CN106069739B - Method for simply, quickly and high-flux screening brassica plants with low heavy metal cadmium accumulation - Google Patents
Method for simply, quickly and high-flux screening brassica plants with low heavy metal cadmium accumulation Download PDFInfo
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Abstract
The invention discloses a simple, rapid and high-throughput screening method for brassica plants with low accumulation of heavy metal cadmium, and relates to the technical field of breeding of brassica plants and germplasm resource screening. The method comprises the following steps: firstly, disinfecting seeds; preparing a seed germination bed; placing seeds; fourthly, accelerating germination; fifth, sprouting growth; sixthly, measuring the length of the radicle; seventhly, evaluating the inhibition effect of the radicle length in the cotyledon period; screening out Brassica plants which are low in heavy metal cadmium accumulation in the test group. The method can screen out the brassica plant with low accumulation of heavy metal cadmium only by treating the tested brassica plant seeds with the cadmium solution with a certain concentration on the seed germination bed, and has the advantages of simplicity and labor saving; the screening only needs one week, so that the screening period is greatly shortened, and the screening process is accelerated; the brassica plants with low accumulation of heavy metal cadmium in a large population can be screened out in a high-throughput manner; does not depend on expensive measuring instruments, has low cost and is easy to popularize and apply in a large range.
Description
Technical Field
The invention relates to the technical field of breeding of brassica plants and germplasm resource screening, in particular to a simple, rapid and high-throughput screening method of brassica plants with low heavy metal cadmium accumulation, which is used for screening excellent brassica plant germplasm materials with low heavy metal cadmium accumulation.
Background
Cadmium (Cadmium, Cd) is located in group IIB of the fifth period of the periodic table, has a molecular weight of 112.41, is a silvery white shiny metal, and is widely used in the chemical industry. The heavy metal cadmium is a nonessential metal element of organisms and is one of heavy metal elements which have toxic action on human, animals and plants; the high concentration of heavy metal cadmium can inhibit the growth and development of plants, so that the yield of crops is reduced; in addition, the heavy metal cadmium accumulated in crops can be enriched in human bodies through food chains, and poses serious threats to the health of human beings.
With the development of industrialization and urbanization, many countries in the world face a serious cadmium pollution problem. In the 50 to 70 th ages of the 20 th century, a public disease- 'osteodynia' occurs in Shentong Chuanyun basin in Fushan county of Japan due to serious cadmium pollution; since then, the problem of cadmium contamination, a heavy metal, has attracted widespread attention throughout the world. In China, the ministry of environmental protection, the statistical bureau and the ministry of agriculture in 2 months in 2010 jointly release a first national pollution source census bulletin, wherein data show that the total emission amount of heavy metal pollutants (cadmium, chromium, arsenic, mercury and lead) in China is up to 0.09 million tons. The national soil pollution condition survey bulletin issued in 4 months in 2014 shows that the total point exceeding rate of the national soil is 16.1 percent, the point exceeding rate of heavy metal cadmium reaches 7.0 percent, and the first of pollutants are located; in addition, the overproof rate of the soil point position of the cultivated land is the highest and reaches 19.4 percent, so that the pollution of heavy metal cadmium seriously threatens the agricultural production in most areas of China.
Brassica napus in brassica plants is a main oil crop in China, Chinese cabbages, pakchoi, cabbages, broccoli and the like in brassica plants are main daily consumption vegetables, and reports report that the pollution of heavy metal cadmium in main production areas such as the Hunan and the Jiangxi is serious, so that the safe production of the brassica plants is greatly threatened. Researches show that the heavy metal cadmium pollution can inhibit the growth and development of brassica plants, so that the symptoms of development delay, poor growth and the like are caused, and the yield of the brassica plants is finally reduced. Researches prove that the brassica plants are easy to enrich heavy metal cadmium in soil, the cadmium is easy to migrate to edible organs, the quality of the brassica plants can be seriously reduced, the produced agricultural products do not meet the safety standard, and the brassica plants can be greatly damaged by eating the agricultural products by mistake.
The enrichment effect of plants on heavy metal cadmium not only has interspecific difference, but also has obvious difference in the seeds. Researches related to screening of crop germplasm resources with low accumulation of heavy metal cadmium and breeding of new varieties become a hot spot of current domestic and foreign researches; the results of previous researches show that the accumulation of heavy metal cadmium in crops such as rice, wheat and barley has large intraspecies variation. Screening and cultivating brassica plant materials with low accumulation of heavy metal cadmium, ensuring the safe production of brassica plants which are important economic crop systems, and being an important task of screening and breeding brassica plant germplasm resources. Therefore, an effective way is urgently needed to be explored, excellent resources with low accumulation of heavy metal cadmium are screened and excavated from brassica plant germplasm resources, and the cultivation of new brassica plant varieties with low accumulation of heavy metal cadmium is vigorously carried out.
The traditional method for screening the accumulation amount of heavy metal cadmium in brassica plants mainly uses instruments such as a graphite furnace atomic absorption spectrum instrument, a flame atomic absorption spectrum instrument, an inductively coupled plasma emission spectrum instrument and the like to measure the cadmium content, and the traditional method has the advantage of high precision; however, the detection cost is high, the instrument operation and the sample preparation are extremely complicated, and the large-scale screening of breeding populations and germplasm resource materials is difficult. Therefore, the method for simply, quickly and high-flux screening of the brassica plants with low accumulation of heavy metal cadmium is established, so that the screening and excavation work of excellent germplasm resources of the brassica plants with low accumulation can be accelerated, the early screening efficiency of the brassica plants with low accumulation in a breeding population can be improved, and the method plays a vital role in promoting the safe production work of the brassica plants in areas polluted by heavy metal cadmium.
Disclosure of Invention
The invention aims to overcome the defects and shortcomings of the prior art and provide a simple, rapid and high-throughput screening method for brassica plants with low accumulation of heavy metal cadmium.
The method for screening the brassica plant with low accumulation of heavy metal cadmium provided by the invention comprises the following steps:
disinfecting seeds
Selecting a plurality of test groups of full, disease and insect free and uniform-sized brassica plant seeds, wherein 300 seeds in each group are sterilized by 75% alcohol for 5min and washed clean by distilled water, and after the sterilization is finished, absorbing water on the surfaces of the seeds by filter paper for later use;
② preparation of seed germinating bed
The seed germination bed is divided into a treatment group seed germination bed and a control group seed germination bed:
spreading 4 layers of qualitative filter paper in a culture dish (square plastic dish with side length of 12 cm), and adding Cd with concentration of 1-50mg/L2+15mL of solution, namely the seed germination bed of the treatment group;
spreading 4 layers of qualitative filter paper in a culture dish (a square plastic dish with the side length of 12 cm), and adding equivalent double distilled water to obtain a control group seed germination bed;
each treatment group and control group were each provided with 3 replicates;
③ placing seeds
Uniformly placing the seeds sterilized in the step I on the germination bed in the step II, wherein the germination density is that 50 seeds are placed in each dish, and sealing the culture dish by using a sealing film;
germination accelerating treatment
Placing the sealed culture dish in the step III in an artificial climate box, culturing for 2 days in the dark at 22 ℃, and performing germination accelerating treatment;
fifth germination and growth
After the germination is finished, placing the seeds in a 22 ℃ artificial climate box, and culturing for 5 days under the conditions of 16h of light and 8h of darkness;
measurement of radicle Length
Selecting 10 seedlings with consistent growth from each dish, and measuring the radicle length of the seedlings;
seventhly, evaluation of inhibitory action on cotyledon stage radicle length
Evaluating the inhibition effect of the radicle length of the cotyledon period under the cadmium stress of each reference group according to the root length of the control group and the treatment group measured in the step (sixthly);
screening out Brassica plants with low heavy metal cadmium accumulation in test groups
Selecting the brassica plant with small suppression effect on the length of radicle in cotyledon period under cadmium stress in the reference group, namely the brassica plant with low accumulation of heavy metal cadmium.
The radicle length in the step (sixthly) is the whole length of the young seedling from the root tip to the connecting point of the radicle and the hypocotyl in the cotyledon stage of the brassica plant, and the unit is millimeter.
The evaluation indexes of the inhibition effect of the cotyledon stage Radicle Length under Cadmium stress in the step (c) comprise Cadmium damage Coefficient (CTI) and Relative Radicle Length (RRL);
wherein: cadmium damage coefficient CTI = (RL _ ck-RL _ Cd)/RL _ ck multiplied by 100;
relative radicle length RRL = RL _ Cd/RL _ ck;
RL is the length of the radicle,
RL _ ck is the radicle length of the control group,
RL _ Cd is the length of the radicle of the cadmium treated group.
