CN107212422A - A kind of preparation method of algal active peptide - Google Patents
A kind of preparation method of algal active peptide Download PDFInfo
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- CN107212422A CN107212422A CN201710484878.0A CN201710484878A CN107212422A CN 107212422 A CN107212422 A CN 107212422A CN 201710484878 A CN201710484878 A CN 201710484878A CN 107212422 A CN107212422 A CN 107212422A
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- protease
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention discloses a kind of preparation method of algal active peptide, it is characterised in that comprises the following steps：1. protease and food-grade sodium alginate carry out catalyzing hydrolysis, and then the hydrolyzate is inactivated, decolourized and filtration treatment, marine alga polypeptide extract solution is obtained；2. above-mentioned marine alga polypeptide extract solution is purified and concentrated for 1200Da and 500Da NF membrane by molecular weight, obtain concentrate；3. above-mentioned concentrate is passed through into ultra high temperature short time sterilization；4. after sterilizing, algal active peptide powder is obtained by vacuum drying.A kind of preparation method of algal active peptide of the present invention, the preparation method need not add the techniques such as desalination, vacuum concentration, simplify production technology, reduce production cost, the filter residue of nanofiltration separation, filtrate can be used for the production of alga fertilizer as raw material, and resource utilization is high.
Description
Technical field
The present invention relates to a kind of preparation method of active peptide, more particularly to a kind of preparation method of algal active peptide.
Background technology
The particularity of the marine eco-environment, creating marine organisms can produce compared with terrestrial organism secondary metabolite
Unique natural products.In recent years, the research about seaweed bio active peptide is extremely active, although seaweed bio active peptide is ground
Study carefully that history is shorter, but because of its unique biological action, and one of study hotspot as active peptide field.China is to marine alga
Active peptide research and development are started late, and basic and applied research is all very weak, still at an early stage at present, to so far,
Really realize that the marine alga polypeptide products of industrialized production have no to have been reported that, producer still focuses on the common marine alga such as algal polysaccharides and slightly carried
The production of thing.
The content of the invention
It is an object of the present invention to provide a kind of preparation method of algal active peptide, the preparation method need not add desalination,
The techniques such as vacuum concentration, simplify production technology, reduce production cost, the filter residue of nanofiltration separation, filtrate can be used as raw material
In the production of alga fertilizer, resource utilization is high.
Technical scheme：A kind of preparation method of algal active peptide, it is characterised in that comprise the following steps：
1. according to（2~5）︰ 1 protease and food-grade sodium alginate ratio carries out catalyzing hydrolysis, and it is pure that 1~2 times of addition is measured
Water, regulation mixture ph is to 3~4, and temperature is maintained at after 50~75 DEG C, maintains pressure 0.8Mpa hydrolysis 2~3 hours, then
The hydrolyzate is inactivated, decolourized and filtration treatment, marine alga polypeptide extract solution is obtained；
2. above-mentioned marine alga polypeptide extract solution is purified and concentrated for 1200Da and 500Da NF membrane by molecular weight, obtained
Concentrate；
3. above-mentioned concentrate is passed through into ultra high temperature short time sterilization, be put into pressure pan, be forced into 3kgf/cm2, and holding 10~
20min, makes its pressure uniform, then pressure is down to Okgf/cm suddenly at 0~3 second2；
4. after sterilizing, algal active peptide powder is obtained by vacuum drying.
A kind of preparation method of foregoing algal active peptide, the protease includes plant rennet and animal protease,
Protease is with plant rennet and animal protease with 1 ︰（1.2~1.5）Ratio mix.
A kind of preparation method of foregoing algal active peptide, the plant rennet is subtilopeptidase A and pawpaw egg
White enzyme, the animal protease is trypsase and pepsin.
A kind of preparation method of foregoing algal active peptide, the step 1. in, according to 5 ︰ 1 protease and food-grade sea
Mosanom ratio carries out catalyzing hydrolysis, adds the pure water of 1.2 times of amounts, regulation mixture ph is to 4, and temperature is maintained at 75 DEG C
Afterwards, pressure 0.8Mpa is maintained to hydrolyze 3 hours.
Compared with prior art, the present invention is based on plant active peptides enzymolysis process, with marine alga crude extract food-grade sea
Alginic acid sodium raw materials, using the compound coupling hydrolysis process of multienzyme so that marine alga polypeptide mean molecule quantity is solved in 800Da or so
The problem of traditional plant active peptide enzymolysis process molecular weight is uneven.
The present invention isolates and purifies marine alga polypeptide using molecular weight 1200Da and 500Da combination nanofiltration, retains molecule
Amount simplifies production technology in 500~1200Da marine alga polypeptides, without using traditional desalinating process and vacuum concentration process,
Reduce production cost.
Embodiment
The present invention is further illustrated for example below, but is not intended as to the foundation of the invention limited.
Embodiment 1：
A kind of preparation method of algal active peptide, comprises the following steps：
1. catalyzing hydrolysis is carried out according to 5 ︰ 1 protease and food-grade sodium alginate ratio, adds the pure water of 1.2 times of amounts, adjusted
Mixture ph is saved to 4, temperature is maintained at after 75 DEG C, maintain pressure 0.8Mpa to hydrolyze 3 hours, then the hydrolyzate is lost
Living, decolouring and filtration treatment, obtain marine alga polypeptide extract solution；
2. above-mentioned marine alga polypeptide extract solution is purified and concentrated for 1200Da and 500Da NF membrane by molecular weight, obtained
Concentrate；
3. above-mentioned concentrate is passed through into ultra high temperature short time sterilization, be put into pressure pan, be forced into 3kgf/cm2, and keep 10min,
Make its pressure uniform, then pressure is down to Okgf/cm suddenly at 3 seconds2；
4. after sterilizing, algal active peptide powder is obtained by vacuum drying.
The protease include plant rennet and animal protease, protease with plant rennet and animal protease with
1 ︰ 1.2 ratio is mixed.
The plant rennet be subtilopeptidase A and papain, the animal protease be trypsase and
Pepsin.
Embodiment 2：
A kind of preparation method of algal active peptide, comprises the following steps：
1. catalyzing hydrolysis is carried out according to 2 ︰ 1 protease and food-grade sodium alginate ratio, adds the pure water of 1 times of amount, regulation
Mixture ph is to 3, and temperature is maintained at after 50 DEG C, maintains pressure 0.8Mpa to hydrolyze 2 hours, then the hydrolyzate is lost
Living, decolouring and filtration treatment, obtain marine alga polypeptide extract solution；
2. above-mentioned marine alga polypeptide extract solution is purified and concentrated for 1200Da and 500Da NF membrane by molecular weight, obtained
Concentrate；
3. above-mentioned concentrate is passed through into ultra high temperature short time sterilization, be put into pressure pan, be forced into 3kgf/cm2, and keep 20min,
Make its pressure uniform, then pressure is down to Okgf/cm suddenly at 2 seconds2；
4. after sterilizing, algal active peptide powder is obtained by vacuum drying.
The protease include plant rennet and animal protease, protease with plant rennet and animal protease with
1 ︰ 1.5 ratio is mixed.
The plant rennet be subtilopeptidase A and papain, the animal protease be trypsase and
Pepsin.
Embodiment 3：
A kind of preparation method of algal active peptide, comprises the following steps：
1. catalyzing hydrolysis is carried out according to 3 ︰ 1 protease and food-grade sodium alginate ratio, adds the pure water of 2 times of amounts, regulation
Mixture ph is to 3, and temperature is maintained at after 75 DEG C, maintains pressure 0.8Mpa to hydrolyze 3 hours, then the hydrolyzate is lost
Living, decolouring and filtration treatment, obtain marine alga polypeptide extract solution；
2. above-mentioned marine alga polypeptide extract solution is purified and concentrated for 1200Da and 500Da NF membrane by molecular weight, obtained
Concentrate；
3. above-mentioned concentrate is passed through into ultra high temperature short time sterilization, be put into pressure pan, be forced into 3kgf/cm2, and keep 15min,
Make its pressure uniform, then pressure is down to Okgf/cm suddenly at 2 seconds2；
4. after sterilizing, algal active peptide powder is obtained by vacuum drying.
The protease include plant rennet and animal protease, protease with plant rennet and animal protease with
1 ︰ 1.3 ratio is mixed.
The plant rennet be subtilopeptidase A and papain, the animal protease be trypsase and
Pepsin.
Embodiment 4：
A kind of preparation method of algal active peptide, comprises the following steps：
1. catalyzing hydrolysis is carried out according to 4 ︰ 1 protease and food-grade sodium alginate ratio, adds the pure water of 1.5 times of amounts, adjusted
Mixture ph is saved to 4, temperature is maintained at after 70 DEG C, maintain pressure 0.8Mpa to hydrolyze 2.5 hours, then the hydrolyzate is carried out
Inactivation, decolouring and filtration treatment, obtain marine alga polypeptide extract solution；
2. above-mentioned marine alga polypeptide extract solution is purified and concentrated for 1200Da and 500Da NF membrane by molecular weight, obtained
Concentrate；
3. above-mentioned concentrate is passed through into ultra high temperature short time sterilization, be put into pressure pan, be forced into 3kgf/cm2, and keep 15min,
Make its pressure uniform, then pressure is down to Okgf/cm suddenly at 3 seconds2；
4. after sterilizing, algal active peptide powder is obtained by vacuum drying.
The protease include plant rennet and animal protease, protease with plant rennet and animal protease with
1 ︰ 1.4 ratio is mixed.
The plant rennet be subtilopeptidase A and papain, the animal protease be trypsase and
Pepsin.
The present invention has following innovation：
1st, hydrolyzed using complex enzyme coupling and catalyzing, by enzyme dosage, pH value, hydrolysis temperature and hydrolysis time key process parameter
Control, polypeptide molecular weight distribution concentrate；
2nd, filtered using NF membrane two-step method, desalination, one step of concentration are completed, and without desalination and vacuum concentration process, simplify life
Production. art, reduces cost；
3rd, nanofiltration residue and filtrate can be as alga fertilizer raw materials, and for producing alga fertilizer, resource utilization is high；
4th, molecular weight product is in 500~1200Da or so（Mean molecule quantity 800Da）, it is easy to absorption of human body.
Claims (4)
1. a kind of preparation method of algal active peptide, it is characterised in that comprise the following steps：
1. according to（2~5）︰ 1 protease and food-grade sodium alginate ratio carries out catalyzing hydrolysis, and it is pure that 1~2 times of addition is measured
Water, regulation mixture ph is to 3~4, and temperature is maintained at after 50~75 DEG C, maintains pressure 0.8Mpa hydrolysis 2~3 hours, then
The hydrolyzate is inactivated, decolourized and filtration treatment, marine alga polypeptide extract solution is obtained；
2. above-mentioned marine alga polypeptide extract solution is purified and concentrated for 1200Da and 500Da NF membrane by molecular weight, obtained
Concentrate；
3. above-mentioned concentrate is passed through into ultra high temperature short time sterilization, be put into pressure pan, be forced into 3kgf/cm2, and holding 10~
20min, makes its pressure uniform, then pressure is down to Okgf/cm suddenly at 0~3 second2；
4. after sterilizing, algal active peptide powder is obtained by vacuum drying.
2. the preparation method of a kind of algal active peptide according to claim 1, it is characterised in that the protease includes planting
Thing protease and animal protease, protease is with plant rennet and animal protease with 1 ︰（1.2~1.5）Ratio mixing and
Into.
3. a kind of preparation method of algal active peptide according to claim 2, it is characterised in that the plant rennet is
Subtilopeptidase A and papain, the animal protease are trypsase and pepsin.
4. a kind of preparation method of algal active peptide according to claim 1, it is characterised in that the step 1. in, press
Protease and food-grade sodium alginate ratio according to 5 ︰ 1 carry out catalyzing hydrolysis, add the pure water of 1.2 times of amounts, adjust mixture
PH value is to 4, and temperature is maintained at after 75 DEG C, maintains pressure 0.8Mpa to hydrolyze 3 hours.
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| CN201710484878.0A CN107212422A (en) | 2017-06-23 | 2017-06-23 | A kind of preparation method of algal active peptide |
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| CN201710484878.0A CN107212422A (en) | 2017-06-23 | 2017-06-23 | A kind of preparation method of algal active peptide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109527191A (en) * | 2018-11-27 | 2019-03-29 | 北海生巴达生物科技有限公司 | The technique that drink or small molecule Gly-His-Lys are prepared using peptide molecular weight selection technique |
| CN111526721A (en) * | 2017-12-18 | 2020-08-11 | 格玛实验室公司 | Method for identifying and isolating bioactive compounds from seaweed extract |
| CN113278049A (en) * | 2021-05-19 | 2021-08-20 | 国民康健(北京)自然科学研究有限公司 | Selenium-rich flavor peptide separated from selenium-rich seaweed, preparation method and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102228125A (en) * | 2011-05-27 | 2011-11-02 | 湖州佳美生物化学制品有限公司 | Preparation method of algal active peptide |
-
2017
- 2017-06-23 CN CN201710484878.0A patent/CN107212422A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102228125A (en) * | 2011-05-27 | 2011-11-02 | 湖州佳美生物化学制品有限公司 | Preparation method of algal active peptide |
Non-Patent Citations (1)
| Title |
|---|
| 廖小建等，: "来源于海藻抗肿瘤活性环五肽的合成及修饰.", 《中国化学会第十一届全国天然有机化学学术会议论文集》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111526721A (en) * | 2017-12-18 | 2020-08-11 | 格玛实验室公司 | Method for identifying and isolating bioactive compounds from seaweed extract |
| CN109527191A (en) * | 2018-11-27 | 2019-03-29 | 北海生巴达生物科技有限公司 | The technique that drink or small molecule Gly-His-Lys are prepared using peptide molecular weight selection technique |
| CN113278049A (en) * | 2021-05-19 | 2021-08-20 | 国民康健(北京)自然科学研究有限公司 | Selenium-rich flavor peptide separated from selenium-rich seaweed, preparation method and application |
| CN113278049B (en) * | 2021-05-19 | 2022-04-29 | 国民康健(北京)自然科学研究有限公司 | Selenium-rich flavor peptide separated from selenium-rich seaweed, preparation method and application |
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