CN107079809A - A kind of selection of edible oil certain herbaceous plants with big flowers male sterile line - Google Patents
A kind of selection of edible oil certain herbaceous plants with big flowers male sterile line Download PDFInfo
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- CN107079809A CN107079809A CN201710390335.2A CN201710390335A CN107079809A CN 107079809 A CN107079809 A CN 107079809A CN 201710390335 A CN201710390335 A CN 201710390335A CN 107079809 A CN107079809 A CN 107079809A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
A kind of edible oil certain herbaceous plants with big flowers male-sterile line breeding method, male sterile line is found in sunflower colony, generation maternal plant bagging is obtained, the sunflower colony with objective trait is separately divided into multiple generation male parent groups, bagging is numbered, and one plant of male sterility is authorized in the pollen mixing of generation male parent group；The maternal seed plantation of a generation is obtained two godmother this group again, the seed plantation of generation male parent obtains two godfather this group, bagging numbering；Two one group of this male sterility percentage of godmother highest are chosen, two generation plant of this group previous generation's male parent are divided into multiple male parent groups, a maternal individual plant is authorized after each group collection pollen mixing；So repeatedly until sterile rate 80%, then with the pollination of 1 pair 1 of male parent, reach seed selection purpose.Beneficial effect:Maintainer searches scope greatly, and sample is more, and success rate is high, and the cycle is short, suitable for being kept across population, passes through the seed selection of 5-6 generations, you can provides excellent sunflower hybrid strain, significantly improves the yield and quality of sunflower, market prospects are very considerable.
Description
Technical field
The present invention relates to a kind of sunflower breeding method, i.e., a kind of selection of edible oil certain herbaceous plants with big flowers male sterile line, particularly
It is a kind of to enjoy selection of the standing grain wide-margin-white edible oil certain herbaceous plants with big flowers as the male sterile line parent of maintainer.
Background technology
Edible oil certain herbaceous plants with big flowers refers to its seed not only edible but also the sunflower that can be extracted oil, and is important oil crops and industrial crops.Will
The breed breeding of sunflower must just be performed by improving the plantation level of edible oil certain herbaceous plants with big flowers, found " one high three resists " i.e. high yield, disease and insect resistance, resisted
Lodging and the kind of premature senescence resistance.At present, sunflower breed breeding include preferably with the mode such as crossbreeding.It is therein preferably through
The excellent plant seed of field observation selection traits is used as seed.And crossbreeding is the remote edge parent by selecting merit
This, is hybridized, to utilize its heterosis, hybrid vigor.Due to sunflower male flower and female flower closely, emasculation is difficult, thus the product of sunflower
Planting seed selection needs to complete by male sterile line.From crop genetic rule, it there may be among the natural community of crop
Male sterile line plant and male sterile line plant can be made to produce offspring and the maintainer plant of male sterility characteristic is kept.
Wherein, the male flower of male sterile line plant is ruined, and is easier identification, and maintainer plant does not have obvious characteristic, is difficult to find.
Because male sterile line and maintainer are the plant of association, thus, it is many at present that male sterile line is found among same population
Maintainer.But, the maintainer in same population not necessarily has the objective trait that we need, and therefore, we are more desirable to energy
It is enough to find maintainer among other populations for possessing objective trait, to cultivate the female parent with merit.Obviously, this
Difficulty than finding maintainer among same population is bigger.
At present, the basic mode for finding maintainer is that the offspring of the normal plant around male sterile line plant is distinguished
Numbering bagging, then so that " offspring in the way of in the of 1 couple 1 " as male sterile line pollinates, then is observed into plant of future generation, remains in that
Male sterile plant, its previous generation pollination plant is that possible to be to maintain be plant.Pass through repeated examinations again, just can determine that just
Body.The factors such as storage, distance and variation due to maintainer, the cycle of this experiment is longer, and success rate is not high.
Maintainer is found around male sterile plants even so difficult, if " 1 pair of 1 " method is among other populations using this
Maintainer is found, its difficulty well imagines that the probability of success is very little.
In recent years, my preferably dual-purpose sunflower new varieties of a kind of edible oil " enjoying standing grain wide-margin-white ", the kind " one high three resists "
Character is protruded, and no matter in Production of Large Fields or the male parent as crossbreeding, achieves satisfied effect.On this basis, originally
People wishes that " enjoying standing grain wide-margin-white " can turn into the maintainer of other male sterile lines with objective trait, with the advantage of itself
It is joined among new female parent, is that the upgrading of China's sunflower production is made contributions.Obviously, using current " 1 pair 1 " of side
Method, the purpose for finding the maintainer among " enjoying standing grain wide-margin-white " is difficult to, it is, therefore, necessary to find, a kind of cycle is short, operate letter
Just, the high new way of success rate.
The content of the invention
It is an object of the invention to provide a kind of success rate height, the choosing for the sunflower self-mating system that the cycle is short, easy to operate, cost is low
Educate method.Further objective is that it is that specific male sterile line finds guarantor in same population, particularly different population to find a kind of
Hold the method for being.Offer one kind is provided and finds other sunflower populations among sunflower new varieties " enjoying standing grain wide-margin-white "
The method of the maintainer of male sterile line.
Above-mentioned purpose is realized by following technical scheme：A kind of selection of edible oil certain herbaceous plants with big flowers male sterile line, its feature
It is：Male sterile line plant is found in the colony of sunflower, gathering the plantation of its seed, to obtain multiple generation maternal plant baggings standby
With the sunflower colony with objective trait being separately divided into multiple generation male parent groups, group quantity and the maternal strain number of the generation
Identical, plant bagging, each group group number mixes the pollen of same male parent group during pollination, authorizes one plant of male sterile plants；
The seed of each generation male sterility maternal plant is planted respectively again and obtains multiple two godmother this group for observation, is adopted simultaneously
The seed for collecting each generation male parent group plants two godfather this group obtained as much, plant bagging, group numbering respectively；See
Two godmother's in-groups are examined, multiple male sterility maternal plants in male sterility percentage one group of highest are chosen, while this
Two generation plant of previous generation's male parent group of group are divided into multiple male parent groups, and each group authorizes institute after gathering each strain pollen mixing
Select the maternal individual plant of one plant of male sterility of group；Repeat the mode of the previous generation, until male sterility percentage reaches 80%, then with it is selected
1 pair of 1 pollination of male parent, until obtaining male sterility percentage 100%, and the male sterile line with stable objects character.
The male sterile line plant is selected from crop field and eats certain herbaceous plants with big flowers, and the maintainer male parent group is " enjoying standing grain wide-margin-white " sunflower.
Described is that the detailed process that male parent cultivates male sterile line is with " enjoying standing grain wide-margin-white "：
1. finding male sterile line individual plant in sunflower field, it is one to choose ripe seed plantation and obtain A plants of male sterile plants
Godmother's sheet, numbering bagging.In " enjoying standing grain wide-margin-white " the earth the inside, preferably AxB plants are generation male parent, are divided into A groups, every group B plants, compile
Number, bagging.During pollination, to one plant of maternal pollination after the pollen mixing of B plants of collections of every group of male parent.
The seed of 2.A plants of generation maternal plants is planted respectively obtains A groups two generations maternal plant, while plantation A groups one respectively
The seed of godfather's sheet obtains two generation paternal plants.Maternal plant developmental state is observed, male sterility percentage highest suite number is taken
Bagging, other each groups are abandoned.From this one group of related paternal plant numbering bagging to selected two godmother, other each groups are abandoned.
C plants of male sterile plants of selection are standby in selected two generations maternal plant, and selected two generations paternal plant is divided into C groups, numbers respectively.
During pollination, paternal pollen is collected in units of group, one plant of female parent is authorized after every group of pollen mixing.
The seed of 3.C strain two generations maternal plants is planted respectively obtains C group three generations maternal plants, while plantation C groups two respectively
The seed of godfather's sheet obtains C group three generations's paternal plants.The maternal developmental state of observation, takes male sterility percentage highest suite number set
Bag, other each groups are abandoned.From the one group paternal plant numbering bagging related to selected three generations female parent, other each group male parents are put
Abandon.D plants of male sterile plants of selection are standby in selected three generations maternal plant, and selected three generations's paternal plant is divided into D groups, compiles respectively
Number.Pollinate season, each strain pollen is collected in units of group, one plant of female parent is authorized after every group of pollen mixing.
The seed of 4.D plants of three generations maternal plants is planted respectively obtains D groups four generations maternal plant, while plantation D groups three respectively
The seed of godfather's sheet obtains D groups four generations paternal plant.The maternal developmental state of observation, takes male sterility percentage highest suite number set
Bag, other each group female parents are abandoned.From this one group of related paternal plant numbering bagging, other each group male parents to selected four godmother
Abandon.E plants of male sterile plants of selection are standby in selected four generations maternal plant, and selected four generations paternal plant is divided into E groups, respectively
Numbering.During pollination, each strain pollen is collected in units of group, one plant of female parent is authorized after every group of pollen mixing.
5. said process is repeated, when acquisition male sterility percentage is sufficiently large, then with maternal and 1 pair of 1 pollination of male parent,
Until male sterility percentage 100%, and during objective trait stabilization, obtain being available for the sunflower male sterile line and maintainer of crossbreeding.
Described is that the detailed process that male parent cultivates male sterile line is with " enjoying standing grain wide-margin-white "：
1. finding male sterile line individual plant in food certain herbaceous plants with big flowers field, it is one to choose ripe seed plantation and obtain 10 plants of male sterile plants
Godmother's sheet, numbering bagging.In " enjoying standing grain wide-margin-white " the earth the inside, preferably 500 plants are generation male parent, are divided into 10 groups, every group 50 plants,
Numbering, bagging.During pollination, to one plant of maternal pollination after the pollen mixing of 50 plants of collections of every group of male parent.
The seed of 2.10 plants of generation maternal plants is planted respectively obtains 10 group of two generation plant, 10 group of one godfather planted respectively
This seed obtains 10 group of two generation paternal plant.The maternal developmental state of observation, takes male sterility percentage highest suite number bagging,
Other each groups are abandoned.From to this related suite number bagging of selected two godmother, other each groups abandon.In selected two godmothers sheet
10 plants of male sterile plants are chosen in plant, selected 500 plants of two generations paternal plant is divided into 10 groups, numbered respectively.Pollinate season, receive
Collect each strain pollen, mixed in units of group, every group of pollen authorizes one plant of female parent.
The seed of 3.10 plant of two generation maternal plant is planted respectively obtains 10 groups of three generations maternal plants, and 10 group of two generation is planted respectively
The seed of male parent obtains 10 groups of three generations's paternal plants.Developmental state is observed, male sterility percentage highest suite number bagging is taken, its
He abandons each group female parent.From the one group paternal plant numbering bagging related to selected three generations female parent, other each groups are abandoned.Institute
10 plants of male sterile plants of selection in three generations maternal plant are selected, selected 500 plants of three generations's paternal plant is divided into 10 groups, numbered respectively.
During pollination, each strain pollen is collected, is mixed in units of group, every group of pollen authorizes one plant of female parent.
The seed of 4.10 plants of three generations maternal plants is planted respectively obtains 10 group of four generation maternal plant, and 10 groups of three generations are planted respectively
The seed of male parent obtains four generation paternal plants.The maternal developmental state of observation, takes male sterility percentage highest suite number bagging, its
He abandons each group.From to this related suite number bagging of selected four godmother, other each groups abandon.In this plant of selected four godmother
10 plants of male sterile plants are chosen in strain, selected 500 plants of four generations paternal plant is divided into 10 groups, numbered respectively.During pollination, collect each
Strain pollen, is mixed in units of group, and every group of pollen authorizes one plant of female parent.
5. said process is repeated, when reaching more than 80% to male sterility percentage, then with maternal and 1 pair of 1 pollination of male parent,
To male sterility percentage 100%, and during objective trait stabilization, obtain sunflower male sterile line and maintainer.
The beneficial effects of the invention are as follows:Maintainer searches that scope is big, and sample is more, and success rate is high, and the cycle is short, suitable for setting up
Two across population are or three be to assemble, and by the seed selection in 2-3 years 5-6 generations, can obtain a kind of male with excellent moral character
Sterile sunflower self-mating system and maintainer, and then excellent parent is provided for sunflower crossbreeding, significantly improve sunflower yield and
Quality, greatly improves the income of certain herbaceous plants with big flowers agriculture, promotes the development of related industry, and market prospects are very considerable.
Brief description of the drawings
Fig. 1 is the seed selection flow chart of second of embodiment.
Embodiment
The first embodiment：Present study has the purpose of three levels：One is that seed selection one kind is available for crossbreeding to use
Male sterile line and maintainer.Two be that selected male sterile line has desirable merit.Three be selected hero
The maintainer of property sterile line parent is produced preferably in another remote edge strain.In above-mentioned purpose, first purpose belongs to most basic
Operation, although the acquisition difficulty of male sterile line is very big, many professionals make great efforts lifetime and without being obtained, be still real
The lowest term of existing self pollination crop crossbreeding.Second purpose is a kind of higher pursuit, because finding male sterility
The difficulty of system is very big, and the character to male sterile line itself should not do higher requirement again, and obtain excellent by excellent male parent
Benign shape.The male sterile line that this experiment wishes obtained inherently has objective trait, and this is difficult to accomplish.3rd mesh
Difficulty it is bigger, due to being grown in around male sterile line plant the maintainer of male sterile line in natural environment, thus more
Preferably maintainer is searched among same plot.But, in order to pursue more preferably more merits, singly around plant even
It is inadequate that gene is introduced between same kind, thus hope finds maintainer in excellent remote edge germline.
In order to achieve the above object, this research selects a variety of different cultivars to carry out multigroup experiment, obtains a small amount of success
Combination.The present embodiment introduction is one of combination optimized.Its male sterile line plant system eats the crop field of certain herbaceous plants with big flowers from the U.S.
It is central to find.The maintainer is from my seed selection and among the dual-purpose sunflower kind of edible oil " enjoying standing grain wide-margin-white " through authorization name
Choose.
Described is that the detailed process that male parent cultivates male sterile line is with " enjoying standing grain wide-margin-white "：
1. finding male sterile line individual plant in sunflower field, it is one to choose ripe seed plantation and obtain A plants of male sterile plants
Godmother's sheet, numbering bagging.In " enjoying standing grain wide-margin-white " the earth the inside, preferably AXB plants are generation male parent, are divided into A groups, every group B plants, compile
Number, bagging.During pollination, to one plant of maternal pollination after the pollen mixing of B plants of collections of every group of male parent.Here the numerical value of B strains is bigger,
Selected scope is bigger, and the possibility for finding maintainer is bigger, but the algebraically of numerical value too conference increase separation.Synthesis is examined
Consider, we are proper between choosing 50-200 plants.
The seed of 2.A plants of generation maternal plants is planted respectively obtains A groups two generations maternal plant, while plantation A groups one respectively
The seed of godfather's sheet obtains two generation paternal plants.Here the quantity of A groups paternal plant can refer to foregoing index, but preferably upper
Seed choosing for each plant is complete.Maternal plant developmental state is observed, male sterility percentage highest suite number bagging is taken, other
Each group is abandoned.From this one group of related paternal plant numbering bagging to selected two godmother, other each groups are abandoned.In selected two generation
C plants of male sterile plants of selection are standby in maternal plant, and selected two generations paternal plant is divided into C groups, numbers respectively.During pollination, with
Group collects paternal pollen for unit, and one plant of female parent is authorized after every group of pollen mixing.
The seed of 3.C strain two generations maternal plants is planted respectively obtains C group three generations maternal plants, while plantation C groups two respectively
The seed of godfather's sheet obtains C group three generations's paternal plants.The maternal developmental state of observation, takes male sterility percentage highest suite number set
Bag, other each groups are abandoned.From the one group paternal plant numbering bagging related to selected three generations female parent, other each group male parents are put
Abandon.D plants of male sterile plants of selection are standby in selected three generations maternal plant, and selected three generations's paternal plant is divided into D groups, compiles respectively
Number.During pollination, each strain pollen is collected in units of group, one plant of female parent is authorized after every group of pollen mixing.
The seed of 4.D plants of three generations maternal plants is planted respectively obtains D groups four generations maternal plant, while plantation D groups three respectively
The seed of godfather's sheet obtains D groups four generations paternal plant.The maternal developmental state of observation, takes male sterility percentage highest suite number set
Bag, other each group female parents are abandoned.From this one group of related paternal plant numbering bagging, other each group male parents to selected four godmother
Abandon.E plants of male sterile plants of selection are standby in selected four generations maternal plant, and selected four generations paternal plant is divided into E groups, respectively
Numbering.During pollination, each strain pollen is collected in units of group, one plant of female parent is authorized after every group of pollen mixing.
5. said process is repeated, when acquisition male sterility percentage is sufficiently large, then with maternal and 1 pair of 1 pollination of male parent.
Mentioned here sufficiently large, its span is wider, but too low ratio regular meeting extends follow-up separation process.Consider, one
As be not less than 50%, be preferred with 80%, then carry out 1 pair 1 of selection process, until male sterility percentage 100%, and objective trait is steady
Regularly, sunflower male sterile line and maintainer are obtained.
The male sterile line cultivated has that the strain of maintainer " enjoying standing grain wide-margin-white " is short, resistant to lodging, seed is good, disease-resistant, anti-
The merits such as drought, with plurality of target breed combination breeding, receive satisfied effect, excellent compared with existing common variety
Gesture highly significant, volume increase 15-20%, the deep favorable comment by test block certain herbaceous plants with big flowers agriculture.
Second of embodiment：On the basis of the first embodiment, suitable parameter is selected, can be in the specification of restriction
Complete Breeding Process.Recommend a specific operation process with reference to Fig. 1：
1. finding male sterile line individual plant in food certain herbaceous plants with big flowers field, it is one to choose ripe seed plantation and obtain 10 plants of male sterile plants
Godmother's sheet, respectively numbering bagging.In " enjoying standing grain wide-margin-white " the earth the inside, preferably 500 plants are generation male parent, are divided into 10 groups, every group 50
Strain, respectively numbering bagging.During pollination, to one plant of maternal pollination after the pollen mixing of 50 plants of collections of every group of male parent.
The seed of 2.10 plants of generation maternal plants is planted respectively obtains 10 group of two generation plant, 10 group of one godfather planted respectively
This seed obtains 10 group of two generation paternal plant.The maternal developmental state of observation, takes male sterility percentage highest suite number bagging,
Other each groups are abandoned.From to this related suite number bagging of selected two godmother, other each groups abandon.In selected two godmothers sheet
10 plants of male sterile plants are chosen in plant, selected 500 plants of two generations paternal plant is divided into 10 groups, numbered respectively.Pollinate season, receive
Collect each strain pollen, mixed in units of group, every group of pollen authorizes one plant of female parent.
The seed of 3.10 plant of two generation maternal plant is planted respectively obtains 10 groups of three generations maternal plants, and 10 group of two generation is planted respectively
The seed of male parent obtains 10 groups of three generations's paternal plants.Developmental state is observed, male sterility percentage highest suite number bagging is taken, its
He abandons each group female parent.From the one group paternal plant numbering bagging related to selected three generations female parent, other each groups are abandoned.Institute
10 plants of male sterile plants of selection in three generations maternal plant are selected, selected 500 plants of three generations's paternal plant is divided into 10 groups, numbered respectively.
During pollination, each strain pollen is collected, is mixed in units of group, every group of pollen authorizes one plant of female parent.
The seed of 4.10 plants of three generations maternal plants is planted respectively obtains 10 group of four generation maternal plant, and 10 groups of three generations are planted respectively
The seed of male parent obtains four generation paternal plants.The maternal developmental state of observation, takes male sterility percentage highest suite number bagging, its
He abandons each group.From to this related suite number bagging of selected four godmother, other each groups abandon.In this plant of selected four godmother
10 plants of male sterile plants are chosen in strain, selected 500 plants of four generations paternal plant is divided into 10 groups, numbered respectively.During pollination, collect each
Strain pollen, is mixed in units of group, and every group of pollen authorizes one plant of female parent.
Be that can reach more than 50% to third generation male sterility percentage 5. said process is repeated, forth generation reach 70% with
On, in the 5th generation, reached more than 80%, and the 6th generation, with maternal and 1 pair of 1 pollination of male parent, obtained male sterility percentage 100%, and Objective
The stable sunflower male sterile line of shape and maintainer.
Claims (4)
1. a kind of selection of edible oil certain herbaceous plants with big flowers male sterile line, it is characterised in that：Male sterile line is found in the colony of sunflower
Plant, gathers the plantation of its seed and obtains that multiple generation maternal plant baggings are standby, separately the sunflower colony with objective trait point
Into multiple generation male parent groups, group quantity is identical with the maternal strain number of the generation, plant bagging, each group group number, during pollination
The pollen of same male parent group is mixed, one plant of male sterile plants is authorized；Again each generation male sterility maternal plant
Seed is planted respectively obtains multiple two godmother this group for observation, while the seed for gathering each generation male parent group is planted respectively
Plant two godfather this group obtained as much, plant bagging, group numbering；Two godmother's in-groups are observed, male sterility percentage is chosen
Multiple male sterility maternal plants in one group of highest, while two generation plant point of previous generation's male parent group this group
For multiple male parent groups, each group gathers the maternal individual plant of one plant of male sterility that selected group is authorized after each strain pollen mixing；
Repeat the mode of the previous generation, until male sterility percentage reaches 80%, then with selected 1 pair of 1 pollination of male parent, until obtaining male sterility
Rate 100%, and the male sterile line with stable objects character.
2. a kind of selection of edible oil certain herbaceous plants with big flowers male sterile line according to claim 1, it is characterised in that：The male is not
It is that plant is selected from crop field food certain herbaceous plants with big flowers to educate, and the maintainer male parent group is " enjoying standing grain wide-margin-white " sunflower.
3. a kind of selection of edible oil certain herbaceous plants with big flowers male sterile line according to claim 2, it is characterised in that：It is described " to enjoy
Standing grain wide-margin-white " is that the detailed process that male parent cultivates male sterile line is：
（1）Male sterile line individual plant is found in sunflower field, selection ripe seed plantation obtains A plants of male sterile plants and is
A generation is maternal, numbering bagging, and in " enjoying standing grain wide-margin-white " the earth the inside, preferably AxB plants are generation male parent, is divided into A groups, every group B plants,
Numbering, bagging, during pollination, to one plant of maternal pollination after the pollen mixing of B plants of collections of every group of male parent；
（2）The seed of A plants of generation maternal plants is planted respectively obtains A groups two generations maternal plant, while the godfather of plantation A groups one respectively
This seed obtains two generation paternal plants, observes maternal plant developmental state, takes male sterility percentage highest suite number bagging,
Other each groups are abandoned, and from this one group of related paternal plant numbering bagging to selected two godmother, other each groups are abandoned, selected
C plants of male sterile plants of selection are standby in two generation maternal plants, and selected two generations paternal plant is divided into C groups, numbers respectively, pollination
When, paternal pollen is collected in units of group, one plant of female parent is authorized after every group of pollen mixing；
（3）The seed of C strain two generations maternal plants is planted respectively obtains C group three generations maternal plants, while the godfather of plantation C groups two respectively
This seed obtains C group three generations's paternal plants, and the maternal developmental state of observation takes male sterility percentage highest suite number bagging,
Other each groups are abandoned, from the one group paternal plant numbering bagging related to selected three generations female parent, and other each group male parents are abandoned,
D plants of male sterile plants of selection are standby in selected three generations maternal plant, and selected three generations's paternal plant is divided into D groups, numbers, awards respectively
In powder season, each strain pollen is collected in units of group, one plant of female parent is authorized after every group of pollen mixing；
（4）The seed of D plants of three generations maternal plants is planted respectively obtains D groups four generations maternal plant, while the godfather of plantation D groups three respectively
This seed obtains D groups four generations paternal plant, and the maternal developmental state of observation takes male sterility percentage highest suite number bagging,
Other each group female parents are abandoned, and from this one group of related paternal plant numbering bagging to selected four godmother, other each group male parents are put
Abandon, E plants of male sterile plants of selection are standby in selected four generations maternal plant, and selected four generations paternal plant is divided into E groups, compiles respectively
Number, during pollination, each strain pollen is collected in units of group, one plant of female parent is authorized after every group of pollen mixing；
（5）Said process is repeated, and is pollinated for 1 pair 1 when acquisition male sterility percentage is sufficiently large, then with maternal and male parent, directly
To male sterility percentage 100%, and during objective trait stabilization, obtain being available for the sunflower male sterile line and maintainer of crossbreeding.
4. a kind of selection of edible oil certain herbaceous plants with big flowers male sterile line according to claim 2, it is characterised in that：It is described " to enjoy
Standing grain wide-margin-white " is that the detailed process that male parent cultivates male sterile line is：
（1）Male sterile line individual plant is found in food certain herbaceous plants with big flowers field, selection ripe seed plantation obtains 10 plants of male sterile plants and is
A generation is maternal, numbering bagging, and in " enjoying standing grain wide-margin-white " the earth the inside, preferably 500 plants are generation male parent, are divided into 10 groups, every group 50
Strain, numbering, bagging, during pollination, to one plant of maternal pollination after the pollen mixing of 50 plants of collections of every group of male parent；
（2）The seed of 10 plants of generation maternal plants is planted respectively obtains 10 group of two generation plant, the 10 groups of generation male parents planted respectively
Seed obtain 10 group of two generation paternal plant, the maternal developmental state of observation takes male sterility percentage highest suite number bagging, its
He abandons each group, to this related suite number bagging of selected two godmother, other each groups are abandoned, in this plant of selected two godmother
10 plants of male sterile plants are chosen in strain, selected 500 plants of two generations paternal plant is divided into 10 groups, numbered respectively, pollinated season, collected
Each strain pollen, is mixed in units of group, and every group of pollen authorizes one plant of female parent；
（3）The seed of 10 plant of two generation maternal plant is planted respectively obtains 10 groups of three generations maternal plants, and 10 group of two godfather is planted respectively
This seed obtains 10 groups of three generations's paternal plants, observes developmental state, takes male sterility percentage highest suite number bagging, other
Each group female parent is abandoned, and from the one group paternal plant numbering bagging related to selected three generations female parent, other each groups are abandoned, selected
10 plants of male sterile plants are chosen in three generations maternal plant, selected 500 plants of three generations's paternal plant is divided into 10 groups, numbers, award respectively
During powder, each strain pollen is collected, is mixed in units of group, every group of pollen authorizes one plant of female parent；
（4）The seed of 10 plants of three generations maternal plants is planted respectively obtains 10 group of four generation maternal plant, and 10 group of three godfather is planted respectively
This seed obtains four generation paternal plants, and the maternal developmental state of observation takes male sterility percentage highest suite number bagging, other
Each group is abandoned, to this related suite number bagging of selected four godmother, other each groups are abandoned, in selected four generations maternal plant
10 plants of male sterile plants of middle selection, selected 500 plants of four generations paternal plant is divided into 10 groups, numbered respectively, during pollination, collects each strain
Pollen, is mixed in units of group, and every group of pollen authorizes one plant of female parent；
（5）Said process is repeated, when reaching more than 80% to male sterility percentage, then with maternal and 1 pair of 1 pollination of male parent, to hero
Property sterile rate 100%, and objective trait it is stable when, obtain sunflower male sterile line and maintainer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710390335.2A CN107079809B (en) | 2017-05-27 | 2017-05-27 | A kind of selection of edible oil certain herbaceous plants with big flowers male sterile line |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710390335.2A CN107079809B (en) | 2017-05-27 | 2017-05-27 | A kind of selection of edible oil certain herbaceous plants with big flowers male sterile line |
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| Publication Number | Publication Date |
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| CN107079809A true CN107079809A (en) | 2017-08-22 |
| CN107079809B CN107079809B (en) | 2019-03-19 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104642096A (en) * | 2015-02-17 | 2015-05-27 | 新疆康地种业科技股份有限公司 | Breeding method for novel 19# sterile line 1193A of oil sunflower |
| CN104969769A (en) * | 2015-08-06 | 2015-10-14 | 济南邦地生物工程有限公司 | Hybrid seed production method of sterile line of Abelmoschus esculentus Linn. |
| CN105340725A (en) * | 2015-11-13 | 2016-02-24 | 山西省农业科学院小麦研究所 | Edible sunflower breeding method of sterile, maintainer and restorer lines |
| CN106613892A (en) * | 2015-08-07 | 2017-05-10 | 民勤县全盛永泰农业有限公司 | Novel edible sunflower seed breeding method |
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| CN104642096A (en) * | 2015-02-17 | 2015-05-27 | 新疆康地种业科技股份有限公司 | Breeding method for novel 19# sterile line 1193A of oil sunflower |
| CN104969769A (en) * | 2015-08-06 | 2015-10-14 | 济南邦地生物工程有限公司 | Hybrid seed production method of sterile line of Abelmoschus esculentus Linn. |
| CN106613892A (en) * | 2015-08-07 | 2017-05-10 | 民勤县全盛永泰农业有限公司 | Novel edible sunflower seed breeding method |
| CN105340725A (en) * | 2015-11-13 | 2016-02-24 | 山西省农业科学院小麦研究所 | Edible sunflower breeding method of sterile, maintainer and restorer lines |
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| 刘平等: "《植物新品种保护通论》", 30 June 2011 * |
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