CN107173648A - It is a kind of for spinach sports drink of fatigue-relieving and preparation method thereof - Google Patents
It is a kind of for spinach sports drink of fatigue-relieving and preparation method thereof Download PDFInfo
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- CN107173648A CN107173648A CN201710424902.1A CN201710424902A CN107173648A CN 107173648 A CN107173648 A CN 107173648A CN 201710424902 A CN201710424902 A CN 201710424902A CN 107173648 A CN107173648 A CN 107173648A
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- 239000008108 microcrystalline cellulose Substances 0.000 description 1
- 229940016286 microcrystalline cellulose Drugs 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 235000014571 nuts Nutrition 0.000 description 1
- 210000004279 orbit Anatomy 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000009928 pasteurization Methods 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 206010036067 polydipsia Diseases 0.000 description 1
- 239000001508 potassium citrate Substances 0.000 description 1
- 229960002635 potassium citrate Drugs 0.000 description 1
- QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 description 1
- 235000011082 potassium citrates Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 235000021251 pulses Nutrition 0.000 description 1
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 230000000391 smoking effect Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000015424 sodium Nutrition 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 1
- 229940048086 sodium pyrophosphate Drugs 0.000 description 1
- 235000014214 soft drink Nutrition 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000012414 sterilization procedure Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000019818 tetrasodium diphosphate Nutrition 0.000 description 1
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 230000004304 visual acuity Effects 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
A kind of spinach sports drink for fatigue-relieving, includes the component of following parts by weight：20 30 parts of extract of spinach；5 15 parts of peanut powder；5 10 parts of almond powder；50 80 parts of white granulated sugar；0.5 1 parts of sodium chloride；0.3 0.5 parts of potassium chloride；0.3 0.5 parts of calcium chloride；0.1 0.2 parts of vitamin C；Vitamin B120.01 0.02 parts；15 parts of citric acid；800 1000 parts of water.The sports drink can supplement protein, and effective fatigue-relieving promotes body recovery.
Description
Technical field
The present invention relates to soft drink industry, and in particular to a kind of for the spinach sports drink of fatigue-relieving and its preparation side
Method.
Background technology
Sports drink is prepared according to the characteristics of physiology is consumed when moving, including certain sugar content, appropriate dimension
Raw element and electrolyte.At present, " red ox ", " screaming " " Jia get Le " etc. are common are on the market, and these sports drinks can supplement people
Energy, sugar, the minerals and vitamins lost after body motion, but the decomposition of metabolin can not be promoted or excreted, fatigue
Remission effect is unsatisfactory, influences the Giving play to physical strength and endurance of human body.
Spinach (Spinacia oleraceaL.) also known as Persian dish, red root vegetables, parrot dish etc., category Chenopodiaceae spinach category, 1 year
Raw herbaceous plant.Plant is up to 1 meter, and root is coniform, redly, it is less for white, leaf universe shape to avette, emerald green, full edge or
There are a small number of tooth shape slivers.Every 1.6~2.9 milligrams of the 100 grams of iron content of spinach, 2.4 grams of protein (equivalent to 2 eggs of 1 jin of spinach
Protein content), 3-Hydroxyretinol milligram (be more than carrot), B10.06 milligram, B20.16 milligram, 31.4 milligrams of C (for kind
3 times of eggplant).The vitamin K lacked in its red containing general vegetables and fruits, contributes to preventing and treating skin, the hemorrhagic tendency of internal organ.Spinach
Dish is also rich in enzyme.The domestic research on utilization to spinach is concentrated mainly on quality and Analysis of Machinability, beverage production process at present
Research and functional component are extracted.CN1015241720 discloses a kind of spinach and asparagus synthetic juice beverage and preparation method thereof.The party
Method cleans spinach, asparagus, and squeeze juice, is mixed, and add water, xylitol etc. in proportion respectively.This method squeezes the juice spinach,
A kind of drink is made, the nutritional ingredient in spinach, but the protein being rich in spinach are can absorb when drinking, is but difficult to absorb profit
With.
