AU2015209103B2 - Isothermal methods and related compositions for preparing nucleic acids - Google Patents

Isothermal methods and related compositions for preparing nucleic acids Download PDF

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Publication number
AU2015209103B2
AU2015209103B2 AU2015209103A AU2015209103A AU2015209103B2 AU 2015209103 B2 AU2015209103 B2 AU 2015209103B2 AU 2015209103 A AU2015209103 A AU 2015209103A AU 2015209103 A AU2015209103 A AU 2015209103A AU 2015209103 B2 AU2015209103 B2 AU 2015209103B2
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AU
Australia
Prior art keywords
rna
sequence
dna
nucleic acid
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2015209103A
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English (en)
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AU2015209103A1 (en
Inventor
Jason Myers
Joshua STAHL
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ArcherDx LLC
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ArcherDx LLC
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Publication date
Application filed by ArcherDx LLC filed Critical ArcherDx LLC
Publication of AU2015209103A1 publication Critical patent/AU2015209103A1/en
Application granted granted Critical
Publication of AU2015209103B2 publication Critical patent/AU2015209103B2/en
Assigned to ARCHERDX, LLC reassignment ARCHERDX, LLC Request for Assignment Assignors: ArcherDX, Inc.
Priority to AU2021240263A priority Critical patent/AU2021240263A1/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2015209103A 2014-01-27 2015-01-26 Isothermal methods and related compositions for preparing nucleic acids Ceased AU2015209103B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2021240263A AU2021240263A1 (en) 2014-01-27 2021-09-30 Isothermal methods and related compositions for preparing nucleic acids

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461931974P 2014-01-27 2014-01-27
US61/931,974 2014-01-27
PCT/US2015/012842 WO2015112949A2 (en) 2014-01-27 2015-01-26 Isothermal methods and related compositions for preparing nucleic acids

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2021240263A Division AU2021240263A1 (en) 2014-01-27 2021-09-30 Isothermal methods and related compositions for preparing nucleic acids

Publications (2)

Publication Number Publication Date
AU2015209103A1 AU2015209103A1 (en) 2016-09-15
AU2015209103B2 true AU2015209103B2 (en) 2021-07-01

Family

ID=53682134

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2015209103A Ceased AU2015209103B2 (en) 2014-01-27 2015-01-26 Isothermal methods and related compositions for preparing nucleic acids
AU2021240263A Withdrawn AU2021240263A1 (en) 2014-01-27 2021-09-30 Isothermal methods and related compositions for preparing nucleic acids

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2021240263A Withdrawn AU2021240263A1 (en) 2014-01-27 2021-09-30 Isothermal methods and related compositions for preparing nucleic acids

Country Status (7)

Country Link
US (1) US20150337364A1 (enExample)
EP (1) EP3099819A4 (enExample)
JP (2) JP2017504346A (enExample)
CN (2) CN113388664A (enExample)
AU (2) AU2015209103B2 (enExample)
CA (1) CA2938141A1 (enExample)
WO (1) WO2015112949A2 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106192022B (zh) * 2016-08-08 2018-07-03 中国科学院北京基因组研究所 16SrRNA多重测序文库的构建方法
CN106191045B (zh) * 2016-08-08 2019-10-11 中国科学院北京基因组研究所 用于多重核酸测序的Index和引物
CA3037185A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation
US10683531B2 (en) 2016-09-15 2020-06-16 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free DNA
WO2018081113A1 (en) 2016-10-24 2018-05-03 Sawaya Sterling Concealing information present within nucleic acids
KR102103719B1 (ko) * 2018-05-18 2020-04-23 주식회사 바이나리 등온핵산 증폭을 이용한 생체조직의 3차원 핵산영상 분석 방법
CN111378724B (zh) * 2018-12-27 2024-03-22 上海仁度生物科技股份有限公司 一种rna放大检测方法及检测试剂盒
GB201903391D0 (en) * 2019-03-12 2019-04-24 Cancer Research Tech Ltd Nucleic acid amplification methods
GB201911515D0 (en) * 2019-08-12 2019-09-25 Univ London Queen Mary Methods for generating a population of polynucleotide molecules

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824518A (en) * 1989-07-11 1998-10-20 Gen-Probe Incorporated Nucleic acid sequence amplification methods
US6197554B1 (en) * 1998-11-20 2001-03-06 Shi-Lung Lin Method for generating full-length cDNA library from single cells
US20030022318A1 (en) * 2000-01-25 2003-01-30 Epiclone, Inc. Method for thermocycling amplification of nucleic acid sequences and the generation of related peptides thereof
US20100279305A1 (en) * 2008-01-14 2010-11-04 Applied Biosystems, Llc Compositions, methods, and kits for detecting ribonucleic acid

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0731175A3 (en) * 1989-07-11 2004-05-26 Gen-Probe Incorporated Oligonucleotide probes for HIV nucleic acid
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
AU4938497A (en) * 1996-11-12 1998-06-03 Visible Genetics Inc. Method and kit for direct isothermal sequencing of nucleic acids
AR031640A1 (es) * 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US20030104432A1 (en) * 2001-07-27 2003-06-05 The Regents Of The University Of California Methods of amplifying sense strand RNA
CN101270395A (zh) * 2008-05-08 2008-09-24 重庆大学 柑桔衰退病毒一步法实时荧光反转录聚合酶链式反应固相化试剂盒及其检测方法
WO2010077288A2 (en) * 2008-12-09 2010-07-08 The Salk Institute For Biological Studies Methods for identifying differences in alternative splicing between two rna samples
JP2010143860A (ja) * 2008-12-19 2010-07-01 Chisso Corp タンパク質の安定化剤
CA2786473A1 (en) * 2010-01-08 2011-07-14 Qiagen Gaithersburg, Inc. Materials and methods for isothermal nucleic acid amplification
GB201008125D0 (en) * 2010-05-14 2010-06-30 Biofortuna Ltd Tissue typing assays and kits
WO2014138385A1 (en) * 2013-03-06 2014-09-12 Ohio State Innovation Foundation Isothermal amplification of nuleic acid, and library preparation and clone generation in sequencing

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824518A (en) * 1989-07-11 1998-10-20 Gen-Probe Incorporated Nucleic acid sequence amplification methods
US6197554B1 (en) * 1998-11-20 2001-03-06 Shi-Lung Lin Method for generating full-length cDNA library from single cells
US20030022318A1 (en) * 2000-01-25 2003-01-30 Epiclone, Inc. Method for thermocycling amplification of nucleic acid sequences and the generation of related peptides thereof
US20100279305A1 (en) * 2008-01-14 2010-11-04 Applied Biosystems, Llc Compositions, methods, and kits for detecting ribonucleic acid

Also Published As

Publication number Publication date
US20150337364A1 (en) 2015-11-26
WO2015112949A3 (en) 2015-10-22
AU2015209103A1 (en) 2016-09-15
JP2017504346A (ja) 2017-02-09
CN107075566B (zh) 2021-06-15
WO2015112949A2 (en) 2015-07-30
CN107075566A (zh) 2017-08-18
EP3099819A2 (en) 2016-12-07
AU2021240263A1 (en) 2021-10-28
CN113388664A (zh) 2021-09-14
CA2938141A1 (en) 2015-07-30
JP2021094038A (ja) 2021-06-24
EP3099819A4 (en) 2018-01-10

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PC1 Assignment before grant (sect. 113)

Owner name: ARCHERDX, LLC

Free format text: FORMER APPLICANT(S): ARCHERDX, INC.

FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired