CN107049820A - The application of evodin and rattletop promotor composition in smoothing wrinkle anti-wrinkle product is prepared - Google Patents
The application of evodin and rattletop promotor composition in smoothing wrinkle anti-wrinkle product is prepared Download PDFInfo
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- CN107049820A CN107049820A CN201611168915.9A CN201611168915A CN107049820A CN 107049820 A CN107049820 A CN 107049820A CN 201611168915 A CN201611168915 A CN 201611168915A CN 107049820 A CN107049820 A CN 107049820A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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Abstract
The invention belongs to Chinese medicine and cosmetic field, disclosing a kind of can promote the composition of application on human skin collagen synthesis, the composition includes plant monomer evodin and macrotin, and the ratio of the mass percent of the evodin and macrotin is 0.2~15:1；Evodin and macrotin can stimulate skin fibroblast collagen albumen to generate, and both are used in combination with cooperative effect, can remarkably promote skin collagen synthesis, can effectively smoothing wrinkle is crease-resistant, anti-aging.The present composition can be used for preparing crease-resistant smoothing wrinkle, increase skin elasticity, the cosmetics of delay skin aging, it can be used as the form of cosmetic additive agent, the cosmetics can be all kinds of formulations such as creme, emulsion, facial mask, toner, surfactant, ointment, finish, gel, aerosol, solution or plastics.
Description
Technical field
The invention belongs to Chinese medicine and cosmetic field, it is related to the new application of compound evodin and macrotin, specifically
Disclose evodin and macrotin and combinations thereof and promote skin collagen synthesis and anti crease and anti senile old for smoothing wrinkle
Application in cosmetics.
Background technology
Aging is the inexorable law during biological life, and skin senescence is then table the most obvious in human aging process
One of existing, such as dry skin, elasticity disappear, relaxation is coarse, wrinkle is produced and color spot formation phenomenon.Skin health and its
The collagen meshwork of a large amount of dense regulars is closely related in skin corium, and the content of collagen is up to 80%, wherein I in corium
Based on collagen type.Collagen can be lost in advancing age and gradually, and the moisture of skin is reduced, and elasticity is deteriorated, very
The change of skin structure is the main cause of cutis laxa, wrinkle generation and aging.One of the reason for collagen is lost in is skin
The collagen of skin cell itself synthesis is reduced, therefore improves the content of collagen in skin, to skin wrinkle resisting, anti-aging,
Recover skin elasticity significant.
Collagen is directly added in many anti-wrinkle products in the market, but because the molecular weight of collagen compares
Greatly, Skin Cell absorbs and can be very limited, and also has addition collagen small peptide, effect is very different, while collagen egg
Also there is the rotten risk of denaturation during the use of cosmetics for external use in vain.Therefore how effectively crease-resistant, delay skin aging,
Still there is very big realistic price.
Evodin (Evodine), is also limonin, is primarily present in the seed of rutaceae or fruit, point
Son amount is 470.51, and molecular formula is C26H30O8, structural formula is such as shown in (I).Have now found that it has stronger physiologically active, it is such as anti-
Virus, anti-inflammatory, anti-oxidant, antitumor etc., but be not yet reported that on it in terms of skin wrinkle resisting, anti-aging.
Macrotin (Cimifugin), belongs to chromogen ketone, can be extracted from umbelliferae Saposhnikovia divaricata, its molecule
Amount 306.31, molecular formula is C16H18O6, structural formula is such as shown in (II).It is now discovered that it has antitumor activity, while having antioxygen
Change function, the effect in terms of the anti-ageing cosmetics of waiting for a long time of skin wrinkle resisting is not reported also.
The content of the invention
In order to overcome the drawbacks described above of prior art, the present invention proposes evodin and macrotin and combinations thereof and existed
Dermal layer of the skin fibroblast is activated, and promotes the application in its collagen synthesis, the evodin and macrotin
And combinations thereof can increase the content of collagen in Skin Cell, improve skin elasticity and water-retaining property, desalinate wrinkle, and slow down
Wrinkle is produced and skin senescence process.Evodin and the macrotin active component derives from natural Chinese medicinal herb.
In order to realize foregoing invention purpose, this invention takes following technical scheme：
The present invention proposes a kind of composition, and the composition includes the natural plant monomer with the crease-resistant effect of smoothing wrinkle
Evodin and macrotin；The mass ratio that the evodin and macrotin account for composition is 0.2~15:1；Preferably
For 5:27、5:12、5:7、10:9、5:3、5:2、35:9、20:3、15:1.
Wherein, the natural plant monomer evodin and macrotin are powdered；The natural plant list
Body evodin and macrotin can be dissolved in water or cosmetic base.
The composition can be added in cosmetic base as active skull cap components, and the cosmetics are external preparation for skin
Cosmetics.
The present invention is led to using double luciferase reporter gene plasmids of the people's I-type collagen promoter regulation sequence built
Cross be transfected into after host cell detect luciferase active method, screen and demonstrate cell transcription level can substantially on
The active material of I-type collagen content is adjusted, finds that evodin and macrotin and combinations thereof are promoting application on human skin first
Effect in terms of Collagen of Fibroblasts albumen synthesis.The first public evodin of the present invention and rattletop promotor composition are in skin
Smoothing wrinkle is crease-resistant, the effect in terms of anti-aging.
The invention also provides the composition is in activation dermal layer of the skin fibroblast, its collagen is promoted to close
Into, and prepare that smoothing wrinkle is crease-resistant, the application in anti-aging products；Evodin and macrotin can be cooperateed with the composition
The synthesis of collagen in skin fibroblasts is stimulated, crease-resistant, anti-aging active ingredient can be made an addition to outside as smoothing wrinkle
In applying some make up.
The composition can use any formulation suitable for skin, can such as be made with wrinkle resistant smoothing wrinkle or enhancing skin
Creme, emulsion, facial mask, toner, surfactant, ointment, finish, gel, aerosol, solution or plastics of skin elasticity etc.
All kinds of use formulations.Above-mentioned various types of cosmetics can be prepared according to the conventional method of cosmetic field.Cosmetics are added
The form of agent is used.
The invention also provides evodin is in activation dermal layer of the skin fibroblast, its collagen is promoted to close
Into, and prepare that smoothing wrinkle is crease-resistant, the application in anti-aging products.Wherein, the concentration of the evodin is 50 μ g/mL
When, effect is best.
The invention also provides macrotin is in activation dermal layer of the skin fibroblast, promote its collagen synthesis, with
And prepare that smoothing wrinkle is crease-resistant, the application in anti-aging products.Wherein, when the concentration of the macrotin is 30 μ g/mL, effect is most
It is good.
Wherein, the collagen is synthesized by skin corium fibroblast.
The invention also provides a kind of smoothing wrinkle is crease-resistant, delay skin aging cosmetics, composition as described above accounts for described
The mass percent scope of cosmetics is 0.002%~1.5%；Surplus is cosmetic base.
Preferably, smoothing wrinkle of the invention is crease-resistant, in delay skin aging cosmetics, and composition as described above accounts for described
The mass percent scope of cosmetics is 0.2%；Surplus is cosmetic base.
The invention also provides a kind of smoothing wrinkle is crease-resistant, delay skin aging cosmetics, evodin and macrotin account for institute
The mass percent for stating cosmetics is respectively：Evodin 0.001%~0.75%, macrotin 0.001%~0.75%；It is remaining
Measure as cosmetic base.Preferably, the cosmetics, evodin and macrotin account for the mass percent point of the cosmetics
It is not：Evodin 0.125%, macrotin 0.075%；Surplus is cosmetic base.
Wherein, the cosmetic base is 6% glycerine, and 5% Dormant oils, 4% isopropyl palmitate, 3.5% caprylic/capric is sweet
Oily three esters, 2% stereth -2,2% stereth -21,1.5% cetostearyl alcohol, 1% polyacrylate -13/ is poly- different
Butylene/polysorbate -20,0.8% stearine, 0.2% allantoin, 0.2% methyl hydroxybenzoate, 0.15% pair of (hydroxyl first
Base) imidazolidinyl urea, 0.1% Nipasol, 0.05%EDTA disodiums, remaining is water.
The beneficial effects of the present invention are described evodin and macrotin and combinations thereof can remarkably promote fell
Skin Collagen of Fibroblasts albumen is synthesized, and evodin and macrotin can cooperate with stimulation skin fibroblasts in the composition
The synthesis of middle collagen, can be used for preparing that smoothing wrinkle is crease-resistant, anti-aging products, or crease-resistant, anti-aging as smoothing wrinkle
Active ingredient is made an addition in cosmetics for external use.
Brief description of the drawings
Fig. 1 is influence of the evodin to activity of fibroblasts in vitro.
Fig. 2 is influence of the macrotin to activity of fibroblasts in vitro.
Fig. 3 is each group medicine of embodiment 2 to skin fibroblasts Luciferase experimental results.
