CN107114606A - A kind of method for improving artemia ovum hatching synchronism - Google Patents
A kind of method for improving artemia ovum hatching synchronism Download PDFInfo
- Publication number
- CN107114606A CN107114606A CN201710314776.4A CN201710314776A CN107114606A CN 107114606 A CN107114606 A CN 107114606A CN 201710314776 A CN201710314776 A CN 201710314776A CN 107114606 A CN107114606 A CN 107114606A
- Authority
- CN
- China
- Prior art keywords
- artemia
- hatching
- synchronism
- nauplius
- improving
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000012447 hatching Effects 0.000 title claims abstract description 36
- 241001247197 Cephalocarida Species 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 18
- 102000002322 Egg Proteins Human genes 0.000 title claims abstract description 17
- 108010000912 Egg Proteins Proteins 0.000 title claims abstract description 17
- 210000004681 ovum Anatomy 0.000 title claims abstract description 17
- 241000133262 Nauplius Species 0.000 claims abstract description 16
- 238000011534 incubation Methods 0.000 claims abstract description 13
- 208000031513 cyst Diseases 0.000 claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 239000008363 phosphate buffer Substances 0.000 claims description 3
- 229910052736 halogen Inorganic materials 0.000 claims description 2
- 150000002367 halogens Chemical class 0.000 claims description 2
- 241000607598 Vibrio Species 0.000 abstract description 9
- 206010047400 Vibrio infections Diseases 0.000 abstract description 9
- 235000016709 nutrition Nutrition 0.000 abstract description 6
- 239000004615 ingredient Substances 0.000 abstract description 5
- 239000013535 sea water Substances 0.000 description 3
- 241000238582 Artemia Species 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 210000000436 anus Anatomy 0.000 description 2
- 210000002969 egg yolk Anatomy 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 210000001136 chorion Anatomy 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 150000003016 phosphoric acids Chemical class 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biomedical Technology (AREA)
- Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of method for improving artemia ovum hatching synchronism, after artemia cysts is pressed into normal method artificial incubation 8 12 hours, the artemia ovum hatching liquid extract prepared is added in the ratio of 50 150 mg/litres, then proceed to hatching no longer to dramatically increase to incubation rate, you can collect nauplius.This method is easily operated, pollution-free using natural extract.The synchronism of artemia ovum hatching is effectively increased, the nutritional ingredient of nauplius is at utmost remained, the utilization rate of artemia cysts is improved, while reducing the risk that the young carries vibrios.
Description
Technical field
The present invention relates to a kind of hatching method of artemia cysts, and in particular to a kind of side of raising artemia ovum hatching synchronism
Method.
Background technology
Artemia cysts is commonly used in aquaculture industry, and incubation rate is always concerned focus.But nauplius
The synchronism of hatching is never by enough attention.Nauplius is from hatching to feeding in the time of experience, and nauplius will
Sustained life by consuming the yolk bag of itself, so the nutritional ingredient loss of this time longer nauplius is bigger.Generally
In the case of, the nauplius by hatching in some hours out, starts swimming life from chorion, and general body length is no more than 500
Micron.Now without mouth, enteron aisle and anus, the artemia larvae in this stage is referred to as " the I phase young ".Paedomorphosis shape quickly
Into mouth, enteron aisle, anus, body length is more than 700 microns, at this moment referred to as " the II phase young ".Due to motion power consumption, than " I phase children
Body ", the nutritional ingredient that " the II phase young " is accumulated significantly is consumed, and moisture is increased considerably, and the artemia of this state is not only
It can not be called " high-quality ", or even it is difficult therefrom to be absorbed to effective nutriment afterwards that aquatic livestock is feeding.Many seedlings also make
Exceed the artemia by more than 50 hours as food organisms with brooding time.According to statistics, the good young of hatching synchronism is than tradition
The young obtained by technology can at least provide 20% nutriment more.Except more nutriments can be provided, come for artemia cysts
Say that the risk that can evade carrying vibrios in hatching process is equally very crucial.After artemia ovum hatching, if artemia larvae is carried
The cause of diseases such as vibrios, can cause huge puzzlement to seedling stage.Finished product artemia cysts does not carry vibrios in itself under normal circumstances, and vibrios is main
Be to bring worm's ovum into by media such as air in hatching process, it is then quick in the suitable hatching water body of nutritious, condition
Breeding is got up, once and the II phases are arrived in artemia larvae development, mouth and alimentary canal are formd, vibrios then can enter worm from alimentary canal
Body, now even thoroughly sterilization also is difficult to remove vibrios.
The content of the invention
It is an object of the invention to provide a kind of method for improving artemia ovum hatching synchronism, the profit for improving artemia cysts
Reduce the risk that nauplius carries vibrios simultaneously with rate.
To achieve the above object, the technical measures that the present invention takes are as follows：
After artemia cysts was by normal method artificial incubation 8-12 hours, the halogen prepared is weighed in the ratio of 50-150 mg/litres
Egg hatch liquid extract, extract is first dissolved in phosphate buffer, is then added in hatching system, and 25 DEG C are continued to be incubated
No longer dramatically increased with up to incubation rate within 10 hours, you can collect nauplius.
