CN109618592A - A method of screening the rice varieties of low accumulation toxic heavy metal - Google Patents
A method of screening the rice varieties of low accumulation toxic heavy metal Download PDFInfo
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- CN109618592A CN109618592A CN201811465244.1A CN201811465244A CN109618592A CN 109618592 A CN109618592 A CN 109618592A CN 201811465244 A CN201811465244 A CN 201811465244A CN 109618592 A CN109618592 A CN 109618592A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of methods of rice varieties for screening low accumulation toxic heavy metal, belong to agricultural technology and ecological technique interleaving techniques field, the screening technique mainly includes the improvement to nutrient solution prescription, and carrying out disinfection to seed is added heavy metal in sterilization, vernalization, the operating method transplanted seedlings and the nutrient solution for testing initial stage and more meets the actual conditions of daily Rice Cropping；Using the accurate rice varieties for filtering out low accumulation toxic heavy metal of shorter time, the present invention is tested using rice seedling, and experimental period is short, and experiment effect is significant；The experimental method that the present invention uses, required experimental site is small, easy to operate, experimental cost is small, data analysis is quick.
Description
Technical field
The present invention relates to agricultural technology and ecological technique interleaving techniques field more particularly to a kind of low accumulation of screening are toxic heavy
The method of the rice varieties of metal.
Background technique
It is regional or repaired in middle low pollution but since various factors is without that can reach " standard of soil environment quality
GB15618-1995 " agricultural land soil on, planting low accumulation rice can make rice meet " national food safety standard-food
Middle pollutant limitation " (GB2762-2012).
The existing method for screening low accumulation heavy metal crop varieties has field trial, greenhouse earth culture and laboratory water planting.
Process required for field trial screens is very long, and external environment is affected；Greenhouse earth culture crop, broadcasts in solid culture medium
It when kind is not easy that every seed is marked or markers work amount is too big, crop seedling root and ground during transplanting
Portion is vulnerable to damage, the more difficult complete root of harvest when plant harvest, and complicated component in soil and nutritive cube is not easy to control,
There is certain limitation for experimental section；Laboratory water planting relative velocity is quicker but answering in view of soil constituent
It is miscellaneous, so adding culture medium only with water planting is the practical growing environment for having no idea to simulate crop completely, equally in plant
Cannot be guaranteed that the root of plant is completely harvested when harvest, late stage of culture is added heavy metal and does not meet practical condition, and
And incubation time is in contrast still longer.
Summary of the invention
For the existing deficiency for quickly screening low accumulation heavy metal crop method, the present invention puts forward a kind of quick, letter
Just, the method for the rice varieties of the low accumulation toxic heavy metal of novel screening.
To achieve the goals above, the technical solution adopted in the present invention is as follows:
A method of screening the rice varieties of low accumulation toxic heavy metal, comprising the following steps:
Step 1: the rice nutrition formula recommended International Rice Research Institute is improved, and addition is higher than 1-3 times of soil background
Concentration toxic heavy metal compound configure culture solution, and adjust pH be 5.5；
Step 2: seed disinfection to be screened is sterilized；
Step 3: seed to be screened being subjected to vernalization 48h, vernalization is using the synchronous germinator of sort-type to rice seed to be screened
Son synchronizes vernalization；
Step 4: selection grows consistent young shoot and transplants seedlings, and transplants seedlings to in porose PVC board, and put it into
It is cultivated in PVC container equipped with nutrient solution, adjusts the condition of illumination box are as follows: day/night duration 14/10h, temperature are
28/20 DEG C, all laboratory samples are transferred in incubator and cultivate by humidity 70%；
Step 5: sampling, first separate the root leaf of rice seedling, then be washed with deionized water, by root after rice seedling culture 48h
System is placed in 20mM disodium ethylene diamine tetraacetate and impregnates 30min, removes the metal ion of adsorption, is then cleaned with ultrapure water,
The moisture of root surface is blotted, the root leaf after cleaning is put into 100 DEG C of water-removings in baking oven, and 70 DEG C drying to constant weight；
Step 6: after sample drying, using nitric acid heating board digestion, with Cr in atomic absorption spectrophotometer measurement digestion solution
Content；
Step 7: cell membrane surface potential is calculated by GCS model and Nernst equation, according to calculate resulting transfer ratio with
And in cell membrane surface potential and solution various zwitterions concentration, the transfer of heavy metal is calculated using SGCD software
The accumulation of amount, each section filters out the low rice varieties for accumulating toxic heavy metal using comparing.
Preferably, the nutrient solution prescription in the step 1 are as follows: 40mg/L NH4NO3、10mg/L NaH2PO4·2H2O、
40mg/L K2SO4、40mg/L CaCl2、40mg/L MgSO4·7H2O、0.5mg/L MnCl2·4H2O、 0.05mg/L
(NH4)6Mo7O24·2H2O、0.2mg/L H3BO3、0.01mg/L ZnSO4·7H2O、0.01mg/L CuSO4·5H2O、2mg/L
FeCl3·6H2O、300mg/L CrCl3。
Preferably, the heavy metal of the addition is one or more of Cd, Hg, As, Pb, Cr, the Cd concentration is
0.3 ~ 0.9mg/L, Hg concentration are 0.3 ~ 0.9mg/L, As concentration is 30 ~ 90mg/L, Pb concentration is 80 ~ 240mg/L, Cr concentration is
150~450mg/L。
Preferably, the concentration toxic heavy metal chemical combination that the concentration of added heavy metal is 3 times higher than soil background
Object configures culture solution.
Preferably, 0.5% sodium hypochlorite of rice paddy seed is impregnated 30min, disinfection and sterilization in the step 2.
Preferably, the synchronous germinator of sort-type in the step 3 includes tray rack and is set in the tray rack
Several tray bodies, the tray rack is rectangular tray rack, and several tray bodies are located in the tray rack side by side up and down,
The different rice varieties of vernalization in every layer of tray body.
Preferably, the height of each tray body in the tray rack can up and down adjustment, each tray body
On also set up multiple ventilation holes, pallet lid is adapted on the tray rack, heating element is set on the pallet lid, is heated
Element is located in tray rack.
Preferably, being machined with lattice blend stop on the top surface of each tray body, and each lattice blend stop two sides
Slot is squeezed integrally formed with arc, is machined on the bottom surface of each tray body inverted U-shaped with lattice blend stop cooperation
Locking teeth.
Preferably, forming germinating cabinet between lattice blend stop described in every two, bottom is laid with 3-4 layers in each germinating cabinet
Filter paper is soaked with water before being put into seed, then the rice paddy seed sterilized is laid on filter paper, then planted by filter paper
Bedding 1-2 layers of pure water wet tissue on son, and a tray body on upper layer is moved down, the inverted U-shaped lock of upper tray body bottom
Tight tooth is clamped on the lattice blend stop of lower tray body top surface, and the arc of dative blend stop two sides squeezes slot and corresponds to clamping, then again
One layer of water is sprinkled in pure water wet tissue.
Preferably, the vernalization parameter in pregermination procedure in tray rack are as follows: urged under 32 DEG C, the dark condition of 80% humidity
Bud 48h, every 4h watering keep rice paddy seed to be in more wet environment, pass through the heating element control point on pallet lid
Temperature in the synchronous germinator of class formula.
The beneficial effects of the present invention are:
(1) present invention to seed carry out disinfection sterilization, vernalization, the operating method transplanted seedlings and test initial stage nutrient solution in be added
Heavy metal more meets the actual conditions of daily Rice Cropping；
(2) present invention is tested using rice seedling, and experimental period is short, and experiment effect is significant；
(3) experimental method that the present invention uses, required experimental site is small, easy to operate, experimental cost is small, data analysis is fast
It is prompt.
The experimental method that the present invention uses, required experimental site is small, easy to operate, experimental cost is small, data analysis
Fast.
(4) it is screened the present invention be directed to rice varieties, therefore present invention employs sort-types to synchronize in the vernalization stage
The synchronous progress vernalization simultaneously of a variety of rice varieties to be screened can be reduced error brought by traditional vernalization mode by germinator, and
And the germination accelerating method can be realized quick pregermination by the way of filter paper combination pure water wet tissue, more traditional germination time shortens
Half.
Detailed description of the invention
Fig. 1 is the cadmium content comparison diagram after four kinds of rice varieties experiments after present invention addition heavy metal cadmium.
Fig. 2 is the schematic front view of the synchronous germinator of sort-type of the present invention.
Fig. 3 is the schematic front view of the synchronous germinator of sort-type of the present invention.
Fig. 4 is the top view of tray rack of the present invention.
Wherein: 1- tray rack, 2- tray body, 21- ventilation hole, 22- locking teeth, 23- lattice blend stop, 24- arc squeeze slot,
25- guide pad, 3- regulating tank, 4- pallet lid, 5- heating element.
Specific embodiment
In order to make those skilled in the art be better understood on technical solution of the present invention, with reference to the accompanying drawing and
Embodiment is further described technical solution of the present invention.
Time-consuming, experiment is complicated, heavy workload for the existing method for screening low accumulation heavy metal rice.It is missed to reduce experiment
Difference shortens experimental period, reduces workload, and the present invention provides a kind of novel side for screening low accumulation heavy metal rice varieties
Method.
A method of screening the rice varieties of low accumulation toxic heavy metal, comprising the following steps:
Step 1: the rice nutrition formula recommended International Rice Research Institute is improved, and addition is higher than 1-3 times of soil background
Concentration toxic heavy metal compound configure culture solution, specifically can be used 3 times concentration toxic heavy metal compound configuration
Culture solution, and adjusting pH is 5.5；
Nutrient solution prescription are as follows: 40mg/L NH4NO3、10mg/L NaH2PO4·2H2O、40mg/L K2SO4、40mg/L CaCl2、
40mg/L MgSO4·7H2O、0.5mg/L MnCl2·4H2O、 0.05mg/L (NH4)6Mo7O24·2H2O、0.2mg/L
H3BO3、0.01mg/L ZnSO4·7H2O、0.01mg/L CuSO4·5H2O、2mg/L FeCl3·6H2O、300mg/L CrCl3；
Some in the rice special nutrient solution that International Rice research institute in the step 1 is recommended are at used in branch and experiment
Heavy metal chemically reacts, and influences the function and effect of zwitterion, changes tool into so needing to be adjusted according to the actual situation
There is the reagent of same effect to replace, added heavy metal concentration concentration according to set by heavy metal element difference in nutrient solution
Also different, set concentration eats rapid test paper soil quality assessment standard " HJ/T332-2006 " with China, in conjunction with water
The pH value of rice planting soil, setting concentration are 1 ~ 3 times of heavy metal-polluted soil bio-available Zn concentration；
The heavy metal of the addition is one or more of Cd, Hg, As, Pb, Cr, and the Cd concentration is 0.3 ~ 0.9mg/L, Hg
Concentration is 0.3 ~ 0.9mg/L, As concentration is 30 ~ 90mg/L, Pb concentration is 80 ~ 240mg/L, Cr concentration is 150 ~ 450mg/L；
Step 2: seed disinfection to be screened being sterilized, 0.5% sodium hypochlorite of rice paddy seed is impregnated into 30min；
Step 3: seed to be screened being subjected to vernalization 48h, vernalization is using the synchronous germinator of sort-type to rice seed to be screened
Son synchronizes vernalization；
Referring to shown in attached drawing 2-3, if the synchronous germinator of sort-type includes tray rack 1 and the dry care in the tray rack 1
Disk body 2, the tray rack 1 are rectangular tray rack 1, and several about 2 tray bodies are located at side by side in the tray rack 1, every
The different rice varieties of vernalization in the layer tray body 2；The height of each tray body 2 can be upper and lower in the tray rack 1
It adjusts, mainly by being integrally formed guide pad 25 on tray body 2, and opens regulating tank 3 on the inner wall of tray rack 1, adjust
The sliding up and down to adjust in regulating tank 3 by guide pad 25 in the process, guide pad 25 is stuck in regulating tank 3, each
Multiple ventilation holes 21 are also set up on the tray body 2, pallet lid 4 are adapted on the tray rack 1, on the pallet lid 4
Heating element 5 is set, heating element 5 is located in tray rack 1, it is machined with lattice blend stop 23 on the top surface of each tray body 2,
And each 23 two sides of lattice blend stop squeeze slot 24 integrally formed with arc, add on the bottom surface of each tray body 2
Work has the inverted U-shaped locking teeth 22 with the lattice blend stop 23 cooperation, forms germinating cabinet between lattice blend stop 23 described in every two, each
Bottom is laid with 3-4 layers of filter paper and soaks filter paper with water, the rice that then will be sterilized before being put into seed in the germinating cabinet
Seed is laid on filter paper, then the bedding 1-2 layers of pure water wet tissue on seed, and a tray body 2 on upper layer is moved down,
The inverted U-shaped locking teeth 22 of 2 bottom of upper tray body is clamped on the lattice blend stop 23 of 2 top surface of lower tray body, and dative blend stop
The arc of 23 two sides squeezes the corresponding clamping of slot 24, then sprinkles one layer of water in pure water wet tissue again；
The purpose of the synchronous germinator setting of sort-type is being capable of the same stepping in same environment by rice paddy seed to be screened simultaneously
Row vernalization would not have vernalization error in such pregermination procedure, because being all derived from identical vernalization environment, and use this hair
The material of the synchronous germinator of bright sort-type moisturizing in use is pure water wet tissue, that is, the wet of any additive is not added
The water performance of towel, wet tissue is good, and moistening effect is stronger, and the factor that Germination effect is influenced in pregermination procedure is exactly moisture,
The present invention uses filter paper in the bottom of tray body 2 to improve the effect of vernalization and shorten the time of vernalization, makes at top
With pure water wet tissue, and cause moisture rapidly losing in order to avoid there are hole in wet tissue two sides after wet tissue is laid on the surface of the seed,
Therefore the present invention devises arc on locking teeth 22 and lattice blend stop 23 and squeezes the structure that slot 24 matches, after wet tissue is laid, under
Upper tray body 2 is moved, the locking teeth 22 of 2 bottom surface of upper tray body is stuck on the lattice blend stop 23 of 2 top surface of lower tray body, is locked
Tooth 22 is inverted U-shaped, and two sides leg bottom surface is also that the arcwall face that slot 24 cooperates is squeezed with arc, and arcwall face and arc squeeze
Slot 24 is corresponding, can be by two quick clampings in side of pure water wet tissue, and there is no biggish gaps on both sides；Secondly, wet tissue also has
There is preferable gas permeability, does not influence the respiration of seed；Again, the germinator of block form up and down of the invention can be placed simultaneously
Multiple layer tray body 2, a kind of rice paddy seed of vernalization on every layer of tray body 2, the rice of the convenient multiple kinds of vernalization simultaneously；
Vernalization parameter in pregermination procedure in tray rack are as follows: vernalization 48h, every 4h are poured under 32 DEG C, the dark condition of 80% humidity
Water keeps rice paddy seed to be in more wet environment, passes through the synchronous vernalization of heating element control tactics formula on pallet lid
Temperature in device；It, can in order to avoid the ingredient of pure water wet tissue causes some influences to the screening of seed when using pure water wet tissue
Pure water wet tissue is being steeped the sterilization that carries out disinfection in 0.5% liquor natrii hypochloritis using preceding, reuse, it is ensured that in use process
The effect of pure water wet tissue is moisturizing and water；
Step 4: selection grows consistent young shoot and transplants seedlings, and transplants seedlings to in porose PVC board, and put it into
It is cultivated in PVC container equipped with nutrient solution, adjusts the condition of illumination box are as follows: day/night duration 14/10h, temperature are
28/20 DEG C, all laboratory samples are transferred in incubator and cultivate by humidity 70%；
Step 5: sampling, first separate the root leaf of rice seedling, then be washed with deionized water, by root after rice seedling culture 48h
System is placed in 20mM disodium ethylene diamine tetraacetate and impregnates 30min, removes the metal ion of adsorption, is then cleaned with ultrapure water,
The moisture of root surface is blotted, the root leaf after cleaning is put into 100 DEG C of water-removings in baking oven, and 70 DEG C drying to constant weight；
Step 6: after sample drying, using nitric acid heating board digestion, with Cr in atomic absorption spectrophotometer measurement digestion solution
Content；
Step 7: cell membrane surface potential is calculated by GCS model and Nernst equation, according to calculate resulting transfer ratio with
And in cell membrane surface potential and solution various zwitterions concentration, the transfer of heavy metal is calculated using SGCD software
The accumulation of amount, each section filters out the low rice varieties for accumulating toxic heavy metal using comparing；
Transfer ratio=aerial part content of beary metal/under ground portion content of beary metal
Nernst equation: Ψ=Ψ(standard)+ (0.0592/n)/lg([oxidized form]/[reduced form])
In formula: n-electron transfer number
[oxidized form]/[reduced form]-indicates to participate in the product and reactant concentration product of electrode reaction all substances concentration
The ratio between.
The method provided by the invention for screening low accumulation toxic heavy metal rice varieties, it is toxic can to screen low accumulation simultaneously
The rice varieties of heavy metal also will appreciate that toxic heavy metal to the toxicity of rice.The culture rice by the way of water planting, Ji Nengwei
Paddy growth provides enough nutrition and moisture, just adds heavy metal at experiment paddy growth initial stage and is more in line with actual conditions,
Soil will not be checked in practical planting process, select the soil breeding or plantation of not heavy metal pollution.
As shown in Figure 1, each rice varieties have three histograms, leftmost histogram is represented in three histograms
It is root cadmium content, what middle column figure represented is cauline leaf cadmium content, and the histogram of rightmost is cadmium content in seed.
Table one, table two, table three, table four are set forth what use screening technique of the present invention screened four heavy metal species
Content of beary metal table in seedling.
Table one
Table two
Table three
Table four
The basic principles, main features and advantages of the present invention have been shown and described above.The technical staff of the industry
It should be appreciated that the present invention is not limited to the above embodiments, the above embodiments and description only describe the present invention
Principle, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these variation and
Improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent
Object defines.
Claims (10)
1. a kind of method for the rice varieties for screening low accumulation toxic heavy metal, which comprises the following steps:
Step 1: the rice nutrition formula recommended International Rice Research Institute is improved, and addition is higher than 1-3 times of soil background
Concentration toxic heavy metal compound configure culture solution, and adjust pH be 5.5；
Step 2: seed disinfection to be screened is sterilized；
Step 3: seed to be screened being subjected to vernalization 48h, vernalization is using the synchronous germinator of sort-type to rice seed to be screened
Son synchronizes vernalization；
Step 4: selection grows consistent young shoot and transplants seedlings, and transplants seedlings to in porose PVC board, and put it into
It is cultivated in PVC container equipped with nutrient solution, adjusts the condition of illumination box are as follows: day/night duration 14/10h, temperature are
28/20 DEG C, all laboratory samples are transferred in incubator and cultivate by humidity 70%；
Step 5: sampling, first separate the root leaf of rice seedling, then be washed with deionized water, by root after rice seedling culture 48h
System is placed in 20mM disodium ethylene diamine tetraacetate and impregnates 30min, removes the metal ion of adsorption, is then cleaned with ultrapure water,
The moisture of root surface is blotted, the root leaf after cleaning is put into 100 DEG C of water-removings in baking oven, and 70 DEG C drying to constant weight；
Step 6: after sample drying, using nitric acid heating board digestion, with Cr in atomic absorption spectrophotometer measurement digestion solution
Content；
Step 7: cell membrane surface potential is calculated by GCS model and Nernst equation, according to calculate resulting transfer ratio with
And in cell membrane surface potential and solution various zwitterions concentration, the transfer of heavy metal is calculated using SGCD software
The accumulation of amount, each section filters out the low rice varieties for accumulating toxic heavy metal using comparing；
Transfer ratio=aerial part content of beary metal/under ground portion content of beary metal
Nernst equation: Ψ=Ψ(standard)+ (0.0592/n)/lg([oxidized form]/[reduced form])
In formula: n-electron transfer number
[oxidized form]/[reduced form]-indicates to participate in the product and reactant concentration product of electrode reaction all substances concentration
The ratio between.
2. a kind of method of rice varieties for screening low accumulation toxic heavy metal according to claim 1, it is characterised in that:
Nutrient solution prescription in the step 1 are as follows: 40mg/L NH4NO3、10mg/L NaH2PO4·2H2O、40mg/L K2SO4、40mg/
L CaCl2、40mg/L MgSO4·7H2O、0.5mg/L MnCl2·4H2O、 0.05mg/L (NH4)6Mo7O24·2H2O、
0.2mg/L H3BO3、0.01mg/L ZnSO4·7H2O、0.01mg/L CuSO4·5H2O、2mg/L FeCl3·6H2O、300mg/
L CrCl3。
3. a kind of method of rice varieties for screening low accumulation toxic heavy metal according to claim 1, it is characterised in that:
The heavy metal of the addition is one or more of Cd, Hg, As, Pb, Cr, and the Cd concentration is 0.3 ~ 0.9mg/L, Hg concentration
For 0.3 ~ 0.9mg/L, As concentration be 30 ~ 90mg/L, Pb concentration is 80 ~ 240mg/L, Cr concentration is 150 ~ 450mg/L.
4. a kind of method of rice varieties for screening low accumulation toxic heavy metal according to claim 3, it is characterised in that:
The concentration toxic heavy metal compound that the concentration of added heavy metal is 3 times higher than soil background configures culture solution.
5. a kind of method of rice varieties for screening low accumulation toxic heavy metal according to claim 1, it is characterised in that:
0.5% sodium hypochlorite of rice paddy seed is impregnated into 30min, disinfection and sterilization in the step 2.
6. a kind of method of rice varieties for screening low accumulation toxic heavy metal according to claim 1, it is characterised in that:
The synchronous germinator of sort-type in the step 3 includes tray rack and several tray bodies in the tray rack, described
Tray rack is rectangular tray rack, and several tray bodies are located in the tray rack side by side up and down, in every layer of tray body
The different rice varieties of vernalization.
7. a kind of method of rice varieties for screening low accumulation toxic heavy metal according to claim 6, it is characterised in that:
The height of each tray body in the tray rack can up and down adjustment, also set up multiple ventilations on each tray body
Hole is adapted to pallet lid on the tray rack, heating element is arranged on the pallet lid, heating element is located at tray rack
It is interior.
8. a kind of method of rice varieties for screening low accumulation toxic heavy metal according to claim 7, it is characterised in that:
Lattice blend stop is machined on the top surface of each tray body, and each lattice blend stop two sides are squeezed integrally formed with arc
Indent is machined with the inverted U-shaped locking teeth with lattice blend stop cooperation on the bottom surface of each tray body.
9. a kind of method of rice varieties for screening low accumulation toxic heavy metal according to claim 8, it is characterised in that:
Germinating cabinet is formed between lattice blend stop described in every two, bottom is laid with 3-4 layers of filter paper in each germinating cabinet, is being put into seed
Before, filter paper is soaked with water, then the rice paddy seed sterilized is laid on filter paper, then on seed bedding 1-2 layers it is pure
Water-wet towel, and a tray body on upper layer is moved down, the inverted U-shaped locking teeth of upper tray body bottom is clamped on lower layer's support
On the lattice blend stop of disk body top surface, and the arc of dative blend stop two sides squeezes slot and corresponds to clamping, then sprinkles in pure water wet tissue again
One layer of water.
10. a kind of method of rice varieties for screening low accumulation toxic heavy metal according to claim 9, feature exist
In vernalization parameter in pregermination procedure in tray rack are as follows: the vernalization 48h under 32 DEG C, the dark condition of 80% humidity, every 4h watering,
Rice paddy seed is kept to be in more wet environment, by the synchronous germinator of heating element control tactics formula on pallet lid
Temperature.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112219703A (en) * | 2020-10-28 | 2021-01-15 | 武汉市秀谷科技有限公司 | Planting and cultivating method aiming at low accumulation of heavy metals in farmland |
| CN115156286A (en) * | 2022-07-01 | 2022-10-11 | 贵州师范大学 | Method for efficiently screening heavy metal low-accumulation ecological restoration plants/crops |
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| CN115156286A (en) * | 2022-07-01 | 2022-10-11 | 贵州师范大学 | Method for efficiently screening heavy metal low-accumulation ecological restoration plants/crops |
| CN115156286B (en) * | 2022-07-01 | 2024-05-10 | 贵州师范大学 | Efficient screening method for heavy metal low-accumulation ecological restoration plants/crops |
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