CN107075585B - 具有锁核酸的序列转换和信号扩增dna以及使用其的检测方法 - Google Patents

具有锁核酸的序列转换和信号扩增dna以及使用其的检测方法 Download PDF

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Publication number
CN107075585B
CN107075585B CN201580056020.3A CN201580056020A CN107075585B CN 107075585 B CN107075585 B CN 107075585B CN 201580056020 A CN201580056020 A CN 201580056020A CN 107075585 B CN107075585 B CN 107075585B
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nucleic acid
dna
sequence
oligonucleotide
locked nucleic
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CN107075585A8 (zh
CN107075585A (zh
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小宫健
小森诚
吉村徹
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Tokyo Institute of Technology NUC
Abbott Laboratories
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Abbott Laboratories
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Biomedical Technology (AREA)
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CN201580056020.3A 2014-10-14 2015-10-13 具有锁核酸的序列转换和信号扩增dna以及使用其的检测方法 Active CN107075585B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201462063666P 2014-10-14 2014-10-14
US62/063,666 2014-10-14
US201462098066P 2014-12-30 2014-12-30
US62/098,066 2014-12-30
PCT/IB2015/002141 WO2016059473A2 (en) 2014-10-14 2015-10-13 Sequence conversion and signal amplifier dna having locked nucleic acids and detection methods using same

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CN107075585A CN107075585A (zh) 2017-08-18
CN107075585A8 CN107075585A8 (zh) 2019-02-15
CN107075585B true CN107075585B (zh) 2021-07-20

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CN201580056019.0A Active CN107109473B (zh) 2014-10-14 2015-10-13 具有多dna间隔序列的序列转换和信号扩增dna及使用其的检测方法

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US (2) US10208333B2 (enExample)
EP (2) EP3207160B1 (enExample)
JP (2) JP6896620B2 (enExample)
CN (2) CN107075585B (enExample)
ES (2) ES2759337T3 (enExample)
WO (2) WO2016059474A2 (enExample)

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* Cited by examiner, † Cited by third party
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WO2012077819A1 (ja) 2010-12-10 2012-06-14 国立大学法人 東京工業大学 標的核酸の検出方法及びキット
EP3094753B1 (en) 2014-01-15 2019-10-16 Abbott Laboratories Covered sequence conversion dna and detection methods
EP3207160B1 (en) 2014-10-14 2019-11-20 Abbott Laboratories Sequence conversion and signal amplifier dna having locked nucleic acids and detection methods using same
US10604790B2 (en) 2014-12-24 2020-03-31 Abbott Laboratories Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
WO2021252733A2 (en) * 2020-06-10 2021-12-16 Bonadea Diagnostics, Llc Sequence conversion reaction
WO2023107713A2 (en) * 2021-12-09 2023-06-15 Bonadea Diagnostics, Llc Sequence conversion reaction

Citations (1)

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CN1748028A (zh) * 2003-02-21 2006-03-15 卫材株式会社 用于变异基因检测的信号扩增方法

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CN102399871B (zh) * 2011-10-24 2014-04-16 中国科学院广州生物医药与健康研究院 耐热等温核酸检测试剂及其试剂盒和检测方法
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EP2722399A1 (en) * 2012-10-18 2014-04-23 Roche Diagniostics GmbH Method for preventing high molecular weight products during amplification
EP3094753B1 (en) 2014-01-15 2019-10-16 Abbott Laboratories Covered sequence conversion dna and detection methods
CN103789435B (zh) 2014-01-29 2015-08-26 西安交通大学 一种基于级联恒温扩增的miRNA荧光检测试剂盒及方法
EP3207160B1 (en) 2014-10-14 2019-11-20 Abbott Laboratories Sequence conversion and signal amplifier dna having locked nucleic acids and detection methods using same

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Publication number Publication date
EP3207159B1 (en) 2019-11-20
CN107075585A8 (zh) 2019-02-15
US10208333B2 (en) 2019-02-19
US20160102339A1 (en) 2016-04-14
US20160102345A1 (en) 2016-04-14
JP2017530715A (ja) 2017-10-19
EP3207160B1 (en) 2019-11-20
JP2017532044A (ja) 2017-11-02
JP6896620B2 (ja) 2021-06-30
EP3207159A2 (en) 2017-08-23
WO2016059474A3 (en) 2016-06-09
ES2759337T3 (es) 2020-05-08
EP3207160A4 (en) 2018-05-23
JP6773651B2 (ja) 2020-10-21
WO2016059473A2 (en) 2016-04-21
CN107109473B (zh) 2020-11-06
WO2016059473A3 (en) 2016-06-23
WO2016059474A2 (en) 2016-04-21
US10316353B2 (en) 2019-06-11
EP3207159A4 (en) 2018-05-30
CN107109473A (zh) 2017-08-29
ES2759339T3 (es) 2020-05-08
EP3207160A2 (en) 2017-08-23
CN107075585A (zh) 2017-08-18

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