CN107029248A - 白藜芦醇固体分散体及增加白藜芦醇在红酒中的溶解度的方法 - Google Patents
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Abstract
本发明提出了白藜芦醇固体分散体及增加白藜芦醇在红酒中的溶解度的方法,白藜芦醇固体分散体是以羟丙基‑β‑环糊精(HP‑β‑CD)为载体通过溶剂法制备的,具体步骤为:按质量比,称取白藜芦醇1份、羟丙基‑β‑环糊精4‑5份,在白藜芦醇中加入无水乙醇进行加热溶解,然后加入羟丙基‑β‑环糊精,搅拌溶解,得到混合溶液;把混合溶液置于恒温箱内挥发掉无水乙醇,得到固体混合物质,然后研磨得到固体粉末,即为白藜芦醇固体分散体。白藜芦醇与载体在形成固体分散体时晶型发生了变化,以分子形式存在,形成新的物相,白藜芦醇固体分散体加入到红酒中,从而把白藜芦醇在红酒中的溶解度提高到200‑240mg/100g,显著提高了白藜芦醇在红酒中的溶解度。
Description
技术领域
本发明属于医药技术领域,具体涉及白藜芦醇固体分散体及增加白藜芦醇在红酒中的溶解度的方法。
背景技术
白藜芦醇是一种非黄酮类的酚类物质,为无色针状结晶,难溶于水,易溶于乙醚、氯仿、甲醇、乙醇和丙醇。白藜芦醇广泛存在于葡萄、虎杖、花生、桑椹、松树等天然植物中,它具有保肝利肝、抗炎、抗过敏、抗病原微生物、抗心血管、抗肿瘤等多种有益于人体的生物学作用,白藜芦醇已广泛用于治疗心血管疾病、动脉硬化和高血脂等疾病。目前人们主要通过食用葡萄和红酒来摄入白藜芦醇,但是其中白藜芦醇含量甚微,通常只有 0.5-10 mg/L,且白藜芦醇水溶性低(在水中溶解度为0.03g/L),生物利用度低。
发明内容
为了克服白藜芦醇在红酒中溶解度低的缺点,本发明提出白藜芦醇固体分散体及增加白藜芦醇在红酒中的溶解度的方法,以羟丙基-β-环糊精(HP-β-CD)为载体通过溶剂法,制备白藜芦醇/HP-β-CD固体分散体,显著提高了白藜芦醇在红酒中的溶解度。
为此,本发明采用如下技术方案。
白藜芦醇固体分散体,其特征在于:由白藜芦醇、羟丙基-β-环糊精组成,按质量比,白藜芦醇1份、羟丙基-β-环糊精4-5份;
并通过以下步骤制成:称取白藜芦醇、羟丙基-β-环糊精,在白藜芦醇中加入无水乙醇进行加热溶解,然后加入羟丙基-β-环糊精,搅拌溶解,得到混合溶液;把混合溶液置于恒温箱内在50-60º的温度下挥发掉无水乙醇,得到固体混合物质;把固体混合物质研磨得到固体粉末,即为白藜芦醇固体分散体。
增加白藜芦醇在红酒中的溶解度的方法,其特征在于:(1)制备白藜芦醇固体分散体;(2)把白藜芦醇固体分散体加入到红酒中,从而把白藜芦醇在红酒中的溶解度提高到200-240mg/100g。
本发明的有益效果:本发明以羟丙基-β-环糊精为载体通过溶剂法,制备白藜芦醇固体分散体,白藜芦醇与载体在形成固体分散体时晶型发生了变化,以分子形式存在 ,形成新的物相,然后再把白藜芦醇固体分散体加入到红酒中溶解;本发明采用白藜芦醇固体分散体技术,显著提高白藜芦醇在红酒中溶解度,能把白藜芦醇在红酒中的溶解度提高到200-240mg/100g,扩展了白藜芦醇的应用价值,克服了现有技术中白藜芦醇在红酒中的溶解度低、难以有效利用的缺陷。
附图说明
下面将结合附图,简要分析实验结果
图1是检测白藜芦醇在红酒中的高效液相色谱图。
图2是检测白藜芦醇固体分散体的高效液相色谱图。
图3是白藜芦醇固体分散体体系X衍射图谱。
图4是白藜芦醇固体分散体体系差示扫描量热图谱。
具体实施方式
实施例1
白藜芦醇固体分散体的制备
称取白藜芦醇 500mg,加无水乙醇10-15mL,水浴45-55℃加热溶解,然后加入2500mgHP-β-CD(羟丙基-β-环糊精),搅拌溶解后, 置于恒温箱内在50-60º的温度下挥发掉无水乙醇,得到固体混合物质;研磨得到固体粉末。
实施例2
2.1 溶解度检验
制备饱和白藜芦醇红酒溶液,与白藜芦醇固体分散体溶液,采用HPLC(高效液相色谱法)检测白藜芦醇溶解度,结合图1、图2所示,实验结果见表1。
表1.HPLC测定固体分散体在红酒中的溶解度
白藜芦醇制备成为固体分散体后,红酒中的溶解度提高了42.85倍,效果显著。
2.2 质量检验,验证固体分散体状态,检测白藜芦醇的晶型状态。
以上所诉,充分验证了方案的有效性,其中制备方法为本发明的优选实施方式,应当指出,对于本技术领域的普通技术人员来说,在不脱离本发明技术原理的前提下,还可以做出若干改进和变型,这些改进和变型也应视为本发明的保护范围。
图3中,a.白藜芦醇;b. HP-β-CD; c.白藜芦醇/HP-β-CD固体分散体;d 白藜芦醇/HP-β-CD混合物白藜芦醇有多个特异性衍射峰存在,白藜芦醇/HP-β-CD混合物为白藜芦醇和HP-β-CD的特征峰叠加。将白藜芦醇制备成固体分散体后, 白藜芦醇的特征吸收峰特征吸收峰消失,峰型与HP-β-CD相同。可见,药物与载体在形成固体分散体时晶型发生了变化,以分子形式存在,制备过程中,形成新的物相。
图4中,a.白藜芦醇;b.HP-β-CD; c.白藜芦醇/HP-β-CD固体分散体;d 白藜芦醇/HP-β-CD混合物.白藜芦醇在268.85℃,出现一个尖锐的特征峰。HP-β-CD在94.62 ℃出现一个较宽的特征峰。白藜芦醇/HP-β-CD 物理混合物在70 ℃附近有较宽的特征峰,在244.46℃有较尖锐的特征峰,峰形为两种物质的叠加,分别为HP-β-CD和白藜芦醇的熔点峰。而白藜芦醇/HP-β-CD固体分散体中药物的特征峰则消失。可见,药物与载体在形成固体分散体时晶型发生了变化,以分子形式存在,制备过程中形成新的物相。
Claims (2)
1.白藜芦醇固体分散体,其特征在于:由白藜芦醇、羟丙基-β-环糊精组成,按质量比,白藜芦醇1份、羟丙基-β-环糊精4-5份;
并通过以下步骤制成:称取白藜芦醇、羟丙基-β-环糊精,在白藜芦醇中加入无水乙醇进行加热溶解,然后加入羟丙基-β-环糊精,搅拌溶解,得到混合溶液;把混合溶液置于恒温箱内在50-60º的温度下挥发掉无水乙醇,得到固体混合物质;把固体混合物质研磨得到固体粉末,即为白藜芦醇固体分散体。
2.增加白藜芦醇在红酒中的溶解度的方法,其特征在于:把权利要求1所述的白藜芦醇固体分散体加入到红酒中,从而把白藜芦醇在红酒中的溶解度提高到200-240mg/100g。
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CN110652496A (zh) * | 2019-11-11 | 2020-01-07 | 中山大学 | 一种隐丹参酮的固体分散体及其制备方法和应用 |
CN114191446A (zh) * | 2022-01-24 | 2022-03-18 | 宁夏医科大学 | 含有白藜芦醇的抗疲劳药酒及其制备方法 |
CN114191446B (zh) * | 2022-01-24 | 2023-12-29 | 宁夏医科大学 | 含有白藜芦醇的抗疲劳药酒及其制备方法 |
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