CN107079939A - A kind of biological pesticide rich in Tea Saponin - Google Patents
A kind of biological pesticide rich in Tea Saponin Download PDFInfo
- Publication number
- CN107079939A CN107079939A CN201710488255.0A CN201710488255A CN107079939A CN 107079939 A CN107079939 A CN 107079939A CN 201710488255 A CN201710488255 A CN 201710488255A CN 107079939 A CN107079939 A CN 107079939A
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- biological pesticide
- tea saponin
- rich
- kuh
- seng
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of biological pesticide rich in Tea Saponin, it is related to biopharmaceutical technology, it is prepared from by camellia seed meal, argy wormwood, Siberian cocklebur, kuh-seng, grass-leaved sweetflag, emulsifying agent, the antibacterial substance rich content such as Tea Saponin, flavone compound, organic acid, alkaloid in biological pesticide of the present invention, it is active high, fungistatic effect is excellent, it is notable to control of plant disease effect, and pollution-free noresidue, is extremely adapted in current full green non-pollution agricultural production in the prevention and control of plant diseases, pest control of vegetables, melon and fruit, paddy rice, fruit and upland grain.
Description
Technical field
The present invention relates to biopharmaceutical technology, particularly a kind of biological pesticide rich in Tea Saponin.
Background technology
Corps diseases are to cause one of principal element of crop production reduction, disease it is serious cause the significantly underproduction,
And many crops have been unable to continuous cropping plantation.Although the preventing and treating of chemical pesticide has certain effect, seriously polluted, and with length
Phase applies, many bacterial resistance occurred, causes patient's condition to rise.Meanwhile, because the amount of applying of medicament is larger, cause crop medicine occur
Evil and intoxicating phenomenon, have a strong impact on agricultural production, more seriously in grain, veterinary antibiotics and all agricultural product, all
There are a large amount of noxious materials to remain, very harmful to people, animal, people, animal intoxicating phenomenon occurs often, has directly threatened human health.
In recent years, international and domestic advocate energetically uses biological pesticide, develops green agriculture, replaces the chemical agriculture being also used extensively by present
Medicine.At present, the kind of biological pesticide mainly has：Jinggangmeisu, antibiotic in pyrimidine nucleoside class, Ningnanmycin, macro tetrolide, agriculture resist
120th, c-type creotoxin etc..
" biological pesticide for pollution-free vegetables and its system disclosed in Chinese patent (number of patent application is 200610134652.X)
Method ", is formulated by following raw materials by the weight ratio, triperygium wilfordii extractive 5-70, gelsemium elegan extract 5-10, tea seed cake element 1-
30th, solvent 50-75, emulsifying agent 10-20；Invention raw material Thunder God Teng is the insecticidal materials of China's folk tradition, crushed material or is carried
Take thing to have strong stomach toxicity to a variety of agricultural fruit tree pest insects such as cabbage caterpillar, diamondback moth, food refusal can be caused or anesthetic effect is played, wherein
The main component worked is wilfordine and triptolide；In gelsemium elegan containing Diterpenes, flavonoids, lignanoid,
The compound such as cumarin and other Phenylpropanoid Glycosides；The ethanol extract of graceful jessamine herb has good insecticidal activity to cabbage caterpillar.
The content of the invention
It is an object of the invention to provide a kind of efficient, wide spectrum, low toxicity, the biological pesticide of safety.
To achieve the above object, the technical measures that the present invention is taken are a kind of biological pesticides rich in Tea Saponin of invention,
It is to be prepared from by the raw material of following mass ratio：
Camellia seed meal 20-30, argy wormwood 10-15, Siberian cocklebur 5-8, kuh-seng 3-6, grass-leaved sweetflag 4-9, emulsifying agent 3-5.
The preparation method of the described biological pesticide rich in Tea Saponin, comprises the following steps：
（1）Camellia seed meal, argy wormwood, Siberian cocklebur, kuh-seng, grass-leaved sweetflag are dried at 45-50 DEG C respectively, its water content is less than 14%,
It is processed as 30-50 mesh powders；
（2）Camellia seed meal, argy wormwood, Siberian cocklebur, kuh-seng, Grassleaf Sweetflag Rhizome supercritical extract, extraction temperature 50- are taken by the mass ratio
60 DEG C, extracting pressure 20-30MPa, time 2-4h；
（3）By step（2）Gained extract is mixed with water in mass ratio 1: 500-800, adds emulsifying agent, through ultrasonic wave homogeneous,
It is filling；
The emulsifying agent is Span-80；The ultrasonic wave processing condition is：Time 10-15min, 50-60 DEG C of temperature, power
250-350W；
Beneficial effects of the present invention：
Rationally, the antibacterial substance rich content such as its Tea Saponin, flavone compound, organic acid, alkaloid is active for inventive formulation
Height, fungistatic effect is excellent, to control of plant disease effect significantly, and pollution-free noresidue, is extremely adapted to current full green
Used in Harmless agricultural production, and the biological pesticide being prepared by the method for the present invention out, its raw material active ingredient is carried
Take rate high, extraction rate is fast, saves material, can largely produce.
Embodiment
With reference to specific embodiment, the present invention is described in further detail.
Embodiment 1
Supercritical extract instrument used is HA221-50-06CO2Nantong Huaan supercritical extract company produces；
A kind of biological pesticide rich in Tea Saponin, is prepared from by following raw materials according：
Camellia seed meal 30g, argy wormwood 15g, Siberian cocklebur 8g, kuh-seng 6g, grass-leaved sweetflag 9g, emulsifying agent 5g.
The preparation method of the described biological pesticide rich in Tea Saponin, comprises the following steps：
（1）Camellia seed meal, argy wormwood, Siberian cocklebur, kuh-seng, grass-leaved sweetflag are dried at 50 DEG C respectively, make its water content less than 14%, plus
Work is 50 mesh powders；
（2）Camellia seed meal, argy wormwood, Siberian cocklebur, kuh-seng, Grassleaf Sweetflag Rhizome is taken to be placed in extractor by the quality, in extraction temperature
60 DEG C, extract 4h under extracting pressure 30MPa；
（3）By step（2）Gained extract is mixed with water in mass ratio 1: 500-800, adds emulsifying agent, through ultrasonic wave homogeneous,
It is filling；
The emulsifying agent is Span-80；The ultrasonic wave processing condition is：Time 15min, temperature 50 C, power 250W.
Embodiment 2
Biological pesticide bacteriostatic test of the present invention；
Test decoction：Control group, 500-600 times of liquid of commercially available 25% Prochloraz missible oil；Test group, biological pesticide of the present invention.
Test strain：Grape anthracnose strain, is provided by plant protection institute of Agricultural University Of Nanjing.
Test method
Grape anthracnose strain is inoculated into inclined-plane PDA culture medium, in 26 DEG C of incubated 7d or so, plane training is inoculated into
Support and cultivated on base, it is standby.
Aseptically, 5mL experiment decoctions and being cooled to of having melted of 45mL are added into the sterilized beakers of 100mL
50 DEG C or so of culture medium, after fully shaking up, flat board is made in the culture dish for pouring into diameter 9cm.With diameter 6mm card punch
The anthrax bacteria for cultivating 5d is beaten in colony edge and takes bacteria cake, pastille culture medium flat board center is inoculated in, per 1 piece of ware.Under normal temperature
Cultivate after 7d, colony diameter is measured with crossing method.Each processing is repeated 5 times.Bacteriostasis rate is calculated as follows：Bacteriostasis rate
=（Compare colony diameter-processing colony diameter）/（Compare colony diameter-bacteria cake diameter）× 100%.
Result of the test
| Control group | Test group | |
| Bacteriostasis rate | 93.56% | 97.32% |
As can be seen that both fungistatic effects are good, and biological pesticide effect of the present invention is better than commercially available agricultural chemicals, possesses good reality
With value.
Claims (2)
1. a kind of biological pesticide rich in Tea Saponin, it is characterised in that be prepared from by the raw material of following mass ratio：
Camellia seed meal 20-30, argy wormwood 10-15, Siberian cocklebur 5-8, kuh-seng 3-6, grass-leaved sweetflag 4-9, emulsifying agent 3-5.
2. the preparation method of the biological pesticide of Tea Saponin is rich in as claimed in claim 1, it is characterised in that including following step
Suddenly：
（1）Camellia seed meal, argy wormwood, Siberian cocklebur, kuh-seng, grass-leaved sweetflag are dried at 45-50 DEG C respectively, its water content is less than 14%,
It is processed as 30-50 mesh powders；
（2）Camellia seed meal, argy wormwood, Siberian cocklebur, kuh-seng, Grassleaf Sweetflag Rhizome supercritical extract, extraction temperature 50- are taken by the mass ratio
60 DEG C, extracting pressure 20-30MPa, time 2-4h；
（3）By step（2）Gained extract is mixed with water in mass ratio 1: 500-800, adds emulsifying agent, through ultrasonic wave homogeneous,
It is filling；
The ultrasonic wave processing condition is：Time 10-15min, 50-60 DEG C of temperature, power 250-350W.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710488255.0A CN107079939A (en) | 2017-06-23 | 2017-06-23 | A kind of biological pesticide rich in Tea Saponin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710488255.0A CN107079939A (en) | 2017-06-23 | 2017-06-23 | A kind of biological pesticide rich in Tea Saponin |
Publications (1)
| Publication Number | Publication Date |
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| CN107079939A true CN107079939A (en) | 2017-08-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710488255.0A Pending CN107079939A (en) | 2017-06-23 | 2017-06-23 | A kind of biological pesticide rich in Tea Saponin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112790198A (en) * | 2021-02-26 | 2021-05-14 | 桂林新概念农业科技开发有限公司 | Environment-friendly nontoxic biological agent |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102239894A (en) * | 2011-05-12 | 2011-11-16 | 刘宝玉 | Compound botanical agricultural bactericide and preparation method thereof |
| CN102484993A (en) * | 2010-12-02 | 2012-06-06 | 华南农业大学 | Synergistic pesticide composition of matrine and tea saponin |
| CN103621582A (en) * | 2013-12-14 | 2014-03-12 | 象州县科学技术情报研究所 | Drug for controlling bacterial leaf blight of rice, and production method of drug |
-
2017
- 2017-06-23 CN CN201710488255.0A patent/CN107079939A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102484993A (en) * | 2010-12-02 | 2012-06-06 | 华南农业大学 | Synergistic pesticide composition of matrine and tea saponin |
| CN102239894A (en) * | 2011-05-12 | 2011-11-16 | 刘宝玉 | Compound botanical agricultural bactericide and preparation method thereof |
| CN103621582A (en) * | 2013-12-14 | 2014-03-12 | 象州县科学技术情报研究所 | Drug for controlling bacterial leaf blight of rice, and production method of drug |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112790198A (en) * | 2021-02-26 | 2021-05-14 | 桂林新概念农业科技开发有限公司 | Environment-friendly nontoxic biological agent |
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Application publication date: 20170822 |
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