CN1071200A - The preparation method of quick bios for livestock and fowl - Google Patents
The preparation method of quick bios for livestock and fowl Download PDFInfo
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- CN1071200A CN1071200A CN91109504A CN91109504A CN1071200A CN 1071200 A CN1071200 A CN 1071200A CN 91109504 A CN91109504 A CN 91109504A CN 91109504 A CN91109504 A CN 91109504A CN 1071200 A CN1071200 A CN 1071200A
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- livestock
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Abstract
A kind of preparation method of preparation 906 quick bios for livestock and fowl is characterized in that utilizing the potato nutrient agar to connect gibberella, makes the production bacterial classification, and then enlarged culturing, makes rough Plant hormones regulators,gibberellins; Add 55～65% dilute hydrochloric acid again, left standstill after stirring 4～6 hours, reach sterilization fully, wash again at last, extrude liquid, promptly get the paste quick bios for livestock and fowl with pressing machine.906 quick bios for livestock and fowl of the inventive method preparation can be widely used in various livestock and poultry animals, additive of the prior art relatively, can promote the growth and the regeneration of animal body inner cell, and method for breeding is simple, fattening time is short, increase soon, also can improve disease resistance of animals.
Description
The invention belongs to a kind of preparation method of animal growth promoter, particularly a kind of growth and regeneration that promotes zooblast, the preparation method of 906 quick bios for livestock and fowl of reinforcement animal body intracellular metabolic effect.
Existing animal growth promoter, majority all is to select for use multiple nutrients materials such as mineral trace element, multivitamin, amino acid simply formulated, only to play the effect of fodder additives, what also have adds antibiotic or medicament again in above-mentioned prescription, so that it has the effect of preventing and curing diseases, but effect is unsatisfactory.
The object of the invention is to provide a kind of preparation method who utilizes Plant hormones regulators,gibberellins to prepare 906 quick bios for livestock and fowl.In prior art, Plant hormones regulators,gibberellins ethyl acetate refine, material that refine comes out or Plant hormones regulators,gibberellins, its main application is to make plant growth agent.According to method provided by the invention, can prepare growth of promotion zooblast and regeneration, strengthen the animal body intracellular metabolic, increase purpose 906 quick bios for livestock and fowl that increase weight thereby reach animal.
The present invention is achieved in that and at first utilizes the potato nutrient agar to connect gibberella, under 25 ℃～27 ℃ conditions, cultivated 5～7 days, make the production bacterial classification, and then enlarged culturing, both added compound with nutrient solution, going out connects gibberella on the nutrient agar behind the assorted bacterium, cultivates 7～10 days under 25 ℃～27 ℃ conditions, promptly makes rough Plant hormones regulators,gibberellins; Then rough Plant hormones regulators,gibberellins is added 55～65% dilute hydrochloric acid, left standstill after stirring 4～6 hours, reach sterilization fully, add warm water at last again, stir, extrude liquid, promptly get the paste quick bios for livestock and fowl with pressing machine.
Embodiment:
1, potato nutrient agar and the making of cultivating bacterial classification:
(1), potato decortication is added water (ratio that adds 100 ml waters in 20 gram potatoes adds), be heated to 70～90 ℃ then, after temperature was soaked about 1 hour, filter and remove residue was made potato and is soaked juice;
(2), potato is soaked juice adds 3% glucose (or 3% white sugar) and 3% agar, after the heating for dissolving, in the injecting tube,, take out the back and cools naturally with 1.3 kilograms autoclavings 30 minutes, the solid bacteria substratum;
(3), under aseptic condition, gibberella is received on the face of solid bacteria substratum with connecing collarium;
(4), the test tube that connects bacterium is put into thermostat container, under 25 ℃～27 ℃ temperature, cultivated 5～7 days, promptly make the production bacterial classification.
2, enlarged culturing
(1) making contains sugar, potassium, amine, the nutrient solution of iron, the multiple composition of zinc;
White sugar with 2%, 0.6% potassium primary phosphate, 0.25% sulfuric acid amine, 0.005% ferrous sulfate and 0.005% zinc sulfate add in 1000 ml waters, and it is standby to stir;
Or 2% white sugar (or glucose), 0.3% Repone K, 0.3% SODIUM PHOSPHATE, MONOBASIC, 0.25% sulfuric acid amine, 0.005% ferrous sulfate, 0.005 zinc sulfate added in 1000 ml waters, it is standby to stir;
In above-mentioned nutrient solution, can also add two again by 5 gram Sodium orthomolybdates, 5 gram boric acid and the formulated trace elements of 1000 ml waters.
(2), make compound
Stir 20% jowar chaff, 20% Semen Maydis powder (or 18% Semen Maydis powder, 2% bean powder), 60% wheat bran standby;
(3), the ratio adding nutrient solution that above-mentioned compound is added in 30% stirs, make the production substratum, should produce substratum and put into container, with go out assorted bacterium 30 minutes of 1.3 kilograms high pressure, go out and cool naturally behind the assorted bacterium, connect the gibberella on the nutrient agar in sterilisable chamber, 25 ℃～27 ℃ constant temperature indoor cultivation 7～10 days, growth was finished, make rough Plant hormones regulators,gibberellins
The making of 3,906 growth hormone
(1), sterilization, supermature rough Plant hormones regulators,gibberellins is sterilized with dilute hydrochloric acid, and said dilute hydrochloric acid is 55%～65% hydrochloric acid, 45%～35% water, its optimum proportion is 60% hydrochloric acid, 40% water behind the dilute hydrochloric acid of stating in the adding in rough Plant hormones regulators,gibberellins, stirs, left standstill 4～6 hours, and reached sterilization fully.
(2) washing, with the warm water of 906 growth hormone after the sterilization by 50 ℃ of 1: 1 ratio addings, stir, extrude liquid with pressing machine then, promptly get two kinds of finished products, a kind of is aqua, and a kind of is paste, wherein paste is 906 quick bios for livestock and fowl of the inventive method preparation, and aqua can be used as plant growth agent.
906 quick bios for livestock and fowl of the inventive method preparation can be widely used in various livestock and poultry animals, as pig, ox, sheep, chicken, duck, goose etc. during use, add with 2% of dry feed consumption and to get final product, additive of the prior art relatively can promote the growth and the regeneration of animal body inner cell, uses as additive, then consumption is few, relative price is low, and method for breeding is simple, and fattening time is short, increase soon, also can improve disease resistance of animals.
Claims (6)
1, a kind of preparation method of quick bios for livestock and fowl, it is characterized in that, utilizing the potato nutrient agar to connect gibberella cultivates, under 25 ℃～27 ℃ conditions, cultivated 5～7 days, make the production bacterial classification, and then enlarged culturing, both added compound with nutrient solution, the gibberella that connects on the nutrient agar behind the assorted bacterium of going out is cultivated again, cultivates 7～10 days under 25 ℃～27 ℃ conditions, add 55～65% dilute hydrochloric acid then, left standstill after stirring 4～6 hours, and reached sterilization fully, add warm water at last again and stir, extrude liquid with pressing machine, promptly get the paste quick bios for livestock and fowl.
2, according to the preparation method of the said quick bios for livestock and fowl of claim 1, it is characterized in that, contain 2% white sugar in the said nutrient solution, 0.6% potassium primary phosphate, 0.25% sulfuric acid amine, 0.005% ferrous sulfate and 0.005% zinc sulfate.
3, according to the preparation method of the said quick bios for livestock and fowl of claim 1, it is characterized in that, contain 0.2% white sugar in the said nutrient solution, 0.3% greening potassium, 0.3% SODIUM PHOSPHATE, MONOBASIC, 0.25% sulfuric acid amine, 0.005% ferrous sulfate, 0.005% zinc sulfate.
4, according to the preparation method of claim 2 or 3 said quick bios for livestock and fowl, it is characterized in that, can also add two in the said nutrient solution by 5 gram Sodium orthomolybdates, the formulated trace element of 5 gram boric acid.
According to the preparation method of the said quick bios for livestock and fowl of claim 1, it is characterized in that 5, said compound is 20% jowar chaff, 20% Semen Maydis powder, 60% wheat bran or 20% jowar chaff, 18% Semen Maydis powder, 2% bean powder, 60% wheat bran.
According to the preparation method of the said quick bios for livestock and fowl of claim 1, it is characterized in that 6, said dilute hydrochloric acid optimum proportion is 60% hydrochloric acid, 40% water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN91109504A CN1071200A (en) | 1991-09-26 | 1991-09-26 | The preparation method of quick bios for livestock and fowl |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN91109504A CN1071200A (en) | 1991-09-26 | 1991-09-26 | The preparation method of quick bios for livestock and fowl |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1071200A true CN1071200A (en) | 1993-04-21 |
Family
ID=4909871
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN91109504A Pending CN1071200A (en) | 1991-09-26 | 1991-09-26 | The preparation method of quick bios for livestock and fowl |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1071200A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006111542A1 (en) * | 2005-04-21 | 2006-10-26 | Desol Bv | Method for increasing the speed of recovery and/or the speed of wound healing of injured animals |
| US8084440B2 (en) | 2005-04-21 | 2011-12-27 | Desol B.V. | Method for improving the fertility of animals |
-
1991
- 1991-09-26 CN CN91109504A patent/CN1071200A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006111542A1 (en) * | 2005-04-21 | 2006-10-26 | Desol Bv | Method for increasing the speed of recovery and/or the speed of wound healing of injured animals |
| EA013523B1 (en) * | 2005-04-21 | 2010-06-30 | Десол Бв | Method for increasing the speed of recovery of wounded animals and/or the speed of wound healing of injured animals and animal food therefor |
| US8084440B2 (en) | 2005-04-21 | 2011-12-27 | Desol B.V. | Method for improving the fertility of animals |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |