CN106069739A - The method of simple and quick high flux screening low heavy metal cadmium accumulation brassica plant - Google Patents
The method of simple and quick high flux screening low heavy metal cadmium accumulation brassica plant Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses the method for the low heavy metal cadmium accumulation brassica plant of a kind of simple and quick high flux screening, relate to brassica plant breeding and Screening of Germplasm technical field.This method comprises the following steps: 1. seed disinfection；2. the preparation of germination bed；3. seed is put；4. vernalization process；5. sprout growth；6. radicle length measurement；7. the evaluation of cotyledon period radicle length inhibitory action；8. the brassica plant of low heavy metal cadmium accumulation in group of participating in the experiment is filtered out.The present invention has only to process, with certain density cadmium solution, the brassica plant seed participated in the experiment on germination bed, can filter out the brassica plant of low heavy metal cadmium accumulation, has simple and labour-saving advantage；Screening only needs the time of one week, greatly shortens the screening cycle, accelerates the process of screening；The brassica plant of low heavy metal cadmium accumulation in big colony can be filtered out with high throughput；Do not rely on expensive determining instrument, low cost, it is easy to popularization and application on a large scale.
Description
Technical field
The present invention relates to brassica plant breeding and Screening of Germplasm technical field, particularly relate to a kind of simple and quick height
The method of flux screening low heavy metal cadmium accumulation brassica plant, for screening the excellent brassica plant of low heavy metal cadmium accumulation
Germplasm materials.
Background technology
Cadmium (Cadmium, Cd) is positioned at periodic table of elements period 5 Group IIB, and its molecular weight is 112.41, is that silvery white has
The metal of gloss, is widely used in chemical industry.Heavy metal cadmium is the nonessential metallic element of organism, is to people, animal and to plant
One of heavy metal element of all toxic effect of thing；The heavy metal cadmium of high concentration can suppress growing of plant, causes farming
The yield of thing reduces；In addition, the heavy metal cadmium being accumulated in crops can be enriched in human body by food chain, the body to the mankind
Body health causes serious threat.
With the development of industrialization and urbanization, many countries in the world all suffer from serious cadmium pollution problem.20
50 to the seventies of century, the magic river basin in Toyama County, Japan is due to serious cadmium pollution, and this area there occurs a kind of public hazards
Sick " Itai-itai diseases "；Hereafter, the problem of heavy metal cadmium just causes global extensive concern.In China, 2010 2
Month national environmental protection portion, statistics bureau and the Ministry of Agriculture combine issue " whole nation Pollutant source investigation publication for the first time ", wherein data
Display whole nation heavy metal (cadmium, chromium, arsenic, mercury, lead) gross contamination emission is up to 0.09 ten thousand tons.That issues in April, 2014 is " complete
State's Soil Pollution Investigation publication " display, soil total some position exceeding standard rate in the whole nation is 16.1%, heavy metal cadmium point position exceeding standard rate
Reach 7.0%, occupy first of pollutant；In addition, the soil point position exceeding standard rate ploughed is the highest, reach 19.4%, it is seen then that heavy metal cadmium is
Agricultural production through serious threat to China's most area.
Cabbage type rape in brassica plant is the main oil crops of China, Chinese cabbage in brassica plant, little
Chinese cabbage, wild cabbage, broccoli etc. are main current consumption vegetables, it has been reported that, the main producing region such as Hunan, Jiangxi heavy metal cadmium
Seriously, the safety in production of brassica plant has greatly been threatened.Research shows, heavy metal cadmium can suppress brassica plant
G and D, causes the symptom such as development delay, undergrowth, final reduction brassica plant yield.Research proves, Brassica genus
Heavy metal cadmium in plant easily Enriching soil, cadmium migrates to the quality that edible organs will seriously reduce brassica plant, raw
The agricultural product of output also fail safety standards, and eat by mistake and also can cause injury greatly to human body.
