CN107232249A - A kind of medicine and preparation method for preventing and treating grapevine anthracnose - Google Patents
A kind of medicine and preparation method for preventing and treating grapevine anthracnose Download PDFInfo
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- CN107232249A CN107232249A CN201710586931.8A CN201710586931A CN107232249A CN 107232249 A CN107232249 A CN 107232249A CN 201710586931 A CN201710586931 A CN 201710586931A CN 107232249 A CN107232249 A CN 107232249A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/46—Stemonaceae [Stemona family], e.g. croomia
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Abstract
The invention provides a kind of medicine and preparation method for preventing and treating grapevine anthracnose, the insecticide includes following component：Artemisia spharocephala, car mulberry son, cordate houttuynia, delavay pararuellia herb, fiveleaf carpetweet herb, green bristlegrass, neem bark, Radix Isatidis, calamus, Golden Larch Bark, Herba Andrographitis, the tuber of stemona.Preparation method is：First to each component pulverization process, crushed mixture is then subjected to extraction operation, extraction is concentrated the liquid obtained after extraction after terminating, and prepares traditional Chinese medicine extraction pulvis, that is, obtains the medicine.Medicinal application of the present invention can not only effectively prevent and treat the infringement of anthrachose of grape during wine-growing, also long with efficacy time, the characteristics of safety non-pollution, and effect improves grape quality significantly, with preferable application value.
Description
Technical field
The present invention relates to application technical field, in particular it relates to a kind of medicine for preventing and treating grapevine anthracnose and preparation
Method.
Background technology
Grape, is one of most ancient distribution most wide a kind of fruit in the world, its berry be generally it is circular or oval, color and luster with
Kind and it is different.Grape nutritive value is very high, can be made into grape juice, raisins and grape wine.Grain is big, skin depth, juice are few, water is more, skin
Meat is easily separated, taste sweet and sour taste, and having outside pulp outside a layer thin skin, skin has Bao Shuan, and some kinds are without seed.Grape can both be cooked fruit life
Food, can also make wine or Making Raisins.
Grapevine anthracnose also known as shot hole, bird's eye disease, are one of important diseases of grape.This disease distribution is wide, occurs generally, I
Almost there is generation all viny regions of state.Fallen ill in rainy humid area most heavy.Anthrachose of grape often results in grape young sprout and leaf
Piece is withered, and fruit quality is deteriorated, and yield declines, and loses very big.
The green young tender part of anthrachose of grape main harm grape, such as fruit, carpopodium, cob, blade, vein, petiole, branch
Climing, young sprout and tendril etc., wherein based on fruit, blade, young sprout, fruit ear damage loss is maximum.Green children's okra fruit grain is aggrieved, just
There is auburn circular stigma in phase fruit face, expands later, formed diameter up to 3-8mm, edge puce, central canescence and
Slightly concave sunken scab, is similar to " bird's eye " shape.Later stage scab usually hardens, is cracked, and fruit diminishes, sour, loses edibility.Disease
Spot is only limitted to epidermis, does not go deep into pulp.Occur black dot when moist, on scab and overflow canescence mucus.The fruit later stage by
Evil is normally opened to split deformity.Ripe fruit is aggrieved, only occurs suberification spot on pericarp surface, influences quality.
Therefore, research and development are a kind of can effectively prevent and treat the medicine of grapevine anthracnose, with preferable application value, also accord with
Close the requirement of current wine-growing industry.
The content of the invention
For defect of the prior art, it is an object of the invention to provide a kind of medicine for preventing and treating grapevine anthracnose and preparation
Method, the medicinal application can not only effectively prevent and treat the infringement of anthrachose of grape, also with efficacy time during wine-growing
The characteristics of length, safety non-pollution, significantly effect, improve grape quality, with preferable application value.
The present invention solves technical problem and adopted the following technical scheme that：
The present invention relates to a kind of medicine for preventing and treating grapevine anthracnose, include each component of following parts by weight：Artemisia spharocephala 10 ~ 20
Part, 8 ~ 17 parts of car mulberry son, 15 ~ 22 parts of cordate houttuynia, 13 ~ 21 parts of delavay pararuellia herb, 6 ~ 11 parts of fiveleaf carpetweet herb, 16 ~ 24 parts of green bristlegrass, neem bark
10 ~ 18 parts, 12 ~ 20 parts of Radix Isatidis, 7 ~ 15 parts of calamus, 6 ~ 10 parts of Golden Larch Bark, 5 ~ 11 parts of Herba Andrographitis, 5 ~ 8 parts of the tuber of stemona.
Preferably, 13 parts of the Artemisia spharocephala, 12 parts of car mulberry son, 19 parts of cordate houttuynia, 16 parts of delavay pararuellia herb, 8 parts of fiveleaf carpetweet herb, dog tail
20 parts of grass, 13 parts of neem bark, 18 parts of Radix Isatidis, 9 parts of calamus, 8 parts of Golden Larch Bark, 6 parts of Herba Andrographitis, 7 parts of the tuber of stemona.
Preferably, 18 parts of the Artemisia spharocephala, 15 parts of car mulberry son, 16 parts of cordate houttuynia, 15 parts of delavay pararuellia herb, 10 parts of fiveleaf carpetweet herb, dog
18 parts of tail grass, 15 parts of neem bark, 15 parts of Radix Isatidis, 12 parts of calamus, 7 parts of Golden Larch Bark, 9 parts of Herba Andrographitis, 6 parts of the tuber of stemona.
The invention further relates to a kind of method for preparing the medicine for preventing and treating grapevine anthracnose, comprise the following steps：
（1）Each composition is weighed according to proportioning, is crushed respectively with pulverizer, and is well mixed, mixed powder is obtained；
（2）Mixed powder is placed in apparatus,Soxhlet's, under the conditions of 75 ~ 80 DEG C, flowed back in the in the mixed solvent of ethanol and water
4 ~ 6h is extracted, separation respectively obtains solid extract and extract solution；
（3）Then the solid extract after separation is placed in flask, 1 ~ 2h of the refluxing extraction that adds water, filters, obtain filtrate and filter
Slag, washs filter residue, merging filtrate and washing lotion, obtains extracting mixed liquor；
（4）By step（2）Obtained extract solution and step（3）Obtained extraction mixed liquor merges, and concentration, spray-on process is prepared
Drug powder.
Preferably, the step（1）Particle diameter after middle crushing is 80 ~ 150 mesh.
Preferably, the step（2）The ethanol of middle addition and the mixed solution quality of water are the 10 ~ 20 of mixed powder quality
Times, the volume ratio of ethanol and water is 1:0.5~2.
Preferably, the step（3）The amount of middle addition water is 10 ~ 20 times of mixed powder quality.
Preferably, the step（3）It is middle washing filter residue method be：Filter residue is placed in beaker, filter residue quality 3 ~ 8 is added
The water of times quality, 5 ~ 10min of ultrasonic agitation in ultrasonic wave, filtering, you can.
Preferably, the step（4）Middle use Rotary Evaporators are concentrated to mixed liquor, and mixed liquor is concentrated into original
3 ~ 5 times of mixed powder quality.
Compared with prior art, the present invention has following beneficial effect：
（1）The medicine of the present invention for preventing and treating grapevine anthracnose, the medicinal application can not only have during wine-growing
Effect prevents and treats the infringement of anthrachose of grape, and effect improves grape quality significantly, also long with efficacy time, the characteristics of safety non-pollution, keeps away
Exempt from because grape eats unsafe phenomenon caused by medicament residue, with preferable application value.
（2）The medicine of the present invention for preventing and treating grapevine anthracnose uses pure Chinese medicine component, not only with longer drug effect
Endurance, effectively prevents the generation of anthrachose of grape during grape growth, and the Chinese medicine does not result in injury to human body, with compared with
Good safety in utilization and edible safety, in addition, the medicine uses pulvis, carry with good, the characteristics of easy to use, make
Medicinal powder can be directly applied as needed with process or added water to being sprayed after hook, improve and use flexibility.
（3）The preparation method of the medicine of the present invention for preventing and treating grapevine anthracnose is simple and convenient to operate, process conditions temperature
With suitable industrialization promotion production.
Embodiment
With reference to specific embodiment, the present invention is expanded on further.These embodiments be merely to illustrate the present invention and without
In limitation the scope of the present invention.
Embodiment 1：
The present embodiment is related to a kind of medicine and preparation method for preventing and treating grapevine anthracnose；
The medicine is prepared from by following component：
13 parts of Artemisia spharocephala, 12 parts of car mulberry son, 19 parts of cordate houttuynia, 16 parts of delavay pararuellia herb, 8 parts of fiveleaf carpetweet herb, 20 parts of green bristlegrass, neem bark 13
Part, 18 parts of Radix Isatidis, 9 parts of calamus, 8 parts of Golden Larch Bark, 6 parts of Herba Andrographitis, 7 parts of the tuber of stemona.
The method of the present invention for preparing the medicine for preventing and treating grapevine anthracnose, comprises the following steps：
（1）Each composition is weighed according to proportioning, particle diameter is crushed to pulverizer respectively for 100 mesh, and is well mixed, mixed powder is obtained
Material；
（2）Mixed powder is placed in apparatus,Soxhlet's, under the conditions of 78 DEG C, in the in the mixed solvent refluxing extraction of ethanol and water
5h, separation, respectively obtains solid extract and extract solution；
（3）Then the solid extract after separation is placed in flask, the refluxing extraction that adds water 2h, filters, obtain filtrate and filter residue,
Filter residue, merging filtrate and washing lotion are washed, obtains extracting mixed liquor；
（4）By step（2）Obtained extract solution and step（3）Obtained extraction mixed liquor merges, and concentration, spray-on process is prepared
Drug powder.
Step（2）The ethanol of middle addition and the mixed solution quality of water are 18 times of mixed powder quality, ethanol and water
Volume ratio is 1:1.5.
Step（3）The amount of middle addition water is 18 times of mixed powder quality.
Step（3）It is middle washing filter residue method be：Filter residue is placed in beaker, the water of filter residue 5 times of quality of quality is added, in
Ultrasonic agitation 10min in ultrasonic wave, filtering, you can.
Step（4）Middle use Rotary Evaporators are concentrated to mixed liquor, and mixed liquor is concentrated into former mixed powder matter
5 times of amount.
The medicinal application for preventing and treating grapevine anthracnose that the present embodiment is prepared is during grape growth, without obvious insect pest
Influence, disease rate reduces by 92% than the grape without using medicine, and vintage improves 23%.
Embodiment 2：
The present embodiment is related to a kind of medicine and preparation method for preventing and treating grapevine anthracnose；
The medicine is prepared from by following component：
18 parts of Artemisia spharocephala, 15 parts of car mulberry son, 16 parts of cordate houttuynia, 15 parts of delavay pararuellia herb, 10 parts of fiveleaf carpetweet herb, 18 parts of green bristlegrass, neem bark
15 parts, 15 parts of Radix Isatidis, 12 parts of calamus, 7 parts of Golden Larch Bark, 9 parts of Herba Andrographitis, 6 parts of the tuber of stemona.
The method of the present invention for preparing the medicine for preventing and treating grapevine anthracnose, comprises the following steps：
（1）Each composition is weighed according to proportioning, particle diameter is crushed to pulverizer respectively for 100 mesh, and is well mixed, mixed powder is obtained
Material；
（2）Mixed powder is placed in apparatus,Soxhlet's, under the conditions of 78 DEG C, in the in the mixed solvent refluxing extraction of ethanol and water
5h, separation, respectively obtains solid extract and extract solution；
（3）Then the solid extract after separation is placed in flask, the refluxing extraction that adds water 2h, filters, obtain filtrate and filter residue,
Filter residue, merging filtrate and washing lotion are washed, obtains extracting mixed liquor；
（4）By step（2）Obtained extract solution and step（3）Obtained extraction mixed liquor merges, and concentration, spray-on process is prepared
Drug powder.
Step（2）The ethanol of middle addition and the mixed solution quality of water are 16 times of mixed powder quality, ethanol and water
Volume ratio is 1:1.5.
Step（3）The amount of middle addition water is 16 times of mixed powder quality.
Step（3）It is middle washing filter residue method be：Filter residue is placed in beaker, the water of filter residue 5 times of quality of quality is added, in
Ultrasonic agitation 10min in ultrasonic wave, filtering, you can.
Step（4）Middle use Rotary Evaporators are concentrated to mixed liquor, and mixed liquor is concentrated into former mixed powder matter
5 times of amount.
The medicinal application for preventing and treating grapevine anthracnose that the present embodiment is prepared is during grape growth, without obvious insect pest
Influence, disease rate reduces by 90% than the grape without using medicine, and vintage improves 22%.
Embodiment 3：
The present embodiment is related to a kind of medicine and preparation method for preventing and treating grapevine anthracnose；
The medicine is prepared from by following component：
10 parts of Artemisia spharocephala, 8 parts of car mulberry son, 15 parts of cordate houttuynia, 13 parts of delavay pararuellia herb, 6 parts of fiveleaf carpetweet herb, 16 parts of green bristlegrass, neem bark 10
Part, 12 parts of Radix Isatidis, 7 parts of calamus, 6 parts of Golden Larch Bark, 5 parts of Herba Andrographitis, 5 parts of the tuber of stemona.
The method of the present invention for preparing the medicine for preventing and treating grapevine anthracnose, comprises the following steps：
（1）Each composition is weighed according to proportioning, particle diameter is crushed to pulverizer respectively for 80 mesh, and is well mixed, mixed powder is obtained；
（2）Mixed powder is placed in apparatus,Soxhlet's, under the conditions of 75 DEG C, in the in the mixed solvent refluxing extraction of ethanol and water
6h, separation, respectively obtains solid extract and extract solution；
（3）Then the solid extract after separation is placed in flask, the refluxing extraction that adds water 1h, filters, obtain filtrate and filter residue,
Filter residue, merging filtrate and washing lotion are washed, obtains extracting mixed liquor；
（4）By step（2）Obtained extract solution and step（3）Obtained extraction mixed liquor merges, and concentration, spray-on process is prepared
Drug powder.
Step（2）The ethanol of middle addition and the mixed solution quality of water are 10 times of mixed powder quality, ethanol and water
Volume ratio is 1:0.5.
Step（3）The amount of middle addition water is 10 times of mixed powder quality.
Step（3）It is middle washing filter residue method be：Filter residue is placed in beaker, the water of filter residue 3 ~ 8 times of quality of quality is added,
The ultrasonic agitation 5min in ultrasonic wave, filtering, you can.
Step（4）Middle use Rotary Evaporators are concentrated to mixed liquor, and mixed liquor is concentrated into former mixed powder matter
3 times of amount.
The medicinal application for preventing and treating grapevine anthracnose that the present embodiment is prepared is during grape growth, without obvious insect pest
Influence, disease rate reduces by 88% than the grape without using medicine, and vintage improves 19%.
Embodiment 4：
The present embodiment is related to a kind of medicine and preparation method for preventing and treating grapevine anthracnose；
The medicine is prepared from by following component：
20 parts of Artemisia spharocephala, 17 parts of car mulberry son, 22 parts of cordate houttuynia, 21 parts of delavay pararuellia herb, 11 parts of fiveleaf carpetweet herb, 24 parts of green bristlegrass, neem bark
18 parts, 20 parts of Radix Isatidis, 15 parts of calamus, 10 parts of Golden Larch Bark, 11 parts of Herba Andrographitis, 8 parts of the tuber of stemona.
The method of the present invention for preparing the medicine for preventing and treating grapevine anthracnose, comprises the following steps：
（1）Each composition is weighed according to proportioning, particle diameter is crushed to pulverizer respectively for 150 mesh, and is well mixed, mixed powder is obtained
Material；
（2）Mixed powder is placed in apparatus,Soxhlet's, under the conditions of 80 DEG C, in the in the mixed solvent refluxing extraction of ethanol and water
4h, separation, respectively obtains solid extract and extract solution；
（3）Then the solid extract after separation is placed in flask, the refluxing extraction that adds water 2h, filters, obtain filtrate and filter residue,
Filter residue, merging filtrate and washing lotion are washed, obtains extracting mixed liquor；
（4）By step（2）Obtained extract solution and step（3）Obtained extraction mixed liquor merges, and concentration, spray-on process is prepared
Drug powder.
Step（2）The ethanol of middle addition and the mixed solution quality of water are 20 times of mixed powder quality, ethanol and water
Volume ratio is 1: 2.
Step（3）The amount of middle addition water is 20 times of mixed powder quality.
Step（3）It is middle washing filter residue method be：Filter residue is placed in beaker, the water of filter residue 8 times of quality of quality is added, in
Ultrasonic agitation 10min in ultrasonic wave, filtering, you can.
Step（4）Middle use Rotary Evaporators are concentrated to mixed liquor, and mixed liquor is concentrated into former mixed powder matter
5 times of amount.
The medicinal application for preventing and treating grapevine anthracnose that the present embodiment is prepared is during grape growth, without obvious insect pest
Influence, disease rate reduces by 89% than the grape without using medicine, and vintage improves 20%.
In summary, the medicine of the present invention for preventing and treating grapevine anthracnose, the medicinal application during wine-growing,
The infringement of anthrachose of grape can not only be effectively prevented and treated, effect improves grape quality significantly, also long with efficacy time, safety non-pollution
The characteristics of, it is to avoid because grape eats unsafe phenomenon caused by medicament residue, with preferable application value.In addition,
The preparation method of the described medicine for preventing and treating grapevine anthracnose is simple and convenient to operate, and process conditions are gentle, is adapted to industrialization promotion
Production.
