CN107080053A - A kind of heat-resistance type coating bioactive substance and preparation method thereof - Google Patents
A kind of heat-resistance type coating bioactive substance and preparation method thereof Download PDFInfo
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- CN107080053A CN107080053A CN201710169360.8A CN201710169360A CN107080053A CN 107080053 A CN107080053 A CN 107080053A CN 201710169360 A CN201710169360 A CN 201710169360A CN 107080053 A CN107080053 A CN 107080053A
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Abstract
Bioactive substance is coated with the invention provides a kind of heat-resistance type, including core and coatings, the core contains biological active component, carrier and adhesive, and the biological active component is protease or probiotics, and the carrier includes precipitated calcium carbonate and starch.Meanwhile, present invention also offers the preparation method that the heat-resistance type is coated with bioactive substance.The heat-resistance type is coated with bioactive substance by wrapping up one layer of coatings in biological active component outer surface, so that it is in the puffing process of field of fodder, completely cut off steam infiltration, so as to play heat-resisting function, be prevented effectively from or reduce the loss of active component in process in biological active component.
Description
Technical field
The invention belongs to technical field of bioengineering, and in particular to a kind of heat-resistance type coating bioactive substance and its preparation
Method.
Background technology
Bioactive substance such as protease, probiotics etc., are widely used in feed industry as feed addictive.
However, the activity of protease and probiotics is easily affected by temperature, during feed manufacturing, if directly by protease with
Probiotics is added in feed, and protease and probiotics can be affected by temperature and cause larger loss of activity.
Accordingly, it would be desirable to develop a kind of heat-resistance type coating bioactive substance, it is prevented effectively from or reduce active component and exist
Loss in process.
The content of the invention
The purpose of the present invention is to overcome the feed addictives such as existing protease and probiotics to be affected by temperature and easy
The problem of causing larger loss of activity.
Therefore, being coated with bioactive substance, including core and coatings, the core the invention provides a kind of heat-resistance type
Containing biological active component, carrier and adhesive, the biological active component is protease or probiotics, the carrier bag
Include precipitated calcium carbonate and starch.
Further, the packaging material that the coatings are used for palmitic acid, stearic acid, magnesium stearate in any one, it is described
The concentration of packaging material is 1~20%.
Further, the protease is acid protease, neutral proteinase or alkali protease；The probiotics
For clostridium butyricum, bacillus coagulans, bacillus licheniformis, streptococcus fecalis or enterococcus faecalis.
Further, the quality of the coatings is the 5~300% of core quality.
Further, the carrier quality is the 5~20% of biological active component quality.
Further, the mass ratio of precipitated calcium carbonate and starch is 9 in the carrier:1.
Further, described adhesive quality is the 0.1~10% of biological active component quality.
In addition, present invention also offers the preparation method that above-mentioned heat-resistance type is coated with bioactive substance, comprising the following steps：
1) preparation of core：Biological active component, carrier and adhesive are mixed in proportion, it is spray-dried that ultra micro is made
Biological active granulated, the as core of grain.
2) it is coated：By step 1) made from core be placed in fluid bed, regulation fluid bed EAT is 30~60 DEG C, is led to
The packaging material that coatings are used is injected in core surfaces by overspray mode, 30~60min of heating and thermal insulation under the conditions of 30~60 DEG C,
Produce heat-resistance type coating bioactive substance.
Further, the particle diameter of the core is 100~200 mesh；Particle diameter after core is coated is 20~120 mesh.
Further, the water content of the heat-resistance type coating bioactive substance is 1~8%.
Compared with prior art, beneficial effects of the present invention：
(1) this heat-resistance type coating bioactive substance that the present invention is provided in biological active component outer surface by wrapping up one layer
Coatings so that it is in the puffing process of field of fodder, isolation steam infiltration, so as to play heat-resisting function, be prevented effectively from or
Reduce the loss of active component in process in biological active component.
(2) this heat-resistance type coating bioactive substance that the present invention is provided uses palmitic acid, stearic acid or magnesium stearate
As coatings, under hydrochloric acid in gastric juice effect, its coatings can dissolve quickly discharges its coated biological active component, so that maximum journey
The activity of the utilization biological active component of degree.
Embodiment
The technical scheme in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, the common skill in this area
All other embodiment that art personnel are obtained under the premise of creative work is not made, belongs to the model that the present invention is protected
Enclose.
Embodiment 1：
Liquid basified protease 100kg is taken, 20% carrier is added, including precipitated calcium carbonate 18kg, starch 2kg, adhesive
HPMC 1kg, 150 DEG C of spray drying EAT, 70 DEG C of leaving air temp, atomizer frequency 200HZ obtains ultra micron biology living
Property particle 30kg.The biological active granulated 10kg of mesh ultra micron of 100 mesh -120 is taken to be coated, 30 DEG C of fluid bed EAT, bottom
The mode of spray is coated, and packaging material uses the stearic acid of concentration 20%, and the heating and thermal insulation 60min under the conditions of 30 DEG C, coating makes weight gain
5%, obtained coating bioactive substance particle diameter more than 80% is in the mesh of 100 mesh -120, and its water content is 1~8%.
To be coated with made from the present embodiment bioactive substance and not coated alkali protease 80 DEG C, it is 80% humidity, wet
Enzyme activity heat resistant test 10min is carried out in hot tank.Result of the test shows that the enzyme activity Retention for not being coated with alkali protease is 4.3%,
And the enzyme activity Retention that bioactive substance is coated with the present embodiment is 8.1%.
In addition, bioactive substance will be coated with hydrochloric acid in gastric juice made from the present embodiment with 1:1 mixing, at ambient temperature, bag
It can be completely dissolved by the coatings of bioactive substance in 1min.
Embodiment 2：
Liquid acid protease 100kg is taken, 5% carrier is added, including precipitated calcium carbonate 4.5kg, starch 0.5kg, adhesive
HPMC 0.1kg, 220 DEG C of spray drying EAT, 90 DEG C of leaving air temp, atomizer frequency 300HZ obtains ultra micron biological
Active particle 5kg.The biological active granulated 2kg of mesh ultra micron of 100 mesh -120 is taken to be coated, 60 DEG C of fluid bed EAT, bottom
The mode of spray is coated, and packaging material uses the palmitic acid of concentration 1%, and the heating and thermal insulation 30min under the conditions of 60 DEG C, coating makes weight gain
100%, obtained coating bioactive substance particle diameter more than 80% is in the mesh of 100 mesh -120, and its water content is 1~8%.
To be coated with made from the present embodiment bioactive substance and not coated acid protease 80 DEG C, it is 80% humidity, wet
Enzyme activity heat resistant test 10min is carried out in hot tank.Result of the test shows that the enzyme activity Retention for not being coated with acid protease is 3.8%,
And the enzyme activity Retention that bioactive substance is coated with the present embodiment is 36.9%.
In addition, bioactive substance will be coated with hydrochloric acid in gastric juice made from the present embodiment with 1:1 mixing, at ambient temperature, bag
It can be completely dissolved by the coatings of bioactive substance in 2min.
Embodiment 3：
Liquid neutral protease 100kg is taken, 20% carrier is added, including precipitated calcium carbonate 18kg, starch 2kg, adhesive
HPMC 1kg, 200 DEG C of spray drying EAT, 70 DEG C of leaving air temp, atomizer frequency 400HZ obtains ultra micron biology living
Property particle 30kg.The biological active granulated 10kg of mesh ultra micron of 100 mesh -120 is taken to be coated, 60 DEG C of fluid bed EAT, bottom
The mode of spray is coated, and packaging material uses the magnesium stearate of concentration 10%, and the heating and thermal insulation 50min under the conditions of 60 DEG C, coating makes weight gain
300%, obtained coating bioactive substance particle diameter more than 80% is in the mesh of 100 mesh -120, and its water content is 1~8%.
To be coated with made from the present embodiment bioactive substance and not coated neutral proteinase 80 DEG C, it is 80% humidity, wet
Enzyme activity heat resistant test 10min is carried out in hot tank.Result of the test shows that the enzyme activity Retention for not being coated with neutral proteinase is 3.2%,
And the enzyme activity Retention that enzyme granulate is coated with the present embodiment is 40.1%.
In addition, bioactive substance will be coated with hydrochloric acid in gastric juice made from the present embodiment with 1:1 mixing, at ambient temperature, bag
It can be completely dissolved by the coatings of bioactive substance in 4min.
Embodiment 4：
Clostridium butyricum liquid 100kg is taken, 10% carrier is added, including precipitated calcium carbonate 9kg, starch 1kg, adhesive HPMC
0.5kg, 180 DEG C of spray drying EAT, 60 DEG C of leaving air temp, atomizer frequency 300HZ obtains ultra micron bioactivity
Grain 26kg.The biological active granulated 10kg of mesh ultra micron of 100 mesh -120 is taken to be coated, 60 DEG C of fluid bed EAT, bottom spray
Mode is coated, and packaging material uses the palmitic acid of concentration 5%, and the heating and thermal insulation 50min under the conditions of 50 DEG C, coating makes weight gain 200%, obtained
The coating bioactive substance particle diameter more than 80% arrived is in the mesh of 100 mesh -120, and its water content is 1~8%.
To be coated with made from the present embodiment bioactive substance and not coated clostridium butyricum liquid 80 DEG C, it is 80% humidity, wet
Bioactivity heat resistant test 10min is carried out in hot tank.Result of the test shows that the active Retention for not being coated with clostridium butyricum liquid is
2.3%, and the enzyme activity Retention that enzyme granulate is coated with the present embodiment is 37.4%.
In addition, bioactive substance will be coated with hydrochloric acid in gastric juice made from the present embodiment with 1:1 mixing, at ambient temperature, bag
It can be completely dissolved by the coatings of bioactive substance in 3min.
Embodiment 5：
Bacillus coagulans liquid 100kg is taken, 20% carrier is added, including precipitated calcium carbonate 18kg, starch 2kg, adhesive
HPMC 1kg, 200 DEG C of spray drying EAT, 70 DEG C of leaving air temp, atomizer frequency 300HZ obtains ultra micron biology living
Property particle 32kg.The biological active granulated 15kg of mesh ultra micron of 100 mesh -120 is taken to be coated, 60 DEG C of fluid bed EAT, side
The mode of spray is coated, and packaging material uses the stearic acid of concentration 15%, and the heating and thermal insulation 50min under the conditions of 50 DEG C, coating makes weight gain
250%, obtained coating bioactive substance particle diameter more than 80% is in the mesh of 100 mesh -120, and its water content is 1~8%.
To be coated with made from the present embodiment bioactive substance and not coated bacillus coagulans liquid 80 DEG C, it is 80% wet
Bioactivity heat resistant test 10min is carried out in degree, damp heat box.Result of the test shows, the activity of bacillus coagulans liquid is not coated with
Retention is 4.7%, and the enzyme activity Retention that enzyme granulate is coated with the present embodiment is 41.3%.
In addition, bioactive substance will be coated with hydrochloric acid in gastric juice made from the present embodiment with 1:1 mixing, at ambient temperature, bag
It can be completely dissolved by the coatings of bioactive substance in 4min.
Embodiment 6：
Bacillus licheniformis liquid 100kg is taken, 10% carrier is added, including precipitated calcium carbonate 9kg, starch 1kg, adhesive
HPMC 0.6kg, 200 DEG C of spray drying EAT, 70 DEG C of leaving air temp, atomizer frequency 300HZ obtains ultra micron biological
Active particle 28kg.The biological active granulated 10kg of mesh ultra micron of 100 mesh -120 is taken to be coated, 40 DEG C of fluid bed EAT,
The mode of top spray is coated, and packaging material uses the magnesium stearate of concentration 10%, and the heating and thermal insulation 60min under the conditions of 45 DEG C, coating increases particle
200% is weighed, obtained coating bioactive substance particle diameter more than 80% is in the mesh of 100 mesh -120, and its water content is 1~8%.
To be coated with made from the present embodiment bioactive substance and not coated bacillus licheniformis liquid 80 DEG C, it is 80% wet
Bioactivity heat resistant test 10min is carried out in degree, damp heat box.Result of the test shows, the activity of bacillus licheniformis liquid is not coated with
Retention is 3.2%, and the enzyme activity Retention that enzyme granulate is coated with the present embodiment is 39.6%.
In addition, bioactive substance will be coated with hydrochloric acid in gastric juice made from the present embodiment with 1:1 mixing, at ambient temperature, bag
It can be completely dissolved by the coatings of bioactive substance in 3min.
Embodiment 7：
Streptococcus fecalis liquid 100kg is taken, 20% carrier is added, including precipitated calcium carbonate 18kg, starch 2kg, adhesive HPMC
5kg, 220 DEG C of spray drying EAT, 90 DEG C of leaving air temp, atomizer frequency 400HZ obtains ultra micron biological active granulated
36kg.The biological active granulated 20kg of mesh ultra micron of 100 mesh -120 is taken to be coated, 40 DEG C of fluid bed EAT, the side of top spray
Formula is coated, and packaging material uses the stearic acid of concentration 20%, and the heating and thermal insulation 60min under the conditions of 30 DEG C, coating makes weight gain 300%, obtained
The coating bioactive substance particle diameter more than 80% arrived is in the mesh of 100 mesh -120, and its water content is 1~8%.
To be coated with made from the present embodiment bioactive substance and not coated streptococcus fecalis liquid 80 DEG C, it is 80% humidity, wet
Bioactivity heat resistant test 10min is carried out in hot tank.Result of the test shows that the active Retention for not being coated with streptococcus fecalis liquid is
4.8%, and the enzyme activity Retention that enzyme granulate is coated with the present embodiment is 43.4%.
In addition, bioactive substance will be coated with hydrochloric acid in gastric juice made from the present embodiment with 1:1 mixing, at ambient temperature, bag
It can be completely dissolved by the coatings of bioactive substance in 4min.
Embodiment 8：
Enterococcus faecalis liquid 100kg is taken, 20% carrier is added, including precipitated calcium carbonate 18kg, starch 2kg, adhesive HPMC
5kg, 220 DEG C of spray drying EAT, 90 DEG C of leaving air temp, atomizer frequency 400HZ obtains ultra micron biological active granulated
34kg.The biological active granulated 20kg of mesh ultra micron of 100 mesh -120 is taken to be coated, 40 DEG C of fluid bed EAT, the side of top spray
Formula is coated, and packaging material uses the palmitic acid of concentration 10%, and the heating and thermal insulation 60min under the conditions of 30 DEG C, coating makes weight gain 150%, obtained
The coating bioactive substance particle diameter more than 80% arrived is in the mesh of 100 mesh -120, and its water content is 1~8%.
To be coated with made from the present embodiment bioactive substance and not coated enterococcus faecalis liquid 80 DEG C, it is 80% humidity, wet
Bioactivity heat resistant test 10min is carried out in hot tank.Result of the test shows that the active Retention for not being coated with enterococcus faecalis liquid is
3.5%, and the enzyme activity Retention that enzyme granulate is coated with the present embodiment is 37.1%.
In addition, bioactive substance will be coated with hydrochloric acid in gastric juice made from the present embodiment with 1:1 mixing, at ambient temperature, bag
It can be completely dissolved by the coatings of bioactive substance in 2min.
In summary, this heat-resistance type coating bioactive substance that the present invention is provided passes through in biological active component appearance
Bread wraps up in one layer of coatings so that it is in the puffing process of field of fodder, isolation steam infiltration, so that heat-resisting function is played,
It is prevented effectively from or reduces the loss of active component in process in biological active component.
It is exemplified as above be only to the present invention for example, do not constitute the limitation to protection scope of the present invention, it is all
It is to be belonged to the same or analogous design of the present invention within protection scope of the present invention.
Claims (10)
1. a kind of heat-resistance type is coated with bioactive substance, it is characterised in that：Including core and coatings, the core contains biology
Active component, carrier and adhesive, the biological active component are protease or probiotics, and the carrier includes lightweight carbon
Sour calcium and starch.
2. heat-resistance type coating bioactive substance as claimed in claim 1, it is characterised in that：The packaging material that the coatings are used
For any one in palmitic acid, stearic acid, magnesium stearate, the concentration of the packaging material is 1~20%.
3. heat-resistance type as claimed in claim 1 is coated with bioactive substance, it is characterised in that：The protease is acidic protein
Enzyme, neutral proteinase or alkali protease；The probiotics be clostridium butyricum, bacillus coagulans, bacillus licheniformis,
Streptococcus fecalis or enterococcus faecalis.
4. heat-resistance type as claimed in claim 1 is coated with bioactive substance, it is characterised in that：The quality of the coatings is core
The 5~300% of material amount.
5. heat-resistance type as claimed in claim 1 is coated with bioactive substance, it is characterised in that：The carrier quality is biological living
The 5~20% of property constituent mass.
6. heat-resistance type as claimed in claim 5 is coated with bioactive substance, it is characterised in that：Precipitated calcium carbonate in the carrier
Mass ratio with starch is 9:1.
7. heat-resistance type as claimed in claim 1 is coated with bioactive substance, it is characterised in that：Described adhesive quality is biology
The 0.1~10% of active component quality.
8. the heat-resistance type as described in any one of claim 1~7 is coated with the preparation method of bioactive substance, it is characterised in that：
Comprise the following steps：
1) preparation of core：Biological active component, carrier and adhesive are mixed in proportion, it is spray-dried that ultra micro is made
Biological active granulated, the as core of grain；
2) it is coated：By step 1) made from core be placed in fluid bed, regulation fluid bed EAT is 30~60 DEG C, passes through spray
Penetrate mode and the packaging material that coatings are used is injected in core surfaces, 30~60min of heating and thermal insulation, is produced under the conditions of 30~60 DEG C
Heat-resistance type is coated with bioactive substance.
9. heat-resistance type as claimed in claim 8 is coated with the preparation method of bioactive substance, it is characterised in that：The core
Particle diameter is 100~200 mesh；Particle diameter after core is coated is 20~120 mesh.
10. heat-resistance type as claimed in claim 8 is coated with the preparation method of bioactive substance, it is characterised in that：The heatproof
The water content of type coating bioactive substance is 1~8%.
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| CN113699054A (en) * | 2020-05-20 | 2021-11-26 | 中粮生物科技股份有限公司 | Clostridium butyricum solid microbial inoculum and preparation method thereof |
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Effective date of registration: 20180824 Address after: 430074 5, lingjiashan Road, East Lake Development Zone, Wuhan, Hubei. Applicant after: Wuhan Sunhy Biology Co., Ltd. Address before: 641300 Anyue Industrial Park, Ziyang economic and Technological Development Zone, Sichuan Applicant before: Sichuan Xinyang Shanye Biotechnology Co., Ltd. Applicant before: Wuhan Sunhy Biology Co., Ltd. |
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Application publication date: 20170822 |