CN107197873A - A kind of composition pesticide and its application containing chlorine nalidixic bacterium ester and fluazinam - Google Patents
A kind of composition pesticide and its application containing chlorine nalidixic bacterium ester and fluazinam Download PDFInfo
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- CN107197873A CN107197873A CN201710442488.7A CN201710442488A CN107197873A CN 107197873 A CN107197873 A CN 107197873A CN 201710442488 A CN201710442488 A CN 201710442488A CN 107197873 A CN107197873 A CN 107197873A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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Abstract
The invention discloses a kind of composition pesticide containing chlorine nalidixic bacterium ester and fluazinam, comprising active ingredient chlorine nalidixic bacterium ester and fluazinam, the mass ratio of the chlorine nalidixic bacterium ester and fluazinam is 20:1~1:20；The invention also discloses application of the composition pesticide containing chlorine nalidixic bacterium ester and fluazinam in terms of preventing and treating rice blast and Sclerotinia sclerotiorum.The composition pesticide of the present invention alleviates rice blast fungus and Sclerotinia sclerotiorum respectively to chlorine nalidixic bacterium ester and the potential resistance risk of fluazinam, after chlorine nalidixic bacterium ester and fluazinam combination, therebetween there is obvious synergistic effect, effect is notable in terms of preventing and treating rice blast and Sclerotinia sclerotiorum.
Description
Technical field
The present invention relates to a kind of composition pesticide containing chlorine nalidixic bacterium ester and fluazinam and its application, belong to pesticide technology neck
Domain.
Background technology
The rice blast (Rice Blast) as caused by rice blast fungus (Magnaporthe grisea), in China
Rice cultivation area has generation, is that first of the major disease of paddy rice three, current prophylactico-therapeutic measures is mainly disease-resistant variety seed selection, medicament and prevented
Control and cultivation management, wherein, chemical prevention because it is economical, efficient the advantages of and in occupation of irreplaceable status.Due to chemical drugs
Agent it is long-term it is single use, occurred Fungicide Resistance of Pyricularia Oryzae enhancing and pharmacy control efficacy reduce phenomenon, carbendazim
(carbendazim), Prochloraz (prochloraz), Isoprothiolane (Isoprothiolane), kitazine (kitazine), different rice
The report that the net medicament such as (iprobenfos) and kasugarnycin (kasugamycin) of pest has all developed immunity to drugs.Therefore, produce
In be also required to exploitation preventing and treating rice blast new medicament.
Sclerotinia sclerotiorum (Rape caused by Sclerotinia sclerotiorum (Sclerotinia sclerotiorum)
Sclerotinia rot) it is a kind of worldwide distribution disease, there are generation, European spring rape and Asia in each Rape-seed production state
Winter rape morbidity is serious, General Loss 5%~10%, up to 50% when serious.It feels nice and cool and humid region hair relatively in weather
Life is more serious, has generation in all rape producing regions of China, the incidence of disease the most serious with the Yangtze river basin and the southeastern coastal areas
Generally 10%~80%, underproduction loss is 5%~30%, and oil content falls sharply, and influences the yield and quality of rape.20th century 70
The medicament based on benzimidazole germicide carbendazim (Carbendazim, MBC) has been used since age always at the rape florescence
Spraying is prevented and treated.Carbendazim action site is single, single for a long time to use, and causes the distribution of resistant strain to expand year by year,
Resistant strain ratio rises, and causes carbendazim field efficacy to decline.Therefore, the new medicament of exploitation preventing and treating sclerotinia sclerotiorum is to ensureing
The stable high yield of rape is significant.
The content of the invention
Goal of the invention：The problem of existing for rice blast in the prior art and sclerotinia sclerotiorum preventing and treating, the present invention is carried
For a kind of composition pesticide containing chlorine nalidixic bacterium ester and fluazinam；The present invention also provides the composition pesticide in preventing and treating rice blast
With the application in terms of sclerotinia sclerotiorum.
Technical scheme：To achieve these goals, as the present invention provides a kind of agricultural chemicals group containing chlorine nalidixic bacterium ester and fluazinam
Compound, the composition pesticide includes active ingredient chlorine nalidixic bacterium ester and fluazinam, the mass ratio of the chlorine nalidixic bacterium ester and fluazinam
For 20:1~1:20.
It is preferred that, the mass ratio of the active ingredient chlorine nalidixic bacterium ester and fluazinam is 10:1~1:10.
It is preferred that, fludioxonil is also included in the composition pesticide, its consumption is chlorine nalidixic bacterium ester and fluazinam gross mass
0.01-0.05 times.
It is preferred that, auxiliary agent is also included in the composition pesticide.
The auxiliary agent is filler, wetting agent, dispersant, solvent, surfactant, emulsifying agent, stabilizer, thickener, anti-
The one or more frozen in agent, antiprecipitant, defoamer.
The formulation of the composition pesticide is wettable powder (WP), suspending agent (SC), water dispersible granules (WG) or water and milk
Agent (EW).
In the composition pesticide active ingredient chlorine nalidixic bacterium ester and fluazinam in the composition percentage composition be 1%~
90%.
It is of the invention finally to additionally provide the described composition pesticide containing chlorine nalidixic bacterium ester and fluazinam in preventing and treating paddy rice rice blast
Application in terms of disease and sclerotinia sclerotiorum.
Chlorine nalidixic bacterium ester (pyrisoxazole) is the methoxy acrylic sterilization of Shenyang Chemical Engineering Inst's independent research
Agent, medicament disinfecting spectrum is wide, and activity is high, and good to non-target and Environmental compatibility, the lasting period is long, to crop safety, has prevention concurrently
With treatment double activity.Fluazinam is 2,6- dinitroanilines, and resistance of rainwater washing against, the lasting period is long, and its drug effect is better than
A variety of pathogens of anti-benzimidazole type and dicarboximide class biocide agent are also had good effect by conventional protective fungicide
Really.Fluazinam (fluazinam) is 2,6- dinitroanilines, and resistance of rainwater washing against, the lasting period is long, and its drug effect is better than normal
A variety of pathogens of anti-benzimidazole type and dicarboximide class biocide agent are also had good result by the protective fungicide of rule.
In addition, can also include fludioxonil in composition pesticide of the present invention, the raw material is used only the amount of very little, can dramatically increase chlorine pyridine
The prevention effect that bacterium ester and fluazinam are used in combination.
Technique effect：Compared with prior art, the pesticide combination preparation of the invention containing chlorine nalidixic bacterium ester and fluazinam has
Following advantage：
(1) drug-fast generation and development are delayed：The pesticide combination that the present invention is provided is compounded by chlorine nalidixic bacterium ester and fluazinam
Form, can be used with a variety of existing medicament rotations such as the carbendazim in production, thiophanate-methyl and Prochloraz, delay paddy rice
Pyricularia oryzae and Sclerotinia sclerotiorum development drug-fast to existing medicament are with spreading；Alleviate rice blast fungus and oil simultaneously
The potential resistance to the action of a drug of dish hyphal cluster germ respectively to chlorine nalidixic bacterium ester and fluazinam.
(2) medicine synergy is subtracted：It is and many between the two with obvious synergistic effect after chlorine nalidixic bacterium ester and fluazinam compounding
Bacterium spirit, thiophanate-methyl are compared with a variety of existing medicaments such as Prochloraz to be improved while dosage is reduced to rice blast
With the prevention effect of Sclerotinia sclerotiorum.
Embodiment
According to following embodiments, the present invention may be better understood.However, as it will be easily appreciated by one skilled in the art that
Content described by embodiment is merely to illustrate the present invention, without should be also without limitation on described in detail in claims
The present invention.
Embodiment 1
21% chlorine nalidixic bacterium ester fluazinam WP preparation：
By mass percentage, wettable powder each group distribution ratio is：20% chlorine nalidixic bacterium ester, 1% fluazinam, 5% carboxylic first
Base sodium cellulosate (surfactant), 4% pull open powder (wetting agent) supplies surplus with kaolin (filler).
Preparation method：
Got the raw materials ready by said ratio and through conventional wettable powder preparation for processing, prepare 21% chlorine nalidixic bacterium ester fluazinam
WP。
Embodiment 2
21% chlorine nalidixic bacterium ester fluazinam SC preparation：
By mass percentage, suspending agent each component proportioning is：1% chlorine nalidixic bacterium ester, 20% fluazinam, 5% sulfomethylated lignin
Sour sodium (dispersant), 5% alkylnaphthalene sulfonate (wetting agent), 0.1% polyvinyl alcohol (thickener), 0.1% ethylene glycol is (antifreeze
Agent), 0.01% organosilicon (defoamer) supplies surplus with water.
Preparation method：
Two active compound chlorine nalidixic bacterium esters, fluazinam are added to the water, is stirred in stirred tank to homogeneous liquid, adds sulfomethylated lignin
Sour sodium (dispersant), alkylnaphthalene sulfonate (wetting agent) after stirring, is slowly added to use under 80~150rpm mechanical agitations
Polyvinyl alcohol (thickener) after water dilution, ethylene glycol (antifreezing agent), organosilicon (defoamer) supplies 100% content with water；Grind
Liquid material is conveyed using air compression pipe during mill, is continuously sanded using the sand mill of 4 series connection, 21% chlorine pyridine bacterium is made
Ester fluazinam SC.
