CN107114224A - A kind of water floatation seedling method of Sedum alfredii Hance - Google Patents
A kind of water floatation seedling method of Sedum alfredii Hance Download PDFInfo
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- CN107114224A CN107114224A CN201710335502.3A CN201710335502A CN107114224A CN 107114224 A CN107114224 A CN 107114224A CN 201710335502 A CN201710335502 A CN 201710335502A CN 107114224 A CN107114224 A CN 107114224A
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- cuttings
- water
- seedling method
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- promoting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The invention discloses a kind of water floatation seedling method of Sedum alfredii Hance, comprise the following steps：(1) raw, the Sedum alfredii Hance overground part branch of no disease and pests harm then is gathered, cuttings is cut into, being placed at shady and cool ventilation makes its wound healing；(2) cuttage is on cystosepiment with holes after the cuttings the latter half after wound healing is soaked in growth-promoting liquid, the cystosepiment is floated in clear water, make the latter half of cuttings in clear water contact, top half and air contact, the growth-promoting liquid is auxin solution or plant growth-promoting bacteria bacterium solution；(clear water is regularly replaced during 3 nursery, after after root system length to 5cm, you can for transplanting.The inventive method method is not subject to seasonal restrictions, highly intensive, and unit area is emerged many, is emerged fast, industrialization prospect is good, realizes the extensive nursery of batch production, quickly can provide a large amount of seedlings for heavy metal-polluted soil reparation in short term；Preferably, seedling is more healthy and strong, no weeds, and no soil-borne disease insect pest, transplanting survival rate is higher for seedling root system development；Seedbed is saved, manpower and materials are saved.
Description
Technical field
The invention belongs to plant propagation and nursery field, it is related to a kind of water floatation seedling method of Sedum alfredii Hance.
Background technology
Cadmium Pollution in Soils is increasingly serious in recent years, and the cadmium in soil enters human body by food chain, is detrimental to health.Such as
What is administered and mitigates the cadmium pollution in soil, as current urgent problem.Phytoremediation is to utilize heavy metal hyperaccumulative
Or enriching plant administer a kind of of heavy metal pollution of soil compare the mode for having environmentally friendly future, with it is low-cost, without secondary dirt
Contaminate, economize the land resource, environment-friendly, sustainable development the advantages of.Sedum alfredii Hance (Sedum alfredii Hance) be
A kind of zinc, Cd hyperaccumulator, the lead enriching plant found first on the ancient Pb-Zn deposits in Quzhou City of Zhejiang Province of China one, closely
The phytoremediation of heavy-metal contaminated soil is widely used in over year.However, large-scale phytoremediation needs a large amount of Sedum alfredii Hance kinds
Seedling, as being a kind of wild plant, the artificial propagation techniques of Sedum alfredii Hance are researcher concern all the time.East
Southern red-spotted stonecrop is a kind of perennial angiosperm of Crassulaceae Sedum, and vitality is strong, both can be with generative propagation, again can be with asexual numerous
Grow, be distributed widely in China Central China, south China and East China.
Existing patent CN101057542A discloses a kind of sexual reproduction method of Sedum alfredii Hance, and this method is related to seed and adopted
The technologies such as receipts, Seed storage, germination and seedling management.Although the problem of this method solve certain, such as save
Sedum alfredii Hance germ plasm resource, but as a kind of plant based on vegetative propagation, the sexual reproduction technique of Sedum alfredii Hance has
Seed hardly possible collection, seedling raise period is long, the shortcomings of offspring's heavy metal accumulation ability is degenerated.Meanwhile, existing patent CN1973617A
A kind of asexual reproduction method of heavy metal hyperaccumulative plant Sedum alfredii Hance is disclosed with CN101444185A, this method is related to the southeast
The technologies such as red-spotted stonecrop blade callus induction differentiation technique, stem-tip tissue fast breeding technique and stem section cuttage technique.Though these methods
So a large amount of seedlings can be obtained, but the asexual reproduction method based on plant tissue culture technique have cost height, seedling raise period length,
The low shortcoming of seedling replanting survival rate；There is seedling cost with the stem section cuttage technique of seedling medium or organic substrate or nutrient solution
High, transport inconvenience, the shortcomings of take time and effort, it is difficult to the need for meeting large-scale industrialized nursery.
The content of the invention
Need rapidly to obtain a large amount of Sedum alfredii Hance germchits in short term to solve the large-scale phytoremediation of Current Domestic
Current demand, the present invention provides a kind of water floatation seedling method of Sedum alfredii Hance.
A kind of water floatation seedling method of Sedum alfredii Hance, comprises the following steps：
(1) raw, the Sedum alfredii Hance overground part branch of no disease and pests harm then is gathered, cuttings is cut into, placed at shady and cool ventilation
Make its wound healing；
(2) cuttage is on cystosepiment with holes after the cuttings the latter half after wound healing is soaked in growth-promoting liquid, institute
State cystosepiment to float in clear water, make the latter half of cuttings in clear water contact, top half and air contact, the growth-promoting
Liquid is auxin solution or plant growth-promoting bacteria bacterium solution；
(clear water is regularly replaced during 3 nursery, after after root system length to 5cm, you can for transplanting.
The inventive method method is not subject to seasonal restrictions, highly intensive, and unit area is emerged many, is emerged fast, before industrialization
Jing Hao, realizes the extensive nursery of batch production, quickly can provide a large amount of seedlings for heavy metal-polluted soil reparation in short term；Seedling root system
Development is preferable, and seedling is more healthy and strong, no weeds, and no soil-borne disease insect pest, transplanting survival rate is higher；Seedbed is saved, manpower thing is saved
Power.
Preferably, the length of cuttings described in step (1) is that the standing time at 5~6cm, shady and cool ventilation is small for 20~25
When.It is further preferred that the standing time at shady and cool ventilation is 24 hours.
Preferably, the auxin in the auxin solution is heteroauxin.The heteroauxin is that one kind can promote to plant
The material that thing roots, the generally existing in plant.
Preferably, the concentration of heteroauxin is 0.5~2 μM in the auxin solution；More preferably 0.8~1.2 μ
M；Most preferably 1 μM.
The preparation method of auxin solution (by taking 1 μM of concentration as an example)：1.7519g auxin is weighed, 1L clear water is dissolved in, plus it is few
Amount sodium hydroxide helps it to dissolve, and this auxin solution is diluted into 10,000 times, and now solution concentration is 1 μM, standby.
Preferably, the plant growth-promoting bacteria in the plant growth-promoting bacteria bacterium solution is the fluorescence that preserving number is CGMCC No.12173
Pseudomonas alba (Pseudomonas fluorescens) Sasm05.
The plant growth-promoting bacteria that the present invention is used is that Sasm05 is that isolated one kind out of Sedum alfredii Hance body can be secreted
Auxin and the beneficial bacteria for promoting plant growth, belong to Pseudomonas fluorescence (Pseudomonas fluorescens),
Preservation is to China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number：CGMCC No.12173, and
Disclosed in Publication No. CN 105950502A Patent Application Publication.
It is further preferred that the concentration of plant growth-promoting bacteria is entered using OD600 as 0.5~1.5 in the plant growth-promoting bacteria bacterium solution
Row is prepared；More preferably OD600=1.0.
The preparation method of plant growth-promoting bacteria bacterium solution (by taking OD600=1.0 as an example)：1mL is drawn on superclean bench to preserve
Strain injection 50mL LB fluid nutrient mediums (contain per 1L：Sodium chloride 10g, dusty yeast 5g, peptone 10g, pH are 7.2) sealing
After 150rpm, 35 DEG C of shaking table culture 24h, bacterium solution is precipitated for 15 minutes in 5000rpm centrifugations, with clear water dissolving precipitation simultaneously
It is diluted to OD600=1.0 standby.
Preferably, soak time of the cuttings in growth-promoting liquid is 3~5 hours；It is further preferred that soaking 4 hours.
Preferably, when being soaked in growth-promoting liquid, every 35~45 a bundles are soaked；It is further preferred that every 40
A bundle is soaked.
Preferably, cuttage distance between rows and hills of the cuttings on cystosepiment is (3~5) × (3~5) cm；More preferably 4
×4cm。
The cystosepiment uses 30 × 20 × 0.5cm cystosepiments with holes, and the cystosepiment floats on the plastics for filling clear water
In square frame, per 40 cuttings of frame.
Preferably, a clear water is changed within every 3~5 days during nursery；It is further preferred that every 4 days during nursery change one
Secondary clear water.