The enrichment of plant heavy metal cadmium does not only exist interspecific difference, there is also significant difference in planting.Low long-pending
The crop germplasm resource of tired heavy metal cadmium screens the research related with the seed selection of new varieties and has become as research outside Present Domestic
One big focus；Previous karyotype studies shows that the accumulation of the crop heavy metal cadmium such as paddy rice, wheat, barley exists in bigger kind
Variation.Screening and the brassica plant material cultivating low heavy metal cadmium accumulation, ensure this important economic work of brassica plant
The safety in production of objects system, will be a vital task of brassica plant Screening of Germplasm and breeding work.Therefore, it is badly in need of
Explore effective approach, in brassica plant germ plasm resource, screen and excavate the excellent resources of low heavy metal cadmium accumulation, energetically
Carry out the cultivation of the brassica plant new varieties of low heavy metal cadmium accumulation.
In tradition screening brassica plant the method for heavy metal cadmium accumulation amount mainly use graphite furnace atomic absorption spectrometry,
The instrument such as flame atomic absorption spectrometry and inductively coupled plasma atomic emission carries out the mensuration of cadmium content, and these methods have
The high advantage of precision；But, its testing cost is very high, instrumentation, sample preparation also extremely complex, be difficult to breeding population
Carry out Large-scale Screening with Germplasms.To this end, set up the low heavy metal cadmium accumulation rue of a kind of simple and quick high flux screening
The method of a kind of sedge platymiscium is possible not only to accelerate screening and the excavation work of low accumulation brassica plant quality germplasm, it is also possible to
Improve the early screening efficiency of low accumulation brassica plant in breeding population, for the Brassica genus promoting heavy metal cadmium area
Plant safety in production plays vital effect.
Content of the invention
The purpose of the present invention is that the shortcoming and defect overcoming prior art to exist, and provides a kind of simple and quick high flux
The method screening low heavy metal cadmium accumulation brassica plant.
The method of the low heavy metal cadmium accumulation brassica plant of screening that the present invention provides comprises the following steps:
1. seed disinfection
Choose full, without disease pest and the some groups of participating in the experiment of uniform brassica plant seed, often organize 300, disappear with 75% alcohol
Poison 5min, and clean with distilled water flushing, and after sterilization terminates, the moisture blotting the surface of the seed with filter paper is standby；
2. the preparation of germination bed
Germination bed is divided into process group germination bed and control group germination bed:
At the culture dish square plastic ware of 12cm (length of side be) 4 layers of qualitative filter paper of middle berth, add again the Cd of 1-50mg/L concentration2+Molten
Liquid 15mL, i.e. described process group germination bed；
At the culture dish square plastic ware of 12cm (length of side be) 4 layers of qualitative filter paper of middle berth, add again equivalent distilled water, i.e. described
Control group germination bed；
Each process group and control group arrange 3 repetitions respectively；
3. seed is put
The seed of step 1. middle sterilization is uniformly placed in step 2. in germinating bed on, bud density be every ware place 50
Seed, and seal culture dish with sealed membrane；
4. vernalization process
It is placed in the culture dish of step 3. middle sealing in growth cabinet, 22 DEG C of dark culturing 2 days, carry out vernalization process；
5. sprout growth
After vernalization terminates, being placed in 22 DEG C of growth cabinets, the photoperiod is to cultivate 5 days under conditions of 16h illumination, 8 h dark；
6. radicle length measurement
Every ware is chosen consistent seedling 10 strain of growth and is measured its radicle length；
7. the evaluation of cotyledon period radicle length inhibitory action
Control group according to step 6. middle measurement and the root length of process group, evaluate cotyledon period radicle under each group Cd stress of participating in the experiment long
The inhibitory action of degree；
8. the brassica plant of low heavy metal cadmium accumulation in group of participating in the experiment is filtered out
The brassica plant that under Cd stress, cotyledon period radicle length inhibitory action is little in group that selects to participate in the experiment is low accumulation heavy metal
The brassica plant of cadmium.
Described step 6. in radicle length refer to that brassica plant cotyledon period seedling is connected with plumular axis from the tip of a root to radicle
Whole length of point, unit is millimeter.
Described step 7. in Cd stress under its evaluation index of inhibitory action of cotyledon period radicle length include cadmium evil coefficient
(CTI, Cadmium Toxicity Index) and relative radicle length (RRL, Relative Radicle Length)；
Wherein: cadmium evil coefficient CTI=(RL_ck-RL_Cd)/RL_ck × 100；
Relative radicle length RRL=RL_Cd/RL_ck；
RL is radicle length,
RL_ck is control group radicle length,
RL_Cd is Cadmium treated group radicle length.
The foundation that 8. described step filters out the brassica plant of low heavy metal cadmium accumulation in group of participating in the experiment is: brassica plant
The accumulation of seed material heavy metal cadmium that cotyledon period radicle length inhibitory action is less under Cd stress is fewer, and radicle length
The accumulation of the bigger material heavy metal cadmium of inhibitory action is more.
The present invention has following advantages and a good effect:
1. have only to process, with certain density cadmium solution, the brassica plant seed participated in the experiment on germination bed, can screen
Go out the brassica plant of low heavy metal cadmium accumulation, relative to the methods such as traditional water planting, earth culture and field production have simple and
Labour-saving advantage；
2. screen the time only needing one week, greatly shorten the screening cycle, accelerate the process of screening；
3. the brassica plant of low heavy metal cadmium accumulation in big colony can be filtered out with high throughput；
4. expensive determining instrument, low cost, it is easy to popularization and application on a large scale are not relied on.
Brief description
Fig. 1 is root measurement method schematic diagram；
Wherein A: radicle and the linking point of plumular axis；B: the tip of a root；C: root length.
Fig. 2 is the schematic diagram of different cabbage type rape variety cadmium content in cotyledon after cadmium solution process.
The abscissa of this figure is cabbage type rape variety, and ordinate is cadmium content (mg/Kg) in cabbage type rape cotyledon；
A1-A4 is 4 cabbage type rape varieties by cadmium inhibitory action little (low accumulation cadmium) for the cotyledon period radicle length；
B1-B4 is 4 cabbage type rape varieties by cadmium inhibitory action big (high accumulation cadmium) for the cotyledon period radicle length.
Fig. 3 is the schematic diagram of different breeds of Chinese cabbage cadmium content in cotyledon after cadmium solution process.
The abscissa of this figure is breeds of Chinese cabbage, and ordinate is cadmium content (mg/Kg) in Chinese cabbage cotyledon；
A1-A4 is 4 breeds of Chinese cabbage by cadmium inhibitory action little (low accumulation cadmium) for the cotyledon period radicle length；
B1-B4 is 4 breeds of Chinese cabbage by cadmium inhibitory action big (high accumulation cadmium) for the cotyledon period radicle length.
Detailed description of the invention:
Describe in detail with embodiment below in conjunction with the accompanying drawings:
Embodiment 1:
With 8 cabbage type rape varieties of measurement at the Cd compareing (distilled water process) and 50mg/L2+Cotyledon period after solution process
Radicle length, coerces down the inhibitory action of cabbage type rape cotyledon period radicle length of participating in the experiment with cadmium evil coefficient for index to cadmium solution
Evaluate, and inhibitory action be classified, filter out the kind of low heavy metal cadmium accumulation in cabbage type rape variety of participating in the experiment,
The detailed description of the invention of the present invention is illustrated；Then Cd accumulation in cotyledon is measured after tried cabbage type rape seed Cd stress
Amount, the accumulation law of heavy metal cadmium in the cabbage type rape cotyledon of the different classification of statistical analysis, verify the screening knot of the present invention
Really.
