CN107125021A - A kind of preparation method containing polliniferous lucidum strain - Google Patents
A kind of preparation method containing polliniferous lucidum strain Download PDFInfo
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- CN107125021A CN107125021A CN201710281067.0A CN201710281067A CN107125021A CN 107125021 A CN107125021 A CN 107125021A CN 201710281067 A CN201710281067 A CN 201710281067A CN 107125021 A CN107125021 A CN 107125021A
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- wood chip
- wheat bran
- white sugar
- rape pollen
- broken wall
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of preparation method containing polliniferous lucidum strain, comprise the following steps that：Wood chip, wheat bran, land plaster, white sugar, water and rape pollen are got out as raw material；Rape pollen is dried successively, sieves, irradiate, broken wall treatment obtains broken wall rape pollen；Batch mixing, wood chip is spread out and uniformly sprinkles wheat bran, land plaster, and white sugar is put into 35 times of water and melted, then broken wall rape pollen is melted into the syrup modulated, and syrup is uniformly sprinkling upon to wood chip mixture surface；While pouring waterside rolling, it is sufficiently mixed raw material；Cultivated after sterilizing, inoculation.The present invention is not on the basis of cultivation of glossy ganoderma strain main raw material(s) is changed, by adding rape pollen, strengthen bacterial activity and rate of vaccination, it compensate for the not enough shortcoming of the nutriments such as culture medium growth factor and mineral matter, strengthen bacterial activity, and then improve ganoderma lucidum product Yield and quality, with it is economical and practical, simple to operate, effect is notable the features such as.
Description
Technical field
The present invention relates to a kind of strain, specifically a kind of preparation method containing polliniferous lucidum strain.
Background technology
Edible mushroom is the edible macro fungi of the mankind, and China is vast in territory, and ABUNDANT NATUREAL RESOURSES possesses quantity various
Wild edible mushroom resources and long edible mushroom eat history.Development and maturation with modern age edible mushroom artificial cultivation technique,
China has become Edible Fungi state and exported country maximum in the world.
The health Chinese medicine that ganoderma lucidum is praised highly as China, Asia or even the world, from the sixties in last century, ganoderma lucidum people's work post
Planting technology and planting number are significantly improved, while ganoderma lucidum product also sells to the countries such as Korea Spro, day, America and Europe extensively.
At present, research finds that edible mushroom grows, mainly by carbon source, nitrogen source and inorganic mineral, grow because
Son, water are used as nutriment.And ganoderma lucidum is as saprophytic mushroom, its original seed, cultigen primary raw material by wood chip, land plaster, sugar,
Water is constituted, and the raw material has price excellent, and purchase is convenient, the advantages of being easy to operation, because wheat bran is compared with other nitrogen sources,
Nitrogen content is higher but vitamin is not enough, and the shortage of especially B family vitamin can have a strong impact on growing for strain, and then influence
Yield and quality.
Research finds to be rich in several amino acids, vitamin A, vitamin C, vitamin E, active matter in the pollen in nature
The composition such as matter and trace elements of selenium, phosphatide, nucleic acid, be described as be nature assign the mankind high-quality nutrient source, more than 200
Plant nutritional ingredient.
It is found by experiment that, the processing of special process is carried out to rape pollen, culture medium can be made up as additive
The deficiency of nutriment, significantly improves lucidum strain activity and rate of vaccination, reaches the effect of ganoderma lucidum good quality and high output.
The content of the invention
It is an object of the invention to provide a kind of economical and practical, simple to operate preparation side containing polliniferous lucidum strain
Method, to solve the problems mentioned in the above background technology.
To achieve the above object, the present invention provides following technical scheme：
A kind of preparation method containing polliniferous lucidum strain, is comprised the following steps that：
（1）Wood chip, wheat bran, land plaster, white sugar, water and rape pollen are got out as raw material；The wood chip, wheat bran, gypsum
Powder, the ratio of white sugar are followed successively by 76-80：18-20:1:1；
（2）Rape pollen is dried successively, sieves, irradiate, broken wall treatment obtains broken wall rape pollen；
（3）Batch mixing, wood chip is spread out and uniformly sprinkles wheat bran, land plaster, white sugar is put into 3-5 times of water and melted, then by broken wall
Rape pollen melts into the syrup modulated, and syrup is uniformly sprinkling upon to wood chip mixture surface；While pouring waterside rolling, make former material
Material is sufficiently mixed；The addition of the broken wall rape pollen is per ton to add according to the mixture of wood chip, wheat bran, land plaster, white sugar
Plus 3-5g；
（4）Sterilizing, is loaded into the raw material of cultivation bag, is sterilized according to conventional edible mushroom method；
（5）Inoculation, in aseptic operating platform, strain is accessed into bacterium bag；
（6）Culture, by the bacterium bag after inoculation, cultivates to Ganoderma lucidum mycelium under conditions of 25-30 DEG C of room temperature, humidity 60-65% and covers with
Surface and inside.
It is used as further scheme of the invention：The wood chip, wheat bran, land plaster, the ratio of white sugar are followed successively by 78：19:1:
1。
It is used as further scheme of the invention：The wood chip is broad-leaved class hardwood wood chip.
It is used as further scheme of the invention：The addition of the broken wall rape pollen is according to wood chip, wheat bran, gypsum
The mixture addition 4g per ton of powder, white sugar.
Compared with prior art, the beneficial effects of the invention are as follows：
The present invention is rich by the appropriate cheap, nutrition of addition on the basis of cultivation of glossy ganoderma strain main raw material(s) is not changed
Rich rape pollen, enhancing bacterial activity and rate of vaccination, compensate for the nutriments such as culture medium growth factor and mineral matter not enough
Shortcoming, strengthen bacterial activity, and then improve ganoderma lucidum product Yield and quality, it is notable with economical and practical, simple to operate, effect
The features such as.
Embodiment
The technical scheme of this patent is described in more detail with reference to embodiment.
Embodiment 1
A kind of preparation method containing polliniferous lucidum strain, is comprised the following steps that：
（1）Wood chip, wheat bran, land plaster, white sugar, water and rape pollen are got out as raw material；The wood chip, wheat bran, gypsum
Powder, the ratio of white sugar are followed successively by 76：18:1:1；The wood chip is broad-leaved class hardwood wood chip；
（2）Rape pollen is dried successively, sieves, irradiate, broken wall treatment obtains broken wall rape pollen；
（3）Batch mixing, wood chip is spread out and uniformly sprinkles wheat bran, land plaster, and white sugar is put into 3 times of water and melted, then broken wall is oily
Cauliflower powder is melted into the syrup modulated, and syrup is uniformly sprinkling upon to wood chip mixture surface；While pouring waterside rolling, make raw material
It is sufficiently mixed；To hold wood chip, just not water outlet is advisable.The addition of the broken wall rape pollen is according to wood chip, wheat bran, stone
The mixture addition 3g per ton of cream powder, white sugar；
（4）Sterilizing, is loaded into the raw material of cultivation bag, is sterilized according to conventional edible mushroom method；
（5）Inoculation, in aseptic operating platform, strain is accessed into bacterium bag；
（6）Culture, by the bacterium bag after inoculation, cultivated under conditions of 25 DEG C of room temperature, humidity 60% to Ganoderma lucidum mycelium cover with surface and
It is internal.
Embodiment 2
A kind of preparation method containing polliniferous lucidum strain, is comprised the following steps that：
（1）Wood chip, wheat bran, land plaster, white sugar, water and rape pollen are got out as raw material；The wood chip, wheat bran, gypsum
Powder, the ratio of white sugar are followed successively by 77：18.5:1:1；The wood chip is broad-leaved class hardwood wood chip；
（2）Rape pollen is dried successively, sieves, irradiate, broken wall treatment obtains broken wall rape pollen；
（3）Batch mixing, wood chip is spread out and uniformly sprinkles wheat bran, land plaster, and white sugar is put into 3.5 times of water and melted, then by broken wall
Rape pollen melts into the syrup modulated, and syrup is uniformly sprinkling upon to wood chip mixture surface；While pouring waterside rolling, make former material
Material is sufficiently mixed；To hold wood chip, just not water outlet is advisable.The addition of the broken wall rape pollen be according to wood chip, wheat bran,
The mixture addition 3.5g per ton of land plaster, white sugar；
（4）Sterilizing, is loaded into the raw material of cultivation bag, is sterilized according to conventional edible mushroom method；
（5）Inoculation, in aseptic operating platform, strain is accessed into bacterium bag；
（6）Culture, by the bacterium bag after inoculation, cultivated under conditions of 26 DEG C of room temperature, humidity 61% to Ganoderma lucidum mycelium cover with surface and
It is internal.
Embodiment 3
A kind of preparation method containing polliniferous lucidum strain, is comprised the following steps that：
（1）Wood chip, wheat bran, land plaster, white sugar, water and rape pollen are got out as raw material；The wood chip, wheat bran, gypsum
Powder, the ratio of white sugar are followed successively by 78：19:1:1；The wood chip is broad-leaved class hardwood wood chip；
（2）Rape pollen is dried successively, sieves, irradiate, broken wall treatment obtains broken wall rape pollen；
（3）Batch mixing, wood chip is spread out and uniformly sprinkles wheat bran, land plaster, and white sugar is put into 4 times of water and melted, then broken wall is oily
Cauliflower powder is melted into the syrup modulated, and syrup is uniformly sprinkling upon to wood chip mixture surface；While pouring waterside rolling, make raw material
It is sufficiently mixed；To hold wood chip, just not water outlet is advisable.The addition of the broken wall rape pollen is according to wood chip, wheat bran, stone
The mixture addition 4g per ton of cream powder, white sugar；
（4）Sterilizing, is loaded into the raw material of cultivation bag, is sterilized according to conventional edible mushroom method；
（5）Inoculation, in aseptic operating platform, strain is accessed into bacterium bag；
（6）Culture, by the bacterium bag after inoculation, cultivated under conditions of 28 DEG C of room temperature, humidity 62% to Ganoderma lucidum mycelium cover with surface and
It is internal.
Embodiment 2
A kind of preparation method containing polliniferous lucidum strain, is comprised the following steps that：
（1）Wood chip, wheat bran, land plaster, white sugar, water and rape pollen are got out as raw material；The wood chip, wheat bran, gypsum
Powder, the ratio of white sugar are followed successively by 79：19.5:1:1；The wood chip is broad-leaved class hardwood wood chip；
（2）Rape pollen is dried successively, sieves, irradiate, broken wall treatment obtains broken wall rape pollen；
（3）Batch mixing, wood chip is spread out and uniformly sprinkles wheat bran, land plaster, and white sugar is put into 4.5 times of water and melted, then by broken wall
Rape pollen melts into the syrup modulated, and syrup is uniformly sprinkling upon to wood chip mixture surface；While pouring waterside rolling, make former material
Material is sufficiently mixed；To hold wood chip, just not water outlet is advisable.The addition of the broken wall rape pollen be according to wood chip, wheat bran,
The mixture addition 4.5g per ton of land plaster, white sugar；
（4）Sterilizing, is loaded into the raw material of cultivation bag, is sterilized according to conventional edible mushroom method；
（5）Inoculation, in aseptic operating platform, strain is accessed into bacterium bag；
（6）Culture, by the bacterium bag after inoculation, cultivated under conditions of 29 DEG C of room temperature, humidity 64% to Ganoderma lucidum mycelium cover with surface and
It is internal.
Embodiment 5
A kind of preparation method containing polliniferous lucidum strain, is comprised the following steps that：
（1）Wood chip, wheat bran, land plaster, white sugar, water and rape pollen are got out as raw material；The wood chip, wheat bran, gypsum
Powder, the ratio of white sugar are followed successively by 80：20:1:1；The wood chip is broad-leaved class hardwood wood chip；
（2）Rape pollen is dried successively, sieves, irradiate, broken wall treatment obtains broken wall rape pollen；
（3）Batch mixing, wood chip is spread out and uniformly sprinkles wheat bran, land plaster, and white sugar is put into 5 times of water and melted, then broken wall is oily
Cauliflower powder is melted into the syrup modulated, and syrup is uniformly sprinkling upon to wood chip mixture surface；While pouring waterside rolling, make raw material
It is sufficiently mixed；To hold wood chip, just not water outlet is advisable.The addition of the broken wall rape pollen is according to wood chip, wheat bran, stone
The mixture addition 5g per ton of cream powder, white sugar；
（4）Sterilizing, is loaded into the raw material of cultivation bag, is sterilized according to conventional edible mushroom method；
（5）Inoculation, in aseptic operating platform, strain is accessed into bacterium bag；
（6）Culture, by the bacterium bag after inoculation, cultivated under conditions of 30 DEG C of room temperature, humidity 65% to Ganoderma lucidum mycelium cover with surface and
It is internal.
The present invention is subjected to planting experiment with Common ganoderma lucidum bacterium culture medium, concrete outcome is as shown in table 1：
The lucidum strain culture medium of table 1 is contrasted
| Project | Bacteria developing period | Success ratio of inoculation | Ganoderma lucidum yield | Spore powder yield |
| Ordinary culture medium | It is longer | Typically | Typically | Typically |
| This product culture medium | Fast 5 days | Improve 50% | Improve 10% | Improve 5% |
Drawn, added in sawdust medium by the contrast of above-mentioned planting experiment（Rape）Pollen is production high-quality original seed and cultigen
Effective way, and to improve ganoderma lucidum plantation income, have significance.
The present invention passes through the appropriate cheap, battalion of addition on the basis of cultivation of glossy ganoderma strain main raw material(s) is not changed
Abundant rape pollen, enhancing bacterial activity and rate of vaccination are supported, the nutriments such as culture medium growth factor and mineral matter are compensate for
Not enough shortcoming, strengthens bacterial activity, and then improves ganoderma lucidum product Yield and quality, with economical and practical, simple to operate, effect
Significantly the features such as.
The better embodiment to this patent is explained in detail above, but this patent is not limited to above-mentioned embodiment party
, can also be on the premise of this patent objective not be departed from formula, the knowledge that one skilled in the relevant art possesses
Various changes can be made.
Claims (4)
1. a kind of preparation method containing polliniferous lucidum strain, it is characterised in that comprise the following steps that：
（1）Wood chip, wheat bran, land plaster, white sugar, water and rape pollen are got out as raw material；The wood chip, wheat bran, gypsum
Powder, the ratio of white sugar are followed successively by 76-80：18-20:1:1；
（2）Rape pollen is dried successively, sieves, irradiate, broken wall treatment obtains broken wall rape pollen；
（3）Batch mixing, wood chip is spread out and uniformly sprinkles wheat bran, land plaster, white sugar is put into 3-5 times of water and melted, then by broken wall
Rape pollen melts into the syrup modulated, and syrup is uniformly sprinkling upon to wood chip mixture surface；While pouring waterside rolling, make former material
Material is sufficiently mixed；The addition of the broken wall rape pollen is per ton to add according to the mixture of wood chip, wheat bran, land plaster, white sugar
Plus 3-5g；
（4）Sterilizing, is loaded into the raw material of cultivation bag, is sterilized according to conventional edible mushroom method；
（5）Inoculation, in aseptic operating platform, strain is accessed into bacterium bag；
（6）Culture, by the bacterium bag after inoculation, cultivates to Ganoderma lucidum mycelium under conditions of 25-30 DEG C of room temperature, humidity 60-65% and covers with
Surface and inside.
2. the preparation method according to claim 1 containing polliniferous lucidum strain, it is characterised in that the wood chip, wheat
Bran, land plaster, the ratio of white sugar are followed successively by 78：19:1:1.
3. the preparation method according to claim 1 containing polliniferous lucidum strain, it is characterised in that the wood chip is wealthy
Leaf class hardwood wood chip.
4. the preparation method according to claim 1 containing polliniferous lucidum strain, it is characterised in that the broken wall rape
The addition of pollen be according to wood chip, wheat bran, land plaster, white sugar mixture addition 4g per ton.
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| CN201710281067.0A CN107125021A (en) | 2017-04-26 | 2017-04-26 | A kind of preparation method containing polliniferous lucidum strain |
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| CN201710281067.0A CN107125021A (en) | 2017-04-26 | 2017-04-26 | A kind of preparation method containing polliniferous lucidum strain |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1233389A (en) * | 1998-04-28 | 1999-11-03 | 山东省滨州卫生学校 | Method for cultivating selenium-rich glossy ganoderma |
| CN101273693A (en) * | 2008-04-09 | 2008-10-01 | 河源市天仙湖农业发展有限公司 | Method for cultivating Ganoderma Sinense |
| CN101411287A (en) * | 2008-11-25 | 2009-04-22 | 福建省农业科学院农业生态研究所 | Method for cultivating Ganoderma lucidum using bamboo tube |
| CN102674929A (en) * | 2012-05-12 | 2012-09-19 | 福建华尔康生物科技有限公司 | Inonotus obliquus submerged fermentation culture medium and submerged fermentation method thereof |
| CN103508798A (en) * | 2013-09-27 | 2014-01-15 | 合肥市天丰菌业科技有限公司 | Enokitake cultivation material using silkworm excrement as raw material and preparation method thereof |
| CN104663250A (en) * | 2015-03-12 | 2015-06-03 | 象州县科学技术局 | Method for cultivating phoenix mushroom with mulberry twigs |
-
2017
- 2017-04-26 CN CN201710281067.0A patent/CN107125021A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1233389A (en) * | 1998-04-28 | 1999-11-03 | 山东省滨州卫生学校 | Method for cultivating selenium-rich glossy ganoderma |
| CN101273693A (en) * | 2008-04-09 | 2008-10-01 | 河源市天仙湖农业发展有限公司 | Method for cultivating Ganoderma Sinense |
| CN101411287A (en) * | 2008-11-25 | 2009-04-22 | 福建省农业科学院农业生态研究所 | Method for cultivating Ganoderma lucidum using bamboo tube |
| CN102674929A (en) * | 2012-05-12 | 2012-09-19 | 福建华尔康生物科技有限公司 | Inonotus obliquus submerged fermentation culture medium and submerged fermentation method thereof |
| CN103508798A (en) * | 2013-09-27 | 2014-01-15 | 合肥市天丰菌业科技有限公司 | Enokitake cultivation material using silkworm excrement as raw material and preparation method thereof |
| CN104663250A (en) * | 2015-03-12 | 2015-06-03 | 象州县科学技术局 | Method for cultivating phoenix mushroom with mulberry twigs |
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Application publication date: 20170905 |