The basis for screening out brassica plants with low accumulation of heavy metal cadmium in the test group is as follows: the smaller the radicle length inhibition effect of the brassica seed in the cotyledon period under the cadmium stress, the less the accumulation amount of heavy metal cadmium in the material, and the larger the radicle length inhibition effect, the more the accumulation amount of heavy metal cadmium in the material.
The invention has the following advantages and positive effects:
firstly, only needing to treat tested brassica seeds with a cadmium solution with a certain concentration on a seed germination bed, screening brassica plants with low accumulation of heavy metal cadmium, and having the advantages of simplicity and labor saving compared with the traditional methods of water culture, soil culture, field culture and the like;
the screening only needs one week, the screening period is greatly shortened, and the screening process is accelerated;
thirdly, brassica plants with low heavy metal cadmium accumulation in large groups can be screened out in a high-throughput manner;
and fourthly, the method does not depend on expensive measuring instruments, has low cost and is easy to popularize and apply in a large range.
Drawings
FIG. 1 is a schematic diagram of a root length measurement method;
wherein A: the linking point of radicle and hypocotyl; b: root tip; c: root length.
FIG. 2 is a schematic representation of the cadmium content in cotyledons of different brassica napus varieties after treatment with a cadmium solution.
The abscissa of the graph is a cabbage type rape variety, and the ordinate is the cadmium content (mg/Kg) in the cabbage type rape cotyledon;
A1-A4 are 4 cabbage type rape varieties with the length of radicle at the cotyledon stage being little inhibited by cadmium (low accumulation of cadmium);
B1-B4 are 4 cabbage type rape varieties with the length of the radicle at the cotyledon stage greatly inhibited by cadmium (high accumulation of cadmium).
FIG. 3 is a schematic diagram of the cadmium content in cotyledons of different cabbage varieties after treatment with a cadmium solution.
The abscissa of the graph is the Chinese cabbage variety, and the ordinate is the cadmium content (mg/Kg) in the Chinese cabbage cotyledon;
A1-A4 is 4 Chinese cabbage varieties with the length of radicle in cotyledon period being less inhibited by cadmium (low accumulation of cadmium);
B1-B4 are 4 Chinese cabbage varieties with the length of radicle in cotyledon period greatly inhibited by cadmium (high accumulation of cadmium).
The specific implementation mode is as follows:
the following detailed description is made with reference to the accompanying drawings and examples:
example 1:
to measure 8 Brassica napus varieties in control (double distilled water treatment) and 50mg/L Cd2+Evaluating the inhibition effect of the cotyledon-stage radicle length of the brassica napus under the stress of cadmium solution by taking the cadmium damage coefficient as an index on the cotyledon-stage radicle length of the brassica napus after the solution treatment, grading the inhibition effect, screening out the varieties with low accumulation of heavy metal cadmium in the brassica napus varieties, and explaining the specific implementation mode of the invention; and then measuring the cadmium accumulation amount in the cotyledon of the tested cabbage type rape seed after the cadmium stress, and statistically analyzing the accumulation rule of heavy metal cadmium in the cabbage type rape seed leaves of different grades to verify the screening result of the invention.
1. The specific embodiment for screening the low-accumulation cadmium cabbage type rape variety is as follows:
1) seed disinfection
Selecting 300 seeds which are full, free of diseases and insects and uniform in size of the cabbage type rape variety to be screened, disinfecting the seeds with 75% alcohol for 5min, and washing the seeds with distilled water; after the disinfection is finished, absorbing the water on the surface of the seeds by using filter paper for standby;
2) preparation of seed germinating bed
Spreading 4 layers of qualitative filter paper in a culture dish (square plastic dish with side length of 12 cm), adding 50mg/L Cd into the treatment group2+15mL of solution, and 3 replicates of each treatment setup with the same amount of double distilled water in the control group;
3) placing seeds
Uniformly placing the seeds sterilized in the step 1) on the germination bed in the step 2), wherein the germination density is that 50 seeds are placed in each dish, and sealing the culture dish by using a sealing film;
4) pregermination treatment
Placing the sealed culture dish in the step 3) in an artificial climate box for dark culture at 22 ℃ for 2 days, and carrying out germination accelerating treatment;
5) growth of sprouts
After the germination is finished, placing the seeds in a 22 ℃ artificial climate box, and culturing for 5 days under the conditions of 16h of illumination and 8h of darkness;
6) measurement of the Length of cotyledonary stage radicles
Selecting 10 seedlings with consistent growth from each dish, and measuring the radicle length of the seedlings;
7) evaluation of inhibitory Effect on cotyledon stage radicle Length
Calculating cadmium damage coefficients [ CTI = (RL _ ck-RL _ Cd)/RL _ ck x 100 of each variety by using Excel 2003, wherein RL is the radicle length, RL _ ck is the radicle length of a control group, and RL _ Cd is the radicle length of a cadmium treatment group), and grading the inhibition degree of the radicle length according to the cadmium damage coefficients, wherein the cadmium damage coefficients smaller than 50 are grades A and are numbered A1-A4, and the corresponding cadmium damage coefficients larger than 50 are grades B and are numbered B1-B4.
Note: only 8 varieties are tested in the test, so that the test is only divided into two stages, and if the population of the tested materials is large, a plurality of grades can be appropriately divided.
8) Screening out material for low-accumulation heavy metal cadmium in cabbage type rape to be tested
In the embodiment, the radicle inhibition effect of the four varieties A1-A4 is small, and the four varieties are screened cabbage type rape varieties with low accumulation of heavy metal cadmium.
2. The specific embodiment for verifying the screening results of the present invention is as follows:
1) sample collection
Treating cabbage type rape seedlings for one week under cadmium stress, washing with distilled water for more than 3 times, washing Cd2+ possibly remaining on the surfaces, sucking water on the surfaces of the seedlings, separating cotyledons of each seedling, and collecting the cotyledons in kraft paper bags;
2) sample drying process
After the collection is finished, the material is placed in a drying oven to be de-enzymed for 15 min at the temperature of 105 ℃, and then is dried in the drying oven for 48 h at the temperature of 60 ℃ until the weight is constant;
3) sample digestion
Crushing the completely dried sample, weighing 0.3-0.5G (marked as G) in a polytetrafluoroethylene digestion tube, and adding 5mL of mixed acid (HNO 3: HClO4=4: 1) for overnight (12 h) digestion;
4) sample digestion
The digested sample is put on an electric hot plate for cooking, and the temperature is gradually increased: digesting at 60 ℃, 30 min, 120 ℃, 30 min, 160 ℃, 1 h and 200 ℃ until the solution is transparent and clear;
5) filtration constant volume
The solution obtained by digestion is filtered into a volumetric flask of 5mL through a funnel, and the volume is fixed by double distilled water for standby.
6) Determination of cadmium content
Detecting the content of metal cadmium (marked as Cl) in the sample after constant volume by adopting an inductively coupled plasma emission spectrum (instrument model: a thermoelectric intRed XSP plasma atomic emission spectrometer);
7) calculation of cadmium content in tissue samples
Calculating the cadmium content C of each variety in cotyledons treated by 50mg/L of Cd2+ solution, wherein the calculation formula is as follows: c = Cl 5/G. The average values of the cadmium content in the cabbage type rape cotyledons of the A-level (the cadmium damage coefficient is less than 50) and the B-level (the cadmium damage coefficient is more than 50) are 163.08 and 404.52 mg/Kg respectively;
8) statistical analysis of data
And (3) statistically analyzing the rule of the cadmium content in the brassica napus leaves of the A-grade and the B-grade (see figure 2), and obtaining a conclusion according to the statistical analysis result: the amount of cadmium accumulated by the cabbage type rape variety (A1-A4) with small cadmium damage coefficient (grade A and small radicle length inhibition) is obviously lower than that of the cabbage type rape variety (B1-B4) with large cadmium damage coefficient (grade B and large radicle length inhibition).
Therefore, the screening method is feasible, and can simply, quickly and accurately screen out the material with low accumulation of heavy metal cadmium in the brassica napus.
Example 2:
to measure 8 cabbage varieties in contrast (double distilled water treatment) and 30mg/L Cd2+Evaluating the inhibition effect of the cotyledon period radicle length of the reference cabbage subjected to the cadmium solution stress by using the cadmium damage coefficient as an index, grading the inhibition effect, screening out the varieties with low heavy metal cadmium accumulation in the reference cabbage varieties, and explaining the specific embodiment of the invention; and then measuring the accumulation amount of cadmium in the cotyledon of the tested cabbage seed after the cadmium stress, and statistically analyzing the accumulation rules of the heavy metal cadmium in the cabbage leaves of different grades to verify the screening result of the invention.
1. The specific embodiment for screening the low-accumulation cadmium Chinese cabbage variety is as follows:
1) seed disinfection
Selecting 300 full, disease and insect free and uniform seeds of the Chinese cabbage variety to be screened, sterilizing with 75% alcohol for 5min, and washing with distilled water; after the disinfection is finished, absorbing the water on the surface of the seeds by using filter paper for standby;
2) preparation of seed germinating bed
Spreading 4 layers of qualitative filter paper in a culture dish (square plastic dish with side length of 12 cm), adding 50mg/L Cd into the treatment group2+15mL of solution, and 3 replicates of each treatment setup with the same amount of double distilled water in the control group;
3) placing seeds
Uniformly placing the seeds sterilized in the step 1) on the germination bed in the step 2), wherein the germination density is that 50 seeds are placed in each dish, and sealing the culture dish by using a sealing film;
4) pregermination treatment
Placing the sealed culture dish in the step 3) in an artificial climate box for dark culture at 22 ℃ for 2 days, and carrying out germination accelerating treatment;
5) growth of sprouts
After the germination is finished, placing the seeds in a 22 ℃ artificial climate box, and culturing for 5 days under the conditions of 16h of illumination and 8h of darkness;
6) measurement of the Length of cotyledonary stage radicles
Selecting 10 seedlings with consistent growth from each dish, and measuring the radicle length of the seedlings;
7) evaluation of inhibitory Effect on cotyledon stage radicle Length
Calculating cadmium damage coefficients [ CTI = (RL _ ck-RL _ Cd)/RL _ ck x 100 of each variety by using Excel 2003, wherein RL is the radicle length, RL _ ck is the radicle length of a control group, and RL _ Cd is the radicle length of a cadmium treatment group), and grading the inhibition degree of the radicle length according to the cadmium damage coefficients, wherein the cadmium damage coefficients smaller than 50 are grades A and are numbered A1-A4, and the corresponding cadmium damage coefficients larger than 50 are grades B and are numbered B1-B4.
Note: only 8 varieties are tested in the test, so that the test is only divided into two stages, and if the population of the tested materials is large, a plurality of grades can be appropriately divided.
8) Screening out material for low-accumulation heavy metal cadmium in ginseng test Chinese cabbage
In the embodiment, the embryonic root inhibition effects of the four varieties A1-A4 are small, and the four varieties are selected as the cabbage varieties with low accumulation of heavy metal cadmium.
2. The specific embodiment for verifying the screening results of the present invention is as follows:
1) sample collection
Treating cabbage seedlings for one week under cadmium stress, washing with distilled water for more than 3 times, washing Cd2+ possibly remaining on the surface, sucking off water on the surfaces of the seedlings, separating cotyledons of each seedling, and collecting in kraft paper bags;
2) sample drying process
After the collection is finished, the material is placed in a drying oven to be de-enzymed for 15 min at the temperature of 105 ℃, and then is dried in the drying oven for 48 h at the temperature of 60 ℃ until the weight is constant;
3) sample digestion
Crushing the completely dried sample, weighing 0.3-0.5G (marked as G) in a polytetrafluoroethylene digestion tube, and adding 5mL of mixed acid (HNO 3: HClO4=4: 1) for overnight (12 h) digestion;
4) sample digestion
The digested sample is put on an electric hot plate for cooking, and the temperature is gradually increased: digesting at 60 ℃, 30 min, 120 ℃, 30 min, 160 ℃, 1 h and 200 ℃ until the solution is transparent and clear;
5) filtration constant volume
The solution obtained by digestion is filtered into a volumetric flask of 5mL through a funnel, and the volume is fixed by double distilled water for standby.
6) Determination of cadmium content
Detecting the content of metal cadmium (marked as Cl) in the sample after constant volume by adopting an inductively coupled plasma emission spectrum (instrument model: a thermoelectric intRed XSP plasma atomic emission spectrometer);
7) calculation of cadmium content in tissue samples
Calculating the cadmium content C of each variety in cotyledons treated by 30mg/L Cd2+ solution, wherein the calculation formula is as follows: c = Cl 5/G. The average values of the cadmium content in the cabbage cotyledon of the A-grade (the cadmium damage coefficient is less than 50) and the B-grade (the cadmium damage coefficient is more than 50) are 315.35 and 505.38 mg/Kg respectively;
8) statistical analysis of data
The rule of cadmium content in the cabbage leaves of the A level and the B level (see figure 2) is statistically analyzed, and a conclusion can be obtained according to the statistical analysis result: the amount of cadmium accumulated by the Chinese cabbage variety (A1-A4) with small cadmium damage coefficient (grade A and small radicle length inhibition) is obviously lower than that of the Chinese cabbage variety (B1-B4) with large cadmium damage coefficient (grade B and large radicle length inhibition).
Therefore, the screening method is feasible, and can simply, quickly and accurately screen out the materials with low heavy metal cadmium accumulation in the reference Chinese cabbage.
Claims (1)
1. A simple, rapid and high-throughput screening method for brassica plants with low accumulation of heavy metal cadmium is characterized by comprising the following steps:
disinfecting seeds
Selecting a plurality of test groups of full, disease and insect free and uniform-sized brassica plant seeds, wherein 300 seeds in each group are sterilized by 75% alcohol for 5min and washed clean by distilled water, and after the sterilization is finished, absorbing water on the surfaces of the seeds by filter paper for later use; the brassica plants are cabbage type rape and Chinese cabbage;
② preparation of seed germinating bed
The seed germination bed is divided into a treatment group seed germination bed and a control group seed germination bed:
spreading 4 layers of qualitative filter paper in a culture dish (square plastic dish with side length of 12 cm), and adding Cd with concentration of 1-50mg/L2+15mL of solution, namely the seed germination bed of the treatment group;
spreading 4 layers of qualitative filter paper in a culture dish, namely a square plastic dish with the side length of 12cm, and adding equivalent double distilled water, namely the control group seed germination bed;
each treatment group and control group were each provided with 3 replicates;
③ placing seeds
Uniformly placing the seeds sterilized in the step I on the germination bed in the step II, wherein the germination density is that 50 seeds are placed in each dish, and sealing the culture dish by using a sealing film;
germination accelerating treatment
Placing the sealed culture dish in the step III in an artificial climate box, culturing for 2 days in the dark at 22 ℃, and performing germination accelerating treatment;
fifth germination and growth
After the germination is finished, placing the seeds in a 22 ℃ artificial climate box, and culturing for 5 days under the conditions of 16h of illumination and 8h of darkness;
measurement of radicle Length
Selecting 10 seedlings with consistent growth from each dish, and measuring the radicle length of the seedlings;
seventhly, evaluation of inhibitory action on cotyledon stage radicle length
Evaluating the inhibition effect of the radicle length of the cotyledon period under the cadmium stress of each reference group according to the root length of the control group and the treatment group measured in the step (sixthly);
screening out Brassica plants with low heavy metal cadmium accumulation in test groups
Selecting the brassica plant with small suppression effect on the length of radicle in cotyledon period under cadmium stress in the reference group, namely the brassica plant with low accumulation of heavy metal cadmium;
the radicle length in the step (sixthly) is the whole length of the young seedling from the root tip to the connection point of the radicle and the hypocotyl in the cotyledon stage of the brassica plant, and the unit is millimeter;
the evaluation indexes of the inhibition effect of the radicle length of the cotyledon period under the cadmium stress in the step (c) comprise a cadmium damage coefficient CTI and a relative radicle length RRL;
wherein: the cadmium damage coefficient CTI is (RL _ ck-RL _ Cd)/RL _ ck multiplied by 100;
relative radicle length RRL-RL _ Cd/RL _ ck;
RL is the length of the radicle,
RL _ ck is the radicle length of the control group,
RL _ Cd is the length of the radicle of the cadmium treatment group;
the basis for screening out brassica plants with low accumulation of heavy metal cadmium in the test group is as follows: the smaller the radicle length inhibition effect of the brassica seed in the cotyledon period under the cadmium stress, the less the accumulation amount of heavy metal cadmium in the material, and the larger the radicle length inhibition effect, the more the accumulation amount of heavy metal cadmium in the material.
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| CN107130036B (en) * | 2017-05-31 | 2021-03-30 | 湖南科技大学 | Gene and method for quickly judging brassica plant with low cadmium accumulation |
| CN107262388A (en) * | 2017-08-03 | 2017-10-20 | 河北农业大学 | Method for rapidly screening low-accumulation heavy metal crop varieties and device used by same |
| CN107607700B (en) * | 2017-09-08 | 2019-06-18 | 中国农业科学院棉花研究所 | A kind of identification method of the resistance to cadmium of cotton |
| CN109618592A (en) * | 2018-12-03 | 2019-04-16 | 南京迪天高新产业技术研究院有限公司 | A method of screening the rice varieties of low accumulation toxic heavy metal |
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