CN107328924A - A kind of microbial metabolic products detecting system and method - Google Patents

A kind of microbial metabolic products detecting system and method Download PDF

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Publication number
CN107328924A
CN107328924A CN201710642124.3A CN201710642124A CN107328924A CN 107328924 A CN107328924 A CN 107328924A CN 201710642124 A CN201710642124 A CN 201710642124A CN 107328924 A CN107328924 A CN 107328924A
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CN
China
Prior art keywords
msub
mrow
metabolic products
microbial metabolic
mfrac
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CN201710642124.3A
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Inventor
刘晓竹
冯钶
童立
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Chongqing Aoyun Micro Biological Technology Co Ltd
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Chongqing Aoyun Micro Biological Technology Co Ltd
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Priority to CN201710642124.3A priority Critical patent/CN107328924A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding

Abstract

The invention provides a kind of microbial metabolic products detecting system and method, a mobile test end is designed first to be acquired the test result of microbial metabolic products sample and microbial metabolic products antibody test solution, temperature now is measured simultaneously, relative humidity and air pressure, then by the test result, temperature, relative humidity and pressure information are by being sent to data terminal, data terminal is by the information of reception from testing obtained different temperature in advance, test experience result under varying environment relative humidity and different air pressure conditions is contrasted, obtain the analysis result of microbial metabolic products, and the analysis result is sent to mobile test end, solving existing microbial metabolic products detection technique needs to carry out in laboratory conditions, the problem of testing result time lag and low detection efficiency, reach and simplified detection method, increase detection device portability and improve the purpose of detection accuracy.

Description

A kind of microbial metabolic products detecting system and method
Technical field
The invention belongs to field of measuring technique, it is related to a kind of microbial metabolic products detecting system and method.
Background technology
Microbial metabolic products include toxin, mycin, polysaccharide, lipid, vitamin, nucleotides etc., the daily life to the mankind Work has great significance, and it is broad that they are related to classification.
The detection of microbial metabolic products generally use microscope observation and immunological detection, microscope observation by Equipment and laboratory condition limitation, generally require technical professional in laboratory conditions using microscope to microorganism generation Thank to product sample to be observed and analyzed;Immune detection is a kind of immunological detection method based on antibody-antigen binding, to spy Determine the technology that biochemical substances carry out qualitative or quantitative analysis.Antigen is recognized by the epitope of antibody surface to be corresponded to antibody and combines, It is this to recognize the specificity for also making immune detection that there is height.
The detection of existing antigen be typically by the operating personnel Jing Guo professional training laboratory environment using buying or Homemade kit simultaneously combines such as fluorescence microscope instrument to complete.Because the necessary condition of antibody and antigen binding is antibody Come close to or in contact with antigen, there is target antibody in the testing sample covered before dispatching from the factory in existing kit, testing sample leads to Often need in laboratory by the pretreatment such as centrifugation, in addition, part detection is also needed to buy the antibody crossed of fluorescence labeling and Into.Test experience result is typically to observe the change of color in kit, or is obtained with fluorescence microscopy fluorescent brightness Take, achieved finally by manually result is recorded to preserve.Because testing staff needs professional training, testing result and detection people The experience of member is related, and with certain subjectivity, the detection of antigen is caused indirectly larger systematic error.
To sum up, inventor has found, the problem of current immune detection is present has:Complex operation, the detection of many antigens is all needed To be carried out in laboratory environment;Need to carry out special training to testing staff simultaneously, testing result has subjectivity;Except this it Outside, detection cycle length, testing result time lag.
The content of the invention
It is an object of the invention to provide a kind of microbial metabolic products detecting system and method, for solving existing micro- life Need to carry out in laboratory conditions in thing metabolite detection technique and the problem of detection efficiency is low.
In order to achieve the above object, the invention provides a kind of mobile test end, including:Test module, for detecting Microbial metabolic products sample is stated, to obtain the survey that microbial metabolic products sample reacts with microbial metabolic products antibody test solution Test result;
Environment detection module, when being reacted for obtaining microbial metabolic products sample with microbial metabolic products antibody test solution The information of mobile test end local environment, the information of mobile test end local environment include temperature, relative humidity and Air pressure;
And the first signal transmitting and receiving module, for by the information of mobile test end local environment and test result hair A data terminal is delivered to, and receives the microbial metabolic products concentration analysis that the data terminal is sent.
Optionally, prepared by the microbial metabolic products antibody test solution is specially:To microbial metabolic products antibody test solution Filtration treatment is carried out, the impurity in the microbial metabolic products antibody test solution is filtered out.
Optionally, the mobile test end is also including being used to record the locating module of test position information, believing for inputting The input module of breath and the clock module for recording detection time, the locating module, the input module and the clock Module is connected with the first signal transmitting and receiving module.
Present invention also offers a kind of data terminal, the data terminal includes:
Experiment sample database, for recording in multiple different temperatures values, multiple different rh values and multiple differences Microbial metabolic products antibody test solution and microbial metabolic products sample response data under the conditions of atmospheric pressure value;
Analysis module, for environmental information and test result and the experiment sample data for sending a mobile terminal Data in storehouse are compared, to obtain the concentration of microbial metabolic products in the microbial metabolic products sample;With And secondary signal transceiver module, for receiving environmental information and test result that the mobile test end is sent, and will analysis The analysis result of module feeds back to the mobile test end.
Optionally, the preparation method of the experiment sample database includes:
Keep experiment rh value and experiment atmospheric pressure value constant, change experimental temperature value by the microbial metabolic products Sample reacts with the microbial metabolic products antibody test solution, obtains temperature experiment sample data;
Keep experimental temperature value and experiment atmospheric pressure value constant, change experiment rh value by the microbial metabolic products Sample reacts with the microbial metabolic products antibody test solution, obtains humidity experiment sample data;
Keep experimental temperature value and experiment rh value constant, change experiment atmospheric pressure value by the microbial metabolic products Sample reacts with the microbial metabolic products antibody test solution, obtains air pressure experimental data;
The temperature experimental data, the humidity experimental data and the air pressure fitting experimental data are obtained into the reaction Experiment sample database.
Optionally, the microbial metabolic products sample includes the microbial metabolism production of different microorganisms Metabolites Concentration Thing sample, the preparation method of the experiment sample database is specially:
Keep RH=50% and P=101.325kPa constant, in T=-50 ° of+0.5n1Under conditions of, by the microorganism Metabolite sample reacts with the microbial metabolic products antibody test solution, obtains the temperature experimental data, n1For experiment time Number, n1For 0 to 100 integer;
T=20 ° of holding and P=101.325kPa are constant, in RH=(n2- 1) under conditions of × 5%, by the microorganism Metabolite sample reacts with the microbial metabolic products antibody test solution, obtains the humidity experimental data, n2It is real for humidity Test number of times, n2For 1 to 21 integer;
T=20 ° of holding and RH=50% are constant, in P=(901.325+5n3) under conditions of kPa, by the microorganism generation Thank to product sample to react with the microbial metabolic products antibody test solution, obtain the air pressure experimental data, n3Tested for air pressure Number of times, n3For 0 to 40 integer;
T is the experimental temperature value, and RH is the experiment rh value, and P is the experiment atmospheric pressure value;
The temperature experimental data, the humidity experimental data and the air pressure fitting experimental data are obtained into the reaction Experiment sample database.
Preferably, the data terminal also includes the memory module and use for being used to record the analysis module analysis result In the display module for showing the analysis result, the memory module is connected with the analysis module signal.
Preferably, present invention also offers a kind of microbial metabolic products detection method, it includes:
S1:The microbial metabolic products sample is detected at the mobile test end, obtains the microbial metabolic products sample Originally with the test result of the microbial metabolic products antibody test solution, and the environment that the mobile test end is detected Temperature value, the environmental wet angle value, the ambient pressure value and the test result are sent to the data terminal;
S2:According to the ambient temperature value, the environmental wet angle value, the ambient pressure value from the experiment sample data The microbial metabolic products sample response data of various concentrations is obtained in storehouse;
S3:The response data is analyzed with the test result, the microbial metabolic products sample is obtained The analysis result of microbial metabolic products concentration in this, and the analysis result is sent to the mobile test end.
Optionally, the formula of comparative analysis is in the S3:
Wherein, f0For the analysis result, f (T, RH, P) is the experiment sample database, P0For the ambient pressure Value, T0For the ambient temperature value, RH0For the envionmental humidity, T1And T2For in the experiment sample database with T0Phase Two adjacent experimental temperature values, laFor T1And T2Between threshold length, l1And l2Respectively T0Correspond to T1And T2Distance;RH1 And RH2For in the experiment sample database with RH0Two adjacent experiment rh values, lbFor RH1And RH2Between threshold value Length, l3And l4Respectively RH0Correspond to RH1And RH2Distance;P1And P2For in the experiment sample database with P0Adjacent Two experiment atmospheric pressure values, lcFor P1And P2Between threshold length, l5And l6Respectively P0Correspond to P1And P2Distance.
Compared with prior art, the present invention is provided a kind of microbial metabolic products detecting system and method, with following Beneficial effect:
1st, without by the outdoor extraction microbial metabolic products sample of experiment and in laboratory conditions to micro- life Thing metabolite sample is detected, portability is increased by using mobile test end, while improving detection efficiency, is slowed down Time lag of testing result;
2nd, testing result is obtained by data analysis, more artificial subjective judgement accuracy is high, substitution manual record is achieved, drop Low systematic error, prevents the cheating occurred during manual record, improves the accurate of microbial metabolic products detection Property;
3rd, according to temperature, relative humidity and the pneumatic parameter of influence testing result, the broad experiment sample number of threshold value is formulated According to storehouse, the detection to microbial metabolic products under the conditions of different geographical natural environment and climates is well adapted for, disclosure satisfy that inclined Remote area and the use requirement in the severe area of natural conditions;
4th, the personnel without specialty are operable, it is not necessary to carrying out professional technique training using crowd, reduced using behaviour Make difficulty, be easy to promote the use of;
5th, mobile test end structure is simple, is easy to carry;
6th, mobile test end detection microbial metabolic products, by mobile communication transmission to data terminal, and then are obtained not With the microbial metabolic products and its concentration data information in area, to disease treatment, medical resource dispensing, prevention from suffering from the diseases and science Research has certain meaning.
Brief description of the drawings
Fig. 1 is mobile test end provided in an embodiment of the present invention and data terminal structure schematic diagram;
Fig. 2 is experiment sample database preparation method provided in an embodiment of the present invention;
Fig. 3 is microbial metabolic products detection method flow chart provided in an embodiment of the present invention;
Wherein, 1- test tubes, 21- temperature sensors, 22- relative humidity sensors, 23- baroceptors, 3- signal acquisitions Module, 4- the first signal transmitting and receiving modules, 5- pumping signal modules, 6- secondary signal transceiver modules, 7- analysis modules, 8- experiment samples Database, 9- mobile communication, 10- locating modules, 11- clock modules, 12- input modules, 13- memory modules.
Embodiment
The embodiment of the present invention is described in more detail below in conjunction with schematic diagram.According to description below and Claims, advantages and features of the invention will become apparent from.It should be noted that, accompanying drawing uses very simplified form and equal Using non-accurately ratio, only for the purpose of facilitating and clarifying the purpose of the embodiments of the invention.
Referring to Fig. 1, the embodiments of the invention provide a kind of mobile test end A, for detecting microbial metabolic products sample Microbial metabolic products concentration in this, including:
Test module, for detecting the microbial metabolic products sample, with obtain microbial metabolic products sample with it is micro- The test result of biological metabolic product antibody test solution reaction;
Environment detection module, when being reacted for obtaining microbial metabolic products sample with microbial metabolic products antibody test solution The information of mobile test end local environment, the information of mobile test end local environment includes temperature, humidity and air pressure;
And the first signal transmitting and receiving module, for by the information of mobile test end local environment and test result hair A data terminal is delivered to, and receives the microbial metabolic products concentration analysis that the data terminal is sent.
The test module includes the test tube 1 and signal acquisition mould for being used to deposit the microbial metabolic products antibody test solution Block 3, the microbial metabolic products antibody test solution is used for the microbial metabolism for demarcating the microbial metabolic products sample Production concentration, signal acquisition module 3 is used to gather the microbial metabolic products sample and the microbial metabolic products antibody The test result of test solution reaction;
The environment detection module include be used for measure the mobile test end A ambient temperature values temperature sensor 21, For measuring the humidity sensor 22 of the mobile test end A envionmental humidity values and for measuring the mobile test end A The baroceptor 23 of ambient pressure value;
The test result that first signal transmitting and receiving module 4 gathers signal acquisition module 3, temperature sensor 21 are measured The atmospheric pressure value that the envionmental humidity and baroceptor 23 that environment temperature, relative humidity sensor 22 are measured are measured is sent to number According to end B, and receive the microbial metabolic products concentration analysis that data terminal B sends and come.
Inventor's discovery, existing microbial metabolic products detection technique, typically by gathering microbial metabolic products sample This, then carries out preservation, then the antibody sample collected is transported in laboratory, to micro- life in laboratory environment Thing metabolite sample detected, using this protocol step is various, testing result time lag, detection efficiency it is low, and And need to carry out personnel professional training to meet detection requirement.Inventor has found that:Influence microbial metabolic products Temperature, envionmental humidity and air pressure when the environmental factor of detection is mainly detection, therefore inventors herein propose in non-experiment Room condition (i.e. under different temperature, envionmental humidity and air pressure conditions) detects to microbial metabolic products sample, so Afterwards by the way that itself and experiment in advance to be obtained to the test experience result progress pair under different temperature, envionmental humidity and air pressure conditions Than obtaining the technical scheme of microbial metabolic products testing result.In order to realize above-mentioned technical proposal, a movement is designed first Test lead A enters to the test result of the microbial metabolic products antibody test solution in microbial metabolic products sample and test tube 1 Row collection, while measuring temperature now by temperature sensor 21, now relative being measured by relative humidity sensor 22 Humidity and air pressure now is measured by baroceptor 23, then led to test result, temperature, relative humidity and pressure information Cross the first signal transmitting and receiving module and be sent to data terminal B, the transmission is carried out in the way of mobile communication 9, the mobile communication 9 For example, GSM, GPRS, 4G, 5G and/or wifi.
Preferably, prepared by the microbial metabolic products antibody test solution is specially:To the microbial metabolic products antibody Test solution is filtered and purification processes, filters out the impurity in the microbial metabolic products antibody test solution, and then improve described The concentration of microbial metabolic products antibody in microbial metabolic products antibody test solution, to reduce microbial metabolic products antibody test solution In influence of the unrelated impurity to testing result.In practical operation, the microbial metabolic products antibody in test tube 1 can be tried Liquid is prepared according to fixed standard, and the test tube 1 is next micro- in progress using being detachably connected on the A of mobile test end During biological metabolic product pattern detection, change the outfit the new test tube 1 equipped with the microbial metabolic products antibody test solution, and then realizes Detection to multiple microbial metabolic products samples.
Preferably, mobile test end A is also including being used to record the locating module 10 of test position information, being detected for recording The clock module 11 of time and the input module 12 for inputting information, it is the locating module 10, the clock module 11, described Input module 12 is connected with the first signal transmitting and receiving module 4 and can realize data transfer.Carrying out microbial metabolism production While analyte detection, positional information, temporal information during to detection record and are transferred to data by the first signal transmitting and receiving module 4 Terminal B.The time and positional information of microbial metabolic products are recorded, contributes to data terminal B to understand and surveys detection site And detection time, and then contribute to the area for understanding microbial metabolic products distribution to need detection microbial metabolism production with tester The Annual distribution of thing, the input of prevention and control, medical resource to disease and scientific research have positive meaning.Input module 12 can Be using input through keyboard, fingerprint input and/or handwriting input by the way of, to detection when need mark detection information as detect The species of object, age, sex, nationality, health status etc. are inputted and illustrated, help to understand microbial metabolism production The further information of thing, if measurement object is people, can with the signature data of handwriting input tester and detected person and/or Finger print information, improves objective, the fairness of testing result.
Preferably, the mobile test end A also includes being used to encourage the microbial metabolic products in the test tube 1 to resist The pumping signal module 5 that body test solution reacts with the microbial metabolic products sample, the pumping signal module 5 and the test tube 1 is connected, and the microbial metabolic products antibody test solution in the test tube 1 mixes life with the microbial metabolic products sample Into reaction test solution, the working frequency of the pumping signal module 5 is 10Hz to 10MHz.When the microbial metabolic products antibody When microbial metabolic products antibody in test solution instead gives birth to immune response with antibody in the microbial metabolic products sample, test tube 1 The change of voltage and/or electric current and signal phase can occur for the output signal of interior pumping signal, and this change turns to the test As a result, signal acquisition module 3 is used to gather the change of the voltage and/or electric current and signal phase that occur in test tube 1, and signal is adopted Collection module 3 is connected with the signal of the first signal transmitting and receiving module 4, and the first signal transmitting and receiving module 4 is used to the test result being sent to The data terminal.
Traditional detection method is typically combined by the Brownian movement between antibody and antigen molecule, due to Blang's fortune Dynamic speed causes testing result time lag more slowly, and the reaction test solution is persistently swashed using signal driving frequency Encourage, increase the motion liveness of antibody and antigen molecule, it is possible to increase the reaction speed of the reaction test solution, and then reduce Detection time, improves detection efficiency.
The embodiment of the present invention additionally provides a kind of data terminal B, for receiving, analyzing micro- life from mobile test end A The signal that thing metabolic product antibody test solution reacts with microbial metabolic products sample, and analysis result is sent to mobile test end A, the data terminal includes:
Experiment sample database 8, the experiment sample database 8 is used to record in multiple different temperatures values, multiple differences Microbial metabolic products antibody test solution and microbial metabolic products sample stoichiometric number under the conditions of rh value and different atmospheric pressure values According to;
Analysis module 7, the analysis module 7 is used for the environmental information and test result for sending a mobile test end A It is compared with the data in the experiment sample database 8, to obtain micro- life in the microbial metabolic products sample The concentration of thing metabolite;
And secondary signal transceiver module 6, the secondary signal transceiver module 6 is for receiving the mobile test end A hairs The environmental information and test result sent, and the analysis result of analysis module 6 is fed back into the mobile test end A.
