CN107212424A - A kind of extracting method of water-soluble barley diet fiber - Google Patents
A kind of extracting method of water-soluble barley diet fiber Download PDFInfo
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- CN107212424A CN107212424A CN201610284722.3A CN201610284722A CN107212424A CN 107212424 A CN107212424 A CN 107212424A CN 201610284722 A CN201610284722 A CN 201610284722A CN 107212424 A CN107212424 A CN 107212424A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The present invention provides a kind of extracting method of water-soluble barley diet fiber, and the extracting method comprises the following steps:Enzymolysis, again enzymolysis, first inactivation, three enzymolysis, inactivation, ethanol precipitation, redissolution evaporations again, produce water-soluble barley diet fiber solution first.The barley diet fiber recovery rate of the present invention is high, can lift the utilization rate of raw material, reduces the generation of waste material, and the research and development and production for barley diet fiber beverage and other Related products provide technical support and reduce cost of material.The present invention compares traditional extraction process recovery rate and is obviously improved.
Description
Technical field
The invention belongs to field of food, and in particular to a kind of extracting method of water-soluble barley diet fiber.
Background technology
Dietary fiber is a kind of polysaccharide, and it can neither be absorbed by pipe intestinal digesting, can not produce energy.Therefore, Zeng Yi
Degree is considered as a kind of apotrophic material and cannot get enough attention for a long time.However, with nutrition and related science
Deeply development, people are gradually found that dietary fiber has considerable physiological action.So that dietary fiber turns into science
Boundary and the material of ordinary people concern, and the 7th class nutrient, and six traditional class nutrition are regarded as by nutrition educational circles supplement
Element --- protein, fat, carbohydrate, vitamin, mineral matter and water are arranged side by side.
Highland barley has abundant nutritive value and prominent medicines and health protection effect.In the Qinghai-Tibet Platean of severe cold region, why
It is no lack of centenarian, this medical health care function protruded with highland barley effect is undivided.According to《Bencao Shiyi》Record:Highland barley,
During lower gas is wide, strengthen lean power, dehumidifying sweating, antidiarrheal.Tibetan medicine's ancient books and records《Jingzhubencao》More using highland barley as a kind of important drugs, use
In a variety of diseases for the treatment of.
Recent studies shows, the total effectses fiber content 16% of barley diet fiber rich content highland barley, wherein insoluble
Property curative effect fiber 9.68%, soluble curative effect fiber 6.37%, the former is 8 times of wheat, and the latter is 15 times of wheat;Gather one Portugal
Sugared content 6.57%, 0.1 percentage point only lower than oat, is 50 times of wheat.Barley diet fiber is to be dissolvable in water water to inhale again
Water expands, and can be normally present in plant cell liquor and cytoplasm, mainly had by a fiber of microorganism glycolysis in large intestine
Pectin, natural plant gum, viscose etc..This kind of material can stimulate intestines peristalsis, be conducive to excrement to discharge, can Constipation, the carcinoma of the rectum, hemorrhoid
Sore and varix of lower limb;Can the angiocardiopathy such as prevention of arterial atherosis and coronary heart disease generation;Prevent the shape of gall stone
Into.Satiety is produced, adiposis patient is fed favorably, diet food can be used as.Improve resistance to sugar amount, can adjust the blood of diabetes patient
Sugar level, can as diabetes patient food;Improve gut flora, prevention intestinal cancer, appendicitis etc..
In recent years, with the further investigation of dietary fiber, the research and development of related functionality product are received significant attention, and are passed
The barley diet fiber extracting method complex steps of system, recovery rate is relatively low, therefore the present invention has larger actual use value.More
Specifically, the method for dietary fiber is extracted at present mainly has fractionation, enzymatic reagent method, chemical reagent and enzymatic reagent to combine
Method, membrane separation process.But these method recovery rates are relatively low, cost is higher, and extraction step is cumbersome, while to the physics and chemistry of dietary fiber
Property and physiological function have an impact, and are not suitable for industrialization and extract barley diet fiber.At this stage, barley diet fiber is carried
Take the optimizing research of method less, the optimizing research extracted to barley diet fiber hydrogen peroxide method is less, current barley dietary
The extraction of fiber still has many places to need improvement.
The content of the invention
For deficiency of the prior art, it is an object of the invention to provide a kind of extraction side of water-soluble barley diet fiber
Method, specifically extracts water-soluble barley diet fiber using the hydrogen peroxide method of innovation, optimizes barley diet fiber extraction conditions,
The utilization rate of highland barley is improved, the generation of waste material is reduced, and then reduce production cost.Reaction condition of the present invention is gentle, dirty to environment
Dye is smaller, and still further aspect also causes recovery rate to be higher than existing level, meanwhile, the parameters using the present invention can make at present
Barley diet fiber recovery rate brings up to 56.66%, already belongs to breakthrough.The present invention for barley diet fiber beverage and
The research and development and the certain theoretical foundation of production offer and technical support of other related functionality food.
In pilot experiments applicant determine from enlightening celebrate area nine kinds of highland barleys water soluble dietary fiber content and
Water insoluble dietary fiber content, finds the dietary fiber content highest of its Central-South Long Gena highland barley, therefore selection Nan Longgena
Highland barley is used as extraction material.
The purpose of the present invention is achieved through the following technical solutions:
The present invention provides a kind of extracting method of water-soluble barley diet fiber, and the extracting method comprises the following steps:
Digest first:Alpha-amylase is added into the mixed liquor of highland barley flour and water, digests, obtains enzymolysis liquid first;
Digest again:Papain is added into the enzymolysis liquid first, digests, obtains enzymolysis liquid again;
Inactivate first:Inactivation treatment is carried out to the enzymolysis liquid again, the enzymolysis liquid again of inactivation is obtained;
Three enzymolysis:Carbohydrase is added into the enzymolysis liquid again of the inactivation, digests, obtains three enzymolysis liquids;
Inactivate again:It will be inactivated under three enzymolysis liquid water bath conditions of gained, adjust its pH value, plus hydrogen peroxide is extracted and must carried
Take liquid;
Ethanol precipitation:Centrifuge the extract solution and obtain supernatant, add ethanol, stand to obtain precipitation solution;
Redissolve evaporation:Centrifuging the precipitation solution must precipitate, and be deposited under water-bath and redissolved, evaporated with water, produce water-soluble green grass or young crops
Highland barley dietary fiber solution;Wherein, the volume of the water-soluble barley diet fiber solution of gained is after evaporation:Every 80 milliliters of enzyme first
The volume that solution liquid finally obtains water-soluble barley diet fiber solution is 25ml;The water consumption of the redissolution and enzymolysis step first
In water it is identical.
Preferably, in the step of digesting first, the mixed solution is prepared as:Every 10 grams of highland barley flours add water 80 milliliters
(i.e. solid-to-liquid ratio is 1:8);The water is deionized water;Following dosage is using the volume of water as 80 milliliters of calculating.Because most of blue or green
Material in highland barley powder can all sink to container bottom under static conditions in the form of sediment, and these materials will be cast out, real
The dietary fiber aqueous solution obtained during testing is approximately equal to 80ml, therefore is calculated by the volume of water.
Preferably, in the step of digesting first, the alpha-amylase is Thermostable α-Amylase;The alpha-amylase consumption
For:The consumption of every 80 milliliters of water correspondence alpha-amylase is 50 microlitres;The condition of the enzymolysis is:Water-bath 1 hour at 80 DEG C.
Preferably, in the step of digesting again, the Papain enzyme dosage is:Every 80 milliliters of water correspondence papain
Consumption be 50 microlitres;It is described digest condition is:Water-bath 1 hour at 60 DEG C.
Preferably, also need enzymolysis liquid first being cooled to room temperature before digesting again.
Preferably, in the step of inactivating first, the actual conditions of the inactivation is:30min under 100 DEG C of water bath conditions;Go out
Also need to be cooled to preference temperature after work, wherein preference temperature is less than 60 DEG C.
Preferably, in the step of three enzymolysis, the saccharification enzyme dosage is:The consumption of every 80 milliliters of water correspondence carbohydrase is
400 microlitres;The condition of the enzymolysis is:60 DEG C of water-baths 1 hour.
Preferably, in the step of inactivating again, the condition of the inactivation is:100 DEG C of water-bath 30min;The pH value is 6.5
~12;The content of hydrogen peroxide is 0.5~1.5%, and what the percentage was represented is percentage by weight;Oxidation hydrogen content is experiment
Preset value;The time of the extraction is 10~14 hours;Hydrogen peroxide is extracted and carried out at normal temperatures.
It is highly preferred that the pH value includes 6.5,9.2,11.8;The content of hydrogen peroxide include 0.5%, 1.0%,
1.5%;The time of the extraction includes 10 hours, 12 hours, 14 hours.
Preferably, in the step of ethanol precipitation, the condition of the centrifugation includes 3000 revs/min and centrifuged 15 minutes;Add
The volume of ethanol is 3 times of supernatant volume;The standing need to be stayed overnight;Ethanol is added during supernatant is stirred;
The ethanol refers specifically to absolute ethyl alcohol.
Preferably, in the step of redissolving evaporation, the condition of the centrifugation includes 3000 revs/min and centrifuged 15 minutes;Redissolve,
The temperature of evaporation is 80 DEG C.
Preferably, the preparation method is also dry including being freezed to the water-soluble barley diet fiber solution, being freezed
Dry step.
The step of freezing, be freeze-dried be specially:The vessel of water-soluble barley diet fiber solution are will be equipped with sealed membrane
Sealing, is put into refrigerating chamber, above-mentioned redissolution liquid is freezed;After it freezes, the puncture on sealed membrane, and it is put into freeze drier
Middle freeze-drying 24h;Vessel are taken out, the fluffy cake solids of vessel bottom are water-soluble dietary fiber, are scraped, put
In freshness protection package, and preservation in drier is placed on, obtains water-soluble barley diet fiber.
Compared with conventional art, the present invention has following beneficial effect:
(1) by the weighing to barley diet fiber and the contrast with conventional method, the inventive method is obviously improved water-soluble
The recovery rate of property barley diet fiber;
(2) the inventive method is easily realized, production efficiency is high, operating cost it is low (such as the present invention compared with water-swollen squid,
Recovery rate is greatly improved, compared with alkalinity extraction, it is not necessary to dialysis procedure), provide original for the exploitation of related functionality food
Material basis.
Brief description of the drawings
By reading the detailed description made with reference to the following drawings to non-limiting example, further feature of the invention,
Objects and advantages will become more apparent upon:
Fig. 1 compares figure for the recovery rate of six kinds of embodiments;
Fig. 2 is recovery rate highest embodiment or comparative example in water law, alkaline process and hydrogen peroxide method.
Embodiment
With reference to specific embodiment, the present invention is described in detail.Following examples will be helpful to the technology of this area
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that to the ordinary skill of this area
For personnel, without departing from the inventive concept of the premise, various modifications and improvements can be made.These belong to the present invention
Protection domain.
Comparative example 1
This comparative example is related to a kind of water-soluble barley diet fiber extracting method, and methods described comprises the following steps:
Step 1, weigh 10g Nan Longgena highland barley dry weights powder (highland barley polishing after with 5mm aperture sieves sieve), be placed in beaker
In, the deionized water of 40 milliliters of addition stirs equal with glass bar, is placed in water-bath and is incubated to 80 DEG C;
Step 2,50 microlitres of Thermostable α-Amylases of addition, 1h is digested under 80 DEG C of water bath conditions;
Step 3, bath temperature is adjusted to 60 DEG C, weighs papain 15mg and add enzymolysis liquid, in 60 DEG C of water-bath bars
1h is digested under part;
Step 4,30min is inactivated under 100 DEG C of water bath conditions, then temperature is recalled to 60 DEG C;
Step 5,400 microlitres of carbohydrase of addition, 1h is digested under 60 DEG C of water bath conditions;
Step 6,30min is inactivated under 100 DEG C of water bath conditions again, insulation is extracted 3 hours at 60 DEG C again afterwards;
After step 7, extraction terminate, enzymolysis liquid is all poured into centrifugal bottle, 3000 leave the heart 15 minutes, take supernatant
In clean beaker, while being put into stirrer in beaker, in the presence of magnetic stirring apparatus, 3 times of volumes are slowly dropped into anhydrous
Ethanol alcohol precipitation, stands overnight;
Step 8, step 7 gained precipitation solution is put into centrifugal bottle completely, 3000 leave the heart 15 minutes, and supernatant is discarded,
Precipitation is redissolved with original deionized water for adding milliliter number under water bath condition, and is evaporated to 25ml under water bath condition;
Step 9, with sealed membrane beaker is sealed, be put into refrigerating chamber, above-mentioned redissolution liquid is freezed;
Step 10, after it freezes, the puncture on sealed membrane, and be put into freeze drier and be freeze-dried 24h;
Step 11, beaker taken out, the fluffy cake solids of beaker bottom are dietary fiber, are scraped, and are placed in guarantor
In fresh bag, and preservation in drier is placed on, while calculating barley diet fiber recovery rate according to weighing result;
Implementation result:The recovery rate of this comparative example water solubility barley diet fiber is 13.34%.
Comparative example 2
This comparative example is the change case of comparative example 1, and change part is only that:
Step 6,30min is inactivated under 100 DEG C of water bath conditions again, insulation is extracted 5 hours at 80 DEG C again afterwards;
Implementation result:The recovery rate of this comparative example water solubility barley diet fiber is 17.21%.
Comparative example 3
This comparative example is related to a kind of water-soluble barley diet fiber extracting method, and methods described comprises the following steps:
Step 1,10g Nan Longgena highland barley dry weight powder is weighed, be placed in beaker, added 40 milliliters of distilled water, use glass
The stirring of glass rod is equal, is placed in water-bath and is incubated to 80 DEG C;
Step 2,50 microlitres of Thermostable α-Amylases of addition, 1h is digested under 80 DEG C of water bath conditions;
Step 3, bath temperature is adjusted to 60 DEG C, weighs the ㎎ of papain 15 and add enzymolysis liquid, in 60 DEG C of water-bath bars
1h is digested under part;
Step 4,30min is inactivated under 100 DEG C of water bath conditions, then temperature is recalled to 60 DEG C;
Step 5,400 microlitres of carbohydrase of addition, 1h is digested under 60 DEG C of water bath conditions;
Step 6,30min is inactivated under 100 DEG C of water bath conditions again, 3000 leaves the heart 15 minutes afterwards, supernatant is discarded,
Precipitate and washed out with 40 milliliters of 0.5mol/L sodium hydroxide solution, and wash liquid is refunded in former beaker, then at 60 DEG C
3 hours are incubated under bath temperature, then regulation pH is 6.5-6.8 at once;
Step 7, regulation pH after, extract solution is all poured into centrifugal bottle, 3000 leave the heart 15 minutes, take supernatant in
In clean beaker, it is fitted into bag filter, and bag filter is placed in clear water, change within every 12 hours a water, places 24 hours;
Step 8, the liquid in bag filter fallen in clean beaker, while being put into stirrer in beaker, stirred in magnetic force
Mix in the presence of device, be slowly dropped into 3 times of volume absolute ethyl alcohol alcohol precipitations, stand overnight;
Step 9, step 7 gained precipitation solution is put into centrifugal bottle completely, 3000 leave the heart 15 minutes, and supernatant is discarded,
Precipitation is redissolved with original water for adding milliliter number under 80 DEG C of water bath conditions, and is evaporated to 25ml under water bath condition;
Step 10, with sealed membrane beaker is sealed, be put into refrigerating chamber, above-mentioned redissolution liquid is freezed;
Step 11, after it freezes, the puncture on sealed membrane, and be put into freeze drier and be freeze-dried 24h;
Step 12, beaker taken out, the fluffy cake solids of beaker bottom are insoluble dietary fiber, are scraped
Under, it is placed in freshness protection package, and be placed on preservation in drier;
Implementation result:The recovery rate of the water-insoluble barley diet fiber of this comparative example is 21.57%.
Comparative example 4
This comparative example is the change case of comparative example 3, and change part is only that:
Step 6,30min is inactivated under 100 DEG C of water bath conditions again, 3000 leaves the heart 15 minutes afterwards, supernatant is discarded,
Precipitate and washed out with 40 milliliters of 0.5mol/L sodium hydroxide solution, and wash liquid is refunded in former beaker, then at 80 DEG C
5 hours are incubated under bath temperature, then regulation pH is 6.5-6.8 at once;
Implementation result:The recovery rate of the new barley diet fiber of this comparative example non-aqueous is 27.61%.
Embodiment 1
The present embodiment is related to a kind of extracting method of water-soluble barley diet fiber, and methods described comprises the following steps:
Step 1, the highland barley powder for weighing 10g, are placed in beaker, add 80 milliliters of deionized water, are stirred with glass bar
;
Step 2, the Thermostable α-Amylase for adding 50 microlitres, water-bath 1 hour at 80 DEG C;
Step 3, taking-up beaker allow to cool to normal temperature, weigh 15 milligrams of papain and add in enzymolysis liquid,
Water-bath 1 hour at 60 DEG C;
Step 4, under 100 DEG C of water bath conditions 30min is inactivated, be cooled to less than 60 DEG C;
Step 5, add after 400 microlitres of carbohydrase water-bath 1 hour at 60 DEG C;
Step 6, under 100 DEG C of water bath conditions inactivate 30min, with 0.256mol/L sodium hydroxide solution adjust pH value to
6.5, adding the hydrogen peroxide of respective amount makes the concentration of hydrogen peroxide of system be 0.5%, and insulation is extracted 10 hours under normal temperature;
After step 7, extraction terminate, gained enzymolysis is subjected to centrifugal treating, supernatant is taken in clean beaker, simultaneously
Stirrer is put into beaker, in the presence of magnetic stirring apparatus, 3 times of volume absolute ethyl alcohol alcohol precipitations is slowly dropped into, stands overnight;
Step 8, gained precipitation solution is carried out to centrifugal treating, abandoning supernatant, precipitation is existed with original water for adding milliliter number
Redissolved under 80 DEG C of water bath conditions, and 25ml is evaporated under water bath condition;
Step 9, with sealed membrane beaker is sealed, be put into refrigerating chamber, above-mentioned redissolution liquid is freezed;
Step 10, after it freezes, the puncture on sealed membrane, and be put into freeze drier and be freeze-dried 24h;
Step 11 takes out beaker, and the fluffy cake solids of beaker bottom are water-soluble dietary fiber, are scraped, put
In freshness protection package, and preservation in drier is placed on, obtains water-soluble barley diet fiber.
Implementation result:The recovery rate of the present embodiment water solubility barley diet fiber is 36.05%, recovery rate and water law, alkali
Method is compared to rise.
Embodiment 2
The present embodiment is the change case of embodiment 1, and change part is only that:
Step 6, under 100 DEG C of water bath conditions inactivate 30min, with 0.256mol/L sodium hydroxide solution adjust pH value to
9.15, adding the hydrogen peroxide of respective amount makes the concentration of hydrogen peroxide of system be 0.5%, and the lower insulation of normal temperature is extracted 12 hours;
Implementation result:The recovery rate of the present embodiment water solubility barley diet fiber is 44.54%, recovery rate and water law, alkali
Method is compared to rise.
Embodiment 3
The present embodiment is the change case of embodiment 1, and change part is only that:
Step 6, under 100 DEG C of water bath conditions inactivate 30min, with 0.256mol/L sodium hydroxide solution adjust pH value to
6.5, adding the hydrogen peroxide of respective amount makes the concentration of hydrogen peroxide of system be 0.5%, and insulation is extracted 12 hours under normal temperature;
Implementation result:The recovery rate of the present embodiment water solubility barley diet fiber is 38.12%, recovery rate and water law, alkali
Method is compared to rise.
The present invention is during implementation, and step 6 can be achieved under the following conditions:PH value is 6.5~12, hydrogen peroxide
The time that content is 0.5~1.5%, extracted is 10~14 hours.
Embodiment 2 is most highly preferred embodiment of the invention, and water-soluble barley diet fiber recovery rate is 44.54%, is all
Maximum in case.And the highest recovery rate of the water-soluble barley dietary dietary fiber of comparative example is only 27.61%, recovery rate shows
Write rise.The present invention is being obviously improved water-soluble barley diet fiber recovery rate, is the related functional food of soybean starch
Exploitation provides raw material basis.
Comparative example and embodiment are extracted to obtained water-soluble barley diet fiber, recovery rate, knot are calculated using gravimetric method
Fruit is as shown in Figure 1 and Figure 2.
The specific embodiment of the present invention is described above.It is to be appreciated that the invention is not limited in above-mentioned
Particular implementation, those skilled in the art can make various deformations or amendments within the scope of the claims, this not shadow
Ring the substantive content of the present invention.
Claims (10)
1. a kind of extracting method of water-soluble barley diet fiber, it is characterised in that the extracting method comprises the following steps:
Digest first:Alpha-amylase is added into the mixed liquor of highland barley flour and water, digests, obtains enzymolysis liquid first;
Digest again:Papain is added into the enzymolysis liquid first, digests, obtains enzymolysis liquid again;
Inactivate first:Inactivation treatment is carried out to the enzymolysis liquid again, the enzymolysis liquid again of inactivation is obtained;
Three enzymolysis:Carbohydrase is added into the enzymolysis liquid again of the inactivation, digests, obtains three enzymolysis liquids;
Inactivate again:It will be inactivated under three enzymolysis liquid water bath conditions of gained, adjust its pH value, plus hydrogen peroxide is extracted and must extracted
Liquid;
Ethanol precipitation:Centrifuge the extract solution and obtain supernatant, add ethanol, stand to obtain precipitation solution;
Redissolve evaporation:Centrifuging the precipitation solution must precipitate, and be deposited under water-bath and redissolved, evaporated with water, produce water-soluble highland barley meals
Eat fiber solution.
2. the extracting method of water-soluble barley diet fiber according to claim 1, it is characterised in that the step digested first
In rapid, the mixed solution is prepared as:Every 10 grams of highland barley flours add water 80 milliliters.
3. the extracting method of water-soluble barley diet fiber according to claim 1, it is characterised in that the step digested first
In rapid, the alpha-amylase consumption is:The consumption of every 80 milliliters of water correspondence alpha-amylase is 50 microlitres;The condition of the enzymolysis
For:Water-bath 1 hour at 80 DEG C.
4. the extracting method of water-soluble barley diet fiber according to claim 1, it is characterised in that the step digested again
In rapid, the Papain enzyme dosage is:The consumption of every 80 milliliters of water correspondence papain is 50 microlitres;It is described to digest to obtain bar
Part is:Water-bath 1 hour at 60 DEG C.
5. the extracting method of water-soluble barley diet fiber according to claim 1, it is characterised in that the step of three enzymolysis
In rapid, the saccharification enzyme dosage is:The consumption of every 80 milliliters of water correspondence carbohydrase is 400 microlitres;The condition of the enzymolysis is:60
DEG C water-bath 1 hour.
6. the extracting method of water-soluble barley diet fiber according to claim 1, it is characterised in that the step inactivated again
In rapid, the pH value is 6.5~12;The content of hydrogen peroxide is 0.5~1.5%, and what the percentage was represented is weight percent
Than;The time of the extraction is 10~14 hours.
7. the extracting method of water-soluble barley diet fiber according to claim 1, it is characterised in that the step of ethanol precipitation
In rapid, the condition of the centrifugation is centrifuged 15 minutes for 3000 revs/min;The volume for adding ethanol is 3 times of supernatant volume.
8. the extracting method of water-soluble barley diet fiber according to claim 1, it is characterised in that redissolve the step of evaporation
In rapid, the condition of the centrifugation is centrifuged 15 minutes for 3000 revs/min;Redissolve, the temperature of evaporation is 80 DEG C.
9. the extracting method of water-soluble barley diet fiber according to claim 1, it is characterised in that the tool of the inactivation
Concrete conditions in the establishment of a specific crime is:30min under 100 DEG C of water bath conditions.
10. the extracting method of water-soluble barley diet fiber according to claim 1, it is characterised in that the preparation side
The step of method also includes the water-soluble barley diet fiber solution is freezed, is freeze-dried.
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CN109315788A (en) * | 2018-11-22 | 2019-02-12 | 安徽工程大学 | A kind of method of modifying of barley diet fiber |
CN110897082A (en) * | 2019-11-27 | 2020-03-24 | 上海交通大学 | Preparation method of highland barley nutritional rice based on thin-layer coating technology |
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