CN107124888A - 鼓泡状接头元件和使用其构建测序文库的方法 - Google Patents

鼓泡状接头元件和使用其构建测序文库的方法 Download PDF

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CN107124888A
CN107124888A CN201480083528.8A CN201480083528A CN107124888A CN 107124888 A CN107124888 A CN 107124888A CN 201480083528 A CN201480083528 A CN 201480083528A CN 107124888 A CN107124888 A CN 107124888A
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nucleic acid
sequence
chain
joint component
acid fragment
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CN107124888B (zh
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江媛
赵霞
李巧玲
刘生茂
王博
陈利
章文蔚
蒋慧
拉多杰·德马纳克
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Shenzhen Hua made Dazhi Technology Co. Ltd.
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BGI Shenzhen Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • Molecular Biology (AREA)
  • Biochemistry (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

本发明提供了一种鼓泡状接头元件和使用其构建测序文库的方法。所述接头元件是由一条核酸长链A和一条核酸短链B形成的两端序列互补、中间序列不互补而呈鼓泡状的杂交体。

Description

PCT国内申请，说明书已公开。

Claims (10)

  1. PCT国内申请，权利要求书已公开。
CN201480083528.8A 2014-11-21 2014-11-21 鼓泡状接头元件和使用其构建测序文库的方法 Active CN107124888B (zh)

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PCT/CN2014/091952 WO2016078095A1 (zh) 2014-11-21 2014-11-21 鼓泡状接头元件和使用其构建测序文库的方法

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CN111139533A (zh) * 2019-09-27 2020-05-12 上海英基生物科技有限公司 稳定性增加的测序文库接头
CN112795620A (zh) * 2019-11-13 2021-05-14 深圳华大基因股份有限公司 双链核酸环化方法、甲基化测序文库构建方法和试剂盒
CN113249796A (zh) * 2018-06-20 2021-08-13 深圳海普洛斯医学检验实验室 对dna片段进行标记的单分子标签

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AU2014406026B2 (en) * 2014-09-12 2018-08-23 Mgi Tech Co., Ltd. Isolated oligonucleotide and use thereof in nucleic acid sequencing
WO2018090373A1 (zh) * 2016-11-21 2018-05-24 深圳华大智造科技有限公司 一种dna末端修复与加a的方法
CN106497920A (zh) * 2016-11-21 2017-03-15 深圳华大基因研究院 一种用于非小细胞肺癌基因突变检测的文库构建方法及试剂盒
WO2019024341A1 (zh) * 2017-11-27 2019-02-07 深圳华大生命科学研究院 一种体液游离dna的文库构建方法及其应用
CN111910258B (zh) * 2020-08-19 2021-06-15 纳昂达(南京)生物科技有限公司 双端文库标签组合物及其在mgi测序平台中的应用
CN116670340A (zh) * 2020-12-29 2023-08-29 深圳华大智造科技股份有限公司 一种环化文库的快速构建方法及成环接头
EP4388091A2 (en) 2021-08-20 2024-06-26 Guardant Health, Inc. Methods for simultaneous molecular and sample barcoding
WO2023150633A2 (en) 2022-02-02 2023-08-10 Guardant Health, Inc. Multifunctional primers for paired sequencing reads
WO2024098178A1 (zh) * 2022-11-07 2024-05-16 深圳华大智造科技股份有限公司 制备dna纳米球的反应体系及其应用

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US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113249796A (zh) * 2018-06-20 2021-08-13 深圳海普洛斯医学检验实验室 对dna片段进行标记的单分子标签
CN111139533A (zh) * 2019-09-27 2020-05-12 上海英基生物科技有限公司 稳定性增加的测序文库接头
CN111139533B (zh) * 2019-09-27 2021-11-09 上海英基生物科技有限公司 稳定性增加的测序文库接头
CN112795620A (zh) * 2019-11-13 2021-05-14 深圳华大基因股份有限公司 双链核酸环化方法、甲基化测序文库构建方法和试剂盒

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US20210164042A1 (en) 2021-06-03
WO2016078095A1 (zh) 2016-05-26
US11827933B2 (en) 2023-11-28
CN107124888B (zh) 2021-08-06
US20180030532A1 (en) 2018-02-01
US10954559B2 (en) 2021-03-23

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