CN107509805A - A kind of natural Actinidia chinensis antistaling and antiseptic agent - Google Patents

A kind of natural Actinidia chinensis antistaling and antiseptic agent Download PDF

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Publication number
CN107509805A
CN107509805A CN201710908615.8A CN201710908615A CN107509805A CN 107509805 A CN107509805 A CN 107509805A CN 201710908615 A CN201710908615 A CN 201710908615A CN 107509805 A CN107509805 A CN 107509805A
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CN
China
Prior art keywords
parts
chitosan
natural
antiseptic agent
actinidia chinensis
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CN201710908615.8A
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Chinese (zh)
Inventor
何明太
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Ya'an Kiwifruit Industry Technology Research Institute
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Ya'an Kiwifruit Industry Technology Research Institute
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Priority to CN201710908615.8A priority Critical patent/CN107509805A/en
Publication of CN107509805A publication Critical patent/CN107509805A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/14Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • A23B7/154Organic compounds; Microorganisms; Enzymes

Abstract

The invention discloses a kind of natural Actinidia chinensis antistaling and antiseptic agent, the natural fresh-keeping preservative constituent includes:Eugenol, forulic acid, cinnamic acid, citronellol, extract solution from aloe, qinghaosu, chitosan, propolis extract.

Description

A kind of natural Actinidia chinensis antistaling and antiseptic agent
Technical field
The present invention relates to preservative field, more particularly to a kind of natural Actinidia chinensis antistaling and antiseptic agent.
Background technology
Kiwi berry is a kind of perennial vine fruit of falling leaves, and belongs to the Actinidia of Actinidiaceae, belongs to half cloudy natural disposition and plants Thing, strong adaptability, avoid strong sunshine, originate in China Yangtze river basin, be grown on below height above sea level 2000m more, Kiwi berry is called again Carambola, Chinese gooseberry, Mao Litao.At present, in the whole world it has been found that 66 kinds of actinidia tree, wherein there is actinidia tree in China 62 kinds, it can be seen that, China possesses the Germplasm Resources in Genera Actinidia of very abundant.Before the cultivation history of Kiwi berry originates from 1200 Shaanxi.Investigated according to relevant information, before 1,000 years《It is elegant》、《The Book of Songs》In note on Kiwi berry just be present Carry;Also it is related to describe the verse of Kiwi berry in the Tang Dynasty poet Cen Can poem, i.e., " on Zhongting curb, a frame Kiwi berry ";It is bright For the medical literature of Li Shizhen (1518-1593 A.D.)《Compendium of Materia Medica》In also mention Kiwi berry, i.e., " Kiwi berry, it is shaped like pears, its color such as peach, and Mi Monkey peach eating, therefore have all names.Kiwi berry, although the history for there was only more than 100 years so far, it is 20th century wild fruit tree quilt Artificial domesticating cultivation and most have one of the four of remarkable effect big fruit tree species.1904, New Zealand introduced a fine variety macaque from China Peach, and first New Zealand's Kiwifruit Culture orchard has been built up in nineteen thirty, subsequent Kiwi berry artificial cultivation rapidly expands;20 generation Record after the seventies, Kiwi berry is commercialized cultivation technique and popularized in the whole world, and statistics is understood so far, whole world Kiwifruit Culture area 14.3 ten thousand hm2 are reached, total output is more than 1,750,000 t.At the same time, in China, Kiwi berry has been taken as a planting fruit-trees Cultivation, and channel is being introduced a fine variety from two aspects, on the one hand by introducing some improved seeds from New Zealand, on the other hand then rely on Greatly develop the fine-variety breeding work of oneself.Learnt according to relevant report, China's Kiwi berry total output has reached 250 within 1975 ~400 ten thousand kilograms;Late 1990s, 49.8 ten thousand mu of Actinidia garden is to having built up.China is the original of Kiwi berry State is produced, while is also the first big producer of world's Kiwi berry, Kiwi berry industry turns into China subregion rural area and passed through already The pillar industry of Ji development.
Kiwi berry is due to containing substantial amounts of vitamins and other nutritious components and as indispensable water in people's daily life One of fruit.Although most of fresh fruit of vegetables contents of saccharide is not high, protein content is also seldom, and fat is lower, and they are rich in more Kind vitamin, abundant inorganic salts and dietary fiber, can not only provide nutrition, can also improve a poor appetite, help digest.So Fruits and vegetables are the second key farm products for being only second to grain.Fruits and vegetables have very important economic value simultaneously, and it not only can be with fresh Pin, but also be that the main of food industry processes raw material, so as to further increase the added value of fruits and vegetables.