Embodiment 3
20% chlorine nalidixic bacterium ester fluazinam WP preparation：
By mass percentage, wettable powder each group distribution ratio is：10% chlorine nalidixic bacterium ester, 10% fluazinam, 6% carboxylic first
Base sodium cellulosate (surfactant), 4% pull open powder (wetting agent) supplies surplus with kaolin (filler).
Preparation method：
Got the raw materials ready by said ratio and through conventional wettable powder preparation for processing, prepare 20% chlorine nalidixic bacterium ester fluazinam
WP。
Embodiment 4
15% chlorine nalidixic bacterium ester fluazinam SC preparation：
By mass percentage, suspending agent each component proportioning is：10% chlorine nalidixic bacterium ester, 5% fluazinam, 10% sulfomethylated lignin
Sour sodium (dispersant), 10% alkylnaphthalene sulfonate (wetting agent), 5% polyvinyl alcohol (thickener), 5% ethylene glycol (antifreezing agent),
2% organosilicon (defoamer), surplus is supplied with water.
Preparation method：
Two active compound chlorine nalidixic bacterium esters, fluazinam are added to the water, is stirred in stirred tank to homogeneous liquid, adds sulfomethylated lignin
Sour sodium (dispersant), alkylnaphthalene sulfonate (wetting agent) after stirring, is slowly added to use under 80~150rpm mechanical agitations
Polyvinyl alcohol (thickener) after water dilution, ethylene glycol (antifreezing agent), organosilicon (defoamer) supplies 100% with water；During grinding
Liquid material is conveyed using air compression pipe, is continuously sanded using the sand mill of 4 series connection, 15% chlorine nalidixic bacterium ester fluorine is made
Pyridine amine SC.
Embodiment 5
15% chlorine nalidixic bacterium ester fluazinam SC preparation：
By mass percentage, suspending agent each component proportioning is：5% chlorine nalidixic bacterium ester, 10% fluazinam, 5% sulfomethylated lignin
Sour sodium (dispersant), 5% alkylnaphthalene sulfonate (wetting agent), 0.1% polyvinyl alcohol (thickener), 0.1% ethylene glycol is (antifreeze
Agent), 0.01% organosilicon (defoamer) supplies surplus with water.
Preparation method：
Two active compound chlorine nalidixic bacterium esters, fluazinam are added to the water, is stirred in stirred tank to homogeneous liquid, adds sulfomethylated lignin
Sour sodium (dispersant), alkylnaphthalene sulfonate (wetting agent) after stirring, is slowly added to use under 80~150rpm mechanical agitations
Polyvinyl alcohol (thickener) after water dilution, ethylene glycol (antifreezing agent), organosilicon (defoamer) supplies 100% with water；During grinding
Liquid material is conveyed using air compression pipe, is continuously sanded using the sand mill of 4 series connection, 15% chlorine nalidixic bacterium ester fluorine is made
Pyridine amine SC.
Embodiment 6
11% chlorine nalidixic bacterium ester fluazinam WS preparation：
By mass percentage, wettable powder each group distribution ratio is：1% chlorine nalidixic bacterium ester, 10% fluazinam, 10% alkyl
Phenol polyethenoxy ether (wetting agent), 10% sodium lignin sulfonate (dispersant), 3% sodium carboxymethylcellulose (thickener), 4% second
The organosilicon (defoamer) of glycol (antifreezing agent) 2%, surplus is supplied with water.
Preparation method：
Two active compound chlorine nalidixic bacterium esters, fluazinam are added to the water, is stirred in stirred tank to homogeneous liquid, adds sulfomethylated lignin
Sour sodium (dispersant), APES (wetting agent) delays after stirring under 80~130rpm/min mechanical agitations
Slow sodium carboxymethylcellulose (thickener) ethylene glycol (antifreezing agent) added after being diluted with water, organosilicon (defoamer) is finally used
Water supplies 100% content；Feed liquid is conveyed using air compressed pipe during grinding, is continuously sanded using the sand mill of 4 series connection,
11% chlorine nalidixic bacterium ester fluazinam WS is made.
Embodiment 7
10% chlorine nalidixic bacterium ester fluazinam EW preparation：
By mass percentage, aqueous emulsion each group distribution ratio is：6% chlorine nalidixic bacterium ester, 4% fluazinam, 18% dimethylbenzene (has
Machine solvent), 6% Tween-40 (emulsifying agent), 4% ethylene glycol (antifreezing agent), 1% organosilicon (defoamer) supplies surplus with water.
Preparation method：
First add water in stirred tank, then sequentially add antifreezing agent and defoamer, start high shear mixers mixing
After 20-30 minutes, the oil phase liquid being well mixed by chlorine nalidixic bacterium ester, fluazinam, organic solvent and emulsifying agent is slowly added to, it is high
10% chlorine nalidixic bacterium ester fluazinam EW is made after 1.5 hours in fast stir about.
Embodiment 8
20% chlorine nalidixic bacterium ester fluazinam WP preparation：
By mass percentage, wettable powder each group distribution ratio is：10% chlorine nalidixic bacterium ester, 10% fluazinam, 0.6% coughs up
Bacterium nitrile 0.03 times of fluazinam gross mass (chlorine nalidixic bacterium ester and), 6% sodium carboxymethylcellulose (surfactant), 4% pull open powder
(wetting agent), surplus is supplied with kaolin (filler).
Preparation method：
Got the raw materials ready by said ratio and through conventional wettable powder preparation for processing, prepare 20% chlorine nalidixic bacterium ester fluazinam
WP.The preparation method is similar to Example 3, and difference is in preparation process to add chlorine nalidixic bacterium ester and fluazinam gross mass
0.03 times of fludioxonil.
Embodiment 9
15% chlorine nalidixic bacterium ester fluazinam SC preparation：
By mass percentage, suspending agent each component proportioning is：5% chlorine nalidixic bacterium ester, 10% fluazinam, 0.45% fludioxonil
0.03 times of fluazinam gross mass (chlorine nalidixic bacterium ester and), 5% sodium lignin sulfonate (dispersant), (wetting of 5% alkylnaphthalene sulfonate
Agent), 0.1% polyvinyl alcohol (thickener), 0.1% ethylene glycol (antifreezing agent), 0.01% organosilicon (defoamer) supplies remaining with water
Amount.
Preparation method：
The preparation method is similar to Example 5, and difference is in preparation process to add chlorine nalidixic bacterium ester and fluazinam
The fludioxonil that 0.03 times of gross mass.
Embodiment 10
20% chlorine nalidixic bacterium ester fluazinam WP preparation：It is similar to Example 8, it the difference is that only in preparation process
Add 0.2% fludioxonil 0.01 times of fluazinam gross mass (chlorine nalidixic bacterium ester and).
Embodiment 11
15% chlorine nalidixic bacterium ester fluazinam SC preparation：It is similar to Example 9, it the difference is that only in preparation process
Add 0.75% fludioxonil 0.05 times of fluazinam gross mass (chlorine nalidixic bacterium ester and).
Test example 1
Bacteriostatic activity of the single dose and built agent of chlorine nalidixic bacterium ester and fluazinam to rice blast fungus：
(1) strains tested：Rice blast fungus (Magnaporthe grisea), pick up from Jurong City of Jiangsu Province rice terrace,
Separated by Zhenjiang City,Agriculture Science Research Institute plant protection research department, identify and save backup.Bacterial strain is stored in potato sucrose agar
(PSA) (4 DEG C) on inclined-plane.
(2) reagent agent：95% chlorine nalidixic bacterium ester (Pyrisoxazole) active compound, by Jiangsu Baoling Chemical Co., Ltd
There is provided；97% fluazinam (fluazinam) active compound, is provided by Jiangsu Weier Chemical Co., Ltd.
(3) test method：Reference《Pesticide bioassay test rule NY/T1156.2-2006》, using mycelial growth speed
Rate method carries out the Toxicity Determination of reagent agent single dose and different ratio built agent to rice blast fungus.
(4) data analysis：Using DPS13.0 professional version data handling systems, medicament single dose and different ratio compounding are calculated
Agent is to the growth inhibiting regression equation of rice blast mycelia, EC50And its 95% confidence limit.
According to Wadley methods, coefficient of synergism (SR) is calculated.The synergy of pharmacy mix is evaluated according to coefficient of synergism (SR)
Type, i.e. SR<0.5 is antagonism, and 0.5≤SR≤1.5 are summation action, SR>1.5 be synergistic effect.SR=EC50(Eth)/
EC50(Eob), EC50(Eth)=(a+b)/[(a/EC50A)+(b/EC50B)].Wherein, A, B are respectively medicament single dose, and a, b are phase
Answer ratio of the single dose in mixture, EC50(Eth) it is mixture EC50Theoretical value, EC50(Eob) it is mixture EC50Measured value.
(5) result and analysis：
The chlorine nalidixic bacterium ester of table 1, fluazinam and its built agent are to rice blast fungus bioactivity (virulence) measurement result
Indoor measurement result shows that chlorine nalidixic bacterium ester suppresses the EC of rice blast fungus mycelial growth with fluazinam50Value difference
For 1.4884 and 0.4661 μ g/mL.Chlorine nalidixic bacterium ester and fluazinam compounding, ratio is between 20: 1~1: 20, built agent pair
The coefficient of synergism of rice blast fungus is between 1.50~3.79, with synergistic effect；Ratio is between 10: 1~1: 10
When, the built agent coefficient of synergism antibacterial to rice blast fungus is all higher than 2.0, with more preferable synergistic effect (table 1).Therefore,
Chlorine nalidixic bacterium ester and the optimum ratio of fluazinam compounding are set in 10: 1~1 in preventing and treating rice blast：Between 10.
Test example 2
Bacteriostatic activity of the single dose and built agent of chlorine nalidixic bacterium ester and fluazinam to Sclerotinia sclerotiorum：
(1) strains tested：Sclerotinia sclerotiorum (Sclerotinia sclerotiorum), picks up from China of Jurong City of Jiangsu Province
Positive town Rapeseed Field, is separated by Zhenjiang City,Agriculture Science Research Institute plant protection research department, identifies and save backup.Bacterial strain is stored in horse
On bell potato sucrose agar (PSA) inclined-plane (4 DEG C).
(2) reagent agent：95% chlorine nalidixic bacterium ester (pyrisoxazole) active compound, by Jiangsu Baoling Chemical Co., Ltd
There is provided；97% fluazinam (fluazinam) active compound, is provided by Jiangsu Weier Chemical Co., Ltd.
(3) test method：Reference《Pesticide bioassay test rule NY/T1156.2-2006》, using mycelial growth speed
Rate method carries out the Toxicity Determination of reagent agent single dose and different ratio built agent to Sclerotinia sclerotiorum.
(4) data analysis：Using DPS13.0 professional version data handling systems, medicament single dose and different ratio compounding are calculated
Regression equation that agent suppresses to Sclerotinia sclerotiorum mycelial growth, EC50And its 95% confidence limit.
According to Wadley methods, coefficient of synergism (SR) is calculated.The synergy of pharmacy mix is evaluated according to coefficient of synergism (SR)
Type, i.e. SR<0.5 is antagonism, and 0.5≤SR≤1.5 are summation action, SR>1.5 be synergistic effect.SR=EC50(Eth)/
EC50(Eob), EC50(Eth)=(a+b)/[(a/EC50A)+(b/EC50B)].Wherein, A, B are respectively medicament single dose, and a, b are phase
Answer ratio of the single dose in mixture, EC50(Eth) it is mixture EC50Theoretical value, EC50(Eob) it is mixture EC50Measured value.
(5) result and analysis：
The chlorine nalidixic bacterium ester of table 2, fluazinam and its built agent are to Sclerotinia sclerotiorum bioactivity (virulence) measurement result
Indoor measurement result shows, chlorine nalidixic bacterium ester and fluazinam suppress the EC of Sclerotinia sclerotiorum mycelial growth50Value difference
For 0.0686 and 0.0242 μ g/mL.Chlorine nalidixic bacterium ester and fluazinam compounding, ratio is between 20: 1~1: 20, built agent pair
The coefficient of synergism of rice blast fungus is between 1.21~3.62, with addition or synergistic effect；Ratio is 10: 1~1:
When between 10, the built agent coefficient of synergism antibacterial to Sclerotinia sclerotiorum is all higher than 1.90, and (table 2) is acted on notable synergistic.
Therefore, chlorine nalidixic bacterium ester and the optimum ratio of fluazinam compounding are set in 10: 1~1 in preventing and treating sclerotinia sclerotiorum：Between 10.
Test example 3
By 20% chlorine nalidixic bacterium ester fluazinam WP obtained by embodiment 3, and respectively by chlorine nalidixic bacterium ester and fluazinam by implementation
The method preparation 20% chlorine nalidixic bacterium ester WP and 20% fluazinam WP that example 3 prepares wettable powder does single dose contrast, while with 75%
Tricyclazole WP (Jiangsu Changqing Agricultural Chemistry Co., Ltd.) carries out the field efficacy examination of preventing and treating rice blast as comparison medicament
Test.