There are some researches show some leaf proteins often produce the peptide with physiologically active after hydrolysis.Such as non-patent text
Offer《Antihypertensive Properties of Spinach Leaf Protein Digests》(Yanjun Yang
Deng agricultural and Food Chemistry magazine) a kind of the leaf of spinach active peptides are disclosed, by fresh the leaf of spinach 0.003N sodium hydroxide water
Solution is homogenized；Obtained homogenate is through two panels filtered through gauze, centrifugation；Supernatant liquid filtering, adjusts pH to 4.5 to produce albumen precipitation；
Obtained sediment is used into acetone successively, ethanol and ether are washed on glass filter, until filtrate is colourless；Pepsin-
Pancreatin hydrolysis Spinach Leaf Protein, the pepsin and pancreatin hydrolysate of obtained Spinach Leaf Protein have bright to hypertension mouse
Aobvious blood pressure lowering effect, and Spinach Leaf Protein is then without the effect.
In view of above-mentioned analysis, seeking a kind of spinach sports drink with anti-fatigue active of exploitation turns into the art
Personnel's pursues a goal.
The content of the invention
In order to solve the above-mentioned technical problem, the present invention provides a kind of for the spinach sports drink of fatigue-relieving and its preparation
Method.
To achieve the above object, the present invention takes following technical scheme：
A kind of spinach sports drink for fatigue-relieving, includes the component of following parts by weight：
Preferably,
Wherein, the preparation of the extract of spinach, comprises the following steps：
(1) pre-process：Spinach dish leaf is cleaned, scalding treatment；
(2) it is homogenized：Cooling, is smashed to pieces, add water stirring, and tune pH is 8.5-9.5；
(3) precipitate：Supernatant is collected by filtration, tune pH is 4-5；
(4) digest：Centrifugation, collects precipitation, is dissolved in water, and adds alkalescence-neutral protease enzymolysis, go out enzyme, is filtrated to get
Extract of spinach.
Wherein, 70-100 DEG C of the blanching temperature, time 30s-1.5min.
Wherein, in step 2,45-55 DEG C of the temperature of the stirring that adds water, time 60-90min, solid-liquid ratio (g/ml) is 1:
30-40。
Wherein, in step 3, the sedimentation time is 20-40min.
Wherein, in step 4, after being dissolved in water, precipitating concentration (g/ml) is 2-5%.
Wherein, the alkali protease enzymolysis pH8.0-9.0, time 80-100min, 50-60 DEG C of temperature, enzyme concentration (enzyme-
Substrate is than g/g) it is 1-2%.
Wherein, the neutral protease enzymolysis pH6.8-7.2, time 50-70min, 50-60 DEG C of temperature, enzyme concentration (enzyme-
Substrate is than g/g) it is 1-2%.
Wherein, the enzyme that goes out is that high temperature goes out enzyme or microwave deactivating enzyme.
Further, the stabilizer well known in the art available for sports drink can also be included.The stabilizer is carboxylic
One or more in sodium carboxymethylcellulose pyce, microcrystalline cellulose, starch, gelatin or agar.
The present invention also provides a kind of preparation method of the spinach sports drink for fatigue-relieving, including each raw material is mixed
Close, stir, sterilization filling is produced.
Wherein, sterilize as conventional sterilization procedures, such as pasteurization, microwave sterilization or TRANSIENT HIGH TEMPERATURE sterilizing.
Filtered after above-mentioned enzymolysis, using technology known to those skilled in the art, including filter paper filtering or ultrafiltration.
Above-mentioned extract of spinach can be refined further：Activated carbon is added by 0.5-1% (W/V) in extract of spinach,
80-90 DEG C of stirring of temperature is decolourized, filtering, is repeated to operate above, is obtained light green clear filtrate, freeze-drying or spraying drying powder-forming
End.
In inventive formulation, the reagent for adjusting pH value includes but is not limited to sodium citrate, potassium citrate, phosphoric acid hydrogen two
Sodium, sodium dihydrogen phosphate, sodium pyrophosphate, sodium acid carbonate.
Spinach, is called spinach rib dish, red root vegetables, Persian dish, parrot dish.Spinach contain protein, fat, carbohydrate,
Calcium, phosphorus, iron, vitamin A, vitamin B1, vitamin B2, nicotinic acid, the nutritional ingredient such as vitamin c.
Peanut (peanut), original name peanut (scientific name：Arachis hypogaea Linn.), be China's abundance,
A kind of edible extensive nut, also known as " peanut ", " mud beans " etc..Belong to Rosales, pulse family annual herb plant, stem it is upright or
Crawl, long 30-80 centimetres, ala is separated with fossil fragments, pod is long 2-5 centimetres, wide 1-1.3 centimetres, expansion, pod is thick, flowering fruit bearing stage
The 6-8 months.Peanut fruit contains protein, fat, carbohydrate, vitamin A, vitamin B6, vitamin E, vitamin K, and mineral
The nutritional ingredients such as matter calcium, phosphorus, iron, amino acid and unrighted acid containing 8 kinds of needed by human body, containing lecithin, choline, Hu Luo
The materials such as Bu Su, crude fibre.Fat content is 44%-45%, and protein content is 24-36%, and sugar content is 20% or so.Contain
There is abundant vitamin B2, PP, A, D, E, calcium and iron etc..And contain the multivitamins such as thiamine, riboflavin, niacin.Have
Promote the brain cell development of people, strengthen the effect of memory.
Almond, almond is the seed of rose family apricot.Almond fruit is long on short distance (short spur), with immature outer
See.When fruit maturation, shell of its green can split, and manifest kernel of the bag in coarse shell, kernel be yellow and
There are many duck eyes, shell is hard wooden.Almond is rich in protein, fat, carbohydrate, carrotene, B family vitamin, vitamin
The nutritional ingredient such as C, citrin and calcium, phosphorus, iron.The content of wherein carrotene is only second to mango in fruit, and people are by apricot
Benevolence is referred to as anticancer fruit.Almond contains abundant fat oil, plays the role of to reduce cholesterol.
Technical solution of the present invention, has the following advantages that：
1. the extract of spinach added in the spinach sports drink of the present invention, containing active peptide, excellent aqueous solubility can be supplemented
Protein, effective fatigue-relieving promotes body recovery.
2. in the spinach sports drink of the present invention, the synergistic function between each component effectively plays antifatigue effect.
3. the spinach sports drink of the present invention, bright in colour, with spinach fragrance, it is adaptable to sport people.
Embodiment
Superoxide dismutase determines kit, Mda kit, plasma wrea and determines kit, blood lactase acid measure
Kit, hepatic glycogen determine kit, are purchased from Nanjing Mai Bo bio tech ltd.Swimming trunk, thermostat water bath are purchased from
Beijing Medical Equipment Plant, visible spectrophotometer 754 are purchased from Shanghai precision instrument Co., Ltd, low-temperature and high-speed centrifuge
TDL40B is purchased from Xing Ke scientific ＆ technical corporation；Electronics Libra is purchased from upper Nereid section etc..Male Kunming strain mice is purchased from Jiangxi College of Traditional Chinese Medicine
Institute of lab animals.Peanut powder, almond powder are commercially available.Neutral proteinase (200,000 u/g), alkali protease (200,000
U/g) it is purchased from Nanning Pang Bo bioengineering Co., Ltd.
