CN107050164A - A kind of antibacterial extract extracting method of longan seed - Google Patents
A kind of antibacterial extract extracting method of longan seed Download PDFInfo
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- CN107050164A CN107050164A CN201710110157.3A CN201710110157A CN107050164A CN 107050164 A CN107050164 A CN 107050164A CN 201710110157 A CN201710110157 A CN 201710110157A CN 107050164 A CN107050164 A CN 107050164A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/77—Sapindaceae (Soapberry family), e.g. lychee or soapberry
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The present invention relates to extractive technique field, more particularly, to a kind of antibacterial extract extracting method of longan seed, comprise the following steps：(1) longan seed cleans drying；(2) longan seed after drying is crushed, obtains longan seed powder；(3) longan seed powder is added water and sized mixing, then added alpha-amylase and digested, obtain primary zymolyte；(4) primary zymolyte addition cellulase is digested into (5) zymolyte and uses carbon dioxide supercritical extraction；(6) extract adds 45 60% ethanol heating extractions and obtains leachate；(7) leachate is filtered under diminished pressure drying, obtains longan seed antipathogenic composition crude extract；(8) longan seed antipathogenic composition crude extract is extracted with 40 45% acetone soln, filtering is flowed through macroreticular resin and adsorbed；(9) by the resin filter after absorption, eluted with 40% ethanol, dried after eluent concentration, obtain antipathogenic composition extract.The present invention can effectively extract longan seed antipathogenic composition.
Description
【Technical field】
The present invention relates to polyphenol extractive technique field, more particularly, to a kind of antibacterial extract extracting method of longan seed.
【Background technology】
Longan, is the fruit of sapindaceous plant longan, and warm-natured sweet, heart-spleen boosting fills blood, and has nourishing to mend with good
Benefit effect, available for diseases such as the insomnia forgetfulness palpitation with fear dizziness caused by heart and spleen deficiency insufficiency of vital energy and blood, China is big for world's YEAST IN LONGAN PRODUCTION
State, main product exceedes in ground cultivated areas such as Guangdong, Guangxi Fujian Sichuan, and longan meat production is more than ten thousand tons.Longan seed is longan
Benevolence, diameter 0.8-1.2cm, epidermis bronzing is glossy, and there is oblong canescence hilum one end, and matter is hard.Smell is micro- light
And slight bitter.The quality of longan seed accounts for 17% or so of longan fruit quality.Comprehensively utilized due to no, every year discarded dragon
Eye nuclear mass reaches hundreds of thousands ton.
Longan is in addition to longan pulp has very high nutritive value, and its seed also has remarkable efficacy.Longan seed contains rich
The natural products such as rich starch, reduced sugar, crude fibre, protein, pectin, polyphenol, while also containing several kinds of mineral elements, dimension life
The nutrient such as element and the necessary amino acid of human body.Wherein content of starch is 60.88%, and content of reducing sugar is 9.67%, crude fibre
Content is 6.85%, and protein content is 5.58%, and pectin content is 5.19%, and polyphenol content is 4.72%, and fat content is
3.23%.And polyphenol therein is otherwise known as " the 7th class nutrient ", polyphenol is polyhydroxy benzenes, such as benzenediol, benzenetriol, is
The phenols secondary metabolite produced in plant and microorganism, with anti-oxidant, anticoagulation, with the spy such as the complexing of metal ion
Property.Polyphenol, with functions such as anti-aging, radioresistance, clear body free radicals, is prevented cardiovascular and cerebrovascular disease as natural
Control, there are good effects in terms of such as coronary heart disease, atherosclerosis, cerebral thrombus.To the discarded object longan seed of longan deep processing
Exploitation, it has also become the food in the main place of production of the longan such as Guangdong and Guangxi Provinces, Fujian, one of processing of farm products direction research in recent years
Focus.And studied at this stage for many phenol extractions of longan seed and purifying process, have not yet to see the technique with actual production meaning
The report of method, this is intended merely to carry out the structure of matter and bioactivity mainly due to most of its purpose of research at present
Analysis, the scale still only rests on laboratory stage, and equipment investment, production cost are higher, complex process, and introducing adds
Plus, impurity component it is more, the purity of finished product and activity also need further raising.