Test process method：Test site is located at the agricultural technology renovation of Jiangsu Hilly Ground Zhenjiang Agriculture Science Research Institute
Experimental plot in the garden of center, soil is horse liver soil, middle fertility, the content of organic matter 1.75%.Rice varieties are town rice No. 10,
Field management conventional during experiment.Experiment sets 6 processing, is respectively：20% chlorine nalidixic bacterium ester fluazinam WP 600 and 900g/
hm2, 20% chlorine nalidixic bacterium ester WP 900g/hm2, 20% fluazinam WP 900g/hm2, 75% tricyclazole WP 300g/hm2(recommend agent
Amount) and clear water blank control.Each processing is repeated 3 times, totally 18 cells, and plot area is 30m2, cell set protection row and with
Machine district's groups are arranged.In paddy rice cut phase (on September 5th, 2016), WBD-16 is produced using Zhengzhou Lv Di farm machineries Co., Ltd
Type power spraye, sprays, spouting liquid is 750L/hm in rice leaf and fringe portion2, blank district sprays equivalent clear water.Examination
Other bactericide are no longer sprayed during testing, field management is routinely carried out.In 28d after medicine (on October 3rd, 2016), jumped with parallel
Jump Sample Method Survey spike of rice pest incidence, and each cell investigates 20 caves, and calculates disease index and prevention effect.Disease index
It is respectively with prevention effect calculation formula：Disease index=[Σ (sick spike numbers at different levels × corresponding sick value of series)/total spike number of investigation ×
Highest typical value] × 100；Prevention effect=[(control disease index-processing disease index)/control disease index] ×
100%.
Result of the test shows, 20% chlorine nalidixic bacterium ester fluazinam WP 900g/hm2To the preventive effect highest of rice blast,
20% fluazinam WP 900g/hm2Preventive effect to rice blast is minimum.5 chemicals treatments to the preventive effect of rice blast from high to low
It is followed successively by 20% chlorine nalidixic bacterium ester fluazinam WP 900g/hm2, 20% chlorine nalidixic bacterium ester fluazinam WP 600g/hm2, 20% chlorine pyridine
Bacterium ester WP 900g/hm2, 75% tricyclazole WP 300g/hm2With 20% fluazinam WP 900g/hm2, preventive effect is respectively
84.70%th, 79.54%, 72.26%, 63.67% and 59.57%.20% chlorine nalidixic bacterium ester fluazinam WP 600 and 900g/hm2
Preventive effect to rice blast is above the chlorine nalidixic bacterium ester WP of single dose 20% and 20% fluazinam WP 900g/hm2And comparison medicament 75%
Tricyclazole WP 300g/hm2Preventive effect and significant difference (table 3).This shows the pesticide combination containing chlorine nalidixic bacterium ester and fluazinam to rice
Preferably, also synergy is obvious for field efficacy compared with single dose for the preventive effect of seasonal febrile diseases；Preventive effect when being applied under doses is significantly better than
The preventive effect that conventional dose tricyclazole is applied under recommended dose, the results are shown in Table 3.
Field efficacies of the chlorine nalidixic bacterium ester fluazinam WP of table 3 20% to rice blast
Note：Significant difference (P is represented with different lowercase persons are indicated after column data<0.05).
Test example 4
By 15% chlorine nalidixic bacterium ester fluazinam SC (chlorine nalidixic bacterium esters obtained by embodiment 5:Fluazinam=1:2), and respectively will
The method that chlorine nalidixic bacterium ester and fluazinam prepare suspending agent by embodiment 5 prepares 15% chlorine nalidixic bacterium ester SC and 15% fluazinam SC and makees list
Agent is contrasted, while using 50% carbendazim WP (Zhejiang Xinan Chemical Industry Group Co.Ltd) as comparison medicament, carrying out preventing and treating
The field control effectiveness test of sclerotinia sclerotiorum.