The preparation of the extract of spinach of embodiment 1
Fresh spinach dish leaf 50g is weighed, is cleaned up.In 500ml beakers, 400ml pure water is added, 80 are heated to
℃.Spinach dish leaf is put into after progress scalding treatment 1min, rapid cooling is taken out.It is put into bruisher and smashs to pieces after cooling, afterwards
In constant temperature blender with magnetic force, 80min is stirred when adding water, amount of water is 1500ml, and temperature adjustment is to 50 DEG C, and it is 9.0 to adjust pH.Through 2-
4 layers of filtered through gauze remove precipitation, and gained supernatant adjusts pH to be 4.5,30min is stirred at room temperature.10min (1500r/min) is centrifuged,
Precipitation is collected, gained is precipitated as spinach protein isolate.Take spinach protein isolate 1g to be placed in 100ml beakers, add 50ml pure water,
It is 8.5 to adjust pH value, and temperature is 55 DEG C, adds basic protein 15mg, acts on 90min；It is 7.0 to adjust pH value, and temperature is 55 DEG C, is added
Neutral proteinase 15mg, acts on 60min, and 90 DEG C of water-bath 10min go out enzyme, filter to obtain extract of spinach.
The preparation of the extract of spinach of embodiment 2
Fresh spinach dish leaf 50g is weighed, is cleaned up.In 500ml beakers, 400ml pure water is added, is heated to
100℃.Spinach dish leaf is put into after progress scalding treatment 30s, rapid cooling is taken out.It is put into bruisher and smashs to pieces after cooling, it
Afterwards in constant temperature blender with magnetic force, 60min is stirred when adding water, amount of water is 2000ml, and temperature adjustment is to 55 DEG C, and it is 8.5 to adjust pH.Through
Layer 2-4 filtered through gauze removes precipitation, and gained supernatant adjusts pH to be 5,20min is stirred at room temperature.10min (1500r/min) is centrifuged,
Precipitation is collected, gained is precipitated as spinach protein isolate.Take spinach protein isolate 1g to be placed in 50ml beakers, add 20ml pure water,
It is 8.0 to adjust pH value, and temperature is 50 DEG C, adds basic protein 20mg, acts on 100min；It is 7.2 to adjust pH value, and temperature is 50 DEG C, plus
Enter neutral proteinase 20mg, act on 70min, 90 DEG C of water-bath 10min go out enzyme.Under the conditions of pressure is 0.7MPa, pass through retention point
Son amount carries out ultrafiltration for 30KD milipore filter, obtains extract of spinach.
The preparation of the extract of spinach of embodiment 3
Fresh spinach dish leaf 50g is weighed, is cleaned up.In 500ml beakers, 400ml pure water is added, 70 are heated to
℃.Spinach dish leaf is put into after progress scalding treatment 1.5min, rapid cooling is taken out.It is put into bruisher and smashs to pieces after cooling, puts
In constant temperature blender with magnetic force, 90min is stirred when adding water, amount of water is 1750ml, and temperature adjustment is to 45 DEG C, and it is 9.5 to adjust pH.Through 2-
4 layers of filtered through gauze remove precipitation, and gained supernatant adjusts pH to be 4,40min is stirred at room temperature.10min (1500r/min) is centrifuged, is received
Collection precipitation, gained is precipitated as spinach protein isolate.Spinach protein isolate 1g is placed in 550ml beakers, 25ml pure water is added, adjusted
PH value is 9.0, and temperature is 60 DEG C, adds basic protein 15mg, acts on 80min；It is 6.8 to adjust pH value, and temperature is 60 DEG C, in addition
Property protease 15mg, act on 50min, 90 DEG C of water-bath 10min go out enzyme, filter to obtain extract of spinach.
Above-mentioned extract of spinach is placed in 4 DEG C of refrigerators and stands 24h, supernatant is taken, 2% Powdered Activated Carbon is added and boils 15min,
Filtering, obtains the light limpid spinach polypeptide solution of color, is freeze-dried into polypeptide powder.
The preparation of the spinach sports drink of embodiment 4
The extract of spinach 24g of the preparation of embodiment 1 is weighed by weight；Peanut powder 10g；Almond powder 7g；White granulated sugar 65g；
Water 900g；Sodium chloride 0.7g；Potassium chloride 0.4g；Calcium chloride 0.4g；Vitamin C 0.15g；Vitamin B120.015g；Citric acid 3g.
Raw material is put into blend tank, heated while stirring uniform, pasteurize is then carried out, filling is finished product.
The preparation of the spinach sports drink of embodiment 5
The extract of spinach 22g of the preparation of embodiment 1 is weighed by weight；Peanut powder 12g；Almond powder 6g；White granulated sugar 60g；
Water 850g；Sodium chloride 0.8g；Potassium chloride 0.35g；Calcium chloride 0.35g；Vitamin C 0.16g；Vitamin B120.012g；Citric acid
2g.Raw material is put into blend tank, heated while stirring uniform, pasteurize is then carried out, filling is finished product.
The preparation of the spinach sports drink of embodiment 6
The extract of spinach 26g of the preparation of embodiment 1 is weighed by weight；Peanut powder 8g；Almond powder 8g；Granulated sugar 70g；Water
950g；Sodium chloride 0.6g；Potassium chloride 0.45g；Calcium chloride 0.45g；Vitamin C 0.12g；Vitamin B120.016g；Citric acid 4g.
Raw material is put into blend tank, heated while stirring uniform, pasteurize is then carried out, filling is finished product.
The preparation of the spinach sports drink of embodiment 7
The extract of spinach 20g of the preparation of embodiment 1 is weighed by weight；Peanut powder 5g；Almond powder 5g；White granulated sugar 50g；Water
1000g；Sodium chloride 0.5g；Potassium chloride 0.3g；Calcium chloride 0.5g；Vitamin C 0.2g；Vitamin B120.01g；Citric acid 1g.
Raw material is put into blend tank, is heated while stirring uniform, then carries out pasteurize, filling is finished product.
The preparation of the spinach sports drink of embodiment 8
The extract of spinach 30g of the preparation of embodiment 1 is weighed by weight；Peanut powder 15g；Almond powder 10g；White granulated sugar 80g；
Water 800g；Sodium chloride 1g；Potassium chloride 0.5g；Calcium chloride 0.3g；Vitamin C 0.1g；Vitamin B120.02g；Citric acid 5g.Original
Material is put into blend tank, is heated while stirring uniform, then carries out pasteurize, filling is finished product.
The preparation of the spinach sports drink of comparative example 1
Similar to Example 4, difference is to be free of peanut powder.
The preparation of the spinach sports drink of comparative example 2
Similar to Example 4, difference is to be free of extract of spinach.
The preparation of the spinach sports drink of comparative example 3
Similar to Example 4, difference is extract of spinach without scalding treatment.
The sensory evaluation scores and Physico-chemical tests of the spinach sports drink of test example 1
(1) sensory evaluation scores
Evaluation group is by 10 member compositions, and each 5 people of men and women, group member is healthy, bad thermophilic without smoking, excessive drinking etc.
It is good, there are stronger resolving power and higher sensitivity to taste.All samples using three random number codes, are carried out at room temperature
Judge.Evaluation criterion is shown in Table 1.Evaluation result is shown in Table 2.
The sensory evaluation standard of the product of table 1
The Analyses Methods for Sensory Evaluation Results of table 2
| Embodiment 4 | Embodiment 5 | Embodiment 6 | Embodiment 7 | Embodiment 8 | Comparative example 1 | Comparative example 1 | Comparative example 3 |
| 98 | 97 | 98 | 98 | 97 | 93 | 85 | 70 |
As can be seen here, spinach is after blanching, and its sensory evaluation score value substantially increases.
(2) detection of extract of spinach
Protein determination：Soluble nitrogen is by Kjeldahl nitrogen determination, specific method reference in protein total nitrogen and supernatant
GB 5009.5-2016 are carried out.The measure of free amine group nitrogen content is according to formaldehyde point position titration.
Nitrogen solubility index：By total nitrogen × 100% in soluble nitrogen in NSI=supernatants/spinacin matter；Degree of hydrolysis DH：Press
DH=(N2-N1)/(N0-N1), in formula, N0：Total nitrogen content g/L；N1：Free Amino Nitrogen content g/L before enzymolysis；N2：After enzymolysis
Free amino nitrogen content g/L.
Purity：The Purity of obtained spinach polypeptide is detected according to forint phenol.
Molecular weight distribution：Micromolecule polypeptide peak molecular weight range is determined using high performance gel filtration chromatography (HPGFC).
The extract of spinach of table 3 is detected
| Embodiment 1 | Embodiment 2 | Embodiment 3 | |
| Nitrogen solubility index NSI (%) | 98.1 | 96.4 | 95.2 |
| Degree of hydrolysis DH | 76.54% | 75.14% | 75.32% |
| Molecular weight distribution | 300-800 | 300-800 | 300-800 |
| Purity % | 55.23 | 58.15 | 60.23 |
The nitrogen solubility index of extract of spinach is up to more than 95%, and alkalescence-neutral proteinase is imitated to the modified of Spinach Leaf Protein
Fruit is preferably；Molecular weight is in 300-800Da；Purity is more than 55%.
The Activity determination of the spinach sports drink of test example 2
(1) experimental animal
Healthy Male Kunming strain mice 120, body weight (20 ± 2) g.Normal, natatory reality will be grown at random by body weight
Test mouse and be divided into 6 groups：Test the spinach sports drink of I group gavages embodiment 4, the spinach motion of experiment II group gavages comparative example 1
Beverage, the spinach sports drink of experiment III group gavages comparative example 2, the spinach sports drink of experiment IV group gavages comparative example 3 is right
According to the group commercially available polypeptide type sports drink of 1 gavage, the gavage physiological saline of control group 2, every group 20, laboratory rearing is filled after 1 week
Stomach is administered, and dosage is 0.1mL10g by body surface area ratio conversion dose,equivalent-1, one time a day, continuous 21d.During experiment,
Mouse freely ingests and drunk water.
(2) measure of mouse growth speed
The body weight of an experiment mice is respectively weighed before and after experiment, calculating averagely has a net increase of quality.It the results are shown in Table 4.
Influence of the different disposal of table 4 to mouse growth speed
Shown from 4,6 groups of mouse stomach 21d result of the tests of table, I groups, II groups, III groups, the mouse of IV groups and CK groups
Averagely having a net increase of quality, there was no significant difference.Therefore spinach sports drink does not influence growing for mouse body.
(3) spinach sports drink safety detection
During 21d is fed, every ingest, drink water, stool and urine situation in 6 groups of mouse of observation, observation are grown, OK
For whether normally, and whether there are poisoning manifestations, test after terminating, the liver of each test mice of inspection, the heart, lung, kidney, jejunum, spleen
Whether dirty, thymus gland and testis sustain damage or lesion.
As a result show, during 21d is fed, the ingesting, drink water of 3 groups of mouse, stool and urine are normal, grow good, not
It was observed that evident act changes and poisoning manifestations, by mouse dissection to its liver, the heart, lung, kidney, jejunum, spleen, chest during off-test
Gland and testis carry out health examination, obvious lesion are not found, each group animal viscera is showed no the damaging as caused by tested material and changed
Become, therefore spinach sports drink is safety non-toxic.
(4) mouse Burden-Swimming Ability of KM is tested
After mouse continuous gavage 21d, last gavage 30min, swimming with a load attached to the body experiment is carried out.Born a heavy burden at mouse tail 1/3
Quality is the sheet lead of 5% body weight, and input depth of water 30cm, the swimming trunk went swimming of 25-30 DEG C of water temperature, observation and record mouse enter certainly
The time that can not be emerged to submerged 10s after water and (exhaust state in power).It the results are shown in Table 5.
Influence of the spinach sports drink of table 5 to the mice burden swimming time
Swimming with a load attached to the body laboratory is the animal model for evaluating antifatigue effect, and length and the exercise tolerance of swimming time have
Close, the degree of animal movement fatigue can be reacted.In this experiment, mouse is given after different nursings, compared with CK, I groups, IV
Group processing can significantly extend the mice burden swimming time, there is nothing between significant difference, but this two groups with II groups, III groups
Significant difference, therefore, it is known that spinach sports drink has certain antifatigue effect.
(5) measure of mouse biochemical indicator
Swimming power is exhausted into rear mouse to pull out from water, the 0.5ml of eye socket blood sampling immediately separates blood breast in serum, detection serum
The content of sour (Lac), superoxide dismutase (SOD), MDA (MDA) and serum urea nitrogen (BUN).
Then experiment mice after blood sampling is put to death immediately.Liver is separated, is blotted after being rinsed through physiological saline with filter paper, accurately
Liver 200mg is weighed, the content of each group mouse hepatic glycogen is determined with anthrone colorimetry.As a result it is as shown in table 6.
The measure of the mouse biochemical indicator of table 6
| Group | Lac% | Hepatic glycogen (mg) | SOD(u/ml) | MDA(mmol/ml) | BUN(mg/ml) |
| I groups | 37.53±1.45 | 5.57±0.67 | 22.55±1.23 | 8.45±0.12 | 14.45±1.01 |
| II groups | 39.79±1.68 | 4.45±0.97 | 20.01±1.74 | 8.93±0.61 | 15.45±1.06 |
| III groups | 42.32±0.38 | 3.83±0.54 | 19.24±1.00 | 9.03±0.13 | 18.40±2.07 |
| IV groups | 37.63±1.43 | 5.45±0.67 | 22.45±1.56 | 8.50±0.44 | 14.34±1.23 |
| CK1 groups | 43.63±1.66 | 3.68±0.67 | 15.27±1.45 | 9.23±0.92 | 19.23±1.12 |
| CK2 groups | 48.31±1.82 | 2.35±0.13 | 12.70±1.36 | 11.43±0.74 | 20.18±1.12 |
As seen from the above table, after to mouse continuous gavage 21d, I groups, the Serum lactic acid content of IV groups, mda content are low, glycogen
Former, superoxide dismutase content is higher, there is significant difference with CK groups.Extract of spinach and peanut powder have good association
Same-action, both are used in conjunction with, and generate synergistic effect.
In motion, muscle generates substantial amounts of lactic acid by glycolysis reaction, and generated hydrogen ion is dissociated by lactic acid
The hydrogen ion concentration in muscle is increased, pH value declines, and then causes a series of Biochemical changes, cause fatigue, reduce lactic acid
Generation or accelerate removings of lactic acid to cause antifatigue effect, MDA is that the sensitivity of measurement matrix Radical Metabolism refers to
Mark, its content can objectively reactive matrix produce free radical level.This is test result indicates that spinach sports drink can suppress fat
Matter peroxidating, reduces lactic acid accumulation, the very fast effect for eliminating sports fatigue.Glycogen is most important energy substance, body in motion
The level of interior glycogen and endurance are closely related, and the raising of glycogen reserve is conducive to raising and the run duration of matrix endurance speed
Extension.Superoxide dismutase oxygen radical excessive in energy removing machine body in tissue, can improve body immunity, strengthen
Oxidation resistance, antioxidant has turned into the important nourishing tonic for sport of a class.In experiment after gavage spinach sports drink, mouse
Superoxide dismutase content, glycogen content are dramatically increased, so as to prove that spinach sports drink has the anti-oxidant of enhancing mouse
Ability, eliminates the function of sports fatigue.Its mechanism is probably that its mechanism may be by hepatic glycogen during increase exhausted movemeat
Deposit, significantly reduce metabolite MDA, BUN level, improve SOD activity, accelerate the removing of free radical, enhancing mouse
Oxidation resistance, mitigates the oxidative damage of matrix, to improve the locomitivity of mouse, plays antifatigue effect.
Obviously, above-described embodiment is only intended to clearly illustrate example, and the not restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change, belong within protection scope of the present invention.There is no necessity and possibility to exhaust all the enbodiments.And thus institute
Among the obvious changes or variations extended out is still in the protection domain of the invention.
Claims (10)
1. a kind of spinach sports drink for fatigue-relieving, it is characterised in that include the component of following parts by weight：
2. the spinach sports drink according to claim 1 for fatigue-relieving, it is characterised in that including following parts by weight
Component：
3. the spinach sports drink according to claim 1 or 2 for fatigue-relieving, it is characterised in that the spinach carries
The preparation of thing is taken to comprise the following steps：
(1) pre-process：Spinach dish leaf is cleaned, scalding treatment；
(2) it is homogenized：Cooling, is smashed to pieces, add water stirring, and tune pH is 8.5-9.5；
(3) precipitate：Supernatant is collected by filtration, tune pH is 4-5；
(4) digest：Centrifugation, collects precipitation, is dissolved in water, and adds alkalescence-neutral protease enzymolysis, go out enzyme, is filtrated to get spinach
Extract.
4. the spinach sports drink according to claim 3 for fatigue-relieving, it is characterised in that the blanching temperature
70-100 DEG C, time 30s-1.5min.
5. the spinach sports drink according to claim 3 for fatigue-relieving, it is characterised in that in step 2, described to add
45-55 DEG C of the temperature of water stirring, time 60-90min, solid-liquid ratio is 1: 30-40.
6. the spinach sports drink according to claim 3 for fatigue-relieving, it is characterised in that in step 3, during precipitation
Between be 20-40min.
7. the spinach sports drink for fatigue-relieving according to any one of claim 3, it is characterised in that in step 4,
After being dissolved in water, precipitating concentration is 2-5%.
8. the spinach sports drink according to claim 3 for fatigue-relieving, it is characterised in that the alkali protease
PH8.0-9.0, time 80-100min are digested, 50-60 DEG C of temperature, enzyme concentration is 1-2%.
9. the spinach sports drink according to claim 3 for fatigue-relieving, it is characterised in that the neutral proteinase
PH6.8-7.2, time 50-70min are digested, 50-60 DEG C of temperature, enzyme concentration is 1-2%.
10. the preparation method of the spinach sports drink for fatigue-relieving described in claim any one of 1-9, its feature exists
In each raw material is mixed, stirred, sterilization filling is produced.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102894249A (en) * | 2012-09-06 | 2013-01-30 | 广东省农业科学院农业生物技术研究所 | Physical fatigue relieving nutrient liquid and preparation method thereof |
| CN103653147A (en) * | 2013-12-20 | 2014-03-26 | 武汉百信正源生物技术工程有限公司 | Wheat peptide anti-fatigue health drink and preparation method thereof |
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2017
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102894249A (en) * | 2012-09-06 | 2013-01-30 | 广东省农业科学院农业生物技术研究所 | Physical fatigue relieving nutrient liquid and preparation method thereof |
| CN103653147A (en) * | 2013-12-20 | 2014-03-26 | 武汉百信正源生物技术工程有限公司 | Wheat peptide anti-fatigue health drink and preparation method thereof |
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| Title |
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| 凌芝: "菠菜冬瓜汁复合饮料的研制", 《饮料工业》 * |
| 梁丽琴，等: "木瓜蛋白酶对菠菜叶蛋白的改性研究", 《食品研究与开发》 * |
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