The specific embodiment of the present invention is described above.It is to be appreciated that the invention is not limited in above-mentioned
Particular implementation, those skilled in the art can make various deformations or amendments within the scope of the claims, this not shadow
Ring the substantive content of the present invention.
Claims (9)
1. a kind of medicine for preventing and treating grapevine anthracnose, it is characterised in that include each component of following parts by weight：Artemisia spharocephala 10 ~
20 parts, 8 ~ 17 parts of car mulberry son, 15 ~ 22 parts of cordate houttuynia, 13 ~ 21 parts of delavay pararuellia herb, 6 ~ 11 parts of fiveleaf carpetweet herb, 16 ~ 24 parts of green bristlegrass, chinaberry
10 ~ 18 parts of skin, 12 ~ 20 parts of Radix Isatidis, 7 ~ 15 parts of calamus, 6 ~ 10 parts of Golden Larch Bark, 5 ~ 11 parts of Herba Andrographitis, 5 ~ 8 parts of the tuber of stemona.
2. the medicine according to claim 1 for preventing and treating grapevine anthracnose, it is characterised in that 13 parts of the Artemisia spharocephala, Che Sang
Young 12 parts, 19 parts of cordate houttuynia, 16 parts of delavay pararuellia herb, 8 parts of fiveleaf carpetweet herb, 20 parts of green bristlegrass, 13 parts of neem bark, 18 parts of Radix Isatidis, calamus 9
Part, 8 parts of Golden Larch Bark, 6 parts of Herba Andrographitis, 7 parts of the tuber of stemona.
3. the medicine according to claim 1 for preventing and treating grapevine anthracnose, it is characterised in that 18 parts of the Artemisia spharocephala, Che Sang
Young 15 parts, 16 parts of cordate houttuynia, 15 parts of delavay pararuellia herb, 10 parts of fiveleaf carpetweet herb, 18 parts of green bristlegrass, 15 parts of neem bark, 15 parts of Radix Isatidis, calamus
12 parts, 7 parts of Golden Larch Bark, 9 parts of Herba Andrographitis, 6 parts of the tuber of stemona.
4. the method that one kind prepares the medicine for preventing and treating grapevine anthracnose as described in any one of claim 1 ~ 3, it is characterised in that
Comprise the following steps：
（1）Each composition is weighed according to proportioning, is crushed respectively with pulverizer, and is well mixed, mixed powder is obtained；
（2）Mixed powder is placed in apparatus,Soxhlet's, under the conditions of 75 ~ 80 DEG C, flowed back in the in the mixed solvent of ethanol and water
4 ~ 6h is extracted, separation respectively obtains solid extract and extract solution；
（3）Then the solid extract after separation is placed in flask, 1 ~ 2h of the refluxing extraction that adds water, filters, obtain filtrate and filter
Slag, washs filter residue, merging filtrate and washing lotion, obtains extracting mixed liquor；
（4）By step（2）Obtained extract solution and step（3）Obtained extraction mixed liquor merges, and concentration, spray-on process is prepared
Drug powder.
5. the preparation method of the medicine according to claim 4 for preventing and treating grapevine anthracnose, it is characterised in that the step
（1）Particle diameter after middle crushing is 80 ~ 150 mesh.
6. the preparation method of the medicine according to claim 4 for preventing and treating grapevine anthracnose, it is characterised in that the step
（2）The ethanol of middle addition and the mixed solution quality of water are 10 ~ 20 times of mixed powder quality, and the volume ratio of ethanol and water is 1:
0.5~2。
7. the preparation method of the medicine according to claim 4 for preventing and treating grapevine anthracnose, it is characterised in that the step
（3）The amount of middle addition water is 10 ~ 20 times of mixed powder quality.
8. the preparation method of the medicine according to claim 4 for preventing and treating grapevine anthracnose, it is characterised in that the step
（3）It is middle washing filter residue method be：Filter residue is placed in beaker, the water of filter residue 3 ~ 8 times of quality of quality is added, surpassed in ultrasonic wave
Sound stirs 5 ~ 10min, filtering, you can.
9. the preparation method of the medicine according to claim 4 for preventing and treating grapevine anthracnose, it is characterised in that the step
（4）Middle use Rotary Evaporators are concentrated to mixed liquor, and mixed liquor is concentrated into 3 ~ 5 times of former mixed powder quality.
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| CN115181977B (en) * | 2022-07-15 | 2023-09-15 | 重庆工业职业技术学院 | Preparation method of houttuynia cordata extract, houttuynia cordata extract and application of houttuynia cordata extract |
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Application publication date: 20171010 |