CN107241981A - A kind of screening technique of the resistance to cadmium of capsicum or low cadmium quality germplasm - Google Patents
A kind of screening technique of the resistance to cadmium of capsicum or low cadmium quality germplasm Download PDFInfo
- Publication number
- CN107241981A CN107241981A CN201710362772.3A CN201710362772A CN107241981A CN 107241981 A CN107241981 A CN 107241981A CN 201710362772 A CN201710362772 A CN 201710362772A CN 107241981 A CN107241981 A CN 107241981A
- Authority
- CN
- China
- Prior art keywords
- cadmium
- capsicum
- resistance
- low
- screening technique
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Soil Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of resistance to cadmium of capsicum or the screening technique of low cadmium quality germplasm, including carrying out germination culture in germinating bed with cadmium solution Stress treatment pepper seed, after culture, by calculating germination percentage, germinating energy and carrying out clustering, germination percentage, germinating energy being reached to, a class capsicum variety of setting data is used as resistance to cadmium germ plasm resource.The present invention screens not only resistance to cadmium but also the capsicum excellent resources of low accumulation cadmium in same batch of hot pepper germ plasm resource, not only ensure capsicum normal growth, in turn ensure that the capsicum of production is poisoned risk after edible without cadmium pollution, and the capsicum variety for further cultivation safety, high yield, high-quality provides excellent germ plasm resource.
Description
Technical field
Field is utilized the present invention relates to capsicum quality germplasm, more particularly to a kind of resistance to cadmium of capsicum or the excellent kind of low cadmium
The screening technique of matter resource.
Background technology
In recent years, how more preferably to ensure that food security remains much-talked-about topic.During arable land cadmium pollution is agroecological environment
The most extensive concern the problem of, it constitutes serious threat to agricultural product quality.Cadmium enters human body by food chain, and damage kidney is small
Pipe, makes skeletal metabolism be obstructed, and causes osteoporosis, shrinkage deformation, seriously endangers health.
Hot pepper germ plasm resource is premise and the basis for carrying out pepper breeding, to accomplish reasonable, the correct profit to source material
With must just the resource collected be identified and evaluated.In order to further utilize, develop these germ plasm resources, improve peppery
Green pepper is kept the safety in production, and reduces the potential heavy metal cadmium risk of human consumption capsicum, thus needs deeply to excavate the capsicum of resistance to cadmium/low cadmium
Quality germplasm.
The content of the invention
It is an object of the invention to provide a kind of resistance to cadmium of capsicum or the screening technique of low cadmium quality germplasm, to solve background
The problem of prior art pointed out in technology is present.
What the present invention was realized in：
The present invention is the screening technique of a kind of resistance to cadmium of capsicum or low cadmium quality germplasm, including uses cadmium solution Stress treatment
Pepper seed carries out germination culture in germinating bed, after culture, by calculating germination percentage, germinating energy and carrying out clustering, will
Germination percentage, germinating energy reach a class capsicum variety of setting data as resistance to cadmium germ plasm resource.
Further the resistance to cadmium germ plasm resource is planted in Cd contaminated soils, it is after harvesting capsicum after maturation, cadmium content is low
Limited the quantity in GB2762-2012 state foods safety standard and provide that 0.05mg/kg, concentration coefficient and transhipment coefficient are peppery less than 1.0
Green pepper kind is used as resistance to cadmium and the quality germplasm of low accumulation cadmium.
Wherein, pepper seed includes line green pepper type or capsicum annum fasciculatum type hot pepper germ plasm resource.Germinating bed is by two qualitative filter papers
It is layered in culture dish and is made.Qualitative filter paper is circular filter paper, a diameter of 9mm.Cd contaminated soils refer to that agricultural land soil Cd contains
Amount exceedes the limitation regulation 0.3mg/kg of national soil quality safety standard, or it is artificial by different content cadmium added to uncontaminated
Formed after soil.Concentration coefficient refers to after capsicum is ripe, the ratio of capsicum cauline leaf and fruit cadmium content and soil cadmium content；Turn
Transport coefficient to refer to after capsicum is ripe, the ratio of capsicum cauline leaf and fruit cadmium content and root cadmium content.
Advantages of the present invention：Not only resistance to cadmium is screened in same batch of hot pepper germ plasm resource by the present invention but also the capsicum of low accumulation cadmium is excellent
Different resource, not only ensures capsicum normal growth, in turn ensures that the capsicum of production is poisoned risk after edible without cadmium pollution, and be
Further cultivate safety, high yield, the capsicum variety of high-quality and excellent germ plasm resource is provided.
Brief description of the drawings
Fig. 1 is Cd stress on different capsicum material germination percentages, the dendrogram of germinating energy influence.
Embodiment
The present invention is described in further detail with reference to the accompanying drawings and examples.
The capsicum quality germplasm of the resistance to cadmium of embodiment 1.：
The present embodiment comprises the following steps：
1. 34 capsicum varieties selected by the present invention are provided by Guizhou Pepper Research Institute, and numbering is P01 respectively,
P0, P02, P03, P04, P05, P06, P07, P08, P09, P010, P011, P012, P013, P014, P015, P016, P017,
P018, P019, P020, P021, P022, P023, P024, P025, P026, P027, P028, P029, P030, P031, P032,
P033, P034.
2. the cadmium solution of 0mg/L, 0.5mg/L, 4.0mg/L, 8.0mg/L concentration is prepared.
3. by step, 2. a series of cadmium concentration solution are added separately to be covered with the qualitative filters of 2 layers of 9mm diameters according to 8mL volumes
In the culture dish of paper, equipped with 1. numbering pepper seed in culture dish.
4. by step constant incubator culture 14 days under the conditions of 3. middle culture dish is put into 28 DEG C ± 1 DEG C, seed is investigated daily
Germinative number (is used as rudiment mark) using radicle up to seed length 1/2, supplement equivalent distilled water and Cd2+The aqueous solution.Every kind of concentration
Cadmium solution processing sets 3 repetitions respectively.
5. 4. calculation procedure calculated germination percentage, germinating energy respectively with the 7th day and the 14th day germinative number.Calculation formula is as follows：
Germination percentage=(Cadmium treated chitting piece number/confession examination chitting piece number) × 100%；Germinating energy=(chitting piece sum/confession in 7d
Plant experimentally sub- sum) × 100%.
6. by step, 5. result of calculation carries out clustering, by pepper seed germination rate, germinating energy all higher (﹥ 50%)
One veriety screens the resistance to cadmium capsicum material number of acquisition for P05, P07, P08, P15, P16 as the hot pepper germ plasm resource of resistance to cadmium,
P34, such as Fig. 1.
The quality germplasm of resistance to/low accumulation cadmium of the capsicum of embodiment 2.：
The present embodiment is on the basis of the hot pepper germ plasm resource of resistance to cadmium, to be sieved after artificial addition different content cadmium to uncontaminated soil
Choosing obtains low cadmium-accumulation capsicum quality germplasm.Comprise the following steps：
1. the yellow earth soil of top layer (0~20cm) without cadmium pollution, and impurities removing are gathered.
2. by various concentrations top pure grade CdCl2·2.5H2O added to step 1. middle soil, simulation soil by 0mg/kg,
0.5mg/kg, 4.0mg/kg, 8.0mg/kg cadmium pollution, and these soil are loaded in basin alms bowl.
3. the hot pepper germ plasm resource of resistance to cadmium screened is planted in step 2. middle soil.
4. after the capsicum of step 3. middle plantation is ripe, harvest and determine pepper root, cadmium contains in cauline leaf, fruit and soil
Amount.
5. according to step, 4. result calculates the concentration coefficient of cadmium and transports coefficient.
6. according to step, 4. 5. result filters out the capsicum quality germplasm of resistance to cadmium/low cadmium with step.
2 kinds of not only resistance to cadmium but also low capsicum materials for accumulating cadmium are filtered out in the present embodiment, numbering is P05, P16, and wherein P05 exists
Cadmium content is respectively in fruit, cauline leaf, root and soil when concentration is 0.5mg/kg：0.048mg/kg, 0.53mg/kg, 0.85mg/
Kg, 0.36mg/kg, concentration coefficient are 0.47, and transhipment coefficient is；Cadmium content is respectively in P16 fruit, cauline leaf, root and soil：
0.036mg/kg, 0.65mg/kg, 0.91mg/kg, 0.54mg/kg.
When concentration is more than more than 0.5mg/kg, pepper fruit content is nuisanceless beyond national agricultural product security quality
Content highest limit value (0.05mg/kg) as defined in vegetable safety requirement, embodiment result shows, the present invention by germination test and
Pot experiment screening obtains not only resistance to cadmium but also the capsicum material of low accumulation cadmium, prevents cadmium from effectively entering pepper fruit, so as to ensure
Vegetable food safety, the material is mainly suitable for planting in low stain soil.
Certainly, it is the concrete application example of the present invention above, the present invention also has other embodiments, all using equivalent
Replacement or the technical scheme of equivalent transformation formation, all fall within protection domain of the presently claimed invention.
Claims (7)
1. the screening technique of a kind of resistance to cadmium of capsicum or low cadmium quality germplasm, it is characterised in that：Including with cadmium solution stress
Reason pepper seed carries out germination culture in germinating bed, after culture, by calculating germination percentage, germinating energy and carrying out clustering,
Germination percentage, germinating energy being reached to, a class capsicum variety of setting data is used as resistance to cadmium germ plasm resource.
2. the screening technique of the resistance to cadmium of capsicum according to claim 1 or low cadmium quality germplasm, it is characterised in that：By institute
State resistance to cadmium germ plasm resource to plant in Cd contaminated soils, after harvesting capsicum after maturation, cadmium content is eaten less than GB2762-2012 countries
The capsicum variety that product safety standard limitation regulation 0.05mg/kg, concentration coefficient and transhipment coefficient are less than 1.0 is used as resistance to cadmium and low product
The quality germplasm of tired cadmium.
3. the screening technique of the resistance to cadmium of capsicum according to claim 2 or low cadmium quality germplasm, it is characterised in that：It is described
Pepper seed includes line green pepper type or capsicum annum fasciculatum type hot pepper germ plasm resource.
4. the screening technique of the resistance to cadmium of capsicum according to claim 2 or low cadmium quality germplasm, it is characterised in that：It is described
Germinating bed is that two qualitative filter papers are layered in culture dish to be made.
5. the screening technique of the resistance to cadmium of capsicum according to claim 4 or low cadmium quality germplasm, it is characterised in that：It is described
Qualitative filter paper is circular filter paper, a diameter of 9mm.
6. the screening technique of the resistance to cadmium of capsicum according to claim 2 or low cadmium quality germplasm, it is characterised in that：It is described
Cd contaminated soils refer to that agricultural land soil Cd contents exceed limitation the regulation 0.3mg/kg, Huo Zheren of national soil quality safety standard
Formed for different content cadmium is added to after uncontaminated soil.
7. the screening technique of the resistance to cadmium of capsicum according to claim 2 or low cadmium quality germplasm, it is characterised in that：It is described
Concentration coefficient refers to after capsicum is ripe, the ratio of capsicum cauline leaf and fruit cadmium content and soil cadmium content；The transhipment coefficient
Refer to after capsicum is ripe, the ratio of capsicum cauline leaf and fruit cadmium content and root cadmium content.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710362772.3A CN107241981B (en) | 2017-05-19 | 2017-05-19 | Method for screening cadmium-resistant or low-cadmium excellent germplasm resources of capsicum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710362772.3A CN107241981B (en) | 2017-05-19 | 2017-05-19 | Method for screening cadmium-resistant or low-cadmium excellent germplasm resources of capsicum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107241981A true CN107241981A (en) | 2017-10-13 |
| CN107241981B CN107241981B (en) | 2021-04-13 |
Family
ID=60017387
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710362772.3A Active CN107241981B (en) | 2017-05-19 | 2017-05-19 | Method for screening cadmium-resistant or low-cadmium excellent germplasm resources of capsicum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107241981B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112680540A (en) * | 2021-01-19 | 2021-04-20 | 贵州省蚕业研究所(贵州省辣椒研究所) | Application of bacterium OM27_ clade in preparation of tool for classifying heavy metal-resistant pepper varieties |
| CN112852982A (en) * | 2021-01-19 | 2021-05-28 | 贵州省蚕业研究所(贵州省辣椒研究所) | Product for classifying cadmium-resistant vegetable varieties |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001089288A1 (en) * | 2000-05-25 | 2001-11-29 | Lestander Torbjoern | Single seed sortation |
| CN101743905A (en) * | 2008-12-05 | 2010-06-23 | 中国科学院沈阳应用生态研究所 | Method for selecting crop varieties with low heavy metal accumulation |
| CN101750472A (en) * | 2008-12-05 | 2010-06-23 | 中国科学院沈阳应用生态研究所 | Method for judging and screening low-accumulation plants |
| CN104472161A (en) * | 2014-11-21 | 2015-04-01 | 沈阳大学 | Method for restoring slight and medium cadmium polluted soil by maize-capsicum intercropping |
| CN105750212A (en) * | 2016-04-20 | 2016-07-13 | 江西农业大学 | Method for screening varieties of heavy metal hyperaccumulation plants |
| CN106069739A (en) * | 2016-06-14 | 2016-11-09 | 中国农业科学院油料作物研究所 | The method of simple and quick high flux screening low heavy metal cadmium accumulation brassica plant |
-
2017
- 2017-05-19 CN CN201710362772.3A patent/CN107241981B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001089288A1 (en) * | 2000-05-25 | 2001-11-29 | Lestander Torbjoern | Single seed sortation |
| CN101743905A (en) * | 2008-12-05 | 2010-06-23 | 中国科学院沈阳应用生态研究所 | Method for selecting crop varieties with low heavy metal accumulation |
| CN101750472A (en) * | 2008-12-05 | 2010-06-23 | 中国科学院沈阳应用生态研究所 | Method for judging and screening low-accumulation plants |
| CN104472161A (en) * | 2014-11-21 | 2015-04-01 | 沈阳大学 | Method for restoring slight and medium cadmium polluted soil by maize-capsicum intercropping |
| CN105750212A (en) * | 2016-04-20 | 2016-07-13 | 江西农业大学 | Method for screening varieties of heavy metal hyperaccumulation plants |
| CN106069739A (en) * | 2016-06-14 | 2016-11-09 | 中国农业科学院油料作物研究所 | The method of simple and quick high flux screening low heavy metal cadmium accumulation brassica plant |
Non-Patent Citations (6)
| Title |
|---|
| 余有见: "不同基因型水稻耐汞性及汞积累差异比较", 《安徽农业科学》 * |
| 廖芳芳等: "镉胁迫对不同品种辣椒种子萌发的影响", 《辣椒杂志》 * |
| 张锡洲等: "水稻镉耐性差异及镉低积累种质资源的筛选", 《中国生态农业学报》 * |
| 沈阳农学院: "《蔬菜育种学》", 31 October 1980 * |
| 邢丹等: "贵州典型土壤－ 辣椒系统中镉的迁移富集特征", 《西南农业学报》 * |
| 龙小林等: "镉胁迫下籼稻和粳稻对镉的吸收、转移和分配研究", 《中国水稻科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112680540A (en) * | 2021-01-19 | 2021-04-20 | 贵州省蚕业研究所(贵州省辣椒研究所) | Application of bacterium OM27_ clade in preparation of tool for classifying heavy metal-resistant pepper varieties |
| CN112852982A (en) * | 2021-01-19 | 2021-05-28 | 贵州省蚕业研究所(贵州省辣椒研究所) | Product for classifying cadmium-resistant vegetable varieties |
| CN112852982B (en) * | 2021-01-19 | 2021-11-30 | 贵州省蚕业研究所(贵州省辣椒研究所) | Product for classifying cadmium-resistant vegetable varieties |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107241981B (en) | 2021-04-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102613056B (en) | Original ecological culture method of dendrobium candidum | |
| CN103947491B (en) | A kind of organic red rice implantation methods | |
| CN101884286B (en) | Method for planting on improved orchard chicken-raising grassland | |
| CN104396535A (en) | Container seedling-raising method for masson pine | |
| Hare et al. | Evaluation of new hybrid brachiaria lines in Thailand. 1. Forage production and quality | |
| Mathias et al. | Potential to increase cassava yields through cattle manure and fertilizer application: Results from Bunda College, Central Malawi | |
| CN107750854A (en) | A kind of cultural method of capsicum | |
| CN106853458A (en) | A kind of method of the low accumulation restoring cadmium polluted vegetable field soil of plantation supporting with enriching plant high | |
| CN103875493B (en) | A kind of organic black rice implantation methods | |
| CN102523863A (en) | Method for cultivating Panax notoginseng by arbuscular mycorrhizal fungi technique | |
| CN104813883B (en) | Method for identifying stability of cadmium accumulation environment of grains of rice varieties | |
| CN103416288B (en) | Soilless culture medium for orchids | |
| CN112514738A (en) | Production method of high-quality phellinus igniarius strain | |
| CN107241981A (en) | A kind of screening technique of the resistance to cadmium of capsicum or low cadmium quality germplasm | |
| CN105646063A (en) | Honey fungus mother strain culture medium, culture method of honey fungus strain, method using cotton straws to produce culture medium, and method using culture medium to cultivate rhizoma gastrodiae | |
| CN106386130A (en) | Method for growing seedlings of baical skullcap root by cutting | |
| CN109748735A (en) | A kind of method of the quick decomposition of green plant stalk and prevention and control flue-cured tobacco root knot nematode disease | |
| CN111374010A (en) | Standardized and efficient glehnia root planting technology | |
| CN109362518A (en) | A kind of method for culturing seedlings of Rhizoma Gastrodiae | |
| CN114946600A (en) | Vegetable seedling raising substrate and preparation method thereof | |
| CN107821132A (en) | A kind of optimal water salt threshold value breeding method of Reed rhizome budding | |
| CN107232019A (en) | Grape stable yields is realized using miscegenation and significantly reduces the implantation methods of its cadmium content | |
| CN114258816A (en) | Planting method of Xinhui oranges | |
| CN106862265B (en) | Method for reducing cadmium absorption and accumulation of rape by using thick-leaf ophiopogon japonicus | |
| CN105016795A (en) | Organic fertilizer prepared from solidago Canadensis and cinnamomum camphora leaf |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |