CN107232233A - It is a kind of to be used to prevent and treat the bactericidal composition of powdery mildew and its applied in powdery mildew is prevented and treated - Google Patents
It is a kind of to be used to prevent and treat the bactericidal composition of powdery mildew and its applied in powdery mildew is prevented and treated Download PDFInfo
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- CN107232233A CN107232233A CN201710598813.9A CN201710598813A CN107232233A CN 107232233 A CN107232233 A CN 107232233A CN 201710598813 A CN201710598813 A CN 201710598813A CN 107232233 A CN107232233 A CN 107232233A
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- bactericidal composition
- cfu
- powdery mildew
- physcion
- liben
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
Abstract
The invention provides a kind of bactericidal composition for being used to prevent and treat powdery mildew, including Physcion and Ke Liben series bacillus liquid；The quality of Physcion and the volume ratio of Ke Liben series bacillus liquid are (0.2~0.8) mg：100mL；The content of Ke Liben series bacillus is 0.5 × 10 in the bactericidal composition7CFU/mL~1.2 × 107CFU/mL.The bactericidal composition that the present invention is provided, wherein Ke Liben series bacillus Physcion complex biological bactericide can replace the use of chemical pesticide, reduce residues of pesticides, introduce beneficial microbe, improve the ecological environment of vegetable crop, and then improve vegetable yields and quality.Meanwhile, the bactericidal composition that provides of the present invention is used for the Toxicity Determination for suppressing powdery mildew of cucumber bacteria growing, illustrate that Physcion and Ke Liben series bacillus are used cooperatively with obvious syneryistic effect, with greatly improving sterilization drug effect.
Description
Technical field
The present invention relates to biological pesticide bactericide technical field, and in particular to a kind of bactericidal composition and its preventing and treating white powder
Applied in disease.
Background technology
In domestic Agricultural Activities, due to fungi, bacterium, crop leaf disease is tight all the more caused by nematode and virus etc.
Weight, in addition anemophily is propagated, and addition crop leaf is easily occurred the generation that cross-infection has aggravated disease.Common powdery mildew
Have：Wheat powdery mildew, powdery mildew of strawberry, uncinula necator, powdery mildew of cucumber, peach powdery mildew.Specific powdery mildew in seedling and
Reproduction reproductive phase all easily occurs, and powdery mildew disease can not only easily cause death of seedling, lacks the disconnected ridge of row, can also influence crop
Yield and quality.
At present, the preventing and treating of powdery mildew mainly has following several method：One is that strain of catching an illness strictly is rejected when buying nursery stock,
Prevent pathogeny.Two be when carrying out expanding numerous, that clip is transplanted without disease pest or root turion is as asexual propagation material.Three be crop field pipe
In reason period, dispenser preventing and treating is carried out, prevent the incoming new district of crop in spite of illness.Four are mainly adopted for the effect for having infected powdery mildew
Sprayed with medicine, growth of such as kresoxim-methyl to spore germination and leaf intramatrical mycelium has very strong inhibitory action, with protecting, control
Treat and root out activity；Ethirimol has extremely strong killing effect to mycelium, conidium, receptive hypha etc., and can strongly suppress
The formation of spore, blocks spore to infect source again, bactericidal effect is comprehensively thorough；Flusilazole Prochloraz is absorbability triazole pesticide
Bactericide, is sprayed after plant leaf surface in medicine, can be conducted in plant rapidly by Foliage Absorption, suppress ergosterol
Biosynthesis, thus hinder mycelia growth, development, so as to reach the effect prevented and cured diseases.Although above-mentioned chemical pesticide energy
Enough generations for effectively preventing and treating powdery mildew spread, but there is the high defect of persticide residue, not only influence the product of vegetable crop
Matter can also cause environmental pollution.
The content of the invention
In view of this, it is an object of the invention to a kind of bactericidal composition for being used to prevent and treat powdery mildew, by using rheum officinale
Plain methyl ether and Ke Liben series bacillus is compounded, obtain a kind of synergy substantially and nonhazardous bactericidal composition.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：
The invention provides a kind of bactericidal composition for being used to prevent and treat powdery mildew, including Physcion and Ke Liben class buds
Spore bacillus；The quality of Physcion and the volume ratio of Ke Liben series bacillus are (0.2~0.8) mg：100ml；It is described to kill
The content of Ke Liben series bacillus is 0.5 × 10 in bacteria composition7CFU/mL~1.2 × 107CFU/mL。
It is preferred that, the content of Ke Liben series bacillus is 0.7 × 10 in the bactericidal composition7CFU/mL~1.0 ×
107CFU/mL。
It is preferred that, the quality of Physcion and the volume ratio of Ke Liben series bacillus are (0.3~0.6) mg：
100mL。
It is preferred that, the quality of Physcion and the volume ratio of Ke Liben series bacillus are 0.5mg：100mL.
It is preferred that, the formulation of the bactericidal composition is aqua；The aqua includes following content component：Mass percent
For 0.3~1.0% Physcion, mass percent is 0.8~1.5% dispersant, and Ke Liben series bacillus is in water
Cell concentration is 0.7~1.0 × 10 in agent7CFU/mL。
It is preferred that, the aqua includes following content component：Mass percent is 0.5% Physcion, quality hundred
Divide than the dispersant for 1%, Ke Liben series bacillus cell concentration in aqua is 0.8 × 107CFU/mL。
It is preferred that, the dispersant is 2- naphthalene sulfonic acid formaldehyde polymer sodium salts.
The invention provides application of the bactericidal composition in powdery mildew is prevented and treated.
It is preferred that, the powdery mildew is powdery mildew of cucumber and uncinula necator.
It is preferred that, the amount of application of bactericidal composition is 120~200ml/ mus.
The invention provides a kind of bactericidal composition for being used to prevent and treat powdery mildew, including Physcion and Ke Liben class buds
Spore bacillus；The quality of Physcion and the volume ratio of Ke Liben series bacillus are (0.2~0.8) mg：100mL；It is described to kill
The content of Ke Liben series bacillus is 0.5 × 10 in bacteria composition7CFU/mL~1.2 × 107CFU/mL.What the present invention was provided
Bactericidal composition, wherein Ke Liben series bacillus Physcion complex biological bactericide can replace making for chemical pesticide
With Physcion belongs to the medicine of plant source extraction, and Ke Liben series bacillus bacterium solution, which less exists, introduces harmful poisonous thing
Matter is remained, and improves the micro-ecological environment of crop soil, makes the growing environment of crop more preferable, so that residues of pesticides are reduced,
Beneficial microbe is introduced, improves the ecological environment of vegetable crop, and then improve vegetable yields and quality.
Meanwhile, the bactericidal composition that the present invention is provided is used for the Toxicity Determination for suppressing powdery mildew of cucumber bacteria growing, says
Bright Physcion and Ke Liben series bacillus use cooperatively have obvious syneryistic effect, the bactericidal composition with
Physcion is used alone to compare with bactericide prepared by Ke Liben series bacillus with sterilization drug effect is greatly improved, reduces
Usage amount and the negative effect to environment.
Embodiment
The invention provides a kind of bactericidal composition for being used to prevent and treat powdery mildew, including Physcion and Ke Liben class buds
Spore bacillus；The quality of Physcion and the volume ratio of Ke Liben series bacillus are (0.2~0.8) mg：100mL；It is described to kill
The content of Ke Liben series bacillus is 0.5 × 10 in bacteria composition7CFU/mL~1.2 × 107CFU/mL。
In the present invention, the quality of Physcion and the volume ratio of Ke Liben series bacillus are preferably (0.3~0.6)
mg：100mL, most preferably 0.5mg：100mL.In the present invention, the source of the Physcion is not particularly limited using this
Physcion known to art personnel.The source of the Ke Liben series bacillus is not particularly limited use
The source of Ke Liben series bacillus well-known to those skilled in the art.
In the present invention, the content of Ke Liben series bacillus is preferably 0.7 × 10 in the bactericidal composition7CFU/mL~
1.0×107CFU/mL, more preferably 0.89 × 107CFU/mL.The Ke Liben series bacillus preferably refers to living spores quantity.
In the present invention, the formulation of the bactericidal composition is preferably aqua；The aqua preferably includes following content component：
Mass percent is 0.3~1.0% Physcion, and mass percent is 0.8~1.5% dispersant, Ke Liben class buds
Spore bacillus cell concentration in aqua is 0.7~1.0 × 107CFU/mL；More preferably：Mass percent is 0.5% rheum officinale
Plain methyl ether, mass percent is 1% dispersant, and Ke Liben series bacillus cell concentration in aqua is 0.8 × 107CFU/
mL。
In the present invention, the dispersant is preferably 2- naphthalene sulfonic acid formaldehyde polymer sodium salts.The 2- naphthalenesulfonateformaldehyde formaldehydes polymerization
The source of thing sodium salt is not particularly limited using 2- naphthalene sulfonic acid formaldehyde polymers sodium salt well-known to those skilled in the art.
In the present invention, the preparation method of the bactericidal composition aqua preferably includes following steps：
Ke Liben series bacillus, Physcion and dispersant stirring, obtain bactericidal composition aqua.
In the present invention, the Ke Liben series bacillus is preferably diluted to 0.7~1.0 × 10 before combination7CFU/mL.Institute
The method for stating dilution is not particularly limited, using dilution process well-known to those skilled in the art.The thinned water
Preferably distilled water or sterilized water.
In the present invention, the method for the mixing is not particularly limited using mixed method well-known to those skilled in the art
.The method of the stirring is preferably to be not particularly limited, using stirring means well-known to those skilled in the art.
The speed of the stirring is preferably 100~300rpm, more preferably 200rpm.
The invention provides application of the bactericidal composition in powdery mildew is prevented and treated.
In the present invention, the powdery mildew is preferably powdery mildew of cucumber and uncinula necator.
In the present invention, the amount of application of bactericidal composition is preferably 120~200ml/ mus, more preferably 160ml/ mus.It is described
The mode that the application process of bactericidal composition is preferred to use sprinkling is carried out.Preferably in the morning 8 points~9 points of the time of the sprinkling or
4~5 points of progress in person's afternoon.
A kind of bactericidal composition and its application provided with reference to embodiment the present invention is described in detail, still
They can not be interpreted as limiting the scope of the present invention.
Embodiment 1
Physcion Ke Liben series bacillus aqua suppresses the measure of powdery mildew of cucumber bacteria growing
1st, medicament configuration
The decoction of various concentrations is prepared with water on aseptic operating platform：With the Physcion of weight/mass percentage composition 4.5%
It is diluted and is configured to 0.5% solution respectively；It is 0.1 × 10 with spore concentration8Cfu/ml Ke Liben series bacillus difference
Dilution is configured to 1.0 × 107cfu/ml。
It is 0.1 × 10 by 4.5% Physcion and spore concentration of above-mentioned weight/mass percentage composition8Cfu/ml gram in
This series bacillus is compounded, and is formulated as follows the bactericidal composition of concentration：0.2%+0.5 × 107Cfu/ml, 0.8%+1.2
×107Cfu/ml, 0.5%+0.8 × 107Cfu/ml, 0.2%+1.2 × 107Cfu/ml, 0.8%+0.3 × 107Cfu/ml and
0.1%+0.5 × 107cfu/ml。
In the PDA culture medium for taking 1ml to add 50 DEG C respectively the medicament of 8 kinds of various concentrations of above-mentioned configuration, plate is down flat, is obtained
To the PDA plate of drug-treated.To add the PDA plate of isometric water as control.
Put down for examination cucumber powdery mildew's pathogen (Rhizoctonia solani K ü hn) bacterial strain in the PDA that drug-treated is not used
Activated on plate culture medium, the stand-by pathogen bacteria cake cultivated in PDA culture medium is intercepted with card punch, mycelia is inoculated in up
Pastille PDA culture medium (or control medium) center solidified, is placed in dark culturing at 25 DEG C, colony diameter length to be compareed is extremely
During more than plate diameter 2/3, with kind of calliper colony diameter (mm).Each bacterium colony crossing method vertical survey diameter each 1
It is secondary, take its average value.Often processing is repeated 4 times, and the growth inhibition ratio that each chemicals treatment extends to bacterium colony is calculated with comparing.With
The logarithm value of pharmacy quality concentration is that abscissa, the niqueMin of mycelial growth inhibition rate are ordinate, using regression analysis pair
Test data is analyzed, and calculates EC50 values, EC90 values and its 95% confidence limit of each processing.
Experimental data is as shown in table 1.
The compound disinfectant of table 1 and control group bacteriostasis rate list
Embodiment 2
This series bacillus aqua prevents and treats the field of powdery mildew of cucumber in 0.5% 0.1 hundred million living spores of Physcion/ml grams
Between plot experiment：
1st, medicament configuration
The decoction of various concentrations is prepared with water on aseptic operating platform：With the Physcion of weight/mass percentage composition 4.5%
It is diluted and is configured to 0.5% solution respectively；It is 0.1 × 10 with spore concentration8Cfu/ml Ke Liben series bacillus difference
Dilution is configured to 1.0 × 107cfu/ml。
It is 0.1 × 10 by 4.5% Physcion and spore concentration of above-mentioned weight/mass percentage composition8Cfu/ml gram in
This series bacillus is compounded, and is formulated as follows the bactericidal composition of concentration：0.2%+0.5 × 107Cfu/ml, 0.8%+1.2
×107Cfu/ml, 0.5%+0.8 × 107Cfu/ml, 0.2%+1.2 × 107Cfu/ml, 0.8%+0.3 × 107Cfu/ml and
0.1%+0.5 × 107cfu/ml。
By the Physcion solution of above-mentioned 6 kinds of combination sterilization compositions and various concentrations, Ke Liben series bacillus bacterium
Suspension carries out the test of pesticide effectiveness, and and comparison medicine respectively to the biological influence of cucumber garden natural enemy and the other influences to crop production
Agent is compared, and technical data is provided for agriculture chemical registration.
2nd, experimental condition
The selection of 2.1 subjects, crop and kind
Subjects：Powdery mildew of cucumber (Erysiphe cichoracearum, Sphaerotheca fuliginea).
Trial crops：Cucumber, kind is " excellent No. 4 of Tianjin ".
2.2 environmental condition
Experiment selection is carried out in the big riverside village Vegetable Base in Ji'an City Jizhou District Zhang Shan towns, experimental field powdery mildew of cucumber over the years
Occurring and damage is heavier, and experiment purpleflower violet herb is summer sowing, is that the greenhouse of in early June, 2014 is live, and every mu is planted 3000 plants or so, experiment
When cucumber grow fine, in seedling stage, powdery mildew of cucumber is has not occurred.Soil, cultivation and the water and fertilizer management of each experimental plot
Etc. consistent, and meet the agricultural production practice of local science.
3rd, experimental design and arrangement
3.1 medicament
Comparison medicament
50% azoxystrobin water dispersible granule, Jiangxi Tianren Ecology Co., Ltd. provides.
Dosing is shown in Table 2. with processing numbering
The reagent agent experimental design of table 2
3.2 cell arrangements
Plot area：20m2。
Number of repetition：4 repetitions.
3.3 application method
3.3.1 application method
Using spray-on process dispenser, complete stool leave dual sides carry out even spraying.
3.3.2 spraying equipment
3WBD-16 types (laterite board) knapsack electric sprayer carries out even spraying, and (sprayer is tangential centrifugal whole
Body single spraying head, injection diameter 1.3mm, 2~4kg/cm of operating pressure2)。
3.3.3 spraying time and number of times
In on June 21st, 2014 powdery mildew of cucumber do not fall ill also and apply first time medicine, June 28 (downy mildew takes place), 7
The moon respectively applies a medicine on 5th again, and three medicines are applied altogether.Cucumber grows fine, in seedling stage.
3.3.4 capacity is used
According to experimental design dosage vehicle, every mu is watered 50 liters with decoction.
3.3.5 the medicament data of other pest and disease damages is prevented and treated
The other pest and disease damages of pesticide control and weeds were not applied during experiment and before dispenser.
4 investigation, record and measuring method
4.1 meteorological and soil informations
4.1.1 meteorological data
(June 21) is cloudy day, 1-2 grades of south wind, 23~28 DEG C of temperature on the day of first time dispenser；After medicine 1~7 day with cloudy
Or based on rainy weather, 23~34 DEG C of temperature.(June 28) is the cloudy day on the day of second of dispenser, 1~2 grade of south wind, temperature 25~
33℃；After medicine 1~7 day based on cloudy or light rain weather, 25~34 DEG C of temperature.(July 5) is many on the day of third time dispenser
Cloud, 1~2 grade of south wind, 26~33 DEG C of temperature；After medicine 1~7 day based on cloudy or rainy weather, 25~37 DEG C of temperature.Dispenser is worked as
Day experimental field weather conditions and experiment during meteorological data summary be shown in Table 4,5.
4.1.2 soil information
Experimental field soil is sandy loam, and pH value 6.8 or so, soil property is preferable, and the content of organic matter is general, and fertility level is medium,
Irrigation and drainage are convenient, and 2600 kilograms or so of night soil is applied for every mu before field planting, and 50 kilograms of N, P, K composite fertilizer makees base fertilizer, field artificial weeding,
Weeds are less.
4.2 investigation methods, time and number of times
4.2.1 control time and number of times
7 days after first time medicine (before second of medicine), 7 days after second of medicine (before third time medicine) and third time (last time)
Respectively investigate a drug effect within 10 days after medicine, investigate 3 times altogether.
4.2.2 investigation method
Random 4 points of samplings per cell, every is investigated 2 plants, investigates 8 plants of cucumber, the whole blades of every plant of investigation, system altogether per cell
Total number of sheets, the sick number of sheets and corresponding diseased plant number are counted, disease index and prevention effect is calculated.
Severity Scaling is carried out by following standard.
0 grade：Disease-free spot；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 6%~10% of whole leaf area；
5 grades：Lesion area accounts for the 11~20% of whole leaf area；
7 grades：Lesion area accounts for whole leaf area 21%~40%；
9 grades：Lesion area accounts for more than the 40% of whole leaf area.
4.2.3 drug effect computational methods
A, the survey data and result of calculation for details see attached table 3 of respectively four repetitions of processing.
B, drug effect are calculated and carried out as follows：
In formula：Disease index after disease index after PT1-chemicals treatment district medicine, Ck1-blank district medicine.
4.3 directly affect to crop
Each chemicals treatment district cucumber grows normally during experiment, and poisoning and other harmful effects do not occur.
The yield and quality of 4.4 products
According to test protocols, it is not necessary to carry out the record of yield and quality.
4.5 pairs of other biotic influences
4.5.1 to the influence of other pest and disease damages
Observed during experiment, influence of the test medicine to other Pest organisms etc. be not obvious.
4.5.2 to the influence of other non-target organisms
Observed during experiment, influence of the test medicine to natural enemy around and beneficial organism be not obvious.
Biometric analysis method:With Deng Kenshi duncan's new multiple range methods (DMRT)
5 results and analysis
Compound biocide composite, 0.1 hundred million this series bacillus (the original liquid of fermentation), 0.5% rheum emodin first in cfu/ml grams
Ether aqua and 50% azoxystrobin water dispersible granule prevent and treat powdery mildew of cucumber experiment initial data statistical form.
Compound biocide composite and control group are as shown in table 3 to the suppression situation of cucumber powdery mildew's pathogen.
The suppression situation of the compound biocide composite of table 3 and control group to cucumber powdery mildew's pathogen
Evaluating drug:This experiment shows that reagent agent, which handles 1~4 pair of powdery mildew of cucumber, has preferable biocidal property, average
Antibacterial preventive effect reaches 92.6~95.4%, compared with comparison medicament 50% azoxystrobin water dispersible granule, 45 grams/acre of preventive effects, significantly excellent
In 50% azoxystrobin water dispersible granule.0.1 hundred million this series bacillus in cfu/ml grams (the original liquid of fermentation), 0.5% rheum emodin first
Ether aqua has certain suppression and prevention effect to powdery mildew of cucumber, and every mu respectively with 4000 milliliters of preparation, 500 milliliters of continuous sprays
The preventive effect of 10 days is respectively up to 59.90%, 68.34% after Shi Sanci (interval be 7 days), third time medicine, between test medicine
There is significant difference between preventive effect；Milliliter/mu of 0.5% Physcion aqua 500 dissipates with the Fluoxastrobin moisture of comparison medicament 50%
45 grams/acre of preventive effects of granula quite, between be not present significant difference.
