CN107177534A - Novel environment friendly compound probiotic strain of sunshine 518 and preparation method thereof - Google Patents

Novel environment friendly compound probiotic strain of sunshine 518 and preparation method thereof Download PDF

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Publication number
CN107177534A
CN107177534A CN201710519410.0A CN201710519410A CN107177534A CN 107177534 A CN107177534 A CN 107177534A CN 201710519410 A CN201710519410 A CN 201710519410A CN 107177534 A CN107177534 A CN 107177534A
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soil
strain
sunshine
bacteria
environment friendly
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赵龙富
崔建久
孟凡华
丁立孝
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Rizhao Institute Of Bioengineering
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Rizhao Institute Of Bioengineering
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Priority to CN201710519410.0A priority Critical patent/CN107177534A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
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  • Organic Chemistry (AREA)
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  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a kind of novel environment friendly compound probiotic strain of sunshine 518 and preparation method thereof, the prebiotic strain is obtained by the fermenting raw materials of following mass percent:Water purification 85%, probiotics 15%;The probiotics mixes gained for 2.5: 1.5: 1.5: 5.5: 2.5: 1.5: 0.3: 0.3: 0.15: 0.15: 0.1 ratio in mass ratio by photosynthetic bacteria, actinomyces, bacillus, lactic acid bacteria, Lactobacillus plantarum, Bifidobacterium, Rhodopseudomonas palustris, cephalosporium acremonium.The present invention can supplement, develop and grow the profitable strain in soil, produce the chemical substance for killing harmful bacteria, so as to suppress harmful bacteria breeding;Suppress and remove harmful bacteria generation toxin, reduce geobiont pollution, be that plant creates good soil microenvironment, improve diversity of soil microorganism, reduction plant disease occurs, reduction agricultural product heavy-metal residual.