Referring to Fig. 2, the preparation method of the experiment sample database is:
Keep experiment rh value and experiment atmospheric pressure value constant, change experimental temperature value by the microbial metabolic products Sample reacts with the microbial metabolic products antibody test solution, obtains temperature experiment sample data;
Keep experimental temperature value and experiment atmospheric pressure value constant, change experiment rh value by the microbial metabolic products Sample reacts with the microbial metabolic products antibody test solution, obtains humidity experiment sample data;
Keep experimental temperature value and experiment rh value constant, change experiment atmospheric pressure value by the microbial metabolic products Sample reacts with the microbial metabolic products antibody test solution, obtains air pressure experimental data;
The temperature experimental data, the humidity experimental data and the air pressure fitting experimental data are obtained into the reaction Experiment sample database.
Preferably, the microbial metabolic products sample includes the microbial metabolism production of different microorganisms Metabolites Concentration Thing sample, the preparation of the experiment sample database is specially:
Keep RH=50% and P=101.325kPa constant, in T=-50 ° of+0.5n1Under conditions of, by the microorganism Metabolite sample reacts with the microbial metabolic products antibody test solution, obtains the temperature experimental data, n1For experiment time Number, n1For 0 to 100 integer, temperature is different between each sample in temperature experimental data, the temperature between each sample Tolerance is 0.5 degree Celsius, takes this measurement tolerance to be based on temperature sensor resolution ratio, economic serviceability, experiment for inventor The consideration of number of times and sample data volume, preferred value is drawn by experimental verification, in a practical situation with microbial metabolic products and Microbial metabolic products antibody considers to the susceptibility of temperature, can gather more or less temperature experimental data samples This, i.e., the measurement tolerance between each sample be 1 degree Celsius ..., 0.4 degree Celsius, 0.3 degree Celsius, 0.2 degree Celsius, 0.1 take the photograph Family name's degree ....Take the purpose so tested to be recorded under certain relative humidity and air pressure conditions, control temperature change, see Examine the situation that temperature change is reacted the microbial metabolic products sample and the microbial metabolic products antibody test solution;
T=20 ° of holding and P=101.325kPa are constant, in RH=(n2- 1) under conditions of × 5%, by the microorganism Metabolite sample reacts with the microbial metabolic products antibody test solution, obtains the humidity experimental data, n2It is real for humidity Test number of times, n2For 1 to 21 integer, relative humidity is different between each sample in relative humidity experimental data, each sample Relative humidity tolerance between this is 1%, takes this relative humidity tolerance to be differentiated for inventor based on relative humidity sensor Rate, economic serviceability, the consideration of experiment number and sample data volume, draw preferred value, in a practical situation by experimental verification The susceptibility of envionmental humidity is considered with microbial metabolic products and microbial metabolic products antibody, can be gathered more Many or less humidity experimental data samples, i.e. relative humidity tolerance between each sample be 10% ..., 4%, 3%, 2%th, 1% ....Take the purpose so tested to be recorded under certain temperature and air pressure conditions, control relative humidity variations, Observe the feelings that relative humidity variations are reacted the microbial metabolic products sample and the microbial metabolic products antibody test solution Condition;
T=20 ° of holding and RH=50% are constant, in P=(901.325+5n3) under conditions of kPa, by the microorganism generation Thank to product sample to react with the microbial metabolic products antibody test solution, obtain the air pressure experimental data, n3Tested for air pressure Number of times, n3For 0 to 40 integer, air pressure is different between each sample in air pressure experimental data, the gas between each sample Pressure tolerance is 5kPa, takes this air pressure tolerance to be based on baroceptor resolution ratio, economic serviceability, experiment number for inventor With the consideration of sample data volume, preferred value is drawn by experimental verification, in a practical situation with microbial metabolic products and micro- life Thing metabolic product antibody considers to the susceptibility of air pressure, can gather more or less air pressure experimental data samples, Air pressure tolerance i.e. between each sample is 10kPa ..., 4kPa, 3kPa, 2kPa, 1kPa ....Take what is so tested Purpose is recorded under certain relative humidity and temperature conditionss, controls air pressure change, observation air pressure change is to the microorganism generation Thank to the situation that product sample reacts with the microbial metabolic products antibody test solution;
T is the experimental temperature value, and RH is the experiment rh value, and P is the experiment atmospheric pressure value;By the temperature Experimental data, the humidity experimental data and the air pressure fitting experimental data obtain the reaction experiment sample database.
Preferably, the data terminal B also include be used for record the memory module 13 of the analysis result of analysis module 7 with And for showing the display module (not marked in figure) of the analysis result, the memory module 13 respectively with the analysis module 7 are connected, because the A microbial metabolic products samples gathered in mobile test end come from not with microbial metabolic products antibody test solution With area, then mobile test end A will collect information and data terminal B, therefore memory module 13 are sent to by telecommunication 9 With different regions, different geographical environment, different nationalities, Various Seasonal, all ages and classes microbial metabolic products sample big number According to this is delivered for disease treatment, medical resource, prevention from suffering from the diseases and scientific research have certain meaning, while testing result Data terminal B is transferred to, the cheating occurred during manual record is prevented.
Fig. 3 is refer to, present invention also offers a kind of microbial metabolic products detection method, it includes:
S1:The mobile test end A detects the microbial metabolic products sample, obtains the microbial metabolic products sample Originally with the test result of the microbial metabolic products antibody test solution, and the ring that the mobile test end A is detected Border temperature value, the environmental wet angle value, the ambient pressure value and the test result are sent to the data terminal B;
S2:According to the ambient temperature value, the environmental wet angle value, the ambient pressure value from the experiment sample data The microbial metabolic products sample response data of various concentrations is obtained in storehouse 8;
S3:The response data is analyzed with the test result, the microbial metabolic products sample is obtained This microbial metabolic products concentration, and analysis result is sent to the mobile test end A.
Preferably, the formula of comparative analysis is in the S3:
Wherein, f0For the analysis result, f (T, RH, P) is the experiment sample database, P0For the ambient pressure Value, T0For the ambient temperature value, RH0For the envionmental humidity, T1And T2For in the experiment sample database with T0Phase Two adjacent experimental temperature values, laFor T1And T2Between threshold length, l1And l2Respectively T0Correspond to T1And T2Distance;RH1 And RH2For in the experiment sample database with RH0Two adjacent experiment rh values, lbFor RH1And RH2Between threshold value Length, l3And l4Respectively RH0Correspond to RH1And RH2Distance;P1And P2For in the experiment sample database with P0Adjacent Two experiment atmospheric pressure values, lcFor P1And P2Between threshold length, l5And l6Respectively P0Correspond to P1And P2Distance, using adding Weight average algorithm, further increases the accuracy of testing result.
To sum up, in a kind of microbial metabolic products detecting system and method provided in an embodiment of the present invention, design first One mobile test end is acquired to the test result of microbial metabolic products sample and microbial metabolic products antibody test solution, Temperature, relative humidity and the air pressure of measurement now, then pass through test result, temperature, relative humidity and pressure information simultaneously Data terminal is sent to, the information of reception is obtained different temperature, envionmental humidity and air pressure by data terminal from experiment in advance Under the conditions of test experience result contrasted, obtain microbial metabolic products testing result, and testing result is sent to shifting Dynamic test lead, solve need to carry out in laboratory conditions in existing microbial metabolic products detection technique, testing result when Between delayed and low detection efficiency the problem of, reached simplify detection method, increase detection device portability and improve detection essence The purpose of exactness.
The preferred embodiments of the present invention are above are only, any restriction effect is not played to the present invention.Belonging to any Those skilled in the art, in the range of technical scheme is not departed from, to the invention discloses technical scheme and Technology contents make the variation such as any type of equivalent substitution or modification, belong to the content without departing from technical scheme, still Belong within protection scope of the present invention.

Claims (10)

1. a kind of mobile test end, for detecting the microbial metabolic products concentration in microbial metabolic products sample, its feature It is, the mobile test end includes:
Test module, for detecting the microbial metabolic products sample, to obtain microbial metabolic products sample and microorganism The test result of metabolic product antibody test solution reaction;
Environment detection module, described in obtaining when microbial metabolic products sample reacts with microbial metabolic products antibody test solution The information of mobile test end local environment, the information of mobile test end local environment includes temperature, relative humidity and air pressure;
And the first signal transmitting and receiving module, for by the information and test result of mobile test end local environment send to One data terminal, and receive the microbial metabolic products concentration analysis that the data terminal is sent.
2. mobile test end as claimed in claim 1, it is characterised in that the microbial metabolic products antibody test solution prepares tool Body includes:
Filtration treatment and purification processes are carried out to the microbial metabolic products antibody test solution, to filter out the microbial metabolism Impurity in product antibodies test solution.
3. mobile test end as claimed in claim 1, it is characterised in that the mobile test end also includes:
Locating module for recording test position information;
Clock module for recording detection time;
And for inputting the input module of information.
