed first to be acquired the test result of GM food sample and GM food antibody test solution,The temperature of measurement now simultaneously,Relative humidity and air pressure,Then by the test result,Temperature,Relative humidity and pressure information are by being sent to data terminal,Data terminal is by the information of reception from testing obtained different temperature in advance,Test experience result under varying environment relative humidity and different air pressure conditions is contrasted,Obtain the analysis result of GM food,And the analysis result is sent to mobile test end,Solving existing GM food detection technique needs to carry out in laboratory conditions,The problem of testing result time lag and low detection efficiency,Reach and simplified detection method,Increase detection device portability and improve the purpose of detection accuracy.

Description

A kind of GM food detecting system and method
Technical field
The invention belongs to field of measuring technique, is related to a kind of GM food detecting system and method.
Background technology
Genetically modified organism generally refers to that a kind of biological gene is transferred into another biology using the method for genetic engineering In vivo, so that the biology for receiving alien gene obtains the new features not available for itself, genetically modified organism and transgenosis production The history in the development and application of product existing more than 20 years, especially genetically modified crops and its it was process in recent years by these crops Food achieves swift and violent development, and the fear of the food security thus triggered is also following.In order to select and prevent for consumer The indiscriminate kind of rotation stop gene crops, the detection of GM food have become indispensable food safety detection flow.
The detection of GM food typically has round pcr and immunodetection at present.PCR is PCR The abbreviation of (polymerase chain reaction), it simulates the catalytic action of archaeal dna polymerase in vivo, in vitro Carry out the polymerization and amplification of specific DNA sequence.Round pcr has high degree of specificity and sensitiveness, it is only necessary to which a small amount of DNA is carried out Detection just detected, but to experiment condition require it is higher, its result produces error easily by the interference of factors, the above because Element constrains popularizing for round pcr.Immunodetection is a kind of immunology detection side based on antibody-antigen binding Method, specific biochemical substances are carried out with the technology of qualitative or quantitative analysis.Antigen identifies corresponding antibody by the epitope of antibody surface And combine, it is this to identify the specificity for also making immune detection that there is height.Existing antigen detection is typically by by specialty training The operating personnel of instruction have been come in laboratory environment using purchase or homemade kit and combination such as fluorescence microscope instrument Into.Because the necessary condition of antibody and antigen binding is that antibody comes close to or in contact with antigen, before being dispatched from the factory in existing kit Target antibody in the testing sample covered, testing sample are usually required by the pretreatment such as centrifugation in laboratory, in addition, portion Go-on-go, which is surveyed, to be also needed to buy the antibody crossed of fluorescence labeling to complete.Test experience result is typically to observe the change of color in kit Change, or obtained with fluorescence microscopy fluorescent brightness, achieved finally by manually result is recorded to preserve.Due to inspection Survey personnel need professional training, and testing result is related to the experience of testing staff, have certain subjectivity, cause indirectly anti- Former detection has larger systematic error.
To sum up, the problem of inventor has found, current GM food detection is present has：RCR technical errors are big, experiment is adjusted Religion requires higher, is unfavorable for promoting；Immunodetection complex operation in laboratory environment, it is necessary to carry out, while need to inspection Survey personnel carry out special training, and testing result has subjectivity, and in addition, detection cycle is long, testing result time lag.
The content of the invention
It is an object of the invention to provide a kind of GM food detecting system and method, for solving existing transgenosis food Need to carry out in laboratory conditions in product examine survey technology and the problem of detection efficiency is low.
In order to achieve the above object, the invention provides a kind of mobile test end, including：Test module, for detecting GM food sample is stated, to obtain GM food sample and the test result of GM food antibody test solution reaction；
Environment detection module, for obtaining movement when GM food sample reacts with GM food antibody test solution The information of test lead local environment, the information of mobile test end local environment include temperature, relative humidity and air pressure；
And the first signal transmitting and receiving module, for by the information of mobile test end local environment and test result hair A data terminal is delivered to, and receives the GM food concentration analysis that the data terminal is sent.
Optionally, prepared by the GM food antibody test solution is specially：GM food antibody test solution is filtered Processing, filters out the impurity in the GM food antibody test solution.
Optionally, the mobile test end is also including being used to record the locating module of test position information, believing for inputting The input module of breath and the clock module for recording detection time, the locating module, the input module and the clock Module is connected with the first signal transmitting and receiving module.
Present invention also offers a kind of data terminal, the data terminal includes：
Experiment sample database, for being recorded in multiple different temperatures values, multiple different rh values and multiple differences GM food antibody test solution and GM food sample response data under the conditions of atmospheric pressure value；
Analysis module, for environmental information and test result and the experiment sample data for sending a mobile terminal Data in storehouse are compared, to obtain the concentration of GM food in the GM food sample；And second letter Number transceiver module, the environmental information and test result sent for receiving the mobile test end, and by point of analysis module Analysis result feeds back to the mobile test end.
Optionally, the preparation method of the experiment sample database includes：
Keep experiment rh value and experiment atmospheric pressure value constant, change experimental temperature value by the GM food sample Reacted with the GM food antibody test solution, obtain temperature experiment sample data；
Keep experimental temperature value and experiment atmospheric pressure value constant, change experiment rh value by the GM food sample Reacted with the GM food antibody test solution, obtain humidity experiment sample data；
Keep experimental temperature value and experiment rh value constant, change experiment atmospheric pressure value by the GM food sample Reacted with the GM food antibody test solution, obtain air pressure experimental data；
The temperature experimental data, the humidity experimental data and the air pressure fitting experimental data are obtained into the reaction Experiment sample database.
Optionally, the GM food sample includes the GM food sample of different GM food concentration, described The preparation method of experiment sample database is specially：
Keep RH=50% and P=101.325kPa constant, in T=-50 ° of+0.5n1Under conditions of, by the transgenosis Food samples are reacted with the GM food antibody test solution, obtain the temperature experimental data, n1For experiment number, n1For 0 to 100 integer；
T=20 ° of holding and P=101.325kPa are constant, in RH=(n2- 1) under conditions of × 5%, by the transgenosis Food samples are reacted with the GM food antibody test solution, obtain the humidity experimental data, n2For humidity experiment number, n2 For 1 to 21 integer；
T=20 ° of holding and RH=50% are constant, in P=(901.325+5n3) under conditions of kPa, the transgenosis is eaten Product sample reacts with the GM food antibody test solution, obtains the air pressure experimental data, n3For air pressure experiment number, n3For 0 To 40 integer；
T is the experimental temperature value, and RH is the experiment rh value, and P is the experiment atmospheric pressure value；
The temperature experimental data, the humidity experimental data and the air pressure fitting experimental data are obtained into the reaction Experiment sample database.
Preferably, the data terminal also includes being used for the memory module and use for recording the analysis module analysis result In the display module for showing the analysis result, the memory module is connected with the analysis module signal.
Preferably, present invention also offers a kind of GM food detection method, it includes：
S1：The GM food sample is detected at the mobile test end, obtain the GM food sample with it is described The test result of GM food antibody test solution, and by the ambient temperature value, described of mobile test end detection Environmental wet angle value, the ambient pressure value and the test result are sent to the data terminal；
S2：According to the ambient temperature value, the environmental wet angle value, the ambient pressure value from the experiment sample data The GM food sample response data of various concentrations is obtained in storehouse；
S3：The response data and the test result are analyzed, obtained in the GM food sample The analysis result of GM food concentration, and the analysis result is sent to the mobile test end.
Optionally, the formula of comparative analysis is in the S3：
Wherein, f0For the analysis result, f (T, RH, P) is the experiment sample database, P0For the ambient pressure Value, T0For the ambient temperature value, RH0For the envionmental humidity, T1And T2For in the experiment sample database with T0Phase Two adjacent experimental temperature values, laFor T1And T2Between threshold length, l1And l2Respectively T0Correspond to T1And T2Distance；RH1 And RH2For in the experiment sample database with RH0Two adjacent experiment rh values, lbFor RH1And RH2Between threshold value Length, l3And l4Respectively RH0Correspond to RH1And RH2Distance；P1And P2For in the experiment sample database with P0Adjacent Two experiment atmospheric pressure values, lcFor P1And P2Between threshold length, l5And l6Respectively P0Correspond to P1And P2Distance.
Compared with prior art, a kind of GM food detecting system and method provided by the invention, have beneficial below Effect：
1st, without by extracting GM food sample outside laboratory and the transgenosis being eaten in laboratory conditions Product sample is detected, and portability is increased by using mobile test end, while improves detection efficiency, slow down detection knot The time lag of fruit；
2nd, testing result is obtained by data analysis, more artificial subjective judgement accuracy is high, and substitution manual record achieves, drop Low systematic error, prevents the cheating occurred during manual record, improves the accuracy of GM food detection；
3rd, according to temperature, relative humidity and the pneumatic parameter for influenceing testing result, the broad experiment sample number of threshold value is formulated According to storehouse, the detection to GM food under the conditions of different geographical natural environment and climates is well adapted for, disclosure satisfy that remote Area and the requirement in the severe area of natural conditions；
4th, the personnel without specialty are operable, it is not necessary to carrying out professional technique training using crowd, are reduced using behaviour Make difficulty, be easy to promote the use of；
5th, mobile test end structure is simple, is easy to carry；
6th, mobile test end detection GM food, by mobile communication transmission to data terminal, and then obtain differently The GM food and its concentration data information in area, to disease treatment, medical resource is launched, prevention from suffering from the diseases and scientific research have Certain meaning.
Brief description of the drawings
Fig. 1 is mobile test end provided in an embodiment of the present invention and data terminal structure schematic diagram；
Fig. 2 is experiment sample database preparation method provided in an embodiment of the present invention；
Fig. 3 is GM food detection method flow chart provided in an embodiment of the present invention；
Wherein, 1- test tubes, 21- temperature sensors, 22- relative humidity sensors, 23- baroceptors, 3- signal acquisitions Module, 4- the first signal transmitting and receiving modules, 5- pumping signal modules, 6- secondary signal transceiver modules, 7- analysis modules, 8- experiment samples Database, 9- mobile communication, 10- locating modules, 11- clock modules, 12- input modules, 13- memory modules.
Embodiment
The embodiment of the present invention is described in more detail below in conjunction with schematic diagram.According to description below and Claims, advantages and features of the invention will become apparent from.It should be noted that accompanying drawing is using very simplified form and Using non-accurately ratio, only for the purpose of facilitating and clarifying the purpose of the embodiments of the invention.
Referring to Fig. 1, the embodiments of the invention provide a kind of mobile test end A, for detecting in GM food sample GM food concentration, including：
Test module, for detecting the GM food sample, to obtain GM food sample and GM food The test result of antibody test solution reaction；
Environment detection module, for obtaining movement when GM food sample reacts with GM food antibody test solution The information of test lead local environment, the information of mobile test end local environment include temperature, humidity and air pressure；
And the first signal transmitting and receiving module, for by the information of mobile test end local environment and test result hair A data terminal is delivered to, and receives the GM food concentration analysis that the data terminal is sent.
The test module includes being used for the test tube 1 and signal acquisition module 3 for depositing the GM food antibody test solution, The GM food antibody test solution is used for the GM food concentration for demarcating the GM food sample, signal acquisition Module 3 is used to gather the GM food sample and the test result of GM food antibody test solution reaction；
The environment detection module include be used for measure the mobile test end A ambient temperature values temperature sensor 21, For measuring the humidity sensor 22 of the mobile test end A envionmental humidity values and for measuring the mobile test end A The baroceptor 23 of ambient pressure value；
The test result that first signal transmitting and receiving module 4 gathers signal acquisition module 3, temperature sensor 21 measure The atmospheric pressure value that the envionmental humidity and baroceptor 23 that environment temperature, relative humidity sensor 22 measure measure is sent to number According to end B, and receive the GM food concentration analysis of data terminal B transmissions.
Inventor has found, existing GM food detection technique, typically by gathering GM food sample, then enters Row preservation, then the antibody sample collected is transported in laboratory, to the GM food sample in laboratory environment This is detected, and using this protocol step is various, testing result time lag, detection efficiency are low, and is needed to personnel Professional training is carried out to meet testing requirements.Inventor has found that：Influence the environmental factor master of GM food detection Temperature, envionmental humidity and air pressure when being detection, therefore inventors herein propose in non-laboratory condition (i.e. in different gas Under temperature, envionmental humidity and air pressure conditions) GM food sample is detected, then by by it with testing in advance Test experience result under to different temperature, envionmental humidity and air pressure conditions is contrasted, and obtains GM food detection As a result technical scheme.In order to realize above-mentioned technical proposal, design first a mobile test end A to GM food sample and The test result of the GM food antibody test solution in test tube 1 is acquired, while measures this by temperature sensor 21 When temperature, measure by relative humidity sensor 22 relative humidity now and gas now measured by baroceptor 23 Pressure, is then sent to data terminal B by test result, temperature, relative humidity and pressure information by the first signal transmitting and receiving module, The transmission is carried out in a manner of mobile communication 9, and the mobile communication 9 is, for example, GSM, GPRS, 4G, 5G and/or wifi.
Preferably, prepared by the GM food antibody test solution is specially：The GM food antibody test solution is carried out Filtering and purification processes, the impurity in the GM food antibody test solution is filtered out, and then improve the GM food and resist The concentration of GM food antibody in body test solution, to reduce in GM food antibody test solution unrelated impurity to the shadow of testing result Ring.In practical operation, the GM food antibody test solution in test tube 1 can be prepared according to fixed standard, the test tube 1 Using being detachably connected on the A of mobile test end, when carrying out next GM food pattern detection, change the outfit new dress There is the test tube 1 of the GM food antibody test solution, and then realize the detection to multiple GM food samples.
Preferably, mobile test end A is also including being used to record the locating module 10 of test position information, being detected for recording The clock module 11 of time and the input module 12 for inputting information, it is the locating module 10, the clock module 11, described Input module 12 is connected with the first signal transmitting and receiving module 4 and can realize data transfer.Carrying out GM food inspection While survey, to detection when positional information, temporal information record and data terminal is transferred to by the first signal transmitting and receiving module 4 B.The time and positional information of GM food are recorded, when contributing to data terminal B understandings survey detection site and detection Between, and then contribute to the area for understanding GM food distribution to need to detect the Annual distribution of GM food with tester, it is right The prevention and control of disease, the input of medical resource and scientific research have positive meaning.Input module 12 can be defeated using keyboard Enter, the mode of fingerprint input and/or handwriting input, to the species of detection information such as detection object, the year for needing to mark during detection Age, sex, nationality, health status etc. are inputted and illustrated, help to understand the further information of the GM food, such as When fruit measurement object is people, inspection can be improved with the signature data and/or finger print information of handwriting input tester and detected person Survey objective, the fairness of result.
Preferably, the mobile test end A also includes being used to encourage the GM food antibody in the test tube 1 to try Liquid and the pumping signal module 5 of GM food sample reaction, the pumping signal module 5 are connected with the test tube 1, The GM food antibody test solution and the GM food sample mixing reaction of formation test solution in the test tube 1, it is described The working frequency of pumping signal module 5 is 10Hz to 10MHz.When the GM food in the GM food antibody test solution resists When body instead gives birth to immune response with antibody in the GM food sample, the output signal of the pumping signal in test tube 1 can occur The change of voltage and/or electric current and signal phase, this change turn to the test result, and signal acquisition module 3, which is used to gather, to be tried The change of the voltage and/or electric current and signal phase that occur in pipe 1, signal acquisition module 3 are believed with the first signal transmitting and receiving module 4 Number connection, the first signal transmitting and receiving module 4 are used to the test result being sent to the data terminal.
Traditional detection method is typically combined by the Brownian movement between antibody and antigen molecule, because Blang transports Dynamic speed causes testing result time lag more slowly, and the reaction test solution is persistently swashed using signal driving frequency Encourage, increase the motion liveness of antibody and antigen molecule, it is possible to increase the reaction speed of the reaction test solution, and then reduce Detection time, improve detection efficiency.
The embodiment of the present invention additionally provides a kind of data terminal B, turns base from mobile test end A for receiving, analyzing Because of food antibody test solution and the signal of GM food sample reaction, and analysis result is sent to mobile test end A, the number Include according to terminal：
Experiment sample database 8, the experiment sample database 8 are used to be recorded in multiple different temperatures values, multiple differences GM food antibody test solution and GM food sample response data under the conditions of rh value and different atmospheric pressure values；
Analysis module 7, the analysis module 7 are used for the environmental information and test result for sending a mobile test end A It is compared with the data in the experiment sample database 8, is eaten with obtaining transgenosis in the GM food sample The concentration of product；
And secondary signal transceiver module 6, the secondary signal transceiver module 6 are used to receive the mobile test end A hairs The environmental information and test result sent, and the analysis result of analysis module 6 is fed back into the mobile test end A.
Referring to Fig. 2, the preparation method of the experiment sample database is：
Keep experiment rh value and experiment atmospheric pressure value constant, change experimental temperature value by the GM food sample Reacted with the GM food antibody test solution, obtain temperature experiment sample data；
Keep experimental temperature value and experiment atmospheric pressure value constant, change experiment rh value by the GM food sample Reacted with the GM food antibody test solution, obtain humidity experiment sample data；
Keep experimental temperature value and experiment rh value constant, change experiment atmospheric pressure value by the GM food sample Reacted with the GM food antibody test solution, obtain air pressure experimental data；
The temperature experimental data, the humidity experimental data and the air pressure fitting experimental data are obtained into the reaction Experiment sample database.
Preferably, the GM food sample includes the GM food sample of different GM food concentration, described The preparation of experiment sample database is specially：
Keep RH=50% and P=101.325kPa constant, in T=-50 ° of+0.5n1Under conditions of, by the transgenosis Food samples are reacted with the GM food antibody test solution, obtain the temperature experimental data, n1For experiment number, n1For 0 to 100 integer, temperature is different between each sample in temperature experimental data, and the measurement tolerance between each sample is 0.5 Degree Celsius, take this measurement tolerance to be based on temperature sensor resolution ratio, economic serviceability, experiment number and sample for inventor The consideration of data volume, preferred value is drawn by experimental verification, in a practical situation with GM food and GM food antibody The susceptibility of temperature is considered, can gather more or less temperature experimental data samples, i.e., between each sample Measurement tolerance for 1 degree Celsius ..., 0.4 degree Celsius, 0.3 degree Celsius, 0.2 degree Celsius, 0.1 degree Celsius ....Take this The purpose of sample experiment is recorded under certain relative humidity and air pressure conditions, controls temperature change, observation temperature change is to described GM food sample and the situation of GM food antibody test solution reaction；
T=20 ° of holding and P=101.325kPa are constant, in RH=(n2- 1) under conditions of × 5%, by the transgenosis Food samples are reacted with the GM food antibody test solution, obtain the humidity experimental data, n2For humidity experiment number, n2 For 1 to 21 integer, relative humidity is different between each sample in relative humidity experimental data, the phase between each sample It is 1% to humidity tolerance, takes this relative humidity tolerance to be based on relative humidity sensor resolution ratio, economic and practical for inventor The consideration of property, experiment number and sample data volume, draws preferred value, in a practical situation with GM food by experimental verification The susceptibility of envionmental humidity is considered with GM food antibody, more or less humidity experiments can be gathered Data sample, i.e., the relative humidity tolerance between each sample is 10% ..., 4%, 3%, 2%, 1% ....Take this The purpose of sample experiment is recorded under certain temperature and air pressure conditions, controls relative humidity variations, observes relative humidity variations pair The GM food sample and the situation of GM food antibody test solution reaction；
T=20 ° of holding and RH=50% are constant, in P=(901.325+5n3) under conditions of kPa, the transgenosis is eaten Product sample reacts with the GM food antibody test solution, obtains the air pressure experimental data, n3For air pressure experiment number, n3For 0 To 40 integer, air pressure is different between each sample in air pressure experimental data, and the air pressure tolerance between each sample is 5kPa, this air pressure tolerance is taken to be based on baroceptor resolution ratio, economic serviceability, experiment number and sample number for inventor According to the consideration of amount, preferred value is drawn by experimental verification, in a practical situation with GM food and GM food antibody pair The susceptibility of air pressure considers, and can gather more or less air pressure experimental data samples, i.e., between each sample Air pressure tolerance be 10kPa ..., 4kPa, 3kPa, 2kPa, 1kPa ....The purpose so tested is taken to be recorded on necessarily Under relative humidity and temperature conditionss, control pressure change, observation air pressure change turns base to the GM food sample with described Because of the situation of food antibody test solution reaction；
T is the experimental temperature value, and RH is the experiment rh value, and P is the experiment atmospheric pressure value；By the temperature Experimental data, the humidity experimental data and the air pressure fitting experimental data obtain the reaction experiment sample database.
Preferably, the data terminal B also include being used for recording the memory module 13 of the analysis result of analysis module 7 with And for showing the display module (not marked in figure) of the analysis result, the memory module 13 respectively with the analysis module 7 are connected, due to mobile test end A collection GM food sample from GM food antibody test solution from different ground Area, then mobile test end A will collect information and be sent to data terminal B by telecommunication 9, therefore memory module 13 has not With regional, different geographical environments, different nationalities, Various Seasonal, the big data of all ages and classes GM food sample, this is for disease Disease treatment, medical resource are launched, prevention from suffering from the diseases and scientific research have certain meaning, while testing result is transferred to data terminal B, prevent the cheating occurred during manual record.
Fig. 3 is refer to, present invention also offers a kind of GM food detection method, it includes：
S1：The mobile test end A detects the GM food sample, obtain the GM food sample with it is described The test result of GM food antibody test solution, and by the ambient temperature value, described of mobile test end A detections Environmental wet angle value, the ambient pressure value and the test result are sent to the data terminal B；
S2：According to the ambient temperature value, the environmental wet angle value, the ambient pressure value from the experiment sample data The GM food sample response data of various concentrations is obtained in storehouse 8；
S3：The response data and the test result are analyzed, obtain the GM food sample GM food concentration, and analysis result is sent to the mobile test end A.
Preferably, the formula of comparative analysis is in the S3：
Wherein, f0For the analysis result, f (T, RH, P) is the experiment sample database, P0For the ambient pressure Value, T0For the ambient temperature value, RH0For the envionmental humidity, T1And T2For in the experiment sample database with T0Phase Two adjacent experimental temperature values, laFor T1And T2Between threshold length, l1And l2Respectively T0Correspond to T1And T2Distance；RH1 And RH2For in the experiment sample database with RH0Two adjacent experiment rh values, lbFor RH1And RH2Between threshold value Length, l3And l4Respectively RH0Correspond to RH1And RH2Distance；P1And P2For in the experiment sample database with P0Adjacent Two experiment atmospheric pressure values, lcFor P1And P2Between threshold length, l5And l6Respectively P0Correspond to P1And P2Distance, using adding Weight average algorithm, further increase the accuracy of testing result.
To sum up, in a kind of GM food detecting system provided in an embodiment of the present invention and method, one is designed first Mobile test end is acquired to the test result of GM food sample and GM food antibody test solution, while is measured now Temperature, relative humidity and air pressure, then by test result, temperature, relative humidity and pressure information by be sent to data end The information of reception is obtained the detection under different temperature, envionmental humidity and air pressure conditions by end, data terminal from experiment in advance Experimental result is contrasted, and obtains GM food testing result, and testing result is sent into mobile test end, is solved existing Have need to carry out in laboratory conditions in GM food detection technique, testing result time lag and detection efficiency be low Problem, reach and simplified detection method, increase detection device portability and the purpose for improving detection accuracy.
The preferred embodiments of the present invention are above are only, any restrictions effect is not played to the present invention.Belonging to any Those skilled in the art, in the range of technical scheme is not departed from, to the invention discloses technical scheme and Technology contents make the variation such as any type of equivalent substitution or modification, belong to the content without departing from technical scheme, still Belong within protection scope of the present invention.

Claims (10)

  1. A kind of 1. mobile test end, for detecting the GM food concentration in GM food sample, it is characterised in that described Mobile test end includes：
    Test module, for detecting the GM food sample, to obtain GM food sample and GM food antibody The test result of test solution reaction；
    Environment detection module, for obtaining mobile test when GM food sample reacts with GM food antibody test solution The information of local environment is held, the information of mobile test end local environment includes temperature, relative humidity and air pressure；
    And the first signal transmitting and receiving module, for by the information of mobile test end local environment and test result send to One data terminal, and receive the GM food concentration analysis that the data terminal is sent.
  2. 2. mobile test end as claimed in claim 1, it is characterised in that the GM food antibody test solution prepares specific bag Include：
    Filtration treatment and purification processes are carried out to the GM food antibody test solution, to filter out the GM food antibody Impurity in test solution.
  3. 3. mobile test end as claimed in claim 1, it is characterised in that the mobile test end also includes：
    For recording the locating module of test position information；
    For recording the clock module of detection time；
    And for inputting the input module of information.
  4. 4. a kind of data terminal, it is characterised in that the data terminal includes：
    Experiment sample database, for being recorded in multiple different temperatures values, multiple different rh values and multiple different air pressure GM food antibody test solution and GM food sample response data under the conditions of value；
    Analysis module, for environmental information and test result and the experiment sample database for sending a mobile test end In data be compared, to obtain the concentration of GM food in the GM food sample；
    And secondary signal transceiver module, the environmental information and test result sent for receiving the mobile test end, and The analysis result of analysis module is fed back into the mobile test end.
  5. 5. data terminal as claimed in claim 4, it is characterised in that the preparation method of the experiment sample database includes：
    Keep experiment rh value and experiment atmospheric pressure value constant, change experimental temperature value by the GM food sample and institute The reaction of GM food antibody test solution is stated, obtains temperature experiment sample data；
    Keep experimental temperature value and experiment atmospheric pressure value constant, change experiment rh value by the GM food sample and institute The reaction of GM food antibody test solution is stated, obtains humidity experiment sample data；
    Keep experimental temperature value and experiment rh value constant, change experiment atmospheric pressure value by the GM food sample and institute The reaction of GM food antibody test solution is stated, obtains air pressure experimental data；And
    The temperature experimental data, the humidity experimental data and the air pressure fitting experimental data are obtained into the reaction experiment Sample database.
  6. 6. data terminal as claimed in claim 5, it is characterised in that the GM food sample is eaten including different transgenosis The GM food sample of product concentration, the preparation method of the experiment sample database are specially：
    Keep RH=50% and P=101.325kPa constant, in T=-50 ° of+0.5n1Under conditions of, by the GM food Sample reacts with the GM food antibody test solution, obtains the temperature experimental data, n1For experiment number, n1For 0 to 100 Integer；
    T=20 ° of holding and P=101.325kPa are constant, in RH=(n2- 1) under conditions of × 5%, by the GM food Sample reacts with the GM food antibody test solution, obtains the humidity experimental data, n2For humidity experiment number, n2For 1 to 21 integer；
    T=20 ° of holding and RH=50% are constant, in P=(901.325+5n3) under conditions of kPa, by the GM food sample This reacts with the GM food antibody test solution, obtains the air pressure experimental data, n3For air pressure experiment number, n3For 0 to 40 Integer；
    T is the experimental temperature value, and RH is the experiment rh value, and P is the experiment atmospheric pressure value；
    The temperature experimental data, the humidity experimental data and the air pressure fitting experimental data are obtained into the reaction experiment Sample database.
  7. 7. data terminal as claimed in claim 4, it is characterised in that the data terminal also includes：
    For recording the memory module of the analysis module analysis result and display module for showing the analysis result, The memory module is connected with the analysis module signal.
  8. 8. a kind of GM food concentration detection system, it is characterised in that the GM food detecting system includes：
    Mobile test end described in claim any one of 1-3；And any described data terminals of claim 4-7.
  9. 9. a kind of GM food detection method, it is characterised in that the GM food detection method includes：
    S1：The GM food sample is detected at mobile test end, obtains GM food sample and is tried with GM food antibody The test result of liquid reaction, and the information of mobile test end local environment and the test result are sent to data end End；
    S2：Turning for various concentrations is obtained from the experiment sample database according to the information of mobile test end local environment Gene modified food sample response data；
    S3：The GM food sample response data of the various concentrations is analyzed with the test result, obtained The analysis result of GM food concentration in the GM food sample, and the analysis result is sent to the mobile survey Try end.
  10. A kind of 10. GM food detection method as claimed in claim 9, it is characterised in that comparative analysis in the S3 Formula is：
    <mrow> <mfrac> <mn>1</mn> <mn>3</mn> </mfrac> <mrow> <mo>(</mo> <mo>(</mo> <mfrac> <msub> <mi>l</mi> <mn>1</mn> </msub> <msub> <mi>l</mi> <mi>a</mi> </msub> </mfrac> <mo>)</mo> <msub> <mi>g</mi> <mn>1</mn> </msub> <mo>+</mo> <mo>(</mo> <mfrac> <msub> <mi>l</mi> <mn>2</mn> </msub> <msub> <mi>l</mi> <mi>a</mi> </msub> </mfrac> <mo>)</mo> <msub> <mi>g</mi> <mn>2</mn> </msub> <mo>)</mo> </mrow> <mo>+</mo> <mfrac> <mn>1</mn> <mn>3</mn> </mfrac> <mrow> <mo>(</mo> <mo>(</mo> <mfrac> <msub> <mi>l</mi> <mn>3</mn> </msub> <msub> <mi>l</mi> <mi>b</mi> </msub> </mfrac> <mo>)</mo> <msub> <mi>g</mi> <mn>3</mn> </msub> <mo>+</mo> <mo>(</mo> <mfrac> <msub> <mi>l</mi> <mn>4</mn> </msub> <msub> <mi>l</mi> <mi>b</mi> </msub> </mfrac> <mo>)</mo> <msub> <mi>g</mi> <mn>4</mn> </msub> <mo>)</mo> </mrow> <mo>+</mo> <mfrac> <mn>1</mn> <mn>3</mn> </mfrac> <mrow> <mo>(</mo> <mo>(</mo> <mfrac> <msub> <mi>l</mi> <mn>5</mn> </msub> <msub> <mi>l</mi> <mi>c</mi> </msub> </mfrac> <mo>)</mo> <msub> <mi>g</mi> <mn>5</mn> </msub> <mo>+</mo> <mo>(</mo> <mfrac> <msub> <mi>l</mi> <mn>6</mn> </msub> <msub> <mi>l</mi> <mi>c</mi> </msub> </mfrac> <mo>)</mo> <msub> <mi>g</mi> <mn>6</mn> </msub> <mo>)</mo> </mrow> <mo>=</mo> <msub> <mi>f</mi> <mn>0</mn> </msub> </mrow>
    <mrow> <msub> <mi>g</mi> <mn>1</mn> </msub> <mo>=</mo> <mfrac> <mn>1</mn> <mn>4</mn> </mfrac> <mrow> <mo>(</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>)</mo> </mrow> </mrow>
    <mrow> <msub> <mi>g</mi> <mn>2</mn> </msub> <mo>=</mo> <mfrac> <mn>1</mn> <mn>4</mn> </mfrac> <mrow> <mo>(</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>)</mo> </mrow> </mrow>
    <mrow> <msub> <mi>g</mi> <mn>3</mn> </msub> <mo>=</mo> <mfrac> <mn>1</mn> <mn>4</mn> </mfrac> <mrow> <mo>(</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>)</mo> </mrow> </mrow>
    <mrow> <msub> <mi>g</mi> <mn>4</mn> </msub> <mo>=</mo> <mfrac> <mn>1</mn> <mn>4</mn> </mfrac> <mrow> <mo>(</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>)</mo> </mrow> </mrow>
    <mrow> <msub> <mi>g</mi> <mn>5</mn> </msub> <mo>=</mo> <mfrac> <mn>1</mn> <mn>4</mn> </mfrac> <mrow> <mo>(</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>)</mo> </mrow> </mrow>
    <mrow> <msub> <mi>g</mi> <mn>6</mn> </msub> <mo>=</mo> <mfrac> <mn>1</mn> <mn>4</mn> </mfrac> <mrow> <mo>(</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>)</mo> </mrow> </mrow>
    Wherein, f0For the analysis result, f (T, RH, P) is the experiment sample database, P0For the ambient pressure value, T0 For the ambient temperature value, RH0For the envionmental humidity, T1And T2For in the experiment sample database with T0Adjacent Two experimental temperature values, laFor T1And T2Between threshold length, l1And l2Respectively T0Correspond to T1And T2Distance；RH1With RH2For in the experiment sample database with RH0Two adjacent experiment rh values, lbFor RH1And RH2Between threshold value length Degree, l3And l4Respectively RH0Correspond to RH1And RH2Distance；P1And P2For in the experiment sample database with P0Adjacent two Individual experiment atmospheric pressure value, lcFor P1And P2Between threshold length, l5And l6Respectively P0Correspond to P1And P2Distance.
CN201710643040.1A 2017-07-31 2017-07-31 A kind of GM food detecting system and method Pending CN107402294A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710643040.1A CN107402294A (en) 2017-07-31 2017-07-31 A kind of GM food detecting system and method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710643040.1A CN107402294A (en) 2017-07-31 2017-07-31 A kind of GM food detecting system and method

Publications (1)

Publication Number Publication Date
CN107402294A true CN107402294A (en) 2017-11-28

Family

ID=60401799

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710643040.1A Pending CN107402294A (en) 2017-07-31 2017-07-31 A kind of GM food detecting system and method

Country Status (1)

Country Link
CN (1) CN107402294A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108427287A (en) * 2018-04-18 2018-08-21 遵义市产品质量检验检测院 A kind of intelligence food quality detection robot

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105572110A (en) * 2014-11-04 2016-05-11 三星电子株式会社 A method of and an apparatus for measuring biometric information

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105572110A (en) * 2014-11-04 2016-05-11 三星电子株式会社 A method of and an apparatus for measuring biometric information

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108427287A (en) * 2018-04-18 2018-08-21 遵义市产品质量检验检测院 A kind of intelligence food quality detection robot

Similar Documents

Publication Publication Date Title
US11257215B2 (en) Systems and methods for collecting and transmitting assay results
US9686395B2 (en) Apparatus and method for point-of-collection measurement of a biomolecular reaction
US20130080071A1 (en) Systems and methods for sample processing and analysis
US10919037B2 (en) Systems and apparatus for detecting compounds in human biological samples
CN109789417A (en) Analysis system and method for test sample
CN103698512A (en) Application of mycobacterium tuberculosis proteins in preparation of products used for diagnosis of latent tuberculosis infection
Costa et al. Exploratory study on application of MALDI-TOF-MS to detect SARS-CoV-2 infection in human saliva
CN102323419A (en) Kit and detection method for quantitative determination of digoxin
JP2016188862A (en) Biomarker monitoring system
CN107402294A (en) A kind of GM food detecting system and method
CN107449910A (en) A kind of virus antigen detection system and method
CN107192825A (en) A kind of antiviral antibody detecting system and method
CN107328924A (en) A kind of microbial metabolic products detecting system and method
CN107219353A (en) A kind of antigen nucleic acid detecting system and method
CN107463794A (en) A kind of protein antibody detecting system and method
CN107255715A (en) A kind of microorganism detection system and method
CN107462727A (en) A kind of proteantigen detecting system and method
CN108133754B (en) The forecasting system of bleeding risk after a kind of thrombolysis
CN107356739A (en) One organic molecular species detecting system and method
CN107367604A (en) A kind of nucleic acid antibody detecting system and method
CN106290272A (en) A kind of method of real-time detection Atmospheric particulates bio-toxicity
CN107389914A (en) A kind of drug residue detecting system and method
US20220104726A1 (en) Method and devices for detecting viruses and bacterial pathogens
CN107271659A (en) A kind of metal ion detection system and method
CN110099747A (en) Method and analysis system for test sample

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20171128