Fig. 4 is each group medicine of embodiment 2 to skin fibroblasts Luciferase experimental results.
Fig. 5 is each group medicine of embodiment 2 to skin fibroblasts Luciferase experimental results.
Fig. 6 is influence of each group medicine to human skin fibroblasts I-type collagen transcriptional level.
Fig. 7 is influence of each group medicine to human skin fibroblasts type III collagen transcriptional level.
Fig. 8 is the influence that each group medicine is synthesized to human skin fibroblasts I-type collagen.
Fig. 9 is influence of each group medicine to human skin fibroblasts type III collagen synthesis.
Figure 10 is the influence of evodin, macrotin different ratio composition to cell I-type collagen content.
Figure 11 is the influence of evodin, macrotin different ratio composition to cell type III collagen content.
Figure 12 is influence of each group medicine to nude mice skin elasticity.
Figure 13 is influence of each group medicine to nude mice skin water content.
Figure 14 is influence of each group medicine to I-type collagen content in nude mice skin tissue.
Figure 15 is influence of each group medicine to type III collagen content in nude mice skin tissue.
Embodiment
With reference to specific examples below and accompanying drawing, the present invention is described in further detail.The process of the implementation present invention,
Condition, experimental method etc., are the universal knowledege and common knowledge of this area, this hair in addition to the following content specially referred to
It is bright that content is not particularly limited.
The influence of the evodin of embodiment 1. and macrotin to activity of fibroblasts in vitro.
Well-grown human skin fibroblasts are inoculated in 96 porocyte culture plates, per hole 2.5*104Individual cell, body
Product is 100 μ L, abandons original culture medium after overnight incubation, is separately added into evodin, the μ L of macrotin 100 of pure DMEM dilutions,
Evodin, the concentration of macrotin are respectively 0 μ g/mL, 20 μ g/mL, 50 μ g/mL, 100 μ g/mL, 150 μ g/mL, 200 μ g/
mL、300μg/mL.MTS (U.S. Madison Promega) is added after 24h, is placed in cell culture incubator after 30min, ELIASA inspection
Survey its light absorption value under 490nm.As a result as depicted in figs. 1 and 2, when the concentration of evodin and macrotin is respectively equal to or
During less than 150 μ g/mL, will not influence be produced on cytoactive.
Embodiment 2.Luciferase experimental models screen medicine.
For the crease-resistant effect of the smoothing wrinkle for evaluating evodin of the present invention, macrotin and combinations thereof, and its in evodia rutaecarpa
The synergy of ester and macrotin, following Dual-Luciferase has been carried out using evodin, macrotin and both compositions
Reporter gene assay.Wherein, evodin and macrotin monomer are purchased from Shanghai Shi Dande Co., Ltds.
Human skin fibroblasts are inoculated in 24 orifice plates, per hole 1.5*105Individual cell, volume is 500 μ L, logarithmic growth
Culture medium is changed into 500 μ L in phase after adherent rear transfection I-type collagen promoter plasmid and internal reference plasmid, 24h respectively to contain
In various concentrations (0,10 μ g/mL, 20 μ g/mL, 30 μ g/mL, 40 μ g/mL, 50 μ g/mL, 100 μ g/mL, 150 μ g/mL) evodia rutaecarpa
The activity of luciferase is detected after the DMEM culture mediums of ester, macrotin, every group of 3 multiple holes, administration 12h with Chemiluminescence Apparatus, it is real
Test result as shown in Figures 3 and 4, the optium concentration for showing evodin is 50 μ g/mL, macrotin is 30 μ g/mL.
Similarly, human skin fibroblasts are inoculated in 24 orifice plates, per hole 1.5*105Individual cell, volume is 500 μ L,
After adherent culture medium is changed into 500 μ L with evodia rutaecarpa after transfection I-type collagen promoter plasmid and internal reference plasmid, 24h
The pure DMEM culture mediums of ester, macrotin and both compositions, every group of 3 multiple holes, concentration is optimal dense for gained in above-mentioned experiment
The evodin and the concentration of macrotin spent in the μ g/mL of evodin 50, the μ g/mL of macrotin 30, composition are each respectively
From 50%, i.e. the μ g/mL of evodin 25, the μ g/mL of macrotin 15.The work of luciferase is detected after 12h with Chemiluminescence Apparatus
Property, test result indicates that evodin, macrotin and both compositions can substantially raise I-type collagen promoter and exist
The content of transcriptional level, as a result as shown in figure 5, compared with control group, evodin and macrotin can promote collagen
Synthesis, both combine after can cooperate with promotion collagen synthesis, significant difference, with statistical significance, * *:p<0.01.
The medicine of embodiment 3. is to human skin fibroblasts NTx albumen and the shadow of III collagen type transcriptional level
Ring.
The human skin fibroblasts cultivated in vitro are with every hole 3.5*105The amount of individual cell is inoculated in 12 orifice plates, often
Hole 1mL culture mediums.Changed into after cell attachment respectively containing the μ g/mL of evodin 50, the μ g/mL of macrotin 30 and both combinations
The DMEM culture medium 1mL of thing (including 25 μ g/mL evodins and 15 μ g/mL macrotins), probe into evodin, liter
Numb element and both influences of composition to human skin fibroblasts collagen transcriptional level, experimental result such as Fig. 6, Fig. 7
It has been shown that, show each group medicine to human skin fibroblasts NTx albumen and III collagen type mRNA level in-site all substantially on
Rise, with statistical significance, * with control group comparing difference significantly:p<0.05, * *:p<0.01.
Influence of the medicine of embodiment 4. to human skin fibroblasts I-type collagen and type III collagen content.
Tested, detected after drug-treated, the content for the collagen being secreted into nutrient solution by cell ELISA,
So as to examine medicine that human skin fibroblasts are synthesized with the influence of collagen, experiment is divided into three groups, 12 orifice plates with every hole
3.5*105Individual cell amount inoculation, it is adherent after change into respectively 1mL contain the μ g/mL of evodin 50, the μ g/mL of macrotin 30 with
And the DMEM culture mediums of both compositions (evodin and 15 μ g/mL macrotins that include 25 μ g/mL).Fig. 8 is Wu Zhu
Cornel lactone, macrotin and both compositions synthesize the influence of I-type collagen to human skin fibroblasts, wherein, it is and right
Compare according to group, *:p<0.05, * *:p<0.01, * * *:p<0.001.From figure 8, it is seen that compared with control group, adding 50 μ
I-type collagen content in the experimental group of g/mL evodins, 30 μ g/mL macrotins, cell culture fluid is significantly improved；Plus
The I-type collagen content pole entered in the experimental group of both compositions, cell culture fluid significantly improves (p<0.001).Fig. 9 is Wu
Fruit of medicinal cornel lactone, macrotin and both compositions synthesize the influence of type III collagen to human skin fibroblasts, wherein,
Compared with control group, add 50 μ g/mL evodins, the experimental group of 30 μ g/mL macrotins, type III collagen contains
Amount significantly improves (*:p<0.05)；The type III collagen content added in the experimental group of both compositions, cell culture fluid is carried
It is high obvious, with more significant statistical significance (* *:p<0.01).
The evodin of embodiment 5., macrotin different ratio composition are to cell I-type collagen and type III collagen egg
Bai Hanliang influence.
Tested by cell ELISA, the basic be the same as Example 3 of experimentation, wherein control group drug concentration are 0, by Wu Zhu
The μ g/mL concentration of cornel lactone 50 and the μ g/mL concentration of macrotin 30 are regarded as very respectively, and pharmaceutical composition quality proportioning is respectively 1:
9 (i.e. 5 μ g/mL evodins and 27 μ g/mL macrotins, labeled as composition 1), 2:8 (i.e. 10 μ g/mL evodins and
24 μ g/mL macrotins, labeled as composition 2), 3:7 (i.e. 15 μ g/mL evodins and 21 μ g/mL macrotins, labeled as group
Compound 3), 4:6 (i.e. 20 μ g/mL evodins and 18 μ g/mL macrotins, labeled as composition 4), 5:5 (i.e. 25 μ g/mL Wus
Fruit of medicinal cornel lactone and 15 μ g/mL macrotins, labeled as composition 5), 6:4 (i.e. 30 μ g/mL evodins and 12 μ g/mL rattletops
Element, labeled as composition 6), 7:3 (i.e. 35 μ g/mL evodins and 9 μ g/mL macrotins, labeled as composition 7), 8:2 (i.e.
40 μ g/mL evodins and 6 μ g/mL macrotins, labeled as composition 8), 9:1 (i.e. 45 μ g/mL evodins and 3 μ g/
ML macrotins, labeled as composition 9).Figure 10 is evodin with macrotin different proportion composition to application on human skin into fiber
Cell synthesizes the influence of I-type collagen, and the I-type collagen expression compared with control group in each group cell culture fluid is improved,
Difference has statistical significance, and the wherein p value of composition 5 is less than 0.05, and the p value of remaining composition is less than 0.01, wherein combining
Thing 5, quality proportioning 5:The effect of 5 (i.e. 25 μ g/mL evodins and 15 μ g/mL macrotins) is the most obvious.Figure 11 evodia rutaecarpas
Lactone and macrotin different proportion composition synthesize the influence of type III collagen to human skin fibroblasts, as a result similar
In the influence to I-type collagen, when evodin and macrotin ratio are 5:5 (i.e. 25 μ g/mL evodins and 15 μ
G/mL macrotins) when type III collagen content it is most.
Influence of each group medicine of embodiment 6. to nude mice skin elasticity and water content.
In order to evaluate the crease-resistant effect of smoothing wrinkle of the present invention, following cream is made in evodin and macrotin and combinations thereof
Frost, while blank control sample is made by same procedure.
The plant monomer composition with the crease-resistant effect of smoothing wrinkle of the present embodiment, is calculated, according to following by mass percentage
Component is prepared.Medicine addition concentration is 50 times of cell concentration.
Mass percent is 0.25% evodin, 0.15% macrotin, is containing mass percent by the present embodiment
Composition I (the quality proportionings 3 of 0.075% evodin and 0.105% macrotin:7, computational methods are with reference to embodiment 5), contain
Mass percent is the composition II (quality proportionings 5 of 0.125% evodin and 0.075% macrotin:5, computational methods ginseng
According to embodiment 5), containing the composition III (quality proportionings 8 that mass percent is 0.2% evodin and 0.03% macrotin:
2, computational methods are with reference to embodiment 5) cosmetics paste is made, basic components component ratio is (mass percent)：0.05%
EDETATE SODIUM, 0.2% allantoin, 6% glycerine, 5% Dormant oils, 0.8% stearine, 4% isopropyl palmitate, 3.5%
Caprylic/capric triglyceride, 2% stereth -2,2% stereth -21,0.2% methyl hydroxybenzoate, 0.1% oxybenzene third
Ester, 1.5% cetostearyl alcohol, 1% -13/ polyisobutene of polyacrylate/polysorbate -20,0.15% pair (methylol) miaow
Oxazolidinyl urea, remaining is water (above mass percent be account for institute's cosmetics paste gross mass percentage).
Raw material is commercially available prod, and evodin and macrotin monomer are purchased from Shanghai Shi Dande Co., Ltds.
The cream coating that present embodiment is prepared is uniformly, feel is fine and smooth, easily absorb, and it is elastic and aqueous to carry out nude mice skin
Measure examination, the experiment of nude mice skin tissue collagen protein immunization groupization.
From 6 week old female BAl BIc/C nude mices 20 (be purchased from Shanghai Si Laike Company of Animals Ltd., SPF grades) feed in
In sterile mouse cage, sterile standard feed and physiological saline is fed, mouse cage is regularly replaced.Adaptability is raised 3 days in advance before experiment, right
All operations of nude mice are carried out on superclean bench.Experiment is divided into five groups, every group of 4 nude mices, and first group is in evodia rutaecarpa
Ester group, second group is macrotin group, and the 3rd group is evodin and rattletop promotor composition I groups, the 4th group of evodin and
Rattletop promotor composition II groups, the 5th group of evodin and rattletop promotor composition III groups, in the right half of painting of nude mice skin of back
Cream blank sample is smeared, left half of skin of back only smears each group cream containing medicine.Sooner or later it is each daily to apply once, continue
6 weeks.The nude mice back left and right sides is measured respectively with skin elasticity moisture tester (Cutometer MPA580, Germany) after 6 weeks
The elasticity and water content of skin, as shown in figure 12, each group medicine has obvious increase to experimental result to nude mice skin elasticity, with
Self-controlled group compares, significant difference.Test result indicates that the elasticity of evodin and rattletop promotor composition to mouse skin
Effect becomes apparent from, significantly more difference, is respectively provided with statistical significance, *:p<0.05.
Each drug-treated experimental group is compared with control group, and the water content of mouse skin is also significantly improved, and sees Figure 13, respectively
Group medicine also has obvious influence to nude mice skin water content, is compared with self-controlled group, difference has conspicuousness statistics poor
Different, composition II p value is less than 0.001, and remaining group p value is less than 0.01.
Influence of each group medicine of embodiment 7. to collagen content in nude mice skin.
After the processing of the animal skin administration of 6 weeks, the homogenate of every group of nude mice back or so skin histology is extracted, is used
I, the content of type III collagen in ELISA method detection each group mice skin tissue.As a result Figure 14, Figure 15 are seen.In evodia rutaecarpa
Ester group, macrotin group, composition I groups, composition II groups and composition III groups (each experimental group is in the same manner as in Example 6) with from
Body control is compared, and all pole significantly increases I, the content of type III collagen, with statistical significance, * * *:p<0.001.
Experiment effect of each medicine group of embodiment 8. to human body skin.
In order to evaluate the crease-resistant effect of smoothing wrinkle of the present invention, evodin, macrotin and combinations thereof cream are subjected to human body
Skin-test.30 each age group volunteers are selected, men and women half and half, is divided into three groups, every group of each 5 men, 5 Mies.
After the daily morning and evening cleaner arm of volunteer, blank cream and composition cream are applied to left and right inner forearm specific bit respectively
Put, and avoid washing, skin elasticity is detected with skin elasticity moisture tester (Cutometer MPA 580, Germany) after 6 weeks
And water content.As a result find, A groups are that to smear evodin group, B groups be that to smear macrotin group, C groups be to smear evodin
With the composition cream group of macrotin, man and Ms do not occur allergic symptom in three groups, are compared with control group, apply
Smearing skin elasticity and water content after evodin, macrotin and both composition creams substantially increases, such as (table 1) institute
Show, significant difference, with statistical significance, wherein, only smear evodin, the p of macrotin group<0.05；Smear evodia rutaecarpa
The cream group p of the composition of lactone and macrotin<0.01.
Influence of the medicine of table 1 to human body skin elasticity and moisture
Note：Compared with control group, *:p<0.05, * *:p<0.01.
The protection content of the present invention is not limited to above example.Under the spirit and scope without departing substantially from inventive concept, this
Art personnel it is conceivable that change and advantage be all included in the present invention, and using appended claims as protect
Protect scope.
Claims (10)
1. a kind of composition, it is characterised in that including evodin and macrotin, the matter of the evodin and macrotin
Amount is than being 0.2~15:1.
2. composition as claimed in claim 1, it is characterised in that the mass ratio of the evodin and macrotin is 5:3.
3. application of the composition as claimed in claim 1 in collagen synthesis is promoted.
4. composition as claimed in claim 1 is preparing that smoothing wrinkle is crease-resistant, the application in delay skin aging cosmetics.
5. evodin is promoting skin collagen synthesis, prepare that smoothing wrinkle is crease-resistant, in delay skin aging cosmetics should
With.
6. macrotin is promoting skin collagen synthesis, prepare that smoothing wrinkle is crease-resistant, the application in delay skin aging cosmetics.
7. the application as described in claim 3,5 or 6, wherein, the collagen is synthesized by skin corium fibroblast.
8. a kind of smoothing wrinkle is crease-resistant, delay skin aging cosmetics, it is characterised in that composition as claimed in claim 1 accounts for institute
The mass percent for stating cosmetics is 0.002%~1.5%.
9. a kind of smoothing wrinkle is crease-resistant, delay skin aging cosmetics, it is characterised in that the cosmetics include evodin and liter
Numb element, the mass percent that the evodin accounts for the cosmetics is 0.001%~0.75%, and the macrotin accounts for described
The mass percent of cosmetics is 0.001%~0.75%.
10. smoothing wrinkle as described in claims 9 or 10 is crease-resistant, delay skin aging cosmetics, it is characterised in that the cosmetic
Product also include cosmetic base, the cosmetic base include 6% glycerine, 5% Dormant oils, 4% isopropyl palmitate, 3.5% is pungent
Acid/Triglyceride DDD, 2% stereth -2,2% stereth -21,1.5% cetostearyl alcohol, 1% polyacrylic acid
The polyisobutene of ester -13//polysorbate -20,0.8% stearine, 0.2% allantoin, 0.2% methyl hydroxybenzoate,
0.15% Bis(hydroxymethyl)imidazolidinyl urea, 0.1% Nipasol, 0.05%EDTA disodiums, remaining is water.
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| JPH06312932A (en) * | 1993-04-28 | 1994-11-08 | Kuraray Co Ltd | External anti-inflammatory preparation |
| CN102000000A (en) * | 2009-09-01 | 2011-04-06 | 刘凤丹 | Application method of tangerine seed oil |
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| 姜超: ""防风中有效成分的提取及对实验性肝损伤保护作用的研究"", 《中国优秀硕士学位论文全文数据库医药卫生科技辑》 * |
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