CN107047962A - It is a kind of to prevent and treat feed addictive that bream red skin disease becomes and its preparation method and application - Google Patents
It is a kind of to prevent and treat feed addictive that bream red skin disease becomes and its preparation method and application Download PDFInfo
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- CN107047962A CN107047962A CN201710343008.1A CN201710343008A CN107047962A CN 107047962 A CN107047962 A CN 107047962A CN 201710343008 A CN201710343008 A CN 201710343008A CN 107047962 A CN107047962 A CN 107047962A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/68—Plantaginaceae (Plantain Family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/254—Acanthopanax or Eleutherococcus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/535—Perilla (beefsteak plant)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The present invention relates to a kind of feed addictive, more particularly to it is a kind of prevent and treat feed addictive that bream red skin disease becomes and its preparation method and application, the feed addictive includes following components in percentage by weight：Asiatic plantain 40 55%, perilla leaf 5 15%, licorice 3 7%, Cortex Acanthopancis extract 15 20%, a starch 10 20%, bentonite 3 6%, allicin 0.3 0.5%.The feed addictive of the present invention can both strengthen bream blood leucocyte content and lifting fish body immunity function, fish body systemic blood circulation can be promoted unobstructed again with improving peripheral blood circulation, becoming position to red skin disease has good repair, there is good prevention and treatment effect to the red leatherware of bream.
Description
Technical field
The present invention relates to a kind of feed addictive, more particularly to it is a kind of prevent and treat feed addictive that bream red skin disease becomes and its
Preparation method and application.
Background technology
Bream (Parabramis pekinensis), also known as bream, also known as long body bream, bream flower, oily bream, with growth it is rapid,
It is adaptable, the features such as feeding habits are wide, meat is soft, delicious flavour, is one of main cultured freshwater fish of China.
Practice finds that the phenomenon that red skin occurs for bream is very universal, is mainly shown as fish body part or most of rubescent, body
Table and fin ray locality are most of congested in various degree, and processing is not in time or when dealing with improperly, and the symptom of the red skin of bream will be after
Continuous to deteriorate, red skin zone position will gradually be evolved into purulence focus or exedens focus, then easily trigger septicemia and cause bream
It is dead.Analyzed from genesis mechanism, bream is first in the continuous action by factors such as environmental stress, the infection of microorganism locality
Under, there is inflammatory congestion in periphery capillary, even inflammatory hemorrhage lesion, finally because scabies secondary infection causes that sb.'s illness took a turn for the worse and causes
Extremely.Therefore, bream red skin disease lesion is prevented and treated by safe and efficient measure has product to reducing the death caused by red skin deteriorates
Pole meaning.
The content of the invention
The present invention is acted synergistically by each side such as Immune-enhancing effect, inflammation elimination and blood circulation improvement, can be effective
The generation of red skin disease change is prevented and treated, also can effectively alleviate the deterioration of red skin disease feelings, promotes bream body health.
In a first aspect, the present invention provides a kind of feed addictive for preventing and treating the change of bream red skin disease, the feed addictive
Include following components in percentage by weight：Asiatic plantain 40-55%, perilla leaf 5-15%, licorice 3-7%, cortex acanthopanacis is carried
Take thing 15-20%, a- starch 10-20%, bentonite 3-6%, allicin 0.3-0.5%.
Present invention feed addictive with prophylactic-therapeutic effect based on bream red skin disease genesis mechanism and symptom reparation screening, it is right
The red skin disease of bream and similar fish has good prevention effect.
It is preferred that, a kind of to prevent and treat the feed addictive that bream red skin disease becomes, the feed addictive includes following weight hundred
Divide the component of ratio：Asiatic plantain 45-52%, perilla leaf 12-15%, licorice 3-5%, Cortex Acanthopancis extract 15-20%, a-
Starch 10-12%, bentonite 4.5-5.5%, allicin 0.4-0.5%.
In a preferred embodiment, it is a kind of to prevent and treat the feed addictive that bream red skin disease becomes, the feed addictive
Consist of the following components in percentage by weight：Asiatic plantain 45%, perilla leaf 15%, licorice 5%, Cortex Acanthopancis extract
20%, a- starch 10%, bentonite 4.5%, allicin 0.5%.
In another preferred embodiment, it is a kind of to prevent and treat the feed addictive that bream red skin disease becomes, the feed addition
Agent is consisted of the following components in percentage by weight：Asiatic plantain 52%, perilla leaf 12%, licorice 3%, Cortex Acanthopancis extract
15%, a- starch 12%, bentonite 5.5%, allicin 0.5%.
The Asiatic plantain of the feed addictive of the present invention and perilla leaf combination, can both strengthen bream blood leucocyte content with carrying
Fish body immunity function is risen, can promote fish body systemic blood circulation unobstructed again and improve peripheral blood circulation, is the preventing and treating red skin of bream
The main component that lesion occurs；Cortex Acanthopancis extract and licorice, becoming position to red skin disease has good repair,
There is Synergistic function to Asiatic plantain and perilla leaf, be the synergistic component for treating red skin disease change；And bentonite can prolong
The action time of long feed addictive.
Wherein, the Asiatic plantain is preferably Chinese herbaceous peony grass meal, further preferably crushes and obtains through pulverizers more than 300 mesh.
In a preferred embodiment, the Chinese herbaceous peony grass meal is to smash Asiatic plantain by pulverizers more than 60 mesh
Afterwards, then using pulverizer separating twices more than 300 mesh obtain.
Wherein, after the licorice is the ethers organic matter backflow of radix glycyrrhizae, then the extraction of alcohol type organic is used to obtain
Extract.
Further, the licorice is prepared by the method comprised the steps of：By radix glycyrrhizae：Ethers is organic
Thing is with 1：8-20 w/v mixing, is heated to reflux, collects residue, residue is dissolved in water, and is extracted with alcohol type organic,
It is evaporated, obtains licorice.
Wherein, the licorice is prepared by the method comprised the steps of：By radix glycyrrhizae：Ethers organic matter with
1：8-20 w/v mixing, is heated to reflux, collects residue, and fully dissolved with water by residue, then by water and
Alcohol type organic by volume 1：1-2 is dissolved, and oil phase is separated with aqueous phase, and oil phase, reject aqueous phase, by oil phase is collected by filtration
Load Rotary Evaporators to be handled, until being evaporated in oil phase, obtained decorating film as licorice.
In a preferred embodiment, the licorice is prepared by the method comprised the steps of：Will
Radix glycyrrhizae：Ethers organic matter is with 1：8-20 w/v mixing, is heated to reflux 40-45 minute, collects residue, and by residue and
Water is with 1：16-40 volume ratios are fully dissolved, then by water and alcohols organic matter volume ratio 1：1-2 is dissolved, and makes oil phase
Separated with aqueous phase, oil phase is collected by filtration, reject aqueous phase is handled oil phase loading Rotary Evaporators, until the liquid in oil phase
Mutually it is evaporated, obtained decorating film as licorice.
Wherein, described ethers organic matter includes but is not limited to methyl ether, ether.
Wherein, described alcohol type organic includes but is not limited to ethanol, butanol.
In a preferred embodiment, the licorice is prepared by the method comprised the steps of：Will
Radix glycyrrhizae：Ether is with 1：8-14 w/v mixing, is heated to reflux 40-45 minutes, collects residue, and by residue and water with 1：
16-40 volume ratios are fully dissolved, then by water and n-butanol volume ratio 1：1-2 is dissolved, and oil phase is separated with aqueous phase,
Oil phase is collected by filtration, oil phase loading Rotary Evaporators are handled, until the liquid phase in oil phase is evaporated, obtained by reject aqueous phase
Decorating film be licorice.
In a preferred embodiment, the licorice is prepared by the method comprised the steps of：Will
Radix glycyrrhizae：Ether is with 1：10 w/v mixing, is heated to reflux 45 minutes, collects residue, and by residue and water with 1：20 bodies
Product is dissolved than fully, then by water and n-butanol volume ratio 1：1 is dissolved, and oil phase is separated with aqueous phase, is collected by filtration
Oil phase, reject aqueous phase is handled oil phase loading Rotary Evaporators, until the liquid phase in oil phase is evaporated, obtained decorating film
As licorice.
