CN107125696B - 一种鸡肉鲜味肽的分离及其制备方法 - Google Patents
一种鸡肉鲜味肽的分离及其制备方法 Download PDFInfo
- Publication number
- CN107125696B CN107125696B CN201710369337.3A CN201710369337A CN107125696B CN 107125696 B CN107125696 B CN 107125696B CN 201710369337 A CN201710369337 A CN 201710369337A CN 107125696 B CN107125696 B CN 107125696B
- Authority
- CN
- China
- Prior art keywords
- preparation
- enzymolysis
- peptide
- meat
- 1kda
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000019640 taste Nutrition 0.000 title claims abstract description 14
- 238000000926 separation method Methods 0.000 title claims abstract description 7
- 108090000765 processed proteins & peptides Proteins 0.000 title claims description 26
- 238000002360 preparation method Methods 0.000 title claims description 19
- 241000287828 Gallus gallus Species 0.000 title claims description 14
- 206010034203 Pectus Carinatum Diseases 0.000 claims abstract description 12
- 238000004108 freeze drying Methods 0.000 claims abstract description 9
- 108091005804 Peptidases Proteins 0.000 claims abstract description 8
- 239000004365 Protease Substances 0.000 claims abstract description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 8
- 235000013372 meat Nutrition 0.000 claims description 29
- 235000019583 umami taste Nutrition 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 230000002255 enzymatic effect Effects 0.000 claims description 12
- 239000000413 hydrolysate Substances 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 239000006228 supernatant Substances 0.000 claims description 11
- 238000010438 heat treatment Methods 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 7
- 108090000145 Bacillolysin Proteins 0.000 claims description 6
- 241001465754 Metazoa Species 0.000 claims description 6
- 102000035092 Neutral proteases Human genes 0.000 claims description 6
- 108091005507 Neutral proteases Proteins 0.000 claims description 6
- 238000011068 loading method Methods 0.000 claims description 6
- 238000000108 ultra-filtration Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 5
- 230000000415 inactivating effect Effects 0.000 claims description 4
- 229920005654 Sephadex Polymers 0.000 claims description 2
- 239000012507 Sephadex™ Substances 0.000 claims description 2
- 238000013375 chromatographic separation Methods 0.000 claims description 2
- 238000001514 detection method Methods 0.000 claims description 2
- 238000002523 gelfiltration Methods 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 238000012856 packing Methods 0.000 claims description 2
- 230000035945 sensitivity Effects 0.000 claims description 2
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 2
- 239000012498 ultrapure water Substances 0.000 claims description 2
- 238000010829 isocratic elution Methods 0.000 claims 1
- 239000000796 flavoring agent Substances 0.000 abstract description 14
- 235000019634 flavors Nutrition 0.000 abstract description 14
- 101710159104 Flavor peptide Proteins 0.000 abstract description 8
- 238000005516 engineering process Methods 0.000 abstract description 8
- 238000011160 research Methods 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 description 15
- 238000011156 evaluation Methods 0.000 description 14
- 230000001953 sensory effect Effects 0.000 description 12
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 9
- 235000013923 monosodium glutamate Nutrition 0.000 description 9
- 239000004223 monosodium glutamate Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 6
- 239000009705 sanhuang Substances 0.000 description 6
- 230000002779 inactivation Effects 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 238000011161 development Methods 0.000 description 4
- 235000011194 food seasoning agent Nutrition 0.000 description 3
- 238000005227 gel permeation chromatography Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 235000019607 umami taste sensations Nutrition 0.000 description 3
- 239000004278 EU approved seasoning Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000001641 gel filtration chromatography Methods 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000001497 healthy food Nutrition 0.000 description 1
- 235000008446 instant noodles Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013622 meat product Nutrition 0.000 description 1
- 235000012149 noodles Nutrition 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000004537 pulping Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 235000011888 snacks Nutrition 0.000 description 1
- 229940073490 sodium glutamate Drugs 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/20—Synthetic spices, flavouring agents or condiments
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
本发明主要通过蛋白酶靶向酶解技术,结合分离技术、冷冻干燥技术,以三黄鸡胸肉为主要研究对象,得到鲜味较强滋味较柔和的鲜味肽组分,其能够满足市场中鲜味产品的需要。
Description
背景技术:
我国的菜肴多种多样,食品及相应的产品种类丰富,为了制作出鲜香可口、健康的食物需要加入不同的调味料,来提升其味感。目前国内,家庭烹饪及工厂加工工艺主要通过加入味精来增强食品的鲜味,而味精主要成分是谷氨酸钠,食用过多或是使用方法不正确都会对人体造成伤害,破坏人体机能。因此寻找健康安全的调味料来提升食品的鲜味具有重大意义。
肽对食品的滋味有重要贡献,不同的肽因结构不同而呈现出不同的味感,其中鲜味肽或称风味提升肽,是补充或增强食品原有风味的肽类物质,它能提高食品整体的风味,使食品的总体味感协调是复合调味品的重要基料,而肽还具有改善人体机能等生理活性功能。
申请人通过长期的试验发现,将鸡肉酶解、分离、纯化能够得到鲜味明显提升且较柔和的鲜味肽,不仅提升了食物的味感,同时达到了健康安全的目的。
发明内容
本发明的目的是为了增加鲜味肽在食品中的应用逐步减少味精的使用量,利用相对较便宜的鸡胸肉,开发出鲜味强且较柔和的鲜味肽。本发明可以被广泛地应用于菜肴及食品加工中,如:汤类、方便面、米线、膨化休闲食品及肉制品等传统及新兴的工业化产品中。
本发明主要通过蛋白酶靶向酶解技术,结合分离技术、冷冻干燥技术,以三黄鸡胸肉为主要研究对象,得到鲜味较强滋味较柔和的鲜味肽组分,其能够满足市场中鲜味产品的需要。
本发明制备方法如下:
步骤1:酶解液的制备
取三黄鸡鸡胸肉→清洗,去皮→切块打浆→加定量水→预热处理→按比例加入动物蛋白酶→开始酶解一段时间→不灭酶→按比例加入中性蛋白酶酶→酶解→冷却→酶解液成品
具体方法:
将新鲜三黄鸡鸡胸肉去皮切成3-5cm左右的肉片,进绞肉机绞成肉馅,按肉水质量比1.5-3:1加水,在水浴加热下搅拌,待温度达到54℃时,加入0.1%-0.2%动物蛋白酶进行酶解,酶解1.5-3小时,在不灭酶下加入0.25%-0.5%中性蛋白酶,酶解2.5-5小时,酶解结束后,迅速升温至90℃,维持10min灭酶活,然后收集酶解液备用。
鲜味肽组分的制备
三黄鸡鸡胸肉酶解液→离心取上清液→超过滤→感官评价→冷冻干燥→凝胶过滤色谱→感官评价→冷冻干燥→得到鲜味肽组分
具体方法:
将酶解液在4℃条件下,5000r/min离心10min,取上清液,再将上清液按照5KDa、3KDa 和1KDa进行超过滤,得到<1KDa、1-3KDa、3-5KDa和>5KDa4种组分进行冷冻干燥,得到小于1KDa肽的组分鲜味最强。
更优选的是取小于1KDa的组分进行凝胶过滤色谱分离(分离条件为:上样体积为2mL,上样浓度为80mg/mL,玻璃层析柱色谱柱:1.6cm×60cm,柱填料:Sephadex G-15,检测器:紫外检测器,检测波长:220nm,灵敏度:1.0,流动相:超纯水,流速:1mL/min,等梯度洗脱。),收集到4种馏分,通过感官评价,发现第1个流出的馏分M-F-1鲜味明显高于其他3组馏分,其鲜味较强比味精较柔和。
本发明的有益技术效果
研究开发的鲜味肽组分其鲜味强度高,能减少味精的使用,肽还具有保健功能,其在食品中的应用会对人类发展的社会意义较大。
最近国家经济发展一直倡导绿色循环经济,可持续发展策略显得愈发重要。本实验以三黄鸡为原料,开发利用生物酶解技术开发出具有鲜味的肽组分,符合循环经济、综合利用及变废为宝的理念,对环境保护有重要意义。
目前国内外在呈味肽的应用上还没有普及,随着经济不断迅速发展的大背景下,要增强食品加工中科技含量,提高产品的附加值,从而获取高额利润。
所以经本实验研究的鲜味肽组分可以在食品中应用,从而获得高额利润。
附图说明
图1超过滤组分感官评价
图2酶解液(<1KDa)凝胶色谱图
图3酶解液的凝胶色谱组分感官评价
具体的实施方式
下面结合具体实施例对本发明作进一步说明,但不限定本发明的保护范围。
实施例1
鸡肉酶解液鲜味肽组分的制备方法,包括如下步骤:
酶解液的制备
将新鲜三黄鸡鸡胸肉去皮切成3cm左右的肉片,进绞肉机绞成肉馅,按肉水质量比1.5:1 加入水,在水浴加热下搅拌,待温度达到54℃时,加入0.1%动物蛋白酶进行酶解,酶解1.5 小时,在不灭酶下加入0.25%中性蛋白酶,酶解2.5小时,酶解结束后,迅速升温至90℃,维持10min灭酶活,然后收集酶解液备用。
鲜味肽组分的制备
将酶解液在4℃条件下,5000r/min离心10min,取上清液,再将上清液按照5KDa、3KDa 和1KDa进行超过滤,得到<1KDa、1-3KDa、3-5KDa和>5KDa的4种组分进行冷冻干燥。之后对其感官评价(选10名经过感官评价培训的人员,以味精为鲜味评价标准,其浓度为0、2、4、6、8、10、12、14、16、18和20mg/mL对应的鲜味评分值为1、2、3、4、5、6、7、8、 9和10)之后对超过滤组分进行感官评价,其结果如图1和表1所示,得到小于1KDa肽的组分鲜味最强。取小于1KD的组分进行凝胶过滤色谱分离(上样体积为2mL,上样浓度为80mg/mL),如图2所示,得到4种肽的馏分。凝胶色谱分离得到的馏分感官评价结果如图3和表2所示,发现分离出的组分中M-F-1 的鲜味明显高于其他3组分且鲜味较强相比味精较柔和。
表1超过滤组分感官评价表
表2酶解液的凝胶色谱组分感官评价表
实施例2
鸡肉酶解液鲜味肽组分的制备方法,包括如下步骤:
酶解液的制备
将新鲜三黄鸡鸡胸肉去皮切成3cm左右的肉片,进绞肉机绞成肉馅,按肉水质量比1.5:1 加入纯净水,在水浴加热下搅拌,待温度达到54℃时,加入0.1%动物蛋白酶进行酶解,酶解 1.5小时,在不灭酶下加入0.25%中性蛋白酶,酶解2.5小时,酶解结束后,迅速升温至90℃,维持10min灭酶活,然后收集酶解液备用。
鲜味肽组分的制备
将酶解液在4℃条件下,5000r/min离心10min,取上清液,再将上清液按照5KDa、3KDa 和1KDa进行超过滤,得到<1KDa、1-3KDa、3-5KDa和>5KDa 4种组分进行冷冻干燥,之后对其感官评价得到小于1KDa肽的组分鲜味最强。
实施例3
鸡肉酶解液鲜味肽组分的制备方法,包括如下步骤:
酶解液的制备
将新鲜三黄鸡鸡胸肉去皮切成3cm左右的肉片,进绞肉机绞成肉馅,按肉水质量比1.5:1 加入纯净水,在水浴加热下搅拌,待温度达到54℃时,加入0.1%动物蛋白酶进行酶解,酶解 1.5小时,在不灭酶下加入0.25%中性蛋白酶,酶解2.5小时,酶解结束后,迅速升温至90℃,维持10min灭酶活,然后收集酶解液备用。
鲜味肽组分的制备
将酶解液在4℃条件下,5000r/min离心10min,取上清液,再将上清液按照5KDa、3KDa 和1KDa进行超过滤,得到<1KDa、1-3KDa、3-5KDa和>5KDa 4种组分进行冷冻干燥,之后对其感官评价得到小于1KDa肽的组分鲜味最强。
Claims (2)
1.一种鸡肉鲜味肽的制备方法,其特征在于:制备方法如下:
步骤1:酶解液的制备
将新鲜三黄鸡鸡胸肉去皮切成3-5cm的肉片,进绞肉机绞成肉馅,按肉水质量比1.5-3:1加水,在水浴加热下搅拌,待温度达到54℃时,加入0.1%-0.2%动物蛋白酶进行酶解,酶解1.5-3小时,在不灭酶下加入0.25%-0.5%中性蛋白酶,酶解2.5-5小时,酶解结束后,迅速升温至90℃,维持10min灭酶活,然后收集酶解液备用;
步骤2:鲜味肽组分的制备
将酶解液在4℃条件下,5000r/min离心10min,取上清液,再将上清液按照5KDa、3KDa和1KDa进行超过滤,得到<1KDa、1-3KDa、3-5KDa和>5Kda的4种组分进行冷冻干燥,得到小于1KDa鲜味肽,取小于1KDa的组分进行凝胶过滤色谱分离,分离条件为:上样体积为2mL,上样浓度为80mg/mL,玻璃层析柱色谱柱:1.6cm×60cm,柱填料:Sephadex G-15,检测器:紫外检测器,检测波长:220nm,灵敏度:1.0,流动相:超纯水,流速:1mL/min,等梯度洗脱,收集到4种馏分,收集第1个流出的馏分即得鲜味肽。
2.一种鸡肉鲜味肽,其特征在于:按照权利要求1的制备方法制备得到。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710369337.3A CN107125696B (zh) | 2017-05-23 | 2017-05-23 | 一种鸡肉鲜味肽的分离及其制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710369337.3A CN107125696B (zh) | 2017-05-23 | 2017-05-23 | 一种鸡肉鲜味肽的分离及其制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107125696A CN107125696A (zh) | 2017-09-05 |
CN107125696B true CN107125696B (zh) | 2020-09-04 |
Family
ID=59732166
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710369337.3A Active CN107125696B (zh) | 2017-05-23 | 2017-05-23 | 一种鸡肉鲜味肽的分离及其制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107125696B (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107997109A (zh) * | 2017-11-30 | 2018-05-08 | 天津春发生物科技集团有限公司 | 一种基于膜分离技术的酶解鸡肉多肽分级制备方法 |
CN115918881A (zh) * | 2022-05-10 | 2023-04-07 | 山东阜丰发酵有限公司 | 鸡胸肉的酶解工艺以及酶解液在增鲜调味中的应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102125251A (zh) * | 2010-12-09 | 2011-07-20 | 南昌大学 | 可控酶解蛋白制备寡肽鲜味剂的方法 |
CN105707405A (zh) * | 2016-03-15 | 2016-06-29 | 广东厨邦食品有限公司 | 鸡肉鲜味肽及其制备方法与应用 |
-
2017
- 2017-05-23 CN CN201710369337.3A patent/CN107125696B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102125251A (zh) * | 2010-12-09 | 2011-07-20 | 南昌大学 | 可控酶解蛋白制备寡肽鲜味剂的方法 |
CN105707405A (zh) * | 2016-03-15 | 2016-06-29 | 广东厨邦食品有限公司 | 鸡肉鲜味肽及其制备方法与应用 |
Non-Patent Citations (1)
Title |
---|
炖煮鸡汤中多肽与鲜味构效关系;林萌莉等;《食品科学》;20160228;第36卷(第3期);第12-16页 * |
Also Published As
Publication number | Publication date |
---|---|
CN107125696A (zh) | 2017-09-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103549384B (zh) | 一种香辣味香菇酱及其制备方法 | |
KR101219553B1 (ko) | 사골 순대국의 제조 방법 | |
CN103907892A (zh) | 一种纯天然肉味调味料的制备方法 | |
CN107125696B (zh) | 一种鸡肉鲜味肽的分离及其制备方法 | |
CN103005378A (zh) | 一种新型低钠代用盐及其制备方法 | |
CN102887947A (zh) | 以鸭血为原料小分子血肽血红素铁和血浆蛋白的生产工艺 | |
KR100377541B1 (ko) | 굴 소스의 제조방법 | |
KR101079362B1 (ko) | 액젓 조미료 및 그의 제조방법 | |
Kim et al. | β-carotene and retinol contents in Bap, Guk (Tang) and Jjigae of eat-out Korean foods | |
CN105053977A (zh) | 一种含有石斛香辣酱 | |
KR100715148B1 (ko) | 바지락 해장국 | |
KR101236939B1 (ko) | 홍합 소스 및 이의 제조방법 | |
CN1170526A (zh) | 一种羊肉的加工方法 | |
CN105567772B (zh) | 一种高抗氧化性蛋白肽及其制备方法与应用 | |
CN104305135A (zh) | 一种茶树菇保健酱油的制作方法 | |
KR101536433B1 (ko) | 멸치 잔사와 메주를 이용한 어간장 및 어된장 제조방법 및 이에 의해 제조된 어간장 및 어된장 | |
CN102845760B (zh) | 一种鸭血多肽营养肠的制备方法 | |
KR102410059B1 (ko) | 꽃게 껍질을 이용한 천연 조미료의 제조방법 및 이에 따라 제조된 꽃게 껍질 천연 조미료 | |
CN105037494B (zh) | 一种金枪鱼暗色肉蛋白抗氧化铁螯合肽的用途 | |
JP2001299290A (ja) | 褐色ソース | |
KR101146151B1 (ko) | 대두 가수분해물을 함유한 항고혈압 기능성 무침용 천연조미료 및 그의 제조방법 | |
KR101380495B1 (ko) | 패류를 이용한 농축 액상조미료의 제조방법과 그에 의해 제조된 액상조미료 | |
KR20210055994A (ko) | 참치 추출액 소스 및 제조 방법 | |
Fang et al. | Effects of different protease treatment on protein degradation and flavor components of Lentinus edodes | |
CN105265939A (zh) | 一种蟹足佐餐食品及其加工方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240219 Address after: No. 168 Chengdong Avenue, Yichang City, Hubei Province 443000 (Formerly No. 24 Zhongnan Road) Patentee after: ANGELYEAST Co.,Ltd. Country or region after: China Address before: 100037, Fu Cheng Road, Beijing, Haidian District, No. 33 Patentee before: BEIJING TECHNOLOGY AND BUSINESS University Country or region before: China |