CN107075566B - 用于制备核酸的等温方法及相关组合物 - Google Patents

用于制备核酸的等温方法及相关组合物 Download PDF

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Publication number
CN107075566B
CN107075566B CN201580016111.4A CN201580016111A CN107075566B CN 107075566 B CN107075566 B CN 107075566B CN 201580016111 A CN201580016111 A CN 201580016111A CN 107075566 B CN107075566 B CN 107075566B
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nucleic acid
rna
template
sequence
reaction
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Chinese (zh)
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CN107075566A (zh
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乔舒阿·斯塔尔
贾森·迈尔斯
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Asherdex Co ltd
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Asherdex Co ltd
ArcherDx LLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201580016111.4A 2014-01-27 2015-01-26 用于制备核酸的等温方法及相关组合物 Expired - Fee Related CN107075566B (zh)

Priority Applications (1)

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CN202110571327.4A CN113388664A (zh) 2014-01-27 2015-01-26 用于制备核酸的等温方法及相关组合物

Applications Claiming Priority (3)

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US201461931974P 2014-01-27 2014-01-27
US61/931,974 2014-01-27
PCT/US2015/012842 WO2015112949A2 (en) 2014-01-27 2015-01-26 Isothermal methods and related compositions for preparing nucleic acids

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CN107075566A CN107075566A (zh) 2017-08-18
CN107075566B true CN107075566B (zh) 2021-06-15

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US (1) US20150337364A1 (enExample)
EP (1) EP3099819A4 (enExample)
JP (2) JP2017504346A (enExample)
CN (2) CN107075566B (enExample)
AU (2) AU2015209103B2 (enExample)
CA (1) CA2938141A1 (enExample)
WO (1) WO2015112949A2 (enExample)

Families Citing this family (9)

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Publication number Priority date Publication date Assignee Title
CN106192022B (zh) * 2016-08-08 2018-07-03 中国科学院北京基因组研究所 16SrRNA多重测序文库的构建方法
CN106191045B (zh) * 2016-08-08 2019-10-11 中国科学院北京基因组研究所 用于多重核酸测序的Index和引物
JP6997772B2 (ja) 2016-09-15 2022-01-18 アーチャーディーエックス， エルエルシー 核酸サンプル調製の方法
EP4353830A3 (en) 2016-09-15 2024-07-03 ArcherDX, LLC Methods of nucleic acid sample preparation for analysis of cell-free dna
CN110312825A (zh) 2016-10-24 2019-10-08 吉内恩福赛克公司 隐藏核酸内存在的信息
KR102103719B1 (ko) * 2018-05-18 2020-04-23 주식회사 바이나리 등온핵산 증폭을 이용한 생체조직의 3차원 핵산영상 분석 방법
CN111378724B (zh) * 2018-12-27 2024-03-22 上海仁度生物科技股份有限公司 一种rna放大检测方法及检测试剂盒
GB201903391D0 (en) * 2019-03-12 2019-04-24 Cancer Research Tech Ltd Nucleic acid amplification methods
GB201911515D0 (en) * 2019-08-12 2019-09-25 Univ London Queen Mary Methods for generating a population of polynucleotide molecules

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010077288A2 (en) * 2008-12-09 2010-07-08 The Salk Institute For Biological Studies Methods for identifying differences in alternative splicing between two rna samples
US7972820B2 (en) * 2000-12-08 2011-07-05 Illumina Cambridge Limited Isothermal amplification of nucleic acids on a solid support

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CA2020958C (en) * 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
EP0731174B1 (en) * 1989-07-11 2004-11-17 Gen-Probe Incorporated Nucleic acid sequence amplification methods
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
WO1998021361A1 (en) * 1996-11-12 1998-05-22 Visible Genetics Inc. Method and kit for direct isothermal sequencing of nucleic acids
US6197554B1 (en) * 1998-11-20 2001-03-06 Shi-Lung Lin Method for generating full-length cDNA library from single cells
US20030022318A1 (en) * 2000-01-25 2003-01-30 Epiclone, Inc. Method for thermocycling amplification of nucleic acid sequences and the generation of related peptides thereof
US20030104432A1 (en) * 2001-07-27 2003-06-05 The Regents Of The University Of California Methods of amplifying sense strand RNA
WO2009091719A1 (en) * 2008-01-14 2009-07-23 Applera Corporation Compositions, methods, and kits for detecting ribonucleic acid
CN101270395A (zh) * 2008-05-08 2008-09-24 重庆大学 柑桔衰退病毒一步法实时荧光反转录聚合酶链式反应固相化试剂盒及其检测方法
JP2010143860A (ja) * 2008-12-19 2010-07-01 Chisso Corp タンパク質の安定化剤
CN102791884A (zh) * 2010-01-08 2012-11-21 奇亚根盖瑟斯堡股份有限公司 用于等温核酸扩增的材料和方法
GB201008125D0 (en) * 2010-05-14 2010-06-30 Biofortuna Ltd Tissue typing assays and kits
US20160108468A1 (en) * 2013-03-06 2016-04-21 Ohio State Innovation Foundation Isothermal Amplification of Nucleic Acid, and Library Preparation and Clone Generation in Sequencing

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7972820B2 (en) * 2000-12-08 2011-07-05 Illumina Cambridge Limited Isothermal amplification of nucleic acids on a solid support
WO2010077288A2 (en) * 2008-12-09 2010-07-08 The Salk Institute For Biological Studies Methods for identifying differences in alternative splicing between two rna samples

Also Published As

Publication number Publication date
AU2021240263A1 (en) 2021-10-28
EP3099819A2 (en) 2016-12-07
CA2938141A1 (en) 2015-07-30
AU2015209103A1 (en) 2016-09-15
EP3099819A4 (en) 2018-01-10
JP2021094038A (ja) 2021-06-24
WO2015112949A3 (en) 2015-10-22
US20150337364A1 (en) 2015-11-26
JP2017504346A (ja) 2017-02-09
WO2015112949A2 (en) 2015-07-30
AU2015209103B2 (en) 2021-07-01
CN113388664A (zh) 2021-09-14
CN107075566A (zh) 2017-08-18

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