Test process method：Test site is located at the agricultural technology renovation of Jiangsu Hilly Ground Zhenjiang Agriculture Science Research Institute
Experimental plot in the garden of center, soil is horse liver soil, middle fertility, the content of organic matter 1.75%.Rape variety is Qingyou 2,
Field management conventional during experiment.Experiment sets 6 processing, is respectively：15% chlorine nalidixic bacterium ester fluazinam SC 150 and 300g/
hm2, 15% chlorine nalidixic bacterium ester SC 300g/hm2, 15% fluazinam SC 300g/hm2, 50% carbendazim WP 600g/hm2(recommend agent
Amount) and clear water blank control.Each processing is repeated 3 times, totally 18 cells, and plot area is 30m2, cell set protection row and with
Machine district's groups are arranged.In rape flowering, entered using Zhengzhou Lv Di farm machineries Co., Ltd production WBD-16 type power sprayes
Row spraying preventing and treating, spouting liquid is 750L/hm2, blank district sprays equivalent clear water.Other sterilizations are no longer sprayed during experiment
Agent, field management is routinely carried out.Disease is investigated each cell respectively before rape is gathered in one week a situation arises record diseased plant
Number and morbidity series, and calculate disease index and prevention effect.Disease index and prevention effect calculation formula are respectively：The state of an illness
Index=[Σ (diseased plant numbers at different levels × corresponding sick the value of series)/total diseased plant number × superlative degree typical value of investigation] × 100；Prevention effect
=[(control disease index-processing disease index)/control disease index] × 100%.
Result of the test shows, 15% chlorine nalidixic bacterium ester fluazinam SC 300g/hm2To the preventive effect highest of sclerotinia sclerotiorum,
15% fluazinam SC 300g/hm2Preventive effect to sclerotinia sclerotiorum is minimum.5 chemicals treatments are to the preventive effect of sclerotinia sclerotiorum by height
15% chlorine nalidixic bacterium ester fluazinam SC 300g/hm are followed successively by low2, 15% chlorine nalidixic bacterium ester fluazinam SC 150g/hm2, 15%
Chlorine nalidixic bacterium ester SC 300g/hm2, 50% carbendazim WP 600g/hm2With 15% fluazinam SC 300g/hm2, preventive effect is respectively
72.89%th, 83.57%, 53.66%, 45.72% and 38.67%.15% chlorine nalidixic bacterium ester fluazinam SC 150 and 300g/hm2
Preventive effect to sclerotinia sclerotiorum is above the chlorine nalidixic bacterium ester SC of single dose 15% and 15% fluazinam SC and comparison medicament bacterium more than 50%
Clever WP 600g/hm2Preventive effect and significant difference (table 4).This shows the pesticide combination containing chlorine nalidixic bacterium ester and fluazinam to Sclerotina Sclerotiorum in Winter Rape
Preferably, also synergy is obvious for field efficacy compared with single dose for the preventive effect of core disease；Preventive effect when being applied under doses is significantly better than
The preventive effect that conventional dose carbendazim is applied under recommended dose, the results are shown in Table 4.
Field efficacies of the chlorine nalidixic bacterium ester fluazinam SC of table 4 15% to sclerotinia sclerotiorum
Note：Significant difference (P is represented with different lowercase persons are indicated after column data<0.05)..
Test example 5
With test example 3, difference is that the composition pesticide of the present invention applied is embodiment 3 and the gained medicine of embodiment 8
Agent, investigates the difference to rice blast field efficacy after addition auxiliary material polyvinyl alcohol, as a result as follows：
Result of the test shows, 20% chlorine nalidixic bacterium ester fluazinam WP 600g/hm2Preventive effect to rice blast is
75.20%, 0.6% fludioxonil of addition 0.03 times of fluazinam gross mass (chlorine nalidixic bacterium ester and), 20% chlorine nalidixic bacterium ester fluazinam afterwards
WP 600g/hm2Preventive effect to rice blast is 78.11%, both preventive effect significant differences；20% chlorine nalidixic bacterium ester fluazinam
WP 900g/hm2Preventive effect to rice blast is 80.31%, adds 0.6% fludioxonil (chlorine nalidixic bacterium ester and fluazinam gross mass
0.03 times) 20% chlorine nalidixic bacterium ester fluazinam WP 900g/hm afterwards2Preventive effect to rice blast is 84.02%, Liang Zhefang
Imitate difference also notable (table 5).This shows, adds that reagent is supplied after auxiliary material fludioxonil in the pesticide combination of chlorine nalidixic bacterium ester and fluazinam
Agent is combined has synergistic effect to the field efficacy of rice blast.
Field efficacy (drug effect ratios addition fludioxonil after of the chlorine nalidixic bacterium ester fluazinam WP of table 5 20% to rice blast
Compared with)
Note：Significant difference (P is represented with different lowercase persons are indicated after column data<0.05).
Test example 6
With test example 4, difference is that the composition pesticide of the present invention applied is embodiment 5 and the gained medicine of embodiment 9
Agent, investigates the difference to sclerotinia sclerotiorum field efficacy after addition auxiliary material fludioxonil, as a result as follows：
Result of the test shows, 15% chlorine nalidixic bacterium ester fluazinam SC 150g/hm2Preventive effect to sclerotinia sclerotiorum is
71.05%, 0.6% fludioxonil of addition 0.03 times of fluazinam gross mass (chlorine nalidixic bacterium ester and), 15% chlorine nalidixic bacterium ester fluazinam afterwards
SC 150g/hm2Preventive effect to sclerotinia sclerotiorum is 74.37%, both preventive effect significant differences；15% chlorine nalidixic bacterium ester fluazinam
SC 300g/hm2Preventive effect to sclerotinia sclerotiorum is 85.92%, adds 0.6% fludioxonil (chlorine nalidixic bacterium ester and fluazinam gross mass
0.03 times) 15% chlorine nalidixic bacterium ester fluazinam SC 300g/hm afterwards2Preventive effect to sclerotinia sclerotiorum is 89.53%, Liang Zhefang
Imitate difference also notable (table 6).This shows, adds that reagent is supplied after auxiliary material fludioxonil in the pesticide combination of chlorine nalidixic bacterium ester and fluazinam
Agent is combined has synergistic effect to the field efficacy of sclerotinia sclerotiorum.
Field efficacy (drug effect ratios addition fludioxonil after of the chlorine nalidixic bacterium ester fluazinam SC of table 6 15% to sclerotinia sclerotiorum
Compared with)
Note：Significant difference (P is represented with different lowercase persons are indicated after column data<0.05).
Claims (9)
1. a kind of composition pesticide containing chlorine nalidixic bacterium ester and fluazinam, it is characterised in that the composition pesticide include effectively into
Divide chlorine nalidixic bacterium ester and fluazinam, the mass ratio of the chlorine nalidixic bacterium ester and fluazinam is 20:1~1:20.
2. the composition pesticide according to claim 1 containing chlorine nalidixic bacterium ester and fluazinam, it is characterised in that it is described effectively into
The mass ratio for dividing chlorine nalidixic bacterium ester and fluazinam is 10:1~1:10.
3. the composition pesticide according to claim 1 containing chlorine nalidixic bacterium ester and fluazinam, it is characterised in that the agricultural chemicals group
Also include fludioxonil in compound, its consumption is 0.01-0.05 times of chlorine nalidixic bacterium ester and fluazinam gross mass.
4. the composition pesticide according to claim 1 containing chlorine nalidixic bacterium ester and fluazinam, it is characterised in that the agricultural chemicals group
Also include auxiliary agent in compound.
5. the composition pesticide according to claim 4 containing chlorine nalidixic bacterium ester and fluazinam, it is characterised in that the auxiliary agent is
Filler, wetting agent, dispersant, solvent, surfactant, emulsifying agent, stabilizer, thickener, antifreezing agent, antiprecipitant, froth breaking
One or more in agent.
6. the composition pesticide according to claim 1 containing chlorine nalidixic bacterium ester and fluazinam, it is characterised in that the agricultural chemicals group
The formulation of compound is wettable powder (WP), suspending agent (SC), water dispersible granules (WG) or aqueous emulsion (EW).
7. the composition pesticide according to claim 1 containing chlorine nalidixic bacterium ester and fluazinam, it is characterised in that the agricultural chemicals group
Percentage composition is 1%~90% in the composition for active ingredient chlorine nalidixic bacterium ester and fluazinam in compound.
8. the composition pesticide containing chlorine nalidixic bacterium ester and fluazinam described in claim any one of 1-7 is in preventing and treating rice blast side
The application in face.
9. the composition pesticide containing chlorine nalidixic bacterium ester and fluazinam described in claim any one of 1-7 is in preventing and treating sclerotinia sclerotiorum side
The application in face.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103702563A (en) * | 2011-05-25 | 2014-04-02 | 石原产业株式会社 | Agricultural or horticultural fungicide composition and method for controlling plant pathogen |
| CN104115855A (en) * | 2013-04-25 | 2014-10-29 | 陕西美邦农药有限公司 | Efficient sterilization composition containing SYP-7017 |
| CN104430431A (en) * | 2013-09-17 | 2015-03-25 | 南京农业大学 | Composition containing fludioxonil and SYP-7017 |
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2017
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103702563A (en) * | 2011-05-25 | 2014-04-02 | 石原产业株式会社 | Agricultural or horticultural fungicide composition and method for controlling plant pathogen |
| CN104115855A (en) * | 2013-04-25 | 2014-10-29 | 陕西美邦农药有限公司 | Efficient sterilization composition containing SYP-7017 |
| CN104430431A (en) * | 2013-09-17 | 2015-03-25 | 南京农业大学 | Composition containing fludioxonil and SYP-7017 |
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