CN104473825A - Longan seed polyphenol extraction and purification method and application of longan seed polyphenol in preparing polyphenol whitening cosmetics - Google Patents
Longan seed polyphenol extraction and purification method and application of longan seed polyphenol in preparing polyphenol whitening cosmetics Download PDFInfo
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Abstract
The invention discloses a longan seed polyphenol extraction and purification method and application of the longan seed polyphenol in preparing polyphenol whitening cosmetics. The method comprises the following steps: respectively treating longan seed powder with anhydrous acetone and a 50-70% acetone water solution three times, merging the second and third filtrates, and recovering the acetone to obtain the polyphenol; and purifying the extracted polyphenol with a macroporous adsorbent resin, and carrying out vacuum concentration to a dry state to obtain the purified polyphenol. The method has the advantages of high extraction rate and recoverable organic solvent; water in the extracting solution does not need to be evaporated by heating; the room-temperature or low-temperature treatment is beneficial to protecting the product and saving the energy; the purified product has higher solubility; and the method is convenient for compounding the product, provides a brand-new application for longan seeds, develops four cosmetics with wide market prospects (polyphenol whitening nutritive facial cleanser, polyphenol whitening nutritive toner, polyphenol whitening nutritive lotion and polyphenol whitening nutritive cream), and also provides a wide-prospect way for integrated application and development of longan seeds.
Description
Technical field:
The invention belongs to biomedicine field, be specifically related to a kind of Arillus Longan pit polyphenol method for extraction and purification and preparing the application in polyphenol skin-lightening cosmetic.
Background technology:
Arillus Longan (Dimocarpus longan Lour.) is Sapindaceae (Sapindaceae) Euphoria (Dimocarpus Lour.) evergreen fruit trees.As industrial crops in many countries as there are plantation in China, Thailand, India and Vietnam etc.China is distributed in the provinces and regions such as Fujian, Taiwan, Guangxi, Guangdong, Hainan, Sichuan, Guizhou, Yunnan, and in Fujian, there is large-area plantation in Guangdong, Guangxi.Arillus Longan popular name Arillus Longan, has another name called imperial order, than order, Fructus Litchi slave, Fructus Alpiniae Oxyphyllae, sub-Fructus Litchi, circle eye, river pellet, black horse pearl, swallow ovum, sweet spleen, shark tear, wooden round, embroidery wood group etc., be the famous fruit of south China, its sarcocarp is milky white or pale, and meat band is crisp, fresh and sweet.Arillus Longan, as nourishing health-care food, has the history of more than 1,000 year.Chinese medicine is thought, Arillus Longan can be used for treatment asthenia thin and weak, insomnia, forgetful, palpitation with fear, palpitation with a distress feeling, heart-spleen boosting, fills blood, and calms the nerves.
China is country of origin and the manufacturing country of Arillus Longan, and cultivated area and output account for more than 70% and 50% of the world respectively, is maximum YEAST IN LONGAN PRODUCTION state.Over nearly 10 years, total output increases substantially, and be increased to 110.65 ten thousand tons in 2006 by 36.53 ten thousand tons in 1997, amplification reaches 202.90%, wherein 47.56 ten thousand tons, Guangdong in 2006,38.57 ten thousand tons, Guangxi, 21.28 ten thousand tons, Fujian.Because listing is more concentrated, storing condition is backward and storage and freshness-retaining technology is not enough, only have part Arillus Longan directly to consume as fruit, a large amount of Arillus Longans is for processing Arillus Longan, Arillus Longan sweet can and Arillus Longan cream etc., and remaining pit is all directly abandon as refuse.Arillus Longan pit has hemostasis, analgesic therapy, regulates the flow of vital energy, effect of removing dampness, controls wound hemorrhage, hernia, scabies, eczema and scrofula.Nearest report stone extracts has antioxidation, blood sugar lowering, resisting fatigue and suppression rectum cancer cell hypertrophy isoreactivity.Fixed longan fruit nuclear composition except nutritional labeling be exactly mainly polyphenol, Soong and Barlow utilizes HPLC-ESI-MS multiple techniques to determine in Arillus Longan pit containing 13 kinds of polyphenol such as corilagins, Wang Zhiyuan utilizes same method to determine 17 kinds of polyphenol, and Wu Nini therefrom gets four kinds of compounds such as progallin A.Zheng G. etc. are separated from Arillus Longan pit and obtain gallic acid, corilagin, ellagic acid, progallin A, 1-O-galloyl-β-D-glucopyanosyl, Methy Brevifolin-carboxlate, brevifolin and rhamanopyranosyl ellagic acid; and determining their Scavenging ability, result shows that activity is very strong.