【The content of the invention】
In view of the foregoing, it is necessary to provide a kind of longan seed antibacterial extract extracting method, to make full use of longan seed
Healthy nutritive value.
The purpose of the present invention is achieved through the following technical solutions：
A kind of antibacterial extract extracting method of longan seed, it is characterised in that comprise the following steps：
(1) longan seed cleans drying；
(2) longan seed after drying is crushed, obtains longan seed powder；
(3) longan seed powder is added water and sized mixing, then added alpha-amylase and digested, obtain primary zymolyte；
(4) primary zymolyte addition cellulase is digested, obtains zymolyte；
(5) zymolyte uses carbon dioxide supercritical extraction；
(6) extract adds 45-60% ethanol heating extractions and obtains leachate；
(7) leachate is filtered under diminished pressure drying, obtains longan seed antipathogenic composition crude extract；
(8) longan seed antipathogenic composition crude extract is extracted with 40-45% acetone soln, filtering is flowed through macroreticular resin and entered
Row absorption；
(9) by the resin filter after absorption, eluted with 40% ethanol, eluent concentration after dry, obtain it is antibacterial into
Divide extract.
Further, the longan seed drying makes the final percentage of water loss of longan seed reach 25%-32%, and the drying temperature is
45-55 DEG C, drying time is 15-30h.
Further, the longan seed is ground into 30-40 mesh.
Further, the step 3) in add water and size mixing by material-water ratio 1: 4, after stirring, every gram of material aqueous mixtures add
Enter 1200U alpha-amylases, liquefied 30-60 minutes at pH5.5,90 DEG C of temperature.
Further, the step 4) in add 1200U cellulases by the 0.3% of primary zymolyte, in pH4.5, temperature
3h is digested at 40 DEG C of degree.
Further, the step 5) in carbon dioxide supercritical extraction temperature be 35-40 DEG C, pressure is 20-28MP.
Further, the step 8) macroreticular resin type be d-101B.
The method that the present invention extracts polyphenol from longan seed, for the optimization test parameter obtained after test of many times, each
Step can effectively improve the recovery rate and purity of longan seed bacteriostatic activity thing.
The present invention rationally controls polyphenol oxidase activity in longan seed moisture, reduction longan seed first with drying technology,
The loss of polyphenol is reduced in next step enzymolysis process.Longan seed is digested respectively using amylase and cellulase, makes longan seed many
Phenol is more easy to separate out the extraction beneficial to next step polyphenol.Using acetone soln as leachate, longan seed can be more effectively extracted
Antibacterial effective active composition.
Verified by test of many times checking through many experiments, macroreticular resin type be d-101B adsorption rates and resolution factor compared with
Height, it is optimal for the antibacterial effective active composition assimilation effect of longan seed.Eluted using 40% ethanol, eluting rate is relatively up to arrived
93.3%.
【Embodiment】
Embodiment 1
A kind of antibacterial extract extracting method of longan seed, it is characterised in that comprise the following steps：
(1) longan seed is cleaned, and is carried out 15h drying in the case where temperature is 45 DEG C, is made the final percentage of water loss of longan seed up to 25%；
(2) longan seed after drying is ground into 30 mesh, obtains longan seed powder；
(3) add water and size mixing by material-water ratio 1: 4, after stirring, every gram of material aqueous mixtures add 1200U alpha-amylases,
Liquefied 30 minutes at pH5.5,90 DEG C of temperature, obtain primary zymolyte；
(4) primary zymolyte addition cellulase is digested, 1200U fibers is added by the 0.3% of primary zymolyte
Plain enzyme, digests 3h at pH4.5,40 DEG C of temperature, obtains zymolyte；
(5) it is 35 DEG C in temperature, pressure uses carbon dioxide supercritical extraction for zymolyte under 20MP；
(6) extract adds 45% ethanol heating extraction and obtains leachate；
(7) leachate is filtered under diminished pressure drying, obtains longan seed antipathogenic composition crude extract；
(8) longan seed antipathogenic composition crude extract is extracted with 40% acetone soln, filtering flows through type big for d-101B
Hole resin is adsorbed；
(9) by the resin filter after absorption, eluted with 40% ethanol, eluent concentration after dry, obtain it is antibacterial into
Divide extract.
