CN107242428A - A kind of preparation method of mangrove bark bacteriostatic agent - Google Patents
A kind of preparation method of mangrove bark bacteriostatic agent Download PDFInfo
- Publication number
- CN107242428A CN107242428A CN201710447429.9A CN201710447429A CN107242428A CN 107242428 A CN107242428 A CN 107242428A CN 201710447429 A CN201710447429 A CN 201710447429A CN 107242428 A CN107242428 A CN 107242428A
- Authority
- CN
- China
- Prior art keywords
- mangrove bark
- bacteriostatic agent
- ethanol
- preparation
- degreasing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 240000002044 Rhizophora apiculata Species 0.000 title claims abstract description 71
- 239000000022 bacteriostatic agent Substances 0.000 title claims abstract description 57
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 102
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 48
- 238000005238 degreasing Methods 0.000 claims abstract description 28
- 239000012153 distilled water Substances 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims description 29
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 20
- 235000004936 Bromus mango Nutrition 0.000 claims description 19
- 235000014826 Mangifera indica Nutrition 0.000 claims description 19
- 235000009184 Spondias indica Nutrition 0.000 claims description 19
- 238000000605 extraction Methods 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 17
- 238000000746 purification Methods 0.000 claims description 17
- 239000006166 lysate Substances 0.000 claims description 13
- 240000003793 Rhizophora mangle Species 0.000 claims description 12
- 238000005119 centrifugation Methods 0.000 claims description 12
- 239000003208 petroleum Substances 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 230000010355 oscillation Effects 0.000 claims description 7
- 241000607479 Yersinia pestis Species 0.000 claims description 4
- 239000002131 composite material Substances 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 238000002481 ethanol extraction Methods 0.000 claims description 3
- 239000013049 sediment Substances 0.000 claims description 3
- 238000004821 distillation Methods 0.000 claims description 2
- 240000007228 Mangifera indica Species 0.000 claims 1
- 238000003809 water extraction Methods 0.000 claims 1
- 235000013305 food Nutrition 0.000 abstract description 8
- 239000002994 raw material Substances 0.000 abstract description 7
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 3
- 230000003000 nontoxic effect Effects 0.000 abstract description 3
- 230000002829 reductive effect Effects 0.000 abstract description 3
- 241001093152 Mangifera Species 0.000 description 18
- 241000894006 Bacteria Species 0.000 description 15
- 241000222122 Candida albicans Species 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 6
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 241000191967 Staphylococcus aureus Species 0.000 description 6
- 229940095731 candida albicans Drugs 0.000 description 6
- 239000003755 preservative agent Substances 0.000 description 5
- 230000002335 preservative effect Effects 0.000 description 5
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 4
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 4
- 230000001408 fungistatic effect Effects 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- AEDDIBAIWPIIBD-ZJKJAXBQSA-N mangiferin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=C(OC=2C(=CC(O)=C(O)C=2)C2=O)C2=C1O AEDDIBAIWPIIBD-ZJKJAXBQSA-N 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 241001478240 Coccus Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 150000002170 ethers Chemical class 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 239000006916 nutrient agar Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 210000003491 skin Anatomy 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 239000005711 Benzoic acid Substances 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- YWQSXCGKJDUYTL-UHFFFAOYSA-N Mangiferin Natural products CC(CCC=C(C)C)C1CC(C)C2C3CCC4C(C)(C)CCCC45CC35CCC12C YWQSXCGKJDUYTL-UHFFFAOYSA-N 0.000 description 2
- 241001052560 Thallis Species 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 235000010233 benzoic acid Nutrition 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 235000019249 food preservative Nutrition 0.000 description 2
- 239000005452 food preservative Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 229940043357 mangiferin Drugs 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000000452 restraining effect Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 1
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 235000008452 baby food Nutrition 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- NWFNSTOSIVLCJA-UHFFFAOYSA-L copper;diacetate;hydrate Chemical compound O.[Cu+2].CC([O-])=O.CC([O-])=O NWFNSTOSIVLCJA-UHFFFAOYSA-L 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 235000010241 potassium sorbate Nutrition 0.000 description 1
- 239000004302 potassium sorbate Substances 0.000 description 1
- 229940069338 potassium sorbate Drugs 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 235000010199 sorbic acid Nutrition 0.000 description 1
- 239000004334 sorbic acid Substances 0.000 description 1
- 229940075582 sorbic acid Drugs 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/22—Anacardiaceae (Sumac family), e.g. smoketree, sumac or poison oak
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Alternative & Traditional Medicine (AREA)
- Birds (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Medical Informatics (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Dermatology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of preparation method of mangrove bark bacteriostatic agent, belong to bacteriostatic agent preparing technical field.The preparation method of this mangrove bark bacteriostatic agent comprises the following steps：(1) mangrove bark is handled；(2) degreasing；(3) ethanol is extracted；(4) distilled water is purified；(5) ethanol dissolves.The mangrove bark that the preparation method of this mangrove bark bacteriostatic agent well can abandon people carries out twice laid, had both reduced the discharge of rubbish, and can beautify the environment again；Raw material needed for the mangrove bark bacteriostatic agent being made has the characteristics of bactericidal effect is good, and preparation are few, and preparation method is simple, and cost is low, safety non-toxic, is a kind of new plant bacteriostatic agent.The bacteriostatic agent of the present invention is particularly suitable for use in food, while being also applied for medicine and commodity.
Description
Technical field
The present invention relates to the preparing technical field of bacteriostatic agent, and in particular to a kind of preparation method of mangrove bark bacteriostatic agent.
Background technology
Mango is mostly used in except eating raw and is processed into dried mango, mango wine, mango vinegar, canned mango etc..By fresh mango
The not shelf-stable problem of mango can be solved by being processed into all kinds of mango products, but also generate substantial amounts of mangrove bark simultaneously.At present,
The processing mode of mangrove bark, which is generally, to be abandoned, and had so not only been caused the waste of raw material but also had been polluted environment.Contain class in mangrove bark
The native chemical composition such as flavones, mangiferin, all has obvious inhibition for many microorganisms.It is estimated that mangrove bark is accounted for
The 7%~24% of fresh fruit weight, if these mangrove bark discarded objects can be used, will have to increase mango added value of product
Immeasurable effect, while a kind of new approaches can be provided for exploitation novel plant bacteriostatic agent.
Bacteriostatic agent is exactly the material of energy bacteria growing inhibiting.Bacteriostatic agent is mainly the growth and breeding process by suppressing bacterium
Needed for the synthesis of material reach the purpose of bacteria growing inhibiting breeding, bacteriostatic agent possibly can not kill bacterium, but it can be with
Suppress the growth of bacterium, prevent bacteria breed excessive and be detrimental to health.At present, it would generally be added in food production
Preservative, preservative is used to suppress microbial reproduction in food, and prevent food spoilage goes bad, during preservation to extend food
Between.But, food preservative is also one " double-edged sword ", it is possible to which the health care belt given people carrys out certain harm.In China, mesh
The preservative overwhelming majority used in preceding food production is artificial synthesized, and improper use has certain side effect；Some
Preservative is even containing micro toxin, and long-term Excess free enthalpy can cause certain infringement to health.To widely use at present
Food preservative benzoic acid exemplified by, be used for there is dispute always in the world.Such as, because existing benzoic acid and sodium benzoate
Accumulate the report of product poisoning, protection of the child group of the European Community thinks that it should not be used in infant foods, Japan also does to its use
Strict limitation is gone out.Even as one of safe preservative generally acknowledged in the world sorbic acid and potassium sorbate, Excess free enthalpy
The balance of human metabolism can be influenceed.
The content of the invention
The invention aims to the above mentioned problem for solving prior art presence, there is provided a kind of system of mangrove bark bacteriostatic agent
Preparation Method, the mangrove bark that this method well can abandon people carries out twice laid, has both reduced the discharge of rubbish, and energy
Beautify the environment；While the bacteriostasis of the native chemical compositions such as flavonoids, the mangiferin in mangrove bark can be given full play to, it is made
Raw material needed for mangrove bark bacteriostatic agent has the characteristics of bactericidal effect is good, and preparation are few, and preparation method is simple, and cost is low,
Safety non-toxic, is a kind of new plant bacteriostatic agent.The bacteriostatic agent of the present invention is particularly suitable for use in food, while being also applied for medicine
And commodity.
To reach above-mentioned purpose, the technical solution adopted by the present invention is as follows：
A kind of preparation method of mangrove bark bacteriostatic agent, comprises the following steps：
(1) mangrove bark is handled
No disease and pests harm, smooth-skined mango are selected, cleans and dries, the mangrove bark that thickness is 1~2mm is made, then dries
It is dry, crush, obtain mangrove bark powder；
(2) degreasing
Mangrove bark powder made from taking step (1), pours into petroleum ether to powder is totally submerged, stands after 20-30min, surpass
Sonication 5-10min, completes degreasing, and above-mentioned degreasing method degreasing is pressed twice with petroleum ether, outwells upper solution, takes out precipitation
Thing is dried, and obtains the mangrove bark powder after degreasing；
(3) ethanol is extracted
Mangrove bark powder after extracting degreasing, it is 85% ethanol to powder is totally submerged to pour into volume fraction, stands 15-
After 25min, supersonic oscillations 5-10min is poured out and supernatant is taken after upper solution, centrifugation, as Centrifugical extraction liquid, completes ethanol
Extraction, is extracted three times as stated above with volume fraction for 85% ethanol, and it is equal to collect the Centrifugical extraction liquid mixing of three times
It is even, obtain alcohol extraction liquid；
(4) distilled water is purified
Alcohol extraction liquid made from step (3) is taken, the distilled water for adding 3-4 times of volume is well mixed, and stands 10min, from
After heart separation, fetch water insoluble composition material, as water purification；
(5) ethanol dissolves
Take water purification made from step (4), it is that 85% ethanol is dissolved to add volume fraction, wherein water purification and
The mass ratio of 85% ethanol is 1：5-6, water purification obtains ethanol lysate after being uniformly dissolved, add and steam in ethanol lysate
Distilled water, the wherein volume ratio of ethanol lysate and distilled water are 1：35-40, mangrove bark bacteriostatic agent is can obtain after being well mixed.
Above-mentioned technical proposal, preferably, in step (1), the drying temperature is 55-65 DEG C, and drying time is 22-
25h。
Above-mentioned technical proposal, preferably, in step (1), mangrove bark is crushed to 40 mesh.
Above-mentioned technical proposal, preferably, in step (2), the time of repose is 25min, and the supersonic oscillations time is
8min。
Above-mentioned technical proposal, preferably, in step (2) and step (3), the power of the ultrasonic wave is 50%, temperature
For 20 DEG C, water level is 80.
Above-mentioned technical proposal, preferably, in step (2), the specification of the petroleum ether is 60~90 DEG C.
Above-mentioned technical proposal, preferably, in step (2), the mass ratio of mangrove bark powder and petroleum ether is 1：2.
Above-mentioned technical proposal, preferably, in step (3), the mass ratio of mangrove bark powder and 85% ethanol after degreasing
For 1：2.
Above-mentioned technical proposal, preferably, in step (3), the time of repose is 20min, and the supersonic oscillations time is
8min。
Above-mentioned technical proposal, preferably, in step (3), during the centrifugation, the rotating speed of centrifuge is 2000rpm, centrifugation
Time is 10min.
Above-mentioned technical proposal, preferably, in step (5), the mass ratio of the water purification and 85% ethanol is 1：5,
The volume ratio of the ethanol lysate and distilled water is 1：37.
Beneficial effect：
1st, the preparation method of this mangrove bark bacteriostatic agent can carry out twice laid very well, and the mangrove bark that people abandon is utilized
Come, and give full play to its use, both reduced the discharge of rubbish, moreover it is possible to beautify the environment.
2nd, the raw material made are mangrove bark discarded object, and preparation process is simple, easy to operate, so cost is low, it is easy to big
Batch production.
3rd, existing plant bacteriostatic agent is generally divided to two kinds, and a kind of is the bacteriostatic agent using various plants as raw material, and this kind antibacterial
Agent preparation process can increase cost of material and preparation technology cost；Another is the bacteriostatic agent using single plant as raw material, should
Plant bacteriostatic agent general only preferable to the fungistatic effect of certain class harmful bacteria, but broad-spectrum antibacterial effect is weaker.And it is produced by the present invention
Mangrove bark bacteriostatic agent can suppress the bacterium such as staphylococcus aureus, pseudomonas aeruginosa, Candida albicans and saccharomyces cerevisiae, not only open up
The antimicrobial spectrum for the bacteriostatic agent that single plant is raw material is opened up, scope of restraining fungi is wide, and because the present invention is just with discarded awns
Prepared by pericarp, can significantly reduce cost of material and process costs.
4th, this method adds distilled water purification and ethanol dissolving, these step energy on the basis of conventional alcohol extraction process
The purity of mangrove bark bacteriostatic agent is improved, antibacterial active ingredient can be significantly improved, the suppression of mangrove bark bacteriostatic agent made from this method
Bacterium effect is good, and safety non-toxic is environment-friendly, using extensive, and usage amount is small, available in food, medicine and commodity.
Embodiment
With reference to specific embodiment, the invention will be further described.
Embodiment 1
A kind of preparation method of mangrove bark bacteriostatic agent, comprises the following steps：
(1) sample treatment
The mango that 10 jin of no disease and pests harms, epidermis are green and smoother is bought from market, the mango is Baise of Guangxi
The beautiful awns mango of Youjiang District.Mango is cleaned up, air-dries or dries, the mangrove bark that thickness is 1~2mm is cut with fruit knife, so
Fresh mangrove bark is placed in dryer afterwards, 60 DEG C of temperature regulating, is crushed after 24h drying, be crushed to 40 mesh, mango is made
Skin powder.
(2) degreasing
Mangrove bark powder 15g is placed in conical flask, the petroleum ether that 30g specifications are 60~90 DEG C is subsequently poured into, makes oil
Ether is totally submerged mangrove bark powder, stands after 25min, ultrasonic wave (power 50%, 20 DEG C of temperature, water level 80) vibration 8min, takes off
Fat is completed, and with 60~90 DEG C of petroleum ethers, degreasing twice, outwells upper solution repeatedly as stated above, takes out sediment drying,
Obtain the mangrove bark powder after degreasing.
(3) ethanol is extracted
Mangrove bark powder 10g after extracting degreasing, pours into 20g volume parts for 85% ethanol, is totally submerged 85% ethanol
Mangrove bark powder after degreasing, stands and waits after 20min, ultrasonic wave (power 50%, 20 DEG C of temperature, water level 80) vibration 8min,
Pour out and centrifuged after upper solution, 2000rpm centrifugation 10min take supernatant, as Centrifugical extraction liquid, complete second after centrifugation
Alcohol is extracted, and is extracted three times repeatedly as stated above for 85% ethanol with volume fraction, and collect this Centrifugical extraction of three times
Liquid is well mixed, and obtains alcohol extraction liquid.
(4) distilled water is purified
Alcohol extraction liquid 10mL made from step (3) is taken, the distilled water for adding 30mL is well mixed, stands 10min,
After centrifugation, fetch water insoluble composition material, as water purification.
(5) ethanol dissolves
Water purification 2g made from step (4) is taken, it is that 85% ethanol is dissolved to add 10g volume fractions, water purification
Ethanol lysate is obtained after being uniformly dissolved, 440mL distilled water is added in ethanol lysate, mango is can obtain after being well mixed
Skin bacteriostatic agent.The storage temperature of mangrove bark bacteriostatic agent obtained by preparation is 4 DEG C.
The volume parts are the collocation method of 85% ethanol：150mL distilled water is taken to inject in 850mL AR level ethanol
Stir.
Embodiment 2
A kind of preparation method of mangrove bark bacteriostatic agent, comprises the following steps：
(1) mangrove bark is handled
No disease and pests harm, smooth-skined mango are selected, cleans and dries, the mangrove bark that thickness is 1~2mm is made, then dries
Dry, drying temperature is 65 DEG C, and drying time is 22h, is crushed to 40 mesh, obtains mangrove bark powder.
(2) degreasing
Mangrove bark powder made from taking step (1) is placed in container, then pours into 60~90 DEG C of petroleum ethers to being totally submerged awns
Pericarp powder, stands after 30min, ultrasonic wave (power 50%, 20 DEG C of temperature, water level 80) vibration 5min, completes degreasing, with 60~
90 DEG C of petroleum ethers twice, outwell upper solution by above-mentioned degreasing method degreasing, take out sediment drying, obtain the mango after degreasing
Skin powder；
(3) ethanol is extracted
Mangrove bark powder after extracting degreasing is placed in container, then it is 85% ethanol to being totally submerged degreasing to pour into volume fraction
Mangrove bark powder afterwards, stands after 15min, ultrasonic wave (power 50%, 20 DEG C of temperature, water level 80) vibration 10min, pours out upper strata
Supernatant, as Centrifugical extraction liquid are taken after solution, centrifugation, ethanol extraction is completed, three are extracted for 85% ethanol with volume fraction
It is secondary, and collect three times Centrifugical extraction liquid be well mixed, obtain alcohol extraction liquid；
(4) distilled water is purified
Alcohol extraction liquid made from step (3) is taken, the distilled water for adding 4 times of volumes is well mixed, stand 10min, centrifugation
After separation, fetch water insoluble composition material, as water purification；
(5) ethanol dissolves
Take water purification made from step (4), it is that 85% ethanol is dissolved to add volume fraction, wherein water purification and
The mass ratio of 85% ethanol is 1：6, water purification obtains ethanol lysate after being uniformly dissolved, and distillation is added in ethanol lysate
Water, the wherein volume ratio of ethanol lysate and distilled water are 1：35, it can obtain mangrove bark bacteriostatic agent after being well mixed.
The mangrove bark bacteriostatic agent prepared with embodiment 1 carries out bacteriostatic test, specific as follows：
(1) prepared by sample liquid
Culture dish, pipette tips, 0.22 μm of filter membrane, syringe and oese are sterilized into (121 DEG C, 30min).Aseptic inoculation room
Sterilized with superclean bench under uviol lamp 30min.After sterilizing is finished, by awns made from embodiment 1 in aseptic inoculation room
After the vibration of pericarp bacteriostatic agent shakes up, 25mL mangrove bark bacteriostatic agent is respectively drawn with syringe, (has been sterilized) with 0.22 μm of filter membrane
Filtering is placed in standby in conical flask.(note：With aseptically operation)
(2) prepared by culture plate
The mangrove bark bacteriostatic agent after 1mL filterings is respectively drawn with liquid-transfering gun to be placed in four culture dishes, then wherein three
The nutrient agar that volume is about 14mL is poured into culture dish, it is about 14mL's to pour into volume in another culture dish
PDA Nutrient mediums, shake up while hot, are statically placed in superclean bench and cool down.(note：Aseptically operate, culture medium must be
Less than 60 DEG C are poured into, and culture medium can not be overheated, and otherwise can destroy the active ingredient inside mangrove bark bacteriostatic agent.)
Medium component：(beef extract 3.0g, peptone 10.0g, Nacl 5.0g, agar 20g steam nutrient agar
Distilled water 1000mL, PH 7.4~7.6)；PDA culture medium (potato 200g, glucose 20g, agar 20g, water 1000mL, it is natural
pH)。
(3) it is inoculated with and cultivates
Respectively taken with liquid-transfering gun 0.1mL staphylococcus aureuses, Candida albicans, pseudomonas aeruginosa bacterium solution be inoculated in containing
On the nutrient agar of mangrove bark bacteriostatic agent, S. cervisiae bacterium solution is inoculated in the PDA nutrition containing mangrove bark bacteriostatic agent
On base, smoothened with spreading rod.Label is posted, date, strain name is finished writing.Staphylococcus aureus, Candida albicans, verdigris are false single
Born of the same parents bacterium is inverted in 37 ± 1 DEG C of constant incubators and cultivated, and S. cervisiae is inverted in 27 ± 1 DEG C of constant incubators and cultivated.
(note：Every kind of strain is inoculated with three flat boards, forms parallel control, and the culture medium after being inoculated with is entangled with freshness protection package, it is to avoid miscellaneous bacteria
Infection.)
This bacteriostatic test is additionally provided with control group, and control group does not add mangrove bark bacteriostatic agent, other conditions and embodiment 1
Experiment it is consistent.
By above-mentioned experiment is observed and recorded, mangrove bark bacteriostatic agent made from embodiment 1 is to four kinds of experimental bacterias
Influence, specific data are shown in Table 1.
Table 1
Note："-" indicates no thalli growth；"+" indicates thalli growth." A " represents staphylococcus aureus；" B " is represented
Pseudomonas aeruginosa；" C " represents Candida albicans；" D " represents saccharomyces cerevisiae.
As shown in Table 1, mangrove bark bacteriostatic agent made from embodiment 1 is notable to the fungistatic effect of saccharomyces cerevisiae, for other
Three kinds of experimental bacterias, without bacterium colony, it is small and gather to start within second day staphylococcus aureus within first day, pseudomonas aeruginosa and white
Color reads coccus and the trend grown occurs；Start staphylococcus aureus within 3rd day and pseudomonas aeruginosa growing way is slack-off, and it is white
Read coccus small and close；4th day and the 5th day Candida albicans growing way it is slack-off.Summary, mangrove bark bacteriostatic agent is to golden yellow Portugal
Grape coccus, pseudomonas aeruginosa, Candida albicans and saccharomyces cerevisiae have preferable fungistatic effect, and scope of restraining fungi is wide, fungistatic effect
It is good.
Although the present invention is disclosed as above with preferred embodiment, so it is not intended to limiting the invention, any this area skill
Art personnel, without departing from the spirit and scope of the present invention, when a little modification can be made and perfect, therefore the protection model of the present invention
Enclose when by being defined that claims are defined.
Claims (10)
1. a kind of preparation method of mangrove bark bacteriostatic agent, it is characterised in that methods described comprises the following steps：
(1) mangrove bark is handled
No disease and pests harm, smooth-skined mango are selected, cleans and dries, the mangrove bark that thickness is 1~2mm is made, then dries, powder
It is broken, obtain mangrove bark powder；
(2) degreasing
Mangrove bark powder made from taking step (1) is placed in container, then pours into petroleum ether to mangrove bark powder is totally submerged, and is stood
After 20-30min, supersonic oscillations 5-10min completes degreasing, presses above-mentioned degreasing method degreasing twice with petroleum ether, outwells upper strata
Solution, takes out sediment drying, obtains the mangrove bark powder after degreasing；
(3) ethanol is extracted
Mangrove bark powder after extracting degreasing is placed in container, then it is 85% ethanol to being totally submerged after degreasing to pour into volume fraction
Mangrove bark powder, stands after 15-25min, supersonic oscillations 5-10min takes supernatant after pouring out upper solution, centrifugation, is
Centrifugical extraction liquid, completes ethanol extraction, is extracted three times for 85% ethanol with volume fraction, and collect the Centrifugical extraction of three times
Liquid is well mixed, and obtains alcohol extraction liquid；
(4) distilled water is purified
Alcohol extraction liquid made from step (3) is taken, the distilled water for adding 3-4 times of volume is well mixed, stand 10min, centrifugation point
From rear, fetch water insoluble composition material, as water purification；
(5) ethanol dissolves
Water purification made from step (4) is taken, it is that 85% ethanol is dissolved to add volume fraction, wherein water purification and 85%
The mass ratio of ethanol is 1：5-6, water purification obtains ethanol lysate after being uniformly dissolved, distillation is added in ethanol lysate
Water, the wherein volume ratio of ethanol lysate and distilled water are 1：35-40, mangrove bark bacteriostatic agent is can obtain after being well mixed.
2. the preparation method of mangrove bark bacteriostatic agent according to claim 1, it is characterised in that：In step (1), the drying temperature
Spend for 55-65 DEG C, drying time is 22-25h.
3. the preparation method of mangrove bark bacteriostatic agent according to claim 1, it is characterised in that：In step (1), mangrove bark is crushed
To 40 mesh.
4. the preparation method of mangrove bark bacteriostatic agent according to claim 1, it is characterised in that：In step (2), during the standing
Between be 25min, the supersonic oscillations time be 8min.
5. the preparation method of mangrove bark bacteriostatic agent according to claim 1, it is characterised in that：In step (2) and step (3),
The power of the ultrasonic wave is 50%, and temperature is 20 DEG C, and water level is 80.
6. the preparation method of mangrove bark bacteriostatic agent according to claim 1, it is characterised in that：In step (2), the petroleum ether
Specification be 60~90 DEG C, the mass ratio of mangrove bark powder and petroleum ether is 1：2.
7. the preparation method of mangrove bark bacteriostatic agent according to claim 1, it is characterised in that：In step (3), the awns after degreasing
The mass ratio of pericarp powder and 85% ethanol is 1：2.
8. the preparation method of mangrove bark bacteriostatic agent according to claim 1, it is characterised in that：In step (3), during the standing
Between be 20min, the supersonic oscillations time be 8min.
9. the preparation method of mangrove bark bacteriostatic agent according to claim 1, it is characterised in that：In step (3), the centrifugation
When, the rotating speed of centrifuge is 2000rpm, and centrifugation time is 10min.
10. the preparation method of mangrove bark bacteriostatic agent according to claim 1, it is characterised in that：In step (5), the water extraction
The mass ratio of pure thing and 85% ethanol is 1：5, the volume ratio of the ethanol lysate and distilled water is 1：37.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710447429.9A CN107242428B (en) | 2017-06-14 | 2017-06-14 | Preparation method of mango peel bacteriostatic agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710447429.9A CN107242428B (en) | 2017-06-14 | 2017-06-14 | Preparation method of mango peel bacteriostatic agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107242428A true CN107242428A (en) | 2017-10-13 |
| CN107242428B CN107242428B (en) | 2020-06-30 |
Family
ID=60019320
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710447429.9A Active CN107242428B (en) | 2017-06-14 | 2017-06-14 | Preparation method of mango peel bacteriostatic agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107242428B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1844133A (en) * | 2006-04-18 | 2006-10-11 | 广西中医学院 | Process for preparing high purity mangiferin |
| CN101671330A (en) * | 2009-10-13 | 2010-03-17 | 刘全胜 | Preparation method of mangiferin |
| CN102617669A (en) * | 2012-03-03 | 2012-08-01 | 浙江大学 | Method for separating and purifying mangiferin from mango pericarp |
| CN105920065A (en) * | 2016-04-21 | 2016-09-07 | 肇庆医学高等专科学校 | Separation and purification method of gynura bicolor saponin and application thereof |
-
2017
- 2017-06-14 CN CN201710447429.9A patent/CN107242428B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1844133A (en) * | 2006-04-18 | 2006-10-11 | 广西中医学院 | Process for preparing high purity mangiferin |
| CN101671330A (en) * | 2009-10-13 | 2010-03-17 | 刘全胜 | Preparation method of mangiferin |
| CN102617669A (en) * | 2012-03-03 | 2012-08-01 | 浙江大学 | Method for separating and purifying mangiferin from mango pericarp |
| CN105920065A (en) * | 2016-04-21 | 2016-09-07 | 肇庆医学高等专科学校 | Separation and purification method of gynura bicolor saponin and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107242428B (en) | 2020-06-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101054041B1 (en) | The composition and method for large processing of fermentation extract and material fermentation which uses the dendropanax morbifera | |
| CN102783633B (en) | Method for preparing solid fermentation powder of Antrodia camphorata | |
| CN102503645B (en) | Nutrient medium for planting ganoderma lucidum | |
| CN104798824A (en) | Botanical fungicide as well as preparation, use and storage methods thereof | |
| CN101366412B (en) | Natural fruit and vegetables disinfection antistaling agent, preparation and process of using | |
| CN107509805A (en) | A kind of natural Actinidia chinensis antistaling and antiseptic agent | |
| CN107927149A (en) | A kind of application of the p-Coumaric Acid ester in sterilizing anti-staling agent for fruit and vegetable and the sterilizing anti-staling agent for fruit and vegetable of the ester containing p-Coumaric Acid | |
| CN106636214A (en) | Ginkgo biloba exocarp fermentation liquid as well as preparation method and application thereof | |
| CN103931676A (en) | Application of Chinese toon extract | |
| CN103664326A (en) | Pleurotus nebrodensis cultivation medium imitating wild cultivation and preparation method thereof | |
| CN107711834A (en) | One kind contains eugenol, cinnamic acid and citral natural built-up bactericide | |
| CN103053626B (en) | Natural lemon disinfectant and preparation method thereof | |
| CN109007006A (en) | Fruit freezing and fresh-keeping method | |
| CN105053169B (en) | A kind of edibility strawberry compound antistaling agent and preparation method thereof | |
| CN104488977A (en) | Chinese ash bark and fruit extractive and novel application thereof | |
| CN109006995A (en) | A kind of dedicated preservation agent for storing of fertile mandarin orange and preparation method thereof | |
| CN104585309B (en) | A kind of fructus corni extract natural fruit and vegetables antistaling disinfectant and its application method | |
| CN101543242B (en) | Application of sasanqua extract in antisepsis and preservation | |
| CN103843880B (en) | Based on the biological preservation liquid and uses thereof of GABA in conjunction with bio-control yeast | |
| CN107242428A (en) | A kind of preparation method of mangrove bark bacteriostatic agent | |
| CN109997911A (en) | A kind of volatility grain mold proof sustained release tablets and preparation method thereof | |
| CN110169422A (en) | A kind of combined dosage form bactericide and its preparation method and application | |
| CN109548864A (en) | A kind of preparation method of slow-release sterilizing anti-staling agent for fruit and vegetable | |
| CN109350570A (en) | A kind of cosmetics preservative and its application based on natural plant composition | |
| CN104782750B (en) | A kind of the natural anticorrosion fresh-keeping method and its antistaling agent of Mango Fruit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20171013 Assignee: Guangxi Tianyang Yuehe Agricultural Technology Service Co.,Ltd. Assignor: BAISE University Contract record no.: X2023980045589 Denomination of invention: Preparation method of a mango peel antibacterial agent Granted publication date: 20200630 License type: Common License Record date: 20231106 |
|
| EE01 | Entry into force of recordation of patent licensing contract | ||
| OL01 | Intention to license declared |