CN107058490A - 肠道及口腔菌群多样性及差异性的分析方法 - Google Patents
肠道及口腔菌群多样性及差异性的分析方法 Download PDFInfo
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Abstract
Description
试剂 | 使用量 | 终浓度 |
Taq酶溶液 | 0.8微升 | |
上游引物(10微摩) | 0.5微升 | 0.1微摩 |
下游引物(10微摩) | 0.5微升 | 0.1微摩 |
10×buffer溶液 | 5微升 | |
DNA模板 | 2微升 | |
dNTP溶液 | 4微升 | |
灭菌蒸馏水 | 37.2微升 | |
总体积 | 15微升 |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107475432A (zh) * | 2017-09-30 | 2017-12-15 | 上海交通大学医学院附属瑞金医院 | 一种肠道菌群多样性的检测方法 |
CN108486271A (zh) * | 2018-03-29 | 2018-09-04 | 中国科学院微生物研究所 | 基于高通量测序技术的银耳菌种纯度检测方法及其应用 |
CN109266733A (zh) * | 2018-09-25 | 2019-01-25 | 深圳市英马诺生物科技有限公司 | 自闭症肠道菌群毒力因子基因及其应用 |
CN109706235A (zh) * | 2019-01-29 | 2019-05-03 | 广州康昕瑞基因健康科技有限公司 | 一种肠道微生物菌群的检测和分析方法及其系统 |
WO2019114049A1 (zh) | 2017-12-12 | 2019-06-20 | 苏州普瑞森基因科技有限公司 | 一种用于分析肠道微生物的引物组合物及其应用 |
CN110241190A (zh) * | 2019-06-24 | 2019-09-17 | 东华大学 | 一种获得小鼠死亡时间间隔的方法 |
CN111394485A (zh) * | 2020-03-30 | 2020-07-10 | 云南中烟工业有限责任公司 | 一种基于唾液微生物检测人是否吸烟的引物对、试剂盒及方法 |
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CN105603066A (zh) * | 2016-01-13 | 2016-05-25 | 金锋 | 精神障碍的肠道微生物标志物及其应用 |
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CN105979952A (zh) * | 2013-11-25 | 2016-09-28 | 赛里斯治疗公司 | 协同细菌组合物以及其制造方法和用途 |
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Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107475432A (zh) * | 2017-09-30 | 2017-12-15 | 上海交通大学医学院附属瑞金医院 | 一种肠道菌群多样性的检测方法 |
WO2019114049A1 (zh) | 2017-12-12 | 2019-06-20 | 苏州普瑞森基因科技有限公司 | 一种用于分析肠道微生物的引物组合物及其应用 |
US11401561B2 (en) | 2017-12-12 | 2022-08-02 | Suzhou Precision Gene Co. Ltd. | Primer composition for analyzing intestinal flora and application thereof |
CN108486271A (zh) * | 2018-03-29 | 2018-09-04 | 中国科学院微生物研究所 | 基于高通量测序技术的银耳菌种纯度检测方法及其应用 |
CN108486271B (zh) * | 2018-03-29 | 2019-03-29 | 中国科学院微生物研究所 | 基于高通量测序技术的银耳菌种纯度检测方法及其应用 |
CN109266733A (zh) * | 2018-09-25 | 2019-01-25 | 深圳市英马诺生物科技有限公司 | 自闭症肠道菌群毒力因子基因及其应用 |
CN109266733B (zh) * | 2018-09-25 | 2022-03-04 | 深圳市英马诺生物科技有限公司 | 自闭症肠道菌群毒力因子基因及其应用 |
CN109706235A (zh) * | 2019-01-29 | 2019-05-03 | 广州康昕瑞基因健康科技有限公司 | 一种肠道微生物菌群的检测和分析方法及其系统 |
CN110241190A (zh) * | 2019-06-24 | 2019-09-17 | 东华大学 | 一种获得小鼠死亡时间间隔的方法 |
CN110241190B (zh) * | 2019-06-24 | 2023-05-02 | 东华大学 | 一种获得小鼠死亡时间间隔的方法 |
CN111394485A (zh) * | 2020-03-30 | 2020-07-10 | 云南中烟工业有限责任公司 | 一种基于唾液微生物检测人是否吸烟的引物对、试剂盒及方法 |
CN111394485B (zh) * | 2020-03-30 | 2023-09-22 | 云南中烟工业有限责任公司 | 一种基于唾液微生物检测人是否吸烟的引物对、试剂盒及方法 |
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