Embodiment 2
A kind of antibacterial extract extracting method of longan seed, it is characterised in that comprise the following steps：
(1) longan seed is cleaned, and is carried out 30h drying in the case where temperature is 55 DEG C, is made the final percentage of water loss of longan seed up to 32%；
(2) longan seed after drying is ground into 40 mesh, obtains longan seed powder；
(3) add water and size mixing by material-water ratio 1: 4, after stirring, every gram of material aqueous mixtures add 1200U alpha-amylases,
Liquefied 60 minutes at pH5.5,90 DEG C of temperature, obtain primary zymolyte；
(4) primary zymolyte addition cellulase is digested, 1200U fibers is added by the 0.3% of primary zymolyte
Plain enzyme, digests 3h at pH4.5,40 DEG C of temperature, obtains zymolyte；
(5) it is 40 DEG C in temperature, pressure uses carbon dioxide supercritical extraction for zymolyte under 28MP；
(6) extract adds 60% ethanol heating extraction and obtains leachate；
(7) leachate is filtered under diminished pressure drying, obtains longan seed antipathogenic composition crude extract；
(8) longan seed antipathogenic composition crude extract is extracted with 45% acetone soln, filtering flows through type big for d-101B
Hole resin is adsorbed；
(9) by the resin filter after absorption, eluted with 40% ethanol, eluent concentration after dry, obtain it is antibacterial into
Divide extract.
Embodiment 3
A kind of antibacterial extract extracting method of longan seed, it is characterised in that comprise the following steps：
(1) longan seed is cleaned, and is carried out 18h drying in the case where temperature is 50 DEG C, is made the final percentage of water loss of longan seed up to 29%；
(2) longan seed after drying is ground into 35 mesh, obtains longan seed powder；
(3) add water and size mixing by material-water ratio 1: 4, after stirring, every gram of material aqueous mixtures add 1200U alpha-amylases,
Liquefied 38 minutes at pH5.5,90 DEG C of temperature, obtain primary zymolyte；
(4) primary zymolyte addition cellulase is digested, 1200U fibers is added by the 0.3% of primary zymolyte
Plain enzyme, digests 3h at pH4.5,40 DEG C of temperature, obtains zymolyte；
(5) it is 37 DEG C in temperature, pressure uses carbon dioxide supercritical extraction for zymolyte under 25MP；
(6) extract adds 50% ethanol heating extraction and obtains leachate；
(7) leachate is filtered under diminished pressure drying, obtains longan seed antipathogenic composition crude extract；
(8) longan seed antipathogenic composition crude extract is extracted with 42% acetone soln, filtering flows through type big for d-101B
Hole resin is adsorbed；
(9) by the resin filter after absorption, eluted with 40% ethanol, eluent concentration after dry, obtain it is antibacterial into
Divide extract.
Embodiment 4
A kind of antibacterial extract extracting method of longan seed, it is characterised in that comprise the following steps：
(1) longan seed is cleaned, and is carried out 22h drying in the case where temperature is 52 DEG C, is made the final percentage of water loss of longan seed up to 28%；
(2) longan seed after drying is ground into 36 mesh, obtains longan seed powder；
(3) add water and size mixing by material-water ratio 1: 4, after stirring, every gram of material aqueous mixtures add 1200U alpha-amylases,
Liquefied 55 minutes at pH5.5,90 DEG C of temperature, obtain primary zymolyte；
(4) primary zymolyte addition cellulase is digested, 1200U fibers is added by the 0.3% of primary zymolyte
Plain enzyme, digests 3h at pH4.5,40 DEG C of temperature, obtains zymolyte；
(5) it is 34 DEG C in temperature, pressure uses carbon dioxide supercritical extraction for zymolyte under 23MP；
(6) extract adds 49% ethanol heating extraction and obtains leachate；
(7) leachate is filtered under diminished pressure drying, obtains longan seed antipathogenic composition crude extract；
(8) longan seed antipathogenic composition crude extract is extracted with 42% acetone soln, filtering flows through type big for d-101B
Hole resin is adsorbed；
(9) by the resin filter after absorption, eluted with 40% ethanol, eluent concentration after dry, obtain it is antibacterial into
Divide extract.
The foregoing is only a preferred embodiment of the present invention, therefore can not limit the scope that the present invention is implemented according to this, i.e.,
The equivalent changes and modifications made according to the scope of the claims of the present invention and description, all should still belong in the range of the present invention covers.
Claims (7)
1. a kind of antibacterial extract extracting method of longan seed, it is characterised in that comprise the following steps：
(1) longan seed cleans drying；
(2) longan seed after drying is crushed, obtains longan seed powder；
(3) longan seed powder is added water and sized mixing, then added alpha-amylase and digested, obtain primary zymolyte；
(4) primary zymolyte addition cellulase is digested, obtains zymolyte；
(5) zymolyte uses carbon dioxide supercritical extraction；
(6) extract adds 45-60% ethanol heating extractions and obtains leachate；
(7) leachate is filtered under diminished pressure drying, obtains longan seed antipathogenic composition crude extract；
(8) longan seed antipathogenic composition crude extract is extracted with 40-45% acetone soln, filtering is flowed through macroreticular resin and inhaled
It is attached；
(9) by the resin filter after absorption, eluted with 40% ethanol, dried after eluent concentration, obtain antipathogenic composition and carry
Take thing.
2. a kind of antibacterial extract extracting method of longan seed according to claim 1, it is characterised in that the longan seed drying
The final percentage of water loss of longan seed is set to reach 25%-32%, the drying temperature is 45-55 DEG C, and drying time is 15-30h.
3. the antibacterial extract extracting method of a kind of longan seed according to claim 1, it is characterised in that the longan seed is crushed
Into 30-40 mesh.
4. a kind of antibacterial extract extracting method of longan seed according to claim 1, it is characterised in that the step 3) in press
Material-water ratio 1: 4, which adds water, sizes mixing, after stirring, and every gram of material aqueous mixtures add 1200U alpha-amylases, in pH5.5, temperature 90
Liquefied 30-60 minutes at DEG C.
5. a kind of antibacterial extract extracting method of longan seed according to claim 1, it is characterised in that the step 4) in press
The 0.3% of primary zymolyte adds 1200U cellulases, and 3h is digested at pH4.5,40 DEG C of temperature.
6. a kind of antibacterial extract extracting method of longan seed according to claim 1, it is characterised in that the step 5) in two
The temperature of carbonoxide supercritical extract is 35-40 DEG C, and pressure is 20-28MP.
7. a kind of antibacterial extract extracting method of longan seed according to claim 1, it is characterised in that the step 8) it is big
Hole resinous type is d-101B.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710110157.3A CN107050164A (en) | 2017-02-28 | 2017-02-28 | A kind of antibacterial extract extracting method of longan seed |
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| CN201710110157.3A CN107050164A (en) | 2017-02-28 | 2017-02-28 | A kind of antibacterial extract extracting method of longan seed |
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| CN107050164A true CN107050164A (en) | 2017-08-18 |
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| CN201710110157.3A Pending CN107050164A (en) | 2017-02-28 | 2017-02-28 | A kind of antibacterial extract extracting method of longan seed |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104473825A (en) * | 2014-12-04 | 2015-04-01 | 广东食品药品职业学院 | Longan seed polyphenol extraction and purification method and application of longan seed polyphenol in preparing polyphenol whitening cosmetics |
-
2017
- 2017-02-28 CN CN201710110157.3A patent/CN107050164A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104473825A (en) * | 2014-12-04 | 2015-04-01 | 广东食品药品职业学院 | Longan seed polyphenol extraction and purification method and application of longan seed polyphenol in preparing polyphenol whitening cosmetics |
Non-Patent Citations (2)
| Title |
|---|
| 柯发敏等: "没食子酸的研究进展", 《泸州医学院学报》 * |
| 高文远: "《中药生物工程》", 31 January 2014, 上海科学技术出版社 * |
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