The preparation method of artemia ovum hatching liquid extract is in the above method：
1. after artemia cysts is fully cleaned, be hydrated, ovum is scattered in triangular flask in the ratio of every gram of dry 100 milliliters of artificial seawater of ovum
In, shaking table 100rpm/min vibrations, 25 DEG C of temperature；
2. after hatching 28 hours, 0.8% polyethylene glycol, ultrasonication 20 seconds, power density 0.5w/cm are added in artemia hatching solution2,
Centrifuge, collect artemia hatching solution.Precipitation is redissolved in 100 milliliters of artificial seawaters, stirred 5 minutes, is centrifuged, will twice from
Supernatant merges；
3. artemia hatching solution is centrifuged, rotating speed 500g, 5 minutes, it was 55% that supernatant, which adds ammonium sulfate to saturation degree, by precipitation phosphoric acid
Salt buffer dissolves again, sephadex SephadexG-25 chromatography desalinations, freezes.
The present invention has the following advantages：
The synchronism of artemia ovum hatching is effectively increased, time of the artemia nauplii from hatching to feeding is shortened, reduces
Nauplius nutrition and the consumption of energy, at utmost remain the nutritional ingredient of nauplius, improve the utilization of artemia cysts
Rate, reduces the risk that the young carries vibrios.This method is easily operated, pollution-free using natural extract.
Embodiment
The inventive method is described in further detail with reference to specific embodiment.
Embodiment
The artemia cysts of Tianjin is originated in, the requirement of incubation rate, the soaking hydration of 400 ovum of every group of selection are tested according to several methods
Afterwards, 28 DEG C are incubated, seawater salinity 29 ‰, Aerated stirring starts artificial incubation.After 10 hours, weighed in the ratio of 50 mg/litres
Artemia ovum hatching liquid extract, extract is first dissolved in phosphate buffer, is then added in hatching system, 25 DEG C of insulations.
In hatching, 20 hours started to 36 hours, and the nauplius hatched is collected per hour, incubation rate is calculated using several methods, as a result
Such as table 1.The obvious time span of control group incubation rate amplification is 8 hours, experimental group corresponding time it can be seen from the data of table 1
Span is 4 hours, and period, two groups of incubation rate amplification were close, but required time significant difference；Experimental group brooding time is compared with control group
Shift to an earlier date.Because temperature height can accelerate hatching liquid extract degraded, this experiment incubation temperature elects relatively low 25 DEG C as, makes hatching
Time increased relatively.After nauplius hatching, quick swimming is not stopped in the seawater, required big energy and nutriment are all
Supplied by itself yolk bag.Brooding time span is big, and the nauplius consumption first hatched is more, and nutritive value loss is big, reduces
Bait utilization ratio.Hatch synchronism good, can in time be fed in the short period after hatching, nutritional ingredient contained by nauplius
Retain many, so it is significant for actual production to improve hatching synchronism.The present invention is same for improving artemia ovum hatching
Step property effect is notable.
Claims (1)
1. a kind of method for improving artemia ovum hatching synchronism, it is characterized in that step is as follows：
After artemia cysts was by normal method artificial incubation 8-12 hours, the halogen prepared is weighed in the ratio of 50-150 mg/litres
Egg hatch liquid extract, extract is first dissolved in phosphate buffer, is then added in hatching system, and 25 DEG C are continued to be incubated
No longer dramatically increased with up to incubation rate within 10 hours, you can collect nauplius.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710314776.4A CN107114606A (en) | 2017-05-07 | 2017-05-07 | A kind of method for improving artemia ovum hatching synchronism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710314776.4A CN107114606A (en) | 2017-05-07 | 2017-05-07 | A kind of method for improving artemia ovum hatching synchronism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107114606A true CN107114606A (en) | 2017-09-01 |
Family
ID=59727681
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710314776.4A Pending CN107114606A (en) | 2017-05-07 | 2017-05-07 | A kind of method for improving artemia ovum hatching synchronism |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107114606A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107711640A (en) * | 2017-11-02 | 2018-02-23 | 广西沙田仙人滩农业投资有限公司 | A kind of method for fine finishing of artemia cysts |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699561A (en) * | 2005-05-05 | 2005-11-23 | 中国海洋大学 | Process for preparing artemia hatching enzyme by using artemia hatching liquid |
| CN101032236A (en) * | 2007-03-23 | 2007-09-12 | 浙江省淡水水产研究所 | Hatching method of the ovum of Branchinella kugennumaensis |
| CN101843237A (en) * | 2009-03-26 | 2010-09-29 | 刘学刚 | New method for ensuring quality of artemia eggs in salt lake through physiological action |
| RU2011127289A (en) * | 2011-07-01 | 2013-01-10 | Федеральное государственное образовательное учреждение высшего профессионального образования "Кемеровский государственный сельскохозяйственный институт" | METHOD FOR PREPARING ARTINY SALIN EGGS FOR INCUBATION |
-
2017
- 2017-05-07 CN CN201710314776.4A patent/CN107114606A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699561A (en) * | 2005-05-05 | 2005-11-23 | 中国海洋大学 | Process for preparing artemia hatching enzyme by using artemia hatching liquid |
| CN101032236A (en) * | 2007-03-23 | 2007-09-12 | 浙江省淡水水产研究所 | Hatching method of the ovum of Branchinella kugennumaensis |
| CN101843237A (en) * | 2009-03-26 | 2010-09-29 | 刘学刚 | New method for ensuring quality of artemia eggs in salt lake through physiological action |
| RU2011127289A (en) * | 2011-07-01 | 2013-01-10 | Федеральное государственное образовательное учреждение высшего профессионального образования "Кемеровский государственный сельскохозяйственный институт" | METHOD FOR PREPARING ARTINY SALIN EGGS FOR INCUBATION |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107711640A (en) * | 2017-11-02 | 2018-02-23 | 广西沙田仙人滩农业投资有限公司 | A kind of method for fine finishing of artemia cysts |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170901 |
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