CN107242428B - Preparation method of mango peel bacteriostatic agent - Google Patents
Preparation method of mango peel bacteriostatic agent Download PDFInfo
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- CN107242428B CN107242428B CN201710447429.9A CN201710447429A CN107242428B CN 107242428 B CN107242428 B CN 107242428B CN 201710447429 A CN201710447429 A CN 201710447429A CN 107242428 B CN107242428 B CN 107242428B
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- bacteriostatic agent
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- 235000004936 Bromus mango Nutrition 0.000 title claims abstract description 117
- 235000014826 Mangifera indica Nutrition 0.000 title claims abstract description 117
- 235000009184 Spondias indica Nutrition 0.000 title claims abstract description 117
- 239000000022 bacteriostatic agent Substances 0.000 title claims abstract description 53
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 240000007228 Mangifera indica Species 0.000 title 1
- 241001093152 Mangifera Species 0.000 claims abstract description 116
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 85
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 48
- 239000012153 distilled water Substances 0.000 claims abstract description 21
- 238000005238 degreasing Methods 0.000 claims abstract description 16
- 241000196324 Embryophyta Species 0.000 claims abstract description 10
- 239000000843 powder Substances 0.000 claims description 28
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 26
- 239000000284 extract Substances 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 16
- 239000003208 petroleum Substances 0.000 claims description 13
- 238000001035 drying Methods 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 12
- 239000000469 ethanolic extract Substances 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 9
- 238000002481 ethanol extraction Methods 0.000 claims description 8
- 230000010355 oscillation Effects 0.000 claims description 8
- 238000000746 purification Methods 0.000 claims description 7
- 241000238631 Hexapoda Species 0.000 claims description 4
- 241000607479 Yersinia pestis Species 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 239000002244 precipitate Substances 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 3
- 239000012264 purified product Substances 0.000 claims description 2
- 239000002994 raw material Substances 0.000 abstract description 9
- 235000013305 food Nutrition 0.000 abstract description 8
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 231100000956 nontoxicity Toxicity 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 12
- 230000003385 bacteriostatic effect Effects 0.000 description 12
- 241000222122 Candida albicans Species 0.000 description 8
- 229940095731 candida albicans Drugs 0.000 description 8
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 7
- 241000191967 Staphylococcus aureus Species 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 239000003755 preservative agent Substances 0.000 description 6
- 239000002699 waste material Substances 0.000 description 6
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 4
- AEDDIBAIWPIIBD-ZJKJAXBQSA-N mangiferin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=C(OC=2C(=CC(O)=C(O)C=2)C2=O)C2=C1O AEDDIBAIWPIIBD-ZJKJAXBQSA-N 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000006916 nutrient agar Substances 0.000 description 3
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 239000005711 Benzoic acid Substances 0.000 description 2
- YWQSXCGKJDUYTL-UHFFFAOYSA-N Mangiferin Natural products CC(CCC=C(C)C)C1CC(C)C2C3CCC4C(C)(C)CCCC45CC35CCC12C YWQSXCGKJDUYTL-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 235000010233 benzoic acid Nutrition 0.000 description 2
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
- 150000002215 flavonoids Chemical class 0.000 description 2
- 235000017173 flavonoids Nutrition 0.000 description 2
- 235000019249 food preservative Nutrition 0.000 description 2
- 239000005452 food preservative Substances 0.000 description 2
- 229940043357 mangiferin Drugs 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000010241 potassium sorbate Nutrition 0.000 description 2
- 239000004302 potassium sorbate Substances 0.000 description 2
- 229940069338 potassium sorbate Drugs 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 235000010199 sorbic acid Nutrition 0.000 description 2
- 239000004334 sorbic acid Substances 0.000 description 2
- 229940075582 sorbic acid Drugs 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
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- 231100000252 nontoxic Toxicity 0.000 description 1
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- 235000019319 peptone Nutrition 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
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- 235000014101 wine Nutrition 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/22—Anacardiaceae (Sumac family), e.g. smoketree, sumac or poison oak
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
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- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
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Abstract
The invention discloses a preparation method of a mango peel bacteriostatic agent, and belongs to the technical field of bacteriostatic agent preparation. The preparation method of the mango peel bacteriostatic agent comprises the following steps: (1) processing mango peels; (2) degreasing; (3) extracting with ethanol; (4) purifying distilled water; (5) dissolving with ethanol. The preparation method of the mango peel bacteriostatic agent can well utilize the discarded mango peel of people, thereby reducing the discharge of garbage and beautifying the environment; the prepared mango peel bacteriostatic agent has the characteristic of good bactericidal effect, requires few raw materials for preparation, has simple preparation method, low cost, safety and no toxicity, and is a novel plant bacteriostatic agent. The bacteriostatic agent is especially suitable for food, medicine and daily necessities.
Description
Technical Field
The invention relates to the technical field of preparation of bacteriostats, and in particular relates to a preparation method of a mango peel bacteriostat.
Background
Except fresh mango, most of mangoes are used for processing dried mangoes, mango wines, mango vinegar, mango cans and the like. The problem of intolerance of storage of mangoes can be solved by processing fresh mangoes into various mango products, but a large amount of mango peels are generated at the same time. At present, the mango peel is generally discarded in a treatment mode, so that the waste of raw materials is caused, and the environment is polluted. The mango peel contains natural chemical components such as flavonoid, mangiferin and the like, and has obvious inhibition effect on a plurality of microorganisms. According to estimation, the mango peel accounts for 7-24% of the weight of fresh fruits, if the mango peel waste can be utilized, the mango peel waste has an immeasurable effect on increasing the added value of mango products, and a new idea can be provided for developing novel plant bacteriostatic agents.
Bacteriostatic agents are substances that inhibit the growth of bacteria. The bacteriostatic agent mainly achieves the purpose of inhibiting the growth and the reproduction of bacteria by inhibiting the synthesis of substances required in the growth and the reproduction processes of the bacteria, and the bacteriostatic agent can not kill the bacteria but can inhibit the growth of the bacteria and prevent the excessive breeding of the bacteria from harming the health of human bodies. At present, preservatives are usually added in the food production process, and are used for inhibiting the propagation of microorganisms in food and preventing the food from being rotted and deteriorated so as to prolong the storage time of the food. However, the food preservative is also a 'double-edged sword', which may bring certain harm to the health of people. In China, most of the preservatives used in the current food production are artificially synthesized, and certain side effects can be caused when the preservatives are not used properly; some preservatives even contain trace toxins, and long-term excessive intake of the preservatives can cause certain damage to human health. The use of benzoic acid, an example of the food preservative which is widely used at present, has been controversial internationally. For example, since the poisoning by benzoic acid and its sodium salt has been reported, the european union group for child protection has considered that it is not suitable for use in children's food, and japan has made strict restrictions on its use. Even though sorbic acid and potassium sorbate are internationally recognized as safe preservatives, excessive intake of sorbic acid and potassium sorbate can affect the balance of metabolism in the human body.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provides a preparation method of a mango peel bacteriostatic agent, which can well utilize the discarded mango peel of people, thereby reducing the discharge of garbage and beautifying the environment; meanwhile, the bacteriostatic action of natural chemical components such as flavonoid, mangiferin and the like in the mango peel can be fully exerted, the prepared mango peel bacteriostatic agent has the characteristic of good bactericidal effect, the raw materials required for preparation are few, the preparation method is simple, the cost is low, and the mango peel bacteriostatic agent is safe and nontoxic and is a novel plant bacteriostatic agent. The bacteriostatic agent is especially suitable for food, medicine and daily necessities.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a preparation method of a mango peel bacteriostatic agent comprises the following steps:
(1) mango peel treatment
Selecting mangoes without plant diseases and insect pests and with smooth skins, cleaning and airing the mangoes to prepare mango skins with the thickness of 1-2 mm, and then drying and crushing the mango skins to obtain mango skin powder;
(2) degreasing
Pouring petroleum ether into the mango peel powder prepared in the step (1) until the mango peel powder is completely immersed, standing for 20-30min, then carrying out ultrasonic oscillation for 5-10min to complete degreasing, degreasing twice with the petroleum ether according to the degreasing method, pouring off the upper solution, taking out the precipitate and drying to obtain degreased mango peel powder;
(3) ethanol extraction
Pouring 85% ethanol into degreased mango peel powder until the mango peel powder is completely immersed, standing for 15-25min, performing ultrasonic oscillation for 5-10min, pouring out an upper layer solution, centrifuging, taking supernatant, namely centrifugal extract liquid, completing ethanol extraction, extracting for three times by using 85% ethanol according to the method, collecting the centrifugal extract liquid for three times, and uniformly mixing to obtain ethanol extract liquid;
(4) purification of distilled water
Taking the ethanol extract obtained in the step (3), adding distilled water with the volume of 3-4 times of that of the ethanol extract, uniformly mixing, standing for 10min, and after centrifugal separation, taking water insoluble component substances to obtain a water purified product;
(5) dissolving with ethanol
And (3) taking the water extract prepared in the step (4), and adding 85% ethanol in volume fraction for dissolving, wherein the mass ratio of the water extract to the 85% ethanol is 1: 5-6, uniformly dissolving the water extract to obtain an ethanol solution, and adding distilled water into the ethanol solution, wherein the volume ratio of the ethanol solution to the distilled water is 1: 35-40, and uniformly mixing to obtain the mango peel bacteriostatic agent.
According to the technical scheme, preferably, in the step (1), the drying temperature is 55-65 ℃, and the drying time is 22-25 h.
Preferably, in the step (1), the mango peel is crushed to 40 meshes.
In the above technical solution, preferably, in the step (2), the standing time is 25min, and the ultrasonic oscillation time is 8 min.
In the above technical solution, preferably, in the step (2) and the step (3), the power of the ultrasonic wave is 50%, the temperature is 20 ℃, and the water level is 80.
In the above technical scheme, preferably, in the step (2), the specification of the petroleum ether is 60-90 ℃.
According to the technical scheme, preferably, in the step (2), the mass ratio of the mango peel powder to the petroleum ether is 1: 2.
preferably, in the step (3), the mass ratio of the defatted mango peel powder to 85% ethanol is 1: 2.
in the above technical solution, preferably, in the step (3), the standing time is 20min, and the ultrasonic oscillation time is 8 min.
Preferably, in the step (3), the rotation speed of the centrifuge is 2000rpm, and the centrifugation time is 10 min.
Preferably, in the step (5), the mass ratio of the water purification product to 85% ethanol is 1: 5, the volume ratio of the ethanol solution to the distilled water is 1: 37.
has the advantages that:
1. the preparation method of the mango peel bacteriostatic agent can well utilize wastes, utilizes the mango peel discarded by people, fully exerts the functions of the mango peel, reduces the discharge of garbage and beautifies the environment.
2. The raw material for making is mango peel waste, and the preparation process is simple, easy to operate, low in cost and easy for mass production.
3. The existing plant bacteriostatic agent is generally divided into two types, one type is bacteriostatic agent taking a plurality of plants as raw materials, and the preparation process of the bacteriostatic agent can increase the cost of the raw materials and the cost of the preparation process; the other is a bacteriostatic agent which takes single plants as raw materials, and the bacteriostatic agent generally has good bacteriostatic effect only on certain harmful bacteria, but has weak broad-spectrum bacteriostatic effect. The mango peel bacteriostatic agent prepared by the invention can inhibit staphylococcus aureus, pseudomonas aeruginosa, candida albicans, saccharomyces cerevisiae and other bacteria, not only expands the bacteriostatic spectrum of the bacteriostatic agent taking a single plant as a raw material and has a wide bacteriostatic range, but also can greatly reduce the raw material cost and the process cost because the mango peel bacteriostatic agent is prepared by only utilizing waste mango peel.
4. The method adds the distilled water purification and the ethanol dissolution on the basis of the common alcohol extraction process, and the steps can improve the purity of the mango peel bacteriostatic agent and can obviously improve the bacteriostatic effective components.
Detailed Description
The present invention is further illustrated by the following specific examples.
Example 1
A preparation method of a mango peel bacteriostatic agent comprises the following steps:
(1) sample processing
10 jin of mangoes which are free from diseases and insect pests, have green and relatively smooth skins are purchased from the market, and are Yu mango in Guangxi Bai-color Right river district. Cleaning mango, air-drying or airing, cutting mango peel with the thickness of 1-2 mm by using a fruit knife, then putting the fresh mango peel into a dryer, adjusting the temperature to 60 ℃, drying for 24 hours, then crushing, and crushing to 40 meshes to obtain mango peel powder.
(2) Degreasing
Putting 15g of mango peel powder into a conical flask, pouring 30g of petroleum ether with the specification of 60-90 ℃ to enable the petroleum ether to completely immerse the mango peel powder, standing for 25min, oscillating for 8min by using ultrasonic waves (the power is 50%, the temperature is 20 ℃, and the water level is 80), completing degreasing, repeatedly degreasing twice by using the petroleum ether with the temperature of 60-90 ℃ according to the method, pouring out the upper-layer solution, taking out the precipitate, and drying to obtain the degreased mango peel powder.
(3) Ethanol extraction
Taking 10g of degreased mango peel powder, pouring 20g of 85% ethanol in parts by volume, completely immersing the degreased mango peel powder in the 85% ethanol, standing for 20min, oscillating for 8min by using ultrasonic waves (power is 50%, temperature is 20 ℃, and water level is 80), pouring out an upper layer solution, centrifuging for 10min at 2000rpm, centrifuging, taking a supernatant, namely a centrifugal extract, completing ethanol extraction, repeatedly extracting for three times by using the ethanol with the volume fraction of 85% according to the method, collecting the centrifugal extracts of the three times, and uniformly mixing to obtain an ethanol extract.
(4) Purification of distilled water
And (4) taking 10mL of the ethanol extract prepared in the step (3), adding 30mL of distilled water, uniformly mixing, standing for 10min, and after centrifugal separation, taking water-insoluble component substances, namely the water extract.
(5) Dissolving with ethanol
And (3) taking 2g of the water extract prepared in the step (4), adding 10g of ethanol with the volume fraction of 85% for dissolving, uniformly dissolving the water extract to obtain an ethanol solution, adding 440mL of distilled water into the ethanol solution, and uniformly mixing to obtain the mango peel bacteriostatic agent. The preservation temperature of the prepared mango peel bacteriostatic agent is 4 ℃.
The preparation method of the ethanol with the volume parts of 85 percent comprises the following steps: 150mL of distilled water is poured into 850mL of AR-grade ethanol and stirred uniformly.
Example 2
A preparation method of a mango peel bacteriostatic agent comprises the following steps:
(1) mango peel treatment
Selecting mangoes without plant diseases and insect pests and with smooth skins, cleaning and airing the mangoes to prepare mango skins with the thickness of 1-2 mm, then drying the mango skins at the temperature of 65 ℃ for 22h, and crushing the mango skins to 40 meshes to obtain mango skin powder.
(2) Degreasing
Putting the mango peel powder prepared in the step (1) into a container, pouring 60-90 ℃ petroleum ether until the mango peel powder is completely immersed, standing for 30min, oscillating for 5min by ultrasonic waves (power is 50%, temperature is 20 ℃, and water level is 80) to finish degreasing, degreasing twice by the degreasing method with the 60-90 ℃ petroleum ether, pouring out an upper layer solution, taking out a precipitate, and drying to obtain degreased mango peel powder;
(3) ethanol extraction
Putting the degreased mango peel powder into a container, pouring 85% ethanol by volume fraction until the degreased mango peel powder is completely immersed, standing for 15min, oscillating for 10min by ultrasonic waves (power is 50%, temperature is 20 ℃, water level is 80), pouring out an upper layer solution, centrifuging, taking a supernatant, namely a centrifugal extract, completing ethanol extraction, extracting for three times by using 85% ethanol by volume fraction, collecting the centrifugal extracts of the three times, and uniformly mixing to obtain an ethanol extract;
(4) purification of distilled water
Taking the ethanol extract prepared in the step (3), adding distilled water with 4 times of volume, uniformly mixing, standing for 10min, and taking water insoluble component substances after centrifugal separation to obtain a water purified substance;
(5) dissolving with ethanol
And (3) taking the water extract prepared in the step (4), and adding 85% ethanol in volume fraction for dissolving, wherein the mass ratio of the water extract to the 85% ethanol is 1: 6, uniformly dissolving the water extract to obtain an ethanol solution, and adding distilled water into the ethanol solution, wherein the volume ratio of the ethanol solution to the distilled water is 1: 35, and mixing uniformly to obtain the mango peel bacteriostatic agent.
The mango peel bacteriostatic agent prepared in example 1 is used for bacteriostatic tests, and the bacteriostatic test method comprises the following specific steps:
(1) sample liquid preparation
The petri dish, the tip, the 0.22 μm filter, the syringe and the inoculating loop were sterilized (121 ℃, 30 min). Sterilizing the sterile inoculation room and the ultra-clean workbench for 30min under an ultraviolet lamp. After sterilization, the mango peel bacteriostat prepared in example 1 is shaken and evenly shaken in an aseptic inoculation room, 25mL of the mango peel bacteriostat is respectively absorbed by a syringe, filtered by a filter membrane (sterilized) with the diameter of 0.22 μm and placed in a conical flask for standby. (Note: operation under sterile conditions)
(2) Preparation of culture plate
Sucking 1mL of filtered mango peel bacteriostatic agent by using a pipette gun respectively, placing the filtered mango peel bacteriostatic agent into four culture dishes, pouring 14mL of nutrient agar culture medium into three culture dishes, pouring 14mL of PDA nutrient medium into the other culture dish, shaking up the culture dishes while the culture dishes are hot, and standing the culture dishes in a clean bench for cooling. (Note: operating under aseptic conditions, the culture medium must be poured at a temperature below 60 ℃, the culture medium cannot be overheated, otherwise the effective components in the mango peel bacteriostatic agent can be damaged.)
The components of the culture medium: a nutrient agar culture medium (3.0 g of beef extract, 10.0g of peptone, 5.0g of NaCl, 20g of agar, 1000mL of distilled water and pH 7.4-7.6); PDA medium (potato 200g, glucose 20g, agar 20g, water 1000mL, natural pH).
(3) Inoculation and culture
0.1mL of staphylococcus aureus, candida albicans and pseudomonas aeruginosa bacterial liquid is respectively inoculated on a nutrient agar culture medium containing a mango peel bacteriostatic agent by using a liquid transfer gun, and the saccharomyces cerevisiae bacterial liquid is inoculated on a PDA nutrient medium containing the mango peel bacteriostatic agent and is uniformly coated by using a coating rod. Labeling, writing date and strain name. The staphylococcus aureus, the candida albicans and the pseudomonas aeruginosa are inversely placed in a constant temperature incubator at 37 +/-1 ℃ for culture, and the saccharomyces cerevisiae is inversely placed in a constant temperature incubator at 27 +/-1 ℃ for culture. (Note: three plates were inoculated per strain to form a parallel control, and the inoculated medium was covered with a freshness-retaining bag to prevent infection with foreign bacteria.)
The bacteriostasis test is also provided with a control group, wherein no mango peel bacteriostat is added into the control group, and other conditions are consistent with the test of the embodiment 1.
Through observation and recording of the tests, the influence of the mango peel bacteriostatic agent prepared in example 1 on four experimental bacteria is shown in table 1.
TABLE 1
Note: "-" indicates no growth of the cells; "+" indicates that there is growth of bacteria. "A" represents Staphylococcus aureus; "B" represents Pseudomonas aeruginosa; "C" means Candida albicans; "D" represents Saccharomyces cerevisiae.
As can be seen from the table 1, the mango peel bacteriostatic agent prepared in example 1 has a remarkable bacteriostatic effect on saccharomyces cerevisiae, and for other three experimental bacteria, no bacterial colony exists in the first day, staphylococcus aureus is small and gathered in the second day, and pseudomonas aeruginosa and candida albicans have growth trends; on the third day, the growth vigor of staphylococcus aureus and pseudomonas aeruginosa becomes slow, and the candida albicans is small and dense; the growth of Candida albicans became slow on the fourth and fifth days. By combining the above, the mango peel bacteriostatic agent has a good bacteriostatic effect on staphylococcus aureus, pseudomonas aeruginosa, candida albicans and saccharomyces cerevisiae, and is wide in bacteriostatic range and good in bacteriostatic effect.
Although the present invention has been described with respect to the preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (8)
1. A preparation method of a mango peel bacteriostatic agent is characterized by comprising the following steps:
(1) mango peel treatment
Selecting mangoes without plant diseases and insect pests and with smooth skins, cleaning and airing the mangoes to prepare mango skins with the thickness of 1-2 mm, and then drying and crushing the mango skins to obtain mango skin powder; the drying temperature is 55-65 ℃, and the drying time is 22-25 h;
(2) degreasing
Putting the mango peel powder prepared in the step (1) into a container, pouring petroleum ether until the mango peel powder is completely immersed, standing for 20-30min, performing ultrasonic oscillation for 5-10min to complete degreasing, degreasing twice with the petroleum ether according to the degreasing method, pouring an upper layer solution, taking out a precipitate, and drying to obtain degreased mango peel powder; the specification of the petroleum ether is 60-90 ℃, and the mass ratio of the mango peel powder to the petroleum ether is 1: 2;
(3) ethanol extraction
Putting the degreased mango peel powder into a container, pouring 85% ethanol by volume fraction until the degreased mango peel powder is completely immersed, standing for 15-25min, performing ultrasonic oscillation for 5-10min, pouring out an upper-layer solution, centrifuging, taking a supernatant, namely a centrifugal extract, completing ethanol extraction, extracting for three times by using 85% ethanol by volume fraction, collecting the centrifugal extracts of the three times, and uniformly mixing to obtain an ethanol extract;
(4) purification of distilled water
Taking the ethanol extract obtained in the step (3), adding distilled water with the volume of 3-4 times of that of the ethanol extract, uniformly mixing, standing for 10min, and after centrifugal separation, taking water insoluble component substances to obtain a water purified product;
(5) dissolving with ethanol
And (3) taking the water extract prepared in the step (4), and adding 85% ethanol in volume fraction for dissolving, wherein the mass ratio of the water extract to the 85% ethanol is 1: 5-6, uniformly dissolving the water extract to obtain an ethanol solution, and adding distilled water into the ethanol solution, wherein the volume ratio of the ethanol solution to the distilled water is 1: 35-40, and uniformly mixing to obtain the mango peel bacteriostatic agent.
2. The method for preparing the mango peel bacteriostatic agent according to claim 1, which is characterized in that: in the step (1), the mango peel is crushed to 40 meshes.
3. The method for preparing the mango peel bacteriostatic agent according to claim 1, which is characterized in that: in the step (2), the standing time is 25min, and the ultrasonic oscillation time is 8 min.
4. The method for preparing the mango peel bacteriostatic agent according to claim 1, which is characterized in that: in the step (2) and the step (3), the power of the ultrasonic wave is 50%, the temperature is 20 ℃, and the water level is 80.
5. The method for preparing the mango peel bacteriostatic agent according to claim 1, which is characterized in that: in the step (3), the mass ratio of the degreased mango peel powder to 85% ethanol is 1: 2.
6. the method for preparing the mango peel bacteriostatic agent according to claim 1, which is characterized in that: in the step (3), the standing time is 20min, and the ultrasonic oscillation time is 8 min.
7. The method for preparing the mango peel bacteriostatic agent according to claim 1, which is characterized in that: in the step (3), during the centrifugation, the rotating speed of the centrifuge is 2000rpm, and the centrifugation time is 10 min.
8. The method for preparing the mango peel bacteriostatic agent according to claim 1, which is characterized in that: in the step (5), the mass ratio of the water purification product to 85% ethanol is 1: 5, the volume ratio of the ethanol solution to the distilled water is 1: 37.
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| CN1844133A (en) * | 2006-04-18 | 2006-10-11 | 广西中医学院 | Process for preparing high purity mangiferin |
| CN101671330A (en) * | 2009-10-13 | 2010-03-17 | 刘全胜 | Preparation method of mangiferin |
| CN102617669A (en) * | 2012-03-03 | 2012-08-01 | 浙江大学 | Method for separating and purifying mangiferin from mango pericarp |
| CN105920065A (en) * | 2016-04-21 | 2016-09-07 | 肇庆医学高等专科学校 | Separation and purification method of gynura bicolor saponin and application thereof |
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|---|---|---|---|---|
| CN1844133A (en) * | 2006-04-18 | 2006-10-11 | 广西中医学院 | Process for preparing high purity mangiferin |
| CN101671330A (en) * | 2009-10-13 | 2010-03-17 | 刘全胜 | Preparation method of mangiferin |
| CN102617669A (en) * | 2012-03-03 | 2012-08-01 | 浙江大学 | Method for separating and purifying mangiferin from mango pericarp |
| CN105920065A (en) * | 2016-04-21 | 2016-09-07 | 肇庆医学高等专科学校 | Separation and purification method of gynura bicolor saponin and application thereof |
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Application publication date: 20171013 Assignee: Guangxi Tianyang Yuehe Agricultural Technology Service Co.,Ltd. Assignor: BAISE University Contract record no.: X2023980045589 Denomination of invention: Preparation method of a mango peel antibacterial agent Granted publication date: 20200630 License type: Common License Record date: 20231106 |