CN107129980B - 一种饲用蛋白酶制剂保存载体及其制备方法与应用 - Google Patents

一种饲用蛋白酶制剂保存载体及其制备方法与应用 Download PDF

Info

Publication number
CN107129980B
CN107129980B CN201710317552.9A CN201710317552A CN107129980B CN 107129980 B CN107129980 B CN 107129980B CN 201710317552 A CN201710317552 A CN 201710317552A CN 107129980 B CN107129980 B CN 107129980B
Authority
CN
China
Prior art keywords
protease
feed
bentonite
cyclodextrin
beta
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201710317552.9A
Other languages
English (en)
Other versions
CN107129980A (zh
Inventor
黄明媛
张继平
李伟
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yunnan Boshiao Biological Technology Co Ltd
Original Assignee
Yunnan Boshiao Biological Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yunnan Boshiao Biological Technology Co Ltd filed Critical Yunnan Boshiao Biological Technology Co Ltd
Priority to CN201710317552.9A priority Critical patent/CN107129980B/zh
Publication of CN107129980A publication Critical patent/CN107129980A/zh
Application granted granted Critical
Publication of CN107129980B publication Critical patent/CN107129980B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14Enzymes or microbial cells immobilised on or in an inorganic carrier
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/105Aliphatic or alicyclic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/28Silicates, e.g. perlites, zeolites or bentonites
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463Organic compounds; Microorganisms; Enzymes
    • A23L3/3481Organic compounds containing oxygen
    • A23L3/3508Organic compounds containing oxygen containing carboxyl groups
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463Organic compounds; Microorganisms; Enzymes
    • A23L3/3562Sugars; Derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/358Inorganic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A23B - A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/27Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02Enzymes or microbial cells immobilised on or in an organic carrier
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/90Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation

Abstract

本发明公开一种饲用蛋白酶制剂保存载体及其制备方法与应用,属于饲用酶制剂领域。该保存载体包含以下按质量百分比计的组分:滑石粉40%~65%,膨润土20%~30%,二氧化硅3%~10%,玉米芯粉8.9%~15%,β‑环糊精1%~4.7%,无水柠檬酸0.1%~0.3%。能够很好的吸附及释放蛋白酶,且产品经过重压及长期存放不会分层结块,分散均匀,活性持久;在保存液体深层发酵经喷雾干燥的饲用蛋白酶制剂时,具有很好的分散性,对蛋白酶具有充分的保护作用。同时也提高了酶的释放,易于检出且结果准确,降低了检测标准误差。保证了蛋白酶在饲料生产中长期稳定的应用。该方法工艺流程简单,操作方便,易于实施。