Description

Novel environment friendly compound probiotic strain of sunshine 518 and preparation method thereof
Technical field
The present invention relates to active probiotic strain, and in particular to a kind of novel environment friendly compound probiotic strain of sunshine 518 and Its preparation method.
Background technology
Soil is the material base of the sustainable development of socio-economy, relation body health of people, the beautiful China of relation Build, it is the important content for promoting Ecological Civilization Construction and safeguarding National Ecological Security to protect soil environment.China's arable land soil Earth point position exceeding standard rate is 19.4%, cadmium, nickel, copper, arsenic, mercury, lead, DDT (double rubigan trichloroethanes) and polycyclic aromatic hydrocarbon into For arch-criminal.
China is pesticide producing and uses big country, and the annual land area using agricultural chemicals is used more than 2.8 hundred million hectares Pesticide volume reaches 50~600,000 tons, wherein the agricultural chemicals that there are about 80% is directly entered soil environment and by adsorption by soil, remains in soil In earth.Current China by pollution by pesticides land area more than ten thousand hectares of 1300-1600.Agricultural chemicals turns into the main organic of soil Pollutant.
The annual applying quantity of chemical fertilizer of China is more than 41,000,000 tons.It is long although being the important measures of agricultural volume increase using chemical fertilizer Phase largely can destroy soil texture using nitrogen, the chemical fertilizer such as phosphorus, cause soil hardening, arable soil to degenerate, topsoil shoals, Tilth is deteriorated, water-retention fertilizer ability declines, biological property deteriorates, and adds agriculture production cost, have impact on the yield of crops And quality.
At present, antibiotic and hormone are added in livestock and poultry cultivation, aquaculture feed, so as to promote growth of animal, into For in aquaculture pass through way, in the air and soil around plant, in surface water and groundwater, retail meat and fowl In class, exceeded antibiotic is even there is everywhere in wild animal body, and this is caused greatly to the healthy of people Influence.
Arable soil is the direct supporting body of common people's " rice-sack " and " vegetable basket ", by so serious pollution, Through threatening veterinary antibiotics, the grain these " safety on the tip of the tongue " that we eat daily, perhaps these pollutions are not as chimney In the black smoke, the sewage that drops down in river that emit it is so directly perceived, but they really just with us, people deeply hurt.So controlling Reason soil pollution is extremely urgent, and we, which feel deeply, has a great responsibility.
In summary, because arable land is contaminated, the probiotics in soil is killed, arable soil Tiny ecosystem is destroyed and puts down Weighing apparatus, and then the microecological balance of human body is destroyed, cause body trace element unbalance, cause national chronic disease explosive growth.
The content of the invention
To solve the above problems, the invention provides a kind of novel environment friendly compound probiotic strain of sunshine 518 and its making Method.
To achieve the above object, the technical scheme taken of the present invention is:
A kind of novel environment friendly compound probiotic strain of sunshine 518, the prebiotic strain is sent out by the raw material of following mass percent Obtained by ferment:Water purification 85%, probiotics 15%;The probiotics is by photosynthetic bacteria, actinomyces, bacillus, lactic acid bacteria, the newborn bar of plant Bacterium, Bifidobacterium, Rhodopseudomonas palustris, cephalosporium acremonium are 2.5: 1.5: 1.5: 5.5: 2.5: 1.5: 0.3: 0.3 in mass ratio: 0.15: 0.15: 0.1 ratio mixing gained.
Present invention also offers the preparation method of the above-mentioned novel environment friendly compound probiotic strain of sunshine 518, including following step Suddenly:
S1, by above-mentioned formula weigh each component;
S2, by the water purification weighed using U.S.'s counter-infiltration (RO) filtering technique filtering after, be placed in fermentation tank, add institute Photosynthetic bacteria, actinomyces, bacillus, lactic acid bacteria, Lactobacillus plantarum, Bifidobacterium, Rhodopseudomonas palustris, the top spore weighed It is mould, 25-28 DEG C, ferment 7 to 10 days;
S3, specification canned by 500 milliliters every bottle, 5000 milliliters every barrel or greatly are filling, produce.
The invention has the advantages that:
1), with obtained by the present invention strain fermentation produce series of products planting, animal-breeding, freshwater aquiculture, Sea-farming and daily life use etc. are on probation, and effect is outstanding.10 for many years, on probation in various plants and Tea Production, the production Moral character can be better than EM bacterium, be a kind of " NEW TYPE OF COMPOSITE probiotics ", powerful to soil improvement and defencive function, to tea growth extremely Favorably.
2) profitable strain in tea garden soil, can be supplemented, developed and grown, the chemical substance for killing harmful bacteria is produced, Living space and resource are fought for harmful bacteria, so as to suppress harmful bacteria breeding;Suppress and remove harmful bacteria generation toxin, reduce soil Earth biological pollution, is that plant creates good soil microenvironment, improves diversity of soil microorganism.
3), this product can dilute backlash and apply in plant root, can resist infection of the bacterial virus to plant root and encroach on, can be right Plant support root, take root, mulch, make plant growth vigorous.
4), beneficial bacterium can synthesize the in demand nutriment of a variety of animals and plants to this product during the fermentation, and one is dimension life It is plain, such as:Vitamin A, vitamin K, vitamin B complex etc.;Two be various trace elements, and three be various acid, such as amino acid, core Acid;Four be thousands of remaining kind enzymes;The phenomenon general lack of nutriments such as trace elements in China's soil instantly can effectively be supplemented; The unavailable micro- macromolecular of crops in decomposable asymmetric choice net soil, makes plant be relatively easy to absorption and utilizes micro member Element.
5), the present invention can handle having in farmyard manure, the farmyard manure that can degrade with organic fertilizer compounding application in 1: 100 ratio Malicious harmful substance, improves soil permeability, activating soil, hardened, the sweetening of the soil acid-base value of elimination are native miscellaneous better than being administered alone Fertilizer, effect becomes apparent from.
6), the present invention can kill pathogen, reduce plant disease as farmyard manure leavening;Gold is complexed simultaneously Belong to ion, reduce agricultural product heavy-metal residual.
Embodiment
In order that objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further Describe in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair It is bright.
My company's working condition meets environmental protection microbial inoculum GB20287-2006《Agricultural microbial agent》Standard.Following reality Apply Bifidobacterium Bifidobacterium bifidum, the Lactobacillus plantarum Lactobacillus used in example Plantarun, Rhodopseudomonas palustris Rhodopseudomonas palustris, cephalosporium acremonium Acremonium Chrysogenum, saccharomycete Saccharomyces cerevisiae be purchased from Institute of Microorganism, Academia Sinica subordinate's China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC.Remaining photosynthetic bacteria, actinomyces, bacillus, The strains such as lactic acid bacteria, lactobacillus acidophilus, streptococcus thermophilus are our company oneself collection and seed selection.
Embodiment
A kind of preparation method of the novel environment friendly compound probiotic strain of sunshine 518, comprises the following steps:
S1, weigh by mass percentage:Water purification 85%, probiotics 15%;The probiotics is by photosynthetic bacteria, actinomyces, bud Spore bacillus, lactic acid bacteria, Lactobacillus plantarum, Bifidobacterium, Rhodopseudomonas palustris, cephalosporium acremonium are 2.5: 1.5 in mass ratio: 1.5: 5.5: 2.5: 1.5: 0.3: 0.3: 0.15: 0.15: 0.1 ratio mixing gained.
S2, by the water purification weighed using U.S.'s counter-infiltration (RO) filtering technique filtering after, be placed in fermentation tank, add institute Photosynthetic bacteria, actinomyces, bacillus, lactic acid bacteria, Lactobacillus plantarum, Bifidobacterium, Rhodopseudomonas palustris, the top spore weighed It is mould, 25-28 DEG C, ferment 7 to 10 days;
S3, specification canned by 500 milliliters every bottle, 5000 milliliters every barrel or greatly are filling, produce.
This specific implementation fungus treatment, with bacterium support intestines, with bacterium make corrections gas, the microecological balance in human body is maintained, with bacterium It is exactly to allow beneficial bacterium to be colonized in soil to control bacterium, suppresses breeding of the harmful bacteria in soil, hyperplasia.Soil micro-ecosystem is adjusted to lose Adjust, keep soil micro-ecosystem balance, prevent and treat " root rot ", using biological antagonist principle, reach that " fungus treatment " protects animals and plants Healthy growth.Especially in terms of livestock and poultry cultivation, probiotics instant effect, persistent, without environmental pollution, with antibiolics The incomparable effect of thing, can largely change idea and method that people use antibiotic;Originally it is embodied in Beneficial bacterium can synthesize and be metabolized the in demand nutriment of a variety of animals and plants in fermentation process, and one is various vitamins, such as tie up Raw element K etc.;Two be to have various trace elements;Three be the various acid of synthesis, such as amino acid, nucleic acid;Four be thousands of kinds of enzymes;Can be effective Supplement the phenomenon general lack of nutriments such as trace elements in China's soil instantly.Strain obtained by this specific implementation is to adapt to The high-efficiency strain with region feature of local temperature, humidity and environment, because this strain is compound probiotic strain, its life Life power is very strong, with " environmental protection microbial inoculum " that it ferments, can reach " strain number is more, powerful, solid at every position, flora is prosperous, Fighting capacity is strong, adaptive surface is wide " the features such as.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should It is considered as protection scope of the present invention.

Claims (2)

1. the novel environment friendly compound probiotic strain of sunshine 518, it is characterised in that the prebiotic strain by following mass percent original Material fermentation gained:Water purification 85%, probiotics 15%;The probiotics is by photosynthetic bacteria, actinomyces, bacillus, lactic acid bacteria, plant Lactobacillus, Bifidobacterium, Rhodopseudomonas palustris, cephalosporium acremonium are 2.5: 1.5: 1.5: 5.5: 2.5: 1.5: 0.3 in mass ratio: 0.3: 0.15: 0.15: 0.1 ratio mixing gained.
2. the preparation method of the novel environment friendly compound probiotic strain of sunshine 518, it is characterised in that comprise the following steps:
S1, the formula as described in claim 1 weigh each component;
S2, by the water purification weighed using U.S.'s counter-infiltration (RO) filtering technique filtering after, be placed in fermentation tank, addition weighed Photosynthetic bacteria, actinomyces, bacillus, lactic acid bacteria, Lactobacillus plantarum, Bifidobacterium, Rhodopseudomonas palustris, cephalosporium acremonium, 25-28 DEG C, ferment 7 to 10 days;
S3, specification canned by 500 milliliters every bottle, 5000 milliliters every barrel or greatly are filling, produce.
CN201710519410.0A 2017-06-27 2017-06-27 Novel environment friendly compound probiotic strain of sunshine 518 and preparation method thereof Pending CN107177534A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113444665A (en) * 2021-07-12 2021-09-28 科为博(江苏)生物科技研究院有限公司 Mercury-resistant probiotic compound

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120090365A1 (en) * 2010-10-19 2012-04-19 Holganix Llc Organic Soil Amendments And Method For Enhancing Plant Health
CN103451117A (en) * 2012-06-04 2013-12-18 华中农业大学 Bacillus preparation for preventing and controlling pathogenic bacteria in soil environments as well as preparation method and application thereof
CN103787698A (en) * 2014-02-26 2014-05-14 重庆普天庆能科技有限公司 Novel EM fungicide for composting dung

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120090365A1 (en) * 2010-10-19 2012-04-19 Holganix Llc Organic Soil Amendments And Method For Enhancing Plant Health
CN103451117A (en) * 2012-06-04 2013-12-18 华中农业大学 Bacillus preparation for preventing and controlling pathogenic bacteria in soil environments as well as preparation method and application thereof
CN103787698A (en) * 2014-02-26 2014-05-14 重庆普天庆能科技有限公司 Novel EM fungicide for composting dung

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113444665A (en) * 2021-07-12 2021-09-28 科为博(江苏)生物科技研究院有限公司 Mercury-resistant probiotic compound

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Application publication date: 20170919

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