Safety analysis：The medicament does not occur poisoning and other abnormal influences to cucumber safety, to natural enemy around and
The influence of beneficial organism is not obvious.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of bactericidal composition for being used to prevent and treat powdery mildew, including Physcion and Ke Liben series bacillus liquid；Rheum officinale
The quality of plain methyl ether and the volume ratio of Ke Liben series bacillus liquid are (0.2~0.8) mg：100ml；In the bactericidal composition
The content of Ke Liben series bacillus is 0.5 × 107CFU/mL~1.2 × 107CFU/mL。
2. bactericidal composition according to claim 1, it is characterised in that Ke Liben classes gemma bar in the bactericidal composition
The content of bacterium is 0.7 × 107CFU/mL~1.0 × 107CFU/mL。
3. bactericidal composition according to claim 1, it is characterised in that the quality and Ke Liben classes of the Physcion
The volume ratio of bacillus liquid is (0.3~0.6) mg：100mL.
4. bactericidal composition according to claim 3, it is characterised in that the quality and Ke Liben classes of the Physcion
The volume ratio of bacillus liquid is 0.5mg：100mL.
5. the bactericidal composition according to Claims 1 to 4 any one, it is characterised in that the agent of the bactericidal composition
Type is aqua；The aqua includes following content component：Mass percent is 0.3~1.0% Physcion, quality percentage
Than the dispersant for 0.8~1.5%, Ke Liben series bacillus cell concentration in aqua is 0.7~1.0 × 107CFU/mL。
6. bactericidal composition according to claim 5, it is characterised in that the aqua includes following content component：Quality
Concentration is 0.5mg/mL Physcion, and mass concentration is 1% dispersant, Ke Liben series bacillus thalline in aqua
Concentration is 0.8 × 107CFU/mL。
7. bactericidal composition according to claim 5, it is characterised in that the dispersant is 2- naphthalene sulfonic acid formaldehyde polymers
Sodium salt.
8. application of the bactericidal composition in powdery mildew is prevented and treated described in claim 1~7 any one.
9. application according to claim 8, it is characterised in that the powdery mildew is powdery mildew of cucumber and uncinula necator.
10. application according to claim 8 or claim 9, it is characterised in that the amount of application of bactericidal composition is 120~200ml/
Mu.
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| CN201710598813.9A CN107232233A (en) | 2017-07-21 | 2017-07-21 | It is a kind of to be used to prevent and treat the bactericidal composition of powdery mildew and its applied in powdery mildew is prevented and treated |
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| CN201710598813.9A CN107232233A (en) | 2017-07-21 | 2017-07-21 | It is a kind of to be used to prevent and treat the bactericidal composition of powdery mildew and its applied in powdery mildew is prevented and treated |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102548418A (en) * | 2009-07-30 | 2012-07-04 | 玛珑生物创新有限公司 | Plant pathogen inhibitor combinations and methods of use |
| CN103756931A (en) * | 2013-12-05 | 2014-04-30 | 江西天人生态股份有限公司 | Paenibacillus kribbensis and its application |
-
2017
- 2017-07-21 CN CN201710598813.9A patent/CN107232233A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102548418A (en) * | 2009-07-30 | 2012-07-04 | 玛珑生物创新有限公司 | Plant pathogen inhibitor combinations and methods of use |
| CN103756931A (en) * | 2013-12-05 | 2014-04-30 | 江西天人生态股份有限公司 | Paenibacillus kribbensis and its application |
Non-Patent Citations (1)
| Title |
|---|
| 张光: "农业科技通讯", 《农业科技通讯》 * |
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