CN107197759A - A kind of salt tolerant Chinese photinia breeding method - Google Patents
A kind of salt tolerant Chinese photinia breeding method Download PDFInfo
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- CN107197759A CN107197759A CN201710336455.4A CN201710336455A CN107197759A CN 107197759 A CN107197759 A CN 107197759A CN 201710336455 A CN201710336455 A CN 201710336455A CN 107197759 A CN107197759 A CN 107197759A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The present invention relates to agricultural plantation technology field, and in particular to a kind of salt tolerant Chinese photinia breeding method.Specifically：Chinese photinia branch cutting is subjected to culture of rootage in the root media of MS+1.3 1.5mg/L activated carbons, it is placed in progress extraneous root culture in the extraneous root culture medium of MS+4 5mg/L solanines, it is placed in progress strengthening root culture in the strengthening root culture medium obtained by MS+10 12mg/L citrins, it is placed in again in the culture medium obtained by the 0.8mg/L metronidazoles of kelp paste+0.5 and carries out Fiber differentiation, the Chinese photinia plant that the present invention is cultivated has stronger salt-tolerant trait, transplants to salt-soda soil survival rate more than 90.2%.
Description
Technical field
The present invention relates to agricultural plantation technology field, and in particular to a kind of salt tolerant Chinese photinia breeding method.
Background technology
Salt-soda soil is a species of salt aggregation, refers to that the salinity contained by inside soil has influence on the normal growth of crop, salt
Alkali soil salt content it is high, pH value is up to 8-10, and the gas permeability of soil is poor, and structure is poor, and edaphon content is extremely low, in this evil
Under bad soil environment, general green plants are difficult survival, and this undoubtedly gives greening, beautifies the environment and bring very big threat.
Photinia xylophyta, aiphyllium class, rudiment power is strong, resistance to trimming, there is certain resist to flue dust and toxic gas
Property.Rich in ornamental value, it can also make medicinal.The weather of warm and moist is liked, cold hardiness is not strong, enjoy light also resistance to shade, often transplants in fertilizer
The sandy soil of fertile moistening, such as Yangtze river basin and Qinling Mountains areas to the south, Chinese photinia own growth characteristic defines it in varieties in saline-alkali areas
It is difficult to grow survival.
The content of the invention
Cause the problem of greening crop is difficult growth survival to solve salt-soda soil self-defect, it is an object of the invention to provide one
Plant salt tolerant Chinese photinia breeding method.
The technical solution adopted by the present invention is, a kind of salt tolerant Chinese photinia breeding method, specifically：
（1）Choose that Chinese photinia is newborn then, length 3-5cm branch, by branch handstand cuttage in root media, the life
Root culture medium is cultivated 3-5 days by MS+1.3-1.5mg/L activated carbons at dark, forms radical bud of the length in 0.3-0.6cm；
（2）Branch is taken out from root media, cuttage position rinsed well, upright cuttage is in extraneous root culture medium again
In, the extraneous root culture medium is by MS+4-5mg/L solanines；
（3）Branch is taken out from extraneous root culture medium, cuttage position rinsed well, upright cuttage is in strengthening root culture medium again
In, the strengthening root culture medium is by MS+10-12mg/L citrins；
（4）Branch is taken out from strengthening root culture medium, cuttage position rinsed well, upright cuttage is in inducing culture again
In, the inducing culture is cultivated 42-45 hours by kelp paste+0.5-0.8mg/L metronidazoles at dark；
（5）Branch is taken out from inducing culture, cuttage position is rinsed well, upright cuttage again is trained in MS culture mediums
Support 30-35 days, culture is divided into initial stage, mid-term, 3 stages of later stage, salt content is 3-5mg/L in initial stage MS culture mediums, and mid-term is trained
It is 10-12mg/L to support salt content in base, and salt content is 18-20mg/L in later stage culture medium.
It is preferred that, branch handstand cuttage is in root media, and cuttage depth is 0.5-0.8cm.
It is preferred that, it is 1-2 μm with wavelength at 6-8 DEG C, power is 10- when branch cutting is cultivated in extraneous root culture medium
12W infrared irradiation treatment 48-50 hours.