Treat that root system is grown to after 5cm (about 14 days), seedling is removed into water body, after being placed 24 hours at shady and cool ventilation
Transplant.
It is further preferred that Sedum alfredii Hance branch is cut into 5-6cm cuttings by the inventive method, after cuttings is placed one day
In the inner immersion of growth-promoting liquid (IAA or Sasm05) 4 hours, then soaking growth-promoting liquid and do not soaked the cuttings point of growth-promoting liquid
Other cuttage is on cystosepiment, and cystosepiment is swum on clear water, changes within four days a clear water, root system grows to 5cm or so after 14 days
Transplant；Most preferably, the growth-promoting liquid is Sasm05.
Compared with the conventional method, the present invention has the advantages that：
1) nursery of the present invention is not subject to seasonal restrictions, highly intensive, and unit area is emerged many, is emerged fast, industrialization prospect
It is good, the extensive nursery of batch production is realized, quickly a large amount of seedlings can be provided for heavy metal-polluted soil reparation in short term；
2) present invention breeds Sedum alfredii Hance germchit using water floatation seedling mode, and root system is not limited by soil or seedling medium
System, root system development is good, and root/shoot ratio is big, and seedling is healthy and strong, no weeds, no soil-borne disease insect pest, and transplanting survival rate is high；
3) present invention breeds Sedum alfredii Hance germchit using water floatation seedling mode, saves seedbed, lifting, fortune seedling, transplanting
Simplify, save manpower and materials, the method is easy to apply among actual production.
Brief description of the drawings
Fig. 1 is Sedum alfredii Hance water floatation seedling flow chart of the present invention.
The Sedum alfredii Hance root growth of embodiment 1~3 and development comparison diagram when Fig. 2 is water planting 14 days.
Embodiment
With reference to specific embodiment, the invention will be further described：
Embodiment one, water floatation seedling
1) raw, the Sedum alfredii Hance overground part branch of no disease and pests harm then is gathered, 5-6cm cuttings is cut into, in shady and cool ventilation
Place is placed 24 hours, makes its wound healing.
2) cuttings after placement is pressed the cuttage of 4 × 4cm distances between rows and hills on 30 × 20 × 0.5cm cystosepiments with holes, foam
Plate is swum in the plastics square frame that 30 × 20 × 10cm fills clear water, cuttings the latter half is contacted with clear water, top half with
Air contact, per 40 cuttings of frame.
3) change a clear water within four days during nursery, treat that root system is grown to after 5cm (about 14 days), seedling is removed into water body, in
It can be transplanted after being placed 24 hours at shady and cool ventilation.
This method is not subject to seasonal restrictions, highly intensive, and unit area is emerged many, is emerged fast, industrialization prospect is good, realizes
The extensive nursery of batch production, quickly can provide a large amount of seedlings for heavy metal-polluted soil reparation in short term；Seedling root system development preferably, is planted
Seedling is more healthy and strong, no weeds, and no soil-borne disease insect pest, transplanting survival rate is higher；Seedbed is saved, manpower and materials are saved.
Embodiment two, auxin auxiliary water floatation seedling
1) raw, the Sedum alfredii Hance overground part branch of no disease and pests harm then is gathered, 5-6cm cuttings is cut into, in shady and cool ventilation
Place is placed 24 hours, makes its wound healing.
2) 40 a bundles of cuttings after placement are tied, cuttings the latter half is soaked 4 in 1 μM of auxin solution small
When.
3) cuttings after immersion is pressed the cuttage of 4 × 4cm distances between rows and hills on 30 × 20 × 0.5cm cystosepiments with holes, foam
Plate is swum in the plastics square frame that 30 × 20 × 10cm fills clear water, cuttings the latter half is contacted with clear water, top half with
Air contact, per 40 cuttings of frame.
4) change a clear water within four days during nursery, treat that root system is grown to after 5cm (about 14 days), seedling is removed into water body, in
It can be transplanted after being placed 24 hours at shady and cool ventilation.
The effect for promoting plant establishment germination because auxin has, growing, obtains compare embodiment in this way
One more preferable nursery effect, more preferably, seedling is more healthy and strong, and transplanting survival rate is higher for seedling root system development.
Embodiment three, plant growth-promoting bacteria auxiliary water floatation seedling
1) raw, the Sedum alfredii Hance overground part branch of no disease and pests harm then is gathered, 5-6cm cuttings is cut into, in shady and cool ventilation
Place is placed 24 hours, makes its wound healing.
2) 40 a bundles of cuttings after placement are tied, makes cuttings the latter half in OD600=1.0 plant growth-promoting bacteria bacterium
The inner immersion of liquid (Sasm05) 4 hours.
3) cuttings after immersion is pressed the cuttage of 4 × 4cm distances between rows and hills on 30 × 20 × 0.5cm cystosepiments with holes, foam
Plate is swum in the plastics square frame that 30 × 20 × 10cm fills clear water, cuttings the latter half is contacted with clear water, top half with
Air contact, per 40 cuttings of frame.
4) change a clear water within four days during nursery, treat that root system is grown to after 5cm (about 14 days), seedling is removed into water body, in
It can be transplanted after being placed 24 hours at shady and cool ventilation.
Because plant growth-promoting bacteria Sasm05 can secrete auxin, research shows：The processing of Sasm05 bacterium solutions is to Sedum alfredii Hance
Growth-promoting effect better than auxin processing, obtain in this way than the more preferable nursery effect of embodiment two, seedling root system development
In three embodiments preferably, seedling is most healthy and strong, transplanting survival rate highest.
The specific implementation case of patent of the present invention is the foregoing is only, but the technical characteristic of patent of the present invention is not limited to
This, any those skilled in the relevant art are in the field of the invention, and the change or modification made all are covered in the special of the present invention
Among sharp scope.
Claims (10)
1. a kind of water floatation seedling method of Sedum alfredii Hance, it is characterised in that comprise the following steps：
(1) raw, the Sedum alfredii Hance overground part branch of no disease and pests harm then is gathered, cuttings is cut into, being placed at shady and cool ventilation makes it
Wound healing；
(2) cuttage is on cystosepiment with holes after the cuttings the latter half after wound healing is soaked in growth-promoting liquid, the bubble
Foam plate is floated in clear water, the latter half of cuttings is contacted with clear water, top half and air contact, and the growth-promoting liquid is made a living
Long element solution or plant growth-promoting bacteria bacterium solution；
(3) clear water is regularly replaced during nursery, after after root system length to 5cm, you can for transplanting.
2. water floatation seedling method according to claim 1, it is characterised in that the length of cuttings described in step (1) is 5
Standing time at~6cm, shady and cool ventilation is 20~25 hours.
3. water floatation seedling method according to claim 1, it is characterised in that the auxin in the auxin solution is
Heteroauxin.
4. water floatation seedling method according to claim 3, it is characterised in that heteroauxin in the auxin solution
Concentration is 0.5~2 μM.
5. water floatation seedling method according to claim 1, it is characterised in that the plant in the plant growth-promoting bacteria bacterium solution
Promoting bacteria is the Pseudomonas fluorescence (Pseudomonas fluorescens) that preserving number is CGMCC No.12173
Sasm05。
6. water floatation seedling method according to claim 5, it is characterised in that plant promotees in the plant growth-promoting bacteria bacterium solution
The concentration of raw bacterium is prepared using OD600 as 0.5~1.5.
7. water floatation seedling method according to claim 1, it is characterised in that soak time of the cuttings in growth-promoting liquid be
3~5 hours.
8. water floatation seedling method according to claim 1, it is characterised in that cuttage row of the cuttings on cystosepiment
Spacing in the rows is (3~5) × (3~5) cm.
9. water floatation seedling method according to claim 1, it is characterised in that change once clear in every 3~5 days during nursery
Water.
10. water floatation seedling method according to claim 1, it is characterised in that change once clear in every 4 days during nursery
Water.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114158364A (en) * | 2020-09-09 | 2022-03-11 | 深圳文科园林股份有限公司 | Sedum alfredii cutting propagation method |
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2017
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| CN102499043A (en) * | 2011-11-21 | 2012-06-20 | 江苏省农业科学院 | Cutting water culture rooting method for jasminum sambac |
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| CN105950502A (en) * | 2016-05-17 | 2016-09-21 | 浙江大学 | Complex endophytic bacterial inoculant and application thereof in phytoremediation of heavy metal contaminated soil |
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Cited By (1)
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| CN114158364A (en) * | 2020-09-09 | 2022-03-11 | 深圳文科园林股份有限公司 | Sedum alfredii cutting propagation method |
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Application publication date: 20170901 |