Wherein, the number of times of above-mentioned alcohol type organic dissolving can be 1-3 times, such as 2 times.
Wherein, after the Cortex Acanthopancis extract is the ethers organic matter backflow of cortex acanthopanacis, then using the extraction of alcohol type organic
Obtained extract.
Wherein, the Cortex Acanthopancis extract is prepared by the method comprised the steps of：By cortex acanthopanacis：Ethers is organic
Thing is with 1：8-20 w/v mixing, is heated to reflux, residue is dissolved in water, and is extracted with alcohol type organic, is evaporated, obtains
Cortex Acanthopancis extract.
In a preferred embodiment, the Cortex Acanthopancis extract is prepared by the method comprised the steps of：
By cortex acanthopanacis：Ethers organic matter is with 1：8-20 w/v mixing, is heated to reflux, collects residue, and enter with water by residue
Row fully dissolving, it is then dissolved, oil phase is separated with aqueous phase, oil phase, reject water is collected by filtration with alcohol type organic
Phase, will dissolve as point being evaporated, obtained decorating film as Cortex Acanthopancis extract.
In a preferred embodiment, the Cortex Acanthopancis extract is prepared by the method comprised the steps of：
By cortex acanthopanacis：Ethers organic matter is with 1：8-20 w/v mixing, is heated to reflux, and collects residue, and by residue and water with
1：16-40 volume ratios are fully dissolved, and then it is dissolved with alcohol type organic, oil phase is separated with aqueous phase, is collected by filtration
Oil phase, reject aqueous phase is handled oil phase loading Rotary Evaporators, until the liquid phase in oil phase is evaporated, obtained decorating film
As Cortex Acanthopancis extract.
In a preferred embodiment, the Cortex Acanthopancis extract is prepared by the method comprised the steps of：
By cortex acanthopanacis：Ethers organic matter is with 1：8-20 w/v mixing, is heated to reflux 40-45 minutes, collects residue, and by residual
Slag is with water with 1：16-40 volume ratios are fully dissolved, then by water and alcohols organic matter volume ratio 1：1-2 is dissolved, and is made
Oil phase is separated with aqueous phase, and oil phase is collected by filtration, and reject aqueous phase is handled oil phase loading Rotary Evaporators, until in oil phase
Liquid phase be evaporated, obtained decorating film as Cortex Acanthopancis extract.
Wherein, described ethers organic matter includes but is not limited to methyl ether, ether.
Wherein, described alcohol type organic includes but is not limited to ethanol, butanol.
In a preferred embodiment, the Cortex Acanthopancis extract is prepared by the method comprised the steps of：
By cortex acanthopanacis：Ether is with 1：The w/v mixing of (8-14), is heated to reflux 40-45 minute, collects residue, and by residue and
Water is with 1：(16-40) volume ratio is fully dissolved, then by water and n-butanol volume ratio 1：(1-2) is dissolved, and makes oil phase
Separated with aqueous phase, oil phase is collected by filtration, reject aqueous phase is handled oil phase loading Rotary Evaporators, until the liquid in oil phase
Mutually it is evaporated, obtained decorating film as Cortex Acanthopancis extract.
Wherein, the number of times of above-mentioned alcohol type organic dissolving can be 1-3 times, such as 2 times.
Wherein, the perilla leaf is perilla leaf of the water content within 8%.
Wherein, the a- starch is corn a- starch.
Wherein, described allicin is flavouring, and preferably allyl sulfide generation sub- sulphur ester propyl ester content is big more than 55%
Allicin.
Wherein, the technical characteristic of above-mentioned additive of the invention can be with any combination.
In the second aspect of the present invention, the present invention provides a kind of feed for preparing a kind of above-mentioned preventing and treating bream red skin disease change
The method of additive, the raw material components are mixed, and are dried to water content 8-9.5%, are obtained the feed addictive.
Wherein, in the above-mentioned methods, drying is using 35 DEG C of constant temperature dryings.
