CN107080796B - Traditional Chinese medicine preparation for treating osteoarthritis - Google Patents
Traditional Chinese medicine preparation for treating osteoarthritis Download PDFInfo
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine preparation for treating pain, swelling, numbness, difficulty in flexion and extension of joints, joint stiffness and malformation symptoms caused by osteoarthritis and proliferative arthritis. The traditional Chinese medicine preparation comprises the following raw material medicines in parts by weight: 8-13 parts of antler, 8-12 parts of achyranthes root, 8-12 parts of orientvine stem, 10-15 parts of Ningpo yam rhizome and 8-13 parts of fried white paeony root. The invention is based on liver and kidney deficiency and meridian obstruction, a classical meridian prescription of 'antler pill', and years of clinical experience of orthopedics specialists in affiliated hospitals of Liaoning Chinese medicine university for treating osteoarthritis, the invention is prepared by continuously adjusting the medicine dosage and adding flavor, and the summarized clinical experience formula is obtained; is an effective medicine prepared from pure traditional Chinese medicines and used for treating pain, swelling, numbness, difficulty in bending and stretching, joint stiffness and malformation caused by osteoarthritis and proliferative arthritis. Animal experiments prove that the traditional Chinese medicine composition has obvious analgesic and anti-inflammatory effects.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine preparation for treating osteoarthritis, in particular to a traditional Chinese medicine preparation for treating pain, swelling, numbness, difficulty in flexion and extension of joints, joint stiffness and malformation diseases caused by osteoarthritis and proliferative arthritis.
Background
Osteoarthritis is a common disease, mostly occurring in middle-aged years, and belongs to the category of arthralgia-bone disease in traditional Chinese medicine. Arthralgia is caused by invasion of exogenous pathogenic factors such as wind, cold and dampness, obstruction of meridians, and unsmooth circulation of qi and blood, and has the main clinical manifestations of pain, swelling, numbness, difficulty in flexion and extension of joints, even stiffness and deformity of joints. The liver governs tendons and bones, the kidney governs bones, the six seven women and the six eight men, the condition of deficiency and weakness gradually shows, "liver qi deficiency, tendon immobility", "kidney deficiency and all-body extreme", clinically, the tendon is urgent and contracture, the action is strong, or flaccidity and weakness. The excess of this disease is the syndrome of deficiency with excess of origin. Deficiency of the liver and kidney can cause malnutrition of the muscles and bones; excess syndrome, empty and deficient striae, and wind-cold-dampness obstructing the meridians and collaterals, manifested as arthralgia. Clinical verification and summary report of diagnosis and treatment scheme of 'fifteen' bone rheumatism (osteoarthritis) of the national administration of traditional Chinese medicine, 53 cases of cold-dampness collateral-blocking syndromes, 50 cases of dampness-heat collateral-blocking syndromes, 196 cases of liver and kidney deficiency syndromes and 21 cases of qi and blood deficiency syndromes in 320 patients indicate that the principal deficiency, marked excess and liver and kidney deficiency are the main pathogenesis of the disease. Clinically, the traditional Chinese medicine treatment of osteoarthritis is divided into an internal treatment method and an external treatment method, wherein the internal treatment method mainly adopts oral administration of traditional Chinese medicines, and clinically common oral traditional Chinese medicines are complex in formula, large in number of medicinal ingredients, complex in decoction method and high in medicine cost. Therefore, it is very important to further research the traditional Chinese medicine composition with few raw materials and strong pertinence.
Disclosure of Invention
Aiming at the problems, the invention provides a traditional Chinese medicine preparation for treating osteoarthritis, in particular for treating pain, swelling, numbness, difficulty in flexion and extension of joints, joint stiffness and deformity caused by osteoarthritis and proliferative arthritis. The traditional Chinese medicine preparation has the advantages of few raw materials, good curative effect, strong pertinence and obvious clinical application effect.
In order to achieve the purpose, the invention adopts the following technical scheme: the traditional Chinese medicine preparation comprises the following raw material medicines in parts by weight: 8-13 parts of antler, 8-12 parts of achyranthes root, 8-12 parts of orientvine stem, 10-15 parts of Ningpo yam rhizome and 8-13 parts of fried white paeony root.
The preparation is a clinically acceptable medicinal preparation such as pills, powder, mixture, disintegrating tablets or dispersible tablets.
The preparation method of the traditional Chinese medicine preparation comprises the following steps: the raw materials are weighed according to the weight portion, added with water for decoction, added with water according to 8 times of the total weight of the medicine, decocted for 2 times, 1.5 hours each time, filtered, combined with filtrate, concentrated, added with auxiliary materials acceptable for clinical medicine preparations and prepared into the required preparation formulation.
Analyzing the drug properties of the raw materials.
Deer horn: salty and warm, entering kidney and liver meridians; has the effects of warming kidney yang, strengthening tendons and bones, promoting blood circulation and relieving swelling.
Caulis Sinomenii: bitter, pungent and mild, entering liver and spleen meridians; has the effects of dispelling wind-damp and dredging the channels and collaterals.
Achyranthes root: bitter, sweet, sour and neutral, entering liver and kidney meridians; has the functions of nourishing liver and kidney, strengthening bones and muscles, removing blood stasis and dredging channels.
1, Ningpo Yam rhizome: sweet, bitter and warm, entering liver, kidney and lung meridians; has the effects of dispelling pathogenic wind, removing dampness, relaxing muscles and tendons, dredging collaterals, promoting blood circulation and relieving pain.
White peony root: sour, bitter and slightly cold, entering liver and spleen meridians; has the functions of nourishing blood, regulating menstruation, nourishing liver and relieving pain.
The prescription of the invention has definite compatibility, and the medicinal ingredients complement each other; wherein, the antler has the salty and warm taste and has the functions of entering liver and kidney meridians, warming kidney yang, strengthening bones and muscles, promoting blood circulation and reducing swelling, and is a monarch drug. Achyranthes root, radix achyranthis bidentatae, picric acid and Ping enter liver channel, and have the functions of nourishing liver and kidney, strengthening bones and muscles, removing blood stasis and stimulating the menstrual flow; the orientvine, bitter in shape, enters liver and spleen channels, and has the functions of expelling wind-damp and dredging channels and collaterals; the two cornu cervi can be used as ministerial drugs for treating soreness of waist and knees, weakness of bones and muscles, joint swelling, limb paralysis and other symptoms caused by bone impediment. The yam rhizome is sweet, bitter and warm, enters liver, kidney and lung channels, and has the functions of dispelling wind and removing dampness, relaxing muscles and tendons, dredging collaterals, promoting blood circulation and relieving pain; white peony root, radix paeoniae alba is sour, bitter and slightly cold, enters liver and spleen channels, and has the functions of nourishing blood, regulating menstruation, softening liver and relieving pain; the two drugs are assistant drugs, and are used for treating arthroncus, pain, numbness, limb spasm and pain of bone impediment. Niu xi is used as a guiding drug for directing blood downward. The traditional Chinese medicine composition has the effects of tonifying liver and kidney, strengthening bones and muscles, dispelling wind and removing dampness, promoting blood circulation and relieving pain, and has the effects of dredging arthralgia and activating collaterals, and removing blood stasis and stimulating the menstrual flow.
The invention has the beneficial effects.
The invention is based on the pathogenesis of liver and kidney deficiency and meridian obstruction, the classical meridian prescription of 'antler' pills, and the clinical experience of orthopedic specialists in affiliated hospitals of Liaoning traditional Chinese medicine university for many years in treating osteoarthritis, the invention is prepared by continuously adjusting the medicine dosage and adding flavor, and the summarized clinical experience formula; is an effective medicine prepared from pure traditional Chinese medicines and used for treating pain, swelling, numbness, difficulty in bending and stretching, joint stiffness, deformity and other diseases caused by osteoarthritis and proliferative arthritis; and animal experiments prove that the traditional Chinese medicine composition has obvious analgesic and anti-inflammatory effects.
Antler pills come from Song, Ting and Ji Sheng Fang. The book carrier comprises: for bone deficiency, facial swelling, dark and dirty, lingering pain, collapse of teeth due to qi deficiency, lumbago, backache and even spittle. Two horn and two horn, and two radix cyathulae halves. Grinding into fine powder, and making into honeyed pill, such as Firmiana simplex seed, seventy pills are taken each time, and hollow salt soup is taken.
The liver and kidney tonifying antler, tendon and bone strengthening, liver and kidney meridian entering, liver and kidney meridian strengthening, achyranthes root, liver and kidney strengthening, bone and bone strengthening, stasis removing, meridian dredging, caulis sinomenii rheumatism dispelling, meridian dredging, collateral dredging, bone pain failing to stretch, blood gas dispelling, heat and fire rotting, abortion recorded in the Shennong herbal channel, wherein achyranthes root, caulis sinomenii, wind-cold-dampness arthralgia dispelling, tendon relaxing and blood activating, bone and marrow benefiting, wind disease weakness and weakness, powerful and partial waste syndrome, long-term taking, great strange function, rhizoma dioscoreae nipponicae wind-dampness dispelling, collateral dredging, blood activating, blood nourishing, white paeony root, radix paeoniae alba, liver softening, liver and pain relieving, tendon and muscle and bone swelling, numbness, tendon and bone deficiency relieving, liver and kidney damage, liver and kidney pain relieving, liver and kidney damage, liver and kidney meridian obstruction, liver and kidney damage, liver and spleen and kidney damage, liver and spleen and kidney damage, pain relieving, inflammation and spleen and kidney damage, inflammation, pain, liver and kidney damage, liver and spleen and kidney damage, liver and kidney damage, pain relieving, inflammation and kidney damage, liver and spleen and kidney damage, inflammation and spleen and kidney damage, pain, liver and spleen and kidney damage, inflammation and kidney damage, pain relieving, inflammation and spleen and kidney damage, inflammation and kidney damage, pain, inflammation and spleen and kidney damage, pain relieving, and kidney damage, inflammation, pain relieving, and kidney damage, and spleen and kidney damage.
Drawings
FIG. 1 shows the result of HE staining of rat osteoarthritis caused by the detachment of anterior cruciate ligament by the traditional Chinese medicine preparation of the invention.
FIG. 2 shows Masson's staining results of the Chinese medicinal preparation of the present invention on anterior cruciate ligament detached rat osteoarthritis.
FIG. 3 shows the staining result of toluidine blue staining of rats with dissociated anterior cruciate ligament by the Chinese herbal preparation of the present invention.
FIG. 4 shows the staining result of the Chinese medicinal preparation of the invention on rat osteoarthritis HE induced by papain and cysteine.
FIG. 5 shows the result of Masson's staining of rat osteoarthritis induced by papain and cysteine in the Chinese medicinal preparation of the present invention.
FIG. 6 shows the staining result of papain + cysteine induced toluidine blue in rat osteoarthritis by the Chinese medicinal preparation of the present invention.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1.
The traditional Chinese medicine mixture comprises the following raw material medicines in parts by weight: 10 parts of antler, 12 parts of achyranthes root, 10 parts of orientvine, 15 parts of Ningpo yam rhizome and 10 parts of fried white paeony root.
The preparation method of the traditional Chinese medicine mixture comprises the following steps: weighing the raw materials according to the weight part ratio, adding water for decoction, adding water 8 times of the total weight of the medicine, decocting for 2 times, 1.5 hours each time, filtering, combining the filtrates, and concentrating to one fifth of the volume of the original mixture.
Firstly, an experimental method.
The protective effect of the traditional Chinese medicine preparation on rat osteoarthritis caused by anterior cruciate ligament detachment is researched.
1. Grouping and molding.
Taking 112 rats 180-220 g, half male and female, 16 rats in each group, randomly dividing the rats into 7 groups, wherein the groups are respectively as follows: (1) normal control group: distilled water 1ml/100 g/d; (2) model group, distilled water 1ml/100 g/d; (3) the low dose group of the chinese medicinal formulation of example 1: 5g crude drug/kg/d; (4) dosage groups in the chinese medicinal preparation of example 1: 15g crude drug/kg/d; (5) the high dose group of the chinese herbal preparation of example 1: 50g crude drug/kg/d; (6) traditional Chinese medicine positive control group: bone-strengthening joint pill, 1.2 g/kg/d; (7) positive control group of western medicines: diacerein, 0.01 g/kg/d.
The molding method comprises the following steps: fixing the rat on a wood board in a supine position, cutting off leg hair in a region of 1cm around the knee joint cavity to be injected, and sterilizing by complexing iodine; opening the joint cavity of the right knee at the inner side beside the patella layer by layer, pushing the patella to the outer side to dislocate the patella, enabling the knee joint to be in the maximum buckling state, exposing the joint cavity, and cutting off the anterior cruciate ligament; the normal control group does not cut the anterior cruciate ligament after opening the joint cavity; each treatment group was administered with the drug administered to each group for 8 weeks, gavage, 2 times/day.
2. And (5) observing the knee joint surface damage degree score under a light microscope.
At 24h after 8 weeks of administration, the experimental animals were sacrificed under total anesthesia, the knee joints were separated, the articular surfaces were exposed, and observation was performed to observe the integrity and damage degree of the articular surfaces, with the following scoring criteria: the 0 minute-joint surface is smooth and regular in color; 1 minute-the joint surface is rough, has small cracks and is dark in color; 2 min-cartilage defect deep to cartilage middle layer; 3 min-the formation of ulcer of the articular surface, the cartilage defect reaches the deep layer of cartilage; 4 min-cartilage exfoliation with the subchondral bone exposed; the results are shown in Table 1.
Table 1 evaluation of the degree of injury to the surface of the knee joint under light microscopy.
The result shows that the score of the isolated model group is higher than that of the normal control group, P is less than 0.01, which indicates that the model of the experimental model group is successfully modeled, each dosage group in example 1 has statistical significance compared with the isolated model group, P is less than 0.01, and the high dosage group in example 1 has statistical significance compared with the western medicine positive control drug diacerein, P is less than 0.05; therefore, the traditional Chinese medicine preparation has a protective effect on knee arthritis of rats with detached anterior cruciate ligaments.
3. A pathological morphology detection method.
Cutting a specimen, immediately adding 10% formaldehyde for fixation for 48h, then soaking in 10% formic acid solution, and continuously decalcifying for 12d until the tissue is completely decalcified; soaking in distilled water, dehydrating, clearing, waxing, embedding, slicing to 6 μm, preserving, HE, Masson, and toluidine blue staining, and grading.
3.1HE staining: the slices are deparaffinized by gradient xylene, stained by hematoxylin for 5min, washed by water, stained by eosin for 5min, washed by water, transparent xylene and sealed by neutral gum.
The results are shown in FIG. 1, wherein 1-normal control group, 2-isolated model group, 3-bone strengthening joint pill group, 4-diacerein group, 5-low dose group, 6-medium dose group, and 7-high dose group.
3.2Masson staining: fixing by a Bounin fixing solution for 48h, decalcifying by 30% formic acid for 48h, processing all samples according to a conventional paraffin slicing procedure, slicing by the thickness of 7um, transferring the slices to water washing, immersing in an acetic acid-sodium acetate buffer solution with the pH of 6.0 for 30min (37 ℃), digesting by 0.5% hyaluronidase for 6h, washing by water, slicing by a calcium chloride solution of 2mol/L for 48h, washing by water, performing Masson dyeing, mixing Weigart hematoxylin A and Weigart hematoxylin B in equal proportion, dyeing for 10min, washing by distilled water for 1min, dyeing by a Masson composite dyeing solution for 15min, washing by distilled water slightly, treating by 1% phosphomolybdic acid solution for 5min, re-dyeing by aniline blue dyeing solution for 5min, treating by 1% glacial acetic acid for 1min, and dehydrating and sealing the transparent slices.
The results are shown in FIG. 2, wherein 1-normal control group, 2-isolated model group, 3-bone strengthening joint pill group, 4-diacerein group, 5-low dose group, 6-medium dose group, and 7-high dose group.
3.3 toluidine blue staining: and (3) carrying out 30min on toluidine blue staining solution, washing with tap water, differentiating glacial acetic acid until nucleus is clear, not easily causing over-differentiation if differentiation time is too long, washing with tap water again, airing, enabling xylene to be transparent, and sealing with neutral gum.
The results are shown in FIG. 3, wherein 1-normal control group, 2-isolated model group, 3-bone strengthening joint pill group, 4-diacerein group, 5-low dose group, 6-medium dose group, and 7-high dose group.
The staining according to the above three methods was performed to score cartilage structure, quantity, matrix, etc., with the following scoring criteria, see table 2.
Table 2 scoring criteria for cartilage structure, quantity, matrix, etc.
The scoring results are shown in table 3.
Table 3HE, Masson, toluidine blue staining pathology score and composite score results.
Comparison with Normal group*P<0.05,**P<0.01, compared with the set of disconnection models#P<0.05,##P<0.01, Delta P compared with Zhuanggu arthrosis pill group<0.05,ΔΔP<0.01。
The three staining scores and the comprehensive score of the isolated model group are higher than those of the normal control group, and all P is less than 0.01, which indicates that the model of the experimental model group is successfully made, the comprehensive score of each dosage group in example 1 has statistical significance compared with the isolated group, all P is less than 0.01, and the low and medium dosage groups have statistical significance compared with the bone strengthening joint pill group, and P is less than 0.01. Therefore, the traditional Chinese medicine preparation has a protective effect on knee arthritis of rats with detached anterior cruciate ligaments.
4. Example 1 Effect on serum IL-1 β, TNF- α, PGE2 levels in rats with dissociated anterior cruciate ligament.
24h after 8 weeks of administration, the experimental animals were completely anesthetized, the femoral artery was bled, 3000 rpm and 10min were performed, the supernatant was collected for experiment, the operation was performed according to the kit instructions of IL-1 β, TNF- α and PGE2, and the experimental results are shown in Table 4.
TABLE 4 results of testing the contents of IL-1 β, TNF- α and PGE2 in rat serum.
| Group of | Dosage (g/kg) | Number only | IL-1β(ng/L) | TNF-α(ng/L) | PGE2(ng/L) |
| Normal group | --- | 10 | 15.18±2.30 | 67.83±13.27 | 605.25±52.53 |
| Separation group | --- | 10 | 19.72±3.68** | 92.45±19.23** | 668.90±71.71* |
| |
5 | 10 | 14.27±3.17## | 81.49±17.67 | 595.02±59.18# |
| Middle dose group | 15 | 10 | 16.36±3.52## | 80.50±14.84 | 653.98±104.08 |
| High dose group | 50 | 10 | 15.78±2.48## | 91.31±16.02 | 648.78±80.22 |
| Bone-strengthening joint pill group | 1.2 | 10 | 15.94±2.45## | 89.59±16.05 | 643.61±75.71 |
| Diacerein group | 0.01 | 10 | 15.07±2.77## | 92.28±24.02 | 574.63±67.47# |
The results show that the levels of IL-1 β, TNF- α and PGE2 in the model group are all increased compared with the normal group, which indicates that the model of the experiment model is successfully modeled, and example 1 has a reducing effect on serum IL-1 β, P <0.01, and has no significant content on serum TNF- α and PGE2, and only the low dose group reduces the serum PGE2 content, and has statistical difference, wherein P < 0.05. compared with the normal group, P <0.05, P <0.01, compared with the model group, delta P <0.05, and delta P < 0.01.
(II) the traditional Chinese medicine preparation provided by the invention is used for researching the protective effect of papain and cysteine on rat osteoarthritis.
1 grouping and molding.
Taking 112 rats 180-220 g, half of each male rat and half of each female rat, and randomly dividing the rats into 7 groups, wherein the groups are respectively as follows: (1) normal control group: distilled water 1ml/100 g/d; (2) model group, distilled water 1ml/100 g/d; (3) example 1 low dose group of chinese medicinal preparation: 5g crude drug/kg/d; (4) example 1 medium dose group of chinese medicinal preparation: 15g crude drug/kg/d; (5) example 1 high dose group of chinese medicinal preparation: 50g crude drug/kg/d; (6) traditional Chinese medicine positive control group: bone-strengthening joint pill, 1.2 g/kg/d; (7) positive control group of western medicines: diacerein, 0.01 g/kg/d.
The molding method comprises the following steps: preparing a molding solution of 4% of papain and 0.3M cysteine, fixing the molding solution on a wood board in a supine position, cutting off leg hair in a region of 1cm around the knee joint cavity to be injected, and complexing iodine for disinfection. The knee joint of the rat is bent by 45 degrees, the knee eye at the outer edge of the extremely white patella tendon under the patella is taken as an insertion point, the needle is punctured towards the intercondylar fossa direction, the needle is retracted by 2mm after reaching the femoral condyle, and 0.1ml is slowly injected into the joint cavity. The normal control group was injected with an equal volume of saline. Each drug treatment group was administered continuously for 6 weeks, gavage, 2 times/day.
2. And (5) observing the knee joint surface damage degree score under a light microscope.
At 24h after 6 weeks of administration, the experimental animals were sacrificed under total anesthesia, the knee joints were separated, the articular surfaces were exposed, and observation was made to observe the integrity and damage degree of the articular surfaces, with the following scoring criteria: the 0 minute-joint surface is smooth and regular in color; 1 minute-the joint surface is rough, has small cracks and is dark in color; 2 min-cartilage defect deep to cartilage middle layer; 3 min-the formation of ulcer of the articular surface, the cartilage defect reaches the deep layer of cartilage; 4 fen-cartilage exfoliation with exposed subchondral bone. The results are shown in Table 5.
Table 5 evaluation of the degree of injury to the surface of the knee joint under light microscopy.
The results showed that the papain + cysteine group scored higher than the normal control, P<0.01, which shows that the model of the experiment is successfully modeled, and the dosage groups in example 1 have statistical significance compared with the papain + cysteine group, P<0.01, the bone-strengthening joint pill group of the traditional Chinese medicine positive control medicament and the diacerein group of the western medicine positive control medicament have statistical significance compared with the papain and cysteine group, P<0.05. The invention has the protection effect on rat gonarthritis induced by papain and cysteine. Comparison with Normal group*P<0.05,**P<0.01, compared to the papain + cysteine group#P<0.05,##P<0.01。
3. A pathological morphology detection method.
Immediately putting 10% formaldehyde into the cut specimen for fixation for 48 hours, then soaking the specimen in 10% formic acid solution, and continuously decalcifying for 12 days until the tissue is completely decalcified. Soaking in distilled water, dehydrating, clearing, waxing, embedding, slicing to 6 μm, preserving, HE, Masson, and toluidine blue staining, and grading.
3.1HE staining: the slices are deparaffinized by gradient xylene, stained by hematoxylin for 5min, washed by water, stained by eosin for 5min, washed by water, transparent xylene and sealed by neutral gum.
The results are shown in FIG. 4, where 1-normal control group; 2-papain + cysteine group; 3-group of Zhuanggu arthrosis pills; 4-diacerein group; 5-low dose group; 6-middle dose group; 7-high dose group.
3.2Masson staining: fixing by a Bounin fixing solution for 48h, decalcifying by 30% formic acid for 48h, processing all samples according to a conventional paraffin slicing procedure, slicing by the thickness of 7um, transferring the slices to water washing, immersing in an acetic acid-sodium acetate buffer solution with the pH of 6.0 for 30min (37 ℃), digesting by 0.5% hyaluronidase for 6h, washing by water, slicing by a calcium chloride solution of 2mol/L for 48h, washing by water, performing Masson dyeing, mixing Weigart hematoxylin A and Weigart hematoxylin B in equal proportion, dyeing for 10min, washing by distilled water for 1min, dyeing by a Masson composite dyeing solution for 15min, washing by distilled water slightly, treating by 1% phosphomolybdic acid solution for 5min, re-dyeing by aniline blue dyeing solution for 5min, treating by 1% glacial acetic acid for 1min, and dehydrating and sealing the transparent slices.
The results are shown in FIG. 5, where 1-normal control group; 2-papain + cysteine group; 3-group of Zhuanggu arthrosis pills; 4-diacerein group; 5-low dose group; 6-middle dose group; 7-high dose group.
3.3 toluidine blue staining: and (3) carrying out 30min on toluidine blue staining solution, washing with tap water, differentiating glacial acetic acid until nucleus is clear, not easily causing over-differentiation if differentiation time is too long, washing with tap water again, airing, enabling xylene to be transparent, and sealing with neutral gum.
The results are shown in FIG. 6, where 1-normal control group; 2-papain + cysteine group; 3-group of Zhuanggu arthrosis pills; 4-diacerein group; 5-low dose group; 6-middle dose group; 7-high dose group.
The staining according to the above three methods was performed to score cartilage structure, quantity, matrix, etc., with the scoring criteria as shown in table 6.
Table 6 scoring criteria for cartilage structure, quantity, matrix, etc.
The scoring results are shown in table 7.
Table 7HE, Masson, toluidine blue staining pathology scores and composite score results.
Comparison with Normal group*P<0.05,**P<0.01, comparison with model group#P<0.05,##P<0.01。
The three staining scores and the comprehensive score of the papain and cysteine group are higher than those of a normal control group, and the P is less than 0.01, which shows that the modeling of the experimental model group is successful, the toluidine blue and the comprehensive score of each dosage group in example 1 have statistical significance compared with the papain and cysteine group, the P is less than 0.01, the HE staining score of the middle and high dosage group compared with the papain and cysteine group has statistical significance, and the P is less than 0.01. The Chinese and western positive medicines have statistical significance in comparison of each dyeing score with the papain and cysteine group, P is less than 0.05, and P is less than 0.01, so that the traditional Chinese medicine preparation disclosed by the invention has a protection effect on the gonarthritis of rats in the papain and cysteine group.
4. Example 1 Effect on papain + cysteine induced levels of IL-1 β, TNF- α, and PGE2 in rat serum from gonarthritis.
24h after 6 weeks of administration, the experimental animals were completely anesthetized, the femoral artery was bled, 3000 rpm and 10min were taken, the supernatant was taken for experiment, the operation was performed according to the instructions of IL-1 β, TNF- α and PGE2 kits, and the experimental results are shown in Table 8.
TABLE 8 results of the determination of IL-1 β, TNF- α and PGE2 in rat serum.
The results show that the contents of papain and cysteine group IL-1 β and TNF- α are increased compared with the normal group, which indicates that the experimental model is successfully modeled, the medium-dose group has the function of reducing serum IL-1 β, and P is<0.01, the contents of TNF- α and PGE2 in serum are not significant, and compared with the papain and cysteine group, the content of IL-1 β in serum is reduced, and P is added in the middle dose group<0.05, the content of IL-1 β in serum was reduced in the middle dose group compared with the papain + cysteine group, and P was found to be present in the middle dose group<0.05, the high dose group had a reduced level of serum TNF- α, P, as compared to the papain + cysteine group<0.01, and has reduced serum TNF- α content and reduced P content compared with bone strengthening joint pill group<0.05. Comparison with normal group<0.05,**P<0.01, comparison with model group#P<0.05,##P<0.01, Delta P compared with Zhuanggu arthrosis pill group<0.05,ΔΔP<0.01。
Example 2.
The traditional Chinese medicine preparation comprises the following raw material medicines in parts by weight: 8 parts of antler, 8 parts of achyranthes root, 12 parts of orientvine, 13 parts of Ningpo yam rhizome and 8 parts of fried white paeony root.
Example 3.
The traditional Chinese medicine preparation comprises the following raw material medicines in parts by weight: 13 parts of antler, 10 parts of achyranthes root, 8 parts of orientvine, 10 parts of Ningpo yam rhizome and 13 parts of fried white paeony root.
Second, typical cases.
Case 1: some remained, male, 56 years old; the main complaints are: pain in the left knee joint was 1 year, aggravated for half a month. The symptoms are as follows: pain in the left knee joint, stabbing pain, fixed pain, dark tongue with white coating, and wiry pulse; no past history; no history of allergy; special examination: left knee swelling (-), medial and lateral joint space tenderness (+), motion restriction (+), patellar test (-), mazel sign (-), anterior and posterior drawer tests, and lateral stress tests (-); imaging examination: the right lateral side of the left knee joint shows that the medial joint gap is narrow to form bone hyperplasia with different degrees; traditional Chinese medicine diagnosis: arthralgia (blood stasis and qi stagnation syndrome), and Western diagnosis: left knee osteoarthritis; the therapeutic method comprises the following medicines: the preparation 14 of example 1, administered orally twice a day, was significantly improved in the pain symptoms of the left knee after administration.
Case 2: zhangyi, female, age 63; the main complaints are: swelling and pain of the right knee joint for 3 months, and aggravation with joint stiffness for 4 days; the symptoms are as follows: swelling and pain of right knee joint, soreness and weakness of waist and knees, red tongue with white coating, and deep and thready pulse; history of the past: the history of diabetes is 5 years, the metformin sustained-release tablet is taken by self, and the blood sugar is controlled stably; no history of allergy; special examination: swelling (+) of right knee, tenderness (+) of lateral joint clearance, restricted movement (+), patellar floating test (-), Lei Mai's sign (-), front and rear drawer test and lateral stress test (-), functional movement of right five toes and normal feeling of skin; traditional Chinese medicine diagnosis: western diagnosis of arthralgia syndrome and diabetes (liver and kidney deficiency syndrome): right knee osteoarthritis type 2 diabetes; the therapeutic method comprises the following medicines: the preparation of example 2, 14 doses, taken twice a day, reduced the pain symptoms in the right knee after administration, and 14 doses, the symptoms were significantly improved compared to the former.
Case 3: liu somebody, male, age 59; the main complaints are: pain in the knee joints is 6 months and aggravated for 2 days; the symptoms are as follows: pain of the double knee joints, stabbing pain, fixed pain, light and heavy day and night, dark tongue with white coating and wiry pulse; no past history; no history of allergy; special examination: the knee joint pain test method comprises the following steps of double knee swelling (-), left knee lateral joint gap tenderness (+), right knee medial joint gap tenderness (+), limited movement (+), a floating patella test (-), Maishi sign (-), a front and back drawer test and a lateral stress test (-), double-foot five-toe functional movement and skin feeling normal, and peripheral blood circulation good. Imaging examination: the right lateral parts of the double knee joints show that the medial intercondylar ridge of the left knee becomes sharp and the suprapatellar ligament is ossified; the lateral gap of the right knee joint is narrowed to form bone hyperosteogeny with different degrees; traditional Chinese medicine diagnosis: arthralgia (blood stasis and qi stagnation syndrome), and Western diagnosis: the prescription for treating the knee osteoarthritis comprises the following components: the formulation of example 3 was administered orally in 20 doses twice daily. After the medicine is taken, the pain symptom of the left knee is relieved, the improvement of the right knee is not obvious, and the pain symptom of the right knee is obviously improved compared with the former pain symptom after the medicine is taken for 15 doses.
Case 4: strictly, female, age 53, chief complaints: pain in the waist, restricted movement for 3 years, aggravated for 10 days. The symptoms are as follows: soreness and weakness in the lower back, weakness in the legs and knees, aggravated by fatigue, recurrent attacks, pale tongue, deep and thready pulse. No past history; no history of allergy; special examination: straightening physiological curvature of the waist, tendering (+) at the 4-sacrum 1 spinous process and 1.5cm lateral opening of the waist, 50 degrees (+) of a left straight leg elevation test, 70 degrees (-) of a right straight leg elevation test, a strengthening test (-), a sciatic nerve traction test (-), a '4' test (-), a pasteurism (-), normal muscle strength of double lower limbs, normal skin sensation and normal function and activity of five toes. Imaging examination: lumbar CT indicates lumbar 4-5, lumbar 5-sacral 1 disc herniation. Traditional Chinese medicine diagnosis: lumbago (liver and kidney deficiency), and Western diagnosis: prolapse of lumbar intervertebral disc. The therapeutic method comprises the following medicines: the formulation of example 1 was administered orally in 14 doses twice daily. After the medicine is taken, the symptoms are relieved, and the symptoms are obviously improved after the medicine is taken for 14 doses.
Claims (5)
1. A traditional Chinese medicine preparation for treating osteoarthritis is characterized by comprising the following raw material medicines in parts by weight: 8-13 parts of antler, 8-12 parts of achyranthes root, 8-12 parts of orientvine stem, 10-15 parts of Ningpo yam rhizome and 8-13 parts of fried white paeony root.
2. The traditional Chinese medicine preparation for treating osteoarthritis as claimed in claim 1, wherein the raw material medicines comprise, in parts by weight: 10 parts of antler, 12 parts of achyranthes root, 10 parts of orientvine, 15 parts of Ningpo yam rhizome and 10 parts of fried white paeony root.
3. The traditional Chinese medicine preparation for treating osteoarthritis as claimed in claim 1, wherein the raw material medicines comprise, in parts by weight: 8 parts of antler, 8 parts of achyranthes root, 12 parts of orientvine, 13 parts of Ningpo yam rhizome and 8 parts of fried white paeony root.
4. The traditional Chinese medicine preparation for treating osteoarthritis as claimed in claim 1, wherein the raw material medicines comprise, in parts by weight: 13 parts of antler, 10 parts of achyranthes root, 8 parts of orientvine, 10 parts of Ningpo yam rhizome and 13 parts of fried white paeony root.
5. The Chinese medicinal preparation for treating osteoarthritis according to any one of claims 1 to 4, wherein the preparation is a pill, powder, mixture, disintegrating tablet or dispersible tablet.
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| CN101444584A (en) * | 2008-12-31 | 2009-06-03 | 江西本草天工科技有限责任公司 | Muscle-and-tendon nourishing and bone-strengthening pharmaceutical preparation and preparation method thereof |
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| CN101444584A (en) * | 2008-12-31 | 2009-06-03 | 江西本草天工科技有限责任公司 | Muscle-and-tendon nourishing and bone-strengthening pharmaceutical preparation and preparation method thereof |
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