Bibliographical information plant polyphenol has various biological activity, as antioxidation, antibacterial, antiviral, anti-inflammatory, arteriosclerosis, antimutagenic, antitumor etc., and has inhibitory action to multiple enzyme.Containing multiple phenolic compound in the fruit of plant, these compounds can protect it from oxidative damage, suppress the growth of yeast, fungus, virus and antibacterial.In order to comprehensive development and utilization Arillus Longan pit, study the effect of the restraint of tyrosinase of the polyphenol after its polyphenol extraction, purification and purification, and by composite for the polyphenol of purification in cosmetic product.
Extraction conditions for Arillus Longan pit polyphenol has bibliographical information.Optimal conditions as bibliographical information (Wang Zhiyuan etc.) is: acetone concentration 50% (v/v), solid-liquid ratio l:140 (g/mL), extraction temperature 50 DEG C, extraction time 2h, ultrasonic 20min, lixiviate three times, seed of Arillus Longan polyphenol yield is 62.1mg/g (PB method).The optimal conditions of bibliographical information (Wu Lanlan etc.) is: extraction time 106min, and volume fraction of ethanol is 58%, Extracting temperature 64 DEG C, and solid-liquid ratio is 1: 9, and with this understanding, polyphenol yield is 42.690mg/g (Forint phenol method).The method of document (Huang Ruqiang) is at 50 ~ 90 DEG C of reflux, extract, 3 ~ 6h with 3 ~ 10 times 80 ~ 95% (volume fraction) ethanol, extraction into ethyl acetate extracting solution, silicagel column on extract, with petroleum ether-chloroform gradient elution, the polyphenol powder of vacuum concentration, microwave drying.The method of document (Li Qingbiao etc.) is phosphate buffer 60 ~ 80 DEG C of lixiviate 10 ~ 40min with pH9 ~ 11, filters, concentrated filtrate, hydrochloric acid acidizing and precipitation, and vacuum drying precipitation obtains product.
Summary of the invention:
First object of the present invention is to provide a kind of Arillus Longan pit polyphenol method for extraction and purification.
Arillus Longan pit polyphenol method for extraction and purification of the present invention, is characterized in that, comprise the following steps:
(1), extract: first by Arillus Longan pit powder and anhydrous propanone according to mass volume ratio (g/mL) for 1:10 ~ 15 mixed room temperature stirs 1 ~ 2h, upper liquid is abandoned after leaving standstill, then according to Arillus Longan pit powder and extractant mass volume ratio be 1:10 ~ 15 second time add 50 ~ 70% aqueous acetone solution, stirring at room temperature 4 ~ 6h, leave standstill, incline and upper liquid and filter, be aqueous acetone solution 1:10 ~ 15 third time adding 50 ~ 70% again according to Arillus Longan pit powder and extractant mass volume ratio, stirring at room temperature 4 ~ 6h, leave standstill, incline and extracting liquid filtering, merge second, the filtrate of three times, polyphenol is obtained after reclaiming acetone,
(2), purification: the Arillus Longan pit powder in macroporous adsorbent resin and step (1) is 0.7:1 according to dry weight weight ratio, pretreatment is carried out to macroporous adsorbent resin, then the polyphenol that step (1) obtains being diluted with water to concentration is upper prop after 4 ~ 6g/L, carry out eluting with the ethanol that volume fraction is 40 ~ 80%, vacuum concentration is to the dry polyphenol obtaining purification.
Preferably, Arillus Longan pit powder in step (1), its preparation method is: naturally dried by Arillus Longan pit, pulverizes 20 mesh sieves and namely obtains Arillus Longan pit powder.