In the third aspect of the present invention, the present invention provides the feed addictive that a kind of above-mentioned preventing and treating bream red skin disease becomes
Using the additive is used according to the 0.3-0.5% of feed relative.
In the feed addictive using the present invention, the side that additive of the invention can be fed by being added in feed
Formula is completed；It can also be completed by way of individually feeding.
The feed addictive of the present invention can both strengthen bream blood leucocyte content and lifting fish body immunity function, can promote again
Enter fish body systemic blood circulation unobstructed with improving peripheral blood circulation, becoming position to red skin disease has good repair, right
The red leatherware of bream has good prevention and treatment effect.
Embodiment
The present invention is further described below in conjunction with specific embodiment.
Embodiment 1：
It is a kind of to prevent and treat the feed addictive that bream red skin disease becomes, consist of the following components in percentage by weight：Chinese herbaceous peony grass meal
45%, perilla leaf 15%, licorice 5%, Cortex Acanthopancis extract 20%, a- starch 10%, bentonite 4.5%, allicin
0.5%.
Wherein, licorice is prepared by the method for following steps：By radix glycyrrhizae：Ether is with 1：10 bulking value
Than mixing, it is heated to reflux 45 minutes, collects residue, and by residue and water with 1：20 volume ratios are fully dissolved, then by water
With n-butanol volume ratio 1：1 is dissolved, and oil phase is separated with aqueous phase, and oil phase is collected by filtration, and oil phase is loaded and revolved by reject aqueous phase
Turn evaporimeter to be handled, until the liquid phase in oil phase is evaporated, obtained decorating film as licorice.
Wherein, Cortex Acanthopancis extract is prepared by the method for following steps：By cortex acanthopanacis：Ether is with 1：(8-14's)
W/v is mixed, and is heated to reflux 40-45 minutes, collects residue, and by residue and water with 1：(16-40) volume ratio is filled
Divide dissolving, then by water and n-butanol volume ratio 1：(1-2) is dissolved, and oil phase is separated with aqueous phase, and oil phase is collected by filtration, and is abandoned
Except aqueous phase, oil phase loading Rotary Evaporators are handled, until the liquid phase in oil phase is evaporated, obtained decorating film as slender acanthopanax
Bark extract.
The method for preparing the feed addictive is：Each raw material components are mixed, 35 DEG C of constant temperature dryings to mixture moisture contain
Measure as 8.5%, obtain the feed addictive.
Embodiment 2：
It is a kind of to prevent and treat the feed addictive that bream red skin disease becomes, consist of the following components in percentage by weight：Chinese herbaceous peony grass meal
52%, perilla leaf 12%, licorice 3%, Cortex Acanthopancis extract 15%, a- starch 12%, bentonite 5.5%, allicin
0.5%.
Wherein, licorice is prepared by the method for following steps：By radix glycyrrhizae：Ether is with 1：8-14 weighing body
Product is heated to reflux 40-45 minutes than mixing, collects residue, and press residue with water with 1：16-40 volume ratios are fully dissolved,
Then by water and n-butanol volume ratio 1：1-2 is dissolved, and oil phase is separated with aqueous phase, oil phase is collected by filtration, reject aqueous phase will
Oil phase loads Rotary Evaporators and handled, until the liquid phase in oil phase is evaporated, obtained decorating film as licorice.
Wherein, Cortex Acanthopancis extract is prepared by the method for following steps：By cortex acanthopanacis：Ether is with 1：(8-14's)
W/v is mixed, and is heated to reflux 40-45 minutes, collects residue, and by residue and water with 1：(16-40) volume ratio is filled
Divide dissolving, then by water and n-butanol volume ratio 1：(1-2) is dissolved, and oil phase is separated with aqueous phase, and oil phase is collected by filtration, and is abandoned
Except aqueous phase, oil phase loading Rotary Evaporators are handled, until the liquid phase in oil phase is evaporated, obtained decorating film as slender acanthopanax
Bark extract.
The method for preparing the feed addictive is：Each raw material components are mixed, 35 DEG C of constant temperature dryings to mixture moisture contain
Measure as 8.5%, obtain the feed addictive.
Test example 1：To the preventive effect of the red skin of pond culture bream
Choose 9 mouthfuls of bream cultivating pools and be used as pilot, 100 ± 15g/ of bream body weight specification in changzhou cultivation base
Tail, the daily commercial feed fed is identical feed brand and identical nutrition class.
9 oral examination point ponds are randomly divided into tri- groups of A, B, C, every group divide into three it is parallel, in the way of being satiated with food and feeding, day throws
A point breakfast, lunch and dinner progress in the morning, afternoon and evening is fed, wherein three parallel ponds of A groups feed the commercial feed of the feed addition prepared containing embodiment 1, B
Three parallel ponds of group feed the commercial feed of the feed addictive of the preparation of embodiment 2, and three parallel ponds of C groups, which feed to be free of, appoints
The commercial feed of what additive, three groups of commercial feeds fed feed identical feed, 20 weeks test periods.Test group details are such as
Shown in table 1.
Table 1：
During experiment, the 5th week, 10 weeks, 15 weeks and 20 weeks respectively in experiment is drawn in the net in each pilot pond progress part, often
The random tail bream of picking 100 in the pool, dress well-bucket is centrifuged 1 minute, is rested 30 minutes in oxygen supply water body environment, observes the number of the red skin of bream
Amount, to judge with the addition of each group pond feed to reducing the effect of the red skin incidence of bream, as a result as shown in table 2.
Table 2：
From table 2 it can be seen that it is continuous using the commercial feed containing the feed addictive prepared by implementation 1 and embodiment 2,
The red skin incidence of bream is in obvious change of successively decreasing, and control group feeds the bream of the commercial feed without feed addictive of the present invention
Fish, red skin hair rate is still apparently higher than A groups and B groups.
From the above results, feed addictive prepared by the present invention is added in bream commercial feed, for by drawing in the net
Or operation stress caused by the red skin of bream have obvious preventive effect.
Test example 2：The preventive effect for the red skin of bream that stress be caused to cultivation operation
Changzhou raiser selects adjacent two mouthfuls of bream fishing breeding ponds, and two mouthfuls of pond breams and set are breeded fish the putting of class
Foster density is identical, and the feed brand fed is identical with nutrition class.
Pool bream average weight specification is deposited for two/tails of 8-9, at first 20 days of bream list marketing, wherein a bite pond is chosen
The pool continuously feeds the commercial feed of the feed addictive prepared containing embodiment 1, and the additive capacity of additive is 0.35%, and another
A bite pond then continuously feeds the commercial feed without additive, is used as control.
Feed end cycle within 20 days, stop feeding 1 day, hold back net and temporarily support 1 day, the 3rd day fish selects beautiful fish through scene with reference to market
Standard and transportation observation, calculate the red skin incidence of bream of different nodes, as a result as shown in table 3.
Table 3：
From table 3 it can be seen that the bream of the continuous commercial feed for feeding the feed addictive prepared containing embodiment 1, fish
Substantially, bream red body, incidence all than the control group more obvious reduction of red skin better than control group of body finish.
As can be known from the above table, the symptom table of the red skin of bream body surface can be caused by holding back net, the operation such as title, fish car and lower fish car excessively
It is existing, analyzed from statistical result, additive of the invention continuously uses some cycles through feed addition, sick generation red to bream
With good preventive effect.
Therapeutic effect of the test example 3 to the red skin of bream
2 mouthfuls of Hubei thing lake area selection grass carp bream polycultured ponds, wherein grass carp are to raise together with kind, and product are supported based on bream
Kind, bream is per mu to be put in a suitable place to breed between quantity 1500-1800 tails, and pond area is between 5-8 mus.Bream body weight specification 85 ± 8g/ tails,
The daily commercial feed fed is identical feed brand and identical nutrition class.
(1) bream red skin disease manages the structure of model
First 2 days of experiment, 2 mouthfuls of ponds stop feeding feed, test the 1st day, are fished at random from each pond by holding back net
Each 300 tail bream, is then lifted by crane 20 seconds by the way that fish is framed up, is weighed 15 seconds, is continued oxygenation and is transported 15 minutes, persistently oxygenation is sudden and violent excessively
Chlorine running water is cleaned, and is transferred in the lasting oxygenation solution containing 0.1% dodecyl sodium sulfate detergent and is handled 1 minute, then turns
Enter to continue the sudden and violent chlorine running water cleaning of oxygenation, remove the mucus that body surface denaturation comes off, finally divide respectively by every 1 group of 100 tail and be put into 9
Test in net cage, totally three groups, every group three parallel, is denoted as a groups, b groups, c groups.
During experiment net cage is put into, rapid examination record bream body surface abnormal conditions, if it was observed that bream body surface goes out
Now irregular or dispersivity plaque-like, point-like are rubescent, then can regard as red skin disease change.Initial red skin incidence during each net cage inlet
Calculated with reference to below equation, its statistical result such as table 4.
Quantity/100 × 100 of the bream of the red skin of red skin incidence (%)=regard as
Table 4：
As shown in table 4, operate and require according to identical, the initial red skin incidence of each group bream is all relatively.
(2) therapeutic effect of the additive to the red skin of bream
Test the 2nd day, that adds prepared by additive, embodiment 2 prepared by embodiment 1 in bream commercial feed respectively adds
Plus agent and the mode without any allogenic material, prepare 2 kinds of testing materials and a kind of blank control group materials, wherein testing material and
Blank control group materials are matched by way of drawing lots at random with experiment net cage, detailed matching result such as table 5：
Table 5：
| Group | Additive | The dosage of additive |
| A groups | Feed addictive prepared by embodiment 1 | 0.4% |
| B groups | Nothing | 0 |
| C groups | Feed addictive prepared by embodiment 2 | 0.5% |
Fed by the testing material of table 5 with the result that match of experiment net cage, the rate that feeds be satiated with food based on.Each net cage is continuously thrown
After feeding 7 days, hauling is carried out to each net cage and fished with a net, the quantity that bream in different net cages still has red skin is recorded, as follows
Calculate red skin therapeutic effect.
Cure rate (%)=(the whole last incidence of the red initial incidence of skin-red skin)/red initial incidence × 100 of skin
Table 6：
As shown in table 5, the bream become through manual-induced red skin disease is continuously fed be mixed with additive of the present invention feed and
The feed of zero addition additive of the present invention 7 days, finds, the red skin of bream between different groups through carrying when net is fished with a net and carries out red skin statistics
There is larger difference in recovery situation, wherein feeding the feed of the additive added with the embodiment of the present invention 1, the preparation of embodiment 2, bream
Red skin change recovery situation is very good, and feeds the feed without additive of the present invention, and the red skin of bream recovers to be delayed
Slowly.
As can be seen here, additive of the present invention is added in feed, becoming for bream red skin disease has good therapeutic effect,
Cure rate is up to 96% or so.
The synergistic function that the active component of test example 4 is controlled the red skin of bream in advance
4 mouthfuls of Hubei thing lake area selection grass carp bream polycultured ponds, wherein grass carp are to raise together with kind, and product are supported based on bream
Kind, bream is per mu to be put in a suitable place to breed between quantity 1500-1800 tails, and pond area is between 5-8 mus.95 ± 12g/ of bream body weight specification
Tail, the daily commercial feed fed is identical feed brand and identical nutrition class.
(1) bream red skin disease manages the structure of model
First 2 days of experiment, 4 mouthfuls of ponds stop feeding feed, test the 1st day, are fished at random from each pond by holding back net
Each 300 tail bream, is then lifted by crane 20 seconds by the way that fish is framed up, is weighed 15 seconds, is continued oxygenation and is transported 15 minutes, persistently oxygenation is sudden and violent excessively
Chlorine running water is cleaned, and is transferred in the lasting oxygenation solution containing 0.1% dodecyl sodium sulfate detergent and is handled 1 minute, then turns
Enter to continue the sudden and violent chlorine running water cleaning of oxygenation, remove the mucus that body surface denaturation comes off, finally divide respectively by every 1 group of 100 tail and be put into 24
In individual experiment net cage, totally 8 groups, every group 3 are parallel, be denoted as A1, A2, A3, B1, B2, B3, C1, C2, C3, D1, D2, D3,
E1、E2、E3、F1、F2、F3、G1、G2、G3、H1、H2、H3。
During experiment net cage is put into, rapid examination record bream body surface abnormal conditions, if it was observed that bream body surface goes out
Now irregular or dispersivity plaque-like, point-like are rubescent, then can regard as red skin disease change.Initial red skin incidence during each net cage inlet
Calculated with reference to below equation, its statistical result such as table 7.
Quantity/100 × 100 of the bream of the red skin of red skin incidence (%)=regard as
Table 7：
| Group | Initial red skin incidence (%) |
| A groups | 32 |
| B groups | 31.7 |
| C groups | 31.3 |
| D | 29.7 |
| E | 32.3 |
| F | 29.7 |
| G | 30 |
| H | 31 |
As shown in table 7, operate and require according to identical, the initial red skin incidence of each group bream is all relatively.
(2) therapeutic effect of the additive to the red skin of bream
Test the 2nd day, add the additive of the preparation of embodiment 1 in bream commercial feed respectively and without any external source
The mode of material, prepares a kind of testing material and a kind of blank control group materials；It is formulated, is prepared based on the addition formula of embodiment 1
The additive that Asiatic plantain micro mist, perilla leaf, licorice and Cortex Acanthopancis extract are individually lacked, lack part is with equivalent flour
Replace；It is formulated again based on the formula of embodiment 1, prepares Asiatic plantain micro mist and perilla leaf missing, licorice and five simultaneously
Plus two groups of additives that bark extract is lacked simultaneously, lack part is with the replacement of equivalent flour.The additive that above-mentioned preparation is completed
0.4% adding proportion admixes in feed the testing material prepared for different groups by weight percentage respectively.Wherein testing material and sky
White control group materials are matched by way of drawing lots at random with experiment net cage, detailed matching result such as table 8：
Table 8：(following table data omit unit：%)
* note：H is blank control group, i.e., without any outer source additive in bream commercial feed, be used as blank control
Group materials.
Fed by the testing material of table 8 with the result that match of experiment net cage, the rate that feeds be satiated with food based on.Each net cage is continuously thrown
After feeding 7 days, hauling is carried out to each net cage and fished with a net, the quantity that bream in different net cages still has red skin is recorded, as follows
Calculate red skin therapeutic effect.
Cure rate (%)=(the whole last incidence of the red initial incidence of skin-red skin)/red initial incidence × 100 of skin
Table 6：
As shown in table 8, after bream becomes through manual-induced red skin disease, under conditions of feeding blank feed at continuous 7 days, bream
Red skin incidence can be made to recover 57.6-69% automatically by itself repair ability, and with the addition of the feeding of feed addition of the present invention
Expect that the red skin recovery progress of bream is very good, red skin repair rate is up to 97%.Therefore, premix of the present invention is added in feed,
The red skin of bream can effectively be treated.
On the other hand, when Asiatic plantain micro mist, perilla leaf are individually lacked, individually missing above two additive is red to bream
The repair of skin has weakened, but when Asiatic plantain micro mist and perilla leaf are lacked simultaneously, corresponding additive is red to bream
The reparation of skin substantially weakens.Thus it is just bright, when Asiatic plantain micro mist, perilla leaf are individually lacked, bream red skin disease is repaired to additive
Effect of change has decrease effect, when Asiatic plantain micro mist and perilla leaf while when lacking, the work(that bream red skin disease becomes is repaired to addition
Can have and more obviously weaken effect.It can be seen that, Asiatic plantain micro mist, perilla leaf repair the red skin effect of bream to additive of the present invention to be had
Synergistic function.
Meanwhile, when radix glycyrrhizae zymotic fluid, cortex acanthopanacis zymotic fluid are individually lacked, individually missing above two additive is to bream
The repair of red skin has weakened, but when radix glycyrrhizae zymotic fluid and cortex acanthopanacis zymotic fluid are lacked simultaneously, corresponding additive
Reparation to the red skin of bream substantially weakens.Thus it is just bright, when radix glycyrrhizae zymotic fluid, cortex acanthopanacis zymotic fluid are individually lacked, to additive
Repairing effect that bream red skin disease becomes has decrease effect, when radix glycyrrhizae zymotic fluid and cortex acanthopanacis zymotic fluid are lacked simultaneously, to addition
Repairing the function of bream red skin disease change has more obviously decrease effect.It can be seen that, radix glycyrrhizae zymotic fluid, cortex acanthopanacis zymotic fluid are to the present invention
Additive, which repairs the red skin effect of bream, has synergistic function.
It is described above, it is only the preferably specific embodiment of the present invention, but protection scope of the present invention is not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Design is subject to equivalent substitution or change, should all cover within the scope of the present invention.
Claims (10)
1. a kind of prevent and treat the feed addictive that bream red skin disease becomes, it is characterised in that the feed addictive includes following weight
The component of percentage：Asiatic plantain 40-55%, perilla leaf 5-15%, licorice 3-7%, Cortex Acanthopancis extract 15-20%,
A- starch 10-20%, bentonite 3-6%, allicin 0.3-0.5%.
2. a kind of feed addictive for preventing and treating the change of bream red skin disease according to claim 1, it is characterised in that the feed
Additive includes following components in percentage by weight：Asiatic plantain 45-52%, perilla leaf 12-15%, licorice 3-5%, five
Plus bark extract 15-20%, a- starch 10-12%, bentonite 4.5-5.5%, allicin 0.4-0.5%.
3. a kind of feed addictive for preventing and treating the change of bream red skin disease according to claim 2, it is characterised in that the feed
Additive is consisted of the following components in percentage by weight：Asiatic plantain 45%, perilla leaf 15%, licorice 5%, cortex acanthopanacis is carried
Take thing 20%, a- starch 10%, bentonite 4.5%, allicin 0.5%.
4. a kind of feed addictive for preventing and treating the change of bream red skin disease according to claim 2, it is characterised in that the feed
Additive is consisted of the following components in percentage by weight：Asiatic plantain 52%, perilla leaf 12%, licorice 3%, cortex acanthopanacis is carried
Take thing 15%, a- starch 12%, bentonite 5.5%, allicin 0.5%.
5. according to the feed addictive that a kind of any described preventing and treating bream red skin disease becomes in Claims 1-4, its feature exists
In the Asiatic plantain micro mist is to crush to obtain through pulverizers more than 300 mesh.
6. according to the feed addictive that a kind of any described preventing and treating bream red skin disease becomes in claim 1 to 5, its feature exists
In the licorice is the n-butanol extract of radix glycyrrhizae；And/or, the Cortex Acanthopancis extract is the n-butanol of cortex acanthopanacis
Extract.
7. the feed addictive that a kind of preventing and treating bream red skin disease according to claim 1 to 6 becomes, it is characterised in that described
Licorice is prepared by the method comprised the steps of：By radix glycyrrhizae：Ethers organic matter is with 1：8-20 bulking value
Than mixing, it is heated to reflux, collects residue, residue is dissolved in water, and extracted with alcohol type organic, is evaporated, obtains licorice.
8. a kind of feed addictive for preventing and treating the change of bream red skin disease according to claim 1, it is characterised in that the slender acanthopanax
Bark extract is prepared by the method comprised the steps of：By cortex acanthopanacis：Ethers organic matter is with 1：8-20 bulking value
Than mixing, it is heated to reflux, residue is dissolved in water, and is extracted with alcohol type organic, is evaporated, obtains Cortex Acanthopancis extract.
9. a kind of prepare according to the feed addictive that a kind of any described preventing and treating bream red skin disease becomes in claim 1 to 8
Method, it is characterised in that mix the raw material components, dries to water content 8-9.5%, obtains the feed addictive.
10. a kind of answering according to the feed addictive that a kind of any described preventing and treating bream red skin disease becomes in claim 1 to 8
With, it is characterised in that the additive is used according to the 0.3-0.5% of feed relative.
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| CN102920990A (en) * | 2012-11-27 | 2013-02-13 | 遵义师范学院 | Chinese herbal medicinal prescription for preventing and treating aeromonas hydrophila fish disease |
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