Description

一种饲用蛋白酶制剂保存载体及其制备方法与应用
技术领域
本发明属于饲用酶制剂领域,具体涉及一种饲用蛋白酶制剂保存载体及其制备方法与应用。
背景技术
随着科学技术的不断发展,饲用蛋白酶制剂得到了迅速普及,因其能够节约蛋白的作用而被重视,尤其是近年来畜牧行业饲料资源紧张、成本上涨,食品安全问题频发,环境污染日益严重,酶制剂作为一种绿色、安全、无污染的添加剂呈现出越来越明显的价值与作用。蛋白酶作为消化酶主要以复合酶的形式在饲料中使用。然而,饲用蛋白酶作用效果与其活力保存密切相关。
蛋白酶广泛存在于动物、植物和微生物中,饲料工业上应用的蛋白酶目前主要来源于微生物。通过微生物发酵方式获得,目前饲用蛋白酶的生产多采用液体深层发酵方法,经过喷雾干燥技术,制成高浓度酶粉,此发酵方法生产的蛋白酶具有纯度高、污染小、活性强等优点。但是在储存运输过程中容易吸潮结块,酶活力下降速度快,给生产和应用带来了难题,因此急需开发一种能够防结块、酶活保存稳定的饲用蛋白酶保存载体,为饲料生产及畜牧业带来益处。
发明内容
为了克服现有技术的缺点与不足,本发明的首要目的在于提供一种饲用蛋白酶制剂保存载体。该保存载体适用于液体深层发酵喷雾干燥。
本发明的另一目的在于提供上述饲用蛋白酶制剂保存载体的制备方法。该方法工艺流程简单,操作方便,易于实施。
本发明的再一目的在于提供上述饲用蛋白酶制剂保存载体的应用。
本发明的目的通过下述技术方案实现:
一种饲用蛋白酶制剂保存载体,包含以下按质量百分比计的组分:滑石粉40%~65%,膨润土20%~30%,二氧化硅3%~10%,玉米芯粉8.9%~15%,β-环糊精1%~4.7%,无水柠檬酸0.1%~0.3%。
为了更好的实现本发明,
一种饲用蛋白酶制剂保存载体,优选包含以下按质量百分比计的组分:
滑石粉60%,膨润土20%,二氧化硅9%,玉米芯粉8.9%,β-环糊精2%,无水柠檬酸0.1%;
优选的,所述的滑石粉、膨润土、二氧化硅、玉米芯粉、β-环糊精的水分质量含量优选均在10%以下;
更优选的,所述的滑石粉、膨润土、二氧化硅、玉米芯粉、β-环糊精的水分质量含量为:滑石粉3%,膨润土8%,二氧化硅5%,玉米芯粉8%,β-环糊精5%。
所述的饲用蛋白酶制剂保存载体的制备方法,包括以下具体步骤:
(1)将滑石粉、膨润土、二氧化硅、玉米芯粉、β-环糊精和无水柠檬酸分别粉碎,过筛;
(2)按上述比例称取步骤(1)中过筛后的滑石粉、膨润土、二氧化硅、玉米芯粉、β-环糊精和无水柠檬酸,按其添加量从低到高逐级混合,搅拌均匀,制成饲用蛋白酶制剂保存载体。
步骤(1)中所述的粉碎优选是用T130X加强超细磨粉机进行粉碎;
步骤(1)中所述的过筛优选为使用200目筛过筛;
步骤(2)中所述的搅拌均匀优选是用立式KX-500型电动计量干粉搅拌机,在450~500r/min下搅拌10~20min。
所述的饲用蛋白酶制剂保存载体在保存饲用蛋白酶制剂中的应用,优选包含如下步骤:将饲用蛋白酶制剂与保存载体按质量比1:(1~9)复配,制备成蛋白酶活力为10000~150000U/g的饲用蛋白酶。
所述的饲用蛋白酶制剂优选为液体深层发酵经喷雾干燥后的饲用蛋白酶制剂;
所述的饲用蛋白酶制剂优选为中性蛋白酶、酸性蛋白酶和碱性蛋白酶中的至少一种;
所述的饲用蛋白酶制剂的酶活力为100000~300000U/g。
所述的保存载体的复配量要与所要保存的蛋白酶活力比重相当,且保存载体与蛋白酶制剂的配比根据实际生产需要配备。
本发明相对于现有技术,具有如下的优点及效果:
(1)本发明的保存载体原料滑石粉、膨润土、二氧化硅均为无机原料,且含有许多动物生长所需的常量元素和微量元素,均具有较好的吸附和分散能力。其中膨润土具有吸附性、膨胀性、分散性和润滑性,能提高饲料的适口性和改进饲料的松散性,并能延缓饲料通过动物消化道的时间,加强饲料在消化道内的消化吸收作用,并对消化道内的重金属有害气体细菌等有强烈的吸附作用,从而起到对动物的保健作用,由于膨润土是碱性物质,在动物的消化道内能够中和消化道内的酸起到缓冲作用,可增强动物的食欲;
所述保存载体原料β-环糊精可以掩盖或驱除发酵后的蛋白酶产品中的不良异味,提高产品适口性;
所述保存载体原料玉米芯粉具有很好的吸附性和较强的化学稳定性,是优良的有机载体。