Recovery acetone described in step (1), is preferably normal pressure rotary evaporation and reclaims acetone.
Macroporous adsorbent resin in step (2), is preferably macroporous adsorbent resin HPD-100 or macroporous adsorbent resin NKA-II.
Macroporous adsorptive resins ratio of height to diameter in step (2) is preferably 10.
In step (2), pretreatment is carried out to macroporous adsorbent resin, concrete grammar is: the volume fraction added in resin column higher than resin bed 10 centimetres is the soak with ethanol more than 4 hours of 95%, release immersion, continue washing to cleaning mixture thin up is not muddy and eluent uv scan must not detect absworption peak with the ethanol that volume fraction is 95%, then be washed with water to ethanol content and be less than 1% (without obvious ethanol abnormal smells from the patient).
Upper prop in step (2), loading flow velocity preferably 1 ~ 2BV/h, loading volume is preferably 6 ~ 8BV; Described use volume fraction be 40 ~ 80% ethanol carry out eluting, elution flow rate is preferably 0.5 ~ 1.5BV/h, and elution volume is preferably 1.5 ~ 2.5BV.
The present invention measures with Forint phenol method and extract the yield of polyphenol and the polyphenol content after purification with macroreticular resin from Arillus Longan pit powder, the polyphenol yield extracted by method of the present invention reaches more than 62mg/g dry fruit core, and the polyphenol content after purification is more than 60%.
Can restraint of tyrosinase effectively by the polyphenol of method extraction purification of the present invention, experiment confirms that the suppression ratio of Arillus Longan pit polyphenol to tryrosinase of 2.0mg/mL purification is more than 51.5%, and the IC of the tryrosinase of cosmetics whitening additive arbutin
50value is 5.3mmol/L (1.46mg/mL).Therefore, second object of the present invention is to provide Arillus Longan pit polyphenol and is preparing the application in polyphenol skin-lightening cosmetic.
Described polyphenol skin-lightening cosmetic, is preferably polyphenol whitening nourishing facial milk, polyphenol whitening nutritive water, polyphenol whitening nutritional breast and polyphenol whitening nourishing cream.
Preferably, the consumption of Arillus Longan pit polyphenol in polyphenol skin-lightening cosmetic is mass percent 0.01 ~ 10.0%.
By method of the present invention from the polyphenol good water solubility after Arillus Longan pit extraction purification, this provides probability for composite in cosmetics.Arillus Longan pit polyphenol has good restraint of tyrosinase effect, very strong effect of scavenging radical and absorbs ultraviolet effect, as plant polyphenol, also there is crease-resistant, antiinflammatory, antibacterial, moisture-keeping function simultaneously, therefore can prepare the skin-lightening cosmetic of multiple efficacies with Arillus Longan pit polyphenol.
Technique effect of the present invention is: polyphenol extraction ratio is high, the easily-recovered organic solvent used, water in extracting solution is without heating evaporation, room temperature or K cryogenic treatment are conducive to protection product and save the energy, after purification, product dissolubility improves, facilitate compound product, and provide a brand-new purposes for Arillus Longan pit, develop the cosmetics that four kinds have market prospect: polyphenol whitening nourishing facial milk, polyphenol whitening nutritive water, polyphenol whitening nutritional breast, polyphenol whitening nourishing cream, also for the integrated application exploitation of Arillus Longan pit provides a kind of approach with bright prospects.
Detailed description of the invention:
Following examples further illustrate of the present invention, instead of limitation of the present invention.
Embodiment 1:
(1), extract: collect Arillus Longan in longan fruit field and process the pit abandoned, naturally dry, pulverized 20 mesh sieves and obtain Arillus Longan pit powder.Get Arillus Longan pit powder 100g in 2000mL there-necked flask, then add 1500mL anhydrous propanone, the agitating device of mounting strap sealing, all the other two mouthfuls of jam-packs, stirring at room temperature 1h, leaves standstill 1h, incline as far as possible and upper liquid (substantially clarify, separately deal with), abandon upper liquid; Second time adds 1500mL 60% acetone, room temperature airtight stirring 6h, and static 1h afterwards, inclines as far as possible and upper liquid and filter; Third time adds 1500mL 60% acetone, room temperature airtight stirring 4h, static 1h, filters; Merge second and third filtrate, normal pressure rotary evaporation reclaims acetone and obtains polyphenol, and it is 5g/L that gained polyphenol is diluted with water to polyphenol concentration, and measuring the total extraction ratio of polyphenol through Forint phenol method is 64.67mg/g dry fruit core.Polyphenol aqueous solution is used for next step purification.
(2), purification: get 70g macroporous adsorbent resin HPD-100, in resin column, (specification is Φ 40 × 400mm) adds 95% soak with ethanol more than 4 hours higher than resin bed 10 centimetres, release immersion, continue to be washed till till the not muddy and eluent uv scan of cleaning mixture thin up in test tube must not detect absworption peak with 95% ethanol, then be washed with water to ethanol content and be less than 1% (without obvious ethanol abnormal smells from the patient).By HPD-100 macroporous adsorptive resins on polyphenol aqueous solution, loading flow velocity 1BV/h, loading volume 6BV, carry out eluting with the ethanol that volume fraction is 70%, elution flow rate 1BV/h, elution volume 1.5BV, vacuum concentration is to doing to obtain purified polyphenol, and polyphenol content is 62.37%.
The polyphenol restraint of tyrosinase evaluation of effect of extraction purification: with the L-DOPA of 1.0mmo1/L for substrate.First the L-DOPA (being dissolved in the phosphate buffered solution of pH=6.8) of 1.0mL3mol/L is placed in cuvette, add the phosphate buffered solution of 0.85mL pH=6.8, constant temperature l0min in 30 DEG C of waters bath with thermostatic control, add the tryrosinase aqueous solution of 1.0mL purified polyphenol solution (being dissolved in the phosphate buffered solution of pH=6.8) and 0.15mL 0.15g/L, measure OD
475.This surveys in live body system, and the whole mass concentration of enzyme is 7.5mg/L.Can formula be defined as to the relative inhibition (I) of enzyme:
In formula: OD
lrefer to the absorbance of the survey live body system containing substrate, tryrosinase, purified polyphenol; OD
2refer to containing substrate, tryrosinase, but not containing the absorbance of the survey live body system of purified polyphenol; OD
3refer to containing substrate, purified polyphenol, but not containing the absorbance of the survey live body system of tryrosinase; OD
4refer to containing substrate, but not containing the absorbance of the survey live body system of tryrosinase, purified polyphenol.
Result is the suppression ratio of polyphenol to tryrosinase of 2mg/mL extraction purification is 53.97%.
Embodiment 2:
(1), extract: collect Arillus Longan in longan fruit field and process the pit abandoned, naturally dry, pulverized 20 mesh sieves and obtain Arillus Longan pit powder.Get Arillus Longan pit powder 100g in 2000mL there-necked flask, then add 1200mL anhydrous propanone, the agitating device of mounting strap sealing, all the other two mouthfuls of jam-packs, stirring at room temperature 2h, leaves standstill 1h, incline as far as possible and upper liquid (substantially clarify, separately deal with), abandon upper liquid; Second time adds 1500mL 55% acetone, room temperature airtight stirring 5h, and static 1h afterwards, inclines as far as possible and upper liquid and filter; Third time adds 1000mL 55% acetone, room temperature airtight stirring 4h, static 1h, filters; Merge second and third filtrate, normal pressure rotary evaporation reclaims acetone and obtains polyphenol, and it is 4g/L that gained polyphenol is diluted with water to polyphenol concentration, and measuring the total extraction ratio of polyphenol through Forint phenol method is 63.58mg/g dry fruit core.Polyphenol aqueous solution is used for next step purification.
(2), purification: get 70g macroporous adsorbent resin NKA-II, in resin column, (specification is Φ 40 × 400mm) adds 95% soak with ethanol more than 4 hours higher than resin bed 10 centimetres, release immersion, continue to be washed till till the not muddy and eluent uv scan of cleaning mixture thin up in test tube must not detect absworption peak with 95% ethanol, then be washed with water to ethanol content and be less than 1% (without obvious ethanol abnormal smells from the patient).By NKA-II macroporous adsorptive resins on polyphenol aqueous solution, loading flow velocity 1.5BV/h, loading volume 8BV, carry out eluting with the ethanol that volume fraction is 80%, elution flow rate 0.6BV/h, elution volume 2BV, vacuum concentration is to doing to obtain purified polyphenol, and polyphenol content is 63.19%.
The polyphenol restraint of tyrosinase evaluation of effect of extraction purification: method is with the method in embodiment 1.
Result is the suppression ratio of polyphenol to tryrosinase of 2mg/mL extraction purification is 55.23%.
Embodiment 3:
(1), extract: collect Arillus Longan in longan fruit field and process the pit abandoned, naturally dry, pulverized 20 mesh sieves and obtain Arillus Longan pit powder.Get Arillus Longan pit powder 100g in 2000mL there-necked flask, then add 1000mL anhydrous propanone, the agitating device of mounting strap sealing, all the other two mouthfuls of jam-packs, stirring at room temperature 1h, leaves standstill 1h, incline as far as possible and upper liquid (substantially clarify, separately deal with), abandon upper liquid; Second time adds 1000mL 50% acetone, room temperature airtight stirring 4h, and static 1h afterwards, inclines as far as possible and upper liquid and filter; Third time adds 1000mL 50% acetone, room temperature airtight stirring 6h, static 1h, filters; Merge second and third filtrate, normal pressure rotary evaporation reclaims acetone and obtains polyphenol, and it is 6g/L that gained polyphenol is diluted with water to polyphenol concentration, and measuring the total extraction ratio of polyphenol through Forint phenol method is 62.23mg/g dry fruit core.Polyphenol aqueous solution is used for next step purification.
(2), purification: get 70g macroporous adsorbent resin NKA-II, in resin column, (specification is Φ 40 × 400mm) adds 95% soak with ethanol more than 4 hours higher than resin bed 10 centimetres, release immersion, continue to be washed till till the not muddy and eluent uv scan of cleaning mixture thin up in test tube must not detect absworption peak with 95% ethanol, then be washed with water to ethanol content and be less than 1% (without obvious ethanol abnormal smells from the patient).By NKA-II macroporous adsorptive resins on polyphenol aqueous solution, loading flow velocity 2BV/h, loading volume 6BV, carry out eluting with the ethanol that volume fraction is 40%, elution flow rate 0.5BV/h, elution volume 2.5BV, vacuum concentration is to doing to obtain purified polyphenol, and polyphenol content is 60.73%.
The polyphenol restraint of tyrosinase evaluation of effect of extraction purification: method is with the method in embodiment 1.
Result is the suppression ratio of polyphenol to tryrosinase of 2mg/mL extraction purification is 51.83%.
Embodiment 4:
(1), extract: collect Arillus Longan in longan fruit field and process the pit abandoned, naturally dry, pulverized 20 mesh sieves and obtain Arillus Longan pit powder.Get Arillus Longan pit powder 100g in 2000mL there-necked flask, then add 1500mL anhydrous propanone, the agitating device of mounting strap sealing, all the other two mouthfuls of jam-packs, stirring at room temperature 2h, leaves standstill 1h, incline as far as possible and upper liquid (substantially clarify, separately deal with), abandon upper liquid; Second time adds 1500mL 70% acetone, room temperature airtight stirring 4h, and static 1h afterwards, inclines as far as possible and upper liquid and filter; Third time adds 1500mL 70% acetone, room temperature airtight stirring 4h, static 1h, filters; Merge second and third filtrate, normal pressure rotary evaporation reclaims acetone and obtains polyphenol, and it is 4g/L that gained polyphenol is diluted with water to polyphenol concentration, and measuring the total extraction ratio of polyphenol through Forint phenol method is 64.29mg/g dry fruit core.Polyphenol aqueous solution is used for next step purification.
