CN107821132A - A kind of optimal water salt threshold value breeding method of Reed rhizome budding - Google Patents
A kind of optimal water salt threshold value breeding method of Reed rhizome budding Download PDFInfo
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- CN107821132A CN107821132A CN201711059927.2A CN201711059927A CN107821132A CN 107821132 A CN107821132 A CN 107821132A CN 201711059927 A CN201711059927 A CN 201711059927A CN 107821132 A CN107821132 A CN 107821132A
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- rhizome
- reed rhizome
- reed
- budding
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
A kind of optimal water salt threshold value breeding method of Reed rhizome budding, the present invention relates to a kind of optimal water salt threshold value breeding method of Reed rhizome budding.The related data of rhizome budding can not intuitively be reflected from envirment factor the present invention is to solve existing Propagation of Rhizomes method, it is impossible to the problem of providing the water salt scope that can plant reed with reconstruction for the recovery of field degeneration sabkha.The method of the present invention：1st, field Reed rhizome gathers；2nd, Reed rhizome is handled；3rd, cultivate and rhizome Budding process detects.The present invention is used for the cultivation of Reed rhizome.The present invention is easy to operate, cost-effective, and the recovery for being suitably for inland and beach plav provides seedling, there is important practice significance and application value.
Description
Technical field
The present invention relates to a kind of optimal water salt threshold value breeding method of Reed rhizome budding.
Background technology
Reed (Phragmites australis) is perennial rhizome type dogstail, cosmopolitan, in fresh water and salt water
Marsh can be distributed, and be one of the main marsh type in China.Reed has two kinds of breeding sides of seminal propagation and division propagation
Formula, but it is typical clonal line, and natural population relies primarily on supplement renewal of nourishing and generating.Reed crawl root-like stock hair
Reach, scope length in root area is deep also up to more than 1m below topsoil up to several meters.
Reed has extensive economic value：1st, the crude protein content of vegetative growth phase occupy first-class in dogstail, is one
The excellent feed of kind；2nd, the reed stalk in maturity period is hard, and quality is fine and smooth, fiber content height (40%~60%), is China's weight
The paper making raw material and handicraft raw material wanted；3rd, root is huger, can absorb heavy metal and other pollutants, has efficient
Water quality purification function；4th, plav is fish, shrimp, shellfish, crab settlement, is the place that various aquatic birds, birds inhabit, has and inhabit
The function on ground and abundant bio-diversity；5th, plav vegetation coverage, biomass is big, have swamp protection, carbon fixing and
Improve the important Ecology Actions such as salt-soda soil.
Very serious, relevant departments' just actively input substantial contribution recovery degeneration however, China's reed wetland is degenerated at present
Reed wetland, but relevant recovering technology is still very limited；Existing Propagation of Rhizomes method mostly enters from the angle of envirment factor
Hand improves germination rate, ignores the influence of biotic factor in itself.Therefore, consider quickly to breed reed in itself with full from rhizome
Sufficient industrial and agricultural production and environmental protection need, and have important practice significance and application value.
The content of the invention
The dependency number of rhizome budding can not intuitively be reflected from envirment factor the present invention is to solve existing Propagation of Rhizomes method
According to, it is impossible to the problem of providing the water salt scope that can plant reed with reconstruction for the recovery of field degeneration sabkha, and a kind of reed is provided
The optimal water salt threshold value breeding method of reed root stem budding.
A kind of optimal water salt threshold value breeding method of Reed rhizome budding of the present invention is to carry out according to the following steps：
First, field Reed rhizome gathers：In the plant roots and stems budding time of annual mid or late April, inland or beach are chosen
The uniform sampling point of growth of seedling in plav, by way of small patch takes rhizome, take to horizontal rhizome, it is optimal to dig
It is 20~30cm to take depth, after taking rhizome, is fitted into black plastic bag, avoids sunlight, and spray moisture；
2nd, Reed rhizome is handled：The Reed rhizome taken is rinsed, cuts aerial part, retains underground rhizome
As material is cultivated, health, a diameter of 4~7mm rhizome is therefrom chosen, the segment of 20cm length is cut into, ensures each segment
At least 2 nodes, obtain Reed rhizome to be cultivated；
3rd, cultivate and rhizome Budding process detects：Will Reed rhizome be cultivated temperature be 22 DEG C~28 DEG C under conditions of
It is placed in plastic tub and is cultivated by matrix of quartz sand, stromal thickness 5cm, salt solution is poured into plastic tub to no mistake stone
Sand surface, rhizome Budding process is monitored：Supplement water pours 500mL salt solution in every 2 weeks to ensure constant level daily,
When plumule elongation 2mm is to germinate, i.e. the optimal water salt threshold value of completion Reed rhizome budding is cultivated；The mass concentration of the salt solution is equal
For 5 ‰.
The beneficial effects of the invention are as follows：
This method carries out Reed rhizome budding using the salinity of the 0cm depth of waters 5 ‰ and cultivated, the results showed that, match somebody with somebody with other water salt
Than compared to the inventive method makes the budding time at least shorten 3 days, and bud ratio, survival rate and relative growth rate difference are at least
Improve 26%, 19%, 0.06cm/d.The efficiency that this method will improve degeneration salt marsh restoration with rebuild, and it is cost-effective.
Embodiment
Embodiment one：A kind of optimal water salt threshold value breeding method of Reed rhizome budding of present embodiment is by following
Step is carried out：
First, field Reed rhizome gathers：In the plant roots and stems budding time of annual mid or late April, inland or beach are chosen
The uniform sampling point of growth of seedling in plav, by way of small patch takes rhizome, take to horizontal rhizome, it is optimal to dig
It is 20~30cm to take depth, after taking rhizome, is fitted into black plastic bag, avoids sunlight, and spray moisture；
2nd, Reed rhizome is handled：The Reed rhizome taken is rinsed, cuts aerial part, retains underground rhizome
As material is cultivated, health, a diameter of 4~7mm rhizome is therefrom chosen, the segment of 20cm length is cut into, ensures each segment
At least 2 nodes, obtain Reed rhizome to be cultivated；
3rd, cultivate and rhizome Budding process detects：Will Reed rhizome be cultivated temperature be 22 DEG C~28 DEG C under conditions of
It is placed in plastic tub and is cultivated by matrix of quartz sand, stromal thickness 5cm, salt solution is poured into plastic tub to no mistake stone
Sand surface, rhizome Budding process is monitored：Supplement water pours 500mL salt solution in every 2 weeks to ensure constant level daily,
When plumule elongation 2mm is to germinate, i.e. the optimal water salt threshold value of completion Reed rhizome budding is cultivated；The mass concentration of the salt solution is equal
For 5 ‰.
It is to keep rhizome surface wettability that moisture is sprayed in present embodiment step 1.
Present embodiment ensures do not have moisture in matrix before rhizome to be transferred to plastic tub.
Embodiment two：Present embodiment is unlike embodiment one：Most preferably dug described in step 1
It is 25cm to take depth.It is other identical with embodiment one.
Embodiment three：Unlike one of present embodiment and embodiment one or two：Institute in step 2
Stating aerial part includes the aerial stem of plant, tender tip and blade position.It is other identical with embodiment one or two.
Embodiment four：Unlike one of present embodiment and embodiment one to three：Institute in step 3
The particle diameter for stating quartz sand is 1~2mm.It is other identical with embodiment one to three.
Embodiment five：Unlike one of present embodiment and embodiment one to four：Institute in step 3
State a length of 52cm of plastic tub, a width of 38cm, a height of 19cm.It is other identical with embodiment one to four.
Embodiment six：Unlike one of present embodiment and embodiment one to five：Institute in step 3
State to be placed under sunlight after quartz sand is rinsed well using running water and dry.It is other identical with embodiment one to five.
With reference to case study on implementation, the invention will be further described, but protection scope of the present invention is not limited to this.
Embodiment one：A kind of optimal water salt threshold value breeding method of Reed rhizome budding is to carry out according to the following steps：
First, field Reed rhizome gathers：In the plant roots and stems budding time of annual mid or late April, Mo Moge protection zones are chosen
Plav in the uniform sampling point of growth of seedling, by way of small patch takes rhizome, take to horizontal rhizome, most preferably
It is 20~30cm to take depth, after taking rhizome, is fitted into black plastic bag, avoids sunlight, and spray moisture；
2nd, Reed rhizome is handled：The Reed rhizome taken is rinsed, cuts aerial part, retains underground rhizome and makees
To cultivate material, health, a diameter of 4~7mm rhizome is therefrom chosen, the segment of 20cm length is cut into, ensures each segment extremely
Rare 2 nodes, and each individual is weighed, its weight is recorded, obtains Reed rhizome to be cultivated；
3rd, cultivate and rhizome Budding process detects：Will Reed rhizome be cultivated temperature be 22 DEG C~28 DEG C under conditions of
It is placed in plastic tub and is cultivated by matrix of quartz sand, stromal thickness 5cm pours into water into plastic tub, then to plastics
500mL salt solution is poured into basin；Multiple experimental groups are set, and equidistantly bury parallel with short side puts 12 rhizomes to each plastic tub, is inciting somebody to action
Rhizome ensures do not have moisture in matrix before being transferred to plastic tub；A length of 52cm of the plastic tub, a width of 38cm, a height of 19cm；
The depth of water gradient of cultivation is 0cm, 5cm, 10cm, and the wherein 0cm depth of waters there be not quartz sand surface just as standard using water layer, the salt
The mass concentration gradient of water is 0,2.5 ‰, 5 ‰, 7.5 ‰, 10 ‰, totally 15 groups of experiment conditions, and every group of experiment condition sets 3 examinations
Sample, each sample include 12 rhizomes, and experiment shares 3 × 5 × 3 × 12=540 sample size；Rhizome Budding process is supervised
Survey：Water is supplemented daily to ensure constant level, and the every 2 weeks salt solution that 500mL is poured in each sample, regularly statistics is germinateed daily
Number, using plumule elongation 2mm for germination, count within continuous 1 week no sprouting and occur being considered as experiment and terminate.
Embodiment two：A kind of optimal water salt threshold value breeding method of Reed rhizome budding is to carry out according to the following steps：
First, field Reed rhizome gathers：In the plant roots and stems budding time of annual mid or late April, choose to extra large protection zone
The uniform sampling point of growth of seedling in plav, by way of small patch takes rhizome, take to horizontal rhizome, it is optimal to dig
It is 20~30cm to take depth, after taking rhizome, is fitted into black plastic bag, avoids sunlight, and spray moisture；
2nd, Reed rhizome is handled：The Reed rhizome taken is rinsed, cuts aerial part, retains underground rhizome and makees
To cultivate material, health, a diameter of 4~7mm rhizome is therefrom chosen, the segment of 20cm length is cut into, ensures each segment extremely
Rare 2 nodes, and each individual is weighed, its weight is recorded, obtains Reed rhizome to be cultivated；
3rd, cultivate and rhizome Budding process detects：Will Reed rhizome be cultivated temperature be 22 DEG C~28 DEG C under conditions of
It is placed in plastic tub and is cultivated by matrix of quartz sand, stromal thickness 5cm pours into water into plastic tub, then to plastics
500mL salt solution is poured into basin；Multiple experimental groups are set, and equidistantly bury parallel with short side puts 12 rhizomes to each plastic tub, is inciting somebody to action
Rhizome ensures do not have moisture in matrix before being transferred to plastic tub；A length of 52cm of the plastic tub, a width of 38cm, a height of 19cm；
The depth of water gradient of cultivation is 0cm, 5cm, 10cm, and the wherein 0cm depth of waters there be not quartz sand surface just as standard using water layer, the salt
The mass concentration gradient of water is 0,2.5 ‰, 5 ‰, 7.5 ‰, 10 ‰, totally 15 groups of experiment conditions, and every group of experiment condition sets 3 examinations
Sample, each sample include 12 rhizomes, and experiment shares 3 × 5 × 3 × 12=540 sample size；Rhizome Budding process is supervised
Survey：Water is supplemented daily to ensure constant level, and the every 2 weeks salt solution that 500mL is poured in each sample, regularly statistics is germinateed daily
Number, using plumule elongation 2mm for germination, count within continuous 1 week no sprouting and occur being considered as experiment and terminate.
Embodiment three：A kind of optimal water salt threshold value breeding method of Reed rhizome budding is to carry out according to the following steps：
First, field Reed rhizome gathers：In the plant roots and stems budding time of annual mid or late April, choose OX-heart set and protect country
The uniform sampling point of growth of seedling in the plav of wetland park, by way of small patch takes rhizome, take to horizontal branch
At stem, it is 20~30cm most preferably to take depth, after taking rhizome, is fitted into black plastic bag, avoids sunlight, and spray water
Point；
2nd, Reed rhizome is handled：The Reed rhizome taken is rinsed, cuts aerial part, retains underground rhizome and makees
To cultivate material, health, a diameter of 4~7mm rhizome is therefrom chosen, the segment of 20cm length is cut into, ensures each segment extremely
Rare 2 nodes, and each individual is weighed, its weight is recorded, obtains Reed rhizome to be cultivated；
3rd, cultivate and rhizome Budding process detects：Will Reed rhizome be cultivated temperature be 22 DEG C~28 DEG C under conditions of
It is placed in plastic tub and is cultivated by matrix of quartz sand, stromal thickness 5cm pours into water into plastic tub, then to plastics
500mL salt solution is poured into basin；Multiple experimental groups are set, and equidistantly bury parallel with short side puts 12 rhizomes to each plastic tub, is inciting somebody to action
Rhizome ensures do not have moisture in matrix before being transferred to plastic tub；A length of 52cm of the plastic tub, a width of 38cm, a height of 19cm；
The depth of water gradient of cultivation is 0cm, 5cm, 10cm, and the wherein 0cm depth of waters there be not quartz sand surface just as standard using water layer, the salt
The mass concentration gradient of water is 0,2.5 ‰, 5 ‰, 7.5 ‰, 10 ‰, totally 15 groups of experiment conditions, and every group of experiment condition sets 3 examinations
Sample, each sample include 12 rhizomes, and experiment shares 3 × 5 × 3 × 12=540 sample size；Rhizome Budding process is supervised
Survey：Water is supplemented daily to ensure constant level, and the every 2 weeks salt solution that 500mL is poured in each sample, regularly statistics is germinateed daily
Number, using plumule elongation 2mm for germination, count within continuous 1 week no sprouting and occur being considered as experiment and terminate.
Effect detection
Budding time, bud ratio, survival rate and relative growth speed are to reflect the important indicator of plant germination.Tables 1 and 2
In summarize the Reed rhizome budding of technology described in embodiment one results, it can be seen that the bud ratio of 15 kinds of water salt proportioning exists
Between 11.0 ± 3.9% and 51.0 ± 7.1%, average bud ratio is 19.2 ± 1.4%, and survival rate is in 7.5 ± 4.7% and 41.8
Between ± 9.3%, average viability is 16.5 ± 1.6%, and the budding time between 4.6 ± 0.9 days and 14.7 ± 1.8 days, puts down
The time sprouted for 11.0 ± 0.6 days, relative growth speed is average between 0.06 ± 0.01cm/d and 0.16 ± 0.02cm/d
RGR is 0.08 ± 0.01cm/d.Statistical analysis shows that the 0cm depth of waters, 5 ‰ salinity rhizomes budding time are significantly lower than other water salt
Proportioning, at least shorten 3 days；And bud ratio, survival rate and relative growth speed are significantly higher than other water salt proportioning (P<0.05), divide
Do not improve 26% at least, 19%, 0.06cm/d.The optimal water salt threshold value it can be seen that the Reed rhizome of the 0cm depth of waters, 5 ‰ salinities sprouts
Breeding method is significant effect.
1 15 kinds of water salt proportionings of table and the budding index of measurement
The variance analysis of each budding index under 2 15 kinds of water salt conditions of mixture ratios of table
| Budding index | Df | F | P |
| Bud ratio | 14 | 3.708 | <0.001 |
| Survival rate | 14 | 1.863 | <0.05 |
| Averagely sprout the time | 14 | 2.131 | <0.05 |
| Relative growth speed | 14 | 2.404 | <0.01 |
Claims (6)
- A kind of optimal water salt threshold value breeding method 1. Reed rhizome sprouts, it is characterised in that the optimal water salt threshold value of Reed rhizome budding Breeding method is to carry out according to the following steps：First, field Reed rhizome gathers：In the plant roots and stems budding time of annual mid or late April, the reed at selection inland or beach The uniform sampling point of growth of seedling in marsh, by way of small patch takes rhizome, take to horizontal rhizome, most preferably take depth Spend for 20~30cm, after taking rhizome, be fitted into black plastic bag, avoid sunlight, and spray moisture；2nd, Reed rhizome is handled：The Reed rhizome taken is rinsed, cuts aerial part, retains underground rhizome as training Material is educated, health, a diameter of 4~7mm rhizome is therefrom chosen, is cut into the segment of 20cm length, ensures each segment at least 2 Individual node, obtain Reed rhizome to be cultivated；3rd, cultivate and rhizome Budding process detects：Reed rhizome to be cultivated is placed under conditions of temperature is 22 DEG C~28 DEG C Cultivated in plastic tub and by matrix of quartz sand, stromal thickness 5cm, salt solution is poured into plastic tub to no mistake quartz sand Surface, rhizome Budding process is monitored：Supplement water pours 500mL salt solution in every 2 weeks, works as embryo to ensure constant level daily Bud elongation 2mm is to germinate, that is, completes the optimal water salt threshold value of Reed rhizome budding and cultivate；The mass concentration of the salt solution is 5‰。
- The optimal water salt threshold value breeding method 2. a kind of Reed rhizome according to claim 1 sprouts, it is characterised in that step It is 25cm most preferably to take depth described in one.
- The optimal water salt threshold value breeding method 3. a kind of Reed rhizome according to claim 1 sprouts, it is characterised in that step Aerial part described in two includes the aerial stem of plant, tender tip and blade position.
- The optimal water salt threshold value breeding method 4. a kind of Reed rhizome according to claim 1 sprouts, it is characterised in that step The particle diameter of quartz sand described in three is 1~2mm.
- The optimal water salt threshold value breeding method 5. a kind of Reed rhizome according to claim 1 sprouts, it is characterised in that step A length of 52cm of plastic tub described in three, a width of 38cm, a height of 19cm.
- The optimal water salt threshold value breeding method 6. a kind of Reed rhizome according to claim 1 sprouts, it is characterised in that step Quartz sand described in three is placed under sunlight after being rinsed well using running water and dried.
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Cited By (2)
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| CN109105210A (en) * | 2018-07-16 | 2019-01-01 | 中国科学院东北地理与农业生态研究所 | A kind of optimal medium matter screening technique of suitable reed budding |
| CN109122067A (en) * | 2018-07-16 | 2019-01-04 | 中国科学院东北地理与农业生态研究所 | A kind of screening technique of the best salt nitrogen condition of reed seedling |
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Cited By (2)
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| CN109105210A (en) * | 2018-07-16 | 2019-01-01 | 中国科学院东北地理与农业生态研究所 | A kind of optimal medium matter screening technique of suitable reed budding |
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Application publication date: 20180323 |