1st, the specific embodiments screening low accumulation cadmium cabbage type rape variety is as follows:
1) seed disinfection
Choose cabbage type rape variety to be screened full, without each 300 of disease pest, uniform seed, use 75% alcohol disinfecting
5min, and clean with distilled water flushing；After sterilization terminates, the moisture blotting the surface of the seed with filter paper is standby；
2) preparation of germination bed
At the culture dish square plastic ware of 12 cm (length of side be) 4 layers of qualitative filter paper of middle berth, process group adds the Cd of 50 mg/L2+Molten
Liquid 15 mL, control group adds the distilled water of equivalent, and each process arranges 3 repetitions；
3) seed is put
Will in step 1) sterilize after seed be uniformly placed in step 2) in germinating bed on, bud density be every ware place 50
Grain seed, and seal culture dish with sealed membrane；
4) vernalization process
The culture dish sealing in step 3) is placed in growth cabinet 22 DEG C of dark culturing 2 days, carries out vernalization process；
5) sprout growth
After vernalization terminates, being placed in 22 DEG C of growth cabinets, the photoperiod is to cultivate 5 days under conditions of 16 h illumination, 8h dark；
6) cotyledon period radicle length measurement
Every ware is chosen consistent seedling 10 strain of growth and is measured its radicle length；
7) evaluation of cotyledon period radicle length inhibitory action
With Excel 2003 calculate each kind cadmium evil coefficient [CTI=(RL_ck-RL_Cd)/RL_ck × 100, wherein RL is embryo
Root length degree, RL_ck is control group radicle length, and RL_Cd is Cadmium treated group radicle length) and and according to the size pair of cadmium evil coefficient
The repressed degree of radicle length is classified, wherein cadmium evil coefficient less than 50 for A level numbered A1-A4, corresponding cadmium
Evil coefficient more than 50 for B level numbered B1-B4.
Note: this only 8 kinds of test are participated in the experiment, therefore only divide two-stage, if material to be tested colony is very big, can take the circumstances into consideration to carry out
Multiple classifications.
8) material of low heavy metal cadmium accumulation in cabbage type rape of participating in the experiment is filtered out
In the present embodiment, the radicle inhibitory action of this four strains of A1-A4 is less, sweet for the low heavy metal cadmium accumulation that filters out
Blue type rape variety.
2nd, verify that the specific embodiments of the selection result of the present invention is as follows:
1) sample collection
Cd stress processes the seedlings of Brassica napus L of a week, and with distilled water flushing more than 3 times, cleaning surface may the Cd2+ of residual
Blot the moisture on seedling surface, be individually separated the cotyledon of every ware seedling, and be collected in respectively in kraft paper bag；
2) sample drying process
After collection completes, material is placed in baking oven 15 min that complete under the conditions of 105 DEG C, then 60 DEG C of drying 48 in an oven
H is to constant weight；
3) treatments of the sample
The sample comminution that will dry completely, and weigh 0.3-0.5 g(and be designated as G) in polytetrafluoroethylene (PTFE) digest tube, add 5 mL
Mixed acid (HNO3:HClO4=4:1) overnight (12 h) digests；
4) sample disappears and boils
Postdigestive sample, boils as disappearing on electric hot plate, uses and progressively heats up: 60 DEG C, 30 min, 120 DEG C, 30 min, and 160
DEG C, 1 h, 200 DEG C disappear and boil to the transparent clarification of solution；
5) constant volume is filtered
Disappear and boil the solution obtaining and filter in the volumetric flask of 5 mL and standby with distilled water constant volume through funnel.
6) cadmium content measures
Sample after constant volume uses inductively coupled plasma atomic emission (INSTRUMENT MODEL: thermoelectricity Intrepid XSP plasma
Body Atomic Emission Spectrometer AES) detect cadmium metal content (being designated as Cl) therein；
7) in each tissue sample, cadmium content calculates
Calculating each kind cadmium content C in cotyledon under the Cd2+ solution of 50 mg/L is processed, computing formula is: C=Cl*5/G.A level
In (cadmium evil coefficient is less than 50) and B level (cadmium evil coefficient is more than 50) cabbage type rape cotyledon, the average of cadmium content is respectively
163.08、404.52 mg/Kg；
8) data statistic analysis
Cadmium content (see figure 2) rule in statistical analysis A level and B level cabbage type rape cotyledon, according to statistic analysis result, can draw
Conclusion: the cadmium amount that the cabbage type rape variety (A1-A4) of cadmium evil coefficient little (A level, radicle length inhibitory action is little) accumulates is notable
Cabbage type rape variety (B1-B4) less than cadmium evil coefficient big (B level, radicle length inhibitory action is big).
Therefore the screening technique of the present invention is feasible, can filter out cabbage type rape of participating in the experiment simply, quickly and accurately
In the material of low heavy metal cadmium accumulation.
Embodiment 2:
With 8 breeds of Chinese cabbage of measurement at the Cd compareing (distilled water process) and 30mg/L2+Cotyledon period radicle after solution is processed is long
Degree, evaluates with the inhibitory action that cadmium solution is coerced down, for index, Chinese cabbage cotyledon period radicle length of participating in the experiment by cadmium evil coefficient, and
Inhibitory action is classified, filters out the kind of low heavy metal cadmium accumulation in breeds of Chinese cabbage of participating in the experiment, the concrete reality to the present invention
The mode of executing illustrates；Then measuring after try Chinese cabbage seed Cd stress Cd accumulation amount in cotyledon, statistical analysis difference is classified
The accumulation law of heavy metal cadmium in Chinese cabbage cotyledon, verifies the selection result of the present invention.
1st, the specific embodiments screening low accumulation cadmium breeds of Chinese cabbage is as follows:
1) seed disinfection
Choose breeds of Chinese cabbage to be screened full, without each 300 of disease pest, uniform seed, with 75% alcohol disinfecting 5min,
And it is clean with distilled water flushing；After sterilization terminates, the moisture blotting the surface of the seed with filter paper is standby；
2) preparation of germination bed
At the culture dish square plastic ware of 12 cm (length of side be) 4 layers of qualitative filter paper of middle berth, process group adds the Cd of 50 mg/L2+Molten
Liquid 15 mL, control group adds the distilled water of equivalent, and each process arranges 3 repetitions；
3) seed is put
Will in step 1) sterilize after seed be uniformly placed in step 2) in germinating bed on, bud density be every ware place 50
Grain seed, and seal culture dish with sealed membrane；
4) vernalization process
The culture dish sealing in step 3) is placed in growth cabinet 22 DEG C of dark culturing 2 days, carries out vernalization process；
5) sprout growth
After vernalization terminates, being placed in 22 DEG C of growth cabinets, the photoperiod is to cultivate 5 days under conditions of 16 h illumination, 8h dark；
6) cotyledon period radicle length measurement
Every ware is chosen consistent seedling 10 strain of growth and is measured its radicle length；
7) evaluation of cotyledon period radicle length inhibitory action
With Excel 2003 calculate each kind cadmium evil coefficient [CTI=(RL_ck-RL_Cd)/RL_ck × 100, wherein RL is embryo
Root length degree, RL_ck is control group radicle length, and RL_Cd is Cadmium treated group radicle length) and and according to the size pair of cadmium evil coefficient
The repressed degree of radicle length is classified, wherein cadmium evil coefficient less than 50 for A level numbered A1-A4, corresponding cadmium
Evil coefficient more than 50 for B level numbered B1-B4.
Note: this only 8 kinds of test are participated in the experiment, therefore only divide two-stage, if material to be tested colony is very big, can take the circumstances into consideration to carry out
Multiple classifications.
8) material of low heavy metal cadmium accumulation in Chinese cabbage of participating in the experiment is filtered out
In the present embodiment, the radicle inhibitory action of this four strains of A1-A4 is less, white for the low heavy metal cadmium accumulation that filters out
Vegetable kind.
2nd, verify that the specific embodiments of the selection result of the present invention is as follows:
1) sample collection
Cd stress processes the Chinese cabbage seedling of a week, with distilled water flushing more than 3 times, cleans surface and the Cd2+ of residual may blot children
The moisture on seedling surface, is individually separated the cotyledon of every ware seedling, and is collected in respectively in kraft paper bag；
2) sample drying process
After collection completes, material is placed in baking oven 15 min that complete under the conditions of 105 DEG C, then 60 DEG C of drying 48 in an oven
H is to constant weight；
3) treatments of the sample
The sample comminution that will dry completely, and weigh 0.3-0.5 g(and be designated as G) in polytetrafluoroethylene (PTFE) digest tube, add 5 mL
Mixed acid (HNO3:HClO4=4:1) overnight (12 h) digests；
4) sample disappears and boils
Postdigestive sample, boils as disappearing on electric hot plate, uses and progressively heats up: 60 DEG C, 30 min, 120 DEG C, 30 min, and 160
DEG C, 1 h, 200 DEG C disappear and boil to the transparent clarification of solution；
5) constant volume is filtered
Disappear and boil the solution obtaining and filter in the volumetric flask of 5 mL and standby with distilled water constant volume through funnel.
6) cadmium content measures
Sample after constant volume uses inductively coupled plasma atomic emission (INSTRUMENT MODEL: thermoelectricity Intrepid XSP plasma
Body Atomic Emission Spectrometer AES) detect cadmium metal content (being designated as Cl) therein；
7) in each tissue sample, cadmium content calculates
Calculating each kind cadmium content C in cotyledon under the Cd2+ solution of 30 mg/L is processed, computing formula is: C=Cl*5/G.A level
In (cadmium evil coefficient is less than 50) and B level (cadmium evil coefficient is more than 50) Chinese cabbage cotyledon, the 315.35th, the average of cadmium content is respectively
505.38 mg/Kg；
8) data statistic analysis
Cadmium content (see figure 2) rule in statistical analysis A level and B level Chinese cabbage cotyledon, according to statistic analysis result, it may be concluded that
The cadmium amount that the breeds of Chinese cabbage (A1-A4) of cadmium evil coefficient little (A level, radicle length inhibitory action is little) accumulates is substantially less than cadmium evil coefficient
The breeds of Chinese cabbage (B1-B4) of (B level, radicle length inhibitory action is big) greatly.
Therefore the screening technique of the present invention is feasible, can filter out in Chinese cabbage of participating in the experiment low long-pending simply, quickly and accurately
The material of tired heavy metal cadmium.
Claims (4)
1. the method for the low heavy metal cadmium accumulation brassica plant of a simple and quick high flux screening, it is characterised in that:
1. seed disinfection
Choose full, without disease pest and the some groups of participating in the experiment of uniform brassica plant seed, often organize 300, disappear with 75% alcohol
Poison 5min, and clean with distilled water flushing, and after sterilization terminates, the moisture blotting the surface of the seed with filter paper is standby；
2. the preparation of germination bed
Germination bed is divided into process group germination bed and control group germination bed:
At square plastic 4 layers of qualitative filter paper of ware middle berth that the culture dish i.e. length of side is 12cm, add again the Cd of 1-50mg/L concentration2+Molten
Liquid 15mL, i.e. described process group germination bed；
At square plastic 4 layers of qualitative filter paper of ware middle berth that the culture dish i.e. length of side is 12cm, add again equivalent distilled water, i.e. described
Control group germination bed；
Each process group and control group arrange 3 repetitions respectively；
3. seed is put
The seed of step 1. middle sterilization is uniformly placed in step 2. in germinating bed on, bud density be every ware place 50
Seed, and seal culture dish with sealed membrane；
4. vernalization process
It is placed in the culture dish of step 3. middle sealing in growth cabinet, 22 DEG C of dark culturing 2 days, carry out vernalization process；
5. sprout growth
After vernalization terminates, being placed in 22 DEG C of growth cabinets, the photoperiod is to cultivate 5 days under conditions of 16h illumination, 8 h dark；
6. radicle length measurement
Every ware is chosen consistent seedling 10 strain of growth and is measured its radicle length；
7. the evaluation of cotyledon period radicle length inhibitory action
Control group according to step 6. middle measurement and the root length of process group, evaluate cotyledon period radicle under each group Cd stress of participating in the experiment long
The inhibitory action of degree；
8. the brassica plant of low heavy metal cadmium accumulation in group of participating in the experiment is filtered out
The brassica plant that under Cd stress, cotyledon period radicle length inhibitory action is little in group that selects to participate in the experiment is low accumulation heavy metal
The brassica plant of cadmium.
2. the method for the low heavy metal cadmium accumulation brassica plant of screening as described in claim 1, it is characterised in that:
Described step 6. in radicle length refer to brassica plant cotyledon period seedling from the tip of a root to radicle with plumular axis tie point
Whole length, unit is millimeter.
3. the method for the low heavy metal cadmium accumulation brassica plant of screening as described in claim 1, it is characterised in that:
Described step 7. in Cd stress under its evaluation index of inhibitory action of cotyledon period radicle length include cadmium evil coefficient CTI and
Relative radicle length RRL；
Wherein: cadmium evil coefficient CTI=(RL_ck-RL_Cd)/RL_ck × 100；
Relative radicle length RRL=RL_Cd/RL_ck；
RL is radicle length,
RL_ck is control group radicle length,
RL_Cd is Cadmium treated group radicle length.
4. the method for the low heavy metal cadmium accumulation brassica plant of screening as described in claim 1, it is characterised in that:
The foundation that 8. described step filters out the brassica plant of low heavy metal cadmium accumulation in group of participating in the experiment is: brassica plant seed
Under Cd stress, the accumulation of the material heavy metal cadmium that cotyledon period radicle length inhibitory action is less is fewer, and radicle length suppression
The accumulation acting on bigger material heavy metal cadmium is more.
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| CN107130036A (en) * | 2017-05-31 | 2017-09-05 | 湖南科技大学 | A kind of gene and method for quickly judging low accumulation cadmium brassica plant |
| CN107241981A (en) * | 2017-05-19 | 2017-10-13 | 贵州省蚕业研究所 | A kind of screening technique of the resistance to cadmium of capsicum or low cadmium quality germplasm |
| CN107262388A (en) * | 2017-08-03 | 2017-10-20 | 河北农业大学 | Method for rapidly screening low-accumulation heavy metal crop varieties and device used by same |
| CN107607700A (en) * | 2017-09-08 | 2018-01-19 | 中国农业科学院棉花研究所 | A kind of authentication method of the resistance to cadmium of cotton |
| CN109618592A (en) * | 2018-12-03 | 2019-04-16 | 南京迪天高新产业技术研究院有限公司 | A method of screening the rice varieties of low accumulation toxic heavy metal |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107241981A (en) * | 2017-05-19 | 2017-10-13 | 贵州省蚕业研究所 | A kind of screening technique of the resistance to cadmium of capsicum or low cadmium quality germplasm |
| CN107241981B (en) * | 2017-05-19 | 2021-04-13 | 贵州省蚕业研究所 | Method for screening cadmium-resistant or low-cadmium excellent germplasm resources of capsicum |
| CN107130036A (en) * | 2017-05-31 | 2017-09-05 | 湖南科技大学 | A kind of gene and method for quickly judging low accumulation cadmium brassica plant |
| CN107262388A (en) * | 2017-08-03 | 2017-10-20 | 河北农业大学 | Method for rapidly screening low-accumulation heavy metal crop varieties and device used by same |
| CN107607700A (en) * | 2017-09-08 | 2018-01-19 | 中国农业科学院棉花研究所 | A kind of authentication method of the resistance to cadmium of cotton |
| CN107607700B (en) * | 2017-09-08 | 2019-06-18 | 中国农业科学院棉花研究所 | A kind of identification method of the resistance to cadmium of cotton |
| CN109618592A (en) * | 2018-12-03 | 2019-04-16 | 南京迪天高新产业技术研究院有限公司 | A method of screening the rice varieties of low accumulation toxic heavy metal |
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