It is preferred that, when branch cutting is cultivated in strengthening root culture medium, at 26-28 DEG C, every 3-5 hours, it is with intensity
5000-5500Lx blue light illumination cuttage branch root, irradiation 18-20 minutes, are carried out continuously 20-22 treatment with irradiation every time.
It is preferred that, branch is taken out from inducing culture, cuttage position is rinsed well, upright cuttage again is trained in MS
Support in base and cultivate 30-35 days, culture is divided into initial stage, mid-term, 3 stages of later stage, salt content is 3-5mg/ in initial stage MS culture mediums
Salt content is 10-12mg/L in L, mid-term culture medium, and salt content is 18-20mg/L in later stage culture medium.
Beneficial effect of the present invention is that the Chinese photinia plant that the present invention is cultivated has stronger salt-tolerant trait, transplants extremely
Salt-soda soil survival rate is more than 90.2%, through being verified in tidal flat of Jiangsu Province salt-soda soil planting experiment, is 8.05%, pH in salt content
It is worth the Chinese photinia plant obtained for 8.5 100 plants of present invention of salt-soda soil definite value, routinely planting patterns is managed, is united after 1 year
Survival rate is counted, survival rate is 95.2%, and average plant height is in 70-82cm.
In cultivating process, a small amount of activated carbon is added in MS culture mediums, the new old generation inside cuttage branch can be promoted
Thank and divide with cambial cell, make cuttage branch Rapid Rooting, same condition of culture phase, compared to the control without activated carbon
Group, cuttage branch generation length is shortened at least 8 days the time required to 0.3-0.6cm radical bud；
In cultivating process, a small amount of solanine is added in MS culture mediums, the mitogenetic of cuttage branch root cell can be activated and divided
Change, make its many raw new roots, same condition of culture phase downwards, compared to the control group without solanine, cuttage branch generates new
Root bar number adds more than 4；
In cultivating process, a small amount of citrin is added in MS culture mediums, strengthening root effect can be played, increase root is thick and root strong
Degree, same condition of culture phase, compared to the control group without citrin, cuttage branch root slightly improves more than 1mm；
By through taking root, the branch that extraneous root, strengthening root culture are obtained is placed in and combines obtained inducing culture by kelp paste and metronidazole
Middle carry out Fiber differentiation, can induce cuttage branch ATP synthesis, promote parenchyma cell division and plasm expansion, can inhale
Substantial amounts of moisture is received and stored, so as to be diluted into internal salt particle, internal salt particle is kept reduced levels, exempts from
Salt damage, improves the salt tolerance of Chinese photinia.
Embodiment
Embodiment 1, a kind of salt tolerant Chinese photinia breeding method, specifically：
（1）Choosing Chinese photinia, newborn, length is in 3-5cm branch then, and by branch handstand cuttage in root media, cuttage is deep
Spend for 0.5cm, the root media is cultivated 3 days by MS+1.3mg/L activated carbons at dark, form length 0.3cm's
Radical bud；
（2）Branch is taken out from root media, cuttage position rinsed well, upright cuttage is in extraneous root culture medium again
In, the extraneous root culture medium is by MS+4mg/L solanines, at 6 DEG C, is 1 μm with wavelength, and power is at 10W infrared light irradiation
Reason 48 hours；
（3）Branch is taken out from extraneous root culture medium, cuttage position rinsed well, upright cuttage is in strengthening root culture medium again
In, the strengthening root culture medium was by MS+10mg/L citrins, at 26 DEG C, every 3 hours, and the blue light for being 5000Lx with intensity shines
Cuttage branch root is penetrated, irradiation 18 minutes, are carried out continuously 20 treatment with irradiation every time；
（4）Branch is taken out from strengthening root culture medium, cuttage position rinsed well, upright cuttage is in inducing culture again
In, the inducing culture is cultivated 42 hours by kelp paste+0.5mg/L metronidazoles at dark；
（5）Branch is taken out from inducing culture, cuttage position is rinsed well, upright cuttage again is trained in MS culture mediums
Support 30 days, culture is divided into initial stage, mid-term, 3 stages of later stage, during salt content is 3mg/L, mid-term culture medium in initial stage MS culture mediums
Salt content is that salt content is 18mg/L in 10mg/L, later stage culture medium.
Embodiment 2, a kind of salt tolerant Chinese photinia breeding method, specifically：
（1）Choosing Chinese photinia, newborn, length is in 3-5cm branch then, and by branch handstand cuttage in root media, cuttage is deep
Spend for 0.8cm, the root media is cultivated 5 days by MS+1.5mg/L activated carbons at dark, form length 0.6cm's
Radical bud；
（2）Branch is taken out from root media, cuttage position rinsed well, upright cuttage is in extraneous root culture medium again
In, the extraneous root culture medium is by MS+5mg/L solanines, at 8 DEG C, is 2 μm with wavelength, and power is at 12W infrared light irradiation
Reason 50 hours；
（3）Branch is taken out from extraneous root culture medium, cuttage position rinsed well, upright cuttage is in strengthening root culture medium again
In, the strengthening root culture medium was by MS+12mg/L citrins, at 28 DEG C, every 5 hours, and the blue light for being 5500Lx with intensity shines
Cuttage branch root is penetrated, irradiation 20 minutes, are carried out continuously 22 treatment with irradiation every time；
（4）Branch is taken out from strengthening root culture medium, cuttage position rinsed well, upright cuttage is in inducing culture again
In, the inducing culture is cultivated 45 hours by kelp paste+0.8mg/L metronidazoles at dark；
（5）Branch is taken out from inducing culture, cuttage position is rinsed well, upright cuttage again is trained in MS culture mediums
Support 35 days, culture is divided into initial stage, mid-term, 3 stages of later stage, during salt content is 5mg/L, mid-term culture medium in initial stage MS culture mediums
Salt content is that salt content is 20mg/L in 12mg/L, later stage culture medium.
Claims (5)
1. a kind of salt tolerant Chinese photinia breeding method, it is characterised in that specific：
（1）Choose that Chinese photinia is newborn then, length 3-5cm branch, by branch handstand cuttage in root media, the life
Root culture medium is cultivated 3-5 days by MS+1.3-1.5mg/L activated carbons at dark, forms radical bud of the length in 0.3-0.6cm；
（2）Branch is taken out from root media, cuttage position rinsed well, upright cuttage is in extraneous root culture medium again
In, the extraneous root culture medium is by MS+4-5mg/L solanines；
（3）Branch is taken out from extraneous root culture medium, cuttage position rinsed well, upright cuttage is in strengthening root culture medium again
In, the strengthening root culture medium is by MS+10-12mg/L citrins；
（4）Branch is taken out from strengthening root culture medium, cuttage position rinsed well, upright cuttage is in inducing culture again
In, the inducing culture is cultivated 42-45 hours by kelp paste+0.5-0.8mg/L metronidazoles at dark；
（5）Branch is taken out from inducing culture, cuttage position is rinsed well, upright cuttage again is trained in MS culture mediums
Support 30-35 days, culture is divided into initial stage, mid-term, 3 stages of later stage, salt content is 3-5mg/L in initial stage MS culture mediums, and mid-term is trained
It is 10-12mg/L to support salt content in base, and salt content is 18-20mg/L in later stage culture medium.
2. a kind of salt tolerant Chinese photinia breeding method according to claim 1, it is characterised in that specific：
Branch handstand cuttage is in root media, and cuttage depth is 0.5-0.8cm.
3. a kind of salt tolerant Chinese photinia breeding method according to claim 1, it is characterised in that specific：
Branch cutting is in extraneous root culture medium, at 6-8 DEG C, is 1-2 μm with wavelength, and power is at 10-12W infrared light irradiation
Reason 48-50 hours.
4. a kind of salt tolerant Chinese photinia breeding method according to claim 1, it is characterised in that specific：
Branch cutting was in strengthening root culture medium, at 26-28 DEG C, every 3-5 hours, the blue light for being 5000-5500Lx with intensity
Cuttage branch root is irradiated, irradiation 18-20 minutes, are carried out continuously 20-22 treatment with irradiation every time.
5. a kind of salt tolerant Chinese photinia breeding method according to claim 1, it is characterised in that specific：
Branch is taken out from inducing culture, cuttage position is rinsed well, upright cuttage again is cultivated in MS culture mediums
30-35 days, culture was divided into initial stage, mid-term, 3 stages of later stage, and salt content is 4mg/L, mid-term culture medium in initial stage MS culture mediums
Middle salt content is 11mg/L, and salt content is 19mg/L in later stage culture medium.
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| CN201710336455.4A CN107197759A (en) | 2017-05-13 | 2017-05-13 | A kind of salt tolerant Chinese photinia breeding method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107548864A (en) * | 2017-10-20 | 2018-01-09 | 北京星河园林景观工程有限公司 | A kind of big tree planting method of beach saline land |
| CN112673958A (en) * | 2020-12-28 | 2021-04-20 | 呼和浩特市科技创新创业服务中心 | Strawberry tissue culture subculture medium and preparation method thereof |
| CN117502019A (en) * | 2023-10-31 | 2024-02-06 | 北京林业大学 | Method for improving mechanical strength of paeonia lactiflora stems |
Citations (5)
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| CN101161055A (en) * | 2007-09-18 | 2008-04-16 | 江苏省农业科学院 | Method for exsomatizing screening grassiness salt resistsomatic mutation body |
| CN101297625A (en) * | 2008-06-17 | 2008-11-05 | 江苏省林业科学研究院 | Cottage method of Photinia glabra leaves |
| CN102440191A (en) * | 2011-10-11 | 2012-05-09 | 山西腾达种业有限公司 | In-vitro culture method for breeding photinia fraseri |
| CN103548685A (en) * | 2013-11-01 | 2014-02-05 | 重庆文理学院 | Rooting culture medium for tissue culture seedling of photinia fraseri as well as in-bottle rooting method and outside-bottle rooting method |
| CN104211512A (en) * | 2014-09-02 | 2014-12-17 | 枞阳县绿洲苗木花卉专业合作社 | Photinia serrulata cuttage culture medium |
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2017
- 2017-05-13 CN CN201710336455.4A patent/CN107197759A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101161055A (en) * | 2007-09-18 | 2008-04-16 | 江苏省农业科学院 | Method for exsomatizing screening grassiness salt resistsomatic mutation body |
| CN101297625A (en) * | 2008-06-17 | 2008-11-05 | 江苏省林业科学研究院 | Cottage method of Photinia glabra leaves |
| CN102440191A (en) * | 2011-10-11 | 2012-05-09 | 山西腾达种业有限公司 | In-vitro culture method for breeding photinia fraseri |
| CN103548685A (en) * | 2013-11-01 | 2014-02-05 | 重庆文理学院 | Rooting culture medium for tissue culture seedling of photinia fraseri as well as in-bottle rooting method and outside-bottle rooting method |
| CN104211512A (en) * | 2014-09-02 | 2014-12-17 | 枞阳县绿洲苗木花卉专业合作社 | Photinia serrulata cuttage culture medium |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107548864A (en) * | 2017-10-20 | 2018-01-09 | 北京星河园林景观工程有限公司 | A kind of big tree planting method of beach saline land |
| CN107548864B (en) * | 2017-10-20 | 2020-02-28 | 北京星河园林景观工程有限公司 | Big tree planting method for coastal saline-alkali soil |
| CN112673958A (en) * | 2020-12-28 | 2021-04-20 | 呼和浩特市科技创新创业服务中心 | Strawberry tissue culture subculture medium and preparation method thereof |
| CN117502019A (en) * | 2023-10-31 | 2024-02-06 | 北京林业大学 | Method for improving mechanical strength of paeonia lactiflora stems |
| CN117502019B (en) * | 2023-10-31 | 2024-09-24 | 北京林业大学 | Method for improving mechanical strength of paeonia lactiflora stems |
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Application publication date: 20170926 |
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