It is especially Kiwi berry nutritious abundant but fresh fruit and vegetable food is fresh and tender perishable, and postharvest fruit and vegetable still has life, Metabolism can be constantly carried out, nutrient and moisture is consumed, makes product freshness and quality decline.In addition because of product moisture and sugar Content it is higher, be subject to the invasion and attack of microorganism and insect, transport for long-distance and storage in easily rot, lose business Product are worth.In addition, there is fruits and vegetables obvious seasonal and territorial characteristics, its production substantially to be restricted with harvest time by season, Busy season listing number is big, and supply exceed demand, and dull season single varieties, listing number is few, and supply falls short of demand, and anniversary price fluctuation is larger.Together When, fruits and vegetables production is very big by eco-environmental impact, and the fruit and vegetable varieties of different regions suitable growth are different.Quality product has it suitable Suitable producing region, Kiwi berry are abounded with the band of the Qinling Mountains one.Same fruits and vegetables produce in different regions, its production period, water content in harvest, sell Goods amount, quality, production cost and selling price are often also different.These features largely limit the circulation of fruits and vegetables, make Its economic value substantially reduces.And postharvest storage preservation technology can reduce the loss during fruit and vegetable storage and transportation, while can also prolong Slow aging and its reduction of quality, extend fruits and vegetables shelf life, the economic value of fruits and vegetables are improved while people's demand is met.Cause This, postharvest fruit and vegetable preservation technology turns into the study hotspot in this field.
It is widely used in the range of current All Around The World, the technology of postharvest fruit and vegetable storage fresh-keeping includes physical measure and fresh-keeping Agent is handled.Wherein physical means include heat treatment, cryopreservation, air conditioned storage, subatmospheric pressure storage, electromagnetic radiation storage, ozone from Son storage etc.;Physical measure is nontoxic, and noresidue is pollution-free, and safety convenient is workable, but anti-corrosion dynamics is limited, And it is limited by the field, antisepsis antistaling agent have it is easy to use, can more fully the nutritional ingredient of retained product and flavor be special The characteristics of levying and not consuming energy, but long-term, a large amount of uses of these antisepsis antistaling agents, postharvest pathogen can be caused to produce anti-medicine Property, residual can be also produced in agricultural product and environment and is not easily decomposed, there is great potential safety hazard, pole to human body and environment The earth limits the sale of China's fruits and vegetables in the international market.With people environmental protection, health perception increasingly enhancing, high-quality, Harmless fruits and vegetables are just gradually being concerned by people and heat is held in both hands.Therefore, limit or subtract with Nuisanceless high-efficient antisepsis antistaling agent The use of few antifungal agent is imperative.
Natural source antistaling agent has safe and nontoxic, noresidue, small to environmental pressure, to the non-target life such as people, animal, natural enemy The features such as thing is harmless, therefore, the present invention have studied a kind of natural Actinidia chinensis antistaling and antiseptic agent.
The content of the invention
It is long-term, a large amount of uses present invention mainly solves the technical problem of existing antisepsis antistaling agent, can causes to adopt rear cause of disease Bacterium is developed immunity to drugs, and residual can be also produced in agricultural product and environment and is not easily decomposed, and to human body and environment, there is great Potential safety hazard provides a kind of natural antisepsis fresh-keeping agent, has safe and nontoxic, noresidue, small to environmental pressure, it is harmless the characteristics of, Changing the constituent of natural antisepsis fresh-keeping agent includes:Eugenol, forulic acid, cinnamic acid, citronellol, extract solution from aloe, qinghaosu, Chitosan, propolis extract.
Chitosan molecule has microcosmic network structure, has very strong water retention property, while also have good film forming characteristics, shape Into film there is differential permeability, it has antibiotic property simultaneously, Kiwi berry can be protected not contaminated by microorganism, chitosan coating energy Kiwi berry exterior quality and tissue morphology are kept, the Tibetan shelf life of Kiwi berry can be extended.
Preferably, the composition is calculated as by weight, 9~15 parts of eugenol, 8~14 parts of forulic acid, cinnamic acid 9~15 Part, 10~17 parts of citronellol, 7~12 parts of extract solution from aloe, 9~13 parts of qinghaosu, 9~14 parts of propolis.
In one embodiment of the invention, the composition is calculated as by weight, 12 parts of eugenol, 11 parts of forulic acid, meat 13 parts of cinnamic acid, 15 parts of citronellol, 10 parts of extract solution from aloe, 11 parts of qinghaosu, 12 parts of propolis.
Preferably, the composition chitosan degree of deacetylation is 70%~95%.
Chitosan degree of deacetylation is that 70%~95% scope chitosan anti-bacteria activity is linear with chitosan degree of acetylation Relation.
Preferably, the composition chitosan degree of deacetylation is 93.6%.
Degree of deacetylation increase means the increase of chitosan free amine group the result is that chitosan matter in acid condition The increase of sonization amino quantity, and can be dissolved completely in water so as to cause chitosan and the negatively charged cell membrane of microorganism it The chance of interphase interaction increases, and it is most effective to take off chitosan anti-bacteria activity when second phthalein degree is 93.6%.
Preferably, the chitosan is crab or shrimp pressure vessel glycan.
The antibacterial activity of its antibacterial activity fungal source chitosan of chitosan source impact is weaker than crab or shrimp shell pressure vessel glycan, because There is relatively low relative molecular mass to come crab or shrimp shell pressure vessel glycan.
Preferably, the chitosan is 19~26 parts in parts by mass.
There is dependence between chitosan anti-bacteria activity and its usage amount, the antibacterial action of chitosan is by cell intracellular component Leakage caused by.When chitosan is in parts by mass 19~26 parts, bacteria cell wall is covered by chitosan chain, chitosan With the interphase interaction increase of chitosan, it disturbs reacting to each other between chitosan and bacterium surface.
Preferably, the chitosan is 22 parts in parts by mass.
Preferably, it is food-grade that the chitosan, which is pressed,.
Preferably, the preparation method of the propolis extract includes,
A. 40 parts of ethanol solutions, 30 parts of ethyl acetate, 20 parts of glycerine, 10 part of 16% sucrose are uniformly mixed, formed Mixed solution;
B. by 20 parts of 6~8h of extraction under the conditions of 50~58 DEG C of propolis, supernatant is taken to be freeze-dried after being concentrated in vacuo, i.e., .
Contain multiple biological activities composition in propolis extract, there is extremely strong fungicidal effectiveness.Propolis extract is to a variety of doctors Learning fungi such as Mycotoruloides, Epidermophyton, Penicillium etc. has stronger inhibitory action;It is common that some can be suppressed simultaneously The growth of food pathogenic fungi such as Alternaria, Penicillium, Eurotium etc..
Natural Actinidia chinensis antistaling and antiseptic agent of the present invention has the bacteriostatic activity of wide spectrum, either Gram-negative or the positive Bacterium, or saccharomycete and mould, there is very strong fungistatic effect, therefore, have been mutually reinforcing between each component of the invention antibacterial Activity, expand scope of restraining fungi.
The each component of the present invention is natural antibacterial composition, nontoxic, harmless, effectively, protects the economic valency of Kiwi berry Value, promotes the development of Kiwi berry industry.
Embodiment
Embodiment 1
The preparation method of propolis extract:
A. 40 parts of ethanol solutions, 30 parts of ethyl acetate, 20 parts of glycerine, 10 part of 16% sucrose are uniformly mixed, formed Mixed solution;
B. by 20 parts of 6~8h of extraction under the conditions of 50~58 DEG C of propolis, supernatant is taken to be freeze-dried after being concentrated in vacuo, i.e., .
Embodiment 2
(1) 12 parts of eugenol, 11 parts of forulic acid, 20 parts of 13 parts of cinnamic acid plus ethanol are well mixed.
(2) 15 parts of citronellol is weighed, 10 parts of extract solution from aloe, 11 parts of qinghaosu, adds 10 parts of ethanol well mixed.
(3) 12 parts of propolis is weighed, 22 parts of chitosan, the mixed liquor added in step (1) and (2), 50min is stirred, produces, Natural Actinidia chinensis antistaling and antiseptic agent of the present invention.
Embodiment 3
(1) 9 parts of eugenol, 14 parts of forulic acid, 20 parts of 9 parts of cinnamic acid plus ethanol are well mixed.
(2) 17 parts of citronellol is weighed, 7 parts of extract solution from aloe, 13 parts of qinghaosu, adds 10 parts of ethanol well mixed.
(3) 9 parts of propolis is weighed, 19 parts of chitosan, the mixed liquor added in step (1) and (2), 50min is stirred, produces, Natural Actinidia chinensis antistaling and antiseptic agent of the present invention.
Embodiment 4
(1) 15 parts of eugenol, 8 parts of forulic acid, 20 parts of 15 parts of cinnamic acid plus ethanol are well mixed.
(2) 10 parts of citronellol is weighed, 12 parts of extract solution from aloe, 9 parts of qinghaosu, adds 10 parts of ethanol well mixed.
(3) 14 parts of propolis is weighed, 26 parts of chitosan, the mixed liquor added in step (1) and (2), 50min is stirred, produces, Natural Actinidia chinensis antistaling and antiseptic agent of the present invention.
Embodiment 5
By above-described embodiment 2~4 hereinafter referred to as sample 1~3 make antistaling agent be applied to harvesting after Kiwi berry it is fresh-keeping with Without using the Kiwi berry (hereinafter referred to as blank) of antistaling agent, room temperature storage surveys its freshness result after 90 days as follows
Blank Sample 1 Sample 2 Sample 3
Weight-loss ratio (%) 10.02 1.13 1.24 1.39
Decayed fruit rate (%) 30 13 16 15
Embodiment 6
(1) under aseptic technique, the ring inclined-plane culture of picking one for examination bacterium, is inoculated in corresponding fluid nutrient medium respectively In, 25~37 DEG C of constant incubator (every kind of to have corresponding temperature for examination bacterium) cultivates 24h, and sterilized physiological saline gradient is dilute Release, the concentration for adjusting bacteria suspension is 106~107cfu/mL, standby.
(2) by the agar medium heating and melting of sterilizing, pour into sterilized culture dish, flat board is made.Agar solidification After cooling, take 1ml bacteria suspensions to be placed on flat board, scrape coating uniformly with triangular glass, unnecessary bacteria suspension is carefully suctioned out, stood several Minute, drum rim is placed after to be solidified, 0.9g/L sample 1,2,3 is added in different drum rims respectively, culture dish is put In 26~37 DEG C of constant incubator, after 24h, the size of inhibition zone, straight with inhibition zone around vernier caliper measurement sample.
Result of the test is as follows:
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this hair The equivalent structure or equivalent flow conversion that bright description is made, or directly or indirectly it is used in other related technology necks Domain, it is included within the scope of the present invention.

Claims (10)

1. a kind of natural Actinidia chinensis antistaling and antiseptic agent, it is characterised in that the natural fresh-keeping preservative constituent includes:Cloves Phenol, forulic acid, cinnamic acid, citronellol, extract solution from aloe, qinghaosu, chitosan, propolis extract.
2. a kind of natural Actinidia chinensis antistaling and antiseptic agent according to claim 1, it is characterised in that the composition is by weight It is calculated as, 9 ~ 15 parts of eugenol, 8 ~ 14 parts of forulic acid, 9 ~ 15 parts of cinnamic acid, 10 ~ 17 parts of citronellol, 7 ~ 12 parts of extract solution from aloe, it is blue or green 9 ~ 13 parts of artemisin, 9 ~ 14 parts of propolis.
3. a kind of natural Actinidia chinensis antistaling and antiseptic agent according to claim 2, it is characterised in that the composition is by weight It is calculated as, 12 parts of eugenol, 11 parts of forulic acid, 13 parts of cinnamic acid, 15 parts of citronellol, 10 parts of extract solution from aloe, 11 parts of qinghaosu, honeybee 12 parts of glue.
4. a kind of natural Actinidia chinensis antistaling and antiseptic agent according to claim 1, it is characterised in that the composition chitosan takes off Degree of acetylation is 70% ~ 95%.
5. a kind of natural Actinidia chinensis antistaling and antiseptic agent according to claim 4, it is characterised in that the composition chitosan takes off Degree of acetylation is 93.6%.
A kind of 6. natural Actinidia chinensis antistaling and antiseptic agent according to claim 1, it is characterised in that the chitosan be crab or Shrimp pressure vessel glycan.
7. device according to claim 1, it is characterised in that the chitosan is 19 ~ 26 parts in parts by mass.
8. device according to claim 7, it is characterised in that the chitosan is 22 parts in parts by mass.
9. device according to claim 1, it is characterised in that it is food-grade that the chitosan, which is pressed,.
10. device according to claim 1, it is characterised in that the preparation method of the propolis extract includes, and a. will 40 parts of ethanol solutions, 30 parts of ethyl acetate, 20 parts of glycerine, 10 part of 16% sucrose are uniformly mixed, and form mixed solution;
B. by 20 parts of 6 ~ 8h of extraction under the conditions of 50 ~ 58 DEG C of propolis, take supernatant to be freeze-dried after being concentrated in vacuo, produce.
CN201710908615.8A 2017-09-29 2017-09-29 A kind of natural Actinidia chinensis antistaling and antiseptic agent Pending CN107509805A (en)

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CN108576556A (en) * 2018-03-27 2018-09-28 浙江工商大学 A kind of preserving antistaling agent composition formula of the multiple own ester containing ferulic acid and application
CN109042848A (en) * 2018-07-25 2018-12-21 广西田阳县创新农业综合开发有限公司 A kind of mango preservative and preparation method thereof

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108576556A (en) * 2018-03-27 2018-09-28 浙江工商大学 A kind of preserving antistaling agent composition formula of the multiple own ester containing ferulic acid and application
CN109042848A (en) * 2018-07-25 2018-12-21 广西田阳县创新农业综合开发有限公司 A kind of mango preservative and preparation method thereof

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Application publication date: 20171226