(2), purification: get 70g macroporous adsorbent resin HPD-100, in resin column, (specification is Φ 40 × 400mm) adds 95% soak with ethanol more than 4 hours higher than resin bed 10 centimetres, release immersion, continue to be washed till till the not muddy and eluent uv scan of cleaning mixture thin up in test tube must not detect absworption peak with 95% ethanol, then be washed with water to ethanol content and be less than 1% (without obvious ethanol abnormal smells from the patient).By HPD-100 macroporous adsorptive resins on polyphenol aqueous solution, loading flow velocity 1BV/h, loading volume 6BV, carry out eluting with the ethanol that volume fraction is 50%, elution flow rate 1.5BV/h, elution volume 2.5BV, vacuum concentration is to doing to obtain purified polyphenol, and polyphenol content is 61.24%.
The polyphenol restraint of tyrosinase evaluation of effect of extraction purification: method is with the method in embodiment 1.
Result is the suppression ratio of polyphenol to tryrosinase of 2mg/mL extraction purification is 52.66%.
Embodiment 5:
1, the preparation of Arillus Longan pit polyphenol whitening nourishing facial milk
The composition proportion of table 1. Arillus Longan pit polyphenol whitening nourishing facial milk
| Raw material | Consumption/% |
| Arillus Longan pit polyphenol | 0.01～10.0 |
| Alkyl alcohol ethoxylates sulfate ammonium salt | 1.0～30.0 |
| Sodium lauroyl sarcosine | 1.0～20.0 |
| Hydroxyethyl ether cellulose | 0.1～5.0 |
| PEG7-glyceryl cocoate | 0.1～10.0 |
| Glycerol | 0.1～20.0 |
| Essence | 0.01～4.0 |
| Antiseptic | 0.1～1.0 |
| Water | 77.6 |
Arillus Longan pit polyphenol (in embodiment 1 extraction purification gained) 3.0%, alkyl alcohol ethoxylates sulfate ammonium salt 8%, sodium lauroyl sarcosine 8.0%, hydroxyethyl ether cellulose 1.0%, PEG7-glyceryl cocoate 1.0%, glycerol 1.0%, essence 0.2%, antiseptic 0.2%, water 77.6%.
Concrete preparation method is: hydroxyethyl ether cellulose and water mix, stirring and dissolving, add alkyl alcohol ethoxylates sulfate ammonium salt, sodium lauroyl sarcosine, PEG-7 glyceryl cocoate, glycerol, be heated to 70 DEG C, stirring and dissolving, when stirring that to be cooled to temperature be 45 DEG C, adds Arillus Longan pit polyphenol, essence, antiseptic, stir, discharging obtains Arillus Longan pit polyphenol whitening nourishing facial milk.
2, Arillus Longan pit polyphenol whitening nutritive water
The composition proportion of table 2. Arillus Longan pit polyphenol whitening nutritive water
| Raw material | Consumption/% |
| Arillus Longan pit polyphenol | 0.01～10.0 |
| Asiaticoside | 0.1～2.0 |
| Hyaluronate sodium | 0.01～1.0 |
| Glucosan | 0.05～2.0 |
| Glycerol | 0.1～20.0 |
| Water | 30～100 |
| Disodiumedetate | 0.01～0.5 |
| Triethanolamine | 0.01～1.0 |
| Antiseptic | 0.1～0.6 |
| Cremophor RH40 | 0.01～2.0 |
| Essence | 0.01～1.0 |
Arillus Longan pit polyphenol (in embodiment 2 extraction purification gained) 3.0%, asiaticoside 0.5%, hyaluronate sodium 0.1%, glucosan 0.5%, glycerol 10.0%, disodiumedetate 0.05%, triethanolamine 0.2%, antiseptic 0.2%, Cremophor RH40 2.0%, essence 0.2%, water to 100%.
Cremophor RH40, essence are mixed water-soluble, then added by other various raw material, stirring and dissolving, and discharging obtains Arillus Longan pit polyphenol whitening nutritive water.
3, Arillus Longan pit polyphenol whitening nutritional breast
The composition proportion of table 3. Arillus Longan pit polyphenol whitening nutritional breast
| Raw material | Consumption/% |
| Arillus Longan pit polyphenol | 0.01～10.0 |
| Asiaticoside | 0.1～2.0 |
| Carbomer | 0.2～1.0 |
| Propylene glycol | 0.05～2.0 |
| Disodiumedetate | 0.01～0.5 |
| Water | 30～100 |
| The stearic alcohol ether of PEG-21 | 0.1～4.0 |
| The stearic alcohol ether of PEG-2 | 0.1～4.0 |
| Tristerin | 0.1～4.0 |
| Polydimethylsiloxane | 0.1～10.0 |
| Isopropyl palmitate | 0.1～10.0 |
| Cetearyl alcohol | 0.1～10.0 |
| Dibenzylatiooluene | 0.01～0.5 |
| Triethanolamine | 0.2～1.0 |
| Antiseptic | 0.1～0.6 |
| Essence | 0.01～1.0 |
Arillus Longan pit polyphenol (in embodiment 3 extraction purification gained) 3.0%, carbomer 0.4%, asiaticoside 0.5%, propylene glycol 4.0%, the stearic alcohol ether 2.0% of disodiumedetate 0.1%, PEG-21, the stearic alcohol ether 1.5% of PEG-2, tristerin 1.0%, polydimethylsiloxane 3.0%, isopropyl palmitate 4.0%, cetearyl alcohol 0.5%, dibenzylatiooluene 0.1%, triethanolamine 0.4%, essence 0.1%, antiseptic 0.3%, adds water to 100%.
Card ripple is scattered in glycerol, then adds water, disodiumedetate, the stearic alcohol ether of PEG-21, high-speed stirred, and be heated to 80 DEG C, obtain aqueous phase; Oil phase (PEG-2 stearic alcohol ether, tristerin, polydimethylsiloxane, isopropyl palmitate, cetearyl alcohol, dibenzylatiooluene) is heated with stirring to 80 DEG C, oil phase is melted; Oil phase is added in aqueous phase, constant temperature high-speed stirred; Add triethanolamine homogenizing; Stirring is cooled to about 45 DEG C, adds Arillus Longan pit polyphenol, asiaticoside, essence, antiseptic; Stir, discharging obtains Arillus Longan pit polyphenol whitening nutritional breast.
4, Arillus Longan pit polyphenol whitening nourishing cream
The composition proportion of table 4. Arillus Longan pit polyphenol whitening nourishing cream
| Raw material | Consumption/% |
| Arillus Longan pit polyphenol | 0.01～10.0 |
| Asiaticoside | 0.1～2.0 |
| Carbomer | 0.2～1.0 |
| Glycerol | 0.1～20.0 |
| Water | 30～100 |
| Disodiumedetate | 0.01～0.5 |
| White mineral oil | 0.1～20.0 |
| Iso-octyl palmitate | 0.1～10.0 |
| Cetearyl alcohol | 0.1～10.0 |
| Polysorbate60 | 0.1～10.0 |
| Span60 | 0.1～10.0 |
| Cera Flava | 0.1～10.0 |
| Dibenzylatiooluene | 0.01～0.5 |
| Triethanolamine | 0.2～1.0 |
| Essence | 0.01～1.0 |
| Antiseptic | 0.1～0.6 |
Arillus Longan pit polyphenol (in embodiment 4 extraction purification gained) 3.0%, carbomer 0.5%, glycerol 3.0%, asiaticoside 0.5%, disodiumedetate 0.1%, white mineral oil 12.0%, iso-octyl palmitate 7.0%, cetearyl alcohol 2.0%, polysorbate60 3.0%, Span60 2.0%, Cera Flava 4.0%, dibenzylatiooluene 0.1%, triethanolamine 0.5%, essence 0.1%, antiseptic 0.3%, adds water to 100%.
Card ripple is scattered in glycerol, then adds water, disodiumedetate, high-speed stirred, and be heated to 80 DEG C, obtain aqueous phase; Oil phase (white mineral oil, iso-octyl palmitate, cetearyl alcohol, polysorbate60, Span60, Cera Flava, dibenzylatiooluene) is heated with stirring to 80 DEG C, oil phase is melted; Oil phase is added in aqueous phase, constant temperature high-speed stirred; Add triethanolamine homogenizing; Stirring is cooled to about 45 DEG C, adds Arillus Longan pit polyphenol, asiaticoside, essence, antiseptic; Stir, discharging obtains Arillus Longan pit polyphenol whitening nourishing cream.
Claims (10)
1. an Arillus Longan pit polyphenol method for extraction and purification, is characterized in that, comprise the following steps:
(1), extract: be first that 1:10 ~ 15 mixed room temperature stirs 1 ~ 2h by Arillus Longan pit powder and anhydrous propanone according to mass volume ratio, upper liquid is abandoned after leaving standstill, then according to Arillus Longan pit powder and extractant mass volume ratio be 1:10 ~ 15 second time add 50 ~ 70% aqueous acetone solution, stirring at room temperature 4 ~ 6h, leave standstill, incline and upper liquid and filter, be aqueous acetone solution 1:10 ~ 15 third time adding 50 ~ 70% again according to Arillus Longan pit powder and extractant mass volume ratio, stirring at room temperature 4 ~ 6h, leave standstill, incline and extracting liquid filtering, merge second, the filtrate of three times, polyphenol is obtained after reclaiming acetone,
(2), purification: the Arillus Longan pit powder in macroporous adsorbent resin and step (1) is 0.7:1 according to dry weight weight ratio, pretreatment is carried out to macroporous adsorbent resin, then the polyphenol that step (1) obtains being diluted with water to concentration is upper prop after 4 ~ 6g/L, carry out eluting with the ethanol that volume fraction is 40 ~ 80%, vacuum concentration is to the dry polyphenol obtaining purification.
2. Arillus Longan pit polyphenol method for extraction and purification according to claim 1, is characterized in that, Arillus Longan pit powder in step (1), its preparation method is: naturally dried by Arillus Longan pit, pulverizes 20 mesh sieves and namely obtains Arillus Longan pit powder.
3. Arillus Longan pit polyphenol method for extraction and purification according to claim 1, is characterized in that, the recovery acetone described in step (1), for normal pressure rotary evaporation reclaims acetone.
4. Arillus Longan pit polyphenol method for extraction and purification according to claim 1, is characterized in that, the macroporous adsorbent resin in step (2) is macroporous adsorbent resin HPD-100 or macroporous adsorbent resin NKA-II.
5. Arillus Longan pit polyphenol method for extraction and purification according to claim 1, it is characterized in that, the macroporous adsorptive resins ratio of height to diameter in step (2) is 10.
6. Arillus Longan pit polyphenol method for extraction and purification according to claim 1, it is characterized in that, the upper prop in step (2), loading flow velocity is 1 ~ 2BV/h, and loading volume is 6 ~ 8BV.
7. Arillus Longan pit polyphenol method for extraction and purification according to claim 1, is characterized in that, described use volume fraction be 40 ~ 80% ethanol carry out eluting, elution flow rate is 0.5 ~ 1.5BV/h, and elution volume is 1.5 ~ 2.5BV.
8. preparing the application in polyphenol skin-lightening cosmetic by the Arillus Longan pit polyphenol of the method extraction purification of claim 1.
9. Arillus Longan pit polyphenol according to claim 8 is preparing the application in polyphenol skin-lightening cosmetic, it is characterized in that, described cosmetics are polyphenol whitening nourishing facial milk, polyphenol whitening nutritive water, polyphenol whitening nutritional breast and polyphenol whitening nourishing cream.
10. Arillus Longan pit polyphenol according to claim 8 is preparing the application in polyphenol skin-lightening cosmetic, it is characterized in that, the consumption of Arillus Longan pit polyphenol in polyphenol skin-lightening cosmetic is mass percent 0.01 ~ 10.0%.
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| CN116731400B (en) * | 2023-04-04 | 2024-01-30 | 华南农业大学 | Longan seed polyphenol starch active membrane and preparation method and application thereof |
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Effective date of registration: 20200927 Address after: Room 01-08, 05 / F, Baiyun electric technology building, Guangzhou private science and Technology Park, No. 1633 Beitai Road, Baiyun District, Guangzhou City, Guangdong Province 510080 Patentee after: Guangdong Chuangmei Anti-aging Research Co.,Ltd. Address before: 510520 No. 321 Longdong North Road, Guangzhou, Guangdong, Tianhe District Patentee before: GUANGDONG FOOD AND DRUG VOCATIONAL College |