4. a kind of data terminal, it is characterised in that the data terminal includes:
Experiment sample database, for recording in multiple different temperatures values, multiple different rh values and multiple different air pressure Microbial metabolic products antibody test solution and microbial metabolic products sample response data under the conditions of value;
Analysis module, for environmental information and test result and the experiment sample database for sending a mobile test end In data be compared, to obtain the concentration of microbial metabolic products in the microbial metabolic products sample;
And secondary signal transceiver module, for receiving environmental information and test result that the mobile test end is sent, and The analysis result of analysis module is fed back into the mobile test end.
5. data terminal as claimed in claim 4, it is characterised in that the preparation method of the experiment sample database includes:
Keep experiment rh value and experiment atmospheric pressure value constant, change experimental temperature value by the microbial metabolic products sample Reacted with the microbial metabolic products antibody test solution, obtain temperature experiment sample data;
Keep experimental temperature value and experiment atmospheric pressure value constant, change experiment rh value by the microbial metabolic products sample Reacted with the microbial metabolic products antibody test solution, obtain humidity experiment sample data;
Keep experimental temperature value and experiment rh value constant, change experiment atmospheric pressure value by the microbial metabolic products sample Reacted with the microbial metabolic products antibody test solution, obtain air pressure experimental data;And
The temperature experimental data, the humidity experimental data and the air pressure fitting experimental data are obtained into the reaction experiment Sample database.
6. data terminal as claimed in claim 5, it is characterised in that the microbial metabolic products sample includes different micro- lifes The microbial metabolic products sample of thing Metabolites Concentration, the preparation method of the experiment sample database is specially:
Keep RH=50% and P=101.325kPa constant, in T=-50 ° of+0.5n1Under conditions of, by the microbial metabolism Product sample reacts with the microbial metabolic products antibody test solution, obtains the temperature experimental data, n1For experiment number, n1 For 0 to 100 integer;
T=20 ° of holding and P=101.325kPa are constant, in RH=(n2- 1) under conditions of × 5%, by the microbial metabolism Product sample reacts with the microbial metabolic products antibody test solution, obtains the humidity experimental data, n2For humidity experiment time Number, n2For 1 to 21 integer;
T=20 ° of holding and RH=50% are constant, in P=(901.325+5n3) under conditions of kPa, the microbial metabolism is produced Thing sample reacts with the microbial metabolic products antibody test solution, obtains the air pressure experimental data, n3For air pressure experiment number, n3For 0 to 40 integer;
T is the experimental temperature value, and RH is the experiment rh value, and P is the experiment atmospheric pressure value;
The temperature experimental data, the humidity experimental data and the air pressure fitting experimental data are obtained into the reaction experiment Sample database.
7. data terminal as claimed in claim 4, it is characterised in that the data terminal also includes:
For recording the memory module of the analysis module analysis result and display module for showing the analysis result, The memory module is connected with the analysis module signal.
8. a kind of microbial metabolic products concentration detection system, it is characterised in that the microbial metabolic products detecting system bag Include:
Mobile test end described in claim any one of 1-3;And any described data terminals of claim 4-7.
9. a kind of microbial metabolic products detection method, it is characterised in that the microbial metabolic products detection method includes:
S1:The microbial metabolic products sample is detected at mobile test end, obtains microbial metabolic products sample and microorganism generation Thank to the test result of product antibodies test solution reaction, and the information of mobile test end local environment and the test result are sent out Give a data terminal;
S2:The micro- of various concentrations is obtained from the experiment sample database according to the information of mobile test end local environment Biological metabolic product sample response data;
S3:The microbial metabolic products sample response data of the various concentrations and the test result are analyzed, The analysis result of microbial metabolic products concentration in the microbial metabolic products sample is obtained, and the analysis result is sent To the mobile test end.
10. a kind of microbial metabolic products detection method as claimed in claim 9, it is characterised in that to score in the S3 The formula of analysis is:
<mrow> <mfrac> <mn>1</mn> <mn>3</mn> </mfrac> <mrow> <mo>(</mo> <mo>(</mo> <mfrac> <msub> <mi>l</mi> <mn>1</mn> </msub> <msub> <mi>l</mi> <mi>a</mi> </msub> </mfrac> <mo>)</mo> <msub> <mi>g</mi> <mn>1</mn> </msub> <mo>+</mo> <mo>(</mo> <mfrac> <msub> <mi>l</mi> <mn>2</mn> </msub> <msub> <mi>l</mi> <mi>a</mi> </msub> </mfrac> <mo>)</mo> <msub> <mi>g</mi> <mn>2</mn> </msub> <mo>)</mo> </mrow> <mo>+</mo> <mfrac> <mn>1</mn> <mn>3</mn> </mfrac> <mrow> <mo>(</mo> <mo>(</mo> <mfrac> <msub> <mi>l</mi> <mn>3</mn> </msub> <msub> <mi>l</mi> <mi>b</mi> </msub> </mfrac> <mo>)</mo> <msub> <mi>g</mi> <mn>3</mn> </msub> <mo>+</mo> <mo>(</mo> <mfrac> <msub> <mi>l</mi> <mn>4</mn> </msub> <msub> <mi>l</mi> <mi>b</mi> </msub> </mfrac> <mo>)</mo> <msub> <mi>g</mi> <mn>4</mn> </msub> <mo>)</mo> </mrow> <mo>+</mo> <mfrac> <mn>1</mn> <mn>3</mn> </mfrac> <mrow> <mo>(</mo> <mo>(</mo> <mfrac> <msub> <mi>l</mi> <mn>5</mn> </msub> <msub> <mi>l</mi> <mi>c</mi> </msub> </mfrac> <mo>)</mo> <msub> <mi>g</mi> <mn>5</mn> </msub> <mo>+</mo> <mo>(</mo> <mfrac> <msub> <mi>l</mi> <mn>6</mn> </msub> <msub> <mi>l</mi> <mi>c</mi> </msub> </mfrac> <mo>)</mo> <msub> <mi>g</mi> <mn>6</mn> </msub> <mo>)</mo> </mrow> <mo>=</mo> <msub> <mi>f</mi> <mn>0</mn> </msub> </mrow>
<mrow> <msub> <mi>g</mi> <mn>1</mn> </msub> <mo>=</mo> <mfrac> <mn>1</mn> <mn>4</mn> </mfrac> <mrow> <mo>(</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>)</mo> </mrow> </mrow>
<mrow> <msub> <mi>g</mi> <mn>2</mn> </msub> <mo>=</mo> <mfrac> <mn>1</mn> <mn>4</mn> </mfrac> <mrow> <mo>(</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>)</mo> </mrow> </mrow>
<mrow> <msub> <mi>g</mi> <mn>3</mn> </msub> <mo>=</mo> <mfrac> <mn>1</mn> <mn>4</mn> </mfrac> <mrow> <mo>(</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>)</mo> </mrow> </mrow>
<mrow> <msub> <mi>g</mi> <mn>4</mn> </msub> <mo>=</mo> <mfrac> <mn>1</mn> <mn>4</mn> </mfrac> <mrow> <mo>(</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>)</mo> </mrow> </mrow>
<mrow> <msub> <mi>g</mi> <mn>5</mn> </msub> <mo>=</mo> <mfrac> <mn>1</mn> <mn>4</mn> </mfrac> <mrow> <mo>(</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>1</mn> </msub> </mrow> <mo>)</mo> <mo>)</mo> </mrow> </mrow>
<mrow> <msub> <mi>g</mi> <mn>6</mn> </msub> <mo>=</mo> <mfrac> <mn>1</mn> <mn>4</mn> </mfrac> <mrow> <mo>(</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>+</mo> <mi>f</mi> <mo>(</mo> <mrow> <msub> <mi>T</mi> <mn>2</mn> </msub> <mo>,</mo> <msub> <mi>RH</mi> <mn>1</mn> </msub> <mo>,</mo> <msub> <mi>P</mi> <mn>2</mn> </msub> </mrow> <mo>)</mo> <mo>)</mo> </mrow> </mrow>
Wherein, f0For the analysis result, f (T, RH, P) is the experiment sample database, P0For the ambient pressure value, T0 For the ambient temperature value, RH0For the envionmental humidity, T1And T2For in the experiment sample database with T0Adjacent Two experimental temperature values, laFor T1And T2Between threshold length, l1And l2Respectively T0Correspond to T1And T2Distance;RH1With RH2For in the experiment sample database with RH0Two adjacent experiment rh values, lbFor RH1And RH2Between threshold value it is long Degree, l3And l4Respectively RH0Correspond to RH1And RH2Distance;P1And P2For in the experiment sample database with P0Adjacent two Individual experiment atmospheric pressure value, lcFor P1And P2Between threshold length, l5And l6Respectively P0Correspond to P1And P2Distance.
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CN108511084A (en) * 2018-04-02 2018-09-07 张良 A kind of method and system of intestinal flora monitoring management

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CN105572110A (en) * 2014-11-04 2016-05-11 三星电子株式会社 A method of and an apparatus for measuring biometric information

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CN105572110A (en) * 2014-11-04 2016-05-11 三星电子株式会社 A method of and an apparatus for measuring biometric information

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108511084A (en) * 2018-04-02 2018-09-07 张良 A kind of method and system of intestinal flora monitoring management

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