所述保存载体原料无水柠檬酸能够有效提高产品稳定性,能提高动物饲料利用率,
(2)本发明饲用蛋白酶制剂保存载体,能够很好的吸附及释放蛋白酶,且产品经过重压及长期存放不会分层结块,分散均匀,活性持久;在保存液体深层发酵经喷雾干燥的饲用蛋白酶制剂时,具有很好的分散性能有效的防止运输及存放过程中的结块问题;对蛋白酶具有充分的保护作用。
(3)本发明饲用蛋白酶制剂保存载体提高了蛋白酶活力稳定性,大大延长了其保质期。解决了现有喷雾干燥后蛋白酶容易吸潮、结块、失活、不易长期保存等问题,并能有效保护蛋白酶直接免受外界环境损伤,保证其活性。同时也提高了酶的释放,易于检出且结果准确,降低了检测标准误差。保证了蛋白酶在饲料生产中长期稳定的应用。
具体实施方式
下面结合实施例对本发明作进一步详细的描述,但本发明的实施方式不限于此。
实施例1
一种饲用蛋白酶制剂保存载体,各成分所占的质量百分比分别为:滑石粉60%,膨润土20%,二氧化硅9%,玉米芯粉8.9%,β-环糊精2%,无水柠檬酸0.1%。各成分其水分含量分别为:滑石粉3%,膨润土8%,二氧化硅5%,玉米芯粉8%,β-环糊精5%。
所述的饲用蛋白酶制剂保存载体的制备方法,包括如下步骤:
(1)将滑石粉、膨润土、二氧化硅、玉米芯粉、β-环糊精和无水柠檬酸分别粉碎,过200目筛,使其细度达到200目;
(2)过200目筛后,按上述配方分别称取各组分,各组分按其添加量从低到高逐级混合,用立式KX-500型电动计量干粉搅拌机,在500r/min下搅拌10min,搅拌均匀,制成饲用蛋白酶制剂保存载体。
实施例2
一种饲用蛋白酶制剂保存载体,各成分所占的质量百分比分别为:滑石粉40%,膨润土30%,二氧化硅10%,玉米芯粉15%,β-环糊精4.7%,无水柠檬酸0.3%。各成分其水分含量分别为:滑石粉3%,膨润土8%,二氧化硅5%,玉米芯粉8%,β-环糊精5%。
所述的饲用蛋白酶制剂保存载体的制备方法,包括如下步骤:
(1)将滑石粉、膨润土、二氧化硅、玉米芯粉、β-环糊精和无水柠檬酸分别粉碎,过200目筛,使其细度达到200目;
(2)过200目筛后,按上述配方分别称取各组分,各组分按其添加量从低到高逐级混合,用立式KX-500型电动计量干粉搅拌机,在480r/min下搅拌15min,搅拌均匀,制成饲用蛋白酶制剂保存载体。
实施例3
一种饲用蛋白酶制剂保存载体,各成分所占的质量百分比分别为:滑石粉65%,膨润土21%,二氧化硅3%,玉米芯粉9.8%,β-环糊精1%,无水柠檬酸0.2%。各成分其水分含量分别为:滑石粉3%,膨润土8%,二氧化硅5%,玉米芯粉8%,β-环糊精5%。
所述的饲用蛋白酶制剂保存载体的制备方法,包括如下步骤:
(1)将滑石粉、膨润土、二氧化硅、玉米芯粉、β-环糊精和无水柠檬酸分别粉碎,过200目筛,使其细度达到200目;
(2)过200目筛后,按上述配方分别称取各组分,各组分按其添加量从低到高逐级混合,用立式KX-500型电动计量干粉搅拌机,在450r/min下搅拌20min,搅拌均匀,制成饲用蛋白酶制剂保存载体。
效果实施例
根据本发明饲用蛋白酶制剂保存载体及制备方法,将此保存载体分别应用于液体深层发酵经喷雾干燥后的酸性蛋白酶、中性蛋白酶及碱性蛋白酶制剂(以上蛋白酶制剂购买自云南博仕奥生物技术有限公司)。根据生产需要将酶活力为100000~300000U/g的饲用蛋白酶制剂与保存载体分别按质量比1:(1~9)复配,制备成酶活力为10000~150000U/g的饲用蛋白酶制剂。定期取样观察并测定酶活力。试验结果表明,使用本发明保存载体的蛋白酶制剂适口性好,无发酵异味,分散性强,不吸潮,不结块,保质期长,活性稳定,室温保存12个月各蛋白酶相对剩余酶活力仍在93%以上。如表1,2,3所示。
表1蛋白酶制剂与实施例1配比后的相对剩余酶活力(%)
表2蛋白酶制剂与实施例2配比后的相对剩余酶活力(%)
表3蛋白酶制剂与实施例3配比后的相对剩余酶活力(%)
上述实施例为本发明较佳的实施方式,但本发明的实施方式并不受上述实施例的限制,其他的任何未背离本发明的精神实质与原理下所作的改变、修饰、替代、组合、简化,均应为等效的置换方式,都包含在本发明的保护范围之内。

Claims (9)

1.一种饲用蛋白酶制剂保存载体,其特征在于包含以下按质量百分比计的组分:滑石粉40%~65%,膨润土20%~30%,二氧化硅3%~10%,玉米芯粉8.9%~15%,β-环糊精1%~4.7%,无水柠檬酸0.1%~0.3%;
所述的滑石粉、膨润土、二氧化硅、玉米芯粉、β-环糊精的水分质量含量均在10%以下。
2.根据权利要求1所述的饲用蛋白酶制剂保存载体,其特征在于:
所述的饲用蛋白酶制剂保存载体,包含以下按质量百分比计的组分:
滑石粉60%,膨润土20%,二氧化硅9%,玉米芯粉8.9%,β-环糊精2%,无水柠檬酸0.1%。
3.根据权利要求1或2所述的饲用蛋白酶制剂保存载体,其特征在于:
所述的滑石粉、膨润土、二氧化硅、玉米芯粉、β-环糊精的水分质量含量为:滑石粉3%,膨润土8%,二氧化硅5%,玉米芯粉8%,β-环糊精5%。
4.权利要求1~3任一项所述的饲用蛋白酶制剂保存载体的制备方法,其特征在于包括以下具体步骤:
(1)将滑石粉、膨润土、二氧化硅、玉米芯粉、β-环糊精和无水柠檬酸分别粉碎,过筛;
(2)按比例称取步骤(1)中过筛后的滑石粉、膨润土、二氧化硅、玉米芯粉、β-环糊精和无水柠檬酸,按其添加量从低到高逐级混合,搅拌均匀,制成饲用蛋白酶制剂保存载体。
5.根据权利要求4所述的饲用蛋白酶制剂保存载体的制备方法,其特征在于:
步骤(1)中所述的过筛为使用200目筛过筛。
6.根据权利要求4所述的饲用蛋白酶制剂保存载体的制备方法,其特征在于:
步骤(2)中所述的搅拌均匀是在450~500r/min下搅拌10~20min。
7.权利要求1~3任一项所述的饲用蛋白酶制剂保存载体在保存饲用蛋白酶制剂中的应用。
8.根据权利要求7所述的应用,其特征在于:
将饲用蛋白酶制剂与权利要求1~4任一项所述的饲用蛋白酶制剂保存载体按质量比1:(1~9)复配,制备成蛋白酶活力为10000~150000U/g的饲用蛋白酶。
9.根据权利要求8所述的应用,其特征在于:
所述的饲用蛋白酶制剂为中性蛋白酶、酸性蛋白酶和碱性蛋白酶中的至少一种;
所述的饲用蛋白酶制剂的酶活力为100000~300000U/g。
CN201710317552.9A 2017-05-08 2017-05-08 一种饲用蛋白酶制剂保存载体及其制备方法与应用 Active CN107129980B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710317552.9A CN107129980B (zh) 2017-05-08 2017-05-08 一种饲用蛋白酶制剂保存载体及其制备方法与应用

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710317552.9A CN107129980B (zh) 2017-05-08 2017-05-08 一种饲用蛋白酶制剂保存载体及其制备方法与应用

Publications (2)

Publication Number Publication Date
CN107129980A CN107129980A (zh) 2017-09-05
CN107129980B true CN107129980B (zh) 2020-01-10

Family

ID=59732205

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710317552.9A Active CN107129980B (zh) 2017-05-08 2017-05-08 一种饲用蛋白酶制剂保存载体及其制备方法与应用

Country Status (1)

Country Link
CN (1) CN107129980B (zh)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107647112B (zh) * 2017-09-18 2021-07-02 江苏瑞牧生物科技有限公司 一种饲用酶制剂载体及其制备方法
CN109043149A (zh) * 2018-07-13 2018-12-21 陕西瑞之源农牧科技有限公司 一种饲料酶制剂用象牙白-200复合颗粒载体的配方及制备方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103459607A (zh) * 2011-03-30 2013-12-18 诺维信公司 酯化反应法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103459607A (zh) * 2011-03-30 2013-12-18 诺维信公司 酯化反应法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
不同载体性质及对植酸酶活性的影响;王冠等;《国外畜牧学》;20101028;第30卷(第5期);第57-59页 *
水产制品除腥技术研究进展;许秀娟等;《中国食物与营养》;20090427(第2期);第32-35页 *
重组有机磷水解酶发酵条件的优化和酶制剂的研究;林恒;《南京农业大学硕士学位论文》;20160329;第57页 *
饲用柠檬酸的应用研究进展;唐茂妍等;《饲料与畜牧》;20150331(第3期);第46-50页 *

Also Published As

Publication number Publication date
CN107129980A (zh) 2017-09-05

Similar Documents

Publication Publication Date Title
CN105623670B (zh) 一种重金属钝化剂及其应用
CN103933595B (zh) 复方除臭剂及其使用方法
CN107129980B (zh) 一种饲用蛋白酶制剂保存载体及其制备方法与应用
CN103073363B (zh) 一种活性全营养多维配方有机肥料及其制造方法
CN106171098A (zh) 一种土壤的改良的方法和改良土壤
CN103539580A (zh) 一种有机无机复合肥及其制备方法
CN108484337A (zh) 一种盐碱土改良剂及其制备方法
CN103319236B (zh) 一种微生物鱼肥及其制备方法
CN108840766A (zh) 一种盐碱土壤改良剂及其制备方法
CN102302087A (zh) 多功能全价厌氧生物发酵饲料
CN105367225B (zh) 一种含固氮菌的生物肥及其制法和应用
CN108587642A (zh) 一种采用煅烧磷石膏的重金属污染盐碱地改良剂
CN103641579A (zh) 一种营养型肥料及其制备方法
CN106699443B (zh) 一种促进含硒旱地土壤中硒活化的调理剂
CN108383661A (zh) 一种土壤改良剂及其制备方法
CN103689287A (zh) 一种微生物鱼肥及其制备方法
CN104276898A (zh) 一种有机肥料及其制备方法
CN103738960A (zh) 一种提高压块活性炭强度的颗粒级配配煤法
CN105949000A (zh) 一种富锌西瓜肥料及其制备方法
CN106995329B (zh) 一种肥料增效剂及使用其的有机肥
CN104447063A (zh) 脲醛炭基复混肥及其间隙式制备方法
CN103555627B (zh) 一种饲用芽孢杆菌活菌制剂保存载体及制备方法与应用
CN103652335A (zh) 鱼体加工副料制作高蛋白颗粒饲料的方法
CN103508810B (zh) 一种纳米碳桉树专用肥以及制备方法
CN110903131A (zh) 一种石墨烯增效的生物纳米硒生物菌肥制备工艺

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant